Rfx2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rfx2
Entrez Gene ID	301121
Full Name	regulatory factor X2
General protein information	Preferred Names regulatory factor X2 Names DNA-binding protein RFX2 regulatory factor X 2 regulatory factor X, 2 (influences HLA class II expression)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106877.1	NP_001100347.1	DNA-binding protein RFX2
XM_006244313.3	XP_006244375.1	DNA-binding protein RFX2 isoform X1

Pathways from BioSystems

Homologies

	RFX2	XP_001086864.2
Bos taurus (cattle)	RFX2	NP_001095642.1
Danio rerio (zebrafish)	rfx2	NP_001013296.1
Pan troglodytes (chimpanzee)	RFX2	XP_512310.3
Canis lupus familiaris (dog)	RFX2	XP_003432842.1
Mus musculus (house mouse)	Rfx2	NP_082063.1
Homo sapiens (human)	RFX2	NP_000626.2
Rattus norvegicus (Norway rat)	Rfx2	NP_001100347.1
Xenopus tropicalis (tropical clawed frog)	rfx2	NP_001116955.1
Gallus gallus (chicken)	RFX2	XP_004948801.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.
Wu Y, Hu X, Li Z, Wang M, Li S, Wang X, Lin X, Liao S, Zhang Z, Feng X, Wang S, Cui X, Wang Y, Gao F, Hess RA, Han C
Scientific reports620435(2016 Feb)

Rfx2 is required for spermatogenesis in the mouse.
Shawlot W, Vazquez-Chantada M, Wallingford JB, Finnell RH
Genesis (New York, N.Y. : 2000)(2015 Aug)

RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
Kistler WS, Baas D, Lemeille S, Paschaki M, Seguin-Estevez Q, Barras E, Ma W, Duteyrat JL, Morl? L, Durand B, Reith W
PLoS genetics11(7)e1005368(2015 Jul)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.
VanWert JM, Wolfe SA, Grimes SR
Journal of cellular biochemistry104(3)1087-101(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RFX2 and NF-Y binding to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes
Title: Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.

These data support the hypothesis that RFX2 expression is upregulated in spermatocytes where it participates in activating transcription of the H1t gene and other testis genes.
Title: Transcription factor RFX2 is abundant in rat testis and enriched in nuclei of primary spermatocytes where it appears to be required for transcription of the testis-specific histone H1t gene.

The following Rfx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rfx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02414	NM_001106877.1 Latest version!	Rattus norvegicus regulatory factor X2 (Rfx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa31889	XM_006244313.3 Latest version!	Rattus norvegicus regulatory factor X2 (Rfx2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rfx2 ( NM_001106877.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100347.1 Rattus norvegicus regulatory factor X2 (Rfx2), mRNA.

