Cobl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cobl
Entrez Gene ID	305497
Full Name	cordon-bleu WH2 repeat protein
General protein information	Preferred Names cordon-bleu WH2 repeat protein Names protein cordon-bleu cordon-bleu homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599225.1	XP_017454714.1	protein cordon-bleu isoform X1
XM_017599226.1	XP_017454715.1	protein cordon-bleu isoform X2
XM_017599227.1	XP_017454716.1	protein cordon-bleu isoform X3
XM_017599228.1	XP_017454717.1	protein cordon-bleu isoform X4
NM_001107236.1	NP_001100706.1	protein cordon-bleu

Pathways from BioSystems

Homologies

	COBL	XP_001082572.2
Bos taurus (cattle)	COBL	XP_003585983.2
Gallus gallus (chicken)	COBL	XP_004935203.1
Pan troglodytes (chimpanzee)	COBL	XP_003318477.1
Rattus norvegicus (Norway rat)	Cobl	NP_001100706.1
Xenopus tropicalis (tropical clawed frog)	cobl	NP_001135515.1
Homo sapiens (human)	COBL	NP_001274365.1
Canis lupus familiaris (dog)	COBL	XP_005631063.1
Mus musculus (house mouse)	Cobl	NP_766084.3

Gene Ontology
Bibliography

Related articles in PubMed

The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.
Hou W, Izadi M, Nemitz S, Haag N, Kessels MM, Qualmann B
PLoS biology13(9)e1002233(2015)

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Revenu C, Ubelmann F, Hurbain I, El-Marjou F, Dingli F, Loew D, Delacour D, Gilet J, Brot-Laroche E, Rivero F, Louvard D, Robine S
Molecular biology of the cell23(2)324-36(2012 Jan)

The functions of the actin nucleator Cobl in cellular morphogenesis critically depend on syndapin I.
Schwintzer L, Koch N, Ahuja R, Grimm J, Kessels MM, Qualmann B
The EMBO journal30(15)3147-59(2011 Jul)

Cordon-bleu is an actin nucleation factor and controls neuronal morphology.
Ahuja R, Pinyol R, Reichenbach N, Custer L, Klingensmith J, Kessels MM, Qualmann B
Cell131(2)337-50(2007 Oct)

Cordon-bleu is a conserved gene involved in neural tube formation.
Carroll EA, Gerrelli D, Gasca S, Berg E, Beier DR, Copp AJ, Klingensmith J
Developmental biology262(1)16-31(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cobl is an actin nucleator controlling neuronal morphology and development.
Title: Cordon-bleu is an actin nucleation factor and controls neuronal morphology.

The following Cobl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cobl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58312	XM_017599225.1 Latest version!	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa58313	XM_017599226.1 Latest version!	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa58314	XM_017599227.1 Latest version!	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa58315	XM_017599228.1 Latest version!	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa11989	NM_001107236.1 Latest version!	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cobl ( XM_017599225.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454714.1 Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58312
Clone ID Related Accession (Same CDS sequence)	XM_017599225.1
Accession Version	XM_017599225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein cordon-bleu isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GCTGAGCATC TTGGGCTGTC GGGCGCAGAT AGCGACGAGG ATCCCTCGAA GTCAGCGTCT 
GGAGGGGACT TGAACGGCTG TGTAACAACA CCCAACTCAC CATCCTTGCA TTCTCGGTCC 
CTGACACTGG GTCCCTCCCT CTCACTGGGC AACATCTCTG GAATGTCTAT GAAGTCAGAC 
ATGAAGAAGC GCAGAGCCCC TCCTCCTCCA AGTCCCGGGC TGCTGGGTCA AGACAAGGTA 
TCAGAAAAGG CCTCCCTCAG CTCGCAGGCC GATCTTCAGA AGAAGAAGAG GCGGGCGCCA 
GCTCCTCCTC CACCACAGCC GCCACCACCA AGTCCTGTGG TTCCCAACCG CAAGGAAGAT 
AAGGAAGAGA ACAGGAAGAG CACAGTGGGT GTTGGACGTC AGGTGCCACA AAAGCCTCCC 
AGAGGCACAG CTCGGGGCCC ACCCCAGTTA GTGCTCCCCC CGCCCCCACC CTATCCTCCT 
CCTGACACAG ATGTAACAGA GCCTGTCACC TTTCCTGGGG AAGGCGCTGG TTCCGAGACC 
TCAGAGCTGA GACCTAAACT GAGTCTGCCT CTGGGGCCTG GCAGCCACTG CAGCATGGGT 
GGAGTCTCAC AGGTGCCCGC GGAGGCTGAG GAGACGGCAT CTGTGGGCAG CTGTTTTGCA 
TCGGAGGACA CAACCGAAGA CTCAGGGGTC ATGAGCTCTC CCTCAGATGT CATCTCCCTG 
GACTCACAAC AAGATAGCAT GAGATCTAAG GATAAATGGT CCACAGACCA GGAGGACTGC 
AGTGACCAGG ACCTGGCTGG AACACCAGAA CTGGGTCCCC AAAAGAGCCC ATCATGGGGG 
AAACATGGCT CAGGAAGCTC AATTTTAAGG ACTGAGAAAG CCGCCATGCC TGGGAATGAT 
GATGAAGATC TGTCCATCAC GGGACTCTTC CATCAGACTC GAGCAGAGCT TGACAAAGAC 
CTGGGAGGGA TGGAAGAAAA TTATGAAACA GATACCAGCT CTCTAACCAG CTCTGTAAAT 
GGGGTATCCA ACCACAGTCT GCAAGAAGCC ATAATCCCTG ATAGTGGTGT GGACGACATC 
CCTGTCACTT TTATCGGGGA AGTTTCTGAC GAGCCTTTCG ACTCGGGGCT GTTCTTCAGT 
GGAAGTAACA ATGCTGCTGC TTTGAACCAA GGGGGCATTG CCAGCCAAAG ATCCCACCTG 
CCCCCATATC AGACTGAGCA GAGCCAGCCA TTCATAAGGA CAAACAGAAA AGAGCCTGAC 
CCCTCCCTTC CTTCCCAAGA TTACAGAAAG AGAAATCAGC CAATCTTAGC CAACACATCT 
GAAAATGAGA ATCCAGTTGG AATAGACCCC AGAGTTACAT CATTTGTTTC AAAGCCTTCG 
ACAGATGACC CAAAGGCAAA GGATAAAGAC AAGATGTGCG GTTCTGGTCC CAGTGAGAAG 
ACACAGACCG GTCACAGAGT AAACTCCCTG CCAGTGAATC CCAGAGTTGG TGAGGATGAA 
AACTCCAACT CAGCCCTGCC CCCATGGTCT CACCATGGCC AGGCCTCGGG GGGAAGCTAT 
GGACTCAAGT ATGGCCTCAC TACATATAAA ATCGTTCCTC CCAAACCAGA GATGAGGTGT 
TATGACAGGG ATGTGTCCCT CTCCACTGGT GCCATTAAGA TTGATGAACT AGGGAATCTG 
ATGAGTCCTC ACATGAATGG AAGCAGGACC ATATCCAAGC CATCAGCTGT TGCCGAAACA 
GAGGCTCCAC CTATTGGAAA AGTCAAAGAG TTCTGGAGGC GCAACTCGAT GGAAAAGTAC 
CTGAATGGGC CAGCCGAGTG CACGGTCAAA AAGGCCCCCT CCACCACCAT CACTGCCACA 
TCCGAAAAAC CACAGCGAGA TGAGACGAAG GCAGGCTTTA CGCTGACCAC TCCGGAGCAG 
CAGCCTGCCT CACAGGAATA CGGGGCACCC CCGGAGGAAG ACAGAAGCCG GCCACATTCA 
GCCGTCTCCT GTCCTGTGAA AGTGCCAGCA CCTAATCCCA CAGACATCAC ATTCCTCAAG 
CCTCAGAGAA GAACATCCAG CCAGTATGTG GCTTCTGCTA TTGCCAAGAA GATGGGACCT 
CCAAAAGTCC ATGCTGATGT GGTGAGACCA CATAAGAAGA CTGCCGAACA AGGCCACGAG 
GAAGCAAAGC TCGCTAGACC ACCTCCAGCC TGGAAAGACA GTGCAGTCCC CAACCTGTGT 
TCAGAGGCCG GCCAGTGTGA GCATGGCACA AACCAGGGCT CTGTCCGCCT TCCCAGCAAC 
CCTGGTGGGC AGTTGCCAGC GGACCATCCC AAAGTAGAGG TCAACAGCAC ATATGGGAAG 
TCAGCGACTC ACAACTCTCC TGCTGCTGTC CACAGAAACT CCTATTTCCT CCCAGGCAGA 
TCTTCCCAGC GGGATCGTGT TTCTGTAGGA CAGAGCTGTG GTTTCCATGA AAAGCAAACT 
ATAAGTAACC AAAAGATGAA TTCAACATCT AACCCCTCGC AGGCACTGGA CAAGGCACAC 
CCAGCCCCCC TGCTCTTGAC AGAAGCCCGT GACTCTGGGA GGATACTGGT GAATGGCTCT 
GCACAGACCC CAGGAAATTG TGAGCCTCCC CACTCTCAGA AAGAGTCCAC CCTGACTAGC 
TATATTATCT TACAGACAGA GGAAAAGCCC AGTCCGTTAT CTGCAGATGG TCAAAACTCA 
GATGATGCTC TACCGTCGAG CATTTTTGGG CCAAAGAAAA AATTCAAGCC TGTCGTTCAA 
AGGCCACTTC CAAAAGATAT ATCCCTGCAC AGTGCCCTGA TGGAAGCCAT CCACACCTCA 
GGAGGGAGGG ACAAGCTCCG AAAGACTGCA GAACAGGCCT CAGAAGGAAG GCCAAAGAAA 
CCATCCTACG TGGAGGCGGA GAGCGAGCGA TCTGCCCTTC TGGCAGCCAT CCGGGGCCAC 
AGTGGGACTC TCAGCCTGAG GAAGGTGTCA TCCCTGGCCT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCTG CACTCATGGC TCCAGGTGTG GACAAGCCTC AGCAAGAAGA CCGTGGCCTT 
CCACCCCCAC CTGCCCTGCC ACCACCATCC ACTCCAGCAT CCCAGGTTCC CTCAGCCTCC 
ATACCGGTTT CTAGGTTCAG CATCGGCACC CTCAGCAACC CAGTAAATGC TAGGCAAGCC 
TTGATGGATG CTATCCGCTC GGGCACAGGA GCTGCAAGAC TCAGAAAGGT TCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454714.1
CDS	1283..5308
Translation	MKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVHLKEALHNSTLDITVVLPSGLEKQSVVSGSRAVMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNAHTVFLKEKVPEEKGKPGLTKAPEKSVRLVVNYLRTQKAVMRVSPEVPLQNILPVICAKCEVSPDHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVLLTKTQSEPSLSCQEMLRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEHLGLSGADSDEDPSKSASGGDLNGCVTTPNSPSLHSRSLTLGPSLSLGNISGMSMKSDMKKRRAPPPPSPGLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQPPPPSPVVPNRKEDKEENRKSTVGVGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVTEPVTFPGEGAGSETSELRPKLSLPLGPGSHCSMGGVSQVPAEAEETASVGSCFASEDTTEDSGVMSSPSDVISLDSQQDSMRSKDKWSTDQEDCSDQDLAGTPELGPQKSPSWGKHGSGSSILRTEKAAMPGNDDEDLSITGLFHQTRAELDKDLGGMEENYETDTSSLTSSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFFSGSNNAAALNQGGIASQRSHLPPYQTEQSQPFIRTNRKEPDPSLPSQDYRKRNQPILANTSENENPVGIDPRVTSFVSKPSTDDPKAKDKDKMCGSGPSEKTQTGHRVNSLPVNPRVGEDENSNSALPPWSHHGQASGGSYGLKYGLTTYKIVPPKPEMRCYDRDVSLSTGAIKIDELGNLMSPHMNGSRTISKPSAVAETEAPPIGKVKEFWRRNSMEKYLNGPAECTVKKAPSTTITATSEKPQRDETKAGFTLTTPEQQPASQEYGAPPEEDRSRPHSAVSCPVKVPAPNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKKTAEQGHEEAKLARPPPAWKDSAVPNLCSEAGQCEHGTNQGSVRLPSNPGGQLPADHPKVEVNSTYGKSATHNSPAAVHRNSYFLPGRSSQRDRVSVGQSCGFHEKQTISNQKMNSTSNPSQALDKAHPAPLLLTEARDSGRILVNGSAQTPGNCEPPHSQKESTLTSYIILQTEEKPSPLSADGQNSDDALPSSIFGPKKKFKPVVQRPLPKDISLHSALMEAIHTSGGRDKLRKTAEQASEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRDAALMAPGVDKPQQEDRGLPPPPALPPPSTPASQVPSASIPVSRFSIGTLSNPVNARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_017599225.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599225.1

