Dhx58 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx58
Entrez Gene ID	303538
Full Name	DEXH-box helicase 58
Synonyms	Lgp2,RGD1310093
General protein information	Preferred Names DEXH-box helicase 58 Names probable ATP-dependent RNA helicase DHX58 DEXH (Asp-Glu-X-His) box polypeptide 58 RNA helicase LGP2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098788.1	NP_001092258.1	probable ATP-dependent RNA helicase DHX58
XM_006247315.3	XP_006247377.1	probable ATP-dependent RNA helicase DHX58 isoform X1
XM_006247314.3	XP_006247376.1	probable ATP-dependent RNA helicase DHX58 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DHX58	XP_005624525.1
	DHX58	XP_001108799.1
Mus musculus (house mouse)	Dhx58	NP_084426.2
Gallus gallus (chicken)	DHX58	XP_003642879.1
Danio rerio (zebrafish)	dhx58	NP_001244086.1
Pan troglodytes (chimpanzee)	DHX58	XP_001167051.1
Bos taurus (cattle)	DHX58	NP_001015545.1
Homo sapiens (human)	DHX58	NP_077024.2
Rattus norvegicus (Norway rat)	Dhx58	NP_001092258.1
Xenopus tropicalis (tropical clawed frog)	dhx58	XP_002939087.2

Gene Ontology
Bibliography

Related articles in PubMed

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Ambivalent role of the innate immune response in rabies virus pathogenesis.
Chopy D, Pothlichet J, Lafage M, M?gret F, Fiette L, Si-Tahar M, Lafon M
Journal of virology85(13)6657-68(2011 Jul)

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Zhang Z, Kim T, Bao M, Facchinetti V, Jung SY, Ghaffari AA, Qin J, Cheng G, Liu YJ
Immunity34(6)866-78(2011 Jun)

RIG-I/MDA5/MAVS are required to signal a protective IFN response in rotavirus-infected intestinal epithelium.
Broquet AH, Hirata Y, McAllister CS, Kagnoff MF
Journal of immunology (Baltimore, Md. : 1950)186(3)1618-26(2011 Feb)

LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses.
Satoh T, Kato H, Kumagai Y, Yoneyama M, Sato S, Matsushita K, Tsujimura T, Fujita T, Akira S, Takeuchi O
Proceedings of the National Academy of Sciences of the United States of America107(4)1512-7(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional analysis of the human LGP2 ortholog.
Title: RNA- and virus-independent inhibition of antiviral signaling by RNA helicase LGP2.

The following Dhx58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33690	NM_001098788.1 Latest version!	Rattus norvegicus DEXH-box helicase 58 (Dhx58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa33690	XM_006247315.3 Latest version!	Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa33690	XM_006247314.3 Latest version!	Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx58 ( NM_001098788.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001092258.1 Rattus norvegicus DEXH-box helicase 58 (Dhx58), mRNA.

Price & Availability↑

CloneID	ORa33690
Clone ID Related Accession (Same CDS sequence)	NM_001098788.1 , XM_006247315.3 , XM_006247314.3
Accession Version	NM_001098788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DHX58
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030475.1, CB544595.1, BP488074.1, CB546277.1 and CR460878.1. On or before Jun 26, 2007 this sequence version replaced XM_220978.4, XM_001081462.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092258.1
CDS	2..2038
Translation	MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVNQHAEEFRRMLDKQWTVTTLSGDMGSRAGFGLLARSHDLLICTAELLHLALKSSEEDEHVELTEFSLIVVDECHHTHKDTIYNTILSRYLEHKLKKAKPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTWRIMSPKNCYSQLLEHNPKPCKQYDLCQRRTQDPFGDLLKKLMKQIHQQLEMPDLKQQFGTQMYEQQVVQLSKDAAEAGLQEQRVYALHLRRYNDALYIHDTVRAWDALNMLQDFYDTERALKTQMVHAERWLLELFDDHRKALAQFAAQGPENPKLEMLEGILLKQFGSPDHTRGIIFTRTRQTASSLLLWLRQQPCLQTVNIKPQMLIGAGNTGQSTHMTQKDQQEVIQEFRDGKLNLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFVATEGSREIKRELTNEALEVLMEQAVAAVQKMDPEEFKAKIQDLQLASLVKRAARAAHRESQQGQFPPDRVQLLCINCMVAVGYGSDLRKVEGTHHVNVNPDFSVYYTTSQNPVVINKVFKDWKPGGIIRCSNCGEVWGFQMIYKSVTLPVLKIRSMLLETPHGKIQAKKWSRVPFSVPDFDILRDCTQSLSDLSLD

Target ORF information:

RefSeq Version	NM_001098788.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEXH-box helicase 58 (Dhx58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098788.1

