Lig3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lig3
Entrez Gene ID	303369
Full Name	DNA ligase 3
General protein information	Preferred Names DNA ligase 3 Names DNA ligase 3 DNA ligase (ATP) 3 ligase III, DNA, ATP-dependent
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768022.2	XP_008766244.1	DNA ligase 3 isoform X4
NM_001012011.1	NP_001012011.1	DNA ligase 3
XM_008768023.1	XP_008766245.1	DNA ligase 3 isoform X4
XM_006246990.3	XP_006247052.1	DNA ligase 3 isoform X1
XM_008768021.2	XP_008766243.1	DNA ligase 3 isoform X3
XM_006246989.3	XP_006247051.1	DNA ligase 3 isoform X1
XM_008768020.2	XP_008766242.1	DNA ligase 3 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LIG3	NP_001033196.1
Rattus norvegicus (Norway rat)	Lig3	NP_001012011.1
Gallus gallus (chicken)	LIG3	NP_001006215.1
Homo sapiens (human)	LIG3	NP_039269.2
	LIG3	XP_001113780.1
Canis lupus familiaris (dog)	LIG3	XP_005624869.1
Mus musculus (house mouse)	Lig3	NP_034846.2
Drosophila melanogaster (fruit fly)	lig3	NP_650187.2
Xenopus tropicalis (tropical clawed frog)	lig3	NP_001016351.2
Pan troglodytes (chimpanzee)	LIG3	XP_511409.3
Danio rerio (zebrafish)	lig3	NP_001025345.2

Gene Ontology
Bibliography

Related articles in PubMed

DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells.
Oh S, Harvey A, Zimbric J, Wang Y, Nguyen T, Jackson PJ, Hendrickson EA
DNA repair2197-110(2014 Sep)

Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair.
Akbari M, Keijzers G, Maynard S, Scheibye-Knudsen M, Desler C, Hickson ID, Bohr VA
DNA repair1644-53(2014 Apr)

Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells.
Ruhanen H, Ushakov K, Yasukawa T
Biochimica et biophysica acta1813(12)2000-7(2011 Dec)

DNA polymerases and repair synthesis in NER in human cells.
Lehmann AR
DNA repair10(7)730-3(2011 Jul)

DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.
Simsek D, Brunet E, Wong SY, Katyal S, Gao Y, McKinnon PJ, Lou J, Zhang L, Li J, Rebar EJ, Gregory PD, Holmes MC, Jasin M
PLoS genetics7(6)e1002080(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lig3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lig3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01949	NM_001012011.1 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa01949	XM_008768023.1 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa01949	XM_008768022.2 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa33510	XM_008768021.2 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa33508	XM_006246989.3 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa33508	XM_006246990.3 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa33509	XM_008768020.2 Latest version!	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lig3 ( NM_001012011.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012011.1 Rattus norvegicus DNA ligase 3 (Lig3), mRNA.

Price & Availability↑

CloneID	ORa01949
Clone ID Related Accession (Same CDS sequence)	XM_008768022.2 , NM_001012011.1 , XM_008768023.1
Accession Version	NM_001012011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085959.1. On Feb 11, 2005 this sequence version replaced XM_220766.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642708 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012011.1
CDS	82..2913
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKRRRASRQRGRRAMRTGRR

Target ORF information:

RefSeq Version	NM_001012011.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012011.1

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_008768022.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766244.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa01949
Clone ID Related Accession (Same CDS sequence)	XM_008768022.2 , NM_001012011.1 , XM_008768023.1
Accession Version	XM_008768022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766244.1
CDS	1937..4768
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKRRRASRQRGRRAMRTGRR

Target ORF information:

RefSeq Version	XM_008768022.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768022.2

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_008768023.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766245.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa01949
Clone ID Related Accession (Same CDS sequence)	XM_008768022.2 , NM_001012011.1 , XM_008768023.1
Accession Version	XM_008768023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766245.1
CDS	116..2947
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKRRRASRQRGRRAMRTGRR

Target ORF information:

RefSeq Version	XM_008768023.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768023.1

