Igsf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Igsf1
Entrez Gene ID	302822
Full Name	immunoglobulin superfamily, member 1
Synonyms	Inhbp
General protein information	Preferred Names immunoglobulin superfamily, member 1 Names immunoglobulin superfamily member 1 inhibin-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq36
Summary	a putative inhibin coreceptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008773564.2	XP_008771786.1	immunoglobulin superfamily member 1 isoform X6
XM_008773563.1	XP_008771785.1	immunoglobulin superfamily member 1 isoform X1
XM_006257557.2	XP_006257619.1	immunoglobulin superfamily member 1 isoform X1
XM_006257559.1	XP_006257621.1	immunoglobulin superfamily member 1 isoform X3
XM_006257561.1	XP_006257623.1	immunoglobulin superfamily member 1 isoform X5
XM_006257558.1	XP_006257620.1	immunoglobulin superfamily member 1 isoform X2
NM_175763.2	NP_786939.1	immunoglobulin superfamily member 1 precursor
XM_006257560.1	XP_006257622.1	immunoglobulin superfamily member 1 isoform X4

Pathways from BioSystems

Homologies

	IGSF1	XP_001095381.1
Canis lupus familiaris (dog)	IGSF1	XP_538173.3
Mus musculus (house mouse)	Igsf1	NP_808259.2
Rattus norvegicus (Norway rat)	Igsf1	NP_786939.1
Homo sapiens (human)	IGSF1	NP_001546.2
Gallus gallus (chicken)	IGSF1	NP_001138698.2

Gene Ontology
Bibliography

Related articles in PubMed

The short mRNA isoform of the immunoglobulin superfamily, member 1 gene encodes an intracellular glycoprotein.
Wang Y, Br?l? E, Silander T, Bak B, Joustra SD, Bernard DJ
PloS one12(7)e0180731(2017)

Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat.
Joustra SD, Meijer OC, Heinen CA, Mol IM, Laghmani el H, Sengers RM, Carreno G, van Trotsenburg AS, Biermasz NR, Bernard DJ, Wit JM, Oostdijk W, van Pelt AM, Hamer G, Wagenaar GT
The Journal of endocrinology226(3)181-91(2015 Sep)

Modulation of activin signal transduction by inhibin B and inhibin-binding protein (INhBP).
Chapman SC, Woodruff TK
Molecular endocrinology (Baltimore, Md.)15(4)668-79(2001 Apr)

Inhibin binding protein in rats: alternative transcripts and regulation in the pituitary across the estrous cycle.
Bernard DJ, Woodruff TK
Molecular endocrinology (Baltimore, Md.)15(4)654-67(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Contrary to earlier predictions, neither the murine nor rat IGSF1-2 is secreted from heterologous or homologous cells. In addition, neither protein associates with the plasma membrane. Rather, IGSF1-2 appears to be retained in the endoplasmic reticulum.
Title: The short mRNA isoform of the immunoglobulin superfamily, member 1 gene encodes an intracellular glycoprotein.

High levels of Igsf1 in brain, pituitary and fetal liver. Also expressed in pancreas, heart, skeletal muscle, adrenal gland and testis.
Title: Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat.

The following Igsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Igsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa45953	XM_008773564.2 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa03961	NM_175763.2 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$972.30 $1389.00
ORa40244	XM_008773563.1 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa40244	XM_006257557.2 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa40245	XM_006257558.1 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa40246	XM_006257559.1 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa40247	XM_006257560.1 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa40248	XM_006257561.1 Latest version!	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Igsf1 ( XM_008773564.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771786.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa45953
Clone ID Related Accession (Same CDS sequence)	XM_008773564.2
Accession Version	XM_008773564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT GTGAATCATT TGGCCTGGCA GAGGGTGCTG ACTCAGTGTC ATTTCTAACT 
GTGGTCTCTG GATTGGTTTC AGATAAGCCC CCCAAACCCT CCCTTTCAGC TTGGCCCAGT 
TCCGTGTTCA AAGTAGGAAA GACCATCACC CTTCAGTGCC GAGTATCTCA TCCAGTTCTT 
GAATTTTCTT TGGAATGGGA AGAAACAACA ACATTCCAAA AATTCTCAGT AGACGGAGAC 
TTCATCATCA CTAACATTGA AGGGCAAGGC ACGGGTACCT ACAGTTGCAG CTATCGTATT 
GAGTCACACG CTAACATCTG GTCACATCGC AGTGAGCCTC TCAAGTTGGT GGGGCCAGCA 
GTCACAGGCT TCCTCCCCTG GAATTCCATT CTGAATGAAG CTATCAGGGT GTCCTTAACG 
GTGCAGTTTC TTTCCTTGCT GTTGCTAGTG CTGTGGCTAC AATGGAAGTG TCGGAGACTG 
AGACTCAGAG AAGCCTGGTT GTTGGGAACA GCTCAAGGGG TTGCCATGCT CGTCATACTC 
ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT GGGGCATTGA CAGAGGAGAT TGAAATCATC 
ATGCCAACCC CTAAACCTGA ACTGTGGGCA GAGACGAACT TCCCTCAGGC CCCGTGGAAG 
AACGTAACCC TCTGGTGCAG AAGCCCTTCT GGCTCAACTA AGGAGTTTGT GCTACTAAAG 
GACGGGACTG GGTGGATTGC AACTCGCCCG GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC 
CTTGGCGCCC TGACCCAAAG CCACACCGGG AGTTACCATT GCCATTCATG GGAGGAGATG 
GCTGTGTCGG AGCCTAGTGA AGCACTTGAA CTAGTGGGGA CAGACATCCT TCCCAAACCT 
GTCATTTCGG CTTCCCTTCC AATCCGGGGC CAGGAACTGC AGATCCGATG TAAAGGATGG 
CTGGAAGGCT TGGGGTTTGC TCTGTATAAG ATGGGAGAGC AGGAACCTGT CCAGCAACTC 
GGTGCTGTTG GGAGAGAAGC CTTCTTTACA ATTCAAAGAA TGGAGGATAA AGAGGAGGGC 
AATTACAGCT GCCGCACACA TACTGAAAAG CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT 
CCCCTGGAGC TTGTCATAAA AGAACTGTAC CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC 
CCCGTGGTCA CTCCTGGTTC TAGAGTGACT TTCAATTGCT CCACCTCCCG GGAACACATG 
AGTTTTATTC TTTACAAGGA TGGCAATGAA ATTGCATCCA GTGACCCAGT CTGGGGAAAC 
CCAGGAACCA GCACAGCACA CTTTCTGATC ATTTCAGTGG GCATTGGTGA TGGAGGAAAC 
TATAGCTGCC GTTATTATGA CTTTTCCATC TGGTCTGAGC CCAGCGACCC CGTGGAGCTT 
ATTGTGACAG AATTCTACCC CAAACCCACT CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT 
CCTGGGAAGA ATGTGACCCT GCGCTGTCAA GGAATTTTCC AGGGCATGAG GTTTGCCCTC 
TTACAGGAGG GTGCTCATGC TCCCTTACAG TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC 
TTCCTCCTCC ACACTGTTGG AGCAGAGGAC TTTGGGAACT ACAGCTGTGT CTACTATGAG 
ACAACCATGT CCAACAGGGG CTCATATCTT AGTACCCCCC TTATGATCTG GGTGACTGAC 
ACATTCCCCA GACCATGGTT GTCTGCTGAG CCTAGTTCTG TGGTCACCAT GGGGCAGAAT 
GTTACTCTAT GGTGCCAAGG ACCAGTCCAT GGAGTTGGGT ACATTCTGCA CAAAGAAGGA 
GAAGCCACTT CAATGCAGCT TTGGGATTCC ACTAGCAATG AAGGGGCATT CCCTATCATC 
AATATATCTG GTGCTAGCAT AGGACGTTAT AGCTGCTGTT ACCACCCTGA CTGGATGAGC 
CCTATCAAGA TACAGCCTAG CAACACTCTG GAACTCATAG TCACTGGTTT ACTCCCTAAG 
CCCAGCCTGT TAGTCCAGCC TGGTCCCATG GTAGTCCCCG GAGAAAACAT GACTTTTCAA 
TGTCAAGGAG AACTGCCAGA TTCGACATTT GTCCTGTTGA AGGAGGGGAC ACAACAGCCC 
ATAGAACAAC AGAGGCCAAG TGGGTACAGA GCTGACTTCT GGATGCCAGT GGTGAGGGAT 
CAAGATTCTG GTGTTTACAG CTGTGTTTAT TATCTGGATT CTGCTCCCCT TGTGGCTTCT 
AATCACAGCA ACTCTCTAGA GATCTGGGTG ACCGATAAGC CCCCTAAACC CTCTCTGTCA 
GCGTGGCCCA GCACTGTGTT CAAACTAGGA AAGGATATCA CCCTTCAGTG CCGAGGACCC 
CTGCCAGGAG TTGAATTCGT GCTAGAACAC GATGGAGAAG AGGCCCCCCA ACAGTTCTCA 
GAGGATGGAG ATTTTGTTAT CGGCAACATG GAAGGGAAAG GCATTGGAAA CTACAGTTGC 
AGCTATCGGC TGCAGGCTTA CCCTGACATC TGGTCAGAGC CCAGCGACTC CCTGGAGCTG 
GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG TGCACTGTGG GTAACATTGT CCGAAGTACC 
CTGATTGTGG TGGTTGTGGT AGCCTTGGGG ATAGTGCTAG CCATAGAGTG GAAGAAGTGG 
CCTCGACTTC GGACTAGGGG CTCTGAGACA GATGGGAGAG ACCAGACCAT AGTCCTTGAA 
GAGTGTAACC AAGATGGAGA ACCCGGCACT GCCACCAATT CTCCTTCATC TGCTTTGCAG 
GGTGTCTCAG TGGAACAAAC AGTCCCAATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771786.1
CDS	335..3187
Translation	MGSCESFGLAEGADSVSFLTVVSGLVSDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_008773564.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773564.2

