Lrrc8d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrrc8d
Entrez Gene ID	305131
Full Name	leucine rich repeat containing 8 VRAC subunit D
Synonyms	Lrrc5
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit D Names volume-regulated anion channel subunit LRRC8D leucine rich repeat containing 8 family, member D leucine rich repeat containing 8D leucine-rich repeat-containing 5 leucine-rich repeat-containing protein 5 leucine-rich repeat-containing protein 8D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008338.1	NP_001008339.2	volume-regulated anion channel subunit LRRC8D
XM_017599156.1	XP_017454645.1	volume-regulated anion channel subunit LRRC8D isoform X1
XM_017599157.1	XP_017454646.1	volume-regulated anion channel subunit LRRC8D isoform X1
XM_017599159.1	XP_017454648.1	volume-regulated anion channel subunit LRRC8D isoform X1
XM_006250556.3	XP_006250618.1	volume-regulated anion channel subunit LRRC8D isoform X1
XM_017599158.1	XP_017454647.1	volume-regulated anion channel subunit LRRC8D isoform X1
XM_017599160.1	XP_017454649.1	volume-regulated anion channel subunit LRRC8D isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8D	NP_060573.2
Gallus gallus (chicken)	LRRC8D	XP_004936686.1
Xenopus tropicalis (tropical clawed frog)	lrrc8d	NP_001120353.1
Pan troglodytes (chimpanzee)	LRRC8D	XP_001150929.1
	LRRC8D	XP_002801674.1
Canis lupus familiaris (dog)	LRRC8D	XP_005622006.1
Bos taurus (cattle)	LRRC8D	NP_001077210.1
Mus musculus (house mouse)	Lrrc8d	NP_848816.3
Danio rerio (zebrafish)	lrrc8db	NP_001070848.1
Rattus norvegicus (Norway rat)	Lrrc8d	NP_001008339.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular composition and heterogeneity of the LRRC8-containing swelling-activated osmolyte channels in primary rat astrocytes.
Schober AL, Wilson CS, Mongin AA
The Journal of physiology595(22)6939-6951(2017 Nov)

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Lutter D, Ullrich F, Lueck JC, Kempa S, Jentsch TJ
Journal of cell science130(6)1122-1133(2017 03)

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Syeda R, Qiu Z, Dubin AE, Murthy SE, Florendo MN, Mason DE, Mathur J, Cahalan SM, Peters EC, Montal M, Patapoutian A
Cell164(3)499-511(2016 Jan)

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Voss FK, Ullrich F, M?nch J, Lazarow K, Lutter D, Mah N, Andrade-Navarro MA, von Kries JP, Stauber T, Jentsch TJ
Science (New York, N.Y.)344(6184)634-8(2014 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lrrc8d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrc8d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11574	NM_001008338.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 VRAC subunit D (Lrrc8d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
ORa35802	XM_017599159.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa35802	XM_017599156.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa35802	XM_017599160.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa35802	XM_006250556.3 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa35802	XM_017599158.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa35802	XM_017599157.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrrc8d ( NM_001008338.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008339.2 Rattus norvegicus leucine rich repeat containing 8 VRAC subunit D (Lrrc8d), mRNA.

Price & Availability↑

CloneID	ORa11574
Clone ID Related Accession (Same CDS sequence)	NM_001008338.1
Accession Version	NM_001008338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085783.1. On Dec 16, 2004 this sequence version replaced XM_223152.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC085783.1, CK481165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642765 [ECO:0000348] ##Evidence-Data-END##

