Smurf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smurf2
Entrez Gene ID	303614
Full Name	SMAD specific E3 ubiquitin protein ligase 2
Synonyms	RGD1310067
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase SMURF2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107061.1	NP_001100531.1	E3 ubiquitin-protein ligase SMURF2
XM_017597342.1	XP_017452831.1	E3 ubiquitin-protein ligase SMURF2 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	lack	NP_523779.1
	SMURF2	XP_001109913.2
Bos taurus (cattle)	SMURF2	XP_002696169.3
Gallus gallus (chicken)	SMURF2	XP_425380.2
Homo sapiens (human)	SMURF2	NP_073576.1
Danio rerio (zebrafish)	smurf2	NP_001107898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011242	XP_309403.3
Pan troglodytes (chimpanzee)	SMURF2	XP_511577.3
Canis lupus familiaris (dog)	SMURF2	XP_537589.4
Mus musculus (house mouse)	Smurf2	NP_079757.2
Rattus norvegicus (Norway rat)	Smurf2	NP_001100531.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular mechanism of smurf2 in regulating the expression of SnoN in diabetic nephropathy.
Xu Z, Diao Z, Liu R, Liu W
Molecular medicine reports15(5)2560-2566(2017 May)

Smad ubiquitination regulatory factor-2 controls gap junction intercellular communication by modulating endocytosis and degradation of connexin43.
Fykerud TA, Kjenseth A, Schink KO, Sirnes S, Bruun J, Omori Y, Brech A, Rivedal E, Leithe E
Journal of cell science125(Pt 17)3966-76(2012 Sep)

Overexpression of Smad ubiquitin regulatory factor 2 suppresses transforming growth factor-? mediated liver fibrosis.
Cai Y, Zhou CH, Fu D, Shen XZ
Journal of digestive diseases13(6)327-34(2012 Jun)

Effects of Arkadia on airway remodeling through enhancing TGF-beta signaling in allergic rats.
Li XZ, Feng JT, Hu CP, Chen ZQ, Gu QH, Nie HP
Laboratory investigation; a journal of technical methods and pathology90(7)997-1003(2010 Jul)

Smurf2 participates in human trophoblast cell invasion by inhibiting TGF-beta type I receptor.
Yang Q, Chen SP, Zhang XP, Wang H, Zhu C, Lin HY
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(6)605-12(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data identify Smurf2 as a novel regulator of connexin43 gap junctions.
Title: Smad ubiquitination regulatory factor-2 controls gap junction intercellular communication by modulating endocytosis and degradation of connexin43.

Smurf2 interacts with Smad7 to suppress TGF-beta-mediated liver fibrosis through the ubiquitin-dependent degradation of TbetaRI during the early period of liver fibrosis.
Title: Overexpression of Smad ubiquitin regulatory factor 2 suppresses transforming growth factor-? mediated liver fibrosis.

Smurf2 siRNA did not influence the effects of TGF-beta(1) on the expression of Smad7/SnoN/Ski in airway remodeling.
Title: Effects of Arkadia on airway remodeling through enhancing TGF-beta signaling in allergic rats.

Inhibition of PI3K or mTOR reduced the level of Rap1B, which acts downstream of Rheb and mTOR. The ubiquitin E3 ligase Smurf2 mediates the restriction of Rap1B by initiating its degradation.
Title: Rheb and mTOR regulate neuronal polarity through Rap1B.

There may be a decrease in expression of anabolic factors through cross talk of signaling between TNF-alpha and TGF-beta pathway in pathogenesis of disc degeneration.
Title: Cross talk between Smad transcription factors and TNF-alpha in intervertebral disc degeneration.

The following Smurf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02807	NM_001107061.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa02807	XM_017597342.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smurf2 ( NM_001107061.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100531.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.