Price & Availability↑

CloneID	ORa02414
Clone ID Related Accession (Same CDS sequence)	NM_001106877.1
Accession Version	NM_001106877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	DNA-binding protein RFX2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474092.1. On or before Oct 4, 2007 this sequence version replaced XM_236777.4, XM_001055912.1, XM_001055859.1, XM_001055742.1, XM_001055800.1. ##Evidence-Data-START## Transcript exon combination :: BC166527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAACT CCGAGGGAGG AGCAGACTCG CCTGCCACAG TGGCTCTGCG TCCTGCAGCC 
CAGCCTGTAC CAGCCTCCCC ACAGAGGGTG CTGGTACAAG CCGCAGGCTC CACTCCCAAG 
GGTACCCCGA TGCAGACACT CACCCTCCCC AGAGTCCAGC CAGTGCCACC ACAGGTGCAG 
CACGTGTACC CCGCACAGGT GCAGTACGTG GAGGGTGGGG ATGCTGTCTA CGCCAACGGA 
GCCATCCGAG CCGCCTATAC CTACAACCCA GACCCTCAGC TCTACGCCCC CAGCAGCGCA 
GCTTCTTACT TTGAGACTCC AGGTGGCGCT CAGGTGACGG TGGCAGCCTC GTCCCCACCA 
GCGGTTCCCT CCCACGGCAT GGTGGGCATC ACCATGGATG TCTCAGGGAC CCCCATTGTG 
TCTGGCGCAG GAACCTACCT CATCCACGGG GGCATGGACA GCACAAGACA CTCCCTGGCC 
CACACTGCCC GCTCGTCACC TGCCACGCTC CAGTGGCTTC TGGACAATTA CGAGACAGCA 
GAGGGTGTGA GCCTGCCCCG CAGCTCCCTG TACAACCACT ACCTGCGCCA CTGCCAGGAG 
CACAAGCTGG AGCCAGTCAA TGCTGCATCC TTCGGCAAGC TCATCCGCTC TGTATTCATG 
GGCCTGCGCA CACGACGCTT GGGGACCAGG GGCAACTCAA AGTACCATTA CTATGGGATC 
CGCCTGAAGC CAGACTCACC ACTGAATCGA CTGCAGGAAG ACACACAGTA CATGGCCATG 
AGGCAGCAGC CCACGCACCA GAAGCCTAGG TATCGGCCAG CCCAGAAGTC AGACAGCCTC 
GGGGATGGCA GTGCTCACAG CAACATGCAC AGCACACCCG AGCAAGCCAT GGCCGCCCAG 
GGCCAGCATC ACCAGCAGTA CATAGATGTG TCCCACGTCT TCCCAGAGTT CCCAGCACCT 
GACCTAGGCA GCACGTTACT GCAGGAGAGT GTCACCCTTC ATGATGTGAA GGCCCTGCAG 
CTGGTGTATC GCAGGCACTG TGAGGCCACC TTAGATGTCG TCATGAACCT CCAGTTTCAG 
TACATTGAGA AGCTGTGGTT GTCCTTCTGG AACTGCAAGG CCACCTCCAG TGATGGCCGT 
