Plekhm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plekhm1
Entrez Gene ID	303584
Full Name	pleckstrin homology and RUN domain containing M1
General protein information	Preferred Names pleckstrin homology and RUN domain containing M1 Names pleckstrin homology domain-containing family M member 1 PH domain-containing family M member 1 pleckstrin homology domain containing, family M (with RUN domain) member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247527.2	XP_006247589.1	pleckstrin homology domain-containing family M member 1 isoform X1
XM_008768292.1	XP_008766514.1	pleckstrin homology domain-containing family M member 1 isoform X2
NM_001009677.1	NP_001009677.1	pleckstrin homology domain-containing family M member 1

Pathways from BioSystems

Homologies

	PLEKHM1	XP_002800605.1
Gallus gallus (chicken)	PLEKHM1	NP_001026095.1
Danio rerio (zebrafish)	plekhm1	NP_001082872.1
Pan troglodytes (chimpanzee)	PLEKHM1	XP_001140671.1
Mus musculus (house mouse)	Plekhm1	NP_898855.1
Bos taurus (cattle)	PLEKHM1	XP_002696128.2
Rattus norvegicus (Norway rat)	Plekhm1	NP_001009677.1
Homo sapiens (human)	PLEKHM1	NP_055613.1
Xenopus tropicalis (tropical clawed frog)	plekhm1	XP_002933223.1

Gene Ontology
Bibliography

Related articles in PubMed

PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis.
Fujiwara T, Ye S, Castro-Gomes T, Winchell CG, Andrews NW, Voth DE, Varughese KI, Mackintosh SG, Feng Y, Pavlos N, Nakamura T, Manolagas SC, Zhao H
JCI insight1(17)e86330(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plekhm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plekhm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa46038	XM_008768292.1 Latest version!	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00799	NM_001009677.1 Latest version!	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$776.30 $1109.00
ORa33818	XM_006247527.2 Latest version!	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plekhm1 ( XM_008768292.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766514.1 Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa46038
Clone ID Related Accession (Same CDS sequence)	XM_008768292.1
Accession Version	XM_008768292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pleckstrin homology domain-containing family M member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGA CCCTCGGGCT GCCATCCAGA CACATTATCT CAGACTTGGA GCACCTGGTC 
TTCATCAATA CAGACGTAGG CCGCTGCCGG GCCTGGCTGC GGTTGGCCCT CAATGATGGG 
CTGATGGAGT GCTACCTGAA GTTGCTCCTG CAGGAACCTG CACGGTTGTG CGAGTACTAT 
CAGCCCACGG CCCTGCTTCG AGATGCTGAG GAAGCCGAGT TCCTCCTGAG CTTCCTCCAA 
GGACTAACAT CCTTGTCTTT TGAACTCTCC TACAAGTCAG CCATCTTAAA TGAATGGACA 
CTCACCCCAC TGTCTCTCTC TGGGCTTTGC CCCCTCTCTG AACTGGACCC TCTCACTGTC 
TCTGGTGCAG AACTGCAACG GAAGGAGTCT CTGGATTCCA TTTCCCATTC CTCAGGCTCT 
GAGGACATCG AAGTCCAACA CTCAGGCCAT AAGATCCGCA GGGACAGGAA GCTCACCGCC 
TCTTCCCTCA GCCTGGACAC AGCCAGCTCA TCCCAGCTAT CCTGCAGCCT AAATTCTGAC 
AGCTGCCTGC TCCAAGAGAA TGGTCCCAAG AGTCCAGACC ACTCTGAGGA ACCCATGTCC 
TACGACTCAG ATCTGGGCAC AGCAAATGCT GATGACTCTG ACAGGTCTCT GCAAGAGGTG 
TTGTCGGAAT TCAGCAAAGC CCAGGTAAAT TCTGCACCAA GCAGCGGACC AAGCCAAGAG 
TCAGACACCC CCATGTTCCA GACCCCCCTC TCCCTCCACA GCCTAGCTAA CAGCACACAC 
CTGCTCTTTG AAGGATCTGA AGAGCCCTTT CCTGCCCATA CATCCTCTGG GACTTCAAGT 
