Nckipsd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nckipsd
Entrez Gene ID	301009
Full Name	NCK interacting protein with SH3 domain
General protein information	Preferred Names NCK interacting protein with SH3 domain Names NCK-interacting protein with SH3 domain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766509.2	XP_008764731.1	NCK-interacting protein with SH3 domain isoform X2
NM_001106857.1	NP_001100327.1	NCK-interacting protein with SH3 domain
XM_006243754.3	XP_006243816.1	NCK-interacting protein with SH3 domain isoform X2
XM_006243753.3	XP_006243815.1	NCK-interacting protein with SH3 domain isoform X1

Pathways from BioSystems

Homologies

	NCKIPSD	XP_001111973.1
Danio rerio (zebrafish)	nckipsd	XP_002666143.3
Xenopus tropicalis (tropical clawed frog)	nckipsd	NP_001123414.1
Homo sapiens (human)	NCKIPSD	NP_057537.1
Rattus norvegicus (Norway rat)	Nckipsd	NP_001100327.1
Gallus gallus (chicken)	NCKIPSD	XP_001233774.3
Pan troglodytes (chimpanzee)	NCKIPSD	XP_516443.3
Bos taurus (cattle)	NCKIPSD	NP_001178167.1
Mus musculus (house mouse)	Nckipsd	NP_109654.2

Gene Ontology
Bibliography

Related articles in PubMed

SPIN90 dephosphorylation is required for cofilin-mediated actin depolymerization in NMDA-stimulated hippocampal neurons.
Cho IH, Lee MJ, Kim DH, Kim B, Bae J, Choi KY, Kim SM, Huh YH, Lee KH, Kim CH, Song WK
Cellular and molecular life sciences : CMLS70(22)4369-83(2013 Nov)

Control of growth cone motility and neurite outgrowth by SPIN90.
Kim SM, Bae J, Cho IH, Choi KY, Park YJ, Ryu JH, Chun JS, Song WK
Experimental cell research317(16)2276-87(2011 Oct)

Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry.
Fang L, Wang X, Yamoah K, Chen PL, Pan ZQ, Huang L
Journal of proteome research7(11)4914-25(2008 Nov)

Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts.
Kim SH, Choi HJ, Lee KW, Hong NH, Sung BH, Choi KY, Kim SM, Chang S, Eom SH, Song WK
Genes to cells : devoted to molecular & cellular mechanisms11(10)1197-211(2006 Oct)

SPIN90/WISH interacts with PSD-95 and regulates dendritic spinogenesis via an N-WASP-independent mechanism.
Lee S, Lee K, Hwang S, Kim SH, Song WK, Park ZY, Chang S
The EMBO journal25(20)4983-95(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPIN90 plays critical roles in controlling growth cone dynamics and neurite outgrowth during neuronal development.
Title: Control of growth cone motility and neurite outgrowth by SPIN90.

SPIN90-PRD, a syndapin-binding domain, significantly inhibited endocytosis, and the inhibition was reversed by co-expression of syndapin.
Title: Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts.

SPIN90/WISH is a downstream effector of PSD-95-dependent synaptic remodeling.
Title: SPIN90/WISH interacts with PSD-95 and regulates dendritic spinogenesis via an N-WASP-independent mechanism.

90 kDa SH3 protein interacting with Nck (SPIN90) can modulate the interactions of dynamin I with other endocytic proteins that cooperate in the coated vesicle formation, thus regulating synaptic vesicle endocytosis.
Title: Interaction of SPIN90 with dynamin I and its participation in synaptic vesicle endocytosis.

The following Nckipsd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nckipsd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05023	NM_001106857.1 Latest version!	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa31563	XM_008766509.2 Latest version!	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa31563	XM_006243754.3 Latest version!	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa31562	XM_006243753.3 Latest version!	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nckipsd ( NM_001106857.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100327.1 Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), mRNA.