ATGGGAATGT TTACCCTTGC GGAAGTTGCT TCACTTAATG ACATTCAGCC AACTTACCGA 
ATCCTGAAGC CATGGTGGGA CGTGTTTATG GATTACCTGG CGGTTGTTAT GCTGATGGTA 
GCCATCTTTG CAGGGACCAT GCAACTTACC AAAGATCAGG TGGTCTGTTT GCCAGTGTTG 
CCGTCGCCTG CAAATTCAAA GGCGCACACA CCACCCGGGA ATGCTGACGT CACCACTGAA 
GTCCCCAAGA TGGAGACAGC CACACCCCAA GACCAAAACG GGCAGACGAC GAATGACATT 
GCCTTTGGCA CATCTGCTGT GACGCCTGAC ATACCTCTCC AAGCCACCTA TTCACACGCA 
GAGTCCACCT TTCCCAGTCA GGAGACGAAG AAGGAGAAGA GAGACCCCAC GGGCCGCAAA 
ACCAACTTGG ATTTTCAGCA GTACGTATTT ATCAATCAGA TGTGCTACCA TCTGGCCCTT 
CCTTGGTATT CCAAGTACTT TCCATACCTT GCCCTTATAC ACACCATCAT CCTTATGGTC 
AGTAGCAACT TTTGGTTCAA ATATCCCAAA ACGTGCTCCA AGGTTGAGCA TTTTGTTTCA 
ATATTAGGAA AGTGTTTCGA ATCTCCCTGG ACTACTAAAG CGCTGTCCGA GACAGCCTGT 
GAGGACTCTG AGGAGAACAA GCAGCGGATA ACGGGCGCCC AGACCCTACC GAAGCACGTG 
TCCACCAGCA GCGATGAGGG GAGCCCCAGC GCCAGCACCC CCATGATCAA CAAAACGGGC 
TTCAAGTTCT CAGCTGAAAA GCCGGTGATC GAAGTTCCCA GCATGACGAT CCTGGACAAG 
AAGGACGGGG AGCAGGCCAA AGCCCTGTTT GAGAAGGTGA GGAAATTTCG TGCCCATGTG 
GAAGACAGTG ACTTGATCTA CAAGCTCTAC GTGGTCCAGA CCCTCATCAA GACCGCCAAG 
TTCATTTTTA TCCTCTGCTA CACTGCAAAC TTTGTCAACG CCATCAGCTT CGAGCATGTC 
TGCAAGCCAA AAGTCGAGCA CCTCACGGGG TACGAGGTGT TCGAGTGCAC TCACAATATG 
GCCTACATGT TGAAGAAACT GCTCATCAGC TACATCTCCA TCATCTGTGT CTATGGCTTC 
ATCTGCCTCT ACACTCTCTT CTGGCTGTTC AGGATCCCTC TGAAGGAATA TTCCTTTGAG 
AAAGTCCGAG AGGAAAGCAG CTTCAGCGAC ATCCCGGATG TCAAGAACGA CTTCGCGTTC 
CTCCTGCACA TGGTCGACCA GTACGACCAG CTCTATTCCA AGCGTTTTGG TGTCTTCTTA 
TCTGAAGTCA GTGAAAACAA ACTGAGGGAA ATCAGCCTGA ACCATGAGTG GACTTTCGAG 
AAACTCAGGC AGCACGTGTC TCGCAACGCC CAGGACAAGC AGGAGCTGCA CTTGTTCATG 
CTGTCAGGGG TGCCCGACGC TGTCTTTGAC CTCACGGACC TGGATGTGCT AAAACTTGAA 
CTGATTCCCG AAGCAAAAAT TCCTGCCAAG ATCTCTCAGA TGACGAACCT TCAAGAACTC 
CACCTCTGCC ACTGCCCTGC CAAGGTGGAG CAGACCGCCT TTAGCTTTCT TCGCGATCAC 
TTGAGATGCC TTCATGTGAA GTTCACTGAC GTGGCTGAAA TCCCTGCCTG GGTGTATTTG 
CTCAAAAACC TGCGGGAGCT GTACTTGATA GGCAACTTGA ACTCAGAGAA CAATAAGATG 
ATCGGGCTGG AGTCTCTGCG AGAGCTGCGG CACCTTAAGA TCCTCCACGT GAAGAGTAAC 
CTGACCAAAG TTCCCTCCAA CATAACTGAC GTGGCTCCTC ACCTCACCAA GCTCGTCATC 
CACAATGACG GCACCAAGCT CTTGGTGCTC AACAGCCTGA AGAAGATGAT GAACGTTGCC 
GAGCTAGAGC TGCAGAACTG TGAGCTGGAG AGGATTCCAC ACGCCATTTT CAGCCTTTCT 
AACTTGCAGG AGCTGGACCT GAAGTCCAAC AGCATCCGCA CGATCGAGGA GATCATCAGT 
TTCCAACACC TGAAGCGACT GACTTGCTTG AAATTGTGGC ACAATAAAAT TGTGGCCATT 
CCTCCCTCCA TTACCCACGT CAAGAACCTG GAGTCCCTCT ACTTCTCCAA CAACAAGCTC 
GAGTCCTTAC CGGTGGCAGT GTTTAGTTTA CAGAAACTCA GATGCTTAGA TGTCAGCTAT 
AACAACATTT CCACGATCCC CATAGAGATA GGTTTGCTTC AGAACCTGCA GCATTTGCAC 
ATCACAGGGA ACAAAGTGGA CGTTCTGCCA AAACAGTTGT TTAAGTGCGT GAAGTTGAGG 
ACTTTGAACC TGGGGCAGAA CTGCATCGCC TCCCTGCCTG AGAAGATCAG TCAGCTGTCC 
CAGCTCACTC AGCTGGAGCT GAAGGGCAAC TGTCTCGACC GCCTGCCAGC CCAGCTGGGC 
CAGTGTCGGA TGCTCAAGAA AAGCGGGCTT GTTGTAGAAG ACCAACTGTT TGACACGCTG 
CCACTGGAAG TCAAAGAGGC ATTGAATCAA GACGTAAATG TCCCCTTTGC GAACGGGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008339.2
CDS	171..2753
Translation	MGMFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	NM_001008338.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 VRAC subunit D (Lrrc8d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008338.1