ORF Insert Sequence:

ATGGGGAGCT GTGAATCATT TGGCCTGGCA GAGGGTGCTG ACTCAGTGTC ATTTCTAACT 
GTGGTCTCTG GATTGGTTTC AGATAAGCCC CCCAAACCCT CCCTTTCAGC TTGGCCCAGT 
TCCGTGTTCA AAGTAGGAAA GACCATCACC CTTCAGTGCC GAGTATCTCA TCCAGTTCTT 
GAATTTTCTT TGGAATGGGA AGAAACAACA ACATTCCAAA AATTCTCAGT AGACGGAGAC 
TTCATCATCA CTAACATTGA AGGGCAAGGC ACGGGTACCT ACAGTTGCAG CTATCGTATT 
GAGTCACACG CTAACATCTG GTCACATCGC AGTGAGCCTC TCAAGTTGGT GGGGCCAGCA 
GTCACAGGCT TCCTCCCCTG GAATTCCATT CTGAATGAAG CTATCAGGGT GTCCTTAACG 
GTGCAGTTTC TTTCCTTGCT GTTGCTAGTG CTGTGGCTAC AATGGAAGTG TCGGAGACTG 
AGACTCAGAG AAGCCTGGTT GTTGGGAACA GCTCAAGGGG TTGCCATGCT CGTCATACTC 
ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT GGGGCATTGA CAGAGGAGAT TGAAATCATC 
ATGCCAACCC CTAAACCTGA ACTGTGGGCA GAGACGAACT TCCCTCAGGC CCCGTGGAAG 
AACGTAACCC TCTGGTGCAG AAGCCCTTCT GGCTCAACTA AGGAGTTTGT GCTACTAAAG 
GACGGGACTG GGTGGATTGC AACTCGCCCG GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC 
CTTGGCGCCC TGACCCAAAG CCACACCGGG AGTTACCATT GCCATTCATG GGAGGAGATG 
GCTGTGTCGG AGCCTAGTGA AGCACTTGAA CTAGTGGGGA CAGACATCCT TCCCAAACCT 
GTCATTTCGG CTTCCCTTCC AATCCGGGGC CAGGAACTGC AGATCCGATG TAAAGGATGG 
CTGGAAGGCT TGGGGTTTGC TCTGTATAAG ATGGGAGAGC AGGAACCTGT CCAGCAACTC 
GGTGCTGTTG GGAGAGAAGC CTTCTTTACA ATTCAAAGAA TGGAGGATAA AGAGGAGGGC 
AATTACAGCT GCCGCACACA TACTGAAAAG CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT 
CCCCTGGAGC TTGTCATAAA AGAACTGTAC CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC 
CCCGTGGTCA CTCCTGGTTC TAGAGTGACT TTCAATTGCT CCACCTCCCG GGAACACATG 
AGTTTTATTC TTTACAAGGA TGGCAATGAA ATTGCATCCA GTGACCCAGT CTGGGGAAAC 
CCAGGAACCA GCACAGCACA CTTTCTGATC ATTTCAGTGG GCATTGGTGA TGGAGGAAAC 
TATAGCTGCC GTTATTATGA CTTTTCCATC TGGTCTGAGC CCAGCGACCC CGTGGAGCTT 
ATTGTGACAG AATTCTACCC CAAACCCACT CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT 
CCTGGGAAGA ATGTGACCCT GCGCTGTCAA GGAATTTTCC AGGGCATGAG GTTTGCCCTC 
TTACAGGAGG GTGCTCATGC TCCCTTACAG TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC 
TTCCTCCTCC ACACTGTTGG AGCAGAGGAC TTTGGGAACT ACAGCTGTGT CTACTATGAG 
ACAACCATGT CCAACAGGGG CTCATATCTT AGTACCCCCC TTATGATCTG GGTGACTGAC 
ACATTCCCCA GACCATGGTT GTCTGCTGAG CCTAGTTCTG TGGTCACCAT GGGGCAGAAT 
GTTACTCTAT GGTGCCAAGG ACCAGTCCAT GGAGTTGGGT ACATTCTGCA CAAAGAAGGA 
GAAGCCACTT CAATGCAGCT TTGGGATTCC ACTAGCAATG AAGGGGCATT CCCTATCATC 
AATATATCTG GTGCTAGCAT AGGACGTTAT AGCTGCTGTT ACCACCCTGA CTGGATGAGC 
CCTATCAAGA TACAGCCTAG CAACACTCTG GAACTCATAG TCACTGGTTT ACTCCCTAAG 
CCCAGCCTGT TAGTCCAGCC TGGTCCCATG GTAGTCCCCG GAGAAAACAT GACTTTTCAA 
TGTCAAGGAG AACTGCCAGA TTCGACATTT GTCCTGTTGA AGGAGGGGAC ACAACAGCCC 
ATAGAACAAC AGAGGCCAAG TGGGTACAGA GCTGACTTCT GGATGCCAGT GGTGAGGGAT 
CAAGATTCTG GTGTTTACAG CTGTGTTTAT TATCTGGATT CTGCTCCCCT TGTGGCTTCT 
AATCACAGCA ACTCTCTAGA GATCTGGGTG ACCGATAAGC CCCCTAAACC CTCTCTGTCA 
GCGTGGCCCA GCACTGTGTT CAAACTAGGA AAGGATATCA CCCTTCAGTG CCGAGGACCC 
CTGCCAGGAG TTGAATTCGT GCTAGAACAC GATGGAGAAG AGGCCCCCCA ACAGTTCTCA 
GAGGATGGAG ATTTTGTTAT CGGCAACATG GAAGGGAAAG GCATTGGAAA CTACAGTTGC 
AGCTATCGGC TGCAGGCTTA CCCTGACATC TGGTCAGAGC CCAGCGACTC CCTGGAGCTG 
GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG TGCACTGTGG GTAACATTGT CCGAAGTACC 
CTGATTGTGG TGGTTGTGGT AGCCTTGGGG ATAGTGCTAG CCATAGAGTG GAAGAAGTGG 
CCTCGACTTC GGACTAGGGG CTCTGAGACA GATGGGAGAG ACCAGACCAT AGTCCTTGAA 
GAGTGTAACC AAGATGGAGA ACCCGGCACT GCCACCAATT CTCCTTCATC TGCTTTGCAG 
GGTGTCTCAG TGGAACAAAC AGTCCCAATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( NM_175763.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_786939.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), mRNA.

