Alkbh5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alkbh5
Entrez Gene ID	303193
Full Name	alkB homolog 5, RNA demethylase
Synonyms	RGD1309496
General protein information	Preferred Names alkB homolog 5, RNA demethylase Names RNA demethylase ALKBH5 AlkB family member 5, RNA demethylase alkB, alkylation repair homolog 5 alkylated DNA repair protein alkB homolog 5 alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191643.1	NP_001178572.1	RNA demethylase ALKBH5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALKBH5	NP_001192446.1
Mus musculus (house mouse)	Alkbh5	NP_766531.2
Homo sapiens (human)	ALKBH5	NP_060228.3
Pan troglodytes (chimpanzee)	ALKBH5	XP_001158112.2
Canis lupus familiaris (dog)	ALKBH5	XP_851719.1
Xenopus tropicalis (tropical clawed frog)	alkbh5	NP_001008038.1
Danio rerio (zebrafish)	alkbh5	NP_001070855.1
Rattus norvegicus (Norway rat)	Alkbh5	NP_001178572.1
Gallus gallus (chicken)	ALKBH5	XP_001233463.1
	ALKBH5	XP_001093803.2

Gene Ontology
Bibliography

Related articles in PubMed

ALKBH5-dependent m6A demethylation controls splicing and stability of long 3'-UTR mRNAs in male germ cells.
Tang C, Klukovich R, Peng H, Wang Z, Yu T, Zhang Y, Zheng H, Klungland A, Yan W
Proceedings of the National Academy of Sciences of the United States of America115(2)E325-E333(2018 Jan)

Crystal structures of the human RNA demethylase Alkbh5 reveal basis for substrate recognition.
Feng C, Liu Y, Wang G, Deng Z, Zhang Q, Wu W, Tong Y, Cheng C, Chen Z
The Journal of biological chemistry289(17)11571-83(2014 Apr)

ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility.
Zheng G, Dahl JA, Niu Y, Fedorcsak P, Huang CM, Li CJ, V?gb? CB, Shi Y, Wang WL, Song SH, Lu Z, Bosmans RP, Dai Q, Hao YJ, Yang X, Zhao WM, Tong WM, Wang XJ, Bogdan F, Furu K, Fu Y, Jia G, Zhao X, Liu J, Krokan HE, Klungland A, Yang YG, He C
Molecular cell49(1)18-29(2013 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alkbh5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alkbh5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07317	NM_001191643.1 Latest version!	Rattus norvegicus alkB homolog 5, RNA demethylase (Alkbh5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alkbh5 ( NM_001191643.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178572.1 Rattus norvegicus alkB homolog 5, RNA demethylase (Alkbh5), mRNA.

Price & Availability↑

CloneID	ORa07317
Clone ID Related Accession (Same CDS sequence)	NM_001191643.1
Accession Version	NM_001191643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	RNA demethylase ALKBH5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC129460.6. On Sep 17, 2011 this sequence version replaced XM_220525.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FM074585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG CCAGCGGCTA CACGGACCTG CGTGAGAAGC TCAAGTCCAT GACGTCCCGG 
GACAACTACA AGGCAGGCAG CCGGGAGGCC GCCGCCGCTG CAGCCGCTGC TGTGGCCGCC 
GCCGCGGCTG CCGCGGCCGC CGCCGAGCCT TACCCGGTGT CCGGGACCAC CAAACGGAAG 
TACCAGGAGG ACTCAGACCC CGAACGCAGT GACTACGAGG AGCATCAGTT GCAGAAGGAG 
GAGGAGGCGC GCAAGGTGAA GAGCGGCATC CGGCAGATAC GTCTCTTCAG CCAGGATGAG 
TGCTCCAAGA TCGAGGCCCG CATCGACGAG GTGGTGTCCC GCGCTGAGAA GGGCCTGTAC 
AACGAGCACA CTGTGGATCG GGCCCCCCTG CGCAACAAGT ACTTCTTCGG CGAGGGCTAC 
ACGTACGGTG CCCAGCTGCA AAAGCGCGGG CCAGGCCAGG AGCGCCTCTA CCCGCCAGGC 
GACGTCGATG AGATCCCGGA ATGGGTGCAT CAGCTGGTGA TCCAGAAGCT GGTGGAGCAC 
CGCGTCATCC CCGAGGGCTT CGTCAACAGC GCAGTCATCA ACGACTACCA GCCTGGCGGC 
TGCATCGTAT CTCACGTAGA CCCCATCCAC ATCTTCGAGC GCCCGATCGT GTCCGTGTCT 
TTCTTTAGCG ACTCGGCACT TTGCTTCGGT TGCAAGTTCC AGTTCAAGCC CATCCGGGTG 
TCGGAACCTG TGCTTTCCCT GCCGGTGCGC AGGGGGAGCG TGACTGTGCT CAGTGGGTAT 
GCTGCTGATG AGATCACTCA CTGCATACGG CCTCAGGACA TCAAAGAACG CCGGGCGGTC 
ATCATTCTCA GGAAGACAAG ATTAGATGCA CCACGATTGG AAACAAAATC CCTGAGCAGC 
TCCACATTGC CACCCAGCTA TGCTTCAGAT CGTCTGTCAG GAAACACCAG AGACCCTGCG 
CTGAAACCCA AAAGGTCCCA CCGCAAGGCA GACCCTGATG CTGCCCATAG GCCCCGGATC 
CTGGAAATGG ACAAAGAAGA AAACCGGCGG TCTGTGCTTC TGCCCACACA CCGGCGGAGA 
GGGAGTTTTA GCTCTGAGAA CTATTGGCGC AAGTCCTATG AGTCCTCAGA AGATTGCCCA 
GAGGCAGCCA GCAGCCCCAC CCGCAAGGTG AAGATGAGGA GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178572.1
CDS	1..1188
Translation	MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGTTKRKYQEDSDPERSDYEEHQLQKEEEARKVKSGIRQIRLFSQDECSKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSTLPPSYASDRLSGNTRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCPEAASSPTRKVKMRRH

Target ORF information:

RefSeq Version	NM_001191643.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 5, RNA demethylase (Alkbh5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191643.1

ORF Insert Sequence:

ATGGCGGCCG CCAGCGGCTA CACGGACCTG CGTGAGAAGC TCAAGTCCAT GACGTCCCGG 
GACAACTACA AGGCAGGCAG CCGGGAGGCC GCCGCCGCTG CAGCCGCTGC TGTGGCCGCC 
GCCGCGGCTG CCGCGGCCGC CGCCGAGCCT TACCCGGTGT CCGGGACCAC CAAACGGAAG 
TACCAGGAGG ACTCAGACCC CGAACGCAGT GACTACGAGG AGCATCAGTT GCAGAAGGAG 
GAGGAGGCGC GCAAGGTGAA GAGCGGCATC CGGCAGATAC GTCTCTTCAG CCAGGATGAG 
TGCTCCAAGA TCGAGGCCCG CATCGACGAG GTGGTGTCCC GCGCTGAGAA GGGCCTGTAC 
AACGAGCACA CTGTGGATCG GGCCCCCCTG CGCAACAAGT ACTTCTTCGG CGAGGGCTAC 
ACGTACGGTG CCCAGCTGCA AAAGCGCGGG CCAGGCCAGG AGCGCCTCTA CCCGCCAGGC 
GACGTCGATG AGATCCCGGA ATGGGTGCAT CAGCTGGTGA TCCAGAAGCT GGTGGAGCAC 
CGCGTCATCC CCGAGGGCTT CGTCAACAGC GCAGTCATCA ACGACTACCA GCCTGGCGGC 
TGCATCGTAT CTCACGTAGA CCCCATCCAC ATCTTCGAGC GCCCGATCGT GTCCGTGTCT 
TTCTTTAGCG ACTCGGCACT TTGCTTCGGT TGCAAGTTCC AGTTCAAGCC CATCCGGGTG 
TCGGAACCTG TGCTTTCCCT GCCGGTGCGC AGGGGGAGCG TGACTGTGCT CAGTGGGTAT 
GCTGCTGATG AGATCACTCA CTGCATACGG CCTCAGGACA TCAAAGAACG CCGGGCGGTC 
ATCATTCTCA GGAAGACAAG ATTAGATGCA CCACGATTGG AAACAAAATC CCTGAGCAGC 
TCCACATTGC CACCCAGCTA TGCTTCAGAT CGTCTGTCAG GAAACACCAG AGACCCTGCG 
CTGAAACCCA AAAGGTCCCA CCGCAAGGCA GACCCTGATG CTGCCCATAG GCCCCGGATC 
CTGGAAATGG ACAAAGAAGA AAACCGGCGG TCTGTGCTTC TGCCCACACA CCGGCGGAGA 
GGGAGTTTTA GCTCTGAGAA CTATTGGCGC AAGTCCTATG AGTCCTCAGA AGATTGCCCA 
GAGGCAGCCA GCAGCCCCAC CCGCAAGGTG AAGATGAGGA GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ALKBH5-dependent m6A demethylation controls splicing and stability of long 3'-UTR mRNAs in male germ cells.
Proceedings of the National Academy of Sciences of the United States of America115(2)E325-E333(2018 Jan)
Tang C,Klukovich R,Peng H,Wang Z,Yu T,Zhang Y,Zheng H,Klungland A,Yan W

Crystal structures of the human RNA demethylase Alkbh5 reveal basis for substrate recognition.
The Journal of biological chemistry289(17)11571-83(2014 Apr)
Feng C,Liu Y,Wang G,Deng Z,Zhang Q,Wu W,Tong Y,Cheng C,Chen Z

ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility.
Molecular cell49(1)18-29(2013 Jan)
Zheng G,Dahl JA,Niu Y,Fedorcsak P,Huang CM,Li CJ,V?gb? CB,Shi Y,Wang WL,Song SH,Lu Z,Bosmans RP,Dai Q,Hao YJ,Yang X,Zhao WM,Tong WM,Wang XJ,Bogdan F,Furu K,Fu Y,Jia G,Zhao X,Liu J,Krokan HE,Klungland A,Yang YG,He C

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Alkbh5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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