Ptk7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptk7
Entrez Gene ID	301242
Full Name	protein tyrosine kinase 7
General protein information	Preferred Names protein tyrosine kinase 7 Names inactive tyrosine-protein kinase 7 PTK7 protein tyrosine kinase 7 tyrosine-protein kinase-like 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596327.1	XP_017451816.1	inactive tyrosine-protein kinase 7 isoform X1
NM_001106889.1	NP_001100359.1	inactive tyrosine-protein kinase 7

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Protein tyrosine kinase 7 has a conserved role in Wnt/?-catenin canonical signalling.
Puppo F, Thom? V, Lhoumeau AC, Cibois M, Gangar A, Lembo F, Belotti E, Marchetto S, L?cine P, Pr?bet T, Sebbagh M, Shin WS, Lee ST, Kodjabachian L, Borg JP
EMBO reports12(1)43-9(2011 Jan)

The Wnt/planar cell polarity protein-tyrosine kinase-7 (PTK7) is a highly efficient proteolytic target of membrane type-1 matrix metalloproteinase: implications in cancer and embryogenesis.
Golubkov VS, Chekanov AV, Cieplak P, Aleshin AE, Chernov AV, Zhu W, Radichev IA, Zhang D, Dong PD, Strongin AY
The Journal of biological chemistry285(46)35740-9(2010 Nov)

The novel mouse mutant, chuzhoi, has disruption of Ptk7 protein and exhibits defects in neural tube, heart and lung development and abnormal planar cell polarity in the ear.
Paudyal A, Damrau C, Patterson VL, Ermakov A, Formstone C, Lalanne Z, Wells S, Lu X, Norris DP, Dean CH, Henderson DJ, Murdoch JN
BMC developmental biology1087(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ptk7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptk7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11070	NM_001106889.1 Latest version!	Rattus norvegicus protein tyrosine kinase 7 (Ptk7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$454.30 $649.00
ORa62683	XM_017596327.1 Latest version!	Rattus norvegicus protein tyrosine kinase 7 (Ptk7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptk7 ( NM_001106889.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100359.1 Rattus norvegicus protein tyrosine kinase 7 (Ptk7), mRNA.