ORF Insert Sequence:

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx58 ( XM_006247315.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247377.1 Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33690
Clone ID Related Accession (Same CDS sequence)	NM_001098788.1 , XM_006247315.3 , XM_006247314.3
Accession Version	XM_006247315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DHX58 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247377.1
CDS	557..2593
Translation	MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVNQHAEEFRRMLDKQWTVTTLSGDMGSRAGFGLLARSHDLLICTAELLHLALKSSEEDEHVELTEFSLIVVDECHHTHKDTIYNTILSRYLEHKLKKAKPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTWRIMSPKNCYSQLLEHNPKPCKQYDLCQRRTQDPFGDLLKKLMKQIHQQLEMPDLKQQFGTQMYEQQVVQLSKDAAEAGLQEQRVYALHLRRYNDALYIHDTVRAWDALNMLQDFYDTERALKTQMVHAERWLLELFDDHRKALAQFAAQGPENPKLEMLEGILLKQFGSPDHTRGIIFTRTRQTASSLLLWLRQQPCLQTVNIKPQMLIGAGNTGQSTHMTQKDQQEVIQEFRDGKLNLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFVATEGSREIKRELTNEALEVLMEQAVAAVQKMDPEEFKAKIQDLQLASLVKRAARAAHRESQQGQFPPDRVQLLCINCMVAVGYGSDLRKVEGTHHVNVNPDFSVYYTTSQNPVVINKVFKDWKPGGIIRCSNCGEVWGFQMIYKSVTLPVLKIRSMLLETPHGKIQAKKWSRVPFSVPDFDILRDCTQSLSDLSLD

Target ORF information:

RefSeq Version	XM_006247315.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247315.3

ORF Insert Sequence:

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx58 ( XM_006247314.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247376.1 Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X1, mRNA.

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CloneID	ORa33690
Clone ID Related Accession (Same CDS sequence)	NM_001098788.1 , XM_006247315.3 , XM_006247314.3
Accession Version	XM_006247314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DHX58 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247376.1
CDS	568..2604
Translation	MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVNQHAEEFRRMLDKQWTVTTLSGDMGSRAGFGLLARSHDLLICTAELLHLALKSSEEDEHVELTEFSLIVVDECHHTHKDTIYNTILSRYLEHKLKKAKPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTWRIMSPKNCYSQLLEHNPKPCKQYDLCQRRTQDPFGDLLKKLMKQIHQQLEMPDLKQQFGTQMYEQQVVQLSKDAAEAGLQEQRVYALHLRRYNDALYIHDTVRAWDALNMLQDFYDTERALKTQMVHAERWLLELFDDHRKALAQFAAQGPENPKLEMLEGILLKQFGSPDHTRGIIFTRTRQTASSLLLWLRQQPCLQTVNIKPQMLIGAGNTGQSTHMTQKDQQEVIQEFRDGKLNLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFVATEGSREIKRELTNEALEVLMEQAVAAVQKMDPEEFKAKIQDLQLASLVKRAARAAHRESQQGQFPPDRVQLLCINCMVAVGYGSDLRKVEGTHHVNVNPDFSVYYTTSQNPVVINKVFKDWKPGGIIRCSNCGEVWGFQMIYKSVTLPVLKIRSMLLETPHGKIQAKKWSRVPFSVPDFDILRDCTQSLSDLSLD

Target ORF information:

RefSeq Version	XM_006247314.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEXH-box helicase 58 (Dhx58), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247314.3

ORF Insert Sequence:

ATGGAGCTGC GACCCTACCA GTGGGAAGTG ATCTTACCTG CTCTGGAGGG CAAGAACATC 
ATTATATGGC TTCCCACGGG TGCTGGGAAG ACCCGGGCAG CTGCCTTTGT GGCCAAGAGG 
CATCTAGAGA CAGTAGACCG AGGCAAGGTG GTGGTACTGG TTAATAGGGT ACACCTGGTG 
AACCAGCATG CCGAGGAGTT CAGGCGCATG TTGGATAAAC AATGGACCGT GACAACCCTG 
AGTGGGGACA TGGGATCACG AGCTGGTTTT GGCCTATTGG CTCGGAGCCA TGACCTGCTC 
ATCTGTACGG CAGAGTTACT GCATCTGGCG CTGAAGAGCT CTGAGGAGGA TGAACACGTA 
GAGCTCACAG AATTCTCGCT GATTGTGGTG GACGAGTGTC ACCATACCCA CAAGGACACC 
ATCTACAACA CCATCTTGAG CCGGTACCTA GAGCATAAAC TGAAGAAGGC AAAGCCCCTT 
CCCCAGGTCC TGGGTCTCAC AGCCTCCCCA GGCACTGGAG GAGCCACCAA GCTCCAAGGG 
GCCATTGACC ACATCCTACA GCTCTGTGCG AATTTGGATA CGTGGCGCAT CATGTCACCG 
AAGAATTGCT ACTCCCAGCT GCTGGAGCAT AACCCAAAGC CCTGCAAGCA GTATGACCTC 
TGCCAAAGGC GCACGCAGGA TCCTTTTGGA GACTTGCTAA AAAAGCTTAT GAAGCAAATC 
CACCAACAAC TAGAGATGCC TGACCTGAAG CAGCAGTTCG GAACCCAGAT GTATGAGCAG 
CAAGTGGTAC AGTTAAGCAA GGACGCGGCA GAGGCTGGGC TCCAGGAACA GCGGGTGTAT 
GCGCTGCATT TGCGGCGCTA CAATGATGCT CTATATATCC ACGATACGGT CCGTGCGTGG 
GACGCACTTA ACATGTTGCA AGATTTCTAC GACACAGAAC GCGCCCTAAA AACACAGATG 
GTGCATGCTG AGCGCTGGCT GCTGGAGCTG TTTGATGACC ATAGAAAAGC TCTGGCCCAG 
TTTGCAGCCC AAGGTCCTGA GAACCCGAAG TTGGAGATGC TGGAAGGGAT CCTACTGAAG 
CAGTTTGGGA GTCCTGACCA CACTCGGGGT ATCATCTTCA CCAGAACCCG TCAGACTGCT 
TCTTCCCTCC TGCTCTGGCT TCGGCAGCAA CCTTGCCTAC AGACTGTGAA CATCAAGCCC 
CAGATGCTGA TTGGAGCAGG GAACACAGGC CAGAGCACAC ATATGACCCA GAAAGACCAG 
CAAGAGGTGA TCCAGGAGTT CAGGGATGGT AAACTGAACC TTCTAGTGGC CACAAGTGTA 
GCAGAGGAGG GCCTGGATAT CGCTCAGTGC AATGTGGTGG TGCGCTATGG GCTCCTGACC 
AATGAGATCT CCATGGTCCA GGCTCGGGGT CGCGCTCGAG CTGGTCAGAG TGTGTACTCC 
TTCGTGGCTA CAGAGGGCAG TCGGGAGATA AAGCGGGAGC TGACCAATGA GGCACTGGAA 
GTGTTGATGG AGCAGGCAGT GGCCGCTGTC CAGAAAATGG ACCCTGAGGA GTTCAAGGCC 
AAGATCCAGG ACCTTCAGCT AGCATCTCTA GTTAAGCGAG CAGCACGTGC GGCCCATCGG 
GAGAGCCAGC AGGGGCAGTT CCCACCAGAT CGCGTGCAGC TTCTCTGCAT CAACTGTATG 
GTGGCCGTTG GCTACGGGAG TGACCTGCGG AAGGTGGAGG GCACCCACCA CGTCAATGTG 
AACCCCGACT TCTCGGTCTA CTATACCACT TCCCAGAATC CTGTGGTCAT TAACAAGGTC 
TTTAAGGACT GGAAACCTGG AGGAATCATC AGATGCAGTA ACTGTGGAGA GGTCTGGGGC 
TTCCAGATGA TCTACAAATC AGTGACCTTG CCGGTGCTCA AAATCAGAAG CATGCTTCTG 
GAGACCCCTC ACGGGAAGAT CCAGGCCAAA AAGTGGTCCC GAGTGCCTTT CTCAGTACCT 
GACTTCGACA TTCTGCGGGA TTGCACCCAA AGCCTGTCTG ATCTCTCACT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Ambivalent role of the innate immune response in rabies virus pathogenesis.
Journal of virology85(13)6657-68(2011 Jul)
Chopy D,Pothlichet J,Lafage M,M?gret F,Fiette L,Si-Tahar M,Lafon M

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Immunity34(6)866-78(2011 Jun)
Zhang Z,Kim T,Bao M,Facchinetti V,Jung SY,Ghaffari AA,Qin J,Cheng G,Liu YJ

RIG-I/MDA5/MAVS are required to signal a protective IFN response in rotavirus-infected intestinal epithelium.
Journal of immunology (Baltimore, Md. : 1950)186(3)1618-26(2011 Feb)
Broquet AH,Hirata Y,McAllister CS,Kagnoff MF

LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses.
Proceedings of the National Academy of Sciences of the United States of America107(4)1512-7(2010 Jan)
Satoh T,Kato H,Kumagai Y,Yoneyama M,Sato S,Matsushita K,Tsujimura T,Fujita T,Akira S,Takeuchi O

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Dhx58 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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