Price & Availability↑

CloneID	ORa03961
Clone ID Related Accession (Same CDS sequence)	NM_175763.2
Accession Version	NM_175763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF322216.1. On Jun 3, 2003 this sequence version replaced NM_175763.1. ##Evidence-Data-START## Transcript exon combination :: AF322216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGC TTGGCCCAGT TCCGTGTTCA AAGTAGGAAA GACCATCACC 
CTTCAGTGCC GAGTATCTCA TCCAGTTCTT GAATTTTCTT TGGAATGGGA AGAAACAACA 
ACATTCCAAA AATTCTCAGT AGACGGAGAC TTCATCATCA CTAACATTGA AGGGCAAGGC 
ACGGGTACCT ACAGTTGCAG CTATCGTATT GAGTCACACG CTAACATCTG GTCACATCGC 
AGTGAGCCTC TCAAGTTGGT GGGGCCAGCA GTCACAGGCT TCCTCCCCTG GAATTCCATT 
CTGAATGAAG CTATCAGGGT GTCCTTAACG GTGCAGTTTC TTTCCTTGCT GTTGCTAGTG 
CTGTGGCTAC AATGGAAGTG TCGGAGACTG AGACTCAGAG AAGCCTGGTT GTTGGGAACA 
GCTCAAGGGG TTGCCATGCT CGTCATACTC ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT 
GGGGCATTGA CAGAGGAGAT TGAAATCATC ATGCCAACCC CTAAACCTGA ACTGTGGGCA 
GAGACGAACT TCCCTCAGGC CCCGTGGAAG AACGTAACCC TCTGGTGCAG AAGCCCTTCT 
GGCTCAACTA AGGAGTTTGT GCTACTAAAG GACGGGACTG GGTGGATTGC AACTCGCCCG 
GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC CTTGGCGCCC TGACCCAAAG CCACACCGGG 
AGTTACCATT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCTAGTGA AGCACTTGAA 
CTAGTGGGGA CAGACATCCT TCCCAAACCT GTCATTTCGG CTTCCCTTCC AATCCGGGGC 
CAGGAACTGC AGATCCGATG TAAAGGATGG CTGGAAGGCT TGGGGTTTGC TCTGTATAAG 
ATGGGAGAGC AGGAACCTGT CCAGCAACTC GGTGCTGTTG GGAGAGAAGC CTTCTTTACA 
ATTCAAAGAA TGGAGGATAA AGAGGAGGGC AATTACAGCT GCCGCACACA TACTGAAAAG 
CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT CCCCTGGAGC TTGTCATAAA AGAACTGTAC 
CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC CCCGTGGTCA CTCCTGGTTC TAGAGTGACT 
TTCAATTGCT CCACCTCCCG GGAACACATG AGTTTTATTC TTTACAAGGA TGGCAATGAA 
ATTGCATCCA GTGACCCAGT CTGGGGAAAC CCAGGAACCA GCACAGCACA CTTTCTGATC 
ATTTCAGTGG GCATTGGTGA TGGAGGAAAC TATAGCTGCC GTTATTATGA CTTTTCCATC 
TGGTCTGAGC CCAGCGACCC CGTGGAGCTT ATTGTGACAG AATTCTACCC CAAACCCACT 
CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT CCTGGGAAGA ATGTGACCCT GCGCTGTCAA 
GGAATTTTCC AGGGCATGAG GTTTGCCCTC TTACAGGAGG GTGCTCATGC TCCCTTACAG 
TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC TTCCTCCTCC ACACTGTTGG AGCAGAGGAC 
TTTGGGAACT ACAGCTGTGT CTACTATGAG ACAACCATGT CCAACAGGGG CTCATATCTT 
AGTACCCCCC TTATGATCTG GGTGACTGAC ACATTCCCCA GACCATGGTT GTCTGCTGAG 
CCTAGTTCTG TGGTCACCAT GGGGCAGAAT GTTACTCTAT GGTGCCAAGG ACCAGTCCAT 
GGAGTTGGGT ACATTCTGCA CAAAGAAGGA GAAGCCACTT CAATGCAGCT TTGGGATTCC 
ACTAGCAATG AAGGGGCATT CCCTATCATC AATATATCTG GTGCTAGCAT AGGACGTTAT 
AGCTGCTGTT ACCACCCTGA CTGGATGAGC CCTATCAAGA TACAGCCTAG CAACACTCTG 
GAACTCATAG TCACTGGTTT ACTCCCTAAG CCCAGCCTGT TAGTCCAGCC TGGTCCCATG 
GTAGTCCCCG GAGAAAACAT GACTTTTCAA TGTCAAGGAG AACTGCCAGA TTCGACATTT 
GTCCTGTTGA AGGAGGGGAC ACAACAGCCC ATAGAACAAC AGAGGCCAAG TGGGTACAGA 
GCTGACTTCT GGATGCCAGT GGTGAGGGAT CAAGATTCTG GTGTTTACAG CTGTGTTTAT 
TATCTGGATT CTGCTCCCCT TGTGGCTTCT AATCACAGCA ACTCTCTAGA GATCTGGGTG 
ACCGATAAGC CCCCTAAACC CTCTCTGTCA GCGTGGCCCA GCACTGTGTT CAAACTAGGA 
AAGGATATCA CCCTTCAGTG CCGAGGACCC CTGCCAGGAG TTGAATTCGT GCTAGAACAC 
GATGGAGAAG AGGCCCCCCA ACAGTTCTCA GAGGATGGAG ATTTTGTTAT CGGCAACATG 
GAAGGGAAAG GCATTGGAAA CTACAGTTGC AGCTATCGGC TGCAGGCTTA CCCTGACATC 
TGGTCAGAGC CCAGCGACTC CCTGGAGCTG GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG 
TGCACTGTGG GTAACATTGT CCGAAGTACC CTGATTGTGG TGGTTGTGGT AGCCTTGGGG 
ATAGTGCTAG CCATAGAGTG GAAGAAGTGG CCTCGACTTC GGACTAGGGG CTCTGAGACA 
GATGGGAGAG ACCAGACCAT AGTCCTTGAA GAGTGTAACC AAGATGGAGA ACCCGGCACT 
GCCACCAATT CTCCTTCATC TGCTTTGCAG GGTGTCTCAG TGGAACAAAC AGTCCCAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786939.1
CDS	172..4134
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	NM_175763.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175763.2

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGC TTGGCCCAGT TCCGTGTTCA AAGTAGGAAA GACCATCACC 
CTTCAGTGCC GAGTATCTCA TCCAGTTCTT GAATTTTCTT TGGAATGGGA AGAAACAACA 
ACATTCCAAA AATTCTCAGT AGACGGAGAC TTCATCATCA CTAACATTGA AGGGCAAGGC 
ACGGGTACCT ACAGTTGCAG CTATCGTATT GAGTCACACG CTAACATCTG GTCACATCGC 
AGTGAGCCTC TCAAGTTGGT GGGGCCAGCA GTCACAGGCT TCCTCCCCTG GAATTCCATT 
CTGAATGAAG CTATCAGGGT GTCCTTAACG GTGCAGTTTC TTTCCTTGCT GTTGCTAGTG 
CTGTGGCTAC AATGGAAGTG TCGGAGACTG AGACTCAGAG AAGCCTGGTT GTTGGGAACA 
GCTCAAGGGG TTGCCATGCT CGTCATACTC ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT 
GGGGCATTGA CAGAGGAGAT TGAAATCATC ATGCCAACCC CTAAACCTGA ACTGTGGGCA 
GAGACGAACT TCCCTCAGGC CCCGTGGAAG AACGTAACCC TCTGGTGCAG AAGCCCTTCT 
GGCTCAACTA AGGAGTTTGT GCTACTAAAG GACGGGACTG GGTGGATTGC AACTCGCCCG 
GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC CTTGGCGCCC TGACCCAAAG CCACACCGGG 
AGTTACCATT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCTAGTGA AGCACTTGAA 
CTAGTGGGGA CAGACATCCT TCCCAAACCT GTCATTTCGG CTTCCCTTCC AATCCGGGGC 
CAGGAACTGC AGATCCGATG TAAAGGATGG CTGGAAGGCT TGGGGTTTGC TCTGTATAAG 
ATGGGAGAGC AGGAACCTGT CCAGCAACTC GGTGCTGTTG GGAGAGAAGC CTTCTTTACA 
ATTCAAAGAA TGGAGGATAA AGAGGAGGGC AATTACAGCT GCCGCACACA TACTGAAAAG 
CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT CCCCTGGAGC TTGTCATAAA AGAACTGTAC 
CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC CCCGTGGTCA CTCCTGGTTC TAGAGTGACT 
TTCAATTGCT CCACCTCCCG GGAACACATG AGTTTTATTC TTTACAAGGA TGGCAATGAA 
ATTGCATCCA GTGACCCAGT CTGGGGAAAC CCAGGAACCA GCACAGCACA CTTTCTGATC 
ATTTCAGTGG GCATTGGTGA TGGAGGAAAC TATAGCTGCC GTTATTATGA CTTTTCCATC 
TGGTCTGAGC CCAGCGACCC CGTGGAGCTT ATTGTGACAG AATTCTACCC CAAACCCACT 
CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT CCTGGGAAGA ATGTGACCCT GCGCTGTCAA 
GGAATTTTCC AGGGCATGAG GTTTGCCCTC TTACAGGAGG GTGCTCATGC TCCCTTACAG 
TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC TTCCTCCTCC ACACTGTTGG AGCAGAGGAC 
TTTGGGAACT ACAGCTGTGT CTACTATGAG ACAACCATGT CCAACAGGGG CTCATATCTT 
AGTACCCCCC TTATGATCTG GGTGACTGAC ACATTCCCCA GACCATGGTT GTCTGCTGAG 
CCTAGTTCTG TGGTCACCAT GGGGCAGAAT GTTACTCTAT GGTGCCAAGG ACCAGTCCAT 
GGAGTTGGGT ACATTCTGCA CAAAGAAGGA GAAGCCACTT CAATGCAGCT TTGGGATTCC 
ACTAGCAATG AAGGGGCATT CCCTATCATC AATATATCTG GTGCTAGCAT AGGACGTTAT 
AGCTGCTGTT ACCACCCTGA CTGGATGAGC CCTATCAAGA TACAGCCTAG CAACACTCTG 
GAACTCATAG TCACTGGTTT ACTCCCTAAG CCCAGCCTGT TAGTCCAGCC TGGTCCCATG 
GTAGTCCCCG GAGAAAACAT GACTTTTCAA TGTCAAGGAG AACTGCCAGA TTCGACATTT 
GTCCTGTTGA AGGAGGGGAC ACAACAGCCC ATAGAACAAC AGAGGCCAAG TGGGTACAGA 
GCTGACTTCT GGATGCCAGT GGTGAGGGAT CAAGATTCTG GTGTTTACAG CTGTGTTTAT 
TATCTGGATT CTGCTCCCCT TGTGGCTTCT AATCACAGCA ACTCTCTAGA GATCTGGGTG 
ACCGATAAGC CCCCTAAACC CTCTCTGTCA GCGTGGCCCA GCACTGTGTT CAAACTAGGA 
AAGGATATCA CCCTTCAGTG CCGAGGACCC CTGCCAGGAG TTGAATTCGT GCTAGAACAC 
GATGGAGAAG AGGCCCCCCA ACAGTTCTCA GAGGATGGAG ATTTTGTTAT CGGCAACATG 
GAAGGGAAAG GCATTGGAAA CTACAGTTGC AGCTATCGGC TGCAGGCTTA CCCTGACATC 
TGGTCAGAGC CCAGCGACTC CCTGGAGCTG GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG 
TGCACTGTGG GTAACATTGT CCGAAGTACC CTGATTGTGG TGGTTGTGGT AGCCTTGGGG 
ATAGTGCTAG CCATAGAGTG GAAGAAGTGG CCTCGACTTC GGACTAGGGG CTCTGAGACA 
GATGGGAGAG ACCAGACCAT AGTCCTTGAA GAGTGTAACC AAGATGGAGA ACCCGGCACT 
GCCACCAATT CTCCTTCATC TGCTTTGCAG GGTGTCTCAG TGGAACAAAC AGTCCCAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_008773563.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771785.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X1, mRNA.

