Map2k3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map2k3
Entrez Gene ID	303200
Full Name	mitogen activated protein kinase kinase 3
Synonyms	Mek3,Mkk3
General protein information	Preferred Names mitogen activated protein kinase kinase 3 Names dual specificity mitogen-activated protein kinase kinase 3 dual specificity mitogen-activated protein kinase kinase 3-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100674.1	NP_001094144.1	dual specificity mitogen-activated protein kinase kinase 3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MAP2K3	NP_001012805.2
Drosophila melanogaster (fruit fly)	lic	NP_477162.1
Homo sapiens (human)	MAP2K3	NP_659731.1
Rattus norvegicus (Norway rat)	Map2k3	NP_001094144.1
	MAP2K3	XP_001104193.1
Canis lupus familiaris (dog)	MAP2K3	XP_546666.2
Bos taurus (cattle)	MAP2K3	NP_001077162.1
Mus musculus (house mouse)	Map2k3	NP_032954.1
Pan troglodytes (chimpanzee)	MAP2K3	XP_001150751.1
Rattus norvegicus (Norway rat)	LOC100911550	XP_003750889.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000310	XP_310813.5

Gene Ontology
Bibliography

Related articles in PubMed

Matrine-Type Alkaloids Inhibit Advanced Glycation End Products Induced Reactive Oxygen Species-Mediated Apoptosis of Aortic Endothelial Cells In Vivo and In Vitro by Targeting MKK3 and p38MAPK Signaling.
Liu Z, Lv Y, Zhang Y, Liu F, Zhu L, Pan S, Qiu C, Guo Y, Yang T, Wang J
Journal of the American Heart Association6(12)(2017 Dec)

Redox-dependent dimerization of p38? mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3.
Bassi R, Burgoyne JR, DeNicola GF, Rudyk O, DeSantis V, Charles RL, Eaton P, Marber MS
The Journal of biological chemistry292(39)16161-16173(2017 09)

MAPK kinase 3 is a tumor suppressor with reduced copy number in breast cancer.
MacNeil AJ, Jiao SC, McEachern LA, Yang YJ, Dennis A, Yu H, Xu Z, Marshall JS, Lin TJ
Cancer research74(1)162-72(2014 Jan)

The nNOS-p38MAPK pathway is mediated by NOS1AP during neuronal death.
Li LL, Ginet V, Liu X, Vergun O, Tuittila M, Mathieu M, Bonny C, Puyal J, Truttmann AC, Courtney MJ
The Journal of neuroscience : the official journal of the Society for Neuroscience33(19)8185-201(2013 May)

miR-20a represses endothelial cell migration by targeting MKK3 and inhibiting p38 MAP kinase activation in response to VEGF.
Pin AL, Houle F, Guillonneau M, Paquet ER, Simard MJ, Huot J
Angiogenesis15(4)593-608(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Mapk14/p38alpha is activated and forms cystine disulfide-bound heterodimer with Map2k3/Mkk3 in cardiomyocytes and isolated hearts during oxidative stress (exposure to hydrogen peroxide). (Mapk14, mitogen-activated protein kinase 14; Mkk3 = mitogen-activated protein kinase kinase 3)
Title: Redox-dependent dimerization of p38? mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3.

Superoxide anion is an important signaling molecule for activating the MKK3/6-p38 cascade, which is requisite for inducing tumor necrosis factor (TNF)alpha in microglia.
Title: Superoxide anion contributes to the induction of tumor necrosis factor alpha (TNF?) through activation of the MKK3/6-p38 MAPK cascade in rat microglia.

Rap1, MKK3/6, and p38 MAPK have a role in the induction of mGluR-dependent LTD by directly coupling to receptor trafficking machineries to facilitate the loss of synaptic AMPA receptors [map2k3, also called MKK3]
Title: Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term depression.

The following Map2k3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map2k3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05104	NM_001100674.1 Latest version!	Rattus norvegicus mitogen activated protein kinase kinase 3 (Map2k3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map2k3 ( NM_001100674.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094144.1 Rattus norvegicus mitogen activated protein kinase kinase 3 (Map2k3), mRNA.

Price & Availability↑

CloneID	ORa05104
Clone ID Related Accession (Same CDS sequence)	NM_001100674.1
Accession Version	NM_001100674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-21
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity mitogen-activated protein kinase kinase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DY311082.1, BC160839.1 and BC100095.1. On or before Sep 4, 2008 this sequence version replaced XM_239239.3, XM_001077724.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC160839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCGC CCGCCGCCAG CCCGCCGGCC AGCTTGCCTC AGACCAAAGG AAAATCCAAA 
AGGAAAAGGG ATTTACGGAT ATCCTGTGTG TCCAAGCCAC CCGTGTCCAA CCCCACACCC 
CCCCGGAACC TGGACTCCCG GACCTTCATC ACTATCGGAG ACAGAAACTT CGAAGTGGAG 
GCTGATGACT TGGTGACCAT CTCAGAGCTG GGCCGCGGAG CCTATGGGGT GGTGGAGAAG 
GTGCGGCATG CTCAGAGTGG TACCATCATG GCTGTCAAGC GCATCCGGGC CACTGTGAAC 
TCACAGGAGC AGAAACGTCT GCTTATGGAC CTAGACATCA ACATGCGCAC TGTCGACTGC 
TTCTATACTG TCACCTTCTA TGGTGCCCTC TTTAGAGAGG GGGATGTGTG GATCTGCATG 
GAACTCATGG ACACGTCCCT GGATAAGTTC TACCGGAAGG TGCTAGAGAA GAACATGAAA 
ATTCCGGAAG ACATTCTGGG GGAGATTGCT GTATCCATCG TGCGGGCCCT GGAGCACCTA 
CACAGCAAGC TGTCTGTGAT CCACAGAGAT GTGAAGCCTT CCAATGTCCT CATCAACAAA 
GAGGGGCACG TGAAGATGTG CGACTTTGGC ATCAGTGGCT ACCTGGTGGA CTCTGTGGCA 
AAGACAATGG ATGCTGGCTG CAAGCCTTAC ATGGCCCCTG AGAGGATCAA CCCTGAGCTG 
AATCAGAAGG GCTACAACGT CAAGTCTGAT GTCTGGAGCC TTGGCATCAC CATGATTGAG 
ATGGCCATTC TGCGATTCCC TTACGAGTCT TGGGGGACAC CGTTCCAGCA GCTGAAGCAG 
GTGGTGGAGG AGCCATCCCC ACAGCTCCCA GCAGACCAGT TCTCCCCTGA GTTTGTGGAC 
TTCACAAGCC AGTGCCTCAG GAAGAATCCT GCGGAACGCA TGAGCTACCT GGAGCTGATG 
GAACACCCGT TCTTCACCTT GCACAAAACT AAGAAGACGG ACATTGCTGC CTTTGTGAAG 
GAGATCCTGG GAGAGGATTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094144.1
CDS	163..1206
Translation	MESPAASPPASLPQTKGKSKRKRDLRISCVSKPPVSNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLEKNMKIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADQFSPEFVDFTSQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