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGAGGCG GCGGGCCAGC CGGCAAAGAG GAAGGAGAGC TATGCGAACA 
GGCAGGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_008768021.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766243.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33510
Clone ID Related Accession (Same CDS sequence)	XM_008768021.2
Accession Version	XM_008768021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG AGAGAAGCTG 
GCTGTGAAGT CTTCTCCTGT CAAAGTGGGG ATGAAGAGGA AAGCTGCTGA TGAGACCCCA 
GGCCTGACAA AGGCGCTGTT GGATGTCTTC ACTGGGGTGA GGCTCTACTT GCCACCTTCT 
ACACCAGACT TCAGACGGCT CAAACGTTAC TTTGTGGCAT TCAACGGGGA CCTGGTACAG 
GAATTTGACA TGGCCTCAGC CACACATGTG CTGGGTAACA GGGACAAGAA CACTGATGCC 
CAGTTGGTCT CTTCAGAGTG GATTTGGGCA TGCATCCGGA AACGGAGGCT GGTAGCTCCC 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766243.1
CDS	1934..4819
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGEKLAVKSSPVKVGMKRKAADETPGLTKALLDVFTGVRLYLPPSTPDFRRLKRYFVAFNGDLVQEFDMASATHVLGNRDKNTDAQLVSSEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_008768021.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768021.2

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG AGAGAAGCTG 
GCTGTGAAGT CTTCTCCTGT CAAAGTGGGG ATGAAGAGGA AAGCTGCTGA TGAGACCCCA 
GGCCTGACAA AGGCGCTGTT GGATGTCTTC ACTGGGGTGA GGCTCTACTT GCCACCTTCT 
ACACCAGACT TCAGACGGCT CAAACGTTAC TTTGTGGCAT TCAACGGGGA CCTGGTACAG 
GAATTTGACA TGGCCTCAGC CACACATGTG CTGGGTAACA GGGACAAGAA CACTGATGCC 
CAGTTGGTCT CTTCAGAGTG GATTTGGGCA TGCATCCGGA AACGGAGGCT GGTAGCTCCC 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_006246990.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247052.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33508
Clone ID Related Accession (Same CDS sequence)	XM_006246990.3 , XM_006246989.3
Accession Version	XM_006246990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGGCGCT GTTGGATGTC TTCACTGGGG TGAGGCTCTA CTTGCCACCT 
TCTACACCAG ACTTCAGACG GCTCAAACGT TACTTTGTGG CATTCAACGG GGACCTGGTA 
CAGGAATTTG ACATGGCCTC AGCCACACAT GTGCTGGGTA ACAGGGACAA GAACACTGAT 
GCCCAGTTGG TCTCTTCAGA GTGGATTTGG GCATGCATCC GGAAACGGAG GCTGGTAGCT 
CCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247052.1
CDS	101..3109
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKALLDVFTGVRLYLPPSTPDFRRLKRYFVAFNGDLVQEFDMASATHVLGNRDKNTDAQLVSSEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_006246990.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246990.3

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGGCGCT GTTGGATGTC TTCACTGGGG TGAGGCTCTA CTTGCCACCT 
TCTACACCAG ACTTCAGACG GCTCAAACGT TACTTTGTGG CATTCAACGG GGACCTGGTA 
CAGGAATTTG ACATGGCCTC AGCCACACAT GTGCTGGGTA ACAGGGACAA GAACACTGAT 
GCCCAGTTGG TCTCTTCAGA GTGGATTTGG GCATGCATCC GGAAACGGAG GCTGGTAGCT 
CCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_006246989.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247051.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33508
Clone ID Related Accession (Same CDS sequence)	XM_006246990.3 , XM_006246989.3
Accession Version	XM_006246989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGGCGCT GTTGGATGTC TTCACTGGGG TGAGGCTCTA CTTGCCACCT 
TCTACACCAG ACTTCAGACG GCTCAAACGT TACTTTGTGG CATTCAACGG GGACCTGGTA 
CAGGAATTTG ACATGGCCTC AGCCACACAT GTGCTGGGTA ACAGGGACAA GAACACTGAT 
GCCCAGTTGG TCTCTTCAGA GTGGATTTGG GCATGCATCC GGAAACGGAG GCTGGTAGCT 
CCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247051.1
CDS	116..3124
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKALLDVFTGVRLYLPPSTPDFRRLKRYFVAFNGDLVQEFDMASATHVLGNRDKNTDAQLVSSEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_006246989.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246989.3

