Cntrob cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cntrob
Entrez Gene ID	303240
Full Name	centrobin, centriole duplication and spindle assembly protein
Synonyms	Lip8,RGD1307488
General protein information	Preferred Names centrobin, centriole duplication and spindle assembly protein Names centrobin LYST-interacting protein 8 centrobin, centrosomal BRCA2 interacting protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767825.2	XP_008766047.1	centrobin isoform X2
NM_001134645.1	NP_001128117.1	centrobin
XM_006246701.3	XP_006246763.1	centrobin isoform X1
XM_006246702.2	XP_006246764.1	centrobin isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNTROB	XP_523825.3
Rattus norvegicus (Norway rat)	Cntrob	NP_001128117.1
Homo sapiens (human)	CNTROB	NP_001032221.1
	CNTROB	XP_001111510.1
Canis lupus familiaris (dog)	CNTROB	XP_546602.2
Mus musculus (house mouse)	Cntrob	NP_766148.2

Gene Ontology
Bibliography

Related articles in PubMed

CEP295 interacts with microtubules and is required for centriole elongation.
Chang CW, Hsu WB, Tsai JJ, Tang CJ, Tang TK
Journal of cell science129(13)2501-13(2016 07)

Overexpression of full-length centrobin rescues limb malformation but not male fertility of the hypodactylous (hd) rats.
Li?ka F, Gosele C, Popova E, Chyl?kov? B, K?enov? D, K?en V, Bader M, Tres LL, Hubner N, Kierszenbaum AL
PloS one8(4)e60859(2013)

Centrobin-tubulin interaction is required for centriole elongation and stability.
Gudi R, Zou C, Li J, Gao Q
The Journal of cell biology193(4)711-25(2011 May)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

Rat hd mutation reveals an essential role of centrobin in spermatid head shaping and assembly of the head-tail coupling apparatus.
Liska F, Gosele C, Rivkin E, Tres L, Cardoso MC, Domaing P, Krejc? E, Snajdr P, Lee-Kirsch MA, de Rooij DG, Rooij DG, Kren V, Krenov? D, Kierszenbaum AL, Hubner N
Biology of reproduction81(6)1196-205(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

centrobin is essential for normal patterning of the limb autopod
Title: Overexpression of full-length centrobin rescues limb malformation but not male fertility of the hypodactylous (hd) rats.

Cntrob may represent a novel candidate gene for presently unexplained hereditary forms of teratozoospermia and the "easily decapitated sperm syndrome" in humans.
Title: Rat hd mutation reveals an essential role of centrobin in spermatid head shaping and assembly of the head-tail coupling apparatus.

The following Cntrob gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cntrob cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03372	NM_001134645.1 Latest version!	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa03372	XM_006246701.3 Latest version!	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa33313	XM_006246702.2 Latest version!	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46001	XM_008767825.2 Latest version!	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cntrob ( NM_001134645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128117.1 Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), mRNA.