Price & Availability↑

CloneID	ORa11070
Clone ID Related Accession (Same CDS sequence)	NM_001106889.1
Accession Version	NM_001106889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	inactive tyrosine-protein kinase 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473987.2. On or before Oct 4, 2007 this sequence version replaced XM_001066019.1, XM_217346.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGAGCTT TGCCAGGGTG CTGCTGGCAC CTCAGGACGT CGTTGTGGCA AGGAATGAGG 
AGGCCATGTT CCATTGCCAG TTTTCAGCTC AGCCGCCCCC GAGCCTGCAG TGGGTCTTTG 
AGGATGAGAC TCCCATCACG AACCGAAGCC GCCATGGTGT TTGCCAATGG CTCCCTGCTG 
CTGACCCAAG TCCGGCCACG GAACGCAGGA GTCTACCGTT GCATTGGTCA GGGACAGAGG 
GGTCCCCCCA TCGTCCTAGA AGCCACTCTT CACCTGGCAG AGATCGAAGA CATGCTGCCG 
TTGGAACCAC GGGTGTTCAT AGCTGGTGGT GAGGAACGTG TTGCCTGCCC AGCCCCCCAG 
GGTCTGCCTA CGCCCAGTGT CTGGTGGGAA CACGCTGGAG TCCGTCTTCC TGCTCATGGA 
AGGGTCCACC AGAAGGGCCT CGAGCTCGTG TTTGCCACTA TCGCTGAGAG TGACGCCGGT 
GTCTACACCT GCCACGCAGC CAACCTGGCT GGTCAGCGGC GACAGGATGT GAACATCACA 
GTGGCCACTG TTCCCACGTG GTTAAGGAAG CCCCAAGACA GCCAGCTGGA AGAGGGCAAA 
CCTGGCTACC TGCACTGTCT GACACAGGCC ACACCCAAGC CCACCGTCAT CTGGTACAGG 
AACCAGATGC TTATCTCGGA GGACTCACGC TTCGAAGTCT CAAAGAATGG GACCTTGCGC 
ATCAACAGTG TGGAGGTATA TGACGGGACA GTGTACCGCT GTGTGAGCAG CACCCCAGCT 
GGCAGCATCG AGGCCCAGGC CCGTGTCCAG GTGCTGGAAA AACTCAAGTT CACGCCACCG 
CCACAGCCAC AGCAATGCAT GGAGTTTGAT AAGGAGGCTA CGGTGCCCTG CTCAGCCACT 
GGCCGAGAGA AACCCACGGT TAAGTGGGTA CGGGCAGATG GGAGCAGCCT CCCCGAATGG 
GTGACAGACA ATGCGGGGAC CCTGCACTTT GCCCGAGTGA CCCGAGATGA CGCTGGCAAC 
TACACCTGCA TTGCGTCCAA TGAGCCACAG GGCCAGATCC GTGCCCATGT CCAGCTGACG 
GTGGCAGTTT TCATCACGTT CAAGGTGGAA CCGGAGCGTA CAACGGTGTA CCAGGGCCAC 
ACGGCGCTGC TGCGGTGTGA GGCGCAGGGG GATCCCAAGC CTCTTATTCA GTGGAAGGGC 
AAAGACCGAA TCCTGGACCC CACCAAGCTG GGACCCAGGA TGCACATCTT CCAGAACGGC 
TCCCTGGTGA TCCATGATGT GGCCCCTGAG GACTCAGGCT CCTACACCTG CATCGCTGGC 
AACAGCTGTA ACATCAGGCA CACAGAGGCC CCGCTCTTAG TCGTGGGTGG GCCTTTGCAG 
AATGGACAGC CGTCAGCAGA GATCCAGGAA GAAGTGGCGT TGACCAGCTT GGGCTCTGGC 
CCTACAGCCA CCAACAAGCG CCACAGCACT GGCGATAAGA TGCATTTCCC TCGGGCCAGC 
CTGCAGCCCA TCACCACTCT GGGGAAAAGC GAGTTCGGGG AGGTGTTCCT CGCAAAGGCT 
CAGGGCTTAG ACGAAGGCGC GACAGAGACC CTGGTGCTTG TGAAAAGCCT GCAGAACAAG 
GACGAGCAGC AGCAGCTGGA CTTCCGGAGG GAGTTTGAGA TGTTTGGGAA ACTGAACCAT 
GCCAACGTGG TGCGGCTCCT GGGGCTGTGC CGGGAGGCTG AGCCCCATTA CATGGTGCTG 
GAGTATGTGG ACCTGGGAGA TCTCAAACAG TTCCTGAGGA TTTCCAAGAA CAAGGATGAA 
AAGTTGAAGT CACAGCCCCT CAGCACCAAA CAGAAGGTGG CCCTGTGTTC CCAGGTGGCC 
CTGGGCATGG AACACTTGTC CAACAACCGC TTTGTGCACA AGGACTTAGC TGCTCGCAAC 
TGCCTGGTCA GCGCCCAGAG GCAGGTGAAG GTGTCCGCGC TGGGCCTCAG CAAGGATGTA 
TACAACAGTG AATACTACCA CTTCCGCCAG GCCTGGGTAC CTTTGCGTTG GATGTCCCCA 
GAGGCTGTCC TGGAGGGTGA CTTCTCCACC AAGTCTGACG TCTGGGCCTT TGGCGTGCTC 
ATGTGGGAAG TGTTCACTCA TGGAGAGATG CCCCATGGTG GACAGGGAGA TGATGAGGTG 
CTGGCGGACT TGCAGGCTGG GAAGGCTCGG CTCCCGCAGC CCGAAGGCTG CCCGTCTAAG 
CTCTACCGGC TGATGCAACG CTGCTGGGCC CTGAACCCCA AGGACCGGCC CTCCTTCAGC 
GAGATCGCTA GCACCCTGGG AGATGGCCCT GCAGACAGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100359.1
CDS	754..3081
Translation	MRALPGCCWHLRTSLWQGMRRPCSIASFQLSRPRACSGSLRMRLPSRTEAAMVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHGRVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVGGPLQNGQPSAEIQEEVALTSLGSGPTATNKRHSTGDKMHFPRASLQPITTLGKSEFGEVFLAKAQGLDEGATETLVLVKSLQNKDEQQQLDFRREFEMFGKLNHANVVRLLGLCREAEPHYMVLEYVDLGDLKQFLRISKNKDEKLKSQPLSTKQKVALCSQVALGMEHLSNNRFVHKDLAARNCLVSAQRQVKVSALGLSKDVYNSEYYHFRQAWVPLRWMSPEAVLEGDFSTKSDVWAFGVLMWEVFTHGEMPHGGQGDDEVLADLQAGKARLPQPEGCPSKLYRLMQRCWALNPKDRPSFSEIASTLGDGPADSKQ