Price & Availability↑

CloneID	ORa05023
Clone ID Related Accession (Same CDS sequence)	NM_001106857.1
Accession Version	NM_001106857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	NCK-interacting protein with SH3 domain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 4, 2007 this sequence version replaced XM_001075337.1, XM_238555.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTACCGCG CGCTGTATGC GTTCCGCTCC GCGGAGCCCA ACGCCATGGC GTTCGCTGCA 
GGCGAAACCT TCCTGGTGCT GGAGCGCAGC AGCACGCACT GGTGGTTAGC GGCACGGGCG 
CGCAGTGGCG AGACCGGGTA CGTGCCGCCT GCCTACCTGC ATCGCCTGCA GGGCATGGAG 
CAAGATGTCC TCCAAGCTAT TGACCGGGCC ATTGAGGCTG TGCACAACAC AGCCATGCGA 
GATGGTGGCA AGTACAGCCT GGAACAGCGT GGAGTCCTTC AGAAGCTTAT TCATCATCGG 
AAAGAGACCT TGTCTCGAAG GGGCACCTCA GCTGCCAGTG CTTCGGTTAT GACCCCATCC 
ACCAGTGACC ACCATTTGGA CACTGCTGTA TCTAGGCAGC CCAATGGGGT GTGTCGAACT 
GGGTTTGAAC GGCAGCATAG CCTACCCAGT TCTGAGCATC TCGGGACAGA TGGAGCCCTC 
TACCAGGTCC CACCACAGCC TCGGCGAGCA GCACCTGCCA CCCCACCCCC ACCAGTGAAG 
CGCCGAGACC GTGAGGCCCT GGTGATCTCA GGGAGTGGTG GCCGCACAGC AGTACCCTCT 
GGAGGTAGTT CTGTGTCTAG TGGCTCTTCA GTCAGCAGCA CCTCCATGGA CACACTCTAC 
ACTGGCTCTA GCCCCTCTGA GCTGGGTCCC AGCTGCTCAC CCACACCTCC GCCTGTACCT 
CGCCGAAGTG CCCACACTAC AGTGTCCCAG GCCCAGCCCT CTCCCTCCAA GGCACCATCC 
CCGGAGCCCC CTGCTGAGGA AGTGGCAGCT GAAACAAACT CAGCCCCTGA TGACCTCGAA 
GCTCTGGATG CCCTGAGCCC AGAGACCACA GAGGAGAAGC CAGCTACTGA GACAGTTGTG 
CCAAGGACCA TTGGGGCAGA GCTGATGGAA CTTGTTCGGA GAAACACTGG TCTGAGCCAC 
GAATTATGTC GTGTGGCCAT TGGTGTCGTG GTGGGTCACA TCCAGGCCTC CGTACCAGCC 
AGCTCGCCTG TCATGGAGCA GGTCCTCCTC TCGCTGGTAG AGGGCAAGGA CCTGAGCACA 
GCCCTACCCT CGGGGCAGGT CTGCCATGAC CAGCAGAGAC TGGAGGTGAT CTTTGCAGAC 
CTGGCTAGAA GGAAGGATGA CGCCCAGCAG CGCAGCTGGG CTCTTTACGA GGATGAGGAT 
GTCATCCGCT GCTACCTTGA GGAACTGTTG CACATCCTGA CGGATGCAGA TCCTGAAGTT 
TGCAAGAAAA TGTGCAAAAG GAGTGACTTC GAATCTGTCC TAGCCTTGGT GGCCTATTAC 
CAAATGGAAC ACCGAGCGTC ACTTCGGCTG CTGCTCCTCA AGTGCTTCGG CGCCATGTGC 
AGCCTGGACG CAGCAATCAT CTCTACTCTG GTCTCTTCTG TGCTACCTGT GGAATTGGCA 
CGGGACATGC AGACCGACAC ACAGGAAGCT CTGTTATTCC GCCCTTGTCC TGGCCATGGT 
CTTCTCCATG GGAGAGGCGG TGCCCTACGC ACACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100327.1
CDS	96..1634
Translation	MYRALYAFRSAEPNAMAFAAGETFLVLERSSTHWWLAARARSGETGYVPPAYLHRLQGMEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSAASASVMTPSTSDHHLDTAVSRQPNGVCRTGFERQHSLPSSEHLGTDGALYQVPPQPRRAAPATPPPPVKRRDREALVISGSGGRTAVPSGGSSVSSGSSVSSTSMDTLYTGSSPSELGPSCSPTPPPVPRRSAHTTVSQAQPSPSKAPSPEPPAEEVAAETNSAPDDLEALDALSPETTEEKPATETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQASVPASSPVMEQVLLSLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEVCKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQEALLFRPCPGHGLLHGRGGALRTL