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CloneID	ORa40244
Clone ID Related Accession (Same CDS sequence)	XM_008773563.1 , XM_006257557.2
Accession Version	XM_008773563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGCCTGTTGC TCAAGAGTGC ACTGTGGGTA ACATTGTCCG AAGTACCCTG 
ATTGTGGTGG TTGTGGTAGC CTTGGGGATA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT 
CGACTTCGGA CTAGGGGCTC TGAGACAGAT GGGAGAGACC AGACCATAGT CCTTGAAGAG 
TGTAACCAAG ATGGAGAACC CGGCACTGCC ACCAATTCTC CTTCATCTGC TTTGCAGGGT 
GTCTCAGTGG AACAAACAGT CCCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771785.1
CDS	219..4208
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_008773563.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773563.1

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGCCTGTTGC TCAAGAGTGC ACTGTGGGTA ACATTGTCCG AAGTACCCTG 
ATTGTGGTGG TTGTGGTAGC CTTGGGGATA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT 
CGACTTCGGA CTAGGGGCTC TGAGACAGAT GGGAGAGACC AGACCATAGT CCTTGAAGAG 
TGTAACCAAG ATGGAGAACC CGGCACTGCC ACCAATTCTC CTTCATCTGC TTTGCAGGGT 
GTCTCAGTGG AACAAACAGT CCCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_006257557.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257619.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X2, mRNA.

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CloneID	ORa40244
Clone ID Related Accession (Same CDS sequence)	XM_008773563.1 , XM_006257557.2
Accession Version	XM_006257557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006257557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGCCTGTTGC TCAAGAGTGC ACTGTGGGTA ACATTGTCCG AAGTACCCTG 
ATTGTGGTGG TTGTGGTAGC CTTGGGGATA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT 
CGACTTCGGA CTAGGGGCTC TGAGACAGAT GGGAGAGACC AGACCATAGT CCTTGAAGAG 
TGTAACCAAG ATGGAGAACC CGGCACTGCC ACCAATTCTC CTTCATCTGC TTTGCAGGGT 
GTCTCAGTGG AACAAACAGT CCCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257619.1
CDS	266..4255
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_006257557.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257557.2

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGCCTGTTGC TCAAGAGTGC ACTGTGGGTA ACATTGTCCG AAGTACCCTG 
ATTGTGGTGG TTGTGGTAGC CTTGGGGATA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT 
CGACTTCGGA CTAGGGGCTC TGAGACAGAT GGGAGAGACC AGACCATAGT CCTTGAAGAG 
TGTAACCAAG ATGGAGAACC CGGCACTGCC ACCAATTCTC CTTCATCTGC TTTGCAGGGT 
GTCTCAGTGG AACAAACAGT CCCAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_006257558.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257620.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa40245
Clone ID Related Accession (Same CDS sequence)	XM_006257558.1
Accession Version	XM_006257558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG TGGAGTCTCA ACCAGAGCTA TGGATAGAGT CCAACTACCC CCAGGCCCCT 
TGGGAGAACA TCACACTTTG GTGCAAAAGC CCCTCTCGGG TATCCAGCAA GTTCCTGCTG 
CTGAAGGATA ACACACAGAT GACCTGGATT CATCCTCCTT ACAAGACCTT CCAAGTTTCA 
TTCTTCATAG GTGCCCTTAC TGAGTCCAAT ACAGGTCTTT ATCGATGCTG CTACTGGAAT 
GAGAAAGGCT GGTCGAAGCC CAGTAAAATT CTAGAGCTAG AAGCACCAGG CCAACTCCCT 
AAACCTATCT TCTGGATCCA GGCAGAGACC CCCCCTTTTC CTGGATGCAA TGTTAATATT 
CTCTGCCATG GATGGCTTCA GGATTTGGTA TTTATGCTGT TTAAAGAGGG ATACACAGAG 
CCCATAGATT ACCAAGTCCC AACTGGGACA ATGGCCATCT TCTCCATTGA CAACTTGGCA 
CCTGAGAATG AAGGCATTTA CATCTGCCGC ACCCATATCC AGATGCTCCC CACTCTGTGG 
TCAGAGCCCA GCAACCCCCT AAAGTTGGTG GTAGCAGGTC TCTACCCCAA ACCAACTCTG 
ACAGCCCATC CTGGGCCCAT CCTGGCACCA GGAGAAAGCC TGAGTCTCAG GTGCCAAGGG 
CCAATATATG GAATGACGTT TGCTTTAATG AGGTTGGAAG ACTTGAAGAA ACCATATTAC 
TACAAGAAGC CAATAAAAAA TGAGGCATAT TTCTACTTCC AGGCTCTGAA GACACAGGAT 
ACAGGGCACT ACCTCTGTTT TTACTATGAT GGGTCATACA GGGGCTCACT GCTTAGTGAT 
ATCCTGAAAA TCTGGGTGAC TGACACTTTT CCCAAGACCT GGCTACTTGT TCAGCCCAGC 
CCTGTGATTC AAATGGGTCA AAACGTGAGC CTGAGATGTG GAGGACTGAT GGATGGAGTG 
GGTCTTGCAC TCCATAAGAA GGGAGAAGAA AAGCCCCTTC AGTTTCTGGA TGCCACCAGC 
AACACTGGCA ACAATTCATT CTTCCTAAAG AATGTGACCT ACAGGGATGC TGGCATCTAT 
AGCTGCCACT ATTATCTTAC CTGGAAGACT TCCATTAAGA TGGCGACATA CAACACTGTG 
GAGCTGATTG TTGTAGATAA GCCCCCCAAA CCCTCCCTTT CAGCTTGGCC CAGTTCCGTG 
TTCAAAGTAG GAAAGACCAT CACCCTTCAG TGCCGAGTAT CTCATCCAGT TCTTGAATTT 
TCTTTGGAAT GGGAAGAAAC AACAACATTC CAAAAATTCT CAGTAGACGG AGACTTCATC 
ATCACTAACA TTGAAGGGCA AGGCACGGGT ACCTACAGTT GCAGCTATCG TATTGAGTCA 
CACGCTAACA TCTGGTCACA TCGCAGTGAG CCTCTCAAGT TGGTGGGGCC AGCAGTCACA 
GGCTTCCTCC CCTGGAATTC CATTCTGAAT GAAGCTATCA GGGTGTCCTT AACGGTGCAG 
TTTCTTTCCT TGCTGTTGCT AGTGCTGTGG CTACAATGGA AGTGTCGGAG ACTGAGACTC 
AGAGAAGCCT GGTTGTTGGG AACAGCTCAA GGGGTTGCCA TGCTCGTCAT ACTCATAGCT 
CTTCTTTGCT GTGGGCTGTG CAATGGGGCA TTGACAGAGG AGATTGAAAT CATCATGCCA 
ACCCCTAAAC CTGAACTGTG GGCAGAGACG AACTTCCCTC AGGCCCCGTG GAAGAACGTA 
ACCCTCTGGT GCAGAAGCCC TTCTGGCTCA ACTAAGGAGT TTGTGCTACT AAAGGACGGG 
ACTGGGTGGA TTGCAACTCG CCCGGCCTCA GAGCAGGTCC GGGCTGCCTT CCCCCTTGGC 
GCCCTGACCC AAAGCCACAC CGGGAGTTAC CATTGCCATT CATGGGAGGA GATGGCTGTG 
TCGGAGCCTA GTGAAGCACT TGAACTAGTG GGGACAGACA TCCTTCCCAA ACCTGTCATT 
TCGGCTTCCC TTCCAATCCG GGGCCAGGAA CTGCAGATCC GATGTAAAGG ATGGCTGGAA 
GGCTTGGGGT TTGCTCTGTA TAAGATGGGA GAGCAGGAAC CTGTCCAGCA ACTCGGTGCT 
GTTGGGAGAG AAGCCTTCTT TACAATTCAA AGAATGGAGG ATAAAGAGGA GGGCAATTAC 
AGCTGCCGCA CACATACTGA AAAGCAGCCC TTCAAGTGGT CTGAGCCCAG TGCTCCCCTG 
GAGCTTGTCA TAAAAGAACT GTACCCCAAG CCTTTCTTCA AGACATGGGC TAGCCCCGTG 
GTCACTCCTG GTTCTAGAGT GACTTTCAAT TGCTCCACCT CCCGGGAACA CATGAGTTTT 
ATTCTTTACA AGGATGGCAA TGAAATTGCA TCCAGTGACC CAGTCTGGGG AAACCCAGGA 
ACCAGCACAG CACACTTTCT GATCATTTCA GTGGGCATTG GTGATGGAGG AAACTATAGC 
TGCCGTTATT ATGACTTTTC CATCTGGTCT GAGCCCAGCG ACCCCGTGGA GCTTATTGTG 
ACAGAATTCT ACCCCAAACC CACTCTCCTG GCACAGCCAG GCCCTGTGGT GCTTCCTGGG 
AAGAATGTGA CCCTGCGCTG TCAAGGAATT TTCCAGGGCA TGAGGTTTGC CCTCTTACAG 
GAGGGTGCTC ATGCTCCCTT ACAGTTTCAG AGTGCCTCGG GGACCTCCGT TGACTTCCTC 
CTCCACACTG TTGGAGCAGA GGACTTTGGG AACTACAGCT GTGTCTACTA TGAGACAACC 
ATGTCCAACA GGGGCTCATA TCTTAGTACC CCCCTTATGA TCTGGGTGAC TGACACATTC 
CCCAGACCAT GGTTGTCTGC TGAGCCTAGT TCTGTGGTCA CCATGGGGCA GAATGTTACT 
CTATGGTGCC AAGGACCAGT CCATGGAGTT GGGTACATTC TGCACAAAGA AGGAGAAGCC 
ACTTCAATGC AGCTTTGGGA TTCCACTAGC AATGAAGGGG CATTCCCTAT CATCAATATA 
TCTGGTGCTA GCATAGGACG TTATAGCTGC TGTTACCACC CTGACTGGAT GAGCCCTATC 
AAGATACAGC CTAGCAACAC TCTGGAACTC ATAGTCACTG GTTTACTCCC TAAGCCCAGC 
CTGTTAGTCC AGCCTGGTCC CATGGTAGTC CCCGGAGAAA ACATGACTTT TCAATGTCAA 
GGAGAACTGC CAGATTCGAC ATTTGTCCTG TTGAAGGAGG GGACACAACA GCCCATAGAA 
CAACAGAGGC CAAGTGGGTA CAGAGCTGAC TTCTGGATGC CAGTGGTGAG GGATCAAGAT 
TCTGGTGTTT ACAGCTGTGT TTATTATCTG GATTCTGCTC CCCTTGTGGC TTCTAATCAC 
AGCAACTCTC TAGAGATCTG GGTGACCGAT AAGCCCCCTA AACCCTCTCT GTCAGCGTGG 
CCCAGCACTG TGTTCAAACT AGGAAAGGAT ATCACCCTTC AGTGCCGAGG ACCCCTGCCA 
GGAGTTGAAT TCGTGCTAGA ACACGATGGA GAAGAGGCCC CCCAACAGTT CTCAGAGGAT 
GGAGATTTTG TTATCGGCAA CATGGAAGGG AAAGGCATTG GAAACTACAG TTGCAGCTAT 
CGGCTGCAGG CTTACCCTGA CATCTGGTCA GAGCCCAGCG ACTCCCTGGA GCTGGTTGGA 
GCCGCAGGGC CTGTTGCTCA AGAGTGCACT GTGGGTAACA TTGTCCGAAG TACCCTGATT 
GTGGTGGTTG TGGTAGCCTT GGGGATAGTG CTAGCCATAG AGTGGAAGAA GTGGCCTCGA 
CTTCGGACTA GGGGCTCTGA GACAGATGGG AGAGACCAGA CCATAGTCCT TGAAGAGTGT 
AACCAAGATG GAGAACCCGG CACTGCCACC AATTCTCCTT CATCTGCTTT GCAGGGTGTC 
TCAGTGGAAC AAACAGTCCC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257620.1
CDS	219..4205
Translation	MMLRTFTLLLLCIWLNRGMTSMAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_006257558.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257558.1