Target ORF information:

RefSeq Version	NM_001106889.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 7 (Ptk7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106889.1

ORF Insert Sequence:

ATGAGAGCTT TGCCAGGGTG CTGCTGGCAC CTCAGGACGT CGTTGTGGCA AGGAATGAGG 
AGGCCATGTT CCATTGCCAG TTTTCAGCTC AGCCGCCCCC GAGCCTGCAG TGGGTCTTTG 
AGGATGAGAC TCCCATCACG AACCGAAGCC GCCATGGTGT TTGCCAATGG CTCCCTGCTG 
CTGACCCAAG TCCGGCCACG GAACGCAGGA GTCTACCGTT GCATTGGTCA GGGACAGAGG 
GGTCCCCCCA TCGTCCTAGA AGCCACTCTT CACCTGGCAG AGATCGAAGA CATGCTGCCG 
TTGGAACCAC GGGTGTTCAT AGCTGGTGGT GAGGAACGTG TTGCCTGCCC AGCCCCCCAG 
GGTCTGCCTA CGCCCAGTGT CTGGTGGGAA CACGCTGGAG TCCGTCTTCC TGCTCATGGA 
AGGGTCCACC AGAAGGGCCT CGAGCTCGTG TTTGCCACTA TCGCTGAGAG TGACGCCGGT 
GTCTACACCT GCCACGCAGC CAACCTGGCT GGTCAGCGGC GACAGGATGT GAACATCACA 
GTGGCCACTG TTCCCACGTG GTTAAGGAAG CCCCAAGACA GCCAGCTGGA AGAGGGCAAA 
CCTGGCTACC TGCACTGTCT GACACAGGCC ACACCCAAGC CCACCGTCAT CTGGTACAGG 
AACCAGATGC TTATCTCGGA GGACTCACGC TTCGAAGTCT CAAAGAATGG GACCTTGCGC 
ATCAACAGTG TGGAGGTATA TGACGGGACA GTGTACCGCT GTGTGAGCAG CACCCCAGCT 
GGCAGCATCG AGGCCCAGGC CCGTGTCCAG GTGCTGGAAA AACTCAAGTT CACGCCACCG 
CCACAGCCAC AGCAATGCAT GGAGTTTGAT AAGGAGGCTA CGGTGCCCTG CTCAGCCACT 
GGCCGAGAGA AACCCACGGT TAAGTGGGTA CGGGCAGATG GGAGCAGCCT CCCCGAATGG 
GTGACAGACA ATGCGGGGAC CCTGCACTTT GCCCGAGTGA CCCGAGATGA CGCTGGCAAC 
TACACCTGCA TTGCGTCCAA TGAGCCACAG GGCCAGATCC GTGCCCATGT CCAGCTGACG 
GTGGCAGTTT TCATCACGTT CAAGGTGGAA CCGGAGCGTA CAACGGTGTA CCAGGGCCAC 
ACGGCGCTGC TGCGGTGTGA GGCGCAGGGG GATCCCAAGC CTCTTATTCA GTGGAAGGGC 
AAAGACCGAA TCCTGGACCC CACCAAGCTG GGACCCAGGA TGCACATCTT CCAGAACGGC 
TCCCTGGTGA TCCATGATGT GGCCCCTGAG GACTCAGGCT CCTACACCTG CATCGCTGGC 
AACAGCTGTA ACATCAGGCA CACAGAGGCC CCGCTCTTAG TCGTGGGTGG GCCTTTGCAG 
AATGGACAGC CGTCAGCAGA GATCCAGGAA GAAGTGGCGT TGACCAGCTT GGGCTCTGGC 
CCTACAGCCA CCAACAAGCG CCACAGCACT GGCGATAAGA TGCATTTCCC TCGGGCCAGC 
CTGCAGCCCA TCACCACTCT GGGGAAAAGC GAGTTCGGGG AGGTGTTCCT CGCAAAGGCT 
CAGGGCTTAG ACGAAGGCGC GACAGAGACC CTGGTGCTTG TGAAAAGCCT GCAGAACAAG 