Price & Availability↑

CloneID	ORa03372
Clone ID Related Accession (Same CDS sequence)	NM_001134645.1 , XM_006246701.3
Accession Version	NM_001134645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	centrobin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF532342.1. On or before Sep 4, 2008 this sequence version replaced XM_001078458.1, XM_220600.4. ##Evidence-Data-START## Transcript exon combination :: EF532342.1, EF532344.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGCTGCTGG AGACAGCACC TCAGAGCAAC 
GAAAACCCCT CTGTTGACCT GTTGGCCCCT AAGTCTGGTT CTCGGGCTGT CTCGTCTTGG 
GAGGAAGCCC CTCAAGTGCC TCGACTTCCA CCTCCTGTCC ACAAAACTAA AGTTCCCTTA 
GCCATGGCTT CTAGTCTTTT CAGGGTCCAT GAGCTTCCTT CATCCAATCT GCAAGGCAGT 
GGTGTCAGCA CTGGCTCCCC AGAGAAAGGT ACAGATGGGC TCAGCCCCCC AAGGCAGCTG 
ATGGAAGTGT CTGAGCTCTT ACGTCTGTAC CAGGCTCGGG GTTGGGGTGC TCTGCCTGCT 
GAGGACCTGC TACTGTACCT GAAGAGACTG GAACATGGCG GGACTGATGG CCAAGGGGAG 
CTTGTCCCCA GAAGAAACAC AGACTCCCGT TTGGGTGAGA TCACCCGGAA AGAGATCCCC 
TCCCAAGCTG TCCCTCGACG CCTTGCTTCA GCCCCTAAGA CTGAAAAGCC TGCTCGGAAG 
AAAAGTGGGC ATCCTGGCCC CAGCATGAGG AGTCGGGGTG GCATCTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128117.1
CDS	225..2918
Translation	MATAAPSPNSPLRPEDLLTDSSEPPGLNQVSSEVTSQLYTSLHLSRQAEATARAQLYLASTSSPPNEGIDSLAQELSRSLSVGLENNLKKKDGSKHIFEMESVRGQLQTMLHTSRDMAYRTGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPTNPPDCQGLRDALDSELTRRKHCERHIQSLQTRVLELQQQLAVAVAADHKKDLMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEERQAAELTRSKQQETVTRLEQSLSEAMEALNREQESTRLQQREREALEEERQALAMRLEVEQQQCQALQQERDEAKAGQLSEHRRLEALQVALQEERQAWVKQEQQLKERLQALQEEGQAQLEREKGTSQREAQAAREAQQQLALLQSEVRRLEGDLDTVRRERDALQLEMSLVQARYESQRIQMESELAVQLEQRVTERLAEVQENSLRQAASLRDHHRKQLQELSGQHQQELAAQLAQFKVEMAEREERQQQVAQDYELRLAREQARVRDLKSGNQQLEEQRVELVERLQAMLQAHWDEANQLLSTTLLPPNPQAPLAEPSSPGPLEPEKGERRIWAMPPMAVALKPVLQQGREGRADLPGAPSVLCSTSPDLSLLLGPSFQSQNSFQPLEPKPDVAPPAVGPFSALEAFTADHRAEHPFPEEDPGPEGDGPLKAGLPPASQLEGLKNFLQQLLETAPQSNENPSVDLLAPKSGSRAVSSWEEAPQVPRLPPPVHKTKVPLAMASSLFRVHELPSSNLQGSGVSTGSPEKGTDGLSPPRQLMEVSELLRLYQARGWGALPAEDLLLYLKRLEHGGTDGQGELVPRRNTDSRLGEITRKEIPSQAVPRRLASAPKTEKPARKKSGHPGPSMRSRGGIWR

Target ORF information:

RefSeq Version	NM_001134645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134645.1

ORF Insert Sequence:

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGCTGCTGG AGACAGCACC TCAGAGCAAC 
GAAAACCCCT CTGTTGACCT GTTGGCCCCT AAGTCTGGTT CTCGGGCTGT CTCGTCTTGG 
GAGGAAGCCC CTCAAGTGCC TCGACTTCCA CCTCCTGTCC ACAAAACTAA AGTTCCCTTA 
GCCATGGCTT CTAGTCTTTT CAGGGTCCAT GAGCTTCCTT CATCCAATCT GCAAGGCAGT 
GGTGTCAGCA CTGGCTCCCC AGAGAAAGGT ACAGATGGGC TCAGCCCCCC AAGGCAGCTG 
ATGGAAGTGT CTGAGCTCTT ACGTCTGTAC CAGGCTCGGG GTTGGGGTGC TCTGCCTGCT 
GAGGACCTGC TACTGTACCT GAAGAGACTG GAACATGGCG GGACTGATGG CCAAGGGGAG 
CTTGTCCCCA GAAGAAACAC AGACTCCCGT TTGGGTGAGA TCACCCGGAA AGAGATCCCC 
TCCCAAGCTG TCCCTCGACG CCTTGCTTCA GCCCCTAAGA CTGAAAAGCC TGCTCGGAAG 
AAAAGTGGGC ATCCTGGCCC CAGCATGAGG AGTCGGGGTG GCATCTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cntrob ( XM_006246701.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246763.1 Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa03372
Clone ID Related Accession (Same CDS sequence)	NM_001134645.1 , XM_006246701.3
Accession Version	XM_006246701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	centrobin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGCTGCTGG AGACAGCACC TCAGAGCAAC 
GAAAACCCCT CTGTTGACCT GTTGGCCCCT AAGTCTGGTT CTCGGGCTGT CTCGTCTTGG 
GAGGAAGCCC CTCAAGTGCC TCGACTTCCA CCTCCTGTCC ACAAAACTAA AGTTCCCTTA 
GCCATGGCTT CTAGTCTTTT CAGGGTCCAT GAGCTTCCTT CATCCAATCT GCAAGGCAGT 
GGTGTCAGCA CTGGCTCCCC AGAGAAAGGT ACAGATGGGC TCAGCCCCCC AAGGCAGCTG 
ATGGAAGTGT CTGAGCTCTT ACGTCTGTAC CAGGCTCGGG GTTGGGGTGC TCTGCCTGCT 
GAGGACCTGC TACTGTACCT GAAGAGACTG GAACATGGCG GGACTGATGG CCAAGGGGAG 
CTTGTCCCCA GAAGAAACAC AGACTCCCGT TTGGGTGAGA TCACCCGGAA AGAGATCCCC 
TCCCAAGCTG TCCCTCGACG CCTTGCTTCA GCCCCTAAGA CTGAAAAGCC TGCTCGGAAG 
AAAAGTGGGC ATCCTGGCCC CAGCATGAGG AGTCGGGGTG GCATCTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246763.1
CDS	611..3304
Translation	MATAAPSPNSPLRPEDLLTDSSEPPGLNQVSSEVTSQLYTSLHLSRQAEATARAQLYLASTSSPPNEGIDSLAQELSRSLSVGLENNLKKKDGSKHIFEMESVRGQLQTMLHTSRDMAYRTGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPTNPPDCQGLRDALDSELTRRKHCERHIQSLQTRVLELQQQLAVAVAADHKKDLMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEERQAAELTRSKQQETVTRLEQSLSEAMEALNREQESTRLQQREREALEEERQALAMRLEVEQQQCQALQQERDEAKAGQLSEHRRLEALQVALQEERQAWVKQEQQLKERLQALQEEGQAQLEREKGTSQREAQAAREAQQQLALLQSEVRRLEGDLDTVRRERDALQLEMSLVQARYESQRIQMESELAVQLEQRVTERLAEVQENSLRQAASLRDHHRKQLQELSGQHQQELAAQLAQFKVEMAEREERQQQVAQDYELRLAREQARVRDLKSGNQQLEEQRVELVERLQAMLQAHWDEANQLLSTTLLPPNPQAPLAEPSSPGPLEPEKGERRIWAMPPMAVALKPVLQQGREGRADLPGAPSVLCSTSPDLSLLLGPSFQSQNSFQPLEPKPDVAPPAVGPFSALEAFTADHRAEHPFPEEDPGPEGDGPLKAGLPPASQLEGLKNFLQQLLETAPQSNENPSVDLLAPKSGSRAVSSWEEAPQVPRLPPPVHKTKVPLAMASSLFRVHELPSSNLQGSGVSTGSPEKGTDGLSPPRQLMEVSELLRLYQARGWGALPAEDLLLYLKRLEHGGTDGQGELVPRRNTDSRLGEITRKEIPSQAVPRRLASAPKTEKPARKKSGHPGPSMRSRGGIWR

Target ORF information:

RefSeq Version	XM_006246701.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246701.3

ORF Insert Sequence:

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGCTGCTGG AGACAGCACC TCAGAGCAAC 
GAAAACCCCT CTGTTGACCT GTTGGCCCCT AAGTCTGGTT CTCGGGCTGT CTCGTCTTGG 
GAGGAAGCCC CTCAAGTGCC TCGACTTCCA CCTCCTGTCC ACAAAACTAA AGTTCCCTTA 
GCCATGGCTT CTAGTCTTTT CAGGGTCCAT GAGCTTCCTT CATCCAATCT GCAAGGCAGT 
GGTGTCAGCA CTGGCTCCCC AGAGAAAGGT ACAGATGGGC TCAGCCCCCC AAGGCAGCTG 
ATGGAAGTGT CTGAGCTCTT ACGTCTGTAC CAGGCTCGGG GTTGGGGTGC TCTGCCTGCT 
GAGGACCTGC TACTGTACCT GAAGAGACTG GAACATGGCG GGACTGATGG CCAAGGGGAG 
CTTGTCCCCA GAAGAAACAC AGACTCCCGT TTGGGTGAGA TCACCCGGAA AGAGATCCCC 
TCCCAAGCTG TCCCTCGACG CCTTGCTTCA GCCCCTAAGA CTGAAAAGCC TGCTCGGAAG 
AAAAGTGGGC ATCCTGGCCC CAGCATGAGG AGTCGGGGTG GCATCTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cntrob ( XM_006246702.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246764.1 Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X3, mRNA.