ORF Insert Sequence:

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GCTGAGCATC TTGGGCTGTC GGGCGCAGAT AGCGACGAGG ATCCCTCGAA GTCAGCGTCT 
GGAGGGGACT TGAACGGCTG TGTAACAACA CCCAACTCAC CATCCTTGCA TTCTCGGTCC 
CTGACACTGG GTCCCTCCCT CTCACTGGGC AACATCTCTG GAATGTCTAT GAAGTCAGAC 
ATGAAGAAGC GCAGAGCCCC TCCTCCTCCA AGTCCCGGGC TGCTGGGTCA AGACAAGGTA 
TCAGAAAAGG CCTCCCTCAG CTCGCAGGCC GATCTTCAGA AGAAGAAGAG GCGGGCGCCA 
GCTCCTCCTC CACCACAGCC GCCACCACCA AGTCCTGTGG TTCCCAACCG CAAGGAAGAT 
AAGGAAGAGA ACAGGAAGAG CACAGTGGGT GTTGGACGTC AGGTGCCACA AAAGCCTCCC 
AGAGGCACAG CTCGGGGCCC ACCCCAGTTA GTGCTCCCCC CGCCCCCACC CTATCCTCCT 
CCTGACACAG ATGTAACAGA GCCTGTCACC TTTCCTGGGG AAGGCGCTGG TTCCGAGACC 
TCAGAGCTGA GACCTAAACT GAGTCTGCCT CTGGGGCCTG GCAGCCACTG CAGCATGGGT 
GGAGTCTCAC AGGTGCCCGC GGAGGCTGAG GAGACGGCAT CTGTGGGCAG CTGTTTTGCA 
TCGGAGGACA CAACCGAAGA CTCAGGGGTC ATGAGCTCTC CCTCAGATGT CATCTCCCTG 
GACTCACAAC AAGATAGCAT GAGATCTAAG GATAAATGGT CCACAGACCA GGAGGACTGC 
AGTGACCAGG ACCTGGCTGG AACACCAGAA CTGGGTCCCC AAAAGAGCCC ATCATGGGGG 
AAACATGGCT CAGGAAGCTC AATTTTAAGG ACTGAGAAAG CCGCCATGCC TGGGAATGAT 
GATGAAGATC TGTCCATCAC GGGACTCTTC CATCAGACTC GAGCAGAGCT TGACAAAGAC 
CTGGGAGGGA TGGAAGAAAA TTATGAAACA GATACCAGCT CTCTAACCAG CTCTGTAAAT 
GGGGTATCCA ACCACAGTCT GCAAGAAGCC ATAATCCCTG ATAGTGGTGT GGACGACATC 
CCTGTCACTT TTATCGGGGA AGTTTCTGAC GAGCCTTTCG ACTCGGGGCT GTTCTTCAGT 
GGAAGTAACA ATGCTGCTGC TTTGAACCAA GGGGGCATTG CCAGCCAAAG ATCCCACCTG 
CCCCCATATC AGACTGAGCA GAGCCAGCCA TTCATAAGGA CAAACAGAAA AGAGCCTGAC 
CCCTCCCTTC CTTCCCAAGA TTACAGAAAG AGAAATCAGC CAATCTTAGC CAACACATCT 
GAAAATGAGA ATCCAGTTGG AATAGACCCC AGAGTTACAT CATTTGTTTC AAAGCCTTCG 
ACAGATGACC CAAAGGCAAA GGATAAAGAC AAGATGTGCG GTTCTGGTCC CAGTGAGAAG 
ACACAGACCG GTCACAGAGT AAACTCCCTG CCAGTGAATC CCAGAGTTGG TGAGGATGAA 
AACTCCAACT CAGCCCTGCC CCCATGGTCT CACCATGGCC AGGCCTCGGG GGGAAGCTAT 
GGACTCAAGT ATGGCCTCAC TACATATAAA ATCGTTCCTC CCAAACCAGA GATGAGGTGT 
TATGACAGGG ATGTGTCCCT CTCCACTGGT GCCATTAAGA TTGATGAACT AGGGAATCTG 
ATGAGTCCTC ACATGAATGG AAGCAGGACC ATATCCAAGC CATCAGCTGT TGCCGAAACA 
GAGGCTCCAC CTATTGGAAA AGTCAAAGAG TTCTGGAGGC GCAACTCGAT GGAAAAGTAC 
CTGAATGGGC CAGCCGAGTG CACGGTCAAA AAGGCCCCCT CCACCACCAT CACTGCCACA 
TCCGAAAAAC CACAGCGAGA TGAGACGAAG GCAGGCTTTA CGCTGACCAC TCCGGAGCAG 
CAGCCTGCCT CACAGGAATA CGGGGCACCC CCGGAGGAAG ACAGAAGCCG GCCACATTCA 
GCCGTCTCCT GTCCTGTGAA AGTGCCAGCA CCTAATCCCA CAGACATCAC ATTCCTCAAG 
CCTCAGAGAA GAACATCCAG CCAGTATGTG GCTTCTGCTA TTGCCAAGAA GATGGGACCT 
CCAAAAGTCC ATGCTGATGT GGTGAGACCA CATAAGAAGA CTGCCGAACA AGGCCACGAG 
GAAGCAAAGC TCGCTAGACC ACCTCCAGCC TGGAAAGACA GTGCAGTCCC CAACCTGTGT 
TCAGAGGCCG GCCAGTGTGA GCATGGCACA AACCAGGGCT CTGTCCGCCT TCCCAGCAAC 
CCTGGTGGGC AGTTGCCAGC GGACCATCCC AAAGTAGAGG TCAACAGCAC ATATGGGAAG 
TCAGCGACTC ACAACTCTCC TGCTGCTGTC CACAGAAACT CCTATTTCCT CCCAGGCAGA 
TCTTCCCAGC GGGATCGTGT TTCTGTAGGA CAGAGCTGTG GTTTCCATGA AAAGCAAACT 
ATAAGTAACC AAAAGATGAA TTCAACATCT AACCCCTCGC AGGCACTGGA CAAGGCACAC 
CCAGCCCCCC TGCTCTTGAC AGAAGCCCGT GACTCTGGGA GGATACTGGT GAATGGCTCT 
GCACAGACCC CAGGAAATTG TGAGCCTCCC CACTCTCAGA AAGAGTCCAC CCTGACTAGC 
TATATTATCT TACAGACAGA GGAAAAGCCC AGTCCGTTAT CTGCAGATGG TCAAAACTCA 
GATGATGCTC TACCGTCGAG CATTTTTGGG CCAAAGAAAA AATTCAAGCC TGTCGTTCAA 
AGGCCACTTC CAAAAGATAT ATCCCTGCAC AGTGCCCTGA TGGAAGCCAT CCACACCTCA 
GGAGGGAGGG ACAAGCTCCG AAAGACTGCA GAACAGGCCT CAGAAGGAAG GCCAAAGAAA 
CCATCCTACG TGGAGGCGGA GAGCGAGCGA TCTGCCCTTC TGGCAGCCAT CCGGGGCCAC 
AGTGGGACTC TCAGCCTGAG GAAGGTGTCA TCCCTGGCCT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCTG CACTCATGGC TCCAGGTGTG GACAAGCCTC AGCAAGAAGA CCGTGGCCTT 
CCACCCCCAC CTGCCCTGCC ACCACCATCC ACTCCAGCAT CCCAGGTTCC CTCAGCCTCC 
ATACCGGTTT CTAGGTTCAG CATCGGCACC CTCAGCAACC CAGTAAATGC TAGGCAAGCC 
TTGATGGATG CTATCCGCTC GGGCACAGGA GCTGCAAGAC TCAGAAAGGT TCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cobl ( XM_017599226.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454715.1 Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa58313
Clone ID Related Accession (Same CDS sequence)	XM_017599226.1
Accession Version	XM_017599226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein cordon-bleu isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGAAA TGCTCAGGAA ATCATCCCTT 
GGCAATGATG AGACAGATAA GGAGAAGAAA AAATTTCTTG GGTTTTTCAA AGTTAATAAG 
AGGAGCAACA GTAAGGCTGA GCATCTTGGG CTGTCGGGCG CAGATAGCGA CGAGGATCCC 
TCGAAGTCAG CGTCTGGAGG GGACTTGAAC GGCTGTGTAA CAACACCCAA CTCACCATCC 
TTGCATTCTC GGTCCCTGAC ACTGGGTCCC TCCCTCTCAC TGGGCAACAT CTCTGGAATG 
TCTATGAAGT CAGACATGAA GAAGCGCAGA GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG 
GGTCAAGACA AGGTATCAGA AAAGGCCTCC CTCAGCTCGC AGGCCGATCT TCAGAAGAAG 
AAGAGGCGGG CGCCAGCTCC TCCTCCACCA CAGCCGCCAC CACCAAGTCC TGTGGTTCCC 
AACCGCAAGG AAGATAAGGA AGAGAACAGG AAGAGCACAG TGGGTGTTGG ACGTCAGGTG 
CCACAAAAGC CTCCCAGAGG CACAGCTCGG GGCCCACCCC AGTTAGTGCT CCCCCCGCCC 