GACGAGCAGC AGCAGCTGGA CTTCCGGAGG GAGTTTGAGA TGTTTGGGAA ACTGAACCAT 
GCCAACGTGG TGCGGCTCCT GGGGCTGTGC CGGGAGGCTG AGCCCCATTA CATGGTGCTG 
GAGTATGTGG ACCTGGGAGA TCTCAAACAG TTCCTGAGGA TTTCCAAGAA CAAGGATGAA 
AAGTTGAAGT CACAGCCCCT CAGCACCAAA CAGAAGGTGG CCCTGTGTTC CCAGGTGGCC 
CTGGGCATGG AACACTTGTC CAACAACCGC TTTGTGCACA AGGACTTAGC TGCTCGCAAC 
TGCCTGGTCA GCGCCCAGAG GCAGGTGAAG GTGTCCGCGC TGGGCCTCAG CAAGGATGTA 
TACAACAGTG AATACTACCA CTTCCGCCAG GCCTGGGTAC CTTTGCGTTG GATGTCCCCA 
GAGGCTGTCC TGGAGGGTGA CTTCTCCACC AAGTCTGACG TCTGGGCCTT TGGCGTGCTC 
ATGTGGGAAG TGTTCACTCA TGGAGAGATG CCCCATGGTG GACAGGGAGA TGATGAGGTG 
CTGGCGGACT TGCAGGCTGG GAAGGCTCGG CTCCCGCAGC CCGAAGGCTG CCCGTCTAAG 
CTCTACCGGC TGATGCAACG CTGCTGGGCC CTGAACCCCA AGGACCGGCC CTCCTTCAGC 
GAGATCGCTA GCACCCTGGG AGATGGCCCT GCAGACAGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk7 ( XM_017596327.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451816.1 Rattus norvegicus protein tyrosine kinase 7 (Ptk7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62683
Clone ID Related Accession (Same CDS sequence)	XM_017596327.1
Accession Version	XM_017596327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inactive tyrosine-protein kinase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGGAGCCC GCCAGCTGCC TCTGCTCAGA GCCCTGCTTC TGCCGTTGCT GGCCGGTGCC 
CATTCTGCCA TCGTCTTTAT CAAGGAGCCA TCCTCCCAGG ATGCACTTCA AGGGCGCCGG 
GCGCTGCTCC GCTGTGAGGT CGAGGCTCCG GACCCCGTGC ACGTGTACTG GTTGCTCAAT 
GGCGTGCCTG TCCAGGACAC CGAGCGACGC TTCGCCCAAG GTAGCAGCCT GAGCTTTGCG 
GCTGTGGACC GGCTTCAAGA TTCTGGTGCC TTCCAGTGTG TGGCTCGGGA CAATATCACT 
GGAGAGGAAG CCCGCAGTGC CAATGCCTCC TTCAACATCA AATGGATCGA GGCGGGTCCT 
GTGGTTCTGA AGCATCCAGC CTCAGCAGCT GAGATCCAGC CGCAGACCCA GGTCACACTC 
CGATGTCACA TTGATGGGCA CCCTCGGCCT ACCTACCAGT GGTTCCGAGA CGGGACCCCC 
CTCTCTGATG ATCAGAGCAC CCACACAGTC AGCAGCAAGG AGAGGAACCT GACCCTACGA 
CCTGCCAGTC CCGAACATAG CGGCCTCTAC TCCTGCTGTG CCCACAATGC CTTTGGCCAG 
GCCTGCAGCA GCCAGAACTT CACCTTGAGT ATTGCAGATG AGAGCTTTGC CAGGGTGCTG 
CTGGCACCTC AGGACGTCGT TGTGGCAAGG AATGAGGAGG CCATGTTCCA TTGCCAGTTT 