Target ORF information:

RefSeq Version	NM_001106857.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106857.1

ORF Insert Sequence:

ATGTACCGCG CGCTGTATGC GTTCCGCTCC GCGGAGCCCA ACGCCATGGC GTTCGCTGCA 
GGCGAAACCT TCCTGGTGCT GGAGCGCAGC AGCACGCACT GGTGGTTAGC GGCACGGGCG 
CGCAGTGGCG AGACCGGGTA CGTGCCGCCT GCCTACCTGC ATCGCCTGCA GGGCATGGAG 
CAAGATGTCC TCCAAGCTAT TGACCGGGCC ATTGAGGCTG TGCACAACAC AGCCATGCGA 
GATGGTGGCA AGTACAGCCT GGAACAGCGT GGAGTCCTTC AGAAGCTTAT TCATCATCGG 
AAAGAGACCT TGTCTCGAAG GGGCACCTCA GCTGCCAGTG CTTCGGTTAT GACCCCATCC 
ACCAGTGACC ACCATTTGGA CACTGCTGTA TCTAGGCAGC CCAATGGGGT GTGTCGAACT 
GGGTTTGAAC GGCAGCATAG CCTACCCAGT TCTGAGCATC TCGGGACAGA TGGAGCCCTC 
TACCAGGTCC CACCACAGCC TCGGCGAGCA GCACCTGCCA CCCCACCCCC ACCAGTGAAG 
CGCCGAGACC GTGAGGCCCT GGTGATCTCA GGGAGTGGTG GCCGCACAGC AGTACCCTCT 
GGAGGTAGTT CTGTGTCTAG TGGCTCTTCA GTCAGCAGCA CCTCCATGGA CACACTCTAC 
ACTGGCTCTA GCCCCTCTGA GCTGGGTCCC AGCTGCTCAC CCACACCTCC GCCTGTACCT 
CGCCGAAGTG CCCACACTAC AGTGTCCCAG GCCCAGCCCT CTCCCTCCAA GGCACCATCC 
CCGGAGCCCC CTGCTGAGGA AGTGGCAGCT GAAACAAACT CAGCCCCTGA TGACCTCGAA 
GCTCTGGATG CCCTGAGCCC AGAGACCACA GAGGAGAAGC CAGCTACTGA GACAGTTGTG 
CCAAGGACCA TTGGGGCAGA GCTGATGGAA CTTGTTCGGA GAAACACTGG TCTGAGCCAC 
GAATTATGTC GTGTGGCCAT TGGTGTCGTG GTGGGTCACA TCCAGGCCTC CGTACCAGCC 
AGCTCGCCTG TCATGGAGCA GGTCCTCCTC TCGCTGGTAG AGGGCAAGGA CCTGAGCACA 
GCCCTACCCT CGGGGCAGGT CTGCCATGAC CAGCAGAGAC TGGAGGTGAT CTTTGCAGAC 
CTGGCTAGAA GGAAGGATGA CGCCCAGCAG CGCAGCTGGG CTCTTTACGA GGATGAGGAT 
GTCATCCGCT GCTACCTTGA GGAACTGTTG CACATCCTGA CGGATGCAGA TCCTGAAGTT 
TGCAAGAAAA TGTGCAAAAG GAGTGACTTC GAATCTGTCC TAGCCTTGGT GGCCTATTAC 
CAAATGGAAC ACCGAGCGTC ACTTCGGCTG CTGCTCCTCA AGTGCTTCGG CGCCATGTGC 
AGCCTGGACG CAGCAATCAT CTCTACTCTG GTCTCTTCTG TGCTACCTGT GGAATTGGCA 
CGGGACATGC AGACCGACAC ACAGGAAGCT CTGTTATTCC GCCCTTGTCC TGGCCATGGT 
CTTCTCCATG GGAGAGGCGG TGCCCTACGC ACACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckipsd ( XM_008766509.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764731.1 Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa31563
Clone ID Related Accession (Same CDS sequence)	XM_008766509.2 , XM_006243754.3
Accession Version	XM_008766509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NCK-interacting protein with SH3 domain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG ATGTCCTCCA AGCTATTGAC CGGGCCATTG AGGCTGTGCA CAACACAGCC 
ATGCGAGATG GTGGCAAGTA CAGCCTGGAA CAGCGTGGAG TCCTTCAGAA GCTTATTCAT 
CATCGGAAAG AGACCTTGTC TCGAAGGGGC ACCTCAGCTG CCAGTGCTTC GGTTATGACC 
CCATCCACCA GTGACCACCA TTTGGACACT GCTGTATCTA GGCAGCCCAA TGGGGTGTGT 
CGAACTGGGT TTGAACGGCA GCATAGCCTA CCCAGTTCTG AGCATCTCGG GACAGATGGA 
