Col9a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col9a1
Entrez Gene ID	305104
Full Name	collagen type IX alpha 1 chain
General protein information	Preferred Names collagen type IX alpha 1 chain Names collagen alpha-1(IX) chain collagen, type IX, alpha 1 procollagen, type IX, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100842.1	NP_001094312.1	collagen alpha-1(IX) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL9A1	XP_001136406.1
Canis lupus familiaris (dog)	COL9A1	XP_538994.3
Danio rerio (zebrafish)	col9a1	NP_998429.2
Rattus norvegicus (Norway rat)	Col9a1	NP_001094312.1
Mus musculus (house mouse)	Col9a1	NP_031766.3
Danio rerio (zebrafish)	zgc:194937	NP_001124096.1
	COL9A1	XP_001111640.1
Xenopus tropicalis (tropical clawed frog)	col9a1	XP_002939444.2
Homo sapiens (human)	COL9A1	NP_001842.3
Gallus gallus (chicken)	COL9A1	NP_001094381.1

Gene Ontology
Bibliography

Related articles in PubMed

Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen.
Posey KL, Hankenson K, Veerisetty AC, Bornstein P, Lawler J, Hecht JT
The American journal of pathology172(6)1664-74(2008 Jun)

The localization of proteins encoded by CRYM, KIAA1199, UBA52, COL9A3, and COL9A1, genes highly expressed in the cochlea.
Usami S, Takumi Y, Suzuki N, Oguchi T, Oshima A, Suzuki H, Kitoh R, Abe S, Sasaki A, Matsubara A
Neuroscience154(1)22-8(2008 Jun)

Ablation of collagen IX and COMP disrupts epiphyseal cartilage architecture.
Blumbach K, Niehoff A, Paulsson M, Zaucke F
Matrix biology : journal of the International Society for Matrix Biology27(4)306-18(2008 May)

A mutation in COL9A1 causes multiple epiphyseal dysplasia: further evidence for locus heterogeneity.
Czarny-Ratajczak M, Lohiniva J, Rogala P, Kozlowski K, Per?l? M, Carter L, Spector TD, Kolodziej L, Sepp?nen U, Glazar R, Kr?lewski J, Latos-Bielenska A, Ala-Kokko L
American journal of human genetics69(5)969-80(2001 Nov)

The role of collagen II and cartilage fibril-associated molecules in skeletal development.
Asz?di A, Hunziker EB, Olsen BR, F?ssler R
Osteoarthritis and cartilage9 Suppl AS150-9(2001)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collagen type IX alpha1 was found within the tectorial membrane as well as fibrocytes in the spiral ligament.
Title: The localization of proteins encoded by CRYM, KIAA1199, UBA52, COL9A3, and COL9A1, genes highly expressed in the cochlea.

The following Col9a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col9a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11138	NM_001100842.1 Latest version!	Rattus norvegicus collagen type IX alpha 1 chain (Col9a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col9a1 ( NM_001100842.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094312.1 Rattus norvegicus collagen type IX alpha 1 chain (Col9a1), mRNA.