TCAGCTCAGC CGCCCCCGAG CCTGCAGTGG GTCTTTGAGG ATGAGACTCC CATCACGAAC 
CGAAGCCGTC CCCCTCACCT CCGCAAAGCC ATGGTGTTTG CCAATGGCTC CCTGCTGCTG 
ACCCAAGTCC GGCCACGGAA CGCAGGAGTC TACCGTTGCA TTGGTCAGGG ACAGAGGGGT 
CCCCCCATCG TCCTAGAAGC CACTCTTCAC CTGGCAGAGA TCGAAGACAT GCTGCCGTTG 
GAACCACGGG TGTTCATAGC TGGTGGTGAG GAACGTGTTG CCTGCCCAGC CCCCCAGGGT 
CTGCCTACGC CCAGTGTCTG GTGGGAACAC GCTGGAGTCC GTCTTCCTGC TCATGGAAGG 
GTCCACCAGA AGGGCCTCGA GCTCGTGTTT GCCACTATCG CTGAGAGTGA CGCCGGTGTC 
TACACCTGCC ACGCAGCCAA CCTGGCTGGT CAGCGGCGAC AGGATGTGAA CATCACAGTG 
GCCACTGTTC CCACGTGGTT AAGGAAGCCC CAAGACAGCC AGCTGGAAGA GGGCAAACCT 
GGCTACCTGC ACTGTCTGAC ACAGGCCACA CCCAAGCCCA CCGTCATCTG GTACAGGAAC 
CAGATGCTTA TCTCGGAGGA CTCACGCTTC GAAGTCTCAA AGAATGGGAC CTTGCGCATC 
AACAGTGTGG AGGTATATGA CGGGACAGTG TACCGCTGTG TGAGCAGCAC CCCAGCTGGC 
AGCATCGAGG CCCAGGCCCG TGTCCAGGTG CTGGAAAAAC TCAAGTTCAC GCCACCGCCA 
CAGCCACAGC AATGCATGGA GTTTGATAAG GAGGCTACGG TGCCCTGCTC AGCCACTGGC 
CGAGAGAAAC CCACGGTTAA GTGGGTACGG GCAGATGGGA GCAGCCTCCC CGAATGGGTG 
ACAGACAATG CGGGGACCCT GCACTTTGCC CGAGTGACCC GAGATGACGC TGGCAACTAC 
ACCTGCATTG CGTCCAATGA GCCACAGGGC CAGATCCGTG CCCATGTCCA GCTGACGGTG 
GCAGTTTTCA TCACGTTCAA GGTGGAACCG GAGCGTACAA CGGTGTACCA GGGCCACACG 
GCGCTGCTGC GGTGTGAGGC GCAGGGGGAT CCCAAGCCTC TTATTCAGTG GAAGGGCAAA 
GACCGAATCC TGGACCCCAC CAAGCTGGGA CCCAGGATGC ACATCTTCCA GAACGGCTCC 
CTGGTGATCC ATGATGTGGC CCCTGAGGAC TCAGGCTCCT ACACCTGCAT CGCTGGCAAC 
AGCTGTAACA TCAGGCACAC AGAGGCCCCG CTCTTAGTCG TGGACAAGCC GCTGATGGAG 
GACTCAGAGG GCCCAGGCAG CCCTCCCCCG TACAAGATGA TCCAGACCAT CGGGCTGTCA 
GTGGGTGCAG CTGTAGCCTA TATCATAGCT GTACTGGGCC TCATGTTCTA CTGCAAGAAG 
AGGTGTAAAG CCAAGAGACT GCAGAAACAG CCTGAAGGGG AGGAGCCTGA GATGGAGTGC 
CTCAATGGTG GGTTCCCCTG TAAGGGGAGG AGCCTGAGGT GGAGTGCCTC ACTGGTGGGT 
TCCCCTGTAA GGGGAGGAGC CCGAGGTGGA GTGCCTCACT GGTGGGTTCC CCTGTCCACC 
AACAGCAGCG CCAATGCACT GGCGATTAAG GTGCATTTTC CTCCGGGCCG CCTGCAGCCC 
ATCACGCAGA CTCTGAAAAA TGCTCCCCTG CCTGATGGCT TGGGTGCCTC AAAAGCTTGT 
GTCACAAGAA AGGCCGTGGC TGCGTCTCTC TCTCTCAGCG CCTTCCTCTG CCAACATGAA 
