Adamts3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adamts3
Entrez Gene ID	305253
Full Name	ADAM metallopeptidase with thrombospondin type 1, motif 3
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1, motif 3 Names A disintegrin and metalloproteinase with thrombospondin motifs 3 a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250774.3	XP_006250836.1	A disintegrin and metalloproteinase with thrombospondin motifs 3 isoform X2
XM_006250773.3	XP_006250835.1	A disintegrin and metalloproteinase with thrombospondin motifs 3 isoform X1
NM_001107212.1	NP_001100682.1	A disintegrin and metalloproteinase with thrombospondin motifs 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ADAMTS3	XP_517240.2
	ADAMTS3	XP_001104686.2
Canis lupus familiaris (dog)	ADAMTS3	XP_539311.3
Homo sapiens (human)	ADAMTS3	NP_055058.2
Danio rerio (zebrafish)	LOC568788	XP_005165504.1
Mus musculus (house mouse)	Adamts3	NP_808540.2
Rattus norvegicus (Norway rat)	Adamts3	NP_001100682.1
Xenopus tropicalis (tropical clawed frog)	adamts3	XP_002940398.2
Bos taurus (cattle)	ADAMTS3	NP_001179726.1
Gallus gallus (chicken)	ADAMTS3	XP_426316.4

Gene Ontology
Bibliography

Related articles in PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wilson R, Geyer SH, Reissig L, Rose J, Szumska D, Hardman E, Prin F, McGuire C, Ramirez-Solis R, White J, Galli A, Tudor C, Tuck E, Mazzeo CI, Smith JC, Robertson E, Adams DJ, Mohun T, Weninger WJ
Wellcome open research11(2016)

CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation.
Jeltsch M, Jha SK, Tvorogov D, Anisimov A, Lepp?nen VM, Holopainen T, Kivel? R, Ortega S, K?rpanen T, Alitalo K
Circulation129(19)1962-71(2014 May)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Regulation of procollagen amino-propeptide processing during mouse embryogenesis by specialization of homologous ADAMTS proteases: insights on collagen biosynthesis and dermatosparaxis.
Le Goff C, Somerville RP, Kesteloot F, Powell K, Birk DE, Colige AC, Apte SS
Development (Cambridge, England)133(8)1587-96(2006 Apr)

Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis.
Fernandes RJ, Hirohata S, Engle JM, Colige A, Cohn DH, Eyre DR, Apte SS
The Journal of biological chemistry276(34)31502-9(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adamts3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adamts3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11988	NM_001107212.1 Latest version!	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa35926	XM_006250773.3 Latest version!	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa35927	XM_006250774.3 Latest version!	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adamts3 ( NM_001107212.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100682.1 Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), mRNA.