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CloneID	ORa11138
Clone ID Related Accession (Same CDS sequence)	NM_001100842.1
Accession Version	NM_001100842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(IX) chain
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067099.1, AABR07067100.1, DY316977.1, DY313373.1 and BQ191679.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACTC TTATAAACCC AAGAGAACCC ACTGGGAAAA TGAAGAACTT CTGGAAAATT 
CCACTTTTCT TCTTTGCATG CAGTTTTCTG GGACCCTGTG TATCTGCAAC TCCTAAGCGT 
CGTGCAAGAT TTCCTGCCAA TTCTGTTTCT CATGGTGGAA GTGAACTGTG TCCCAAGATC 
AGGATTGGCC AGGATGACTT GCCAGGATTT GACCTGATTT CTCAGTTCCA AATTGAGAAG 
GCTGCATCTC GAAGGACTAT CCAGAGGGTG GTGGGGTCCA CAGCGTTACA AGTGGCTTAC 
AAGCTGGGAA GTAATGTAGA CTTCAGGATT CCAACAAGGC ATTTGTATCC CAGTGGACTG 
CCTGAAGAAT ATTCCTTTTT AACTACTTTC CGGATGACTG GAAGCACACT TGAAAAGCAA 
TGGAACATTT GGCAGATCCA GGATTCCTCA GGGAGGGAGC AAGTTGGCGT GAAGATAAAT 
GGCCAAACAA AGTCTGTCGC GTTTTCATAC AAGGGACTGG ACGGGAGTCT CCAAACGGCA 
GCCTTCTTGA ATTTGCAGTC CTTGTTTGAC TCCCGTTGGC ATAAGCTCAT GATTGGCGTG 
GAGAGAACAA GAGCTACTCT TTTTATTGAC TGCATCCAGA TCGATTCCTT ACCTATAAAG 
CCGAGAGCCC AGATCGATGC TGATGGCTTT GCTGTGCTGG GAAAACTTGT GGACAATCCT 
CAGGTTTCTG TTCCCTTTGA ACTCCAGTGG ATGCTGATTC ACTGTGACCC CCTGAGACCC 
CGGAGAGAGA CGTGCCATGA ACTGCCAGTC AGAATCACAA CCAGCCAGAC CACCGATGAG 
AGAGGTCCTC CGGGTGAGCA GGGGCCTCCG GGGCCTCCCG GGCCTCCTGG AGTTCCGGGC 
ATAGATGGTA TTGATGGTGA CCGAGGTCCA AAGGGTCCCC CCGGACCTCC GGGTCCTCCT 
GGAGACCCGG GCAAGCCAGG AGCACCAGGC AAGCCAGGCA CTCCAGGAGC TGATGGATTA 
ACAGGACCTG ATGGGTCCCC GGGCTCCGTT GGACCAAGGG GACAAAAAGG AGAACCTGGT 
GTGCCTGGGT CTCGTGGATT TCCAGGCCGT GGCATTCCAG GACCCCCTGG CCCTCCTGGG 
ACCACAGGAC TTCCTGGAGA ACTTGGCCGA GTAGGCCCTG CTGGCGACCC TGGGAAAAGA 
GGACCACCTG GTCCTCCTGG ACCCCCAGGA CCTAGTGGAA CAATTGGATT TCATGATGGA 
GACCCATTGT GCCCCAATTC CTGCCCACCA GGTCGCTCTG GCTATCCAGG CCTACCAGGC 
ATGAGAGGCC ACAAAGGGGC GAAAGGAGAA ATTGGCGAGC CAGGAAGACA AGGACACAAG 
GGTGAAGAGG GTGACCAGGG GGAACTGGGA GAAGTTGGAG CTCAAGGACC TCCAGGAGCT 
CAAGGTCTGA GAGGCATCAC TGGCATAGTT GGAGACAAAG GAGAAAAAGG TGCTCGGGGA 
TTCGATGGAG ATCCTGGGCC TCAGGGCGTT CCAGGTGCAG CTGGTGATCA AGGGCAGCGA 
GGACCTCCAG GAGAAACAGG TCCTAAGGGA GACAGGGGCA TTCAAGGTTC CCGAGGAATT 
CCTGGATCCC CGGGTCCAAA AGGAGACACG GGCTTGCCAG GTGTCGATGG TCGAGACGGA 