TCTCTACCCA CAGGGAAAAG CGAGTTCGGG GAGGTGTTCC TCGCAAAGGC TCAGGGCTTA 
GACGAAGGCG CGACAGAGAC CCTGGTGCTT GTGAAAAGCC TGCAGAACAA GGACGAGCAG 
CAGCAGCTGG ACTTCCGGAG GGAGTTTGAG ATGTTTGGGA AACTGAACCA TGCCAACGTG 
GTGCGGCTCC TGGGGCTGTG CCGGGAGGCT GAGCCCCATT ACATGGTGCT GGAGTATGTG 
GACCTGGTAT GTTGTGGAGT GTCAGTGGAG AGGATTCTGG GGCTAGGGCA CCACAGAGGT 
CTTGGCTTTT ATTACTCGGG AGGCTGCGGC AAAGAACGGC CTTGGGCCAC ATTCGGTCTA 
CAGGTGGTCC CCTTGATCCT TAGTCCTGGG CTGTGCTCAC AAGCTGTTCT CTCTCGCCAG 
GTGGCCCTGT GTTCCCAGGT GGCCCTGGGC ATGGAACACT TGTCCAACAA CCGCTTTGTG 
CACAAGGACT TAGCTGCTCG CAACTGCCTG GTCAGCGCCC AGAGGCAGGT GAAGGTGTCC 
GCGCTGGGCC TCAGCAAGGA TGTATACAAC AGTGAATACT ACCACTTCCG CCAGGCCTGG 
GTACCTTTGC GTTGGATGTC CCCAGAGGCT GTCCTGGAGG GTGACTTCTC CACCAAGTCT 
GACGTCTGGG CCTTTGGCGT GCTCATGTGG GAAGTGTTCA CTCATGGAGA GATGCCCCAT 
GGTGGACAGG GAGATGATGA GGTGCTGGCG GACTTGCAGG CTGGGAAGGC TCGGCTCCCG 
CAGCCCGAAG GCTGCCCGTC TAAGCTCTAC CGGCTGATGC AACGCTGCTG GGCCCTGAAC 
CCCAAGGACC GGCCCTCCTT CAGCGAGATC GCTAGCACCC TGGGAGATGG CCCTGCAGAC 
AGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451816.1
CDS	163..3594
Translation	MGARQLPLLRALLLPLLAGAHSAIVFIKEPSSQDALQGRRALLRCEVEAPDPVHVYWLLNGVPVQDTERRFAQGSSLSFAAVDRLQDSGAFQCVARDNITGEEARSANASFNIKWIEAGPVVLKHPASAAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDDQSTHTVSSKERNLTLRPASPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVLLAPQDVVVARNEEAMFHCQFSAQPPPSLQWVFEDETPITNRSRPPHLRKAMVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHGRVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVDKPLMEDSEGPGSPPPYKMIQTIGLSVGAAVAYIIAVLGLMFYCKKRCKAKRLQKQPEGEEPEMECLNGGFPCKGRSLRWSASLVGSPVRGGARGGVPHWWVPLSTNSSANALAIKVHFPPGRLQPITQTLKNAPLPDGLGASKACVTRKAVAASLSLSAFLCQHESLPTGKSEFGEVFLAKAQGLDEGATETLVLVKSLQNKDEQQQLDFRREFEMFGKLNHANVVRLLGLCREAEPHYMVLEYVDLVCCGVSVERILGLGHHRGLGFYYSGGCGKERPWATFGLQVVPLILSPGLCSQAVLSRQVALCSQVALGMEHLSNNRFVHKDLAARNCLVSAQRQVKVSALGLSKDVYNSEYYHFRQAWVPLRWMSPEAVLEGDFSTKSDVWAFGVLMWEVFTHGEMPHGGQGDDEVLADLQAGKARLPQPEGCPSKLYRLMQRCWALNPKDRPSFSEIASTLGDGPADSKQ