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCAGCCA AACCGAACAA CTCTGAGCAA 
GACCCCTCAA GCCCTGCTCC TAAGACCAGT CTGTCCGCAA GTAAATGTGA CCCTAAGCAC 
AAAGATTGTC TACTACGAGA ATTCCGGAAG CTGTGTGCCA TGGTAGCTGA AAATCCTAGC 
TATAACACCA AGACCCAGAT CATCCACGAC TTCTTGCAGA AAGGCTCCAC AGGAGATGGC 
TTCCGAGGCG ATGTGTACCT GACAGTGAAG CTGCTGCTGC CTGGTGTCAT CAAGAGTGTG 
TACAACTTGA ATGATAAGCA GATAGTGAAA CTGTTCAGCC GAATTTTTAA CTGCAACCCA 
GATGATATGG CCCGGGACCT AGAACAGGGT GACGTGTCCG AGACGATCAG AGTCTTCTTT 
GAGCAGAGCA AGTCTTTTCC CCCAGCTGCC AAAAGCCTCC TCACCATCCA GGAGGTGGAT 
GCATTTCTTC TGCACCTCTC CAAGCTCACC AAAGAGGATG AGCAGCAGCA GGCCCTGCAG 
GACATTGCTT CCAGGTGTAC AGCCAATGAC CTTAAGTGCA TTATCCGGCT GATCAAACAC 
GATCTGAAGA TGAACTCGGG TGCAAAACAC GTGTTAGATG CCCTTGACCC CAATGCTTAT 
GAAGCCTTCA AAGCATCACG CAACCTGCAG GATGTGGTGG AGCGGGTCCT TCACAATGAG 
CAGGAGGTGG AGAAGGACCC AGGCCGGCGT CGGGCTCTGA GTGTCCAGGC CTCATTGATG 
ACACCTGTGC AACCCATGCT GGCTGAGGCC TGCAAGTCCA TTGAGTATGC AATGAAGAAG 
TGTCCCAATG GCATGTTCTC TGAGATCAAG TACGATGGTG AGCGAGTCCA GGTGCATAAG 
AAGGGAGACC ATTTCAGCTA CTTCAGCCGC AGTCTCAAGC CTGTCTTGCC TCACAAGGTG 
GCCCACTTTA AGGACTATAT TCCCAAAGCG TTTCCTGGGG GTCAAAGCAT GATCTTGGAC 
TCTGAAGTGC TCCTGATTGA TAACAACACA GGCAAACCAC TGCCCTTTGG GACCCTGGGA 
GTACACAAGA AAGCTGCTTT CCAGGATGCT AATGTCTGCC TGTTTGTTTT TGATTGTATC 
TACTTTAATG ATGTCAGCTT GATGGACAGG CCTCTCTGTG AGCGACGGAA GTTTCTTCAT 
GATAACATGG TTGAAATCCG TAACCGGATC ATGTTCTCAG AAATGAAGCA AGTCACAAAA 
GCTTCAGACC TGGCTGACAT GATAAACCGG GTGATCCGAG AGGGCCTGGA AGGTTTGGTG 
CTAAAGGATG TGAAGGGTAC ATATGAGCCT GGAAAACGAC ACTGGCTGAA AGTTAAGAAA 
GACTATTTGA ACGAGGGGGC CATGGCTGAT ACAGCTGATC TGGTGGTTCT TGGGGCCTTC 
TATGGGCAAG GGAGCAAAGG TGGAATGATG TCCATCTTCC TCATGGGCTG CTATGACCCC 
GACAGCCAGA AGTGGTGCAC TGTTACAAAA TGTGCTGGAG GCCATGACGA TGCCACACTT 
GCCCGCCTGC AGAAAGAGCT AGATATGGTG AAGATTAGCA AGGATCCTAG CAAGATACCC 
AGCTGGCTGA AGATCAACAA GATCTACTAT CCTGACTTTA TCGTCCCAGA CCCAAAGAAA 
GCTGCTGTGT GGGAGATCAC AGGAGCAGAA TTCTCTCGAT CTGAGGCGCA TACTGCTGAT 
GGGATCTCCA TCCGATTTCC TCGATGCACC CGAATCCGGG ATGACAAGGA CTGGAAATCT 
GCCACTAACC TCCCCCAACT CAAGGAACTG TACCAGCTGT CCAAAGACAA GGCAGACTTC 
GCTGTGGTGG CTGGAGATGA GGGGAGCTCC ACTACTGGAG GCAGCAATGG TGAGAATGAG 
GGCACTGCTG GGTCTACTGT GCCCCGCAAG GGCCCCAAGG GACCCCCCAG TAAGTCCTCT 
GCCAGTGCCA AGAAGACTGA ACAGAAGCTG AATGATCCCA GTAGCAGAGG TGGAGAGAAG 
CTGGCTGTGA AGTCTTCTCC TGTCAAAGTG GGGATGAAGA GGAAAGCTGC TGATGAGACC 
CCAGGCCTGA CAAAGGCGCT GTTGGATGTC TTCACTGGGG TGAGGCTCTA CTTGCCACCT 
TCTACACCAG ACTTCAGACG GCTCAAACGT TACTTTGTGG CATTCAACGG GGACCTGGTA 
CAGGAATTTG ACATGGCCTC AGCCACACAT GTGCTGGGTA ACAGGGACAA GAACACTGAT 
GCCCAGTTGG TCTCTTCAGA GTGGATTTGG GCATGCATCC GGAAACGGAG GCTGGTAGCT 
CCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig3 ( XM_008768020.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766242.1 Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X3, mRNA.