ORF Insert Sequence:

ATGGGAATGT TTACCCTTGC GGAAGTTGCT TCACTTAATG ACATTCAGCC AACTTACCGA 
ATCCTGAAGC CATGGTGGGA CGTGTTTATG GATTACCTGG CGGTTGTTAT GCTGATGGTA 
GCCATCTTTG CAGGGACCAT GCAACTTACC AAAGATCAGG TGGTCTGTTT GCCAGTGTTG 
CCGTCGCCTG CAAATTCAAA GGCGCACACA CCACCCGGGA ATGCTGACGT CACCACTGAA 
GTCCCCAAGA TGGAGACAGC CACACCCCAA GACCAAAACG GGCAGACGAC GAATGACATT 
GCCTTTGGCA CATCTGCTGT GACGCCTGAC ATACCTCTCC AAGCCACCTA TTCACACGCA 
GAGTCCACCT TTCCCAGTCA GGAGACGAAG AAGGAGAAGA GAGACCCCAC GGGCCGCAAA 
ACCAACTTGG ATTTTCAGCA GTACGTATTT ATCAATCAGA TGTGCTACCA TCTGGCCCTT 
CCTTGGTATT CCAAGTACTT TCCATACCTT GCCCTTATAC ACACCATCAT CCTTATGGTC 
AGTAGCAACT TTTGGTTCAA ATATCCCAAA ACGTGCTCCA AGGTTGAGCA TTTTGTTTCA 
ATATTAGGAA AGTGTTTCGA ATCTCCCTGG ACTACTAAAG CGCTGTCCGA GACAGCCTGT 
GAGGACTCTG AGGAGAACAA GCAGCGGATA ACGGGCGCCC AGACCCTACC GAAGCACGTG 
TCCACCAGCA GCGATGAGGG GAGCCCCAGC GCCAGCACCC CCATGATCAA CAAAACGGGC 
TTCAAGTTCT CAGCTGAAAA GCCGGTGATC GAAGTTCCCA GCATGACGAT CCTGGACAAG 
AAGGACGGGG AGCAGGCCAA AGCCCTGTTT GAGAAGGTGA GGAAATTTCG TGCCCATGTG 
GAAGACAGTG ACTTGATCTA CAAGCTCTAC GTGGTCCAGA CCCTCATCAA GACCGCCAAG 
TTCATTTTTA TCCTCTGCTA CACTGCAAAC TTTGTCAACG CCATCAGCTT CGAGCATGTC 
TGCAAGCCAA AAGTCGAGCA CCTCACGGGG TACGAGGTGT TCGAGTGCAC TCACAATATG 
GCCTACATGT TGAAGAAACT GCTCATCAGC TACATCTCCA TCATCTGTGT CTATGGCTTC 
ATCTGCCTCT ACACTCTCTT CTGGCTGTTC AGGATCCCTC TGAAGGAATA TTCCTTTGAG 
AAAGTCCGAG AGGAAAGCAG CTTCAGCGAC ATCCCGGATG TCAAGAACGA CTTCGCGTTC 
CTCCTGCACA TGGTCGACCA GTACGACCAG CTCTATTCCA AGCGTTTTGG TGTCTTCTTA 
TCTGAAGTCA GTGAAAACAA ACTGAGGGAA ATCAGCCTGA ACCATGAGTG GACTTTCGAG 
AAACTCAGGC AGCACGTGTC TCGCAACGCC CAGGACAAGC AGGAGCTGCA CTTGTTCATG 
CTGTCAGGGG TGCCCGACGC TGTCTTTGAC CTCACGGACC TGGATGTGCT AAAACTTGAA 
CTGATTCCCG AAGCAAAAAT TCCTGCCAAG ATCTCTCAGA TGACGAACCT TCAAGAACTC 
CACCTCTGCC ACTGCCCTGC CAAGGTGGAG CAGACCGCCT TTAGCTTTCT TCGCGATCAC 
TTGAGATGCC TTCATGTGAA GTTCACTGAC GTGGCTGAAA TCCCTGCCTG GGTGTATTTG 
CTCAAAAACC TGCGGGAGCT GTACTTGATA GGCAACTTGA ACTCAGAGAA CAATAAGATG 
ATCGGGCTGG AGTCTCTGCG AGAGCTGCGG CACCTTAAGA TCCTCCACGT GAAGAGTAAC 
CTGACCAAAG TTCCCTCCAA CATAACTGAC GTGGCTCCTC ACCTCACCAA GCTCGTCATC 