GCCTCGCTGC CTGCCAGCGA CGAGGAGCCT GAAGTCACCC TCCTCCCCAA GGACAAGCTC 
ATCTCTCTGT GTAAGTGTGA GCCCATCCTG CAGTGGATGC GCAGCTGCGA TCACATCCTG 
TACCAGGCGC TGGTGGAGAC CCTCATCCCA GACGTGCTAC GGCCCGTGCC CAGTTCCCTC 
ACACAGGCCA TCAGGAACTT CGCCAAGAGC CTGGAGGGCT GGCTCATCAA TGCCATGAGT 
GGCTTCCCTC AGCAGGTCAT CCAGACCAAG GTGGGTGTTG TAAGTGCCTT TGCACAGACA 
CTGCGGCGCT ACACGTCCCT CAACCACCTG GCACAGGCAG CACGTGCCGT GCTACAGAAT 
ACGTCACAGA TTAACCAGAT GCTGAGTGAC CTCAACCGCG TGGACTTTGC CAATGTGCAG 
GAGCAGGCCT CATGGGTGTG CCAGTGTGAG GAGAGCCTGG TGCAGCGTTT GGAGCATGAC 
TTCAAGGTCA CCCTGCAGCA GCAGAGCTCG CTGGACCAAT GGGCCAGCTG GCTGGACAAT 
GTGGTCACCC AGGTACTGAA GCAGCATGCA GGCAGCCCCA GCTTCCCCAA GGCTGCACGG 
CAGTTCCTGC TCAAGTGGTC CTTCTACAGC TCCATGGTAA TCCGGGACCT GACCCTGCGC 
AGCGCTGCCA GCTTTGGCTC CTTCCACCTC ATCCGCCTGT TGTATGACGA GTACATGTTC 
TACCTGGTGG AGCACCGTGT GGCCCAGGCC ACCGGGGAGA CACCCATTGC TGTCATGGGC 
GAGTTCAATG ACCTGGCGTC CCTGTCGCTG ACCCTGCTGG ACAAAGAGGA CATTGGGGAC 
GGACACAGCA GTGAGGCAGA TGTGGATGGC CGCAGCCTGG GCGAGCCCCT GGTGAAGCGG 
GAACGGAGTG ACCCCAGCCA CCCACTGCAG GGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100347.1
CDS	147..2225
Translation	MQNSEGGADSPATVALRPAAQPVPASPQRVLVQAAGSTPKGTPMQTLTLPRVQPVPPQVQHVYPAQVQYVEGGDAVYANGAIRAAYTYNPDPQLYAPSSAASYFETPGGAQVTVAASSPPAVPSHGMVGITMDVSGTPIVSGAGTYLIHGGMDSTRHSLAHTARSSPATLQWLLDNYETAEGVSLPRSSLYNHYLRHCQEHKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGIRLKPDSPLNRLQEDTQYMAMRQQPTHQKPRYRPAQKSDSLGDGSAHSNMHSTPEQAMAAQGQHHQQYIDVSHVFPEFPAPDLGSTLLQESVTLHDVKALQLVYRRHCEATLDVVMNLQFQYIEKLWLSFWNCKATSSDGRASLPASDEEPEVTLLPKDKLISLCKCEPILQWMRSCDHILYQALVETLIPDVLRPVPSSLTQAIRNFAKSLEGWLINAMSGFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESLVQRLEHDFKVTLQQQSSLDQWASWLDNVVTQVLKQHAGSPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFNDLASLSLTLLDKEDIGDGHSSEADVDGRSLGEPLVKRERSDPSHPLQGI