Target ORF information:

RefSeq Version	NM_001100674.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen activated protein kinase kinase 3 (Map2k3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100674.1

ORF Insert Sequence:

ATGGAGTCGC CCGCCGCCAG CCCGCCGGCC AGCTTGCCTC AGACCAAAGG AAAATCCAAA 
AGGAAAAGGG ATTTACGGAT ATCCTGTGTG TCCAAGCCAC CCGTGTCCAA CCCCACACCC 
CCCCGGAACC TGGACTCCCG GACCTTCATC ACTATCGGAG ACAGAAACTT CGAAGTGGAG 
GCTGATGACT TGGTGACCAT CTCAGAGCTG GGCCGCGGAG CCTATGGGGT GGTGGAGAAG 
GTGCGGCATG CTCAGAGTGG TACCATCATG GCTGTCAAGC GCATCCGGGC CACTGTGAAC 
TCACAGGAGC AGAAACGTCT GCTTATGGAC CTAGACATCA ACATGCGCAC TGTCGACTGC 
TTCTATACTG TCACCTTCTA TGGTGCCCTC TTTAGAGAGG GGGATGTGTG GATCTGCATG 
GAACTCATGG ACACGTCCCT GGATAAGTTC TACCGGAAGG TGCTAGAGAA GAACATGAAA 
ATTCCGGAAG ACATTCTGGG GGAGATTGCT GTATCCATCG TGCGGGCCCT GGAGCACCTA 
CACAGCAAGC TGTCTGTGAT CCACAGAGAT GTGAAGCCTT CCAATGTCCT CATCAACAAA 
GAGGGGCACG TGAAGATGTG CGACTTTGGC ATCAGTGGCT ACCTGGTGGA CTCTGTGGCA 
AAGACAATGG ATGCTGGCTG CAAGCCTTAC ATGGCCCCTG AGAGGATCAA CCCTGAGCTG 
AATCAGAAGG GCTACAACGT CAAGTCTGAT GTCTGGAGCC TTGGCATCAC CATGATTGAG 
ATGGCCATTC TGCGATTCCC TTACGAGTCT TGGGGGACAC CGTTCCAGCA GCTGAAGCAG 
GTGGTGGAGG AGCCATCCCC ACAGCTCCCA GCAGACCAGT TCTCCCCTGA GTTTGTGGAC 
TTCACAAGCC AGTGCCTCAG GAAGAATCCT GCGGAACGCA TGAGCTACCT GGAGCTGATG 
GAACACCCGT TCTTCACCTT GCACAAAACT AAGAAGACGG ACATTGCTGC CTTTGTGAAG 
GAGATCCTGG GAGAGGATTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Matrine-Type Alkaloids Inhibit Advanced Glycation End Products Induced Reactive Oxygen Species-Mediated Apoptosis of Aortic Endothelial Cells In Vivo and In Vitro by Targeting MKK3 and p38MAPK Signaling.
Journal of the American Heart Association6(12)(2017 Dec)
Liu Z,Lv Y,Zhang Y,Liu F,Zhu L,Pan S,Qiu C,Guo Y,Yang T,Wang J

Redox-dependent dimerization of p38? mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3.
The Journal of biological chemistry292(39)16161-16173(2017 09)
Bassi R,Burgoyne JR,DeNicola GF,Rudyk O,DeSantis V,Charles RL,Eaton P,Marber MS

MAPK kinase 3 is a tumor suppressor with reduced copy number in breast cancer.
Cancer research74(1)162-72(2014 Jan)
MacNeil AJ,Jiao SC,McEachern LA,Yang YJ,Dennis A,Yu H,Xu Z,Marshall JS,Lin TJ

The nNOS-p38MAPK pathway is mediated by NOS1AP during neuronal death.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(19)8185-201(2013 May)
Li LL,Ginet V,Liu X,Vergun O,Tuittila M,Mathieu M,Bonny C,Puyal J,Truttmann AC,Courtney MJ

miR-20a represses endothelial cell migration by targeting MKK3 and inhibiting p38 MAP kinase activation in response to VEGF.
Angiogenesis15(4)593-608(2012 Dec)
Pin AL,Houle F,Guillonneau M,Paquet ER,Simard MJ,Huot J

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Map2k3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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