Target ORF information:

RefSeq Version	XM_017596327.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 7 (Ptk7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596327.1

ORF Insert Sequence:

ATGGGAGCCC GCCAGCTGCC TCTGCTCAGA GCCCTGCTTC TGCCGTTGCT GGCCGGTGCC 
CATTCTGCCA TCGTCTTTAT CAAGGAGCCA TCCTCCCAGG ATGCACTTCA AGGGCGCCGG 
GCGCTGCTCC GCTGTGAGGT CGAGGCTCCG GACCCCGTGC ACGTGTACTG GTTGCTCAAT 
GGCGTGCCTG TCCAGGACAC CGAGCGACGC TTCGCCCAAG GTAGCAGCCT GAGCTTTGCG 
GCTGTGGACC GGCTTCAAGA TTCTGGTGCC TTCCAGTGTG TGGCTCGGGA CAATATCACT 
GGAGAGGAAG CCCGCAGTGC CAATGCCTCC TTCAACATCA AATGGATCGA GGCGGGTCCT 
GTGGTTCTGA AGCATCCAGC CTCAGCAGCT GAGATCCAGC CGCAGACCCA GGTCACACTC 
CGATGTCACA TTGATGGGCA CCCTCGGCCT ACCTACCAGT GGTTCCGAGA CGGGACCCCC 
CTCTCTGATG ATCAGAGCAC CCACACAGTC AGCAGCAAGG AGAGGAACCT GACCCTACGA 
CCTGCCAGTC CCGAACATAG CGGCCTCTAC TCCTGCTGTG CCCACAATGC CTTTGGCCAG 
GCCTGCAGCA GCCAGAACTT CACCTTGAGT ATTGCAGATG AGAGCTTTGC CAGGGTGCTG 
CTGGCACCTC AGGACGTCGT TGTGGCAAGG AATGAGGAGG CCATGTTCCA TTGCCAGTTT 
TCAGCTCAGC CGCCCCCGAG CCTGCAGTGG GTCTTTGAGG ATGAGACTCC CATCACGAAC 
CGAAGCCGTC CCCCTCACCT CCGCAAAGCC ATGGTGTTTG CCAATGGCTC CCTGCTGCTG 
ACCCAAGTCC GGCCACGGAA CGCAGGAGTC TACCGTTGCA TTGGTCAGGG ACAGAGGGGT 
CCCCCCATCG TCCTAGAAGC CACTCTTCAC CTGGCAGAGA TCGAAGACAT GCTGCCGTTG 
GAACCACGGG TGTTCATAGC TGGTGGTGAG GAACGTGTTG CCTGCCCAGC CCCCCAGGGT 
CTGCCTACGC CCAGTGTCTG GTGGGAACAC GCTGGAGTCC GTCTTCCTGC TCATGGAAGG 
GTCCACCAGA AGGGCCTCGA GCTCGTGTTT GCCACTATCG CTGAGAGTGA CGCCGGTGTC 
TACACCTGCC ACGCAGCCAA CCTGGCTGGT CAGCGGCGAC AGGATGTGAA CATCACAGTG 
GCCACTGTTC CCACGTGGTT AAGGAAGCCC CAAGACAGCC AGCTGGAAGA GGGCAAACCT 
GGCTACCTGC ACTGTCTGAC ACAGGCCACA CCCAAGCCCA CCGTCATCTG GTACAGGAAC 
CAGATGCTTA TCTCGGAGGA CTCACGCTTC GAAGTCTCAA AGAATGGGAC CTTGCGCATC 
AACAGTGTGG AGGTATATGA CGGGACAGTG TACCGCTGTG TGAGCAGCAC CCCAGCTGGC 
AGCATCGAGG CCCAGGCCCG TGTCCAGGTG CTGGAAAAAC TCAAGTTCAC GCCACCGCCA 
CAGCCACAGC AATGCATGGA GTTTGATAAG GAGGCTACGG TGCCCTGCTC AGCCACTGGC 
CGAGAGAAAC CCACGGTTAA GTGGGTACGG GCAGATGGGA GCAGCCTCCC CGAATGGGTG 
ACAGACAATG CGGGGACCCT GCACTTTGCC CGAGTGACCC GAGATGACGC TGGCAACTAC 
ACCTGCATTG CGTCCAATGA GCCACAGGGC CAGATCCGTG CCCATGTCCA GCTGACGGTG 
GCAGTTTTCA TCACGTTCAA GGTGGAACCG GAGCGTACAA CGGTGTACCA GGGCCACACG 
GCGCTGCTGC GGTGTGAGGC GCAGGGGGAT CCCAAGCCTC TTATTCAGTG GAAGGGCAAA 
GACCGAATCC TGGACCCCAC CAAGCTGGGA CCCAGGATGC ACATCTTCCA GAACGGCTCC 
CTGGTGATCC ATGATGTGGC CCCTGAGGAC TCAGGCTCCT ACACCTGCAT CGCTGGCAAC 
AGCTGTAACA TCAGGCACAC AGAGGCCCCG CTCTTAGTCG TGGACAAGCC GCTGATGGAG 
GACTCAGAGG GCCCAGGCAG CCCTCCCCCG TACAAGATGA TCCAGACCAT CGGGCTGTCA 
GTGGGTGCAG CTGTAGCCTA TATCATAGCT GTACTGGGCC TCATGTTCTA CTGCAAGAAG 