Target ORF information:

RefSeq Version	NM_001106877.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulatory factor X2 (Rfx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106877.1

ORF Insert Sequence:

ATGCAGAACT CCGAGGGAGG AGCAGACTCG CCTGCCACAG TGGCTCTGCG TCCTGCAGCC 
CAGCCTGTAC CAGCCTCCCC ACAGAGGGTG CTGGTACAAG CCGCAGGCTC CACTCCCAAG 
GGTACCCCGA TGCAGACACT CACCCTCCCC AGAGTCCAGC CAGTGCCACC ACAGGTGCAG 
CACGTGTACC CCGCACAGGT GCAGTACGTG GAGGGTGGGG ATGCTGTCTA CGCCAACGGA 
GCCATCCGAG CCGCCTATAC CTACAACCCA GACCCTCAGC TCTACGCCCC CAGCAGCGCA 
GCTTCTTACT TTGAGACTCC AGGTGGCGCT CAGGTGACGG TGGCAGCCTC GTCCCCACCA 
GCGGTTCCCT CCCACGGCAT GGTGGGCATC ACCATGGATG TCTCAGGGAC CCCCATTGTG 
TCTGGCGCAG GAACCTACCT CATCCACGGG GGCATGGACA GCACAAGACA CTCCCTGGCC 
CACACTGCCC GCTCGTCACC TGCCACGCTC CAGTGGCTTC TGGACAATTA CGAGACAGCA 
GAGGGTGTGA GCCTGCCCCG CAGCTCCCTG TACAACCACT ACCTGCGCCA CTGCCAGGAG 
CACAAGCTGG AGCCAGTCAA TGCTGCATCC TTCGGCAAGC TCATCCGCTC TGTATTCATG 
GGCCTGCGCA CACGACGCTT GGGGACCAGG GGCAACTCAA AGTACCATTA CTATGGGATC 
CGCCTGAAGC CAGACTCACC ACTGAATCGA CTGCAGGAAG ACACACAGTA CATGGCCATG 
AGGCAGCAGC CCACGCACCA GAAGCCTAGG TATCGGCCAG CCCAGAAGTC AGACAGCCTC 
GGGGATGGCA GTGCTCACAG CAACATGCAC AGCACACCCG AGCAAGCCAT GGCCGCCCAG 
GGCCAGCATC ACCAGCAGTA CATAGATGTG TCCCACGTCT TCCCAGAGTT CCCAGCACCT 
GACCTAGGCA GCACGTTACT GCAGGAGAGT GTCACCCTTC ATGATGTGAA GGCCCTGCAG 
CTGGTGTATC GCAGGCACTG TGAGGCCACC TTAGATGTCG TCATGAACCT CCAGTTTCAG 
TACATTGAGA AGCTGTGGTT GTCCTTCTGG AACTGCAAGG CCACCTCCAG TGATGGCCGT 
GCCTCGCTGC CTGCCAGCGA CGAGGAGCCT GAAGTCACCC TCCTCCCCAA GGACAAGCTC 
ATCTCTCTGT GTAAGTGTGA GCCCATCCTG CAGTGGATGC GCAGCTGCGA TCACATCCTG 
TACCAGGCGC TGGTGGAGAC CCTCATCCCA GACGTGCTAC GGCCCGTGCC CAGTTCCCTC 
ACACAGGCCA TCAGGAACTT CGCCAAGAGC CTGGAGGGCT GGCTCATCAA TGCCATGAGT 
GGCTTCCCTC AGCAGGTCAT CCAGACCAAG GTGGGTGTTG TAAGTGCCTT TGCACAGACA 
CTGCGGCGCT ACACGTCCCT CAACCACCTG GCACAGGCAG CACGTGCCGT GCTACAGAAT 
ACGTCACAGA TTAACCAGAT GCTGAGTGAC CTCAACCGCG TGGACTTTGC CAATGTGCAG 
GAGCAGGCCT CATGGGTGTG CCAGTGTGAG GAGAGCCTGG TGCAGCGTTT GGAGCATGAC 