Price & Availability↑

CloneID	ORa11988
Clone ID Related Accession (Same CDS sequence)	NM_001107212.1
Accession Version	NM_001107212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474060.2. On or before Oct 4, 2007 this sequence version replaced XM_223279.4, XM_001072843.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GTACCTTAAG ATGTTTGATA TTCCCCCTGG GGCTAGACAT 
GTCTTAATCC AAGAAGACGA GGCTTCTCCT CATGTTCTTG CCATTAAGAA CCAAGCCACA 
GGTCACTACA TTTTAAATGG CAAAGGGGAG GAAGCCAAGT CACGGACCTT CATAGACCTT 
GGTGTGGAGT GGGATTACAA CATTGAAGAT GACATTGAAA CTCTCCACAC GGATGGGCCA 
CTACACGACC CCGTTATTGT TCTGATCATA CCTCAGGAGA ACGACACGCG CTCTAGCCTG 
ACGTATAAGT ACATCATCCA TGAAGACTCC GTGCCAACAA TCAACAGTAA CAACGTCATC 
CAGGAGGAGC TGGACACCTT CGAGTGGGCG CTGAAGAGCT GGTCTCAGTG CTCCAAACCC 
TGTGGTGGAG GGTTCCAGTA TACGAAATAC GGATGCCGAA GGAAAAGCGA CAGTAAAATG 
GTTCACCGCA GCTTCTGTGA GGTCAATAAG AAGCCGAAAC CCATCAGACG CATGTGCAAC 
ATTCAGGAGT GCACACACCC GCTTGTTCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100682.1
CDS	451..3183
Translation	MVFLSLWLIAASLVEVRTSADGQAGAEEMVQIDLPTKRYREYELVTPVSTNLKGHYLSHILSANHKKRSPRDVSSNSEQLFFNVTAFGRDFHLRLRPNTHFIAPGAVVEWHETAPRPGNTTDPRNSRPHRSSSEGGWRSEPLQTSCAYVGDIMDVPGTSVAISNCDGLAGMIKSDNEEYFIEPLERGKQMDEENGRIHIVYKRSAMERAPRDVSEDVYYKESNLEGLDDLGTVYSNIGQQLNETTRRRRHAGENDYNIEVLLGVDDSVVRFHGKEHVQNYLLTLMNIVNEIYHDESLGVHINVVLVRMIMLGYAKSISLIERGNPSRSLENVCRWACQQQKSDPNHAEHHDHAIFLTRQDFGPAGMQGYAPVTGMCHPVRSCTLNHEDGFSSAFVVAHETGHVLGMEHDGQGNRCGDETAMGSVMAPLVQAAFHRYHWSRCSGQELKRYIHSYDCLLDDPFEHDWPKLPELPGINYSMDEQCRFDFGVGYKMCTAFRTFDPCKQLWCSHPDNPYFCKTKKGPPLDGTECAAGKWCYKGHCMWKNANQQKQDGNWGSWTKFGSCSRTCGTGVRFRTRQCNNPTPINGGQDCPGVNFEYQLCNTEECQKHFEDFRAQQCQQRNSHFDYQNTKHHWLPHEHPDSKKRCHLYCQSKETGDVAYMKQLVHDGTRCSYKDPYSICVRGECVKVGCDKEIGSNKVEDKCGVCGGDNSHCRTVKGTFTRTPRKLGYLKMFDIPPGARHVLIQEDEASPHVLAIKNQATGHYILNGKGEEAKSRTFIDLGVEWDYNIEDDIETLHTDGPLHDPVIVLIIPQENDTRSSLTYKYIIHEDSVPTINSNNVIQEELDTFEWALKSWSQCSKPCGGGFQYTKYGCRRKSDSKMVHRSFCEVNKKPKPIRRMCNIQECTHPLVR

Target ORF information:

RefSeq Version	NM_001107212.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107212.1

ORF Insert Sequence:

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GTACCTTAAG ATGTTTGATA TTCCCCCTGG GGCTAGACAT 
GTCTTAATCC AAGAAGACGA GGCTTCTCCT CATGTTCTTG CCATTAAGAA CCAAGCCACA 
GGTCACTACA TTTTAAATGG CAAAGGGGAG GAAGCCAAGT CACGGACCTT CATAGACCTT 
GGTGTGGAGT GGGATTACAA CATTGAAGAT GACATTGAAA CTCTCCACAC GGATGGGCCA 
CTACACGACC CCGTTATTGT TCTGATCATA CCTCAGGAGA ACGACACGCG CTCTAGCCTG 
ACGTATAAGT ACATCATCCA TGAAGACTCC GTGCCAACAA TCAACAGTAA CAACGTCATC 
CAGGAGGAGC TGGACACCTT CGAGTGGGCG CTGAAGAGCT GGTCTCAGTG CTCCAAACCC 
TGTGGTGGAG GGTTCCAGTA TACGAAATAC GGATGCCGAA GGAAAAGCGA CAGTAAAATG 
GTTCACCGCA GCTTCTGTGA GGTCAATAAG AAGCCGAAAC CCATCAGACG CATGTGCAAC 
ATTCAGGAGT GCACACACCC GCTTGTTCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adamts3 ( XM_006250773.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250835.1 Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35926
Clone ID Related Accession (Same CDS sequence)	XM_006250773.3
Accession Version	XM_006250773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GTACCTTAAG ATGTTTGATA TTCCCCCTGG GGCTAGACAT 
GTCTTAATCC AAGAAGACGA GGCTTCTCCT CATGTTCTTG CCATTAAGAA CCAAGCCACA 
GGTCACTACA TTTTAAATGG CAAAGGGGAG GAAGCCAAGT CACGGACCTT CATAGACCTT 
GGTGTGGAGT GGGATTACAA CATTGAAGAT GACATTGAAA CTCTCCACAC GGATGGGCCA 
CTACACGACC CCGTTATTGT TCTGATCATA CCTCAGGAGA ACGACACGCG CTCTAGCCTG 
ACGTATAAGT ACATCATCCA TGAAGACTCC GTGCCAACAA TCAACAGTAA CAACGTCATC 
CAGGAGGAGC TGGACACCTT CGAGTGGGCG CTGAAGAGCT GGTCTCAGTG CTCCAAACCC 
TGTGGTGGAG GGTTCCAGTA TACGAAATAC GGATGCCGAA GGAAAAGCGA CAGTAAAATG 
GTTCACCGCA GCTTCTGTGA GGTCAATAAG AAGCCGAAAC CCATCAGACG CATGTGCAAC 
ATTCAGGAGT GCACACACCC GCTCTGGGCA GCAGAAGCGT GGGAACACTG CAGCAGAAGC 
TGTGGCAGCT CGGGGTACCA GCTACGCACC GTACGCTGCC TCCAACCACT CCAGGACGGT 
ACCAACCGCT CAGTGCACAG CAGGTACTGC CTGGGAGAGC GCCCGGAGAG CCGCCGGCCC 
TGTAATAGAA TACCCTGCCC AGCCCAGTGG AAAACAGGGC CCTGGAATGA GTGTTCGGTG 
ACCTGCGGTG AGGGAACAGA GGTGAGACAG GTTCTCTGTA GGACTGGAGA CCACTGTGAT 