Price & Availability↑

CloneID	ORa02807
Clone ID Related Accession (Same CDS sequence)	NM_001107061.1 , XM_017597342.1
Accession Version	NM_001107061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 4, 2007 this sequence version replaced XM_221048.4, XM_001081579.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGACTCCCTG ATCCGTTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAATG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGG AAGTCTGATT CCGTCACGAT CAGCGTGTGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAATCGCCT CAAAGACACT GGCTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA ATCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGCAGTCGTT TGTTTGATAA TGATTTACCA 
GACGGTTGGG AAGAAAGGCG AACTGCCTCT GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACAAGA CCGGCGTCAG AATATTCTAG TCCTGGCAGG 
CCTCTTAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG ACTAGCAGAG AGGAGAGTCC GGTCTCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CCGAAGGCTA TGAACAGAGG 
ACAACACAAC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGCGTGAG CACGTGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTCTGCCT 
CCTGGGTGGG AGATCCGCAA TACCGCGACA GGCAGAGTTT ATTTCGTGGA CCATAACAAC 
AGAACAACAC AGTTTACAGA TCCTCGTCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGT CGTTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG GTACAAGCGA GACTTGGTTC AAAAACTAAA GATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GCATTGAGGT GTCAAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAAGTC ATGAAAATGA GACCAAAGGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG AGGAGAAGAA GGCCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCGCA TGAAATGTTG AATCCTTACT ACGGTCTCTT CCAGTATTCA 
AGAGATGATA TCTACACGTT ACAGATCAAT CCCGATTCTG CAGTTAATCC GGAGCATTTG 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GATGGTGGTT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAC 
GACATGGAGC TAGTTGATCC AGACCTTCAT AATAGTTTGG TGTGGATACT TGAAAATGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAACATA ACGCATATGG TGAAATTATT 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATTCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTACGTAAA CTGGAGATTC CTCCGAGGCA TTGAAGCTCA GTTCCTGGCA 
CTACAGAAGG GATTTAATGA AGTCATTCCA CAGCATCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATCTG TGGGCTTGGC AAAATTGACG TGAGCGACTG GAAGGCCAAC 
ACCCGGTTAA AGCACTGCAC CCCAGACAGC AACGTCGTCA AGTGGTTTTG GAAAGCTGTG 
GAGCTTTTTG ATGAGGAGAG ACGAGCACGG CTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTCCAA GGTGCCGCGG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACCTG CCAAAAGCCC ACACTTGTTT CAATCGAATA 
GACATTCCGC CCTATGAGAG CTATGAGAAG CTCTACGAAA AGCTGCTAAC AGCCATCGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100531.1
CDS	107..2353
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKANTRLKHCTPDSNVVKWFWKAVELFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_001107061.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107061.1

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGACTCCCTG ATCCGTTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAATG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGG AAGTCTGATT CCGTCACGAT CAGCGTGTGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAATCGCCT CAAAGACACT GGCTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA ATCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGCAGTCGTT TGTTTGATAA TGATTTACCA 
GACGGTTGGG AAGAAAGGCG AACTGCCTCT GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACAAGA CCGGCGTCAG AATATTCTAG TCCTGGCAGG 
CCTCTTAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG ACTAGCAGAG AGGAGAGTCC GGTCTCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CCGAAGGCTA TGAACAGAGG 
ACAACACAAC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGCGTGAG CACGTGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTCTGCCT 
CCTGGGTGGG AGATCCGCAA TACCGCGACA GGCAGAGTTT ATTTCGTGGA CCATAACAAC 
AGAACAACAC AGTTTACAGA TCCTCGTCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGT CGTTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG GTACAAGCGA GACTTGGTTC AAAAACTAAA GATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GCATTGAGGT GTCAAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAAGTC ATGAAAATGA GACCAAAGGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG AGGAGAAGAA GGCCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCGCA TGAAATGTTG AATCCTTACT ACGGTCTCTT CCAGTATTCA 
AGAGATGATA TCTACACGTT ACAGATCAAT CCCGATTCTG CAGTTAATCC GGAGCATTTG 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GATGGTGGTT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAC 
GACATGGAGC TAGTTGATCC AGACCTTCAT AATAGTTTGG TGTGGATACT TGAAAATGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAACATA ACGCATATGG TGAAATTATT 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATTCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTACGTAAA CTGGAGATTC CTCCGAGGCA TTGAAGCTCA GTTCCTGGCA 
CTACAGAAGG GATTTAATGA AGTCATTCCA CAGCATCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATCTG TGGGCTTGGC AAAATTGACG TGAGCGACTG GAAGGCCAAC 
ACCCGGTTAA AGCACTGCAC CCCAGACAGC AACGTCGTCA AGTGGTTTTG GAAAGCTGTG 
GAGCTTTTTG ATGAGGAGAG ACGAGCACGG CTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTCCAA GGTGCCGCGG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACCTG CCAAAAGCCC ACACTTGTTT CAATCGAATA 
GACATTCCGC CCTATGAGAG CTATGAGAAG CTCTACGAAA AGCTGCTAAC AGCCATCGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( XM_017597342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452831.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02807
Clone ID Related Accession (Same CDS sequence)	NM_001107061.1 , XM_017597342.1
Accession Version	XM_017597342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) and transcript sequence (NM_001107061.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 90 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGACTCCCTG ATCCGTTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAATG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGG AAGTCTGATT CCGTCACGAT CAGCGTGTGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAATCGCCT CAAAGACACT GGCTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA ATCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGCAGTCGTT TGTTTGATAA TGATTTACCA 
GACGGTTGGG AAGAAAGGCG AACTGCCTCT GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACAAGA CCGGCGTCAG AATATTCTAG TCCTGGCAGG 
CCTCTTAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG ACTAGCAGAG AGGAGAGTCC GGTCTCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CCGAAGGCTA TGAACAGAGG 
ACAACACAAC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGCGTGAG CACGTGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTCTGCCT 
CCTGGGTGGG AGATCCGCAA TACCGCGACA GGCAGAGTTT ATTTCGTGGA CCATAACAAC 
AGAACAACAC AGTTTACAGA TCCTCGTCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGT CGTTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG GTACAAGCGA GACTTGGTTC AAAAACTAAA GATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GCATTGAGGT GTCAAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAAGTC ATGAAAATGA GACCAAAGGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG AGGAGAAGAA GGCCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCGCA TGAAATGTTG AATCCTTACT ACGGTCTCTT CCAGTATTCA 
AGAGATGATA TCTACACGTT ACAGATCAAT CCCGATTCTG CAGTTAATCC GGAGCATTTG 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GATGGTGGTT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAC 
GACATGGAGC TAGTTGATCC AGACCTTCAT AATAGTTTGG TGTGGATACT TGAAAATGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAACATA ACGCATATGG TGAAATTATT 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATTCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTACGTAAA CTGGAGATTC CTCCGAGGCA TTGAAGCTCA GTTCCTGGCA 
CTACAGAAGG GATTTAATGA AGTCATTCCA CAGCATCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATCTG TGGGCTTGGC AAAATTGACG TGAGCGACTG GAAGGCCAAC 
ACCCGGTTAA AGCACTGCAC CCCAGACAGC AACGTCGTCA AGTGGTTTTG GAAAGCTGTG 
GAGCTTTTTG ATGAGGAGAG ACGAGCACGG CTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTCCAA GGTGCCGCGG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACCTG CCAAAAGCCC ACACTTGTTT CAATCGAATA 
GACATTCCGC CCTATGAGAG CTATGAGAAG CTCTACGAAA AGCTGCTAAC AGCCATCGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452831.1
CDS	102..2348
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKANTRLKHCTPDSNVVKWFWKAVELFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_017597342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597342.1