CCACCCTATC CTCCTCCTGA CACAGATGTA ACAGAGCCTG TCACCTTTCC TGGGGAAGGC 
GCTGGTTCCG AGACCTCAGA GCTGAGACCT AAACTGAGTC TGCCTCTGGG GCCTGGCAGC 
CACTGCAGCA TGGGTGGAGT CTCACAGGTG CCCGCGGAGG CTGAGGAGAC GGCATCTGTG 
GGCAGCTGTT TTGCATCGGA GGACACAACC GAAGACTCAG GGGTCATGAG CTCTCCCTCA 
GATGTCATCT CCCTGGACTC ACAACAAGAT AGCATGAGAT CTAAGGATAA ATGGTCCACA 
GACCAGGAGG ACTGCAGTGA CCAGGACCTG GCTGGAACAC CAGAACTGGG TCCCCAAAAG 
AGCCCATCAT GGGGGAAACA TGGCTCAGGA AGCTCAATTT TAAGGACTGA GAAAGCCGCC 
ATGCCTGGGA ATGATGATGA AGATCTGTCC ATCACGGGAC TCTTCCATCA GACTCGAGCA 
GAGCTTGACA AAGACCTGGG AGGGATGGAA GAAAATTATG AAACAGATAC CAGCTCTCTA 
ACCAGCTCTG TAAATGGGGT ATCCAACCAC AGTCTGCAAG AAGCCATAAT CCCTGATAGT 
GGTGTGGACG ACATCCCTGT CACTTTTATC GGGGAAGTTT CTGACGAGCC TTTCGACTCG 
GGGCTGTTCT TCAGTGGAAG TAACAATGCT GCTGCTTTGA ACCAAGGGGG CATTGCCAGC 
CAAAGATCCC ACCTGCCCCC ATATCAGACT GAGCAGAGCC AGCCATTCAT AAGGACAAAC 
AGAAAAGAGC CTGACCCCTC CCTTCCTTCC CAAGATTACA GAAAGAGAAA TCAGCCAATC 
TTAGCCAACA CATCTGAAAA TGAGAATCCA GTTGGAATAG ACCCCAGAGT TACATCATTT 
GTTTCAAAGC CTTCGACAGA TGACCCAAAG GCAAAGGATA AAGACAAGAT GTGCGGTTCT 
GGTCCCAGTG AGAAGACACA GACCGGTCAC AGAGTAAACT CCCTGCCAGT GAATCCCAGA 
GTTGGTGAGG ATGAAAACTC CAACTCAGCC CTGCCCCCAT GGTCTCACCA TGGCCAGGCC 
TCGGGGGGAA GCTATGGACT CAAGTATGGC CTCACTACAT ATAAAATCGT TCCTCCCAAA 
CCAGAGATGA GGTGTTATGA CAGGGATGTG TCCCTCTCCA CTGGTGCCAT TAAGATTGAT 
GAACTAGGGA ATCTGATGAG TCCTCACATG AATGGAAGCA GGACCATATC CAAGCCATCA 
GCTGTTGCCG AAACAGAGGC TCCACCTATT GGAAAAGTCA AAGAGTTCTG GAGGCGCAAC 
TCGATGGAAA AGTACCTGAA TGGGCCAGCC GAGTGCACGG TCAAAAAGGC CCCCTCCACC 
ACCATCACTG CCACATCCGA AAAACCACAG CGAGATGAGA CGAAGGCAGG CTTTACGCTG 
ACCACTCCGG AGCAGCAGCC TGCCTCACAG GAATACGGGG CACCCCCGGA GGAAGACAGA 
AGCCGGCCAC ATTCAGCCGT CTCCTGTCCT GTGAAAGTGC CAGCACCTAA TCCCACAGAC 
ATCACATTCC TCAAGCCTCA GAGAAGAACA TCCAGCCAGT ATGTGGCTTC TGCTATTGCC 
AAGAAGATGG GACCTCCAAA AGTCCATGCT GATGTGGTGA GACCACATAA GAAGACTGCC 
GAACAAGGCC ACGAGGAAGC AAAGCTCGCT AGACCACCTC CAGCCTGGAA AGACAGTGCA 
GTCCCCAACC TGTGTTCAGA GGCCGGCCAG TGTGAGCATG GCACAAACCA GGGCTCTGTC 
CGCCTTCCCA GCAACCCTGG TGGGCAGTTG CCAGCGGACC ATCCCAAAGT AGAGGTCAAC 
AGCACATATG GGAAGTCAGC GACTCACAAC TCTCCTGCTG CTGTCCACAG AAACTCCTAT 
TTCCTCCCAG GCAGATCTTC CCAGCGGGAT CGTGTTTCTG TAGGACAGAG CTGTGGTTTC 
CATGAAAAGC AAACTATAAG TAACCAAAAG ATGAATTCAA CATCTAACCC CTCGCAGGCA 
CTGGACAAGG CACACCCAGC CCCCCTGCTC TTGACAGAAG CCCGTGACTC TGGGAGGATA 
CTGGTGAATG GCTCTGCACA GACCCCAGGA AATTGTGAGC CTCCCCACTC TCAGAAAGAG 
TCCACCCTGA CTAGCTATAT TATCTTACAG ACAGAGGAAA AGCCCAGTCC GTTATCTGCA 
GATGGTCAAA ACTCAGATGA TGCTCTACCG TCGAGCATTT TTGGGCCAAA GAAAAAATTC 
AAGCCTGTCG TTCAAAGGCC ACTTCCAAAA GATATATCCC TGCACAGTGC CCTGATGGAA 
GCCATCCACA CCTCAGGAGG GAGGGACAAG CTCCGAAAGA CTGCAGAACA GGCCTCAGAA 
GGAAGGCCAA AGAAACCATC CTACGTGGAG GCGGAGAGCG AGCGATCTGC CCTTCTGGCA 
GCCATCCGGG GCCACAGTGG GACTCTCAGC CTGAGGAAGG TGTCATCCCT GGCCTCTGAG 
GAGCTCCAGA GCTTCCGAGA TGCTGCACTC ATGGCTCCAG GTGTGGACAA GCCTCAGCAA 
GAAGACCGTG GCCTTCCACC CCCACCTGCC CTGCCACCAC CATCCACTCC AGCATCCCAG 
GTTCCCTCAG CCTCCATACC GGTTTCTAGG TTCAGCATCG GCACCCTCAG CAACCCAGTA 
AATGCTAGGC AAGCCTTGAT GGATGCTATC CGCTCGGGCA CAGGAGCTGC AAGACTCAGA 
AAGGTTCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454715.1
CDS	1282..5262
Translation	MKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVHLKEALHNSTLDITVVLPSGLEKQSVVSGSRAVMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNAHTVFLKEKVPEEKGKPGLTKAPEKSVRLVVNYLRTQKAVMRVSPEVPLQNILPVICAKCEVSPDHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRREMLRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEHLGLSGADSDEDPSKSASGGDLNGCVTTPNSPSLHSRSLTLGPSLSLGNISGMSMKSDMKKRRAPPPPSPGLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQPPPPSPVVPNRKEDKEENRKSTVGVGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVTEPVTFPGEGAGSETSELRPKLSLPLGPGSHCSMGGVSQVPAEAEETASVGSCFASEDTTEDSGVMSSPSDVISLDSQQDSMRSKDKWSTDQEDCSDQDLAGTPELGPQKSPSWGKHGSGSSILRTEKAAMPGNDDEDLSITGLFHQTRAELDKDLGGMEENYETDTSSLTSSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFFSGSNNAAALNQGGIASQRSHLPPYQTEQSQPFIRTNRKEPDPSLPSQDYRKRNQPILANTSENENPVGIDPRVTSFVSKPSTDDPKAKDKDKMCGSGPSEKTQTGHRVNSLPVNPRVGEDENSNSALPPWSHHGQASGGSYGLKYGLTTYKIVPPKPEMRCYDRDVSLSTGAIKIDELGNLMSPHMNGSRTISKPSAVAETEAPPIGKVKEFWRRNSMEKYLNGPAECTVKKAPSTTITATSEKPQRDETKAGFTLTTPEQQPASQEYGAPPEEDRSRPHSAVSCPVKVPAPNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKKTAEQGHEEAKLARPPPAWKDSAVPNLCSEAGQCEHGTNQGSVRLPSNPGGQLPADHPKVEVNSTYGKSATHNSPAAVHRNSYFLPGRSSQRDRVSVGQSCGFHEKQTISNQKMNSTSNPSQALDKAHPAPLLLTEARDSGRILVNGSAQTPGNCEPPHSQKESTLTSYIILQTEEKPSPLSADGQNSDDALPSSIFGPKKKFKPVVQRPLPKDISLHSALMEAIHTSGGRDKLRKTAEQASEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRDAALMAPGVDKPQQEDRGLPPPPALPPPSTPASQVPSASIPVSRFSIGTLSNPVNARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_017599226.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599226.1