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CloneID	ORa33509
Clone ID Related Accession (Same CDS sequence)	XM_008768020.2
Accession Version	XM_008768020.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCCAAAC CGAACAACTC TGAGCAAGAC 
CCCTCAAGCC CTGCTCCTAA GACCAGTCTG TCCGCAAGTA AATGTGACCC TAAGCACAAA 
GATTGTCTAC TACGAGAATT CCGGAAGCTG TGTGCCATGG TAGCTGAAAA TCCTAGCTAT 
AACACCAAGA CCCAGATCAT CCACGACTTC TTGCAGAAAG GCTCCACAGG AGATGGCTTC 
CGAGGCGATG TGTACCTGAC AGTGAAGCTG CTGCTGCCTG GTGTCATCAA GAGTGTGTAC 
AACTTGAATG ATAAGCAGAT AGTGAAACTG TTCAGCCGAA TTTTTAACTG CAACCCAGAT 
GATATGGCCC GGGACCTAGA ACAGGGTGAC GTGTCCGAGA CGATCAGAGT CTTCTTTGAG 
CAGAGCAAGT CTTTTCCCCC AGCTGCCAAA AGCCTCCTCA CCATCCAGGA GGTGGATGCA 
TTTCTTCTGC ACCTCTCCAA GCTCACCAAA GAGGATGAGC AGCAGCAGGC CCTGCAGGAC 
ATTGCTTCCA GGTGTACAGC CAATGACCTT AAGTGCATTA TCCGGCTGAT CAAACACGAT 
CTGAAGATGA ACTCGGGTGC AAAACACGTG TTAGATGCCC TTGACCCCAA TGCTTATGAA 
GCCTTCAAAG CATCACGCAA CCTGCAGGAT GTGGTGGAGC GGGTCCTTCA CAATGAGCAG 
GAGGTGGAGA AGGACCCAGG CCGGCGTCGG GCTCTGAGTG TCCAGGCCTC ATTGATGACA 
CCTGTGCAAC CCATGCTGGC TGAGGCCTGC AAGTCCATTG AGTATGCAAT GAAGAAGTGT 
CCCAATGGCA TGTTCTCTGA GATCAAGTAC GATGGTGAGC GAGTCCAGGT GCATAAGAAG 
GGAGACCATT TCAGCTACTT CAGCCGCAGT CTCAAGCCTG TCTTGCCTCA CAAGGTGGCC 
CACTTTAAGG ACTATATTCC CAAAGCGTTT CCTGGGGGTC AAAGCATGAT CTTGGACTCT 
GAAGTGCTCC TGATTGATAA CAACACAGGC AAACCACTGC CCTTTGGGAC CCTGGGAGTA 
CACAAGAAAG CTGCTTTCCA GGATGCTAAT GTCTGCCTGT TTGTTTTTGA TTGTATCTAC 
TTTAATGATG TCAGCTTGAT GGACAGGCCT CTCTGTGAGC GACGGAAGTT TCTTCATGAT 
AACATGGTTG AAATCCGTAA CCGGATCATG TTCTCAGAAA TGAAGCAAGT CACAAAAGCT 
TCAGACCTGG CTGACATGAT AAACCGGGTG ATCCGAGAGG GCCTGGAAGG TTTGGTGCTA 
AAGGATGTGA AGGGTACATA TGAGCCTGGA AAACGACACT GGCTGAAAGT TAAGAAAGAC 