GGGGAAAAGC CCGAGTCTGT CAGAGCCTGC CAGCTGCCTC CCTGTAATGA CGAGCCGTGC 
TTGGGCGACA AGTCCATATT CTGTCAAATG GAGGTGCTAG CAAGATACTG CTCCATACCA 
GGCTACAGCG AGCTGTGCTG CGAGTCATGT AGCAAGCGCA GTGGCACCCT GCCACCGCCA 
TACCTTCAAG AAGCTGCTGA AATCCACAGT GATGCCATCT TTGGCCCTAG TGACCTCCCT 
AGGTCCCTAG TGATGCCCAC ACCCTTCCTC CCTTACTACT CAGGGTCCCG TGCCGACAAG 
AAATCCTTGA GTAGCGTCTC CTCCGTGGCA AATCCCAGCG CATATGCTGC TTTCAGATCC 
AGCAGCAGAG CCAGTGTTAG CTTCCCCCGG AAGAAAGCAC AAGAGGGTGG AACAAAGACG 
CCGGTATCAA TGCCACCCTC CTCATCCATA AAGACTGATC ACCTCCATTC CTCTTCCCCT 
GGGGCTGCTG CCCCCTCCCC TGCTATCACT GATTCCATAG GTGCTTCTCC CCAGACAAGA 
ACCCCAAAGA AAGATGGAAA GACTATTAGC AGGAGACATC CGATCAGGTC AACCGCCTCA 
GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250835.1
CDS	580..4188
Translation	MVFLSLWLIAASLVEVRTSADGQAGAEEMVQIDLPTKRYREYELVTPVSTNLKGHYLSHILSANHKKRSPRDVSSNSEQLFFNVTAFGRDFHLRLRPNTHFIAPGAVVEWHETAPRPGNTTDPRNSRPHRSSSEGGWRSEPLQTSCAYVGDIMDVPGTSVAISNCDGLAGMIKSDNEEYFIEPLERGKQMDEENGRIHIVYKRSAMERAPRDVSEDVYYKESNLEGLDDLGTVYSNIGQQLNETTRRRRHAGENDYNIEVLLGVDDSVVRFHGKEHVQNYLLTLMNIVNEIYHDESLGVHINVVLVRMIMLGYAKSISLIERGNPSRSLENVCRWACQQQKSDPNHAEHHDHAIFLTRQDFGPAGMQGYAPVTGMCHPVRSCTLNHEDGFSSAFVVAHETGHVLGMEHDGQGNRCGDETAMGSVMAPLVQAAFHRYHWSRCSGQELKRYIHSYDCLLDDPFEHDWPKLPELPGINYSMDEQCRFDFGVGYKMCTAFRTFDPCKQLWCSHPDNPYFCKTKKGPPLDGTECAAGKWCYKGHCMWKNANQQKQDGNWGSWTKFGSCSRTCGTGVRFRTRQCNNPTPINGGQDCPGVNFEYQLCNTEECQKHFEDFRAQQCQQRNSHFDYQNTKHHWLPHEHPDSKKRCHLYCQSKETGDVAYMKQLVHDGTRCSYKDPYSICVRGECVKVGCDKEIGSNKVEDKCGVCGGDNSHCRTVKGTFTRTPRKLGYLKMFDIPPGARHVLIQEDEASPHVLAIKNQATGHYILNGKGEEAKSRTFIDLGVEWDYNIEDDIETLHTDGPLHDPVIVLIIPQENDTRSSLTYKYIIHEDSVPTINSNNVIQEELDTFEWALKSWSQCSKPCGGGFQYTKYGCRRKSDSKMVHRSFCEVNKKPKPIRRMCNIQECTHPLWAAEAWEHCSRSCGSSGYQLRTVRCLQPLQDGTNRSVHSRYCLGERPESRRPCNRIPCPAQWKTGPWNECSVTCGEGTEVRQVLCRTGDHCDGEKPESVRACQLPPCNDEPCLGDKSIFCQMEVLARYCSIPGYSELCCESCSKRSGTLPPPYLQEAAEIHSDAIFGPSDLPRSLVMPTPFLPYYSGSRADKKSLSSVSSVANPSAYAAFRSSSRASVSFPRKKAQEGGTKTPVSMPPSSSIKTDHLHSSSPGAAAPSPAITDSIGASPQTRTPKKDGKTISRRHPIRSTASER