CACAATGACG GCACCAAGCT CTTGGTGCTC AACAGCCTGA AGAAGATGAT GAACGTTGCC 
GAGCTAGAGC TGCAGAACTG TGAGCTGGAG AGGATTCCAC ACGCCATTTT CAGCCTTTCT 
AACTTGCAGG AGCTGGACCT GAAGTCCAAC AGCATCCGCA CGATCGAGGA GATCATCAGT 
TTCCAACACC TGAAGCGACT GACTTGCTTG AAATTGTGGC ACAATAAAAT TGTGGCCATT 
CCTCCCTCCA TTACCCACGT CAAGAACCTG GAGTCCCTCT ACTTCTCCAA CAACAAGCTC 
GAGTCCTTAC CGGTGGCAGT GTTTAGTTTA CAGAAACTCA GATGCTTAGA TGTCAGCTAT 
AACAACATTT CCACGATCCC CATAGAGATA GGTTTGCTTC AGAACCTGCA GCATTTGCAC 
ATCACAGGGA ACAAAGTGGA CGTTCTGCCA AAACAGTTGT TTAAGTGCGT GAAGTTGAGG 
ACTTTGAACC TGGGGCAGAA CTGCATCGCC TCCCTGCCTG AGAAGATCAG TCAGCTGTCC 
CAGCTCACTC AGCTGGAGCT GAAGGGCAAC TGTCTCGACC GCCTGCCAGC CCAGCTGGGC 
CAGTGTCGGA TGCTCAAGAA AAGCGGGCTT GTTGTAGAAG ACCAACTGTT TGACACGCTG 
CCACTGGAAG TCAAAGAGGC ATTGAATCAA GACGTAAATG TCCCCTTTGC GAACGGGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_017599156.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454645.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_017599156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454645.1
CDS	534..3110
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_017599156.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599156.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_017599157.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454646.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_017599157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454646.1
CDS	1195..3771
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_017599157.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599157.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_017599159.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454648.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_017599159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454648.1
CDS	1774..4350
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_017599159.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599159.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_006250556.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250618.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X1, mRNA.