TATTTGAACG AGGGGGCCAT GGCTGATACA GCTGATCTGG TGGTTCTTGG GGCCTTCTAT 
GGGCAAGGGA GCAAAGGTGG AATGATGTCC ATCTTCCTCA TGGGCTGCTA TGACCCCGAC 
AGCCAGAAGT GGTGCACTGT TACAAAATGT GCTGGAGGCC ATGACGATGC CACACTTGCC 
CGCCTGCAGA AAGAGCTAGA TATGGTGAAG ATTAGCAAGG ATCCTAGCAA GATACCCAGC 
TGGCTGAAGA TCAACAAGAT CTACTATCCT GACTTTATCG TCCCAGACCC AAAGAAAGCT 
GCTGTGTGGG AGATCACAGG AGCAGAATTC TCTCGATCTG AGGCGCATAC TGCTGATGGG 
ATCTCCATCC GATTTCCTCG ATGCACCCGA ATCCGGGATG ACAAGGACTG GAAATCTGCC 
ACTAACCTCC CCCAACTCAA GGAACTGTAC CAGCTGTCCA AAGACAAGGC AGACTTCGCT 
GTGGTGGCTG GAGATGAGGG GAGCTCCACT ACTGGAGGCA GCAATGGTGA GAATGAGGGC 
ACTGCTGGGT CTACTGTGCC CCGCAAGGGC CCCAAGGGAC CCCCCAGTAA GTCCTCTGCC 
AGTGCCAAGA AGACTGAACA GAAGCTGAAT GATCCCAGTA GCAGAGGTGG AGAGAAGCTG 
GCTGTGAAGT CTTCTCCTGT CAAAGTGGGG ATGAAGAGGA AAGCTGCTGA TGAGACCCCA 
GGCCTGACAA AGGCGCTGTT GGATGTCTTC ACTGGGGTGA GGCTCTACTT GCCACCTTCT 
ACACCAGACT TCAGACGGCT CAAACGTTAC TTTGTGGCAT TCAACGGGGA CCTGGTACAG 
GAATTTGACA TGGCCTCAGC CACACATGTG CTGGGTAACA GGGACAAGAA CACTGATGCC 
CAGTTGGTCT CTTCAGAGTG GATTTGGGCA TGCATCCGGA AACGGAGGCT GGTAGCTCCC 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766242.1
CDS	1937..4942
Translation	MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRATHLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVTSPVKGASFITSTNPRKFSGFSAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPSYNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKVGMKRKAADETPGLTKALLDVFTGVRLYLPPSTPDFRRLKRYFVAFNGDLVQEFDMASATHVLGNRDKNTDAQLVSSEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_008768020.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 3 (Lig3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768020.2