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG TGGAGTCTCA ACCAGAGCTA TGGATAGAGT CCAACTACCC CCAGGCCCCT 
TGGGAGAACA TCACACTTTG GTGCAAAAGC CCCTCTCGGG TATCCAGCAA GTTCCTGCTG 
CTGAAGGATA ACACACAGAT GACCTGGATT CATCCTCCTT ACAAGACCTT CCAAGTTTCA 
TTCTTCATAG GTGCCCTTAC TGAGTCCAAT ACAGGTCTTT ATCGATGCTG CTACTGGAAT 
GAGAAAGGCT GGTCGAAGCC CAGTAAAATT CTAGAGCTAG AAGCACCAGG CCAACTCCCT 
AAACCTATCT TCTGGATCCA GGCAGAGACC CCCCCTTTTC CTGGATGCAA TGTTAATATT 
CTCTGCCATG GATGGCTTCA GGATTTGGTA TTTATGCTGT TTAAAGAGGG ATACACAGAG 
CCCATAGATT ACCAAGTCCC AACTGGGACA ATGGCCATCT TCTCCATTGA CAACTTGGCA 
CCTGAGAATG AAGGCATTTA CATCTGCCGC ACCCATATCC AGATGCTCCC CACTCTGTGG 
TCAGAGCCCA GCAACCCCCT AAAGTTGGTG GTAGCAGGTC TCTACCCCAA ACCAACTCTG 
ACAGCCCATC CTGGGCCCAT CCTGGCACCA GGAGAAAGCC TGAGTCTCAG GTGCCAAGGG 
CCAATATATG GAATGACGTT TGCTTTAATG AGGTTGGAAG ACTTGAAGAA ACCATATTAC 
TACAAGAAGC CAATAAAAAA TGAGGCATAT TTCTACTTCC AGGCTCTGAA GACACAGGAT 
ACAGGGCACT ACCTCTGTTT TTACTATGAT GGGTCATACA GGGGCTCACT GCTTAGTGAT 
ATCCTGAAAA TCTGGGTGAC TGACACTTTT CCCAAGACCT GGCTACTTGT TCAGCCCAGC 
CCTGTGATTC AAATGGGTCA AAACGTGAGC CTGAGATGTG GAGGACTGAT GGATGGAGTG 
GGTCTTGCAC TCCATAAGAA GGGAGAAGAA AAGCCCCTTC AGTTTCTGGA TGCCACCAGC 
AACACTGGCA ACAATTCATT CTTCCTAAAG AATGTGACCT ACAGGGATGC TGGCATCTAT 
AGCTGCCACT ATTATCTTAC CTGGAAGACT TCCATTAAGA TGGCGACATA CAACACTGTG 
GAGCTGATTG TTGTAGATAA GCCCCCCAAA CCCTCCCTTT CAGCTTGGCC CAGTTCCGTG 
TTCAAAGTAG GAAAGACCAT CACCCTTCAG TGCCGAGTAT CTCATCCAGT TCTTGAATTT 
TCTTTGGAAT GGGAAGAAAC AACAACATTC CAAAAATTCT CAGTAGACGG AGACTTCATC 
ATCACTAACA TTGAAGGGCA AGGCACGGGT ACCTACAGTT GCAGCTATCG TATTGAGTCA 
CACGCTAACA TCTGGTCACA TCGCAGTGAG CCTCTCAAGT TGGTGGGGCC AGCAGTCACA 
GGCTTCCTCC CCTGGAATTC CATTCTGAAT GAAGCTATCA GGGTGTCCTT AACGGTGCAG 
TTTCTTTCCT TGCTGTTGCT AGTGCTGTGG CTACAATGGA AGTGTCGGAG ACTGAGACTC 
AGAGAAGCCT GGTTGTTGGG AACAGCTCAA GGGGTTGCCA TGCTCGTCAT ACTCATAGCT 
CTTCTTTGCT GTGGGCTGTG CAATGGGGCA TTGACAGAGG AGATTGAAAT CATCATGCCA 
ACCCCTAAAC CTGAACTGTG GGCAGAGACG AACTTCCCTC AGGCCCCGTG GAAGAACGTA 
ACCCTCTGGT GCAGAAGCCC TTCTGGCTCA ACTAAGGAGT TTGTGCTACT AAAGGACGGG 
ACTGGGTGGA TTGCAACTCG CCCGGCCTCA GAGCAGGTCC GGGCTGCCTT CCCCCTTGGC 
GCCCTGACCC AAAGCCACAC CGGGAGTTAC CATTGCCATT CATGGGAGGA GATGGCTGTG 
TCGGAGCCTA GTGAAGCACT TGAACTAGTG GGGACAGACA TCCTTCCCAA ACCTGTCATT 
TCGGCTTCCC TTCCAATCCG GGGCCAGGAA CTGCAGATCC GATGTAAAGG ATGGCTGGAA 
GGCTTGGGGT TTGCTCTGTA TAAGATGGGA GAGCAGGAAC CTGTCCAGCA ACTCGGTGCT 
GTTGGGAGAG AAGCCTTCTT TACAATTCAA AGAATGGAGG ATAAAGAGGA GGGCAATTAC 
AGCTGCCGCA CACATACTGA AAAGCAGCCC TTCAAGTGGT CTGAGCCCAG TGCTCCCCTG 
GAGCTTGTCA TAAAAGAACT GTACCCCAAG CCTTTCTTCA AGACATGGGC TAGCCCCGTG 
GTCACTCCTG GTTCTAGAGT GACTTTCAAT TGCTCCACCT CCCGGGAACA CATGAGTTTT 
ATTCTTTACA AGGATGGCAA TGAAATTGCA TCCAGTGACC CAGTCTGGGG AAACCCAGGA 
ACCAGCACAG CACACTTTCT GATCATTTCA GTGGGCATTG GTGATGGAGG AAACTATAGC 
TGCCGTTATT ATGACTTTTC CATCTGGTCT GAGCCCAGCG ACCCCGTGGA GCTTATTGTG 
ACAGAATTCT ACCCCAAACC CACTCTCCTG GCACAGCCAG GCCCTGTGGT GCTTCCTGGG 
AAGAATGTGA CCCTGCGCTG TCAAGGAATT TTCCAGGGCA TGAGGTTTGC CCTCTTACAG 
GAGGGTGCTC ATGCTCCCTT ACAGTTTCAG AGTGCCTCGG GGACCTCCGT TGACTTCCTC 
CTCCACACTG TTGGAGCAGA GGACTTTGGG AACTACAGCT GTGTCTACTA TGAGACAACC 
ATGTCCAACA GGGGCTCATA TCTTAGTACC CCCCTTATGA TCTGGGTGAC TGACACATTC 
CCCAGACCAT GGTTGTCTGC TGAGCCTAGT TCTGTGGTCA CCATGGGGCA GAATGTTACT 
CTATGGTGCC AAGGACCAGT CCATGGAGTT GGGTACATTC TGCACAAAGA AGGAGAAGCC 
ACTTCAATGC AGCTTTGGGA TTCCACTAGC AATGAAGGGG CATTCCCTAT CATCAATATA 
TCTGGTGCTA GCATAGGACG TTATAGCTGC TGTTACCACC CTGACTGGAT GAGCCCTATC 
AAGATACAGC CTAGCAACAC TCTGGAACTC ATAGTCACTG GTTTACTCCC TAAGCCCAGC 
CTGTTAGTCC AGCCTGGTCC CATGGTAGTC CCCGGAGAAA ACATGACTTT TCAATGTCAA 
GGAGAACTGC CAGATTCGAC ATTTGTCCTG TTGAAGGAGG GGACACAACA GCCCATAGAA 
CAACAGAGGC CAAGTGGGTA CAGAGCTGAC TTCTGGATGC CAGTGGTGAG GGATCAAGAT 
TCTGGTGTTT ACAGCTGTGT TTATTATCTG GATTCTGCTC CCCTTGTGGC TTCTAATCAC 
AGCAACTCTC TAGAGATCTG GGTGACCGAT AAGCCCCCTA AACCCTCTCT GTCAGCGTGG 
CCCAGCACTG TGTTCAAACT AGGAAAGGAT ATCACCCTTC AGTGCCGAGG ACCCCTGCCA 
GGAGTTGAAT TCGTGCTAGA ACACGATGGA GAAGAGGCCC CCCAACAGTT CTCAGAGGAT 
GGAGATTTTG TTATCGGCAA CATGGAAGGG AAAGGCATTG GAAACTACAG TTGCAGCTAT 
CGGCTGCAGG CTTACCCTGA CATCTGGTCA GAGCCCAGCG ACTCCCTGGA GCTGGTTGGA 
GCCGCAGGGC CTGTTGCTCA AGAGTGCACT GTGGGTAACA TTGTCCGAAG TACCCTGATT 
GTGGTGGTTG TGGTAGCCTT GGGGATAGTG CTAGCCATAG AGTGGAAGAA GTGGCCTCGA 
CTTCGGACTA GGGGCTCTGA GACAGATGGG AGAGACCAGA CCATAGTCCT TGAAGAGTGT 
AACCAAGATG GAGAACCCGG CACTGCCACC AATTCTCCTT CATCTGCTTT GCAGGGTGTC 
TCAGTGGAAC AAACAGTCCC AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_006257559.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257621.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X4, mRNA.

