Klf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klf1
Entrez Gene ID	304666
Full Name	Kruppel like factor 1
General protein information	Preferred Names Kruppel like factor 1 Names Krueppel-like factor 1 Kruppel-like factor 1 (erythroid) erythroid Kruppel-like factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255254.3	XP_006255316.1	Krueppel-like factor 1 isoform X1
NM_001107164.1	NP_001100634.1	Krueppel-like factor 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KLF1	NP_001073828.1
Homo sapiens (human)	KLF1	NP_006554.1
	KLF1	XP_001109612.1
Rattus norvegicus (Norway rat)	Klf1	NP_001100634.1
Pan troglodytes (chimpanzee)	KLF1	XP_524128.1
Canis lupus familiaris (dog)	KLF1	XP_542040.3
Mus musculus (house mouse)	Klf1	NP_034765.2

Gene Ontology
Bibliography

Related articles in PubMed

Compound heterozygosity for KLF1 mutations is associated with microcytic hypochromic anemia and increased fetal hemoglobin.
Huang J, Zhang X, Liu D, Wei X, Shang X, Xiong F, Yu L, Yin X, Xu X
European journal of human genetics : EJHG23(10)1341-8(2015 Oct)

Transcription factors Fli1 and EKLF in the differentiation of megakaryocytic and erythroid progenitor in 5q- syndrome and in Diamond-Blackfan anemia.
Neuwirtova R, Fuchs O, Holicka M, Vostry M, Kostecka A, Hajkova H, Jonasova A, Cermak J, Cmejla R, Pospisilova D, Belickova M, Siskova M, Hochova I, Vondrakova J, Sponerova D, Kadlckova E, Novakova L, Brezinova J, Michalova K
Annals of hematology92(1)11-8(2013 Jan)

Kr?ppel-like Factor 4 activates HBG gene expression in primary erythroid cells.
Kalra IS, Alam MM, Choudhary PK, Pace BS
British journal of haematology154(2)248-59(2011 Jul)

Mutation in erythroid specific transcription factor KLF1 causes Hereditary Spherocytosis in the Nan hemolytic anemia mouse model.
Heruth DP, Hawkins T, Logsdon DP, Gibson MI, Sokolovsky IV, Nsumu NN, Major SL, Fegley B, Woods GM, Lewing KB, Neville KA, Cornetta K, Peterson KR, White RA
Genomics96(5)303-7(2010 Nov)

A dominant mutation in the gene encoding the erythroid transcription factor KLF1 causes a congenital dyserythropoietic anemia.
Arnaud L, Saison C, Helias V, Lucien N, Steschenko D, Giarratana MC, Prehu C, Foliguet B, Montout L, de Brevern AG, Francina A, Ripoche P, Fenneteau O, Da Costa L, Peyrard T, Coghlan G, Illum N, Birgens H, Tamary H, Iolascon A, Delaunay J, Tchernia G, Cartron JP
American journal of human genetics87(5)721-7(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Klf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03012	NM_001107164.1 Latest version!	Rattus norvegicus Kruppel like factor 1 (Klf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa38703	XM_006255254.3 Latest version!	Rattus norvegicus Kruppel like factor 1 (Klf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klf1 ( NM_001107164.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100634.1 Rattus norvegicus Kruppel like factor 1 (Klf1), mRNA.