ORF Insert Sequence:

ATGACTTTGG CCTTTAAGAC CCTCTTCCCG AGAAACCTCT GTGCCCTTGG CAGAAAAGAA 
CTGTGCCTGT TTTCAGAACA GCATCACTGG CCTGCCATAA GACAGTTCAG CCAGTGGTCG 
GAAACAAATC TCCTTTGTGG GTGCTGCCTC CTCCAGAGAA GAAAGCCTGT CCTATCTTTC 
CAGAGAGGTC ATCTAAGACC ACGTGCCACC CACCTTATTT CGTGGTCAGG GTCGCATGTG 
GGACTCTGTA CTGGCCCCTG TGAGATGGCC GAACAGCGGT TCTGTGTGGA CTATGCCAAG 
CGGGGCACAG CTGGCTGCAA GAAATGCAAG GAGAAGATTG TAAAGGGCGT ATGCCGCATT 
GGCAAAGTGG TACCCAATCC CTTCTCAGAG TCTGGGGGTG ATATGAAAGA GTGGTACCAC 
ATTAAGTGCA TGTTTGAGAA ACTGGAGCGG GCGCGGGCCA CCACAAAAAA AATTGAAGAC 
CTCACAGAGC TGGAAGGCTG GGAAGAGCTG GAAGATAATG AAAAGGAACA GATAAGCCAG 
CACATTGCAG ACCTGTCTTC CAAGACAGCT GCCACACCGA AGAAGAAAGC TACTGTCCAG 
GCTAAGCTGA CAACCACTGG CCAAGTGACA TCACCAGTGA AAGGCGCTTC GTTTATTACC 
AGTACCAATC CCCGGAAATT TTCTGGCTTT TCAGCCAAAC CGAACAACTC TGAGCAAGAC 
CCCTCAAGCC CTGCTCCTAA GACCAGTCTG TCCGCAAGTA AATGTGACCC TAAGCACAAA 
GATTGTCTAC TACGAGAATT CCGGAAGCTG TGTGCCATGG TAGCTGAAAA TCCTAGCTAT 
AACACCAAGA CCCAGATCAT CCACGACTTC TTGCAGAAAG GCTCCACAGG AGATGGCTTC 
CGAGGCGATG TGTACCTGAC AGTGAAGCTG CTGCTGCCTG GTGTCATCAA GAGTGTGTAC 
AACTTGAATG ATAAGCAGAT AGTGAAACTG TTCAGCCGAA TTTTTAACTG CAACCCAGAT 
GATATGGCCC GGGACCTAGA ACAGGGTGAC GTGTCCGAGA CGATCAGAGT CTTCTTTGAG 
CAGAGCAAGT CTTTTCCCCC AGCTGCCAAA AGCCTCCTCA CCATCCAGGA GGTGGATGCA 
TTTCTTCTGC ACCTCTCCAA GCTCACCAAA GAGGATGAGC AGCAGCAGGC CCTGCAGGAC 
ATTGCTTCCA GGTGTACAGC CAATGACCTT AAGTGCATTA TCCGGCTGAT CAAACACGAT 
CTGAAGATGA ACTCGGGTGC AAAACACGTG TTAGATGCCC TTGACCCCAA TGCTTATGAA 
GCCTTCAAAG CATCACGCAA CCTGCAGGAT GTGGTGGAGC GGGTCCTTCA CAATGAGCAG 
GAGGTGGAGA AGGACCCAGG CCGGCGTCGG GCTCTGAGTG TCCAGGCCTC ATTGATGACA 
CCTGTGCAAC CCATGCTGGC TGAGGCCTGC AAGTCCATTG AGTATGCAAT GAAGAAGTGT 
CCCAATGGCA TGTTCTCTGA GATCAAGTAC GATGGTGAGC GAGTCCAGGT GCATAAGAAG 
GGAGACCATT TCAGCTACTT CAGCCGCAGT CTCAAGCCTG TCTTGCCTCA CAAGGTGGCC 
CACTTTAAGG ACTATATTCC CAAAGCGTTT CCTGGGGGTC AAAGCATGAT CTTGGACTCT 
GAAGTGCTCC TGATTGATAA CAACACAGGC AAACCACTGC CCTTTGGGAC CCTGGGAGTA 
CACAAGAAAG CTGCTTTCCA GGATGCTAAT GTCTGCCTGT TTGTTTTTGA TTGTATCTAC 
TTTAATGATG TCAGCTTGAT GGACAGGCCT CTCTGTGAGC GACGGAAGTT TCTTCATGAT 
AACATGGTTG AAATCCGTAA CCGGATCATG TTCTCAGAAA TGAAGCAAGT CACAAAAGCT 