AGGTGTAAAG CCAAGAGACT GCAGAAACAG CCTGAAGGGG AGGAGCCTGA GATGGAGTGC 
CTCAATGGTG GGTTCCCCTG TAAGGGGAGG AGCCTGAGGT GGAGTGCCTC ACTGGTGGGT 
TCCCCTGTAA GGGGAGGAGC CCGAGGTGGA GTGCCTCACT GGTGGGTTCC CCTGTCCACC 
AACAGCAGCG CCAATGCACT GGCGATTAAG GTGCATTTTC CTCCGGGCCG CCTGCAGCCC 
ATCACGCAGA CTCTGAAAAA TGCTCCCCTG CCTGATGGCT TGGGTGCCTC AAAAGCTTGT 
GTCACAAGAA AGGCCGTGGC TGCGTCTCTC TCTCTCAGCG CCTTCCTCTG CCAACATGAA 
TCTCTACCCA CAGGGAAAAG CGAGTTCGGG GAGGTGTTCC TCGCAAAGGC TCAGGGCTTA 
GACGAAGGCG CGACAGAGAC CCTGGTGCTT GTGAAAAGCC TGCAGAACAA GGACGAGCAG 
CAGCAGCTGG ACTTCCGGAG GGAGTTTGAG ATGTTTGGGA AACTGAACCA TGCCAACGTG 
GTGCGGCTCC TGGGGCTGTG CCGGGAGGCT GAGCCCCATT ACATGGTGCT GGAGTATGTG 
GACCTGGTAT GTTGTGGAGT GTCAGTGGAG AGGATTCTGG GGCTAGGGCA CCACAGAGGT 
CTTGGCTTTT ATTACTCGGG AGGCTGCGGC AAAGAACGGC CTTGGGCCAC ATTCGGTCTA 
CAGGTGGTCC CCTTGATCCT TAGTCCTGGG CTGTGCTCAC AAGCTGTTCT CTCTCGCCAG 
GTGGCCCTGT GTTCCCAGGT GGCCCTGGGC ATGGAACACT TGTCCAACAA CCGCTTTGTG 
CACAAGGACT TAGCTGCTCG CAACTGCCTG GTCAGCGCCC AGAGGCAGGT GAAGGTGTCC 
GCGCTGGGCC TCAGCAAGGA TGTATACAAC AGTGAATACT ACCACTTCCG CCAGGCCTGG 
GTACCTTTGC GTTGGATGTC CCCAGAGGCT GTCCTGGAGG GTGACTTCTC CACCAAGTCT 
GACGTCTGGG CCTTTGGCGT GCTCATGTGG GAAGTGTTCA CTCATGGAGA GATGCCCCAT 
GGTGGACAGG GAGATGATGA GGTGCTGGCG GACTTGCAGG CTGGGAAGGC TCGGCTCCCG 
CAGCCCGAAG GCTGCCCGTC TAAGCTCTAC CGGCTGATGC AACGCTGCTG GGCCCTGAAC 
CCCAAGGACC GGCCCTCCTT CAGCGAGATC GCTAGCACCC TGGGAGATGG CCCTGCAGAC 
AGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Protein tyrosine kinase 7 has a conserved role in Wnt/?-catenin canonical signalling.
EMBO reports12(1)43-9(2011 Jan)
Puppo F,Thom? V,Lhoumeau AC,Cibois M,Gangar A,Lembo F,Belotti E,Marchetto S,L?cine P,Pr?bet T,Sebbagh M,Shin WS,Lee ST,Kodjabachian L,Borg JP

The Wnt/planar cell polarity protein-tyrosine kinase-7 (PTK7) is a highly efficient proteolytic target of membrane type-1 matrix metalloproteinase: implications in cancer and embryogenesis.
The Journal of biological chemistry285(46)35740-9(2010 Nov)
Golubkov VS,Chekanov AV,Cieplak P,Aleshin AE,Chernov AV,Zhu W,Radichev IA,Zhang D,Dong PD,Strongin AY

The novel mouse mutant, chuzhoi, has disruption of Ptk7 protein and exhibits defects in neural tube, heart and lung development and abnormal planar cell polarity in the ear.
BMC developmental biology1087(2010 Aug)
Paudyal A,Damrau C,Patterson VL,Ermakov A,Formstone C,Lalanne Z,Wells S,Lu X,Norris DP,Dean CH,Henderson DJ,Murdoch JN

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Ptk7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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