ATCCCGGGAA TGCCTGGAAC AAAGGGTGAA GCAGGAAAGC CTGGACCTCC CGGTGATGCG 
GGATTGCAGG GTTTACCGGG TGTACCTGGG ATTCCTGGTG CAAAAGGTGT GGCCGGCGAA 
AAGGGTAACA CGGGTCCTCC GGGGAAGCCT GGTCAGTTGG GAAGTTCAGG AAAACCAGGC 
CAACAAGGAC CGCCGGGAGA GGTTGGACCT CGGGGACCCA GGGGCCTTCC AGGCAGCAGA 
GGCCCGGTAG GACCAGAAGG CTCTCCAGGC ATACCAGGGA AACTGGGACC TCTTGGCAGC 
CCTGGCCTTC CTGGCTTGCC TGGGCCCCCT GGACTTCCTG GAATGAAAGG AGACAGGGGT 
GTATTTGGTG AACCAGGTCC CAAGGGCGAA CAGGGTGCCT CTGGTGAAGA AGGTGAAGCA 
GGAGTAAGGG GTGACCTTGG AGATATGGGA CAACCTGGCC CAAAGGGATC AGTGGGTAAC 
CCTGGGGAGC CCGGCCTGAG GGGACCTGAA GGAATCAGAG GGCTTCCTGG AGTGGAAGGA 
CCAAGAGGAC CACCTGGACC CCGAGGTGTG CAAGGAGAAC AGGGTGCCAC TGGACTGCCT 
GGTATCCAGG GACCTCCGGG CAGAGCGCCG ACGGACCAGC ACATCAAGCA GGTTTGCATG 
AGAGTTGTGC AAGAGCATTT TGCAGAAATG GCAGCTAGCC TCAAGAGACC AGACACAGGA 
GCTTCTGGTC TTCCTGGGAG GCCTGGTCCC CCAGGGCCTC CCGGCCCTCC AGGAGAGAAT 
GGTTTCCCTG GTCAGATGGG AATCCGTGGC CTTCCAGGCA TTAAGGGTCC CCCTGGTGCT 
CTTGGCTTAA GGGGACCTAA AGGTGACTTG GGAGAAAAAG GAGAACGTGG TCCTCCAGGA 
AGAGGTCCTA AGGGTTTGCC TGGAGCTATC GGTCTCCCAG GTGATCCAGG CCCTGCCAGC 
TATGGGAAAA ATGGCCGAGA TGGAGAGCAA GGTCCCCCAG GAGTGGCAGG AATTCCTGGT 
GTGCCTGGGC CCCCAGGTCC TCCAGGCCCT CCTGGTTTCT GTGAGCCAGC CTCTTGCACC 
CTTCAGGCAG GTCAAAGAGC ATTTAGCAAA GGGCCTGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094312.1
CDS	207..3011
Translation	MVTLINPREPTGKMKNFWKIPLFFFACSFLGPCVSATPKRRARFPANSVSHGGSELCPKIRIGQDDLPGFDLISQFQIEKAASRRTIQRVVGSTALQVAYKLGSNVDFRIPTRHLYPSGLPEEYSFLTTFRMTGSTLEKQWNIWQIQDSSGREQVGVKINGQTKSVAFSYKGLDGSLQTAAFLNLQSLFDSRWHKLMIGVERTRATLFIDCIQIDSLPIKPRAQIDADGFAVLGKLVDNPQVSVPFELQWMLIHCDPLRPRRETCHELPVRITTSQTTDERGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPPGDPGKPGAPGKPGTPGADGLTGPDGSPGSVGPRGQKGEPGVPGSRGFPGRGIPGPPGPPGTTGLPGELGRVGPAGDPGKRGPPGPPGPPGPSGTIGFHDGDPLCPNSCPPGRSGYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQGELGEVGAQGPPGAQGLRGITGIVGDKGEKGARGFDGDPGPQGVPGAAGDQGQRGPPGETGPKGDRGIQGSRGIPGSPGPKGDTGLPGVDGRDGIPGMPGTKGEAGKPGPPGDAGLQGLPGVPGIPGAKGVAGEKGNTGPPGKPGQLGSSGKPGQQGPPGEVGPRGPRGLPGSRGPVGPEGSPGIPGKLGPLGSPGLPGLPGPPGLPGMKGDRGVFGEPGPKGEQGASGEEGEAGVRGDLGDMGQPGPKGSVGNPGEPGLRGPEGIRGLPGVEGPRGPPGPRGVQGEQGATGLPGIQGPPGRAPTDQHIKQVCMRVVQEHFAEMAASLKRPDTGASGLPGRPGPPGPPGPPGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEKGERGPPGRGPKGLPGAIGLPGDPGPASYGKNGRDGEQGPPGVAGIPGVPGPPGPPGPPGFCEPASCTLQAGQRAFSKGPDK