GCCCTCTACC AGGTCCCACC ACAGCCTCGG CGAGCAGCAC CTGCCACCCC ACCCCCACCA 
GTGAAGCGCC GAGACCGTGA GGCCCTGGTG ATCTCAGGGA GTGGTGGCCG CACAGCAGTA 
CCCTCTGGAG GTAGTTCTGT GTCTAGTGGC TCTTCAGTCA GCAGCACCTC CATGGACACA 
CTCTACACTG GCTCTAGCCC CTCTGAGCTG GGTCCCAGCT GCTCACCCAC ACCTCCGCCT 
GTACCTCGCC GAAGTGCCCA CACTACAGTG TCCCAGGCCC AGCCCTCTCC CTCCAAGGCA 
CCATCCCCGG AGCCCCCTGC TGAGGAAGTG GCAGCTGAAA CAAACTCAGC CCCTGATGAC 
CTCGAAGCTC TGGATGCCCT GAGCCCAGAG ACCACAGAGG AGAAGCCAGC TACTGAGACA 
GTTGTGCCAA GGACCATTGG GGCAGAGCTG ATGGAACTTG TTCGGAGAAA CACTGGTCTG 
AGCCACGAAT TATGTCGTGT GGCCATTGGT GTCGTGGTGG GTCACATCCA GGCCTCCGTA 
CCAGCCAGCT CGCCTGTCAT GGAGCAGGTC CTCCTCTCGC TGGTAGAGGG CAAGGACCTG 
AGCACAGCCC TACCCTCGGG GCAGGTCTGC CATGACCAGC AGAGACTGGA GGTGATCTTT 
GCAGACCTGG CTAGAAGGAA GGATGACGCC CAGCAGCGCA GCTGGGCTCT TTACGAGGAT 
GAGGATGTCA TCCGCTGCTA CCTTGAGGAA CTGTTGCACA TCCTGACGGA TGCAGATCCT 
GAAGTTTGCA AGAAAATGTG CAAAAGGAGT GACTTCGAAT CTGTCCTAGC CTTGGTGGCC 
TATTACCAAA TGGAACACCG AGCGTCACTT CGGCTGCTGC TCCTCAAGTG CTTCGGCGCC 
ATGTGCAGCC TGGACGCAGC AATCATCTCT ACTCTGGTCT CTTCTGTGCT ACCTGTGGAA 
TTGGCACGGG ACATGCAGAC CGACACACAG GACCACCAGA AGCTCTGTTA TTCCGCCCTT 
GTCCTGGCCA TGGTCTTCTC CATGGGAGAG GCGGTGCCCT ACGCACACTA TGAACACCTG 
GGTACACCCT TTGCCCAGTT CCTACTGAGC ATCGTTGAGG ATGGCCTGCC CCTGGACGCT 
ACAGAGCAGC TGCCAGACCT CTGCATGAAC CTGCTTCTGG CTCTCAACCT GCACCTGACA 
GCTCCTGACC AGAATGTCAT CATGGCTGCC TTAAGCAAGC ACACCAACGT GAAGATCTTC 
TCAGAGAAGC TGCTTTTGCT TCTGAACAGA GGGGACGATC CTGTGCGTAT CTTCAGACAT 
GAGCCCCAGC CGCCACACTC TGTCCTCAAG TTCCTGCAGG ACGTGTTCAG CAGCGCTGCC 
ACGGCCTCCA TCTTCTACCA CACAGACATG ATGGCGCTCA TTGACATCAC TGTGCGCCAG 
ATTGCCGACC TGTCACCTGG AGACAAGCTA CGCATGGAGT ACCTCTCCCT GATGCATGCC 
GTGGTCCGCT CCACACCCTA CCTCCAGCAC CGGCATCGGC TGTCTGACCT GCAGGCTACA 
CTGCGGCGCA TCCTGACTGA GGAAGAGACC TCACCCCAGT GCCAGATGGA CCGCATGATT 
GTCCAAGAGA TGTACAAGGA GTTTCCGGAC CTGGGAGAGG TCCCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764731.1
CDS	406..2376
Translation	MEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSAASASVMTPSTSDHHLDTAVSRQPNGVCRTGFERQHSLPSSEHLGTDGALYQVPPQPRRAAPATPPPPVKRRDREALVISGSGGRTAVPSGGSSVSSGSSVSSTSMDTLYTGSSPSELGPSCSPTPPPVPRRSAHTTVSQAQPSPSKAPSPEPPAEEVAAETNSAPDDLEALDALSPETTEEKPATETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQASVPASSPVMEQVLLSLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEVCKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPLDATEQLPDLCMNLLLALNLHLTAPDQNVIMAALSKHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSAATASIFYHTDMMALIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEETSPQCQMDRMIVQEMYKEFPDLGEVPS