ORF Insert Sequence:

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGAAA TGCTCAGGAA ATCATCCCTT 
GGCAATGATG AGACAGATAA GGAGAAGAAA AAATTTCTTG GGTTTTTCAA AGTTAATAAG 
AGGAGCAACA GTAAGGCTGA GCATCTTGGG CTGTCGGGCG CAGATAGCGA CGAGGATCCC 
TCGAAGTCAG CGTCTGGAGG GGACTTGAAC GGCTGTGTAA CAACACCCAA CTCACCATCC 
TTGCATTCTC GGTCCCTGAC ACTGGGTCCC TCCCTCTCAC TGGGCAACAT CTCTGGAATG 
TCTATGAAGT CAGACATGAA GAAGCGCAGA GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG 
GGTCAAGACA AGGTATCAGA AAAGGCCTCC CTCAGCTCGC AGGCCGATCT TCAGAAGAAG 
AAGAGGCGGG CGCCAGCTCC TCCTCCACCA CAGCCGCCAC CACCAAGTCC TGTGGTTCCC 
AACCGCAAGG AAGATAAGGA AGAGAACAGG AAGAGCACAG TGGGTGTTGG ACGTCAGGTG 
CCACAAAAGC CTCCCAGAGG CACAGCTCGG GGCCCACCCC AGTTAGTGCT CCCCCCGCCC 
CCACCCTATC CTCCTCCTGA CACAGATGTA ACAGAGCCTG TCACCTTTCC TGGGGAAGGC 
GCTGGTTCCG AGACCTCAGA GCTGAGACCT AAACTGAGTC TGCCTCTGGG GCCTGGCAGC 
CACTGCAGCA TGGGTGGAGT CTCACAGGTG CCCGCGGAGG CTGAGGAGAC GGCATCTGTG 
GGCAGCTGTT TTGCATCGGA GGACACAACC GAAGACTCAG GGGTCATGAG CTCTCCCTCA 
GATGTCATCT CCCTGGACTC ACAACAAGAT AGCATGAGAT CTAAGGATAA ATGGTCCACA 
GACCAGGAGG ACTGCAGTGA CCAGGACCTG GCTGGAACAC CAGAACTGGG TCCCCAAAAG 
AGCCCATCAT GGGGGAAACA TGGCTCAGGA AGCTCAATTT TAAGGACTGA GAAAGCCGCC 
ATGCCTGGGA ATGATGATGA AGATCTGTCC ATCACGGGAC TCTTCCATCA GACTCGAGCA 
GAGCTTGACA AAGACCTGGG AGGGATGGAA GAAAATTATG AAACAGATAC CAGCTCTCTA 
ACCAGCTCTG TAAATGGGGT ATCCAACCAC AGTCTGCAAG AAGCCATAAT CCCTGATAGT 
GGTGTGGACG ACATCCCTGT CACTTTTATC GGGGAAGTTT CTGACGAGCC TTTCGACTCG 
GGGCTGTTCT TCAGTGGAAG TAACAATGCT GCTGCTTTGA ACCAAGGGGG CATTGCCAGC 
CAAAGATCCC ACCTGCCCCC ATATCAGACT GAGCAGAGCC AGCCATTCAT AAGGACAAAC 
AGAAAAGAGC CTGACCCCTC CCTTCCTTCC CAAGATTACA GAAAGAGAAA TCAGCCAATC 
TTAGCCAACA CATCTGAAAA TGAGAATCCA GTTGGAATAG ACCCCAGAGT TACATCATTT 
GTTTCAAAGC CTTCGACAGA TGACCCAAAG GCAAAGGATA AAGACAAGAT GTGCGGTTCT 
GGTCCCAGTG AGAAGACACA GACCGGTCAC AGAGTAAACT CCCTGCCAGT GAATCCCAGA 
GTTGGTGAGG ATGAAAACTC CAACTCAGCC CTGCCCCCAT GGTCTCACCA TGGCCAGGCC 
TCGGGGGGAA GCTATGGACT CAAGTATGGC CTCACTACAT ATAAAATCGT TCCTCCCAAA 
CCAGAGATGA GGTGTTATGA CAGGGATGTG TCCCTCTCCA CTGGTGCCAT TAAGATTGAT 
GAACTAGGGA ATCTGATGAG TCCTCACATG AATGGAAGCA GGACCATATC CAAGCCATCA 
GCTGTTGCCG AAACAGAGGC TCCACCTATT GGAAAAGTCA AAGAGTTCTG GAGGCGCAAC 
TCGATGGAAA AGTACCTGAA TGGGCCAGCC GAGTGCACGG TCAAAAAGGC CCCCTCCACC 
ACCATCACTG CCACATCCGA AAAACCACAG CGAGATGAGA CGAAGGCAGG CTTTACGCTG 
ACCACTCCGG AGCAGCAGCC TGCCTCACAG GAATACGGGG CACCCCCGGA GGAAGACAGA 
AGCCGGCCAC ATTCAGCCGT CTCCTGTCCT GTGAAAGTGC CAGCACCTAA TCCCACAGAC 
ATCACATTCC TCAAGCCTCA GAGAAGAACA TCCAGCCAGT ATGTGGCTTC TGCTATTGCC 
AAGAAGATGG GACCTCCAAA AGTCCATGCT GATGTGGTGA GACCACATAA GAAGACTGCC 
GAACAAGGCC ACGAGGAAGC AAAGCTCGCT AGACCACCTC CAGCCTGGAA AGACAGTGCA 
GTCCCCAACC TGTGTTCAGA GGCCGGCCAG TGTGAGCATG GCACAAACCA GGGCTCTGTC 
CGCCTTCCCA GCAACCCTGG TGGGCAGTTG CCAGCGGACC ATCCCAAAGT AGAGGTCAAC 
AGCACATATG GGAAGTCAGC GACTCACAAC TCTCCTGCTG CTGTCCACAG AAACTCCTAT 
TTCCTCCCAG GCAGATCTTC CCAGCGGGAT CGTGTTTCTG TAGGACAGAG CTGTGGTTTC 
CATGAAAAGC AAACTATAAG TAACCAAAAG ATGAATTCAA CATCTAACCC CTCGCAGGCA 
CTGGACAAGG CACACCCAGC CCCCCTGCTC TTGACAGAAG CCCGTGACTC TGGGAGGATA 
CTGGTGAATG GCTCTGCACA GACCCCAGGA AATTGTGAGC CTCCCCACTC TCAGAAAGAG 
TCCACCCTGA CTAGCTATAT TATCTTACAG ACAGAGGAAA AGCCCAGTCC GTTATCTGCA 
GATGGTCAAA ACTCAGATGA TGCTCTACCG TCGAGCATTT TTGGGCCAAA GAAAAAATTC 
AAGCCTGTCG TTCAAAGGCC ACTTCCAAAA GATATATCCC TGCACAGTGC CCTGATGGAA 
GCCATCCACA CCTCAGGAGG GAGGGACAAG CTCCGAAAGA CTGCAGAACA GGCCTCAGAA 
GGAAGGCCAA AGAAACCATC CTACGTGGAG GCGGAGAGCG AGCGATCTGC CCTTCTGGCA 
GCCATCCGGG GCCACAGTGG GACTCTCAGC CTGAGGAAGG TGTCATCCCT GGCCTCTGAG 
GAGCTCCAGA GCTTCCGAGA TGCTGCACTC ATGGCTCCAG GTGTGGACAA GCCTCAGCAA 
GAAGACCGTG GCCTTCCACC CCCACCTGCC CTGCCACCAC CATCCACTCC AGCATCCCAG 
GTTCCCTCAG CCTCCATACC GGTTTCTAGG TTCAGCATCG GCACCCTCAG CAACCCAGTA 
AATGCTAGGC AAGCCTTGAT GGATGCTATC CGCTCGGGCA CAGGAGCTGC AAGACTCAGA 
AAGGTTCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cobl ( XM_017599227.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454716.1 Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X3, mRNA.

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CloneID	ORa58314
Clone ID Related Accession (Same CDS sequence)	XM_017599227.1
Accession Version	XM_017599227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein cordon-bleu isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GGCTGTGTAA CAACACCCAA CTCACCATCC TTGCATTCTC GGTCCCTGAC ACTGGGTCCC 
TCCCTCTCAC TGGGCAACAT CTCTGGAATG TCTATGAAGT CAGACATGAA GAAGCGCAGA 
GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG GGTCAAGACA AGGTATCAGA AAAGGCCTCC 
CTCAGCTCGC AGGCCGATCT TCAGAAGAAG AAGAGGCGGG CGCCAGCTCC TCCTCCACCA 
CAGCCGCCAC CACCAAGTCC TGTGGTTCCC AACCGCAAGG AAGATAAGGA AGAGAACAGG 
AAGAGCACAG TGGGTGTTGG ACGTCAGGTG CCACAAAAGC CTCCCAGAGG CACAGCTCGG 
GGCCCACCCC AGTTAGTGCT CCCCCCGCCC CCACCCTATC CTCCTCCTGA CACAGATGTA 
ACAGAGCCTG TCACCTTTCC TGGGGAAGGC GCTGGTTCCG AGACCTCAGA GCTGAGACCT 
AAACTGAGTC TGCCTCTGGG GCCTGGCAGC CACTGCAGCA TGGGTGGAGT CTCACAGGTG 
CCCGCGGAGG CTGAGGAGAC GGCATCTGTG GGCAGCTGTT TTGCATCGGA GGACACAACC 
GAAGACTCAG GGGTCATGAG CTCTCCCTCA GATGTCATCT CCCTGGACTC ACAACAAGAT 
AGCATGAGAT CTAAGGATAA ATGGTCCACA GACCAGGAGG ACTGCAGTGA CCAGGACCTG 
GCTGGAACAC CAGAACTGGG TCCCCAAAAG AGCCCATCAT GGGGGAAACA TGGCTCAGGA 
AGCTCAATTT TAAGGACTGA GAAAGCCGCC ATGCCTGGGA ATGATGATGA AGATCTGTCC 
ATCACGGGAC TCTTCCATCA GACTCGAGCA GAGCTTGACA AAGACCTGGG AGGGATGGAA 
GAAAATTATG AAACAGATAC CAGCTCTCTA ACCAGCTCTG TAAATGGGGT ATCCAACCAC 
AGTCTGCAAG AAGCCATAAT CCCTGATAGT GGTGTGGACG ACATCCCTGT CACTTTTATC 
GGGGAAGTTT CTGACGAGCC TTTCGACTCG GGGCTGTTCT TCAGTGGAAG TAACAATGCT 
GCTGCTTTGA ACCAAGGGGG CATTGCCAGC CAAAGATCCC ACCTGCCCCC ATATCAGACT 
GAGCAGAGCC AGCCATTCAT AAGGACAAAC AGAAAAGAGC CTGACCCCTC CCTTCCTTCC 
CAAGATTACA GAAAGAGAAA TCAGCCAATC TTAGCCAACA CATCTGAAAA TGAGAATCCA 
GTTGGAATAG ACCCCAGAGT TACATCATTT GTTTCAAAGC CTTCGACAGA TGACCCAAAG 
GCAAAGGATA AAGACAAGAT GTGCGGTTCT GGTCCCAGTG AGAAGACACA GACCGGTCAC 
AGAGTAAACT CCCTGCCAGT GAATCCCAGA GTTGGTGAGG ATGAAAACTC CAACTCAGCC 
CTGCCCCCAT GGTCTCACCA TGGCCAGGCC TCGGGGGGAA GCTATGGACT CAAGTATGGC 
CTCACTACAT ATAAAATCGT TCCTCCCAAA CCAGAGATGA GGTGTTATGA CAGGGATGTG 
TCCCTCTCCA CTGGTGCCAT TAAGATTGAT GAACTAGGGA ATCTGATGAG TCCTCACATG 
AATGGAAGCA GGACCATATC CAAGCCATCA GCTGTTGCCG AAACAGAGGC TCCACCTATT 
GGAAAAGTCA AAGAGTTCTG GAGGCGCAAC TCGATGGAAA AGTACCTGAA TGGGCCAGCC 
GAGTGCACGG TCAAAAAGGC CCCCTCCACC ACCATCACTG CCACATCCGA AAAACCACAG 
CGAGATGAGA CGAAGGCAGG CTTTACGCTG ACCACTCCGG AGCAGCAGCC TGCCTCACAG 
GAATACGGGG CACCCCCGGA GGAAGACAGA AGCCGGCCAC ATTCAGCCGT CTCCTGTCCT 
GTGAAAGTGC CAGCACCTAA TCCCACAGAC ATCACATTCC TCAAGCCTCA GAGAAGAACA 
TCCAGCCAGT ATGTGGCTTC TGCTATTGCC AAGAAGATGG GACCTCCAAA AGTCCATGCT 
GATGTGGTGA GACCACATAA GAAGACTGCC GAACAAGGCC ACGAGGAAGC AAAGCTCGCT 
AGACCACCTC CAGCCTGGAA AGACAGTGCA GTCCCCAACC TGTGTTCAGA GGCCGGCCAG 
TGTGAGCATG GCACAAACCA GGGCTCTGTC CGCCTTCCCA GCAACCCTGG TGGGCAGTTG 
CCAGCGGACC ATCCCAAAGT AGAGGTCAAC AGCACATATG GGAAGTCAGC GACTCACAAC 
TCTCCTGCTG CTGTCCACAG AAACTCCTAT TTCCTCCCAG GCAGATCTTC CCAGCGGGAT 
CGTGTTTCTG TAGGACAGAG CTGTGGTTTC CATGAAAAGC AAACTATAAG TAACCAAAAG 
ATGAATTCAA CATCTAACCC CTCGCAGGCA CTGGACAAGG CACACCCAGC CCCCCTGCTC 
TTGACAGAAG CCCGTGACTC TGGGAGGATA CTGGTGAATG GCTCTGCACA GACCCCAGGA 
AATTGTGAGC CTCCCCACTC TCAGAAAGAG TCCACCCTGA CTAGCTATAT TATCTTACAG 
ACAGAGGAAA AGCCCAGTCC GTTATCTGCA GATGGTCAAA ACTCAGATGA TGCTCTACCG 
TCGAGCATTT TTGGGCCAAA GAAAAAATTC AAGCCTGTCG TTCAAAGGCC ACTTCCAAAA 
GATATATCCC TGCACAGTGC CCTGATGGAA GCCATCCACA CCTCAGGAGG GAGGGACAAG 
CTCCGAAAGA CTGCAGAACA GGCCTCAGAA GGAAGGCCAA AGAAACCATC CTACGTGGAG 
GCGGAGAGCG AGCGATCTGC CCTTCTGGCA GCCATCCGGG GCCACAGTGG GACTCTCAGC 
CTGAGGAAGG TGTCATCCCT GGCCTCTGAG GAGCTCCAGA GCTTCCGAGA TGCTGCACTC 
ATGGCTCCAG GTGTGGACAA GCCTCAGCAA GAAGACCGTG GCCTTCCACC CCCACCTGCC 
CTGCCACCAC CATCCACTCC AGCATCCCAG GTTCCCTCAG CCTCCATACC GGTTTCTAGG 
TTCAGCATCG GCACCCTCAG CAACCCAGTA AATGCTAGGC AAGCCTTGAT GGATGCTATC 
CGCTCGGGCA CAGGAGCTGC AAGACTCAGA AAGGTTCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454716.1
CDS	1283..5233
Translation	MKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVHLKEALHNSTLDITVVLPSGLEKQSVVSGSRAVMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNAHTVFLKEKVPEEKGKPGLTKAPEKSVRLVVNYLRTQKAVMRVSPEVPLQNILPVICAKCEVSPDHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVLLTKTQSEPSLSCQEMLRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCVTTPNSPSLHSRSLTLGPSLSLGNISGMSMKSDMKKRRAPPPPSPGLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQPPPPSPVVPNRKEDKEENRKSTVGVGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVTEPVTFPGEGAGSETSELRPKLSLPLGPGSHCSMGGVSQVPAEAEETASVGSCFASEDTTEDSGVMSSPSDVISLDSQQDSMRSKDKWSTDQEDCSDQDLAGTPELGPQKSPSWGKHGSGSSILRTEKAAMPGNDDEDLSITGLFHQTRAELDKDLGGMEENYETDTSSLTSSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFFSGSNNAAALNQGGIASQRSHLPPYQTEQSQPFIRTNRKEPDPSLPSQDYRKRNQPILANTSENENPVGIDPRVTSFVSKPSTDDPKAKDKDKMCGSGPSEKTQTGHRVNSLPVNPRVGEDENSNSALPPWSHHGQASGGSYGLKYGLTTYKIVPPKPEMRCYDRDVSLSTGAIKIDELGNLMSPHMNGSRTISKPSAVAETEAPPIGKVKEFWRRNSMEKYLNGPAECTVKKAPSTTITATSEKPQRDETKAGFTLTTPEQQPASQEYGAPPEEDRSRPHSAVSCPVKVPAPNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKKTAEQGHEEAKLARPPPAWKDSAVPNLCSEAGQCEHGTNQGSVRLPSNPGGQLPADHPKVEVNSTYGKSATHNSPAAVHRNSYFLPGRSSQRDRVSVGQSCGFHEKQTISNQKMNSTSNPSQALDKAHPAPLLLTEARDSGRILVNGSAQTPGNCEPPHSQKESTLTSYIILQTEEKPSPLSADGQNSDDALPSSIFGPKKKFKPVVQRPLPKDISLHSALMEAIHTSGGRDKLRKTAEQASEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRDAALMAPGVDKPQQEDRGLPPPPALPPPSTPASQVPSASIPVSRFSIGTLSNPVNARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_017599227.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599227.1