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CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_006250556.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250618.1
CDS	228..2804
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_006250556.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250556.3

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_017599158.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454647.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_017599158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454647.1
CDS	241..2817
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_017599158.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599158.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8d ( XM_017599160.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454649.1 Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X6, mRNA.

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CloneID	ORa35802
Clone ID Related Accession (Same CDS sequence)	XM_017599156.1 , XM_017599157.1 , XM_017599159.1 , XM_006250556.3 , XM_017599158.1 , XM_017599160.1
Accession Version	XM_017599160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454649.1
CDS	765..3341
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLPKQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFANGI

Target ORF information:

RefSeq Version	XM_017599160.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member D (Lrrc8d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599160.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AGCCAACTTA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT TATGGATTAC CTGGCGGTTG TTATGCTGAT GGTAGCCATC 
TTTGCAGGGA CCATGCAACT TACCAAAGAT CAGGTGGTCT GTTTGCCAGT GTTGCCGTCG 
CCTGCAAATT CAAAGGCGCA CACACCACCC GGGAATGCTG ACGTCACCAC TGAAGTCCCC 
AAGATGGAGA CAGCCACACC CCAAGACCAA AACGGGCAGA CGACGAATGA CATTGCCTTT 
GGCACATCTG CTGTGACGCC TGACATACCT CTCCAAGCCA CCTATTCACA CGCAGAGTCC 
ACCTTTCCCA GTCAGGAGAC GAAGAAGGAG AAGAGAGACC CCACGGGCCG CAAAACCAAC 
TTGGATTTTC AGCAGTACGT ATTTATCAAT CAGATGTGCT ACCATCTGGC CCTTCCTTGG 
TATTCCAAGT ACTTTCCATA CCTTGCCCTT ATACACACCA TCATCCTTAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACGTGC TCCAAGGTTG AGCATTTTGT TTCAATATTA 
GGAAAGTGTT TCGAATCTCC CTGGACTACT AAAGCGCTGT CCGAGACAGC CTGTGAGGAC 
TCTGAGGAGA ACAAGCAGCG GATAACGGGC GCCCAGACCC TACCGAAGCA CGTGTCCACC 
AGCAGCGATG AGGGGAGCCC CAGCGCCAGC ACCCCCATGA TCAACAAAAC GGGCTTCAAG 
TTCTCAGCTG AAAAGCCGGT GATCGAAGTT CCCAGCATGA CGATCCTGGA CAAGAAGGAC 
GGGGAGCAGG CCAAAGCCCT GTTTGAGAAG GTGAGGAAAT TTCGTGCCCA TGTGGAAGAC 
AGTGACTTGA TCTACAAGCT CTACGTGGTC CAGACCCTCA TCAAGACCGC CAAGTTCATT 
TTTATCCTCT GCTACACTGC AAACTTTGTC AACGCCATCA GCTTCGAGCA TGTCTGCAAG 