Target ORF information:

RefSeq Version	XM_008766509.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766509.2

ORF Insert Sequence:

ATGGAGCAAG ATGTCCTCCA AGCTATTGAC CGGGCCATTG AGGCTGTGCA CAACACAGCC 
ATGCGAGATG GTGGCAAGTA CAGCCTGGAA CAGCGTGGAG TCCTTCAGAA GCTTATTCAT 
CATCGGAAAG AGACCTTGTC TCGAAGGGGC ACCTCAGCTG CCAGTGCTTC GGTTATGACC 
CCATCCACCA GTGACCACCA TTTGGACACT GCTGTATCTA GGCAGCCCAA TGGGGTGTGT 
CGAACTGGGT TTGAACGGCA GCATAGCCTA CCCAGTTCTG AGCATCTCGG GACAGATGGA 
GCCCTCTACC AGGTCCCACC ACAGCCTCGG CGAGCAGCAC CTGCCACCCC ACCCCCACCA 
GTGAAGCGCC GAGACCGTGA GGCCCTGGTG ATCTCAGGGA GTGGTGGCCG CACAGCAGTA 
CCCTCTGGAG GTAGTTCTGT GTCTAGTGGC TCTTCAGTCA GCAGCACCTC CATGGACACA 
CTCTACACTG GCTCTAGCCC CTCTGAGCTG GGTCCCAGCT GCTCACCCAC ACCTCCGCCT 
GTACCTCGCC GAAGTGCCCA CACTACAGTG TCCCAGGCCC AGCCCTCTCC CTCCAAGGCA 
CCATCCCCGG AGCCCCCTGC TGAGGAAGTG GCAGCTGAAA CAAACTCAGC CCCTGATGAC 
CTCGAAGCTC TGGATGCCCT GAGCCCAGAG ACCACAGAGG AGAAGCCAGC TACTGAGACA 
GTTGTGCCAA GGACCATTGG GGCAGAGCTG ATGGAACTTG TTCGGAGAAA CACTGGTCTG 
AGCCACGAAT TATGTCGTGT GGCCATTGGT GTCGTGGTGG GTCACATCCA GGCCTCCGTA 
CCAGCCAGCT CGCCTGTCAT GGAGCAGGTC CTCCTCTCGC TGGTAGAGGG CAAGGACCTG 
AGCACAGCCC TACCCTCGGG GCAGGTCTGC CATGACCAGC AGAGACTGGA GGTGATCTTT 
GCAGACCTGG CTAGAAGGAA GGATGACGCC CAGCAGCGCA GCTGGGCTCT TTACGAGGAT 
GAGGATGTCA TCCGCTGCTA CCTTGAGGAA CTGTTGCACA TCCTGACGGA TGCAGATCCT 
GAAGTTTGCA AGAAAATGTG CAAAAGGAGT GACTTCGAAT CTGTCCTAGC CTTGGTGGCC 
TATTACCAAA TGGAACACCG AGCGTCACTT CGGCTGCTGC TCCTCAAGTG CTTCGGCGCC 
ATGTGCAGCC TGGACGCAGC AATCATCTCT ACTCTGGTCT CTTCTGTGCT ACCTGTGGAA 
TTGGCACGGG ACATGCAGAC CGACACACAG GACCACCAGA AGCTCTGTTA TTCCGCCCTT 