ORF Insert Sequence:

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GGCTGTGTAA CAACACCCAA CTCACCATCC TTGCATTCTC GGTCCCTGAC ACTGGGTCCC 
TCCCTCTCAC TGGGCAACAT CTCTGGAATG TCTATGAAGT CAGACATGAA GAAGCGCAGA 
GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG GGTCAAGACA AGGTATCAGA AAAGGCCTCC 
CTCAGCTCGC AGGCCGATCT TCAGAAGAAG AAGAGGCGGG CGCCAGCTCC TCCTCCACCA 
CAGCCGCCAC CACCAAGTCC TGTGGTTCCC AACCGCAAGG AAGATAAGGA AGAGAACAGG 
AAGAGCACAG TGGGTGTTGG ACGTCAGGTG CCACAAAAGC CTCCCAGAGG CACAGCTCGG 
GGCCCACCCC AGTTAGTGCT CCCCCCGCCC CCACCCTATC CTCCTCCTGA CACAGATGTA 
ACAGAGCCTG TCACCTTTCC TGGGGAAGGC GCTGGTTCCG AGACCTCAGA GCTGAGACCT 
AAACTGAGTC TGCCTCTGGG GCCTGGCAGC CACTGCAGCA TGGGTGGAGT CTCACAGGTG 
CCCGCGGAGG CTGAGGAGAC GGCATCTGTG GGCAGCTGTT TTGCATCGGA GGACACAACC 
GAAGACTCAG GGGTCATGAG CTCTCCCTCA GATGTCATCT CCCTGGACTC ACAACAAGAT 
AGCATGAGAT CTAAGGATAA ATGGTCCACA GACCAGGAGG ACTGCAGTGA CCAGGACCTG 
GCTGGAACAC CAGAACTGGG TCCCCAAAAG AGCCCATCAT GGGGGAAACA TGGCTCAGGA 
AGCTCAATTT TAAGGACTGA GAAAGCCGCC ATGCCTGGGA ATGATGATGA AGATCTGTCC 
ATCACGGGAC TCTTCCATCA GACTCGAGCA GAGCTTGACA AAGACCTGGG AGGGATGGAA 
GAAAATTATG AAACAGATAC CAGCTCTCTA ACCAGCTCTG TAAATGGGGT ATCCAACCAC 
AGTCTGCAAG AAGCCATAAT CCCTGATAGT GGTGTGGACG ACATCCCTGT CACTTTTATC 
GGGGAAGTTT CTGACGAGCC TTTCGACTCG GGGCTGTTCT TCAGTGGAAG TAACAATGCT 
GCTGCTTTGA ACCAAGGGGG CATTGCCAGC CAAAGATCCC ACCTGCCCCC ATATCAGACT 
GAGCAGAGCC AGCCATTCAT AAGGACAAAC AGAAAAGAGC CTGACCCCTC CCTTCCTTCC 
CAAGATTACA GAAAGAGAAA TCAGCCAATC TTAGCCAACA CATCTGAAAA TGAGAATCCA 
GTTGGAATAG ACCCCAGAGT TACATCATTT GTTTCAAAGC CTTCGACAGA TGACCCAAAG 
GCAAAGGATA AAGACAAGAT GTGCGGTTCT GGTCCCAGTG AGAAGACACA GACCGGTCAC 
AGAGTAAACT CCCTGCCAGT GAATCCCAGA GTTGGTGAGG ATGAAAACTC CAACTCAGCC 
CTGCCCCCAT GGTCTCACCA TGGCCAGGCC TCGGGGGGAA GCTATGGACT CAAGTATGGC 
CTCACTACAT ATAAAATCGT TCCTCCCAAA CCAGAGATGA GGTGTTATGA CAGGGATGTG 
TCCCTCTCCA CTGGTGCCAT TAAGATTGAT GAACTAGGGA ATCTGATGAG TCCTCACATG 
AATGGAAGCA GGACCATATC CAAGCCATCA GCTGTTGCCG AAACAGAGGC TCCACCTATT 
GGAAAAGTCA AAGAGTTCTG GAGGCGCAAC TCGATGGAAA AGTACCTGAA TGGGCCAGCC 
GAGTGCACGG TCAAAAAGGC CCCCTCCACC ACCATCACTG CCACATCCGA AAAACCACAG 
CGAGATGAGA CGAAGGCAGG CTTTACGCTG ACCACTCCGG AGCAGCAGCC TGCCTCACAG 
GAATACGGGG CACCCCCGGA GGAAGACAGA AGCCGGCCAC ATTCAGCCGT CTCCTGTCCT 
GTGAAAGTGC CAGCACCTAA TCCCACAGAC ATCACATTCC TCAAGCCTCA GAGAAGAACA 
TCCAGCCAGT ATGTGGCTTC TGCTATTGCC AAGAAGATGG GACCTCCAAA AGTCCATGCT 
GATGTGGTGA GACCACATAA GAAGACTGCC GAACAAGGCC ACGAGGAAGC AAAGCTCGCT 
AGACCACCTC CAGCCTGGAA AGACAGTGCA GTCCCCAACC TGTGTTCAGA GGCCGGCCAG 
TGTGAGCATG GCACAAACCA GGGCTCTGTC CGCCTTCCCA GCAACCCTGG TGGGCAGTTG 
CCAGCGGACC ATCCCAAAGT AGAGGTCAAC AGCACATATG GGAAGTCAGC GACTCACAAC 
TCTCCTGCTG CTGTCCACAG AAACTCCTAT TTCCTCCCAG GCAGATCTTC CCAGCGGGAT 
CGTGTTTCTG TAGGACAGAG CTGTGGTTTC CATGAAAAGC AAACTATAAG TAACCAAAAG 
ATGAATTCAA CATCTAACCC CTCGCAGGCA CTGGACAAGG CACACCCAGC CCCCCTGCTC 
TTGACAGAAG CCCGTGACTC TGGGAGGATA CTGGTGAATG GCTCTGCACA GACCCCAGGA 
AATTGTGAGC CTCCCCACTC TCAGAAAGAG TCCACCCTGA CTAGCTATAT TATCTTACAG 
ACAGAGGAAA AGCCCAGTCC GTTATCTGCA GATGGTCAAA ACTCAGATGA TGCTCTACCG 
TCGAGCATTT TTGGGCCAAA GAAAAAATTC AAGCCTGTCG TTCAAAGGCC ACTTCCAAAA 
GATATATCCC TGCACAGTGC CCTGATGGAA GCCATCCACA CCTCAGGAGG GAGGGACAAG 
CTCCGAAAGA CTGCAGAACA GGCCTCAGAA GGAAGGCCAA AGAAACCATC CTACGTGGAG 
GCGGAGAGCG AGCGATCTGC CCTTCTGGCA GCCATCCGGG GCCACAGTGG GACTCTCAGC 
CTGAGGAAGG TGTCATCCCT GGCCTCTGAG GAGCTCCAGA GCTTCCGAGA TGCTGCACTC 
ATGGCTCCAG GTGTGGACAA GCCTCAGCAA GAAGACCGTG GCCTTCCACC CCCACCTGCC 
CTGCCACCAC CATCCACTCC AGCATCCCAG GTTCCCTCAG CCTCCATACC GGTTTCTAGG 
TTCAGCATCG GCACCCTCAG CAACCCAGTA AATGCTAGGC AAGCCTTGAT GGATGCTATC 
CGCTCGGGCA CAGGAGCTGC AAGACTCAGA AAGGTTCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cobl ( XM_017599228.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454717.1 Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X4, mRNA.