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CloneID	ORa40246
Clone ID Related Accession (Same CDS sequence)	XM_006257559.1
Accession Version	XM_006257559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTG 
CTGTGGCTAC AATGGAAGTG TCGGAGACTG AGACTCAGAG AAGCCTGGTT GTTGGGAACA 
GCTCAAGGGG TTGCCATGCT CGTCATACTC ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT 
GGGGCATTGA CAGAGGAGAT TGAAATCATC ATGCCAACCC CTAAACCTGA ACTGTGGGCA 
GAGACGAACT TCCCTCAGGC CCCGTGGAAG AACGTAACCC TCTGGTGCAG AAGCCCTTCT 
GGCTCAACTA AGGAGTTTGT GCTACTAAAG GACGGGACTG GGTGGATTGC AACTCGCCCG 
GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC CTTGGCGCCC TGACCCAAAG CCACACCGGG 
AGTTACCATT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCTAGTGA AGCACTTGAA 
CTAGTGGGGA CAGACATCCT TCCCAAACCT GTCATTTCGG CTTCCCTTCC AATCCGGGGC 
CAGGAACTGC AGATCCGATG TAAAGGATGG CTGGAAGGCT TGGGGTTTGC TCTGTATAAG 
ATGGGAGAGC AGGAACCTGT CCAGCAACTC GGTGCTGTTG GGAGAGAAGC CTTCTTTACA 
ATTCAAAGAA TGGAGGATAA AGAGGAGGGC AATTACAGCT GCCGCACACA TACTGAAAAG 
CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT CCCCTGGAGC TTGTCATAAA AGAACTGTAC 
CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC CCCGTGGTCA CTCCTGGTTC TAGAGTGACT 
TTCAATTGCT CCACCTCCCG GGAACACATG AGTTTTATTC TTTACAAGGA TGGCAATGAA 
ATTGCATCCA GTGACCCAGT CTGGGGAAAC CCAGGAACCA GCACAGCACA CTTTCTGATC 
ATTTCAGTGG GCATTGGTGA TGGAGGAAAC TATAGCTGCC GTTATTATGA CTTTTCCATC 
TGGTCTGAGC CCAGCGACCC CGTGGAGCTT ATTGTGACAG AATTCTACCC CAAACCCACT 
CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT CCTGGGAAGA ATGTGACCCT GCGCTGTCAA 
GGAATTTTCC AGGGCATGAG GTTTGCCCTC TTACAGGAGG GTGCTCATGC TCCCTTACAG 
TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC TTCCTCCTCC ACACTGTTGG AGCAGAGGAC 
TTTGGGAACT ACAGCTGTGT CTACTATGAG ACAACCATGT CCAACAGGGG CTCATATCTT 
AGTACCCCCC TTATGATCTG GGTGACTGAC ACATTCCCCA GACCATGGTT GTCTGCTGAG 
CCTAGTTCTG TGGTCACCAT GGGGCAGAAT GTTACTCTAT GGTGCCAAGG ACCAGTCCAT 
GGAGTTGGGT ACATTCTGCA CAAAGAAGGA GAAGCCACTT CAATGCAGCT TTGGGATTCC 
ACTAGCAATG AAGGGGCATT CCCTATCATC AATATATCTG GTGCTAGCAT AGGACGTTAT 
AGCTGCTGTT ACCACCCTGA CTGGATGAGC CCTATCAAGA TACAGCCTAG CAACACTCTG 
GAACTCATAG TCACTGGTTT ACTCCCTAAG CCCAGCCTGT TAGTCCAGCC TGGTCCCATG 
GTAGTCCCCG GAGAAAACAT GACTTTTCAA TGTCAAGGAG AACTGCCAGA TTCGACATTT 
GTCCTGTTGA AGGAGGGGAC ACAACAGCCC ATAGAACAAC AGAGGCCAAG TGGGTACAGA 
GCTGACTTCT GGATGCCAGT GGTGAGGGAT CAAGATTCTG GTGTTTACAG CTGTGTTTAT 
TATCTGGATT CTGCTCCCCT TGTGGCTTCT AATCACAGCA ACTCTCTAGA GATCTGGGTG 
ACCGATAAGC CCCCTAAACC CTCTCTGTCA GCGTGGCCCA GCACTGTGTT CAAACTAGGA 
AAGGATATCA CCCTTCAGTG CCGAGGACCC CTGCCAGGAG TTGAATTCGT GCTAGAACAC 
GATGGAGAAG AGGCCCCCCA ACAGTTCTCA GAGGATGGAG ATTTTGTTAT CGGCAACATG 
GAAGGGAAAG GCATTGGAAA CTACAGTTGC AGCTATCGGC TGCAGGCTTA CCCTGACATC 
TGGTCAGAGC CCAGCGACTC CCTGGAGCTG GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG 
TGCACTGTGG GTAACATTGT CCGAAGTACC CTGATTGTGG TGGTTGTGGT AGCCTTGGGG 
ATAGTGCTAG CCATAGAGTG GAAGAAGTGG CCTCGACTTC GGACTAGGGG CTCTGAGACA 
GATGGGAGAG ACCAGACCAT AGTCCTTGAA GAGTGTAACC AAGATGGAGA ACCCGGCACT 
GCCACCAATT CTCCTTCATC TGCTTTGCAG GGTGTCTCAG TGGAACAAAC AGTCCCAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257621.1
CDS	219..4121
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_006257559.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257559.1