Target ORF information:

RefSeq Version	XM_006250773.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250773.3

ORF Insert Sequence:

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GTACCTTAAG ATGTTTGATA TTCCCCCTGG GGCTAGACAT 
GTCTTAATCC AAGAAGACGA GGCTTCTCCT CATGTTCTTG CCATTAAGAA CCAAGCCACA 
GGTCACTACA TTTTAAATGG CAAAGGGGAG GAAGCCAAGT CACGGACCTT CATAGACCTT 
GGTGTGGAGT GGGATTACAA CATTGAAGAT GACATTGAAA CTCTCCACAC GGATGGGCCA 
CTACACGACC CCGTTATTGT TCTGATCATA CCTCAGGAGA ACGACACGCG CTCTAGCCTG 
ACGTATAAGT ACATCATCCA TGAAGACTCC GTGCCAACAA TCAACAGTAA CAACGTCATC 
CAGGAGGAGC TGGACACCTT CGAGTGGGCG CTGAAGAGCT GGTCTCAGTG CTCCAAACCC 
TGTGGTGGAG GGTTCCAGTA TACGAAATAC GGATGCCGAA GGAAAAGCGA CAGTAAAATG 
GTTCACCGCA GCTTCTGTGA GGTCAATAAG AAGCCGAAAC CCATCAGACG CATGTGCAAC 
ATTCAGGAGT GCACACACCC GCTCTGGGCA GCAGAAGCGT GGGAACACTG CAGCAGAAGC 
TGTGGCAGCT CGGGGTACCA GCTACGCACC GTACGCTGCC TCCAACCACT CCAGGACGGT 
ACCAACCGCT CAGTGCACAG CAGGTACTGC CTGGGAGAGC GCCCGGAGAG CCGCCGGCCC 
TGTAATAGAA TACCCTGCCC AGCCCAGTGG AAAACAGGGC CCTGGAATGA GTGTTCGGTG 
ACCTGCGGTG AGGGAACAGA GGTGAGACAG GTTCTCTGTA GGACTGGAGA CCACTGTGAT 
GGGGAAAAGC CCGAGTCTGT CAGAGCCTGC CAGCTGCCTC CCTGTAATGA CGAGCCGTGC 
TTGGGCGACA AGTCCATATT CTGTCAAATG GAGGTGCTAG CAAGATACTG CTCCATACCA 
GGCTACAGCG AGCTGTGCTG CGAGTCATGT AGCAAGCGCA GTGGCACCCT GCCACCGCCA 
TACCTTCAAG AAGCTGCTGA AATCCACAGT GATGCCATCT TTGGCCCTAG TGACCTCCCT 
AGGTCCCTAG TGATGCCCAC ACCCTTCCTC CCTTACTACT CAGGGTCCCG TGCCGACAAG 
AAATCCTTGA GTAGCGTCTC CTCCGTGGCA AATCCCAGCG CATATGCTGC TTTCAGATCC 
AGCAGCAGAG CCAGTGTTAG CTTCCCCCGG AAGAAAGCAC AAGAGGGTGG AACAAAGACG 
CCGGTATCAA TGCCACCCTC CTCATCCATA AAGACTGATC ACCTCCATTC CTCTTCCCCT 
GGGGCTGCTG CCCCCTCCCC TGCTATCACT GATTCCATAG GTGCTTCTCC CCAGACAAGA 
ACCCCAAAGA AAGATGGAAA GACTATTAGC AGGAGACATC CGATCAGGTC AACCGCCTCA 
GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adamts3 ( XM_006250774.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250836.1 Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X2, mRNA.