Target ORF information:

RefSeq Version	NM_001100842.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IX alpha 1 chain (Col9a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100842.1

ORF Insert Sequence:

ATGGTGACTC TTATAAACCC AAGAGAACCC ACTGGGAAAA TGAAGAACTT CTGGAAAATT 
CCACTTTTCT TCTTTGCATG CAGTTTTCTG GGACCCTGTG TATCTGCAAC TCCTAAGCGT 
CGTGCAAGAT TTCCTGCCAA TTCTGTTTCT CATGGTGGAA GTGAACTGTG TCCCAAGATC 
AGGATTGGCC AGGATGACTT GCCAGGATTT GACCTGATTT CTCAGTTCCA AATTGAGAAG 
GCTGCATCTC GAAGGACTAT CCAGAGGGTG GTGGGGTCCA CAGCGTTACA AGTGGCTTAC 
AAGCTGGGAA GTAATGTAGA CTTCAGGATT CCAACAAGGC ATTTGTATCC CAGTGGACTG 
CCTGAAGAAT ATTCCTTTTT AACTACTTTC CGGATGACTG GAAGCACACT TGAAAAGCAA 
TGGAACATTT GGCAGATCCA GGATTCCTCA GGGAGGGAGC AAGTTGGCGT GAAGATAAAT 
GGCCAAACAA AGTCTGTCGC GTTTTCATAC AAGGGACTGG ACGGGAGTCT CCAAACGGCA 
GCCTTCTTGA ATTTGCAGTC CTTGTTTGAC TCCCGTTGGC ATAAGCTCAT GATTGGCGTG 
GAGAGAACAA GAGCTACTCT TTTTATTGAC TGCATCCAGA TCGATTCCTT ACCTATAAAG 
CCGAGAGCCC AGATCGATGC TGATGGCTTT GCTGTGCTGG GAAAACTTGT GGACAATCCT 
CAGGTTTCTG TTCCCTTTGA ACTCCAGTGG ATGCTGATTC ACTGTGACCC CCTGAGACCC 
CGGAGAGAGA CGTGCCATGA ACTGCCAGTC AGAATCACAA CCAGCCAGAC CACCGATGAG 
AGAGGTCCTC CGGGTGAGCA GGGGCCTCCG GGGCCTCCCG GGCCTCCTGG AGTTCCGGGC 
ATAGATGGTA TTGATGGTGA CCGAGGTCCA AAGGGTCCCC CCGGACCTCC GGGTCCTCCT 
GGAGACCCGG GCAAGCCAGG AGCACCAGGC AAGCCAGGCA CTCCAGGAGC TGATGGATTA 
ACAGGACCTG ATGGGTCCCC GGGCTCCGTT GGACCAAGGG GACAAAAAGG AGAACCTGGT 
GTGCCTGGGT CTCGTGGATT TCCAGGCCGT GGCATTCCAG GACCCCCTGG CCCTCCTGGG 
ACCACAGGAC TTCCTGGAGA ACTTGGCCGA GTAGGCCCTG CTGGCGACCC TGGGAAAAGA 
GGACCACCTG GTCCTCCTGG ACCCCCAGGA CCTAGTGGAA CAATTGGATT TCATGATGGA 
GACCCATTGT GCCCCAATTC CTGCCCACCA GGTCGCTCTG GCTATCCAGG CCTACCAGGC 
ATGAGAGGCC ACAAAGGGGC GAAAGGAGAA ATTGGCGAGC CAGGAAGACA AGGACACAAG 
GGTGAAGAGG GTGACCAGGG GGAACTGGGA GAAGTTGGAG CTCAAGGACC TCCAGGAGCT 
CAAGGTCTGA GAGGCATCAC TGGCATAGTT GGAGACAAAG GAGAAAAAGG TGCTCGGGGA 
TTCGATGGAG ATCCTGGGCC TCAGGGCGTT CCAGGTGCAG CTGGTGATCA AGGGCAGCGA 
GGACCTCCAG GAGAAACAGG TCCTAAGGGA GACAGGGGCA TTCAAGGTTC CCGAGGAATT 
CCTGGATCCC CGGGTCCAAA AGGAGACACG GGCTTGCCAG GTGTCGATGG TCGAGACGGA 
ATCCCGGGAA TGCCTGGAAC AAAGGGTGAA GCAGGAAAGC CTGGACCTCC CGGTGATGCG 
GGATTGCAGG GTTTACCGGG TGTACCTGGG ATTCCTGGTG CAAAAGGTGT GGCCGGCGAA 
AAGGGTAACA CGGGTCCTCC GGGGAAGCCT GGTCAGTTGG GAAGTTCAGG AAAACCAGGC 