Price & Availability↑

CloneID	ORa03012
Clone ID Related Accession (Same CDS sequence)	NM_001107164.1
Accession Version	NM_001107164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	Krueppel-like factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Oct 4, 2007 this sequence version replaced XM_001071193.1, XM_222473.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ232766.1, FQ226533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCAGA AGAGAGAGAG GAGGCCTGAG GCCCAGGGTG GACAGCAGCC AGTCATGGCC 
TCAGCTGAGA CTGTCTTACC CTCCATCAGT ACACTCACCA CCCTGGGACA GTTCCTGGAC 
ACCCAGGAGG ACTTCCTCAA GTGGTGGCGG TCTGAGGAGA CACAGGATTT GGGCCCGGGA 
CCCCCGAATC CCACGGAACC GTCCTTTCAC GTGAGTCTGA AATCAGAGGA CCCTCCCGGA 
GAGGACGATG AGAGGGACGT GACCTGTGCG TGGGATCCGG ATCTTTTCCT TACAAACTTT 
TCAGGTCCCG AGCCGCCCAG CACTCCCCGG ACCTGTGCCC TGGCACCCAG CGGGGGCCCA 
GTGGCACAGT TCGCGCCGCC CGAGTCTCTG AGCGCTTATG CGGGTGGCCC AGGGTTGGTG 
ACCGGGCCTT TGGGCTCCGA GGAGCACGCA GGCTGGGCGC ACCCGACTCC GAGACCCCCA 
ACCCCTGAGC CCTTCGTGGC CCCTGTCCTG GCCCCGGGAC TCGCTCCCAA GGCGCAGCCC 
GCATTCTCCG AATCGCGAGC GGTCTCGGCC GGAGGTTTCT TCCCGCGAGC GGGGCTTGCG 
GTGCCCGCAG CTCCAAGCGC CCCCTATGGG CTGCTGTCGG GATACCCCGC GCTCTACCCG 
GCGCCTCAGT ACCAAGGCCA TTTCCAGCTC TTTCGTGGGC TCGCGGCGCC TTCTGCTGGT 
CCCACTGCGC CCCCTTCCTT TTTGAATTGT CTGGGACCTG GTGCGGTGGG CACAGAACTC 
GGGGCCACTG CGATCGCCGG AGACGCAGGC TTGTCCCCAG GAGCTGCGCC GCCCAAACGC 
AGCCGGCGAA CTCTGGCACA AAAGAGGCAG GCGGCACACA CCTGCGGGCA CGAAGGATGC 
GGGAAGAGCT ACACCAAGAG CTCGCACCTG AAGGCGCACC TGCGCACGCA CACCGGAGAG 
AAGCCTTATG CCTGCTCCTG GGATGGCTGC AACTGGAGGT TCGCTCGCTC TGACGAACTG 
ACGCGCCACT ACCGGAAGCA CACTGGACAT CGCCCCTTCT GCTGTGGCCT CTGCCCACGT 
GCTTTCTCAC GCTCCGACCA CCTAGCTCTT CACATGAAGC GTCACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100634.1
CDS	48..1178
Translation	MRQKRERRPEAQGGQQPVMASAETVLPSISTLTTLGQFLDTQEDFLKWWRSEETQDLGPGPPNPTEPSFHVSLKSEDPPGEDDERDVTCAWDPDLFLTNFSGPEPPSTPRTCALAPSGGPVAQFAPPESLSAYAGGPGLVTGPLGSEEHAGWAHPTPRPPTPEPFVAPVLAPGLAPKAQPAFSESRAVSAGGFFPRAGLAVPAAPSAPYGLLSGYPALYPAPQYQGHFQLFRGLAAPSAGPTAPPSFLNCLGPGAVGTELGATAIAGDAGLSPGAAPPKRSRRTLAQKRQAAHTCGHEGCGKSYTKSSHLKAHLRTHTGEKPYACSWDGCNWRFARSDELTRHYRKHTGHRPFCCGLCPRAFSRSDHLALHMKRHL

Target ORF information:

RefSeq Version	NM_001107164.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kruppel like factor 1 (Klf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107164.1

ORF Insert Sequence:

ATGAGGCAGA AGAGAGAGAG GAGGCCTGAG GCCCAGGGTG GACAGCAGCC AGTCATGGCC 
TCAGCTGAGA CTGTCTTACC CTCCATCAGT ACACTCACCA CCCTGGGACA GTTCCTGGAC 
ACCCAGGAGG ACTTCCTCAA GTGGTGGCGG TCTGAGGAGA CACAGGATTT GGGCCCGGGA 
CCCCCGAATC CCACGGAACC GTCCTTTCAC GTGAGTCTGA AATCAGAGGA CCCTCCCGGA 
GAGGACGATG AGAGGGACGT GACCTGTGCG TGGGATCCGG ATCTTTTCCT TACAAACTTT 
TCAGGTCCCG AGCCGCCCAG CACTCCCCGG ACCTGTGCCC TGGCACCCAG CGGGGGCCCA 
GTGGCACAGT TCGCGCCGCC CGAGTCTCTG AGCGCTTATG CGGGTGGCCC AGGGTTGGTG 
ACCGGGCCTT TGGGCTCCGA GGAGCACGCA GGCTGGGCGC ACCCGACTCC GAGACCCCCA 
ACCCCTGAGC CCTTCGTGGC CCCTGTCCTG GCCCCGGGAC TCGCTCCCAA GGCGCAGCCC 
GCATTCTCCG AATCGCGAGC GGTCTCGGCC GGAGGTTTCT TCCCGCGAGC GGGGCTTGCG 
GTGCCCGCAG CTCCAAGCGC CCCCTATGGG CTGCTGTCGG GATACCCCGC GCTCTACCCG 
GCGCCTCAGT ACCAAGGCCA TTTCCAGCTC TTTCGTGGGC TCGCGGCGCC TTCTGCTGGT 
CCCACTGCGC CCCCTTCCTT TTTGAATTGT CTGGGACCTG GTGCGGTGGG CACAGAACTC 
GGGGCCACTG CGATCGCCGG AGACGCAGGC TTGTCCCCAG GAGCTGCGCC GCCCAAACGC 
AGCCGGCGAA CTCTGGCACA AAAGAGGCAG GCGGCACACA CCTGCGGGCA CGAAGGATGC 
GGGAAGAGCT ACACCAAGAG CTCGCACCTG AAGGCGCACC TGCGCACGCA CACCGGAGAG 
AAGCCTTATG CCTGCTCCTG GGATGGCTGC AACTGGAGGT TCGCTCGCTC TGACGAACTG 
ACGCGCCACT ACCGGAAGCA CACTGGACAT CGCCCCTTCT GCTGTGGCCT CTGCCCACGT 
GCTTTCTCAC GCTCCGACCA CCTAGCTCTT CACATGAAGC GTCACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klf1 ( XM_006255254.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255316.1 Rattus norvegicus Kruppel like factor 1 (Klf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38703
Clone ID Related Accession (Same CDS sequence)	XM_006255254.3
Accession Version	XM_006255254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Krueppel-like factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCAGA AGAGAGAGAG GAGGCCTGAG GCCCAGGGTG GACAGCAGCC AGTCATGGCC 
TCAGCTGAGA CTGTCTTACC CTCCATCAGT ACACTCACCA CCCTGGGACA GTTCCTGGAC 
ACCCAGGAGG ACTTCCTCAA GTGGTGGCGG TCTGAGGAGA CACAGGATTT GGGCCCGGGA 
CCCCCGAATC CCACGGAACC GTCCTTTCAC GTGAGTCTGA AATCAGAGGA CCCTCCCGGA 
GAGGACGATG AGAGGGACGT GACCTGTGCG TGGGATCCGG ATCTTTTCCT TACAAACTTT 
TCAGGTCCCG AGCCGCCCAG CACTCCCCGG ACCTGTGCCC TGGCACCCAG CGGGGGCCCA 
GTGGCACAGT TCGCGCCGCC CGAGTCTCTG AGCGCTTATG CGGGTGGCCC AGGGTTGGTG 
ACCGGGCCTT TGGGCTCCGA GGAGCACGCA GGCTGGGCGC ACCCGACTCC GAGACCCCCA 
ACCCCTGAGC CCTTCGTGGC CCCTGTCCTG GCCCCGGGAC TCGCTCCCAA GGCGCAGCCC 
GCATTCTCCG AATCGCGAGC GGTCTCGGCC GGAGGTTTCT TCCCGCGAGC GGGGCTTGCG 
GTGCCCGCAG CTCCAAGCGC CCCCTATGGG CTGCTGTCGG GATACCCCGC GCTCTACCCG 
GCGCCTCAGT ACCAAGGCCA TTTCCAGCTC TTTCGTGGGC TCGCGGCGCC TTCTGCTGGT 
CCCACTGCGC CCCCTTCCTT TTTGAATTGT CTGGGACCTG GTGCGGTGGG CACAGAACTC 
GGGGCCACTG CGATCGCCGG AGACGCAGGC TTGTCCCCAG GAGCTGCGCC GCCCAAACGC 
AGCCGGCGAA CTCTGGCACA AAAGAGGCAG GCGGCACACA CCTGCGGGCA CGAAGGATGC 
GGGAAGAGCT ACACCAAGAG CTCGCACCTG AAGGCGCACC TGCGCACGCA CACCGCTCCC 
TGTCCTTCAG GAGAGAAGCC TTATGCCTGC TCCTGGGATG GCTGCAACTG GAGGTTCGCT 
CGCTCTGACG AACTGACGCG CCACTACCGG AAGCACACTG GACATCGCCC CTTCTGCTGT 
GGCCTCTGCC CACGTGCTTT CTCACGCTCC GACCACCTAG CTCTTCACAT GAAGCGTCAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255316.1
CDS	141..1286
Translation	MRQKRERRPEAQGGQQPVMASAETVLPSISTLTTLGQFLDTQEDFLKWWRSEETQDLGPGPPNPTEPSFHVSLKSEDPPGEDDERDVTCAWDPDLFLTNFSGPEPPSTPRTCALAPSGGPVAQFAPPESLSAYAGGPGLVTGPLGSEEHAGWAHPTPRPPTPEPFVAPVLAPGLAPKAQPAFSESRAVSAGGFFPRAGLAVPAAPSAPYGLLSGYPALYPAPQYQGHFQLFRGLAAPSAGPTAPPSFLNCLGPGAVGTELGATAIAGDAGLSPGAAPPKRSRRTLAQKRQAAHTCGHEGCGKSYTKSSHLKAHLRTHTAPCPSGEKPYACSWDGCNWRFARSDELTRHYRKHTGHRPFCCGLCPRAFSRSDHLALHMKRHL

Target ORF information:

RefSeq Version	XM_006255254.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kruppel like factor 1 (Klf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255254.3

ORF Insert Sequence:

ATGAGGCAGA AGAGAGAGAG GAGGCCTGAG GCCCAGGGTG GACAGCAGCC AGTCATGGCC 
TCAGCTGAGA CTGTCTTACC CTCCATCAGT ACACTCACCA CCCTGGGACA GTTCCTGGAC 
ACCCAGGAGG ACTTCCTCAA GTGGTGGCGG TCTGAGGAGA CACAGGATTT GGGCCCGGGA 
CCCCCGAATC CCACGGAACC GTCCTTTCAC GTGAGTCTGA AATCAGAGGA CCCTCCCGGA 
GAGGACGATG AGAGGGACGT GACCTGTGCG TGGGATCCGG ATCTTTTCCT TACAAACTTT 
TCAGGTCCCG AGCCGCCCAG CACTCCCCGG ACCTGTGCCC TGGCACCCAG CGGGGGCCCA 
GTGGCACAGT TCGCGCCGCC CGAGTCTCTG AGCGCTTATG CGGGTGGCCC AGGGTTGGTG 
ACCGGGCCTT TGGGCTCCGA GGAGCACGCA GGCTGGGCGC ACCCGACTCC GAGACCCCCA 
ACCCCTGAGC CCTTCGTGGC CCCTGTCCTG GCCCCGGGAC TCGCTCCCAA GGCGCAGCCC 
GCATTCTCCG AATCGCGAGC GGTCTCGGCC GGAGGTTTCT TCCCGCGAGC GGGGCTTGCG 
GTGCCCGCAG CTCCAAGCGC CCCCTATGGG CTGCTGTCGG GATACCCCGC GCTCTACCCG 
GCGCCTCAGT ACCAAGGCCA TTTCCAGCTC TTTCGTGGGC TCGCGGCGCC TTCTGCTGGT 
CCCACTGCGC CCCCTTCCTT TTTGAATTGT CTGGGACCTG GTGCGGTGGG CACAGAACTC 
GGGGCCACTG CGATCGCCGG AGACGCAGGC TTGTCCCCAG GAGCTGCGCC GCCCAAACGC 
AGCCGGCGAA CTCTGGCACA AAAGAGGCAG GCGGCACACA CCTGCGGGCA CGAAGGATGC 
GGGAAGAGCT ACACCAAGAG CTCGCACCTG AAGGCGCACC TGCGCACGCA CACCGCTCCC 
TGTCCTTCAG GAGAGAAGCC TTATGCCTGC TCCTGGGATG GCTGCAACTG GAGGTTCGCT 
CGCTCTGACG AACTGACGCG CCACTACCGG AAGCACACTG GACATCGCCC CTTCTGCTGT 
GGCCTCTGCC CACGTGCTTT CTCACGCTCC GACCACCTAG CTCTTCACAT GAAGCGTCAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compound heterozygosity for KLF1 mutations is associated with microcytic hypochromic anemia and increased fetal hemoglobin.
European journal of human genetics : EJHG23(10)1341-8(2015 Oct)
Huang J,Zhang X,Liu D,Wei X,Shang X,Xiong F,Yu L,Yin X,Xu X

Transcription factors Fli1 and EKLF in the differentiation of megakaryocytic and erythroid progenitor in 5q- syndrome and in Diamond-Blackfan anemia.
Annals of hematology92(1)11-8(2013 Jan)
Neuwirtova R,Fuchs O,Holicka M,Vostry M,Kostecka A,Hajkova H,Jonasova A,Cermak J,Cmejla R,Pospisilova D,Belickova M,Siskova M,Hochova I,Vondrakova J,Sponerova D,Kadlckova E,Novakova L,Brezinova J,Michalova K

Kr?ppel-like Factor 4 activates HBG gene expression in primary erythroid cells.
British journal of haematology154(2)248-59(2011 Jul)
Kalra IS,Alam MM,Choudhary PK,Pace BS

Mutation in erythroid specific transcription factor KLF1 causes Hereditary Spherocytosis in the Nan hemolytic anemia mouse model.
Genomics96(5)303-7(2010 Nov)
Heruth DP,Hawkins T,Logsdon DP,Gibson MI,Sokolovsky IV,Nsumu NN,Major SL,Fegley B,Woods GM,Lewing KB,Neville KA,Cornetta K,Peterson KR,White RA

A dominant mutation in the gene encoding the erythroid transcription factor KLF1 causes a congenital dyserythropoietic anemia.
American journal of human genetics87(5)721-7(2010 Nov)
Arnaud L,Saison C,Helias V,Lucien N,Steschenko D,Giarratana MC,Prehu C,Foliguet B,Montout L,de Brevern AG,Francina A,Ripoche P,Fenneteau O,Da Costa L,Peyrard T,Coghlan G,Illum N,Birgens H,Tamary H,Iolascon A,Delaunay J,Tchernia G,Cartron JP

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Klf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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