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CloneID	ORa35927
Clone ID Related Accession (Same CDS sequence)	XM_006250774.3
Accession Version	XM_006250774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GAAGACGAGA GGCGCATTCG TTTTGCCCAA AGGAGCTCGT 
AATATCTCTC TTGCGGAAAC AAGAGAAACT AAAAATGTAC TGGCCATTAA GAACCAAGCC 
ACAGGTCACT ACATTTTAAA TGGCAAAGGG GAGGAAGCCA AGTCACGGAC CTTCATAGAC 
CTTGGTGTGG AGTGGGATTA CAACATTGAA GATGACATTG AAACTCTCCA CACGGATGGG 
CCACTACACG ACCCCGTTAT TGTTCTGATC ATACCTCAGG AGAACGACAC GCGCTCTAGC 
CTGACGTATA AGTACATCAT CCATGAAGAC TCCGTGCCAA CAATCAACAG TAACAACGTC 
ATCCAGGAGG AGCTGGACAC CTTCGAGTGG GCGCTGAAGA GCTGGTCTCA GTGCTCCAAA 
CCCTGTGGTG GAGGGTTCCA GTATACGAAA TACGGATGCC GAAGGAAAAG CGACAGTAAA 
ATGGTTCACC GCAGCTTCTG TGAGGTCAAT AAGAAGCCGA AACCCATCAG ACGCATGTGC 
AACATTCAGG AGTGCACACA CCCGCTCTGG GCAGCAGAAG CGTGGGAACA CTGCAGCAGA 
AGCTGTGGCA GCTCGGGGTA CCAGCTACGC ACCGTACGCT GCCTCCAACC ACTCCAGGAC 
GGTACCAACC GCTCAGTGCA CAGCAGGTAC TGCCTGGGAG AGCGCCCGGA GAGCCGCCGG 
CCCTGTAATA GAATACCCTG CCCAGCCCAG TGGAAAACAG GGCCCTGGAA TGAGTGTTCG 
GTGACCTGCG GTGAGGGAAC AGAGGTGAGA CAGGTTCTCT GTAGGACTGG AGACCACTGT 
GATGGGGAAA AGCCCGAGTC TGTCAGAGCC TGCCAGCTGC CTCCCTGTAA TGACGAGCCG 
TGCTTGGGCG ACAAGTCCAT ATTCTGTCAA ATGGAGGTGC TAGCAAGATA CTGCTCCATA 
CCAGGCTACA GCGAGCTGTG CTGCGAGTCA TGTAGCAAGC GCAGTGGCAC CCTGCCACCG 
CCATACCTTC AAGAAGCTGC TGAAATCCAC AGTGATGCCA TCTTTGGCCC TAGTGACCTC 
CCTAGGTCCC TAGTGATGCC CACACCCTTC CTCCCTTACT ACTCAGGGTC CCGTGCCGAC 
AAGAAATCCT TGAGTAGCGT CTCCTCCGTG GCAAATCCCA GCGCATATGC TGCTTTCAGA 
TCCAGCAGCA GAGCCAGTGT TAGCTTCCCC CGGAAGAAAG CACAAGAGGG TGGAACAAAG 
ACGCCGGTAT CAATGCCACC CTCCTCATCC ATAAAGACTG ATCACCTCCA TTCCTCTTCC 
CCTGGGGCTG CTGCCCCCTC CCCTGCTATC ACTGATTCCA TAGGTGCTTC TCCCCAGACA 
AGAACCCCAA AGAAAGATGG AAAGACTATT AGCAGGAGAC ATCCGATCAG GTCAACCGCC 
TCAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250836.1
CDS	581..4192
Translation	MVFLSLWLIAASLVEVRTSADGQAGAEEMVQIDLPTKRYREYELVTPVSTNLKGHYLSHILSANHKKRSPRDVSSNSEQLFFNVTAFGRDFHLRLRPNTHFIAPGAVVEWHETAPRPGNTTDPRNSRPHRSSSEGGWRSEPLQTSCAYVGDIMDVPGTSVAISNCDGLAGMIKSDNEEYFIEPLERGKQMDEENGRIHIVYKRSAMERAPRDVSEDVYYKESNLEGLDDLGTVYSNIGQQLNETTRRRRHAGENDYNIEVLLGVDDSVVRFHGKEHVQNYLLTLMNIVNEIYHDESLGVHINVVLVRMIMLGYAKSISLIERGNPSRSLENVCRWACQQQKSDPNHAEHHDHAIFLTRQDFGPAGMQGYAPVTGMCHPVRSCTLNHEDGFSSAFVVAHETGHVLGMEHDGQGNRCGDETAMGSVMAPLVQAAFHRYHWSRCSGQELKRYIHSYDCLLDDPFEHDWPKLPELPGINYSMDEQCRFDFGVGYKMCTAFRTFDPCKQLWCSHPDNPYFCKTKKGPPLDGTECAAGKWCYKGHCMWKNANQQKQDGNWGSWTKFGSCSRTCGTGVRFRTRQCNNPTPINGGQDCPGVNFEYQLCNTEECQKHFEDFRAQQCQQRNSHFDYQNTKHHWLPHEHPDSKKRCHLYCQSKETGDVAYMKQLVHDGTRCSYKDPYSICVRGECVKVGCDKEIGSNKVEDKCGVCGGDNSHCRTVKGTFTRTPRKLGKTRGAFVLPKGARNISLAETRETKNVLAIKNQATGHYILNGKGEEAKSRTFIDLGVEWDYNIEDDIETLHTDGPLHDPVIVLIIPQENDTRSSLTYKYIIHEDSVPTINSNNVIQEELDTFEWALKSWSQCSKPCGGGFQYTKYGCRRKSDSKMVHRSFCEVNKKPKPIRRMCNIQECTHPLWAAEAWEHCSRSCGSSGYQLRTVRCLQPLQDGTNRSVHSRYCLGERPESRRPCNRIPCPAQWKTGPWNECSVTCGEGTEVRQVLCRTGDHCDGEKPESVRACQLPPCNDEPCLGDKSIFCQMEVLARYCSIPGYSELCCESCSKRSGTLPPPYLQEAAEIHSDAIFGPSDLPRSLVMPTPFLPYYSGSRADKKSLSSVSSVANPSAYAAFRSSSRASVSFPRKKAQEGGTKTPVSMPPSSSIKTDHLHSSSPGAAAPSPAITDSIGASPQTRTPKKDGKTISRRHPIRSTASER