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CloneID	ORa58315
Clone ID Related Accession (Same CDS sequence)	XM_017599228.1
Accession Version	XM_017599228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein cordon-bleu isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GCTGAGCATC TTGGGCTGTC GGGCGCAGAT AGCGACGAGG ATCCCTCGAA GTCAGCGTCT 
GGAGGGGACT TGAACGGCTG TGTAACAACA CCCAACTCAC CATCCTTGCA TTCTCGGTCC 
CTGACACTGG GTCCCTCCCT CTCACTGGGC AACATCTCTG GAATGTCTAT GAAGTCAGAC 
ATGAAGAAGC GCAGAGCCCC TCCTCCTCCA AGTCCCGGGC TGCTGGGTCA AGACAAGGTA 
TCAGAAAAGG CCTCCCTCAG CTCGCAGGCC GATCTTCAGA AGAAGAAGAG GCGGGCGCCA 
GCTCCTCCTC CACCACAGCC GCCACCACCA AGTCCTGTGG TTCCCAACCG CAAGGAAGAT 
AAGGAAGAGA ACAGGAAGAG CACAGTGGTG AGTCTGCCTC TGGGGCCTGG CAGCCACTGC 
AGCATGGGTG GAGTCTCACA GGTGCCCGCG GAGGCTGAGG AGACGGCATC TGTGGGCAGC 
TGTTTTGCAT CGGAGGACAC AACCGAAGAC TCAGGGGTCA TGAGCTCTCC CTCAGATGTC 
ATCTCCCTGG ACTCACAACA AGATAGCATG AGATCTAAGG ATAAATGGTC CACAGACCAG 
GAGGACTGCA GTGACCAGGA CCTGGCTGGA ACACCAGAAC TGGGTCCCCA AAAGAGCCCA 
TCATGGGGGA AACATGGCTC AGGAAGCTCA ATTTTAAGGA CTGAGAAAGC CGCCATGCCT 
GGGAATGATG ATGAAGATCT GTCCATCACG GGACTCTTCC ATCAGACTCG AGCAGAGCTT 
GACAAAGACC TGGGAGGGAT GGAAGAAAAT TATGAAACAG ATACCAGCTC TCTAACCAGC 
TCTGTAAATG GGGTATCCAA CCACAGTCTG CAAGAAGCCA TAATCCCTGA TAGTGGTGTG 
GACGACATCC CTGTCACTTT TATCGGGGAA GTTTCTGACG AGCCTTTCGA CTCGGGGCTG 
TTCTTCAGTG GAAGTAACAA TGCTGCTGCT TTGAACCAAG GGGGCATTGC CAGCCAAAGA 
TCCCACCTGC CCCCATATCA GACTGAGCAG AGCCAGCCAT TCATAAGGAC AAACAGAAAA 
GAGCCTGACC CCTCCCTTCC TTCCCAAGAT TACAGAAAGA GAAATCAGCC AATCTTAGCC 
AACACATCTG AAAATGAGAA TCCAGTTGGA ATAGACCCCA GAGTTACATC ATTTGTTTCA 
AAGCCTTCGA CAGATGACCC AAAGGCAAAG GATAAAGACA AGATGTGCGG TTCTGGTCCC 
AGTGAGAAGA CACAGACCGG TCACAGAGTA AACTCCCTGC CAGTGAATCC CAGAGTTGGT 
GAGGATGAAA ACTCCAACTC AGCCCTGCCC CCATGGTCTC ACCATGGCCA GGCCTCGGGG 
GGAAGCTATG GACTCAAGTA TGGCCTCACT ACATATAAAA TCGTTCCTCC CAAACCAGAG 
ATGAGGTGTT ATGACAGGGA TGTGTCCCTC TCCACTGGTG CCATTAAGAT TGATGAACTA 
GGGAATCTGA TGAGTCCTCA CATGAATGGA AGCAGGACCA TATCCAAGCC ATCAGCTGTT 
GCCGAAACAG AGGCTCCACC TATTGGAAAA GTCAAAGAGT TCTGGAGGCG CAACTCGATG 
GAAAAGTACC TGAATGGGCC AGCCGAGTGC ACGGTCAAAA AGGCCCCCTC CACCACCATC 
ACTGCCACAT CCGAAAAACC ACAGCGAGAT GAGACGAAGG CAGGCTTTAC GCTGACCACT 
CCGGAGCAGC AGCCTGCCTC ACAGGAATAC GGGGCACCCC CGGAGGAAGA CAGAAGCCGG 
CCACATTCAG CCGTCTCCTG TCCTGTGAAA GTGCCAGCAC CTAATCCCAC AGACATCACA 
TTCCTCAAGC CTCAGAGAAG AACATCCAGC CAGTATGTGG CTTCTGCTAT TGCCAAGAAG 
ATGGGACCTC CAAAAGTCCA TGCTGATGTG GTGAGACCAC ATAAGAAGAC TGCCGAACAA 
GGCCACGAGG AAGCAAAGCT CGCTAGACCA CCTCCAGCCT GGAAAGACAG TGCAGTCCCC 
AACCTGTGTT CAGAGGCCGG CCAGTGTGAG CATGGCACAA ACCAGGGCTC TGTCCGCCTT 
CCCAGCAACC CTGGTGGGCA GTTGCCAGCG GACCATCCCA AAGTAGAGGT CAACAGCACA 
TATGGGAAGT CAGCGACTCA CAACTCTCCT GCTGCTGTCC ACAGAAACTC CTATTTCCTC 
CCAGGCAGAT CTTCCCAGCG GGATCGTGTT TCTGTAGGAC AGAGCTGTGG TTTCCATGAA 
AAGCAAACTA TAAGTAACCA AAAGATGAAT TCAACATCTA ACCCCTCGCA GGCACTGGAC 
AAGGCACACC CAGCCCCCCT GCTCTTGACA GAAGCCCGTG ACTCTGGGAG GATACTGGTG 
AATGGCTCTG CACAGACCCC AGGAAATTGT GAGCCTCCCC ACTCTCAGAA AGAGTCCACC 
CTGACTAGCT ATATTATCTT ACAGACAGAG GAAAAGCCCA GTCCGTTATC TGCAGATGGT 
CAAAACTCAG ATGATGCTCT ACCGTCGAGC ATTTTTGGGC CAAAGAAAAA ATTCAAGCCT 
GTCGTTCAAA GGCCACTTCC AAAAGATATA TCCCTGCACA GTGCCCTGAT GGAAGCCATC 
CACACCTCAG GAGGGAGGGA CAAGCTCCGA AAGACTGCAG AACAGGCCTC AGAAGGAAGG 
CCAAAGAAAC CATCCTACGT GGAGGCGGAG AGCGAGCGAT CTGCCCTTCT GGCAGCCATC 
CGGGGCCACA GTGGGACTCT CAGCCTGAGG AAGGTGTCAT CCCTGGCCTC TGAGGAGCTC 
CAGAGCTTCC GAGATGCTGC ACTCATGGCT CCAGGTGTGG ACAAGCCTCA GCAAGAAGAC 
CGTGGCCTTC CACCCCCACC TGCCCTGCCA CCACCATCCA CTCCAGCATC CCAGGTTCCC 
TCAGCCTCCA TACCGGTTTC TAGGTTCAGC ATCGGCACCC TCAGCAACCC AGTAAATGCT 
AGGCAAGCCT TGATGGATGC TATCCGCTCG GGCACAGGAG CTGCAAGACT CAGAAAGGTT 
CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454717.1
CDS	1282..5136
Translation	MKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVHLKEALHNSTLDITVVLPSGLEKQSVVSGSRAVMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNAHTVFLKEKVPEEKGKPGLTKAPEKSVRLVVNYLRTQKAVMRVSPEVPLQNILPVICAKCEVSPDHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVLLTKTQSEPSLSCQEMLRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEHLGLSGADSDEDPSKSASGGDLNGCVTTPNSPSLHSRSLTLGPSLSLGNISGMSMKSDMKKRRAPPPPSPGLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQPPPPSPVVPNRKEDKEENRKSTVVSLPLGPGSHCSMGGVSQVPAEAEETASVGSCFASEDTTEDSGVMSSPSDVISLDSQQDSMRSKDKWSTDQEDCSDQDLAGTPELGPQKSPSWGKHGSGSSILRTEKAAMPGNDDEDLSITGLFHQTRAELDKDLGGMEENYETDTSSLTSSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFFSGSNNAAALNQGGIASQRSHLPPYQTEQSQPFIRTNRKEPDPSLPSQDYRKRNQPILANTSENENPVGIDPRVTSFVSKPSTDDPKAKDKDKMCGSGPSEKTQTGHRVNSLPVNPRVGEDENSNSALPPWSHHGQASGGSYGLKYGLTTYKIVPPKPEMRCYDRDVSLSTGAIKIDELGNLMSPHMNGSRTISKPSAVAETEAPPIGKVKEFWRRNSMEKYLNGPAECTVKKAPSTTITATSEKPQRDETKAGFTLTTPEQQPASQEYGAPPEEDRSRPHSAVSCPVKVPAPNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKKTAEQGHEEAKLARPPPAWKDSAVPNLCSEAGQCEHGTNQGSVRLPSNPGGQLPADHPKVEVNSTYGKSATHNSPAAVHRNSYFLPGRSSQRDRVSVGQSCGFHEKQTISNQKMNSTSNPSQALDKAHPAPLLLTEARDSGRILVNGSAQTPGNCEPPHSQKESTLTSYIILQTEEKPSPLSADGQNSDDALPSSIFGPKKKFKPVVQRPLPKDISLHSALMEAIHTSGGRDKLRKTAEQASEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRDAALMAPGVDKPQQEDRGLPPPPALPPPSTPASQVPSASIPVSRFSIGTLSNPVNARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_017599228.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599228.1

ORF Insert Sequence:

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGTTC TTCTGACCAA AACACAGTCT 
GAGCCTTCCC TGAGCTGTCA AGAAATGCTC AGGAAATCAT CCCTTGGCAA TGATGAGACA 
GATAAGGAGA AGAAAAAATT TCTTGGGTTT TTCAAAGTTA ATAAGAGGAG CAACAGTAAG 
GCTGAGCATC TTGGGCTGTC GGGCGCAGAT AGCGACGAGG ATCCCTCGAA GTCAGCGTCT 
GGAGGGGACT TGAACGGCTG TGTAACAACA CCCAACTCAC CATCCTTGCA TTCTCGGTCC 
CTGACACTGG GTCCCTCCCT CTCACTGGGC AACATCTCTG GAATGTCTAT GAAGTCAGAC 
ATGAAGAAGC GCAGAGCCCC TCCTCCTCCA AGTCCCGGGC TGCTGGGTCA AGACAAGGTA 
TCAGAAAAGG CCTCCCTCAG CTCGCAGGCC GATCTTCAGA AGAAGAAGAG GCGGGCGCCA 
GCTCCTCCTC CACCACAGCC GCCACCACCA AGTCCTGTGG TTCCCAACCG CAAGGAAGAT 
AAGGAAGAGA ACAGGAAGAG CACAGTGGTG AGTCTGCCTC TGGGGCCTGG CAGCCACTGC 
AGCATGGGTG GAGTCTCACA GGTGCCCGCG GAGGCTGAGG AGACGGCATC TGTGGGCAGC 
TGTTTTGCAT CGGAGGACAC AACCGAAGAC TCAGGGGTCA TGAGCTCTCC CTCAGATGTC 
ATCTCCCTGG ACTCACAACA AGATAGCATG AGATCTAAGG ATAAATGGTC CACAGACCAG 
GAGGACTGCA GTGACCAGGA CCTGGCTGGA ACACCAGAAC TGGGTCCCCA AAAGAGCCCA 
TCATGGGGGA AACATGGCTC AGGAAGCTCA ATTTTAAGGA CTGAGAAAGC CGCCATGCCT 
GGGAATGATG ATGAAGATCT GTCCATCACG GGACTCTTCC ATCAGACTCG AGCAGAGCTT 
GACAAAGACC TGGGAGGGAT GGAAGAAAAT TATGAAACAG ATACCAGCTC TCTAACCAGC 
TCTGTAAATG GGGTATCCAA CCACAGTCTG CAAGAAGCCA TAATCCCTGA TAGTGGTGTG 
GACGACATCC CTGTCACTTT TATCGGGGAA GTTTCTGACG AGCCTTTCGA CTCGGGGCTG 
TTCTTCAGTG GAAGTAACAA TGCTGCTGCT TTGAACCAAG GGGGCATTGC CAGCCAAAGA 
TCCCACCTGC CCCCATATCA GACTGAGCAG AGCCAGCCAT TCATAAGGAC AAACAGAAAA 
GAGCCTGACC CCTCCCTTCC TTCCCAAGAT TACAGAAAGA GAAATCAGCC AATCTTAGCC 
AACACATCTG AAAATGAGAA TCCAGTTGGA ATAGACCCCA GAGTTACATC ATTTGTTTCA 
AAGCCTTCGA CAGATGACCC AAAGGCAAAG GATAAAGACA AGATGTGCGG TTCTGGTCCC 
AGTGAGAAGA CACAGACCGG TCACAGAGTA AACTCCCTGC CAGTGAATCC CAGAGTTGGT 
GAGGATGAAA ACTCCAACTC AGCCCTGCCC CCATGGTCTC ACCATGGCCA GGCCTCGGGG 
GGAAGCTATG GACTCAAGTA TGGCCTCACT ACATATAAAA TCGTTCCTCC CAAACCAGAG 
ATGAGGTGTT ATGACAGGGA TGTGTCCCTC TCCACTGGTG CCATTAAGAT TGATGAACTA 
GGGAATCTGA TGAGTCCTCA CATGAATGGA AGCAGGACCA TATCCAAGCC ATCAGCTGTT 
GCCGAAACAG AGGCTCCACC TATTGGAAAA GTCAAAGAGT TCTGGAGGCG CAACTCGATG 
GAAAAGTACC TGAATGGGCC AGCCGAGTGC ACGGTCAAAA AGGCCCCCTC CACCACCATC 
ACTGCCACAT CCGAAAAACC ACAGCGAGAT GAGACGAAGG CAGGCTTTAC GCTGACCACT 
CCGGAGCAGC AGCCTGCCTC ACAGGAATAC GGGGCACCCC CGGAGGAAGA CAGAAGCCGG 
CCACATTCAG CCGTCTCCTG TCCTGTGAAA GTGCCAGCAC CTAATCCCAC AGACATCACA 
TTCCTCAAGC CTCAGAGAAG AACATCCAGC CAGTATGTGG CTTCTGCTAT TGCCAAGAAG 
ATGGGACCTC CAAAAGTCCA TGCTGATGTG GTGAGACCAC ATAAGAAGAC TGCCGAACAA 
GGCCACGAGG AAGCAAAGCT CGCTAGACCA CCTCCAGCCT GGAAAGACAG TGCAGTCCCC 
AACCTGTGTT CAGAGGCCGG CCAGTGTGAG CATGGCACAA ACCAGGGCTC TGTCCGCCTT 
CCCAGCAACC CTGGTGGGCA GTTGCCAGCG GACCATCCCA AAGTAGAGGT CAACAGCACA 
TATGGGAAGT CAGCGACTCA CAACTCTCCT GCTGCTGTCC ACAGAAACTC CTATTTCCTC 
CCAGGCAGAT CTTCCCAGCG GGATCGTGTT TCTGTAGGAC AGAGCTGTGG TTTCCATGAA 
AAGCAAACTA TAAGTAACCA AAAGATGAAT TCAACATCTA ACCCCTCGCA GGCACTGGAC 
AAGGCACACC CAGCCCCCCT GCTCTTGACA GAAGCCCGTG ACTCTGGGAG GATACTGGTG 
AATGGCTCTG CACAGACCCC AGGAAATTGT GAGCCTCCCC ACTCTCAGAA AGAGTCCACC 
CTGACTAGCT ATATTATCTT ACAGACAGAG GAAAAGCCCA GTCCGTTATC TGCAGATGGT 
CAAAACTCAG ATGATGCTCT ACCGTCGAGC ATTTTTGGGC CAAAGAAAAA ATTCAAGCCT 
GTCGTTCAAA GGCCACTTCC AAAAGATATA TCCCTGCACA GTGCCCTGAT GGAAGCCATC 
CACACCTCAG GAGGGAGGGA CAAGCTCCGA AAGACTGCAG AACAGGCCTC AGAAGGAAGG 
CCAAAGAAAC CATCCTACGT GGAGGCGGAG AGCGAGCGAT CTGCCCTTCT GGCAGCCATC 
CGGGGCCACA GTGGGACTCT CAGCCTGAGG AAGGTGTCAT CCCTGGCCTC TGAGGAGCTC 
CAGAGCTTCC GAGATGCTGC ACTCATGGCT CCAGGTGTGG ACAAGCCTCA GCAAGAAGAC 
CGTGGCCTTC CACCCCCACC TGCCCTGCCA CCACCATCCA CTCCAGCATC CCAGGTTCCC 
TCAGCCTCCA TACCGGTTTC TAGGTTCAGC ATCGGCACCC TCAGCAACCC AGTAAATGCT 
AGGCAAGCCT TGATGGATGC TATCCGCTCG GGCACAGGAG CTGCAAGACT CAGAAAGGTT 
CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cobl ( NM_001107236.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100706.1 Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), mRNA.

Price & Availability↑

CloneID	ORa11989
Clone ID Related Accession (Same CDS sequence)	NM_001107236.1
Accession Version	NM_001107236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	protein cordon-bleu
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474055.1. On or before Oct 4, 2007 this sequence version replaced XM_001053778.1, XM_223597.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906348 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGAAA TGCTCAGGAA ATCATCCCTT 
GGCAATGATG AGACAGATAA GGAGAAGAAA AAATTTCTTG GGTTTTTCAA AGTTAATAAG 
AGGAGCAACA GTAAGGCTGA GCATCTTGGG CTGTCGGGCG CAGATAGCGA CGAGGATCCC 
TCGAAGTCAG CGTCTGGAGG GGACTTGAAC GGCTGTGTAA CAACACCCAA CTCACCATCC 
TTGCATTCTC GGTCCCTGAC ACTGGGTCCC TCCCTCTCAC TGGGCAACAT CTCTGGAATG 
TCTATGAAGT CAGACATGAA GAAGCGCAGA GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG 
GGTCAAGACA AGGTATCAGA AAAGGCCTCC CTCAGCTCGC AGGCCGATCT TCAGAAGAAG 
AAGAGGCGGG CGCCAGCTCC TCCTCCACCA CAGCCGCCAC CACCAAGTCC TGTGGTTCCC 
AACCGCAAGG AAGATAAGGA AGAGAACAGG AAGAGCACAG TGGGGATGGA AGAAAATTAT 
GAAACAGATA CCAGCTCTCT AACCAGCTCT GTAAATGGGG TATCCAACCA CAGTCTGCAA 
GAAGCCATAA TCCCTGATAG TGGTGTGGAC GACATCCCTG TCACTTTTAT CGGGGAAGTT 
TCTGACGAGC CTTTCGACTC GGGGCTGTTC TTCAGTGGAA GTAACAATGC TGCTGCTTTG 
AACCAAGGGG GCATTGCCAG CCAAAGATCC CACCTGCCCC CATATCAGAC TGAGCAGAGC 
CAGCCATTCA TAAGGACAAA CAGAAAAGAG CCTGACCCCT CCCTTCCTTC CCAAGATTAC 
AGAAAGAGAA ATCAGCCAAT CTTAGCCAAC ACATCTGAAA ATGAGAATCC AGTTGGAATA 
GACCCCAGAG TTACATCATT TGTTTCAAAG CCTTCGACAG ATGACCCAAA GGCAAAGGAT 
AAAGACAAGA TGTGCGGTTC TGGTCCCAGT GAGAAGACAC AGACCGGTCA CAGAGTAAAC 
TCCCTGCCAG TGAATCCCAG AGTTGGTGAG GATGAAAACT CCAACTCAGC CCTGCCCCCA 
TGGTCTCACC ATGGCCAGGC CTCGGGGGGA AGCTATGGAC TCAAGTATGG CCTCACTACA 
TATAAAATCG TTCCTCCCAA ACCAGAGATG AGGTGTTATG ACAGGGATGT GTCCCTCTCC 
ACTGGTGCCA TTAAGATTGA TGAACTAGGG AATCTGATGA GTCCTCACAT GAATGGAAGC 
AGGACCATAT CCAAGCCATC AGCTGTTGCC GAAACAGAGG CTCCACCTAT TGGAAAAGTC 
AAAGAGTTCT GGAGGCGCAA CTCGATGGAA AAGTACCTGA ATGGGCCAGC CGAGTGCACG 
GTCAAAAAGG CCCCCTCCAC CACCATCACT GCCACATCCG AAAAACCACA GCGAGATGAG 
ACGAAGGCAG GCTTTACGCT GACCACTCCG GAGCAGCAGC CTGCCTCACA GGAATACGGG 
GCACCCCCGG AGGAAGACAG AAGCCGGCCA CATTCAGCCG TCTCCTGTCC TGTGAAAGTG 
CCAGCACCTA ATCCCACAGA CATCACATTC CTCAAGCCTC AGAGAAGAAC ATCCAGCCAG 
TATGTGGCTT CTGCTATTGC CAAGAAGATG GGACCTCCAA AAGTCCATGC TGATGTGGTG 
AGACCACATA AGAAGACTGC CGAACAAGGC CACGAGGAAG CAAAGCTCGC TAGACCACCT 
CCAGCCTGGA AAGACAGTGC AGTCCCCAAC CTGTGTTCAG AGGCCGGCCA GTGTGAGCAT 
GGCACAAACC AGGGCTCTGT CCGCCTTCCC AGCAACCCTG GTGGGCAGTT GCCAGCGGAC 
CATCCCAAAG TAGAGGTCAA CAGCACATAT GGGAAGTCAG CGACTCACAA CTCTCCTGCT 
GCTGTCCACA GAAACTCCTA TTTCCTCCCA GGCAGATCTT CCCAGCGGGA TCGTGTTTCT 
GTAGGACAGA GCTGTGGTTT CCATGAAAAG CAAACTATAA GTAACCAAAA GATGAATTCA 
ACATCTAACC CCTCGCAGGC ACTGGACAAG GCACACCCAG CCCCCCTGCT CTTGACAGAA 
GCCCGTGACT CTGGGAGGAT ACTGGTGAAT GGCTCTGCAC AGACCCCAGG AAATTGTGAG 
CCTCCCCACT CTCAGAAAGA GTCCACCCTG ACTAGCTATA TTATCTTACA GACAGAGGAA 
AAGCCCAGTC CGTTATCTGC AGATGGTCAA AACTCAGATG ATGCTCTACC GTCGAGCATT 
TTTGGGCCAA AGAAAAAATT CAAGCCTGTC GTTCAAAGGC CACTTCCAAA AGATATATCC 
CTGCACAGTG CCCTGATGGA AGCCATCCAC ACCTCAGGAG GGAGGGACAA GCTCCGAAAG 
ACTGCAGAAC AGGCCTCAGA AGGAAGGCCA AAGAAACCAT CCTACGTGGA GGCGGAGAGC 
GAGCGATCTG CCCTTCTGGC AGCCATCCGG GGCCACAGTG GGACTCTCAG CCTGAGGAAG 
GTGTCATCCC TGGCCTCTGA GGAGCTCCAG AGCTTCCGAG ATGCTGCACT CATGGCTCCA 
GGTGTGGACA AGCCTCAGCA AGAAGACCGT GGCCTTCCAC CCCCACCTGC CCTGCCACCA 
CCATCCACTC CAGCATCCCA GGTTCCCTCA GCCTCCATAC CGGTTTCTAG GTTCAGCATC 
GGCACCCTCA GCAACCCAGT AAATGCTAGG CAAGCCTTGA TGGATGCTAT CCGCTCGGGC 
ACAGGAGCTG CAAGACTCAG AAAGGTTCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100706.1
CDS	8..3409
Translation	MKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVHLKEALHNSTLDITVVLPSGLEKQSVVSGSRAVMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNAHTVFLKEKVPEEKGKPGLTKAPEKSVRLVVNYLRTQKAVMRVSPEVPLQNILPVICAKCEVSPDHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRREMLRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEHLGLSGADSDEDPSKSASGGDLNGCVTTPNSPSLHSRSLTLGPSLSLGNISGMSMKSDMKKRRAPPPPSPGLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQPPPPSPVVPNRKEDKEENRKSTVGMEENYETDTSSLTSSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFFSGSNNAAALNQGGIASQRSHLPPYQTEQSQPFIRTNRKEPDPSLPSQDYRKRNQPILANTSENENPVGIDPRVTSFVSKPSTDDPKAKDKDKMCGSGPSEKTQTGHRVNSLPVNPRVGEDENSNSALPPWSHHGQASGGSYGLKYGLTTYKIVPPKPEMRCYDRDVSLSTGAIKIDELGNLMSPHMNGSRTISKPSAVAETEAPPIGKVKEFWRRNSMEKYLNGPAECTVKKAPSTTITATSEKPQRDETKAGFTLTTPEQQPASQEYGAPPEEDRSRPHSAVSCPVKVPAPNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKKTAEQGHEEAKLARPPPAWKDSAVPNLCSEAGQCEHGTNQGSVRLPSNPGGQLPADHPKVEVNSTYGKSATHNSPAAVHRNSYFLPGRSSQRDRVSVGQSCGFHEKQTISNQKMNSTSNPSQALDKAHPAPLLLTEARDSGRILVNGSAQTPGNCEPPHSQKESTLTSYIILQTEEKPSPLSADGQNSDDALPSSIFGPKKKFKPVVQRPLPKDISLHSALMEAIHTSGGRDKLRKTAEQASEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRDAALMAPGVDKPQQEDRGLPPPPALPPPSTPASQVPSASIPVSRFSIGTLSNPVNARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_001107236.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cordon-bleu WH2 repeat protein (Cobl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107236.1