GGCCACAAGC ATCAGCCCCA GGAGAGTCCA GACATGCAGC CGCTAGGCAC TGCCCAAGCT 
GGCCCTGCTG GGAGCACTTC AGACCAGCAG CCATCTAGTC CCGTGGCCGG AGCAGCTGAC 
CAAGGAAGTG AGCCTTGGAA GGCCCTGGAA TATGGCAGAG TTGGACCGAA GCTTGTGGTT 
TCCTCTCCTA CGAGTCCGAA AGGCAAGAGC TGGATTTCTG AAGATGACTT CTGCCGACCT 
CCCAAGGAGC ATGCTCTGAA GAACACTTCA GATCTTTGCA TATCTCCTCT GCAAGGGACT 
CCAGAGTTGA GGACTGCTCT CCACGGGCCG TTTTCTCAAG GACCAAGAAA GAGCTGCTCC 
CTGGGGGCCT TAGACAAAGC GTGTGTGTCC TCGCTGGACT ACAGAAATGC CCAGACAGCT 
CCATCTCCAG CGGTGACACA AGACCATAAA AACTTCTGTG TGGTGCATCG AAGGCAGATG 
GGGCTCTCTA ACCCATTCCG GGGCCTCATG AAGCTGGGCA CAGTAGCCCG GCGAGGAGCC 
ATGGGCATCT GGAAGGAATT CTTTTGTGAG CTGTCCCCAC TGGAGCTCCG CCTCTACCTG 
AGTGACGAGG AGCGCACCTG TGTGGAGAGC TGCTCCCTGC TACGGTGTGA GGCCGTGGGA 
CCAGCACACA GCGATGGACG TTTTGAGTTG GTCTTCTCCG GCAAGAAGCT GGCTCTCCGT 
GCCTCCTCCC AGGACGAGGC TGAGGACTGG CTTGACCGTG TGCGGGAGGC TCTGCAGAAG 
GTGCGCCCTC AGCAGGAGGA GGAGTGGGTG AATATCCAGT ACCCAGACCA GGCTGAGGAT 
TCCCCAGAGG CGCCACCAGA CAACCTTCCC CCCTATTCAG CCCTGCTCCC AGAACATGCA 
GGTGCACAGG GAATACAGCC AAACTGGACA TCTGCCCAGG TTCCAGAGCC AGATGCTATC 
AAAGAGTCTC TTTTGTACCT GTATGCAGAC CGGACCTGGA TTCCCTATAT CTTTTCCCTG 
TCCTTGGAGT CTCTGAAATG TTTCCGTGTG AGAAACAATG AGAAGATGCT AAGTGATAGC 
CATGGAGTGG AGACCATCCG GGACATCCTG CCAGACACCA GCCTTGGGGG CCCAGCCTTC 
TTCAAAATCA TCACGGCCAA GGCTGTCCTC AAACTGCAGG CCAAGAACAC TGAGGAGGCC 
GCCCACTGGA GAGACCTAGT CAGGAAAGTG CTGGCGTCTT ACTTAGAGTC AGTTCAGGAG 
GCTGTGACAC TTGCTGGGAG TCTGGATGAA AACTGTCAGG AGGTCCTGAA GTTTGCCACC 
AGGGAGAATG GCTTCCTGCT ACAGTACCTT GTGGCCATCC CGACCGAGAA GGGCCTCGAC 
TCCCAGGGCT GCTTCTGTGC AGGTTGCTCC CGGCAGATCG GCTTCTCCTT TGTACGACCT 
AAGCTCTGTG CCTTCTCTGG CCTATATTAC TGTGACTTCT GCCACCAAGA CGATGCCTCG 
GTGATTCCAG CCAGAATCAT TCATAACTGG GACCTCACAA AGCGCCCGGT CTGCCGGCAG 
GCCCTGAAGT TCCTGGCCCA GATCCGGGCC CAGCCCCTCA TCAACCTGCA GCTGGTGAAC 
GCCTCTCTGT ATGAGCATGT AGAGCGCATG CACCTCATTG GCAGGAGCCG GGAGCAGCTG 
AAACTTCTGG GGGACTACCT GGGCCTGTGC CGAAGTGGTG CTCTGAAGGA GTTGAGCAAA 
AGGCTAAGCC ACAGGAATTA CCTCTTGGAG TCTCCTCACA AATTCAGTGT TGCTGACCTC 
CAGCAGATTG CAGAGGGGGT GTATGAAGGA TTCCTCAAAG CCCTGATTGA ATTCGCCTCC 
CAGCATGTCT ACCACTGTGA CCTGTGCACC CAGCGAGGCT TCATCTGCCA GATCTGCCAC 
CACCAGGACA TCATCTTTCC CTTTGAGTTT GACACCACAG TCAGGTGTGC TGAATGCAGA 
ACTGTCTTCC ATCAGAGCTG CCAGGCTGTG GTGAGGAAGG GCTGTCCCCG CTGTGCCCGC 
CGGCGCAAGT ACCAGGAACA GAACACTGTC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766514.1
CDS	182..3097
Translation	MAWTLGLPSRHIISDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTVSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRDRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGTANADDSDRSLQEVLSEFSKAQVNSAPSSGPSQESDTPMFQTPLSLHSLANSTHLLFEGSEEPFPAHTSSGTSSGHKHQPQESPDMQPLGTAQAGPAGSTSDQQPSSPVAGAADQGSEPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPKEHALKNTSDLCISPLQGTPELRTALHGPFSQGPRKSCSLGALDKACVSSLDYRNAQTAPSPAVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLELRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEEEWVNIQYPDQAEDSPEAPPDNLPPYSALLPEHAGAQGIQPNWTSAQVPEPDAIKESLLYLYADRTWIPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEAAHWRDLVRKVLASYLESVQEAVTLAGSLDENCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELSKRLSHRNYLLESPHKFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNTVS

Target ORF information:

RefSeq Version	XM_008768292.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768292.1

ORF Insert Sequence:

ATGGCCTGGA CCCTCGGGCT GCCATCCAGA CACATTATCT CAGACTTGGA GCACCTGGTC 
TTCATCAATA CAGACGTAGG CCGCTGCCGG GCCTGGCTGC GGTTGGCCCT CAATGATGGG 
CTGATGGAGT GCTACCTGAA GTTGCTCCTG CAGGAACCTG CACGGTTGTG CGAGTACTAT 
CAGCCCACGG CCCTGCTTCG AGATGCTGAG GAAGCCGAGT TCCTCCTGAG CTTCCTCCAA 
GGACTAACAT CCTTGTCTTT TGAACTCTCC TACAAGTCAG CCATCTTAAA TGAATGGACA 
CTCACCCCAC TGTCTCTCTC TGGGCTTTGC CCCCTCTCTG AACTGGACCC TCTCACTGTC 
TCTGGTGCAG AACTGCAACG GAAGGAGTCT CTGGATTCCA TTTCCCATTC CTCAGGCTCT 
GAGGACATCG AAGTCCAACA CTCAGGCCAT AAGATCCGCA GGGACAGGAA GCTCACCGCC 
TCTTCCCTCA GCCTGGACAC AGCCAGCTCA TCCCAGCTAT CCTGCAGCCT AAATTCTGAC 
AGCTGCCTGC TCCAAGAGAA TGGTCCCAAG AGTCCAGACC ACTCTGAGGA ACCCATGTCC 
TACGACTCAG ATCTGGGCAC AGCAAATGCT GATGACTCTG ACAGGTCTCT GCAAGAGGTG 
TTGTCGGAAT TCAGCAAAGC CCAGGTAAAT TCTGCACCAA GCAGCGGACC AAGCCAAGAG 
TCAGACACCC CCATGTTCCA GACCCCCCTC TCCCTCCACA GCCTAGCTAA CAGCACACAC 
CTGCTCTTTG AAGGATCTGA AGAGCCCTTT CCTGCCCATA CATCCTCTGG GACTTCAAGT 
GGCCACAAGC ATCAGCCCCA GGAGAGTCCA GACATGCAGC CGCTAGGCAC TGCCCAAGCT 
GGCCCTGCTG GGAGCACTTC AGACCAGCAG CCATCTAGTC CCGTGGCCGG AGCAGCTGAC 
CAAGGAAGTG AGCCTTGGAA GGCCCTGGAA TATGGCAGAG TTGGACCGAA GCTTGTGGTT 
TCCTCTCCTA CGAGTCCGAA AGGCAAGAGC TGGATTTCTG AAGATGACTT CTGCCGACCT 
CCCAAGGAGC ATGCTCTGAA GAACACTTCA GATCTTTGCA TATCTCCTCT GCAAGGGACT 
CCAGAGTTGA GGACTGCTCT CCACGGGCCG TTTTCTCAAG GACCAAGAAA GAGCTGCTCC 
CTGGGGGCCT TAGACAAAGC GTGTGTGTCC TCGCTGGACT ACAGAAATGC CCAGACAGCT 
CCATCTCCAG CGGTGACACA AGACCATAAA AACTTCTGTG TGGTGCATCG AAGGCAGATG 
GGGCTCTCTA ACCCATTCCG GGGCCTCATG AAGCTGGGCA CAGTAGCCCG GCGAGGAGCC 
ATGGGCATCT GGAAGGAATT CTTTTGTGAG CTGTCCCCAC TGGAGCTCCG CCTCTACCTG 
AGTGACGAGG AGCGCACCTG TGTGGAGAGC TGCTCCCTGC TACGGTGTGA GGCCGTGGGA 
CCAGCACACA GCGATGGACG TTTTGAGTTG GTCTTCTCCG GCAAGAAGCT GGCTCTCCGT 
GCCTCCTCCC AGGACGAGGC TGAGGACTGG CTTGACCGTG TGCGGGAGGC TCTGCAGAAG 
GTGCGCCCTC AGCAGGAGGA GGAGTGGGTG AATATCCAGT ACCCAGACCA GGCTGAGGAT 
TCCCCAGAGG CGCCACCAGA CAACCTTCCC CCCTATTCAG CCCTGCTCCC AGAACATGCA 
GGTGCACAGG GAATACAGCC AAACTGGACA TCTGCCCAGG TTCCAGAGCC AGATGCTATC 
AAAGAGTCTC TTTTGTACCT GTATGCAGAC CGGACCTGGA TTCCCTATAT CTTTTCCCTG 
TCCTTGGAGT CTCTGAAATG TTTCCGTGTG AGAAACAATG AGAAGATGCT AAGTGATAGC 
CATGGAGTGG AGACCATCCG GGACATCCTG CCAGACACCA GCCTTGGGGG CCCAGCCTTC 
TTCAAAATCA TCACGGCCAA GGCTGTCCTC AAACTGCAGG CCAAGAACAC TGAGGAGGCC 
GCCCACTGGA GAGACCTAGT CAGGAAAGTG CTGGCGTCTT ACTTAGAGTC AGTTCAGGAG 
GCTGTGACAC TTGCTGGGAG TCTGGATGAA AACTGTCAGG AGGTCCTGAA GTTTGCCACC 
AGGGAGAATG GCTTCCTGCT ACAGTACCTT GTGGCCATCC CGACCGAGAA GGGCCTCGAC 
TCCCAGGGCT GCTTCTGTGC AGGTTGCTCC CGGCAGATCG GCTTCTCCTT TGTACGACCT 
AAGCTCTGTG CCTTCTCTGG CCTATATTAC TGTGACTTCT GCCACCAAGA CGATGCCTCG 
GTGATTCCAG CCAGAATCAT TCATAACTGG GACCTCACAA AGCGCCCGGT CTGCCGGCAG 
GCCCTGAAGT TCCTGGCCCA GATCCGGGCC CAGCCCCTCA TCAACCTGCA GCTGGTGAAC 
GCCTCTCTGT ATGAGCATGT AGAGCGCATG CACCTCATTG GCAGGAGCCG GGAGCAGCTG 
AAACTTCTGG GGGACTACCT GGGCCTGTGC CGAAGTGGTG CTCTGAAGGA GTTGAGCAAA 
AGGCTAAGCC ACAGGAATTA CCTCTTGGAG TCTCCTCACA AATTCAGTGT TGCTGACCTC 
CAGCAGATTG CAGAGGGGGT GTATGAAGGA TTCCTCAAAG CCCTGATTGA ATTCGCCTCC 
CAGCATGTCT ACCACTGTGA CCTGTGCACC CAGCGAGGCT TCATCTGCCA GATCTGCCAC 
CACCAGGACA TCATCTTTCC CTTTGAGTTT GACACCACAG TCAGGTGTGC TGAATGCAGA 
ACTGTCTTCC ATCAGAGCTG CCAGGCTGTG GTGAGGAAGG GCTGTCCCCG CTGTGCCCGC 
CGGCGCAAGT ACCAGGAACA GAACACTGTC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plekhm1 ( NM_001009677.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009677.1 Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), mRNA.