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CloneID	ORa33313
Clone ID Related Accession (Same CDS sequence)	XM_006246702.2
Accession Version	XM_006246702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	centrobin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGGTTCTCG GGCTGTCTCG TCTTGGGAGG 
AAGCCCCTCA AGTGCCTCGA CTTCCACCTC CTGTCCACAA AACTAAAGTT CCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246764.1
CDS	873..3089
Translation	MATAAPSPNSPLRPEDLLTDSSEPPGLNQVSSEVTSQLYTSLHLSRQAEATARAQLYLASTSSPPNEGIDSLAQELSRSLSVGLENNLKKKDGSKHIFEMESVRGQLQTMLHTSRDMAYRTGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPTNPPDCQGLRDALDSELTRRKHCERHIQSLQTRVLELQQQLAVAVAADHKKDLMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEERQAAELTRSKQQETVTRLEQSLSEAMEALNREQESTRLQQREREALEEERQALAMRLEVEQQQCQALQQERDEAKAGQLSEHRRLEALQVALQEERQAWVKQEQQLKERLQALQEEGQAQLEREKGTSQREAQAAREAQQQLALLQSEVRRLEGDLDTVRRERDALQLEMSLVQARYESQRIQMESELAVQLEQRVTERLAEVQENSLRQAASLRDHHRKQLQELSGQHQQELAAQLAQFKVEMAEREERQQQVAQDYELRLAREQARVRDLKSGNQQLEEQRVELVERLQAMLQAHWDEANQLLSTTLLPPNPQAPLAEPSSPGPLEPEKGERRIWAMPPMAVALKPVLQQGREGRADLPGAPSVLCSTSPDLSLLLGPSFQSQNSFQPLEPKPDVAPPAVGPFSALEAFTADHRAEHPFPEEDPGPEGDGPLKAGLPPASQLEGLKNFLQQVLGLSRLGRKPLKCLDFHLLSTKLKFP

Target ORF information:

RefSeq Version	XM_006246702.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246702.2

ORF Insert Sequence:

ATGGCTACAG CAGCCCCCAG CCCCAATTCC CCTCTCCGGC CAGAGGACCT CCTGACCGAT 
TCATCAGAGC CTCCTGGGTT GAACCAGGTG TCCTCGGAGG TGACCTCGCA GCTGTATACT 
TCTTTGCATC TCAGCCGGCA AGCGGAGGCC ACAGCCCGAG CCCAGCTGTA TTTAGCTTCC 
ACCTCCTCAC CTCCTAATGA GGGCATAGAC AGCTTGGCTC AAGAACTGAG TCGCAGCTTG 
TCAGTTGGAT TGGAAAACAA CTTGAAGAAA AAGGATGGGT CTAAGCATAT CTTTGAGATG 
GAAAGCGTTC GGGGCCAGCT CCAGACCATG CTCCATACCT CACGTGATAT GGCCTATCGA 
ACTGGCTCAG AGAGACGGGA AGAGGACTCC TTTGACAGTG ACAGCACTGC TACCTTGCTC 
AACACCCGGC CTCTGCAAGA CCTGTCTCCA TCGAGCTCAG CCCAGGCACT GGAAGAGTTG 
TTTCCCCGAT ATACCAGCCT TCGACCAGGG CCCCCCACCA ACCCCCCAGA TTGTCAGGGG 
CTGAGGGATG CACTGGACTC AGAGCTTACA CGCCGAAAGC ATTGCGAGCG CCACATTCAG 
AGCCTACAGA CTCGAGTGCT AGAGTTACAA CAACAGCTAG CTGTGGCCGT GGCTGCTGAC 
CACAAGAAAG ATCTCATGAT TGAACAGCTA GACAAGACTC TGGCTCGAGT GGTAGAGGGC 
TGGAATCGGC ATGAGGCTGA GCGGACAGAG GTGCTGAGGG GACTTCAGGA GGAGCGCCAG 
GCAGCAGAAC TCACCAGAAG CAAGCAGCAG GAGACAGTAA CCCGCCTGGA ACAAAGCCTT 
TCTGAGGCCA TGGAAGCCCT GAATCGTGAG CAGGAGAGTA CCAGGCTACA GCAGAGGGAA 
AGAGAGGCAC TGGAAGAAGA ACGGCAAGCC CTGGCCATGA GGTTGGAGGT GGAGCAGCAG 
CAGTGCCAGG CCCTCCAGCA AGAGCGGGAT GAGGCCAAGG CTGGGCAGCT CAGTGAGCAC 
AGGAGGCTGG AGGCCCTTCA AGTGGCTCTG CAAGAGGAGA GGCAGGCCTG GGTCAAGCAG 
GAACAGCAGC TGAAGGAACG CCTTCAGGCG CTGCAGGAGG AGGGCCAGGC TCAGCTGGAA 
AGAGAGAAGG GGACCAGCCA GAGGGAGGCA CAGGCAGCCC GGGAGGCCCA GCAGCAGTTG 
GCACTGCTGC AGTCTGAGGT GCGGCGGCTG GAAGGGGACC TGGACACGGT GCGGAGAGAG 
AGAGACGCAC TGCAGCTGGA GATGAGCTTG GTCCAGGCCA GATATGAGAG TCAGCGGATC 
CAGATGGAGT CAGAGCTGGC TGTGCAGCTG GAGCAGCGGG TGACCGAGCG GCTGGCGGAG 
GTTCAAGAGA ACAGCCTGCG GCAGGCCGCC TCCCTCAGGG ACCATCACAG GAAGCAGCTA 
CAGGAACTCA GTGGACAGCA CCAACAGGAG TTGGCTGCCC AGCTGGCTCA GTTCAAGGTG 
GAAATGGCTG AACGAGAGGA GCGCCAGCAG CAGGTTGCTC AGGACTATGA GCTCAGGCTG 
GCAAGGGAGC AAGCTCGTGT GCGTGACCTG AAGAGCGGCA ACCAGCAGCT GGAGGAGCAG 
CGGGTGGAGC TGGTAGAGCG GCTCCAAGCC ATGCTGCAGG CCCACTGGGA TGAGGCCAAC 
CAGCTGCTCA GTACCACACT TCTGCCTCCC AACCCTCAGG CCCCTCTTGC TGAGCCCTCC 
AGCCCAGGGC CTCTGGAGCC AGAGAAGGGG GAGAGGAGGA TCTGGGCCAT GCCTCCCATG 
GCTGTGGCCC TGAAACCTGT GCTGCAGCAA GGCCGAGAAG GGAGGGCTGA TCTGCCGGGA 
GCTCCCTCTG TTCTCTGTAG CACCTCACCA GATCTGAGCC TCCTGCTGGG ACCCTCTTTC 