CAACAAGGAC CGCCGGGAGA GGTTGGACCT CGGGGACCCA GGGGCCTTCC AGGCAGCAGA 
GGCCCGGTAG GACCAGAAGG CTCTCCAGGC ATACCAGGGA AACTGGGACC TCTTGGCAGC 
CCTGGCCTTC CTGGCTTGCC TGGGCCCCCT GGACTTCCTG GAATGAAAGG AGACAGGGGT 
GTATTTGGTG AACCAGGTCC CAAGGGCGAA CAGGGTGCCT CTGGTGAAGA AGGTGAAGCA 
GGAGTAAGGG GTGACCTTGG AGATATGGGA CAACCTGGCC CAAAGGGATC AGTGGGTAAC 
CCTGGGGAGC CCGGCCTGAG GGGACCTGAA GGAATCAGAG GGCTTCCTGG AGTGGAAGGA 
CCAAGAGGAC CACCTGGACC CCGAGGTGTG CAAGGAGAAC AGGGTGCCAC TGGACTGCCT 
GGTATCCAGG GACCTCCGGG CAGAGCGCCG ACGGACCAGC ACATCAAGCA GGTTTGCATG 
AGAGTTGTGC AAGAGCATTT TGCAGAAATG GCAGCTAGCC TCAAGAGACC AGACACAGGA 
GCTTCTGGTC TTCCTGGGAG GCCTGGTCCC CCAGGGCCTC CCGGCCCTCC AGGAGAGAAT 
GGTTTCCCTG GTCAGATGGG AATCCGTGGC CTTCCAGGCA TTAAGGGTCC CCCTGGTGCT 
CTTGGCTTAA GGGGACCTAA AGGTGACTTG GGAGAAAAAG GAGAACGTGG TCCTCCAGGA 
AGAGGTCCTA AGGGTTTGCC TGGAGCTATC GGTCTCCCAG GTGATCCAGG CCCTGCCAGC 
TATGGGAAAA ATGGCCGAGA TGGAGAGCAA GGTCCCCCAG GAGTGGCAGG AATTCCTGGT 
GTGCCTGGGC CCCCAGGTCC TCCAGGCCCT CCTGGTTTCT GTGAGCCAGC CTCTTGCACC 
CTTCAGGCAG GTCAAAGAGC ATTTAGCAAA GGGCCTGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen.
The American journal of pathology172(6)1664-74(2008 Jun)
Posey KL,Hankenson K,Veerisetty AC,Bornstein P,Lawler J,Hecht JT

The localization of proteins encoded by CRYM, KIAA1199, UBA52, COL9A3, and COL9A1, genes highly expressed in the cochlea.
Neuroscience154(1)22-8(2008 Jun)
Usami S,Takumi Y,Suzuki N,Oguchi T,Oshima A,Suzuki H,Kitoh R,Abe S,Sasaki A,Matsubara A

Ablation of collagen IX and COMP disrupts epiphyseal cartilage architecture.
Matrix biology : journal of the International Society for Matrix Biology27(4)306-18(2008 May)
Blumbach K,Niehoff A,Paulsson M,Zaucke F

A mutation in COL9A1 causes multiple epiphyseal dysplasia: further evidence for locus heterogeneity.
American journal of human genetics69(5)969-80(2001 Nov)
Czarny-Ratajczak M,Lohiniva J,Rogala P,Kozlowski K,Per?l? M,Carter L,Spector TD,Kolodziej L,Sepp?nen U,Glazar R,Kr?lewski J,Latos-Bielenska A,Ala-Kokko L

The role of collagen II and cartilage fibril-associated molecules in skeletal development.
Osteoarthritis and cartilage9 Suppl AS150-9(2001)
Asz?di A,Hunziker EB,Olsen BR,F?ssler R

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Col9a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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