Price & Availability↑

CloneID	ORa00799
Clone ID Related Accession (Same CDS sequence)	NM_001009677.1
Accession Version	NM_001009677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Rattus norvegicus(Norway rat)
Product	pleckstrin homology domain-containing family M member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087058.1. On Jan 13, 2005 this sequence version replaced XM_221013.2. ##Evidence-Data-START## Transcript exon combination :: BC087058.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGGGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTAGTGGGAT CTGTGAAGGC CCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCTAA CACCATGTGC AGTGCCCTGG AGGCCGTGTT CATCCATGGC 
TTGCATGCCA AGTACATCCG TGCTGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCATCAG 
AAGCCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CTGTCACCCA CAAACACATT 
ATCTCAGACT TGGAGCACCT GGTCTTCATC AATACAGACG TAGGCCGCTG CCGGGCCTGG 
CTGCGGTTGG CCCTCAATGA TGGGCTGATG GAGTGCTACC TGAAGTTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACGGCCCTGC TTCGAGATGC TGAGGAAGCC 
GAGTTCCTCC TGAGCTTCCT CCAAGGACTA ACATCCTTGT CTTTTGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCACTGTCTC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTGG ACCCTCTCAC TGTCTCTGGT GCAGAACTGC AACGGAAGGA GTCTCTGGAT 
TCCATTTCCC ATTCCTCAGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGGACA GGAAGCTCAC CGCCTCTTCC CTCAGCCTGG ACACAGCCAG CTCATCCCAG 
CTATCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCACTCTG AGGAACCCAT GTCCTACGAC TCAGATCTGG GCACAGCAAA TGCTGATGAC 
TCTGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAGCCA AGAGTCAGAC ACCCCCATGT TCCAGACCCC CCTCTCCCTC 
CACAGCCTAG CTAACAGCAC ACACCTGCTC TTTGAAGGAT CTGAAGAGCC CTTTCCTGCC 
CATACATCCT CTGGGACTTC AAGTGGCCAC AAGCATCAGC CCCAGGAGAG TCCAGACATG 
CAGCCGCTAG GCACTGCCCA AGCTGGCCCT GCTGGGAGCA CTTCAGACCA GCAGCCATCT 
AGTCCCGTGG CCGGAGCAGC TGACCAAGGA AGTGAGCCTT GGAAGGCCCT GGAATATGGC 
AGAGTTGGAC CGAAGCTTGT GGTTTCCTCT CCTACGAGTC CGAAAGGCAA GAGCTGGATT 
TCTGAAGATG ACTTCTGCCG ACCTCCCAAG GAGCATGCTC TGAAGAACAC TTCAGATCTT 
TGCATATCTC CTCTGCAAGG GACTCCAGAG TTGAGGACTG CTCTCCACGG GCCGTTTTCT 
CAAGGACCAA GAAAGAGCTG CTCCCTGGGG GCCTTAGACA AAGCGTGTGT GTCCTCGCTG 
GACTACAGAA ATGCCCAGAC AGCTCCATCT CCAGCGGTGA CACAAGACCA TAAAAACTTC 
TGTGTGGTGC ATCGAAGGCA GATGGGGCTC TCTAACCCAT TCCGGGGCCT CATGAAGCTG 
GGCACAGTAG CCCGGCGAGG AGCCATGGGC ATCTGGAAGG AATTCTTTTG TGAGCTGTCC 
CCACTGGAGC TCCGCCTCTA CCTGAGTGAC GAGGAGCGCA CCTGTGTGGA GAGCTGCTCC 
CTGCTACGGT GTGAGGCCGT GGGACCAGCA CACAGCGATG GACGTTTTGA GTTGGTCTTC 
TCCGGCAAGA AGCTGGCTCT CCGTGCCTCC TCCCAGGACG AGGCTGAGGA CTGGCTTGAC 
CGTGTGCGGG AGGCTCTGCA GAAGGTGCGC CCTCAGCAGG AGGAGGAGTG GGTGAATATC 
CAGTACCCAG ACCAGGCTGA GGATTCCCCA GAGGCGCCAC CAGACAACCT TCCCCCCTAT 
TCAGCCCTGC TCCCAGAACA TGCAGGTGCA CAGGGAATAC AGCCAAACTG GACATCTGCC 
CAGGTTCCAG AGCCAGATGC TATCAAAGAG TCTCTTTTGT ACCTGTATGC AGACCGGACC 
TGGATTCCCT ATATCTTTTC CCTGTCCTTG GAGTCTCTGA AATGTTTCCG TGTGAGAAAC 
AATGAGAAGA TGCTAAGTGA TAGCCATGGA GTGGAGACCA TCCGGGACAT CCTGCCAGAC 
ACCAGCCTTG GGGGCCCAGC CTTCTTCAAA ATCATCACGG CCAAGGCTGT CCTCAAACTG 
CAGGCCAAGA ACACTGAGGA GGCCGCCCAC TGGAGAGACC TAGTCAGGAA AGTGCTGGCG 
TCTTACTTAG AGTCAGTTCA GGAGGCTGTG ACACTTGCTG GGAGTCTGGA TGAAAACTGT 
CAGGAGGTCC TGAAGTTTGC CACCAGGGAG AATGGCTTCC TGCTACAGTA CCTTGTGGCC 
ATCCCGACCG AGAAGGGCCT CGACTCCCAG GGCTGCTTCT GTGCAGGTTG CTCCCGGCAG 
ATCGGCTTCT CCTTTGTACG ACCTAAGCTC TGTGCCTTCT CTGGCCTATA TTACTGTGAC 
TTCTGCCACC AAGACGATGC CTCGGTGATT CCAGCCAGAA TCATTCATAA CTGGGACCTC 
ACAAAGCGCC CGGTCTGCCG GCAGGCCCTG AAGTTCCTGG CCCAGATCCG GGCCCAGCCC 
CTCATCAACC TGCAGCTGGT GAACGCCTCT CTGTATGAGC ATGTAGAGCG CATGCACCTC 
ATTGGCAGGA GCCGGGAGCA GCTGAAACTT CTGGGGGACT ACCTGGGCCT GTGCCGAAGT 
GGTGCTCTGA AGGAGTTGAG CAAAAGGCTA AGCCACAGGA ATTACCTCTT GGAGTCTCCT 
CACAAATTCA GTGTTGCTGA CCTCCAGCAG ATTGCAGAGG GGGTGTATGA AGGATTCCTC 
AAAGCCCTGA TTGAATTCGC CTCCCAGCAT GTCTACCACT GTGACCTGTG CACCCAGCGA 
GGCTTCATCT GCCAGATCTG CCACCACCAG GACATCATCT TTCCCTTTGA GTTTGACACC 
ACAGTCAGGT GTGCTGAATG CAGAACTGTC TTCCATCAGA GCTGCCAGGC TGTGGTGAGG 
AAGGGCTGTC CCCGCTGTGC CCGCCGGCGC AAGTACCAGG AACAGAACAC TGTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009677.1
CDS	98..3277
Translation	MLSVENGLDPRAAIQVIKKKLVGSVKALQKQHVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKYIRAEAGGKRKKHTHQKPLPQPVFWPLLKAVTHKHIISDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTVSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRDRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGTANADDSDRSLQEVLSEFSKAQVNSAPSSGPSQESDTPMFQTPLSLHSLANSTHLLFEGSEEPFPAHTSSGTSSGHKHQPQESPDMQPLGTAQAGPAGSTSDQQPSSPVAGAADQGSEPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPKEHALKNTSDLCISPLQGTPELRTALHGPFSQGPRKSCSLGALDKACVSSLDYRNAQTAPSPAVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLELRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEEEWVNIQYPDQAEDSPEAPPDNLPPYSALLPEHAGAQGIQPNWTSAQVPEPDAIKESLLYLYADRTWIPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEAAHWRDLVRKVLASYLESVQEAVTLAGSLDENCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELSKRLSHRNYLLESPHKFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNTVS

Target ORF information:

RefSeq Version	NM_001009677.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009677.1

ORF Insert Sequence:

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGGGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTAGTGGGAT CTGTGAAGGC CCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCTAA CACCATGTGC AGTGCCCTGG AGGCCGTGTT CATCCATGGC 
TTGCATGCCA AGTACATCCG TGCTGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCATCAG 
AAGCCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CTGTCACCCA CAAACACATT 
ATCTCAGACT TGGAGCACCT GGTCTTCATC AATACAGACG TAGGCCGCTG CCGGGCCTGG 
CTGCGGTTGG CCCTCAATGA TGGGCTGATG GAGTGCTACC TGAAGTTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACGGCCCTGC TTCGAGATGC TGAGGAAGCC 
GAGTTCCTCC TGAGCTTCCT CCAAGGACTA ACATCCTTGT CTTTTGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCACTGTCTC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTGG ACCCTCTCAC TGTCTCTGGT GCAGAACTGC AACGGAAGGA GTCTCTGGAT 
TCCATTTCCC ATTCCTCAGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGGACA GGAAGCTCAC CGCCTCTTCC CTCAGCCTGG ACACAGCCAG CTCATCCCAG 
CTATCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCACTCTG AGGAACCCAT GTCCTACGAC TCAGATCTGG GCACAGCAAA TGCTGATGAC 
TCTGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAGCCA AGAGTCAGAC ACCCCCATGT TCCAGACCCC CCTCTCCCTC 
CACAGCCTAG CTAACAGCAC ACACCTGCTC TTTGAAGGAT CTGAAGAGCC CTTTCCTGCC 
CATACATCCT CTGGGACTTC AAGTGGCCAC AAGCATCAGC CCCAGGAGAG TCCAGACATG 
CAGCCGCTAG GCACTGCCCA AGCTGGCCCT GCTGGGAGCA CTTCAGACCA GCAGCCATCT 
AGTCCCGTGG CCGGAGCAGC TGACCAAGGA AGTGAGCCTT GGAAGGCCCT GGAATATGGC 
AGAGTTGGAC CGAAGCTTGT GGTTTCCTCT CCTACGAGTC CGAAAGGCAA GAGCTGGATT 
TCTGAAGATG ACTTCTGCCG ACCTCCCAAG GAGCATGCTC TGAAGAACAC TTCAGATCTT 
TGCATATCTC CTCTGCAAGG GACTCCAGAG TTGAGGACTG CTCTCCACGG GCCGTTTTCT 
CAAGGACCAA GAAAGAGCTG CTCCCTGGGG GCCTTAGACA AAGCGTGTGT GTCCTCGCTG 
GACTACAGAA ATGCCCAGAC AGCTCCATCT CCAGCGGTGA CACAAGACCA TAAAAACTTC 
TGTGTGGTGC ATCGAAGGCA GATGGGGCTC TCTAACCCAT TCCGGGGCCT CATGAAGCTG 
GGCACAGTAG CCCGGCGAGG AGCCATGGGC ATCTGGAAGG AATTCTTTTG TGAGCTGTCC 
CCACTGGAGC TCCGCCTCTA CCTGAGTGAC GAGGAGCGCA CCTGTGTGGA GAGCTGCTCC 
CTGCTACGGT GTGAGGCCGT GGGACCAGCA CACAGCGATG GACGTTTTGA GTTGGTCTTC 
TCCGGCAAGA AGCTGGCTCT CCGTGCCTCC TCCCAGGACG AGGCTGAGGA CTGGCTTGAC 
CGTGTGCGGG AGGCTCTGCA GAAGGTGCGC CCTCAGCAGG AGGAGGAGTG GGTGAATATC 
CAGTACCCAG ACCAGGCTGA GGATTCCCCA GAGGCGCCAC CAGACAACCT TCCCCCCTAT 
TCAGCCCTGC TCCCAGAACA TGCAGGTGCA CAGGGAATAC AGCCAAACTG GACATCTGCC 
CAGGTTCCAG AGCCAGATGC TATCAAAGAG TCTCTTTTGT ACCTGTATGC AGACCGGACC 
TGGATTCCCT ATATCTTTTC CCTGTCCTTG GAGTCTCTGA AATGTTTCCG TGTGAGAAAC 
AATGAGAAGA TGCTAAGTGA TAGCCATGGA GTGGAGACCA TCCGGGACAT CCTGCCAGAC 
ACCAGCCTTG GGGGCCCAGC CTTCTTCAAA ATCATCACGG CCAAGGCTGT CCTCAAACTG 
CAGGCCAAGA ACACTGAGGA GGCCGCCCAC TGGAGAGACC TAGTCAGGAA AGTGCTGGCG 
TCTTACTTAG AGTCAGTTCA GGAGGCTGTG ACACTTGCTG GGAGTCTGGA TGAAAACTGT 
CAGGAGGTCC TGAAGTTTGC CACCAGGGAG AATGGCTTCC TGCTACAGTA CCTTGTGGCC 
ATCCCGACCG AGAAGGGCCT CGACTCCCAG GGCTGCTTCT GTGCAGGTTG CTCCCGGCAG 
ATCGGCTTCT CCTTTGTACG ACCTAAGCTC TGTGCCTTCT CTGGCCTATA TTACTGTGAC 
TTCTGCCACC AAGACGATGC CTCGGTGATT CCAGCCAGAA TCATTCATAA CTGGGACCTC 
ACAAAGCGCC CGGTCTGCCG GCAGGCCCTG AAGTTCCTGG CCCAGATCCG GGCCCAGCCC 
CTCATCAACC TGCAGCTGGT GAACGCCTCT CTGTATGAGC ATGTAGAGCG CATGCACCTC 
ATTGGCAGGA GCCGGGAGCA GCTGAAACTT CTGGGGGACT ACCTGGGCCT GTGCCGAAGT 
GGTGCTCTGA AGGAGTTGAG CAAAAGGCTA AGCCACAGGA ATTACCTCTT GGAGTCTCCT 
CACAAATTCA GTGTTGCTGA CCTCCAGCAG ATTGCAGAGG GGGTGTATGA AGGATTCCTC 
AAAGCCCTGA TTGAATTCGC CTCCCAGCAT GTCTACCACT GTGACCTGTG CACCCAGCGA 
GGCTTCATCT GCCAGATCTG CCACCACCAG GACATCATCT TTCCCTTTGA GTTTGACACC 
ACAGTCAGGT GTGCTGAATG CAGAACTGTC TTCCATCAGA GCTGCCAGGC TGTGGTGAGG 
AAGGGCTGTC CCCGCTGTGC CCGCCGGCGC AAGTACCAGG AACAGAACAC TGTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plekhm1 ( XM_006247527.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247589.1 Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X1, mRNA.