TTCAAGGTCA CCCTGCAGCA GCAGAGCTCG CTGGACCAAT GGGCCAGCTG GCTGGACAAT 
GTGGTCACCC AGGTACTGAA GCAGCATGCA GGCAGCCCCA GCTTCCCCAA GGCTGCACGG 
CAGTTCCTGC TCAAGTGGTC CTTCTACAGC TCCATGGTAA TCCGGGACCT GACCCTGCGC 
AGCGCTGCCA GCTTTGGCTC CTTCCACCTC ATCCGCCTGT TGTATGACGA GTACATGTTC 
TACCTGGTGG AGCACCGTGT GGCCCAGGCC ACCGGGGAGA CACCCATTGC TGTCATGGGC 
GAGTTCAATG ACCTGGCGTC CCTGTCGCTG ACCCTGCTGG ACAAAGAGGA CATTGGGGAC 
GGACACAGCA GTGAGGCAGA TGTGGATGGC CGCAGCCTGG GCGAGCCCCT GGTGAAGCGG 
GAACGGAGTG ACCCCAGCCA CCCACTGCAG GGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfx2 ( XM_006244313.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244375.1 Rattus norvegicus regulatory factor X2 (Rfx2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31889
Clone ID Related Accession (Same CDS sequence)	XM_006244313.3
Accession Version	XM_006244313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA-binding protein RFX2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAACT CCGAGGGAGG AGCAGACTCG CCTGCCACAG TGGCTCTGCG TCCTGCAGCC 
CAGCCTGTAC CAGCCTCCCC ACAGAGGGTG CTGGTACAAG CCGCAGGCTC CACTCCCAAG 
GGTACCCCGA TGCAGACACT CACCCTCCCC AGAGTCCAGC CAGTGCCACC ACAGGTGCAG 
CACGTGTACC CCGCACAGGT GCAGTACGTG GAGGGTGGGG ATGCTGTCTA CGCCAACGGA 
GCCATCCGAG CCGCCTATAC CTACAACCCA GACCCTCAGC TCTACGCCCC CAGCAGCGCA 
GCTTCTTACT TTGAGACTCC AGGTGGCGCT CAGGTGACGG TGGCAGCCTC GTCCCCACCA 
GCGGTTCCCT CCCACGGCAT GGTGGGCATC ACCATGGATG TCTCAGGGAC CCCCATTGTG 
TCTGGCGCAG GAACCTACCT CATCCACGGG GGCATGGACA GCACAAGACA CTCCCTGGCC 
CACACTGCCC GCTCGTCACC TGCCACGCTT GAAATGGCGA TTGAGACTCT CCAAAAAAGT 
GAAGGCCTCG CCCCCCACAA AGGCGGCCTG CTCAACAGCC ATCTCCAGTG GCTTCTGGAC 
AATTACGAGA CAGCAGAGGG TGTGAGCCTG CCCCGCAGCT CCCTGTACAA CCACTACCTG 
CGCCACTGCC AGGAGCACAA GCTGGAGCCA GTCAATGCTG CATCCTTCGG CAAGCTCATC 
CGCTCTGTAT TCATGGGCCT GCGCACACGA CGCTTGGGGA CCAGGGGCAA CTCAAAGTAC 
CATTACTATG GGATCCGCCT GAAGCCAGAC TCACCACTGA ATCGACTGCA GGAAGACACA 
CAGTACATGG CCATGAGGCA GCAGCCCACG CACCAGAAGC CTAGGTATCG GCCAGCCCAG 
AAGTCAGACA GCCTCGGGGA TGGCAGTGCT CACAGCAACA TGCACAGCAC ACCCGAGCAA 
GCCATGGCCG CCCAGGGCCA GCATCACCAG CAGTACATAG ATGTGTCCCA CGTCTTCCCA 