TCAGACCTGG CTGACATGAT AAACCGGGTG ATCCGAGAGG GCCTGGAAGG TTTGGTGCTA 
AAGGATGTGA AGGGTACATA TGAGCCTGGA AAACGACACT GGCTGAAAGT TAAGAAAGAC 
TATTTGAACG AGGGGGCCAT GGCTGATACA GCTGATCTGG TGGTTCTTGG GGCCTTCTAT 
GGGCAAGGGA GCAAAGGTGG AATGATGTCC ATCTTCCTCA TGGGCTGCTA TGACCCCGAC 
AGCCAGAAGT GGTGCACTGT TACAAAATGT GCTGGAGGCC ATGACGATGC CACACTTGCC 
CGCCTGCAGA AAGAGCTAGA TATGGTGAAG ATTAGCAAGG ATCCTAGCAA GATACCCAGC 
TGGCTGAAGA TCAACAAGAT CTACTATCCT GACTTTATCG TCCCAGACCC AAAGAAAGCT 
GCTGTGTGGG AGATCACAGG AGCAGAATTC TCTCGATCTG AGGCGCATAC TGCTGATGGG 
ATCTCCATCC GATTTCCTCG ATGCACCCGA ATCCGGGATG ACAAGGACTG GAAATCTGCC 
ACTAACCTCC CCCAACTCAA GGAACTGTAC CAGCTGTCCA AAGACAAGGC AGACTTCGCT 
GTGGTGGCTG GAGATGAGGG GAGCTCCACT ACTGGAGGCA GCAATGGTGA GAATGAGGGC 
ACTGCTGGGT CTACTGTGCC CCGCAAGGGC CCCAAGGGAC CCCCCAGTAA GTCCTCTGCC 
AGTGCCAAGA AGACTGAACA GAAGCTGAAT GATCCCAGTA GCAGAGGTGG AGAGAAGCTG 
GCTGTGAAGT CTTCTCCTGT CAAAGTGGGG ATGAAGAGGA AAGCTGCTGA TGAGACCCCA 
GGCCTGACAA AGGCGCTGTT GGATGTCTTC ACTGGGGTGA GGCTCTACTT GCCACCTTCT 
ACACCAGACT TCAGACGGCT CAAACGTTAC TTTGTGGCAT TCAACGGGGA CCTGGTACAG 
GAATTTGACA TGGCCTCAGC CACACATGTG CTGGGTAACA GGGACAAGAA CACTGATGCC 
CAGTTGGTCT CTTCAGAGTG GATTTGGGCA TGCATCCGGA AACGGAGGCT GGTAGCTCCC 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells.
DNA repair2197-110(2014 Sep)
Oh S,Harvey A,Zimbric J,Wang Y,Nguyen T,Jackson PJ,Hendrickson EA

Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair.
DNA repair1644-53(2014 Apr)
Akbari M,Keijzers G,Maynard S,Scheibye-Knudsen M,Desler C,Hickson ID,Bohr VA

Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells.
Biochimica et biophysica acta1813(12)2000-7(2011 Dec)
Ruhanen H,Ushakov K,Yasukawa T

DNA polymerases and repair synthesis in NER in human cells.
DNA repair10(7)730-3(2011 Jul)
Lehmann AR

DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.
PLoS genetics7(6)e1002080(2011 Jun)
Simsek D,Brunet E,Wong SY,Katyal S,Gao Y,McKinnon PJ,Lou J,Zhang L,Li J,Rebar EJ,Gregory PD,Holmes MC,Jasin M

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Lig3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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