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CloneID	ORa33818
Clone ID Related Accession (Same CDS sequence)	XM_006247527.2
Accession Version	XM_006247527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pleckstrin homology domain-containing family M member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGTG CCCTGGAGGC CGTGTTCATC CATGGCTTGC ATGCCAAGTA CATCCGTGCT 
GAGGCTGGCG GCAAAAGGAA GAAGCACACT CATCAGAAGC CTCTGCCTCA GCCTGTCTTC 
TGGCCCCTTC TGAAAGCTGT CACCCACAAA CACATTATCT CAGACTTGGA GCACCTGGTC 
TTCATCAATA CAGACGTAGG CCGCTGCCGG GCCTGGCTGC GGTTGGCCCT CAATGATGGG 
CTGATGGAGT GCTACCTGAA GTTGCTCCTG CAGGAACCTG CACGGTTGTG CGAGTACTAT 
CAGCCCACGG CCCTGCTTCG AGATGCTGAG GAAGCCGAGT TCCTCCTGAG CTTCCTCCAA 
GGACTAACAT CCTTGTCTTT TGAACTCTCC TACAAGTCAG CCATCTTAAA TGAATGGACA 
CTCACCCCAC TGTCTCTCTC TGGGCTTTGC CCCCTCTCTG AACTGGACCC TCTCACTGTC 
TCTGGTGCAG AACTGCAACG GAAGGAGTCT CTGGATTCCA TTTCCCATTC CTCAGGCTCT 
GAGGACATCG AAGTCCAACA CTCAGGCCAT AAGATCCGCA GGGACAGGAA GCTCACCGCC 
TCTTCCCTCA GCCTGGACAC AGCCAGCTCA TCCCAGCTAT CCTGCAGCCT AAATTCTGAC 
AGCTGCCTGC TCCAAGAGAA TGGTCCCAAG AGTCCAGACC ACTCTGAGGA ACCCATGTCC 
TACGACTCAG ATCTGGGCAC AGCAAATGCT GATGACTCTG ACAGGTCTCT GCAAGAGGTG 
TTGTCGGAAT TCAGCAAAGC CCAGGTAAAT TCTGCACCAA GCAGCGGACC AAGCCAAGAG 
TCAGACACCC CCATGTTCCA GACCCCCCTC TCCCTCCACA GCCTAGCTAA CAGCACACAC 
CTGCTCTTTG AAGGATCTGA AGAGCCCTTT CCTGCCCATA CATCCTCTGG GACTTCAAGT 
GGCCACAAGC ATCAGCCCCA GGAGAGTCCA GACATGCAGC CGCTAGGCAC TGCCCAAGCT 
GGCCCTGCTG GGAGCACTTC AGACCAGCAG CCATCTAGTC CCGTGGCCGG AGCAGCTGAC 
CAAGGAAGTG AGCCTTGGAA GGCCCTGGAA TATGGCAGAG TTGGACCGAA GCTTGTGGTT 
TCCTCTCCTA CGAGTCCGAA AGGCAAGAGC TGGATTTCTG AAGATGACTT CTGCCGACCT 
CCCAAGGAGC ATGCTCTGAA GAACACTTCA GATCTTTGCA TATCTCCTCT GCAAGGGACT 
CCAGAGTTGA GGACTGCTCT CCACGGGCCG TTTTCTCAAG GACCAAGAAA GAGCTGCTCC 
CTGGGGGCCT TAGACAAAGC GTGTGTGTCC TCGCTGGACT ACAGAAATGC CCAGACAGCT 
CCATCTCCAG CGGTGACACA AGACCATAAA AACTTCTGTG TGGTGCATCG AAGGCAGATG 
GGGCTCTCTA ACCCATTCCG GGGCCTCATG AAGCTGGGCA CAGTAGCCCG GCGAGGAGCC 
ATGGGCATCT GGAAGGAATT CTTTTGTGAG CTGTCCCCAC TGGAGCTCCG CCTCTACCTG 
AGTGACGAGG AGCGCACCTG TGTGGAGAGC TGCTCCCTGC TACGGTGTGA GGCCGTGGGA 
CCAGCACACA GCGATGGACG TTTTGAGTTG GTCTTCTCCG GCAAGAAGCT GGCTCTCCGT 
GCCTCCTCCC AGGACGAGGC TGAGGACTGG CTTGACCGTG TGCGGGAGGC TCTGCAGAAG 
GTGCGCCCTC AGCAGGAGGA GGAGTGGGTG AATATCCAGT ACCCAGACCA GGCTGAGGAT 