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGACTCCCTG ATCCGTTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAATG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGG AAGTCTGATT CCGTCACGAT CAGCGTGTGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAATCGCCT CAAAGACACT GGCTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA ATCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGCAGTCGTT TGTTTGATAA TGATTTACCA 
GACGGTTGGG AAGAAAGGCG AACTGCCTCT GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACAAGA CCGGCGTCAG AATATTCTAG TCCTGGCAGG 
CCTCTTAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG ACTAGCAGAG AGGAGAGTCC GGTCTCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CCGAAGGCTA TGAACAGAGG 
ACAACACAAC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGCGTGAG CACGTGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTCTGCCT 
CCTGGGTGGG AGATCCGCAA TACCGCGACA GGCAGAGTTT ATTTCGTGGA CCATAACAAC 
AGAACAACAC AGTTTACAGA TCCTCGTCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGT CGTTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG GTACAAGCGA GACTTGGTTC AAAAACTAAA GATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GCATTGAGGT GTCAAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAAGTC ATGAAAATGA GACCAAAGGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG AGGAGAAGAA GGCCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCGCA TGAAATGTTG AATCCTTACT ACGGTCTCTT CCAGTATTCA 
AGAGATGATA TCTACACGTT ACAGATCAAT CCCGATTCTG CAGTTAATCC GGAGCATTTG 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GATGGTGGTT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAC 
GACATGGAGC TAGTTGATCC AGACCTTCAT AATAGTTTGG TGTGGATACT TGAAAATGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAACATA ACGCATATGG TGAAATTATT 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATTCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTACGTAAA CTGGAGATTC CTCCGAGGCA TTGAAGCTCA GTTCCTGGCA 
CTACAGAAGG GATTTAATGA AGTCATTCCA CAGCATCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATCTG TGGGCTTGGC AAAATTGACG TGAGCGACTG GAAGGCCAAC 
ACCCGGTTAA AGCACTGCAC CCCAGACAGC AACGTCGTCA AGTGGTTTTG GAAAGCTGTG 
GAGCTTTTTG ATGAGGAGAG ACGAGCACGG CTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTCCAA GGTGCCGCGG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACCTG CCAAAAGCCC ACACTTGTTT CAATCGAATA 
GACATTCCGC CCTATGAGAG CTATGAGAAG CTCTACGAAA AGCTGCTAAC AGCCATCGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular mechanism of smurf2 in regulating the expression of SnoN in diabetic nephropathy.
Molecular medicine reports15(5)2560-2566(2017 May)
Xu Z,Diao Z,Liu R,Liu W

Smad ubiquitination regulatory factor-2 controls gap junction intercellular communication by modulating endocytosis and degradation of connexin43.
Journal of cell science125(Pt 17)3966-76(2012 Sep)
Fykerud TA,Kjenseth A,Schink KO,Sirnes S,Bruun J,Omori Y,Brech A,Rivedal E,Leithe E

Overexpression of Smad ubiquitin regulatory factor 2 suppresses transforming growth factor-? mediated liver fibrosis.
Journal of digestive diseases13(6)327-34(2012 Jun)
Cai Y,Zhou CH,Fu D,Shen XZ

Effects of Arkadia on airway remodeling through enhancing TGF-beta signaling in allergic rats.
Laboratory investigation; a journal of technical methods and pathology90(7)997-1003(2010 Jul)
Li XZ,Feng JT,Hu CP,Chen ZQ,Gu QH,Nie HP

Smurf2 participates in human trophoblast cell invasion by inhibiting TGF-beta type I receptor.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(6)605-12(2009 Jun)
Yang Q,Chen SP,Zhang XP,Wang H,Zhu C,Lin HY

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Smurf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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