Target ORF information:

RefSeq Version	XM_006250774.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1, motif 3 (Adamts3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250774.3

ORF Insert Sequence:

ATGGTGTTCC TGTCACTTTG GTTGATAGCA GCCTCTCTGG TAGAGGTTAG GACATCAGCT 
GATGGACAAG CTGGTGCTGA AGAAATGGTA CAAATAGATT TACCAACCAA GAGATACAGA 
GAGTATGAGC TGGTGACACC AGTTAGCACA AATCTAAAAG GACACTATCT TTCCCATATT 
CTTTCTGCAA ATCACAAAAA GAGGTCACCT AGGGACGTGT CATCCAACTC AGAGCAATTG 
TTCTTCAACG TCACGGCATT TGGGAGAGAT TTTCATCTTC GACTAAGGCC AAACACTCAC 
TTCATAGCTC CTGGGGCTGT GGTGGAATGG CATGAGACAG CTCCAAGGCC TGGGAACACC 
ACTGACCCAA GAAACAGCCG TCCACACAGA AGTTCTTCAG AAGGAGGCTG GAGATCAGAG 
CCTTTGCAGA CTAGCTGTGC GTATGTTGGT GACATCATGG ATGTTCCAGG AACCTCTGTT 
GCCATTAGCA ACTGTGATGG TCTGGCTGGA ATGATAAAGA GTGATAATGA AGAGTACTTC 
ATTGAACCCT TGGAGAGAGG GAAGCAGATG GATGAAGAAA ATGGAAGGAT TCACATTGTC 
TACAAGAGAT CAGCAATGGA GCGGGCTCCC AGAGATGTGT CTGAAGACGT CTACTACAAG 
GAGTCGAATC TGGAAGGCCT CGATGATCTA GGTACCGTTT ACAGCAACAT TGGCCAGCAG 
CTGAATGAGA CAACGCGGCG CCGCAGACAC GCGGGAGAGA ACGATTACAA CATCGAGGTG 
CTTCTGGGAG TGGATGACTC TGTGGTCCGT TTCCATGGCA AAGAGCATGT CCAAAACTAC 
CTCCTCACCC TGATGAACAT TGTAAATGAA ATCTATCACG ATGAGTCCCT TGGAGTGCAC 
ATAAACGTGG TCCTGGTGCG AATGATTATG CTCGGTTACG CAAAGTCTAT CAGCCTTATC 
GAAAGGGGAA ACCCGTCCAG GAGCCTGGAG AATGTGTGTC GCTGGGCTTG CCAGCAACAA 
AAATCCGATC CCAACCATGC TGAACACCAT GATCATGCAA TTTTCCTAAC CAGGCAAGAC 
TTTGGGCCTG CTGGAATGCA AGGATATGCT CCCGTCACAG GCATGTGTCA CCCAGTGAGA 
AGTTGCACCC TGAATCACGA AGATGGTTTT TCATCTGCTT TTGTAGTAGC CCATGAGACC 
GGCCATGTGT TGGGCATGGA GCATGACGGG CAAGGCAACC GGTGTGGCGA TGAGACCGCC 
ATGGGCAGTG TCATGGCGCC CCTGGTGCAA GCTGCCTTTC ATCGATACCA CTGGTCCAGA 
TGCAGCGGTC AGGAACTGAA AAGATATATC CATTCCTACG ACTGTCTCCT TGATGACCCC 
TTTGAACACG ATTGGCCCAA ACTCCCAGAA CTTCCGGGGA TTAATTATTC CATGGATGAG 
CAATGCCGTT TCGATTTTGG TGTTGGCTAC AAAATGTGTA CCGCGTTCCG AACCTTTGAC 
CCGTGCAAGC AGCTGTGGTG CAGTCACCCC GATAACCCAT ACTTCTGTAA GACTAAGAAG 
GGGCCCCCAC TCGATGGGAC TGAGTGTGCT GCTGGGAAGT GGTGCTATAA AGGACACTGC 
ATGTGGAAAA ACGCTAACCA ACAAAAGCAA GATGGCAACT GGGGGTCTTG GACCAAATTT 
GGCTCCTGTT CTAGGACGTG TGGAACTGGT GTTCGTTTTA GAACACGCCA GTGCAATAAT 
CCCACGCCCA TCAATGGTGG GCAGGACTGC CCTGGTGTTA ATTTTGAGTA CCAGCTTTGT 
AATACAGAAG AATGCCAAAA GCACTTTGAG GACTTCAGGG CCCAGCAGTG CCAACAGCGT 
AACTCACACT TTGACTACCA GAATACCAAA CACCACTGGC TGCCGCATGA ACACCCTGAC 
TCCAAGAAAA GATGCCACCT GTACTGTCAG TCCAAAGAGA CCGGGGACGT TGCCTACATG 
AAACAACTGG TGCATGACGG GACACGCTGC TCGTACAAAG ACCCGTACAG CATATGTGTG 
CGAGGAGAGT GTGTGAAAGT TGGCTGTGAT AAGGAAATCG GCTCCAACAA GGTTGAGGAT 
AAGTGTGGGG TCTGTGGAGG GGACAATTCC CACTGTCGAA CTGTGAAGGG GACATTTACC 
AGAACGCCCA GGAAACTTGG GAAGACGAGA GGCGCATTCG TTTTGCCCAA AGGAGCTCGT 