ORF Insert Sequence:

ATGAAGGCCC GCGCTCCACC ACCTCCTGGA AAGCCTGCTG CCCAGAATGT CCACAGCGAA 
CAGAAACTGC CTCATGACGC CACACTGGGT TCACAGCAAA GCCTGGTCCA CTTGAAGGAG 
GCACTTCACA ACAGTACCTT GGACATCACT GTGGTTCTGC CCAGTGGACT GGAGAAGCAG 
AGCGTGGTTA GTGGGAGCCG TGCGGTGATG GACCTCCTGG TTGAACTCTG CCTTCAGAAC 
CACCTGAATC CTTCTCACCA CGTCTTAGAG ATCTGGTCTT CGGAGACCCA ACAGCCTTTG 
AGTTTTAAGC CAAATACGTT GATTGGCTCC TTGAATGCAC ATACGGTATT TCTGAAAGAA 
AAAGTCCCTG AAGAGAAAGG GAAGCCTGGC CTAACCAAGG CCCCTGAGAA ATCCGTACGC 
CTGGTAGTGA ACTACCTGAG AACTCAGAAG GCTGTCATGC GGGTGAGCCC TGAGGTGCCT 
CTGCAGAACA TTCTGCCTGT CATCTGTGCG AAGTGTGAGG TCAGCCCAGA TCACGTGGTG 
CTGCTCAGGG ACAATATTGC TGGGGAAGAG CTGGAGCTGT CCAAGTCCCT GAATGAACTA 
GGGATCAAGG AGCTATATGC GTGGGACAAC AGAAGAGAAA TGCTCAGGAA ATCATCCCTT 
GGCAATGATG AGACAGATAA GGAGAAGAAA AAATTTCTTG GGTTTTTCAA AGTTAATAAG 
AGGAGCAACA GTAAGGCTGA GCATCTTGGG CTGTCGGGCG CAGATAGCGA CGAGGATCCC 
TCGAAGTCAG CGTCTGGAGG GGACTTGAAC GGCTGTGTAA CAACACCCAA CTCACCATCC 
TTGCATTCTC GGTCCCTGAC ACTGGGTCCC TCCCTCTCAC TGGGCAACAT CTCTGGAATG 
TCTATGAAGT CAGACATGAA GAAGCGCAGA GCCCCTCCTC CTCCAAGTCC CGGGCTGCTG 
GGTCAAGACA AGGTATCAGA AAAGGCCTCC CTCAGCTCGC AGGCCGATCT TCAGAAGAAG 
AAGAGGCGGG CGCCAGCTCC TCCTCCACCA CAGCCGCCAC CACCAAGTCC TGTGGTTCCC 
AACCGCAAGG AAGATAAGGA AGAGAACAGG AAGAGCACAG TGGGGATGGA AGAAAATTAT 
GAAACAGATA CCAGCTCTCT AACCAGCTCT GTAAATGGGG TATCCAACCA CAGTCTGCAA 
GAAGCCATAA TCCCTGATAG TGGTGTGGAC GACATCCCTG TCACTTTTAT CGGGGAAGTT 
TCTGACGAGC CTTTCGACTC GGGGCTGTTC TTCAGTGGAA GTAACAATGC TGCTGCTTTG 
AACCAAGGGG GCATTGCCAG CCAAAGATCC CACCTGCCCC CATATCAGAC TGAGCAGAGC 
CAGCCATTCA TAAGGACAAA CAGAAAAGAG CCTGACCCCT CCCTTCCTTC CCAAGATTAC 
AGAAAGAGAA ATCAGCCAAT CTTAGCCAAC ACATCTGAAA ATGAGAATCC AGTTGGAATA 
GACCCCAGAG TTACATCATT TGTTTCAAAG CCTTCGACAG ATGACCCAAA GGCAAAGGAT 
AAAGACAAGA TGTGCGGTTC TGGTCCCAGT GAGAAGACAC AGACCGGTCA CAGAGTAAAC 
TCCCTGCCAG TGAATCCCAG AGTTGGTGAG GATGAAAACT CCAACTCAGC CCTGCCCCCA 
TGGTCTCACC ATGGCCAGGC CTCGGGGGGA AGCTATGGAC TCAAGTATGG CCTCACTACA 
TATAAAATCG TTCCTCCCAA ACCAGAGATG AGGTGTTATG ACAGGGATGT GTCCCTCTCC 
ACTGGTGCCA TTAAGATTGA TGAACTAGGG AATCTGATGA GTCCTCACAT GAATGGAAGC 
AGGACCATAT CCAAGCCATC AGCTGTTGCC GAAACAGAGG CTCCACCTAT TGGAAAAGTC 
AAAGAGTTCT GGAGGCGCAA CTCGATGGAA AAGTACCTGA ATGGGCCAGC CGAGTGCACG 
GTCAAAAAGG CCCCCTCCAC CACCATCACT GCCACATCCG AAAAACCACA GCGAGATGAG 
ACGAAGGCAG GCTTTACGCT GACCACTCCG GAGCAGCAGC CTGCCTCACA GGAATACGGG 
GCACCCCCGG AGGAAGACAG AAGCCGGCCA CATTCAGCCG TCTCCTGTCC TGTGAAAGTG 
CCAGCACCTA ATCCCACAGA CATCACATTC CTCAAGCCTC AGAGAAGAAC ATCCAGCCAG 
TATGTGGCTT CTGCTATTGC CAAGAAGATG GGACCTCCAA AAGTCCATGC TGATGTGGTG 
AGACCACATA AGAAGACTGC CGAACAAGGC CACGAGGAAG CAAAGCTCGC TAGACCACCT 
CCAGCCTGGA AAGACAGTGC AGTCCCCAAC CTGTGTTCAG AGGCCGGCCA GTGTGAGCAT 
GGCACAAACC AGGGCTCTGT CCGCCTTCCC AGCAACCCTG GTGGGCAGTT GCCAGCGGAC 
CATCCCAAAG TAGAGGTCAA CAGCACATAT GGGAAGTCAG CGACTCACAA CTCTCCTGCT 
GCTGTCCACA GAAACTCCTA TTTCCTCCCA GGCAGATCTT CCCAGCGGGA TCGTGTTTCT 
GTAGGACAGA GCTGTGGTTT CCATGAAAAG CAAACTATAA GTAACCAAAA GATGAATTCA 
ACATCTAACC CCTCGCAGGC ACTGGACAAG GCACACCCAG CCCCCCTGCT CTTGACAGAA 
GCCCGTGACT CTGGGAGGAT ACTGGTGAAT GGCTCTGCAC AGACCCCAGG AAATTGTGAG 
CCTCCCCACT CTCAGAAAGA GTCCACCCTG ACTAGCTATA TTATCTTACA GACAGAGGAA 
AAGCCCAGTC CGTTATCTGC AGATGGTCAA AACTCAGATG ATGCTCTACC GTCGAGCATT 
TTTGGGCCAA AGAAAAAATT CAAGCCTGTC GTTCAAAGGC CACTTCCAAA AGATATATCC 
CTGCACAGTG CCCTGATGGA AGCCATCCAC ACCTCAGGAG GGAGGGACAA GCTCCGAAAG 
ACTGCAGAAC AGGCCTCAGA AGGAAGGCCA AAGAAACCAT CCTACGTGGA GGCGGAGAGC 
GAGCGATCTG CCCTTCTGGC AGCCATCCGG GGCCACAGTG GGACTCTCAG CCTGAGGAAG 
GTGTCATCCC TGGCCTCTGA GGAGCTCCAG AGCTTCCGAG ATGCTGCACT CATGGCTCCA 
GGTGTGGACA AGCCTCAGCA AGAAGACCGT GGCCTTCCAC CCCCACCTGC CCTGCCACCA 
CCATCCACTC CAGCATCCCA GGTTCCCTCA GCCTCCATAC CGGTTTCTAG GTTCAGCATC 
GGCACCCTCA GCAACCCAGT AAATGCTAGG CAAGCCTTGA TGGATGCTAT CCGCTCGGGC 
ACAGGAGCTG CAAGACTCAG AAAGGTTCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.
PLoS biology13(9)e1002233(2015)
Hou W,Izadi M,Nemitz S,Haag N,Kessels MM,Qualmann B

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Molecular biology of the cell23(2)324-36(2012 Jan)
Revenu C,Ubelmann F,Hurbain I,El-Marjou F,Dingli F,Loew D,Delacour D,Gilet J,Brot-Laroche E,Rivero F,Louvard D,Robine S

The functions of the actin nucleator Cobl in cellular morphogenesis critically depend on syndapin I.
The EMBO journal30(15)3147-59(2011 Jul)
Schwintzer L,Koch N,Ahuja R,Grimm J,Kessels MM,Qualmann B

Cordon-bleu is an actin nucleation factor and controls neuronal morphology.
Cell131(2)337-50(2007 Oct)
Ahuja R,Pinyol R,Reichenbach N,Custer L,Klingensmith J,Kessels MM,Qualmann B

Cordon-bleu is a conserved gene involved in neural tube formation.
Developmental biology262(1)16-31(2003 Oct)
Carroll EA,Gerrelli D,Gasca S,Berg E,Beier DR,Copp AJ,Klingensmith J

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Cobl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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