CAGAGCCAGA ACTCCTTTCA GCCCCTGGAA CCCAAGCCTG ATGTGGCTCC ACCTGCAGTT 
GGACCCTTCT CTGCATTGGA GGCTTTCACT GCTGATCATA GAGCAGAGCA CCCATTTCCT 
GAGGAGGACC CTGGGCCTGA AGGTGATGGG CCCCTAAAGG CAGGGCTGCC ACCTGCTTCT 
CAGCTGGAAG GCCTCAAGAA CTTTCTGCAG CAGGTTCTCG GGCTGTCTCG TCTTGGGAGG 
AAGCCCCTCA AGTGCCTCGA CTTCCACCTC CTGTCCACAA AACTAAAGTT CCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cntrob ( XM_008767825.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766047.1 Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa46001
Clone ID Related Accession (Same CDS sequence)	XM_008767825.2
Accession Version	XM_008767825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	centrobin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCG TTCGGGGCCA GCTCCAGACC ATGCTCCATA CCTCACGTGA TATGGCCTAT 
CGAACTGGCT CAGAGAGACG GGAAGAGGAC TCCTTTGACA GTGACAGCAC TGCTACCTTG 
CTCAACACCC GGCCTCTGCA AGACCTGTCT CCATCGAGCT CAGCCCAGGC ACTGGAAGAG 
TTGTTTCCCC GATATACCAG CCTTCGACCA GGGCCCCCCA CCAACCCCCC AGATTGTCAG 
GGGCTGAGGG ATGCACTGGA CTCAGAGCTT ACACGCCGAA AGCATTGCGA GCGCCACATT 
CAGAGCCTAC AGACTCGAGT GCTAGAGTTA CAACAACAGC TAGCTGTGGC CGTGGCTGCT 
GACCACAAGA AAGATCTCAT GATTGAACAG CTAGACAAGA CTCTGGCTCG AGTGGTAGAG 
GGCTGGAATC GGCATGAGGC TGAGCGGACA GAGGTGCTGA GGGGACTTCA GGAGGAGCGC 
CAGGCAGCAG AACTCACCAG AAGCAAGCAG CAGGAGACAG TAACCCGCCT GGAACAAAGC 
CTTTCTGAGG CCATGGAAGC CCTGAATCGT GAGCAGGAGA GTACCAGGCT ACAGCAGAGG 
GAAAGAGAGG CACTGGAAGA AGAACGGCAA GCCCTGGCCA TGAGGTTGGA GGTGGAGCAG 
CAGCAGTGCC AGGCCCTCCA GCAAGAGCGG GATGAGGCCA AGGCTGGGCA GCTCAGTGAG 
CACAGGAGGC TGGAGGCCCT TCAAGTGGCT CTGCAAGAGG AGAGGCAGGC CTGGGTCAAG 
CAGGAACAGC AGCTGAAGGA ACGCCTTCAG GCGCTGCAGG AGGAGGGCCA GGCTCAGCTG 
GAAAGAGAGA AGGGGACCAG CCAGAGGGAG GCACAGGCAG CCCGGGAGGC CCAGCAGCAG 
TTGGCACTGC TGCAGTCTGA GGTGCGGCGG CTGGAAGGGG ACCTGGACAC GGTGCGGAGA 
GAGAGAGACG CACTGCAGCT GGAGATGAGC TTGGTCCAGG CCAGATATGA GAGTCAGCGG 
ATCCAGATGG AGTCAGAGCT GGCTGTGCAG CTGGAGCAGC GGGTGACCGA GCGGCTGGCG 
GAGGTTCAAG AGAACAGCCT GCGGCAGGCC GCCTCCCTCA GGGACCATCA CAGGAAGCAG 
CTACAGGAAC TCAGTGGACA GCACCAACAG GAGTTGGCTG CCCAGCTGGC TCAGTTCAAG 
GTGGAAATGG CTGAACGAGA GGAGCGCCAG CAGCAGGTTG CTCAGGACTA TGAGCTCAGG 