GAGTTCCCAG CACCTGACCT AGGCAGCACG TTACTGCAGG AGAGTGTCAC CCTTCATGAT 
GTGAAGGCCC TGCAGCTGGT GTATCGCAGG CACTGTGAGG CCACCTTAGA TGTCGTCATG 
AACCTCCAGT TTCAGTACAT TGAGAAGCTG TGGTTGTCCT TCTGGAACTG CAAGGCCACC 
TCCAGTGATG GCCGTGCCTC GCTGCCTGCC AGCGACGAGG AGCCTGAAGT CACCCTCCTC 
CCCAAGGACA AGCTCATCTC TCTGTGTAAG TGTGAGCCCA TCCTGCAGTG GATGCGCAGC 
TGCGATCACA TCCTGTACCA GGCGCTGGTG GAGACCCTCA TCCCAGACGT GCTACGGCCC 
GTGCCCAGTT CCCTCACACA GGCCATCAGG AACTTCGCCA AGAGCCTGGA GGGCTGGCTC 
ATCAATGCCA TGAGTGGCTT CCCTCAGCAG GTCATCCAGA CCAAGGTGGG TGTTGTAAGT 
GCCTTTGCAC AGACACTGCG GCGCTACACG TCCCTCAACC ACCTGGCACA GGCAGCACGT 
GCCGTGCTAC AGAATACGTC ACAGATTAAC CAGATGCTGA GTGACCTCAA CCGCGTGGAC 
TTTGCCAATG TGCAGGAGCA GGCCTCATGG GTGTGCCAGT GTGAGGAGAG CCTGGTGCAG 
CGTTTGGAGC ATGACTTCAA GGTCACCCTG CAGCAGCAGA GCTCGCTGGA CCAATGGGCC 
AGCTGGCTGG ACAATGTGGT CACCCAGGTA CTGAAGCAGC ATGCAGGCAG CCCCAGCTTC 
CCCAAGGCTG CACGGCAGTT CCTGCTCAAG TGGTCCTTCT ACAGCTCCAT GGTAATCCGG 
GACCTGACCC TGCGCAGCGC TGCCAGCTTT GGCTCCTTCC ACCTCATCCG CCTGTTGTAT 
GACGAGTACA TGTTCTACCT GGTGGAGCAC CGTGTGGCCC AGGCCACCGG GGAGACACCC 
ATTGCTGTCA TGGGCGAGTT CAATGACCTG GCGTCCCTGT CGCTGACCCT GCTGGACAAA 
GAGGACATTG GGGACGGACA CAGCAGTGAG GCAGATGTGG ATGGCCGCAG CCTGGGCGAG 
CCCCTGGTGA AGCGGGAACG GAGTGACCCC AGCCACCCAC TGCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244375.1
CDS	163..2316
Translation	MQNSEGGADSPATVALRPAAQPVPASPQRVLVQAAGSTPKGTPMQTLTLPRVQPVPPQVQHVYPAQVQYVEGGDAVYANGAIRAAYTYNPDPQLYAPSSAASYFETPGGAQVTVAASSPPAVPSHGMVGITMDVSGTPIVSGAGTYLIHGGMDSTRHSLAHTARSSPATLEMAIETLQKSEGLAPHKGGLLNSHLQWLLDNYETAEGVSLPRSSLYNHYLRHCQEHKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGIRLKPDSPLNRLQEDTQYMAMRQQPTHQKPRYRPAQKSDSLGDGSAHSNMHSTPEQAMAAQGQHHQQYIDVSHVFPEFPAPDLGSTLLQESVTLHDVKALQLVYRRHCEATLDVVMNLQFQYIEKLWLSFWNCKATSSDGRASLPASDEEPEVTLLPKDKLISLCKCEPILQWMRSCDHILYQALVETLIPDVLRPVPSSLTQAIRNFAKSLEGWLINAMSGFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESLVQRLEHDFKVTLQQQSSLDQWASWLDNVVTQVLKQHAGSPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFNDLASLSLTLLDKEDIGDGHSSEADVDGRSLGEPLVKRERSDPSHPLQGI