TCCCCAGAGG CGCCACCAGA CAACCTTCCC CCCTATTCAG CCCTGCTCCC AGAACATGCA 
GGTGCACAGG GAATACAGCC AAACTGGACA TCTGCCCAGG TTCCAGAGCC AGATGCTATC 
AAAGAGTCTC TTTTGTACCT GTATGCAGAC CGGACCTGGA TTCCCTATAT CTTTTCCCTG 
TCCTTGGAGT CTCTGAAATG TTTCCGTGTG AGAAACAATG AGAAGATGCT AAGTGATAGC 
CATGGAGTGG AGACCATCCG GGACATCCTG CCAGACACCA GCCTTGGGGG CCCAGCCTTC 
TTCAAAATCA TCACGGCCAA GGCTGTCCTC AAACTGCAGG CCAAGAACAC TGAGGAGGCC 
GCCCACTGGA GAGACCTAGT CAGGAAAGTG CTGGCGTCTT ACTTAGAGTC AGTTCAGGAG 
GCTGTGACAC TTGCTGGGAG TCTGGATGAA AACTGTCAGG AGGTCCTGAA GTTTGCCACC 
AGGGAGAATG GCTTCCTGCT ACAGTACCTT GTGGCCATCC CGACCGAGAA GGGCCTCGAC 
TCCCAGGGCT GCTTCTGTGC AGGTTGCTCC CGGCAGATCG GCTTCTCCTT TGTACGACCT 
AAGCTCTGTG CCTTCTCTGG CCTATATTAC TGTGACTTCT GCCACCAAGA CGATGCCTCG 
GTGATTCCAG CCAGAATCAT TCATAACTGG GACCTCACAA AGCGCCCGGT CTGCCGGCAG 
GCCCTGAAGT TCCTGGCCCA GATCCGGGCC CAGCCCCTCA TCAACCTGCA GCTGGTGAAC 
GCCTCTCTGT ATGAGCATGT AGAGCGCATG CACCTCATTG GCAGGAGCCG GGAGCAGCTG 
AAACTTCTGG GGGACTACCT GGGCCTGTGC CGAAGTGGTG CTCTGAAGGA GTTGAGCAAA 
AGGCTAAGCC ACAGGAATTA CCTCTTGGAG TCTCCTCACA AATTCAGTGT TGCTGACCTC 
CAGCAGATTG CAGAGGGGGT GTATGAAGGA TTCCTCAAAG CCCTGATTGA ATTCGCCTCC 
CAGCATGTCT ACCACTGTGA CCTGTGCACC CAGCGAGGCT TCATCTGCCA GATCTGCCAC 
CACCAGGACA TCATCTTTCC CTTTGAGTTT GACACCACAG TCAGGTGTGC TGAATGCAGA 
ACTGTCTTCC ATCAGAGCTG CCAGGCTGTG GTGAGGAAGG GCTGTCCCCG CTGTGCCCGC 
CGGCGCAAGT ACCAGGAACA GAACACTGTC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247589.1
CDS	282..3317
Translation	MCSALEAVFIHGLHAKYIRAEAGGKRKKHTHQKPLPQPVFWPLLKAVTHKHIISDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTVSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRDRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGTANADDSDRSLQEVLSEFSKAQVNSAPSSGPSQESDTPMFQTPLSLHSLANSTHLLFEGSEEPFPAHTSSGTSSGHKHQPQESPDMQPLGTAQAGPAGSTSDQQPSSPVAGAADQGSEPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPKEHALKNTSDLCISPLQGTPELRTALHGPFSQGPRKSCSLGALDKACVSSLDYRNAQTAPSPAVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLELRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEEEWVNIQYPDQAEDSPEAPPDNLPPYSALLPEHAGAQGIQPNWTSAQVPEPDAIKESLLYLYADRTWIPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEAAHWRDLVRKVLASYLESVQEAVTLAGSLDENCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELSKRLSHRNYLLESPHKFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNTVS

Target ORF information:

RefSeq Version	XM_006247527.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pleckstrin homology and RUN domain containing M1 (Plekhm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247527.2

ORF Insert Sequence:

ATGTGCAGTG CCCTGGAGGC CGTGTTCATC CATGGCTTGC ATGCCAAGTA CATCCGTGCT 
GAGGCTGGCG GCAAAAGGAA GAAGCACACT CATCAGAAGC CTCTGCCTCA GCCTGTCTTC 
TGGCCCCTTC TGAAAGCTGT CACCCACAAA CACATTATCT CAGACTTGGA GCACCTGGTC 
TTCATCAATA CAGACGTAGG CCGCTGCCGG GCCTGGCTGC GGTTGGCCCT CAATGATGGG 
CTGATGGAGT GCTACCTGAA GTTGCTCCTG CAGGAACCTG CACGGTTGTG CGAGTACTAT 
CAGCCCACGG CCCTGCTTCG AGATGCTGAG GAAGCCGAGT TCCTCCTGAG CTTCCTCCAA 
GGACTAACAT CCTTGTCTTT TGAACTCTCC TACAAGTCAG CCATCTTAAA TGAATGGACA 
CTCACCCCAC TGTCTCTCTC TGGGCTTTGC CCCCTCTCTG AACTGGACCC TCTCACTGTC 
TCTGGTGCAG AACTGCAACG GAAGGAGTCT CTGGATTCCA TTTCCCATTC CTCAGGCTCT 
GAGGACATCG AAGTCCAACA CTCAGGCCAT AAGATCCGCA GGGACAGGAA GCTCACCGCC 
TCTTCCCTCA GCCTGGACAC AGCCAGCTCA TCCCAGCTAT CCTGCAGCCT AAATTCTGAC 
AGCTGCCTGC TCCAAGAGAA TGGTCCCAAG AGTCCAGACC ACTCTGAGGA ACCCATGTCC 
TACGACTCAG ATCTGGGCAC AGCAAATGCT GATGACTCTG ACAGGTCTCT GCAAGAGGTG 
TTGTCGGAAT TCAGCAAAGC CCAGGTAAAT TCTGCACCAA GCAGCGGACC AAGCCAAGAG 
TCAGACACCC CCATGTTCCA GACCCCCCTC TCCCTCCACA GCCTAGCTAA CAGCACACAC 
CTGCTCTTTG AAGGATCTGA AGAGCCCTTT CCTGCCCATA CATCCTCTGG GACTTCAAGT 
GGCCACAAGC ATCAGCCCCA GGAGAGTCCA GACATGCAGC CGCTAGGCAC TGCCCAAGCT 
GGCCCTGCTG GGAGCACTTC AGACCAGCAG CCATCTAGTC CCGTGGCCGG AGCAGCTGAC 
CAAGGAAGTG AGCCTTGGAA GGCCCTGGAA TATGGCAGAG TTGGACCGAA GCTTGTGGTT 
TCCTCTCCTA CGAGTCCGAA AGGCAAGAGC TGGATTTCTG AAGATGACTT CTGCCGACCT 
CCCAAGGAGC ATGCTCTGAA GAACACTTCA GATCTTTGCA TATCTCCTCT GCAAGGGACT 
CCAGAGTTGA GGACTGCTCT CCACGGGCCG TTTTCTCAAG GACCAAGAAA GAGCTGCTCC 
CTGGGGGCCT TAGACAAAGC GTGTGTGTCC TCGCTGGACT ACAGAAATGC CCAGACAGCT 
CCATCTCCAG CGGTGACACA AGACCATAAA AACTTCTGTG TGGTGCATCG AAGGCAGATG 
GGGCTCTCTA ACCCATTCCG GGGCCTCATG AAGCTGGGCA CAGTAGCCCG GCGAGGAGCC 
ATGGGCATCT GGAAGGAATT CTTTTGTGAG CTGTCCCCAC TGGAGCTCCG CCTCTACCTG 
AGTGACGAGG AGCGCACCTG TGTGGAGAGC TGCTCCCTGC TACGGTGTGA GGCCGTGGGA 
CCAGCACACA GCGATGGACG TTTTGAGTTG GTCTTCTCCG GCAAGAAGCT GGCTCTCCGT 
GCCTCCTCCC AGGACGAGGC TGAGGACTGG CTTGACCGTG TGCGGGAGGC TCTGCAGAAG 
GTGCGCCCTC AGCAGGAGGA GGAGTGGGTG AATATCCAGT ACCCAGACCA GGCTGAGGAT 
TCCCCAGAGG CGCCACCAGA CAACCTTCCC CCCTATTCAG CCCTGCTCCC AGAACATGCA 
GGTGCACAGG GAATACAGCC AAACTGGACA TCTGCCCAGG TTCCAGAGCC AGATGCTATC 
AAAGAGTCTC TTTTGTACCT GTATGCAGAC CGGACCTGGA TTCCCTATAT CTTTTCCCTG 
TCCTTGGAGT CTCTGAAATG TTTCCGTGTG AGAAACAATG AGAAGATGCT AAGTGATAGC 
CATGGAGTGG AGACCATCCG GGACATCCTG CCAGACACCA GCCTTGGGGG CCCAGCCTTC 
TTCAAAATCA TCACGGCCAA GGCTGTCCTC AAACTGCAGG CCAAGAACAC TGAGGAGGCC 
GCCCACTGGA GAGACCTAGT CAGGAAAGTG CTGGCGTCTT ACTTAGAGTC AGTTCAGGAG 
GCTGTGACAC TTGCTGGGAG TCTGGATGAA AACTGTCAGG AGGTCCTGAA GTTTGCCACC 
AGGGAGAATG GCTTCCTGCT ACAGTACCTT GTGGCCATCC CGACCGAGAA GGGCCTCGAC 
TCCCAGGGCT GCTTCTGTGC AGGTTGCTCC CGGCAGATCG GCTTCTCCTT TGTACGACCT 
AAGCTCTGTG CCTTCTCTGG CCTATATTAC TGTGACTTCT GCCACCAAGA CGATGCCTCG 
GTGATTCCAG CCAGAATCAT TCATAACTGG GACCTCACAA AGCGCCCGGT CTGCCGGCAG 
GCCCTGAAGT TCCTGGCCCA GATCCGGGCC CAGCCCCTCA TCAACCTGCA GCTGGTGAAC 
GCCTCTCTGT ATGAGCATGT AGAGCGCATG CACCTCATTG GCAGGAGCCG GGAGCAGCTG 
AAACTTCTGG GGGACTACCT GGGCCTGTGC CGAAGTGGTG CTCTGAAGGA GTTGAGCAAA 
AGGCTAAGCC ACAGGAATTA CCTCTTGGAG TCTCCTCACA AATTCAGTGT TGCTGACCTC 
CAGCAGATTG CAGAGGGGGT GTATGAAGGA TTCCTCAAAG CCCTGATTGA ATTCGCCTCC 
CAGCATGTCT ACCACTGTGA CCTGTGCACC CAGCGAGGCT TCATCTGCCA GATCTGCCAC 
CACCAGGACA TCATCTTTCC CTTTGAGTTT GACACCACAG TCAGGTGTGC TGAATGCAGA 
ACTGTCTTCC ATCAGAGCTG CCAGGCTGTG GTGAGGAAGG GCTGTCCCCG CTGTGCCCGC 
CGGCGCAAGT ACCAGGAACA GAACACTGTC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis.
JCI insight1(17)e86330(2016 Oct)
Fujiwara T,Ye S,Castro-Gomes T,Winchell CG,Andrews NW,Voth DE,Varughese KI,Mackintosh SG,Feng Y,Pavlos N,Nakamura T,Manolagas SC,Zhao H

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Plekhm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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