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTG 
CTGTGGCTAC AATGGAAGTG TCGGAGACTG AGACTCAGAG AAGCCTGGTT GTTGGGAACA 
GCTCAAGGGG TTGCCATGCT CGTCATACTC ATAGCTCTTC TTTGCTGTGG GCTGTGCAAT 
GGGGCATTGA CAGAGGAGAT TGAAATCATC ATGCCAACCC CTAAACCTGA ACTGTGGGCA 
GAGACGAACT TCCCTCAGGC CCCGTGGAAG AACGTAACCC TCTGGTGCAG AAGCCCTTCT 
GGCTCAACTA AGGAGTTTGT GCTACTAAAG GACGGGACTG GGTGGATTGC AACTCGCCCG 
GCCTCAGAGC AGGTCCGGGC TGCCTTCCCC CTTGGCGCCC TGACCCAAAG CCACACCGGG 
AGTTACCATT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCTAGTGA AGCACTTGAA 
CTAGTGGGGA CAGACATCCT TCCCAAACCT GTCATTTCGG CTTCCCTTCC AATCCGGGGC 
CAGGAACTGC AGATCCGATG TAAAGGATGG CTGGAAGGCT TGGGGTTTGC TCTGTATAAG 
ATGGGAGAGC AGGAACCTGT CCAGCAACTC GGTGCTGTTG GGAGAGAAGC CTTCTTTACA 
ATTCAAAGAA TGGAGGATAA AGAGGAGGGC AATTACAGCT GCCGCACACA TACTGAAAAG 
CAGCCCTTCA AGTGGTCTGA GCCCAGTGCT CCCCTGGAGC TTGTCATAAA AGAACTGTAC 
CCCAAGCCTT TCTTCAAGAC ATGGGCTAGC CCCGTGGTCA CTCCTGGTTC TAGAGTGACT 
TTCAATTGCT CCACCTCCCG GGAACACATG AGTTTTATTC TTTACAAGGA TGGCAATGAA 
ATTGCATCCA GTGACCCAGT CTGGGGAAAC CCAGGAACCA GCACAGCACA CTTTCTGATC 
ATTTCAGTGG GCATTGGTGA TGGAGGAAAC TATAGCTGCC GTTATTATGA CTTTTCCATC 
TGGTCTGAGC CCAGCGACCC CGTGGAGCTT ATTGTGACAG AATTCTACCC CAAACCCACT 
CTCCTGGCAC AGCCAGGCCC TGTGGTGCTT CCTGGGAAGA ATGTGACCCT GCGCTGTCAA 
GGAATTTTCC AGGGCATGAG GTTTGCCCTC TTACAGGAGG GTGCTCATGC TCCCTTACAG 
TTTCAGAGTG CCTCGGGGAC CTCCGTTGAC TTCCTCCTCC ACACTGTTGG AGCAGAGGAC 
TTTGGGAACT ACAGCTGTGT CTACTATGAG ACAACCATGT CCAACAGGGG CTCATATCTT 
AGTACCCCCC TTATGATCTG GGTGACTGAC ACATTCCCCA GACCATGGTT GTCTGCTGAG 
CCTAGTTCTG TGGTCACCAT GGGGCAGAAT GTTACTCTAT GGTGCCAAGG ACCAGTCCAT 
GGAGTTGGGT ACATTCTGCA CAAAGAAGGA GAAGCCACTT CAATGCAGCT TTGGGATTCC 
ACTAGCAATG AAGGGGCATT CCCTATCATC AATATATCTG GTGCTAGCAT AGGACGTTAT 
AGCTGCTGTT ACCACCCTGA CTGGATGAGC CCTATCAAGA TACAGCCTAG CAACACTCTG 
GAACTCATAG TCACTGGTTT ACTCCCTAAG CCCAGCCTGT TAGTCCAGCC TGGTCCCATG 
GTAGTCCCCG GAGAAAACAT GACTTTTCAA TGTCAAGGAG AACTGCCAGA TTCGACATTT 
GTCCTGTTGA AGGAGGGGAC ACAACAGCCC ATAGAACAAC AGAGGCCAAG TGGGTACAGA 
GCTGACTTCT GGATGCCAGT GGTGAGGGAT CAAGATTCTG GTGTTTACAG CTGTGTTTAT 
TATCTGGATT CTGCTCCCCT TGTGGCTTCT AATCACAGCA ACTCTCTAGA GATCTGGGTG 
ACCGATAAGC CCCCTAAACC CTCTCTGTCA GCGTGGCCCA GCACTGTGTT CAAACTAGGA 
AAGGATATCA CCCTTCAGTG CCGAGGACCC CTGCCAGGAG TTGAATTCGT GCTAGAACAC 
GATGGAGAAG AGGCCCCCCA ACAGTTCTCA GAGGATGGAG ATTTTGTTAT CGGCAACATG 
GAAGGGAAAG GCATTGGAAA CTACAGTTGC AGCTATCGGC TGCAGGCTTA CCCTGACATC 
TGGTCAGAGC CCAGCGACTC CCTGGAGCTG GTTGGAGCCG CAGGGCCTGT TGCTCAAGAG 
TGCACTGTGG GTAACATTGT CCGAAGTACC CTGATTGTGG TGGTTGTGGT AGCCTTGGGG 
ATAGTGCTAG CCATAGAGTG GAAGAAGTGG CCTCGACTTC GGACTAGGGG CTCTGAGACA 
GATGGGAGAG ACCAGACCAT AGTCCTTGAA GAGTGTAACC AAGATGGAGA ACCCGGCACT 
GCCACCAATT CTCCTTCATC TGCTTTGCAG GGTGTCTCAG TGGAACAAAC AGTCCCAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_006257560.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257622.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa40247
Clone ID Related Accession (Same CDS sequence)	XM_006257560.1
Accession Version	XM_006257560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGGCTCTGAG ACAGATGGGA GAGACCAGAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257622.1
CDS	219..3983
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPKPSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVYYLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGALRQMGETRP

Target ORF information:

RefSeq Version	XM_006257560.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257560.1

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGGTTTACT CCCTAAGCCC 
AGCCTGTTAG TCCAGCCTGG TCCCATGGTA GTCCCCGGAG AAAACATGAC TTTTCAATGT 
CAAGGAGAAC TGCCAGATTC GACATTTGTC CTGTTGAAGG AGGGGACACA ACAGCCCATA 
GAACAACAGA GGCCAAGTGG GTACAGAGCT GACTTCTGGA TGCCAGTGGT GAGGGATCAA 
GATTCTGGTG TTTACAGCTG TGTTTATTAT CTGGATTCTG CTCCCCTTGT GGCTTCTAAT 
CACAGCAACT CTCTAGAGAT CTGGGTGACC GATAAGCCCC CTAAACCCTC TCTGTCAGCG 
TGGCCCAGCA CTGTGTTCAA ACTAGGAAAG GATATCACCC TTCAGTGCCG AGGACCCCTG 
CCAGGAGTTG AATTCGTGCT AGAACACGAT GGAGAAGAGG CCCCCCAACA GTTCTCAGAG 
GATGGAGATT TTGTTATCGG CAACATGGAA GGGAAAGGCA TTGGAAACTA CAGTTGCAGC 
TATCGGCTGC AGGCTTACCC TGACATCTGG TCAGAGCCCA GCGACTCCCT GGAGCTGGTT 
GGAGCCGCAG GGGCTCTGAG ACAGATGGGA GAGACCAGAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igsf1 ( XM_006257561.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257623.1 Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa40248
Clone ID Related Accession (Same CDS sequence)	XM_006257561.1
Accession Version	XM_006257561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	immunoglobulin superfamily member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGATAAGCC CCCTAAACCC 
TCTCTGTCAG CGTGGCCCAG CACTGTGTTC AAACTAGGAA AGGATATCAC CCTTCAGTGC 
CGAGGACCCC TGCCAGGAGT TGAATTCGTG CTAGAACACG ATGGAGAAGA GGCCCCCCAA 
CAGTTCTCAG AGGATGGAGA TTTTGTTATC GGCAACATGG AAGGGAAAGG CATTGGAAAC 
TACAGTTGCA GCTATCGGCT GCAGGCTTAC CCTGACATCT GGTCAGAGCC CAGCGACTCC 
CTGGAGCTGG TTGGAGCCGC AGGGCCTGTT GCTCAAGAGT GCACTGTGGG TAACATTGTC 
CGAAGTACCC TGATTGTGGT GGTTGTGGTA GCCTTGGGGA TAGTGCTAGC CATAGAGTGG 
AAGAAGTGGC CTCGACTTCG GACTAGGGGC TCTGAGACAG ATGGGAGAGA CCAGACCATA 
GTCCTTGAAG AGTGTAACCA AGATGGAGAA CCCGGCACTG CCACCAATTC TCCTTCATCT 
GCTTTGCAGG GTGTCTCAGT GGAACAAACA GTCCCAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257623.1
CDS	219..3920
Translation	MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTWIHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVNILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKLVVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQDTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGEEKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVDKPPKPSLSAWPSSVFKVGKTITLQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPAVTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCNGALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYKMGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWASPVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVDFLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVHGVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALGIVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI

Target ORF information:

RefSeq Version	XM_006257561.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus immunoglobulin superfamily, member 1 (Igsf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257561.1

ORF Insert Sequence:

ATGATGCTTC GGACCTTCAC TCTTTTGCTC CTTTGCATTT GGCTCAATCG GGGTATGACT 
TCAATGGCAG CAGTGGAGTC TCAACCAGAG CTATGGATAG AGTCCAACTA CCCCCAGGCC 
CCTTGGGAGA ACATCACACT TTGGTGCAAA AGCCCCTCTC GGGTATCCAG CAAGTTCCTG 
CTGCTGAAGG ATAACACACA GATGACCTGG ATTCATCCTC CTTACAAGAC CTTCCAAGTT 
TCATTCTTCA TAGGTGCCCT TACTGAGTCC AATACAGGTC TTTATCGATG CTGCTACTGG 
AATGAGAAAG GCTGGTCGAA GCCCAGTAAA ATTCTAGAGC TAGAAGCACC AGGCCAACTC 
CCTAAACCTA TCTTCTGGAT CCAGGCAGAG ACCCCCCCTT TTCCTGGATG CAATGTTAAT 
ATTCTCTGCC ATGGATGGCT TCAGGATTTG GTATTTATGC TGTTTAAAGA GGGATACACA 
GAGCCCATAG ATTACCAAGT CCCAACTGGG ACAATGGCCA TCTTCTCCAT TGACAACTTG 
GCACCTGAGA ATGAAGGCAT TTACATCTGC CGCACCCATA TCCAGATGCT CCCCACTCTG 
TGGTCAGAGC CCAGCAACCC CCTAAAGTTG GTGGTAGCAG GTCTCTACCC CAAACCAACT 
CTGACAGCCC ATCCTGGGCC CATCCTGGCA CCAGGAGAAA GCCTGAGTCT CAGGTGCCAA 
GGGCCAATAT ATGGAATGAC GTTTGCTTTA ATGAGGTTGG AAGACTTGAA GAAACCATAT 
TACTACAAGA AGCCAATAAA AAATGAGGCA TATTTCTACT TCCAGGCTCT GAAGACACAG 
GATACAGGGC ACTACCTCTG TTTTTACTAT GATGGGTCAT ACAGGGGCTC ACTGCTTAGT 
GATATCCTGA AAATCTGGGT GACTGACACT TTTCCCAAGA CCTGGCTACT TGTTCAGCCC 
AGCCCTGTGA TTCAAATGGG TCAAAACGTG AGCCTGAGAT GTGGAGGACT GATGGATGGA 
GTGGGTCTTG CACTCCATAA GAAGGGAGAA GAAAAGCCCC TTCAGTTTCT GGATGCCACC 
AGCAACACTG GCAACAATTC ATTCTTCCTA AAGAATGTGA CCTACAGGGA TGCTGGCATC 
TATAGCTGCC ACTATTATCT TACCTGGAAG ACTTCCATTA AGATGGCGAC ATACAACACT 
GTGGAGCTGA TTGTTGTAGA TAAGCCCCCC AAACCCTCCC TTTCAGCTTG GCCCAGTTCC 
GTGTTCAAAG TAGGAAAGAC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTTCTTGAA 
TTTTCTTTGG AATGGGAAGA AACAACAACA TTCCAAAAAT TCTCAGTAGA CGGAGACTTC 
ATCATCACTA ACATTGAAGG GCAAGGCACG GGTACCTACA GTTGCAGCTA TCGTATTGAG 
TCACACGCTA ACATCTGGTC ACATCGCAGT GAGCCTCTCA AGTTGGTGGG GCCAGCAGTC 
ACAGGCTTCC TCCCCTGGAA TTCCATTCTG AATGAAGCTA TCAGGGTGTC CTTAACGGTG 
CAGTTTCTTT CCTTGCTGTT GCTAGTGCTG TGGCTACAAT GGAAGTGTCG GAGACTGAGA 
CTCAGAGAAG CCTGGTTGTT GGGAACAGCT CAAGGGGTTG CCATGCTCGT CATACTCATA 
GCTCTTCTTT GCTGTGGGCT GTGCAATGGG GCATTGACAG AGGAGATTGA AATCATCATG 
CCAACCCCTA AACCTGAACT GTGGGCAGAG ACGAACTTCC CTCAGGCCCC GTGGAAGAAC 
GTAACCCTCT GGTGCAGAAG CCCTTCTGGC TCAACTAAGG AGTTTGTGCT ACTAAAGGAC 
GGGACTGGGT GGATTGCAAC TCGCCCGGCC TCAGAGCAGG TCCGGGCTGC CTTCCCCCTT 
GGCGCCCTGA CCCAAAGCCA CACCGGGAGT TACCATTGCC ATTCATGGGA GGAGATGGCT 
GTGTCGGAGC CTAGTGAAGC ACTTGAACTA GTGGGGACAG ACATCCTTCC CAAACCTGTC 
ATTTCGGCTT CCCTTCCAAT CCGGGGCCAG GAACTGCAGA TCCGATGTAA AGGATGGCTG 
GAAGGCTTGG GGTTTGCTCT GTATAAGATG GGAGAGCAGG AACCTGTCCA GCAACTCGGT 
GCTGTTGGGA GAGAAGCCTT CTTTACAATT CAAAGAATGG AGGATAAAGA GGAGGGCAAT 
TACAGCTGCC GCACACATAC TGAAAAGCAG CCCTTCAAGT GGTCTGAGCC CAGTGCTCCC 
CTGGAGCTTG TCATAAAAGA ACTGTACCCC AAGCCTTTCT TCAAGACATG GGCTAGCCCC 
GTGGTCACTC CTGGTTCTAG AGTGACTTTC AATTGCTCCA CCTCCCGGGA ACACATGAGT 
TTTATTCTTT ACAAGGATGG CAATGAAATT GCATCCAGTG ACCCAGTCTG GGGAAACCCA 
GGAACCAGCA CAGCACACTT TCTGATCATT TCAGTGGGCA TTGGTGATGG AGGAAACTAT 
AGCTGCCGTT ATTATGACTT TTCCATCTGG TCTGAGCCCA GCGACCCCGT GGAGCTTATT 
GTGACAGAAT TCTACCCCAA ACCCACTCTC CTGGCACAGC CAGGCCCTGT GGTGCTTCCT 
GGGAAGAATG TGACCCTGCG CTGTCAAGGA ATTTTCCAGG GCATGAGGTT TGCCCTCTTA 
CAGGAGGGTG CTCATGCTCC CTTACAGTTT CAGAGTGCCT CGGGGACCTC CGTTGACTTC 
CTCCTCCACA CTGTTGGAGC AGAGGACTTT GGGAACTACA GCTGTGTCTA CTATGAGACA 
ACCATGTCCA ACAGGGGCTC ATATCTTAGT ACCCCCCTTA TGATCTGGGT GACTGACACA 
TTCCCCAGAC CATGGTTGTC TGCTGAGCCT AGTTCTGTGG TCACCATGGG GCAGAATGTT 
ACTCTATGGT GCCAAGGACC AGTCCATGGA GTTGGGTACA TTCTGCACAA AGAAGGAGAA 
GCCACTTCAA TGCAGCTTTG GGATTCCACT AGCAATGAAG GGGCATTCCC TATCATCAAT 
ATATCTGGTG CTAGCATAGG ACGTTATAGC TGCTGTTACC ACCCTGACTG GATGAGCCCT 
ATCAAGATAC AGCCTAGCAA CACTCTGGAA CTCATAGTCA CTGATAAGCC CCCTAAACCC 
TCTCTGTCAG CGTGGCCCAG CACTGTGTTC AAACTAGGAA AGGATATCAC CCTTCAGTGC 
CGAGGACCCC TGCCAGGAGT TGAATTCGTG CTAGAACACG ATGGAGAAGA GGCCCCCCAA 
CAGTTCTCAG AGGATGGAGA TTTTGTTATC GGCAACATGG AAGGGAAAGG CATTGGAAAC 
TACAGTTGCA GCTATCGGCT GCAGGCTTAC CCTGACATCT GGTCAGAGCC CAGCGACTCC 
CTGGAGCTGG TTGGAGCCGC AGGGCCTGTT GCTCAAGAGT GCACTGTGGG TAACATTGTC 
CGAAGTACCC TGATTGTGGT GGTTGTGGTA GCCTTGGGGA TAGTGCTAGC CATAGAGTGG 
AAGAAGTGGC CTCGACTTCG GACTAGGGGC TCTGAGACAG ATGGGAGAGA CCAGACCATA 
GTCCTTGAAG AGTGTAACCA AGATGGAGAA CCCGGCACTG CCACCAATTC TCCTTCATCT 
GCTTTGCAGG GTGTCTCAGT GGAACAAACA GTCCCAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The short mRNA isoform of the immunoglobulin superfamily, member 1 gene encodes an intracellular glycoprotein.
PloS one12(7)e0180731(2017)
Wang Y,Br?l? E,Silander T,Bak B,Joustra SD,Bernard DJ

Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat.
The Journal of endocrinology226(3)181-91(2015 Sep)
Joustra SD,Meijer OC,Heinen CA,Mol IM,Laghmani el H,Sengers RM,Carreno G,van Trotsenburg AS,Biermasz NR,Bernard DJ,Wit JM,Oostdijk W,van Pelt AM,Hamer G,Wagenaar GT

Modulation of activin signal transduction by inhibin B and inhibin-binding protein (INhBP).
Molecular endocrinology (Baltimore, Md.)15(4)668-79(2001 Apr)
Chapman SC,Woodruff TK

Inhibin binding protein in rats: alternative transcripts and regulation in the pituitary across the estrous cycle.
Molecular endocrinology (Baltimore, Md.)15(4)654-67(2001 Apr)
Bernard DJ,Woodruff TK

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Igsf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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