Target ORF information:

RefSeq Version	XM_006244313.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulatory factor X2 (Rfx2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244313.3

ORF Insert Sequence:

ATGCAGAACT CCGAGGGAGG AGCAGACTCG CCTGCCACAG TGGCTCTGCG TCCTGCAGCC 
CAGCCTGTAC CAGCCTCCCC ACAGAGGGTG CTGGTACAAG CCGCAGGCTC CACTCCCAAG 
GGTACCCCGA TGCAGACACT CACCCTCCCC AGAGTCCAGC CAGTGCCACC ACAGGTGCAG 
CACGTGTACC CCGCACAGGT GCAGTACGTG GAGGGTGGGG ATGCTGTCTA CGCCAACGGA 
GCCATCCGAG CCGCCTATAC CTACAACCCA GACCCTCAGC TCTACGCCCC CAGCAGCGCA 
GCTTCTTACT TTGAGACTCC AGGTGGCGCT CAGGTGACGG TGGCAGCCTC GTCCCCACCA 
GCGGTTCCCT CCCACGGCAT GGTGGGCATC ACCATGGATG TCTCAGGGAC CCCCATTGTG 
TCTGGCGCAG GAACCTACCT CATCCACGGG GGCATGGACA GCACAAGACA CTCCCTGGCC 
CACACTGCCC GCTCGTCACC TGCCACGCTT GAAATGGCGA TTGAGACTCT CCAAAAAAGT 
GAAGGCCTCG CCCCCCACAA AGGCGGCCTG CTCAACAGCC ATCTCCAGTG GCTTCTGGAC 
AATTACGAGA CAGCAGAGGG TGTGAGCCTG CCCCGCAGCT CCCTGTACAA CCACTACCTG 
CGCCACTGCC AGGAGCACAA GCTGGAGCCA GTCAATGCTG CATCCTTCGG CAAGCTCATC 
CGCTCTGTAT TCATGGGCCT GCGCACACGA CGCTTGGGGA CCAGGGGCAA CTCAAAGTAC 
CATTACTATG GGATCCGCCT GAAGCCAGAC TCACCACTGA ATCGACTGCA GGAAGACACA 
CAGTACATGG CCATGAGGCA GCAGCCCACG CACCAGAAGC CTAGGTATCG GCCAGCCCAG 
AAGTCAGACA GCCTCGGGGA TGGCAGTGCT CACAGCAACA TGCACAGCAC ACCCGAGCAA 
GCCATGGCCG CCCAGGGCCA GCATCACCAG CAGTACATAG ATGTGTCCCA CGTCTTCCCA 
GAGTTCCCAG CACCTGACCT AGGCAGCACG TTACTGCAGG AGAGTGTCAC CCTTCATGAT 
GTGAAGGCCC TGCAGCTGGT GTATCGCAGG CACTGTGAGG CCACCTTAGA TGTCGTCATG 
AACCTCCAGT TTCAGTACAT TGAGAAGCTG TGGTTGTCCT TCTGGAACTG CAAGGCCACC 
TCCAGTGATG GCCGTGCCTC GCTGCCTGCC AGCGACGAGG AGCCTGAAGT CACCCTCCTC 
CCCAAGGACA AGCTCATCTC TCTGTGTAAG TGTGAGCCCA TCCTGCAGTG GATGCGCAGC 
TGCGATCACA TCCTGTACCA GGCGCTGGTG GAGACCCTCA TCCCAGACGT GCTACGGCCC 
GTGCCCAGTT CCCTCACACA GGCCATCAGG AACTTCGCCA AGAGCCTGGA GGGCTGGCTC 
ATCAATGCCA TGAGTGGCTT CCCTCAGCAG GTCATCCAGA CCAAGGTGGG TGTTGTAAGT 
GCCTTTGCAC AGACACTGCG GCGCTACACG TCCCTCAACC ACCTGGCACA GGCAGCACGT 
GCCGTGCTAC AGAATACGTC ACAGATTAAC CAGATGCTGA GTGACCTCAA CCGCGTGGAC 
TTTGCCAATG TGCAGGAGCA GGCCTCATGG GTGTGCCAGT GTGAGGAGAG CCTGGTGCAG 
CGTTTGGAGC ATGACTTCAA GGTCACCCTG CAGCAGCAGA GCTCGCTGGA CCAATGGGCC 
AGCTGGCTGG ACAATGTGGT CACCCAGGTA CTGAAGCAGC ATGCAGGCAG CCCCAGCTTC 
CCCAAGGCTG CACGGCAGTT CCTGCTCAAG TGGTCCTTCT ACAGCTCCAT GGTAATCCGG 
GACCTGACCC TGCGCAGCGC TGCCAGCTTT GGCTCCTTCC ACCTCATCCG CCTGTTGTAT 
GACGAGTACA TGTTCTACCT GGTGGAGCAC CGTGTGGCCC AGGCCACCGG GGAGACACCC 
ATTGCTGTCA TGGGCGAGTT CAATGACCTG GCGTCCCTGT CGCTGACCCT GCTGGACAAA 
GAGGACATTG GGGACGGACA CAGCAGTGAG GCAGATGTGG ATGGCCGCAG CCTGGGCGAG 
CCCCTGGTGA AGCGGGAACG GAGTGACCCC AGCCACCCAC TGCAGGGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.
Scientific reports620435(2016 Feb)
Wu Y,Hu X,Li Z,Wang M,Li S,Wang X,Lin X,Liao S,Zhang Z,Feng X,Wang S,Cui X,Wang Y,Gao F,Hess RA,Han C

Rfx2 is required for spermatogenesis in the mouse.
Genesis (New York, N.Y. : 2000)(2015 Aug)
Shawlot W,Vazquez-Chantada M,Wallingford JB,Finnell RH

RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
PLoS genetics11(7)e1005368(2015 Jul)
Kistler WS,Baas D,Lemeille S,Paschaki M,Seguin-Estevez Q,Barras E,Ma W,Duteyrat JL,Morl? L,Durand B,Reith W

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.
Journal of cellular biochemistry104(3)1087-101(2008 Jun)
VanWert JM,Wolfe SA,Grimes SR

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Rfx2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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