CCAAAAGTCG AGCACCTCAC GGGGTACGAG GTGTTCGAGT GCACTCACAA TATGGCCTAC 
ATGTTGAAGA AACTGCTCAT CAGCTACATC TCCATCATCT GTGTCTATGG CTTCATCTGC 
CTCTACACTC TCTTCTGGCT GTTCAGGATC CCTCTGAAGG AATATTCCTT TGAGAAAGTC 
CGAGAGGAAA GCAGCTTCAG CGACATCCCG GATGTCAAGA ACGACTTCGC GTTCCTCCTG 
CACATGGTCG ACCAGTACGA CCAGCTCTAT TCCAAGCGTT TTGGTGTCTT CTTATCTGAA 
GTCAGTGAAA ACAAACTGAG GGAAATCAGC CTGAACCATG AGTGGACTTT CGAGAAACTC 
AGGCAGCACG TGTCTCGCAA CGCCCAGGAC AAGCAGGAGC TGCACTTGTT CATGCTGTCA 
GGGGTGCCCG ACGCTGTCTT TGACCTCACG GACCTGGATG TGCTAAAACT TGAACTGATT 
CCCGAAGCAA AAATTCCTGC CAAGATCTCT CAGATGACGA ACCTTCAAGA ACTCCACCTC 
TGCCACTGCC CTGCCAAGGT GGAGCAGACC GCCTTTAGCT TTCTTCGCGA TCACTTGAGA 
TGCCTTCATG TGAAGTTCAC TGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTGCGGG AGCTGTACTT GATAGGCAAC TTGAACTCAG AGAACAATAA GATGATCGGG 
CTGGAGTCTC TGCGAGAGCT GCGGCACCTT AAGATCCTCC ACGTGAAGAG TAACCTGACC 
AAAGTTCCCT CCAACATAAC TGACGTGGCT CCTCACCTCA CCAAGCTCGT CATCCACAAT 
GACGGCACCA AGCTCTTGGT GCTCAACAGC CTGAAGAAGA TGATGAACGT TGCCGAGCTA 
GAGCTGCAGA ACTGTGAGCT GGAGAGGATT CCACACGCCA TTTTCAGCCT TTCTAACTTG 
CAGGAGCTGG ACCTGAAGTC CAACAGCATC CGCACGATCG AGGAGATCAT CAGTTTCCAA 
CACCTGAAGC GACTGACTTG CTTGAAATTG TGGCACAATA AAATTGTGGC CATTCCTCCC 
TCCATTACCC ACGTCAAGAA CCTGGAGTCC CTCTACTTCT CCAACAACAA GCTCGAGTCC 
TTACCGGTGG CAGTGTTTAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTATAACAAC 
ATTTCCACGA TCCCCATAGA GATAGGTTTG CTTCAGAACC TGCAGCATTT GCACATCACA 
GGGAACAAAG TGGACGTTCT GCCAAAACAG TTGTTTAAGT GCGTGAAGTT GAGGACTTTG 
AACCTGGGGC AGAACTGCAT CGCCTCCCTG CCTGAGAAGA TCAGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG CAACTGTCTC GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGGATGCTCA AGAAAAGCGG GCTTGTTGTA GAAGACCAAC TGTTTGACAC GCTGCCACTG 
GAAGTCAAAG AGGCATTGAA TCAAGACGTA AATGTCCCCT TTGCGAACGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular composition and heterogeneity of the LRRC8-containing swelling-activated osmolyte channels in primary rat astrocytes.
The Journal of physiology595(22)6939-6951(2017 Nov)
Schober AL,Wilson CS,Mongin AA

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Journal of cell science130(6)1122-1133(2017 03)
Lutter D,Ullrich F,Lueck JC,Kempa S,Jentsch TJ

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Cell164(3)499-511(2016 Jan)
Syeda R,Qiu Z,Dubin AE,Murthy SE,Florendo MN,Mason DE,Mathur J,Cahalan SM,Peters EC,Montal M,Patapoutian A

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Science (New York, N.Y.)344(6184)634-8(2014 May)
Voss FK,Ullrich F,M?nch J,Lazarow K,Lutter D,Mah N,Andrade-Navarro MA,von Kries JP,Stauber T,Jentsch TJ

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Lrrc8d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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