CTGGCAAGGG AGCAAGCTCG TGTGCGTGAC CTGAAGAGCG GCAACCAGCA GCTGGAGGAG 
CAGCGGGTGG AGCTGGTAGA GCGGCTCCAA GCCATGCTGC AGGCCCACTG GGATGAGGCC 
AACCAGCTGC TCAGTACCAC ACTTCTGCCT CCCAACCCTC AGGCCCCTCT TGCTGAGCCC 
TCCAGCCCAG GGCCTCTGGA GCCAGAGAAG GGGGAGAGGA GGATCTGGGC CATGCCTCCC 
ATGGCTGTGG CCCTGAAACC TGTGCTGCAG CAAGGCCGAG AAGGGAGGGC TGATCTGCCG 
GGAGCTCCCT CTGTTCTCTG TAGCACCTCA CCAGATCTGA GCCTCCTGCT GGGACCCTCT 
TTCCAGAGCC AGAACTCCTT TCAGCCCCTG GAACCCAAGC CTGATGTGGC TCCACCTGCA 
GTTGGACCCT TCTCTGCATT GGAGGCTTTC ACTGCTGATC ATAGAGCAGA GCACCCATTT 
CCTGAGGAGG ACCCTGGGCC TGAAGGTGAT GGGCCCCTAA AGGCAGGGCT GCCACCTGCT 
TCTCAGCTGG AAGGCCTCAA GAACTTTCTG CAGCAGCTGC TGGAGACAGC ACCTCAGAGC 
AACGAAAACC CCTCTGTTGA CCTGTTGGCC CCTAAGTCTG GTTCTCGGGC TGTCTCGTCT 
TGGGAGGAAG CCCCTCAAGT GCCTCGACTT CCACCTCCTG TCCACAAAAC TAAAGTTCCC 
TTAGCCATGG CTTCTAGTCT TTTCAGGGTC CATGAGCTTC CTTCATCCAA TCTGCAAGGC 
AGTGGTGTCA GCACTGGCTC CCCAGAGAAA GGTACAGATG GGCTCAGCCC CCCAAGGCAG 
CTGATGGAAG TGTCTGAGCT CTTACGTCTG TACCAGGCTC GGGGTTGGGG TGCTCTGCCT 
GCTGAGGACC TGCTACTGTA CCTGAAGAGA CTGGAACATG GCGGGACTGA TGGCCAAGGG 
GAGCTTGTCC CCAGAAGAAA CACAGACTCC CGTTTGGGTG AGATCACCCG GAAAGAGATC 
CCCTCCCAAG CTGTCCCTCG ACGCCTTGCT TCAGCCCCTA AGACTGAAAA GCCTGCTCGG 
AAGAAAAGTG GGCATCCTGG CCCCAGCATG AGGAGTCGGG GTGGCATCTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766047.1
CDS	226..2622
Translation	MESVRGQLQTMLHTSRDMAYRTGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPTNPPDCQGLRDALDSELTRRKHCERHIQSLQTRVLELQQQLAVAVAADHKKDLMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEERQAAELTRSKQQETVTRLEQSLSEAMEALNREQESTRLQQREREALEEERQALAMRLEVEQQQCQALQQERDEAKAGQLSEHRRLEALQVALQEERQAWVKQEQQLKERLQALQEEGQAQLEREKGTSQREAQAAREAQQQLALLQSEVRRLEGDLDTVRRERDALQLEMSLVQARYESQRIQMESELAVQLEQRVTERLAEVQENSLRQAASLRDHHRKQLQELSGQHQQELAAQLAQFKVEMAEREERQQQVAQDYELRLAREQARVRDLKSGNQQLEEQRVELVERLQAMLQAHWDEANQLLSTTLLPPNPQAPLAEPSSPGPLEPEKGERRIWAMPPMAVALKPVLQQGREGRADLPGAPSVLCSTSPDLSLLLGPSFQSQNSFQPLEPKPDVAPPAVGPFSALEAFTADHRAEHPFPEEDPGPEGDGPLKAGLPPASQLEGLKNFLQQLLETAPQSNENPSVDLLAPKSGSRAVSSWEEAPQVPRLPPPVHKTKVPLAMASSLFRVHELPSSNLQGSGVSTGSPEKGTDGLSPPRQLMEVSELLRLYQARGWGALPAEDLLLYLKRLEHGGTDGQGELVPRRNTDSRLGEITRKEIPSQAVPRRLASAPKTEKPARKKSGHPGPSMRSRGGIWR