AATATCTCTC TTGCGGAAAC AAGAGAAACT AAAAATGTAC TGGCCATTAA GAACCAAGCC 
ACAGGTCACT ACATTTTAAA TGGCAAAGGG GAGGAAGCCA AGTCACGGAC CTTCATAGAC 
CTTGGTGTGG AGTGGGATTA CAACATTGAA GATGACATTG AAACTCTCCA CACGGATGGG 
CCACTACACG ACCCCGTTAT TGTTCTGATC ATACCTCAGG AGAACGACAC GCGCTCTAGC 
CTGACGTATA AGTACATCAT CCATGAAGAC TCCGTGCCAA CAATCAACAG TAACAACGTC 
ATCCAGGAGG AGCTGGACAC CTTCGAGTGG GCGCTGAAGA GCTGGTCTCA GTGCTCCAAA 
CCCTGTGGTG GAGGGTTCCA GTATACGAAA TACGGATGCC GAAGGAAAAG CGACAGTAAA 
ATGGTTCACC GCAGCTTCTG TGAGGTCAAT AAGAAGCCGA AACCCATCAG ACGCATGTGC 
AACATTCAGG AGTGCACACA CCCGCTCTGG GCAGCAGAAG CGTGGGAACA CTGCAGCAGA 
AGCTGTGGCA GCTCGGGGTA CCAGCTACGC ACCGTACGCT GCCTCCAACC ACTCCAGGAC 
GGTACCAACC GCTCAGTGCA CAGCAGGTAC TGCCTGGGAG AGCGCCCGGA GAGCCGCCGG 
CCCTGTAATA GAATACCCTG CCCAGCCCAG TGGAAAACAG GGCCCTGGAA TGAGTGTTCG 
GTGACCTGCG GTGAGGGAAC AGAGGTGAGA CAGGTTCTCT GTAGGACTGG AGACCACTGT 
GATGGGGAAA AGCCCGAGTC TGTCAGAGCC TGCCAGCTGC CTCCCTGTAA TGACGAGCCG 
TGCTTGGGCG ACAAGTCCAT ATTCTGTCAA ATGGAGGTGC TAGCAAGATA CTGCTCCATA 
CCAGGCTACA GCGAGCTGTG CTGCGAGTCA TGTAGCAAGC GCAGTGGCAC CCTGCCACCG 
CCATACCTTC AAGAAGCTGC TGAAATCCAC AGTGATGCCA TCTTTGGCCC TAGTGACCTC 
CCTAGGTCCC TAGTGATGCC CACACCCTTC CTCCCTTACT ACTCAGGGTC CCGTGCCGAC 
AAGAAATCCT TGAGTAGCGT CTCCTCCGTG GCAAATCCCA GCGCATATGC TGCTTTCAGA 
TCCAGCAGCA GAGCCAGTGT TAGCTTCCCC CGGAAGAAAG CACAAGAGGG TGGAACAAAG 
ACGCCGGTAT CAATGCCACC CTCCTCATCC ATAAAGACTG ATCACCTCCA TTCCTCTTCC 
CCTGGGGCTG CTGCCCCCTC CCCTGCTATC ACTGATTCCA TAGGTGCTTC TCCCCAGACA 
AGAACCCCAA AGAAAGATGG AAAGACTATT AGCAGGAGAC ATCCGATCAG GTCAACCGCC 
TCAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wellcome open research11(2016)
Wilson R,Geyer SH,Reissig L,Rose J,Szumska D,Hardman E,Prin F,McGuire C,Ramirez-Solis R,White J,Galli A,Tudor C,Tuck E,Mazzeo CI,Smith JC,Robertson E,Adams DJ,Mohun T,Weninger WJ

CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation.
Circulation129(19)1962-71(2014 May)
Jeltsch M,Jha SK,Tvorogov D,Anisimov A,Lepp?nen VM,Holopainen T,Kivel? R,Ortega S,K?rpanen T,Alitalo K

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

Regulation of procollagen amino-propeptide processing during mouse embryogenesis by specialization of homologous ADAMTS proteases: insights on collagen biosynthesis and dermatosparaxis.
Development (Cambridge, England)133(8)1587-96(2006 Apr)
Le Goff C,Somerville RP,Kesteloot F,Powell K,Birk DE,Colige AC,Apte SS

Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis.
The Journal of biological chemistry276(34)31502-9(2001 Aug)
Fernandes RJ,Hirohata S,Engle JM,Colige A,Cohn DH,Eyre DR,Apte SS

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Adamts3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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