Target ORF information:

RefSeq Version	XM_008767825.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus centrobin, centriole duplication and spindle assembly protein (Cntrob), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767825.2

ORF Insert Sequence:

ATGGAAAGCG TTCGGGGCCA GCTCCAGACC ATGCTCCATA CCTCACGTGA TATGGCCTAT 
CGAACTGGCT CAGAGAGACG GGAAGAGGAC TCCTTTGACA GTGACAGCAC TGCTACCTTG 
CTCAACACCC GGCCTCTGCA AGACCTGTCT CCATCGAGCT CAGCCCAGGC ACTGGAAGAG 
TTGTTTCCCC GATATACCAG CCTTCGACCA GGGCCCCCCA CCAACCCCCC AGATTGTCAG 
GGGCTGAGGG ATGCACTGGA CTCAGAGCTT ACACGCCGAA AGCATTGCGA GCGCCACATT 
CAGAGCCTAC AGACTCGAGT GCTAGAGTTA CAACAACAGC TAGCTGTGGC CGTGGCTGCT 
GACCACAAGA AAGATCTCAT GATTGAACAG CTAGACAAGA CTCTGGCTCG AGTGGTAGAG 
GGCTGGAATC GGCATGAGGC TGAGCGGACA GAGGTGCTGA GGGGACTTCA GGAGGAGCGC 
CAGGCAGCAG AACTCACCAG AAGCAAGCAG CAGGAGACAG TAACCCGCCT GGAACAAAGC 
CTTTCTGAGG CCATGGAAGC CCTGAATCGT GAGCAGGAGA GTACCAGGCT ACAGCAGAGG 
GAAAGAGAGG CACTGGAAGA AGAACGGCAA GCCCTGGCCA TGAGGTTGGA GGTGGAGCAG 
CAGCAGTGCC AGGCCCTCCA GCAAGAGCGG GATGAGGCCA AGGCTGGGCA GCTCAGTGAG 
CACAGGAGGC TGGAGGCCCT TCAAGTGGCT CTGCAAGAGG AGAGGCAGGC CTGGGTCAAG 
CAGGAACAGC AGCTGAAGGA ACGCCTTCAG GCGCTGCAGG AGGAGGGCCA GGCTCAGCTG 
GAAAGAGAGA AGGGGACCAG CCAGAGGGAG GCACAGGCAG CCCGGGAGGC CCAGCAGCAG 
TTGGCACTGC TGCAGTCTGA GGTGCGGCGG CTGGAAGGGG ACCTGGACAC GGTGCGGAGA 
GAGAGAGACG CACTGCAGCT GGAGATGAGC TTGGTCCAGG CCAGATATGA GAGTCAGCGG 
ATCCAGATGG AGTCAGAGCT GGCTGTGCAG CTGGAGCAGC GGGTGACCGA GCGGCTGGCG 
GAGGTTCAAG AGAACAGCCT GCGGCAGGCC GCCTCCCTCA GGGACCATCA CAGGAAGCAG 
CTACAGGAAC TCAGTGGACA GCACCAACAG GAGTTGGCTG CCCAGCTGGC TCAGTTCAAG 
GTGGAAATGG CTGAACGAGA GGAGCGCCAG CAGCAGGTTG CTCAGGACTA TGAGCTCAGG 
CTGGCAAGGG AGCAAGCTCG TGTGCGTGAC CTGAAGAGCG GCAACCAGCA GCTGGAGGAG 
CAGCGGGTGG AGCTGGTAGA GCGGCTCCAA GCCATGCTGC AGGCCCACTG GGATGAGGCC 
AACCAGCTGC TCAGTACCAC ACTTCTGCCT CCCAACCCTC AGGCCCCTCT TGCTGAGCCC 
TCCAGCCCAG GGCCTCTGGA GCCAGAGAAG GGGGAGAGGA GGATCTGGGC CATGCCTCCC 
ATGGCTGTGG CCCTGAAACC TGTGCTGCAG CAAGGCCGAG AAGGGAGGGC TGATCTGCCG 
GGAGCTCCCT CTGTTCTCTG TAGCACCTCA CCAGATCTGA GCCTCCTGCT GGGACCCTCT 
TTCCAGAGCC AGAACTCCTT TCAGCCCCTG GAACCCAAGC CTGATGTGGC TCCACCTGCA 
GTTGGACCCT TCTCTGCATT GGAGGCTTTC ACTGCTGATC ATAGAGCAGA GCACCCATTT 
CCTGAGGAGG ACCCTGGGCC TGAAGGTGAT GGGCCCCTAA AGGCAGGGCT GCCACCTGCT 
TCTCAGCTGG AAGGCCTCAA GAACTTTCTG CAGCAGCTGC TGGAGACAGC ACCTCAGAGC 
AACGAAAACC CCTCTGTTGA CCTGTTGGCC CCTAAGTCTG GTTCTCGGGC TGTCTCGTCT 
TGGGAGGAAG CCCCTCAAGT GCCTCGACTT CCACCTCCTG TCCACAAAAC TAAAGTTCCC 
TTAGCCATGG CTTCTAGTCT TTTCAGGGTC CATGAGCTTC CTTCATCCAA TCTGCAAGGC 
AGTGGTGTCA GCACTGGCTC CCCAGAGAAA GGTACAGATG GGCTCAGCCC CCCAAGGCAG 
CTGATGGAAG TGTCTGAGCT CTTACGTCTG TACCAGGCTC GGGGTTGGGG TGCTCTGCCT 
GCTGAGGACC TGCTACTGTA CCTGAAGAGA CTGGAACATG GCGGGACTGA TGGCCAAGGG 
GAGCTTGTCC CCAGAAGAAA CACAGACTCC CGTTTGGGTG AGATCACCCG GAAAGAGATC 
CCCTCCCAAG CTGTCCCTCG ACGCCTTGCT TCAGCCCCTA AGACTGAAAA GCCTGCTCGG 
AAGAAAAGTG GGCATCCTGG CCCCAGCATG AGGAGTCGGG GTGGCATCTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CEP295 interacts with microtubules and is required for centriole elongation.
Journal of cell science129(13)2501-13(2016 07)
Chang CW,Hsu WB,Tsai JJ,Tang CJ,Tang TK

Overexpression of full-length centrobin rescues limb malformation but not male fertility of the hypodactylous (hd) rats.
PloS one8(4)e60859(2013)
Li?ka F,Gosele C,Popova E,Chyl?kov? B,K?enov? D,K?en V,Bader M,Tres LL,Hubner N,Kierszenbaum AL

Centrobin-tubulin interaction is required for centriole elongation and stability.
The Journal of cell biology193(4)711-25(2011 May)
Gudi R,Zou C,Li J,Gao Q

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

Rat hd mutation reveals an essential role of centrobin in spermatid head shaping and assembly of the head-tail coupling apparatus.
Biology of reproduction81(6)1196-205(2009 Dec)
Liska F,Gosele C,Rivkin E,Tres L,Cardoso MC,Domaing P,Krejc? E,Snajdr P,Lee-Kirsch MA,de Rooij DG,Rooij DG,Kren V,Krenov? D,Kierszenbaum AL,Hubner N

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Cntrob cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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