GTCCTGGCCA TGGTCTTCTC CATGGGAGAG GCGGTGCCCT ACGCACACTA TGAACACCTG 
GGTACACCCT TTGCCCAGTT CCTACTGAGC ATCGTTGAGG ATGGCCTGCC CCTGGACGCT 
ACAGAGCAGC TGCCAGACCT CTGCATGAAC CTGCTTCTGG CTCTCAACCT GCACCTGACA 
GCTCCTGACC AGAATGTCAT CATGGCTGCC TTAAGCAAGC ACACCAACGT GAAGATCTTC 
TCAGAGAAGC TGCTTTTGCT TCTGAACAGA GGGGACGATC CTGTGCGTAT CTTCAGACAT 
GAGCCCCAGC CGCCACACTC TGTCCTCAAG TTCCTGCAGG ACGTGTTCAG CAGCGCTGCC 
ACGGCCTCCA TCTTCTACCA CACAGACATG ATGGCGCTCA TTGACATCAC TGTGCGCCAG 
ATTGCCGACC TGTCACCTGG AGACAAGCTA CGCATGGAGT ACCTCTCCCT GATGCATGCC 
GTGGTCCGCT CCACACCCTA CCTCCAGCAC CGGCATCGGC TGTCTGACCT GCAGGCTACA 
CTGCGGCGCA TCCTGACTGA GGAAGAGACC TCACCCCAGT GCCAGATGGA CCGCATGATT 
GTCCAAGAGA TGTACAAGGA GTTTCCGGAC CTGGGAGAGG TCCCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckipsd ( XM_006243754.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243816.1 Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31563
Clone ID Related Accession (Same CDS sequence)	XM_008766509.2 , XM_006243754.3
Accession Version	XM_006243754.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NCK-interacting protein with SH3 domain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG ATGTCCTCCA AGCTATTGAC CGGGCCATTG AGGCTGTGCA CAACACAGCC 
ATGCGAGATG GTGGCAAGTA CAGCCTGGAA CAGCGTGGAG TCCTTCAGAA GCTTATTCAT 
CATCGGAAAG AGACCTTGTC TCGAAGGGGC ACCTCAGCTG CCAGTGCTTC GGTTATGACC 
CCATCCACCA GTGACCACCA TTTGGACACT GCTGTATCTA GGCAGCCCAA TGGGGTGTGT 
CGAACTGGGT TTGAACGGCA GCATAGCCTA CCCAGTTCTG AGCATCTCGG GACAGATGGA 
GCCCTCTACC AGGTCCCACC ACAGCCTCGG CGAGCAGCAC CTGCCACCCC ACCCCCACCA 
GTGAAGCGCC GAGACCGTGA GGCCCTGGTG ATCTCAGGGA GTGGTGGCCG CACAGCAGTA 
CCCTCTGGAG GTAGTTCTGT GTCTAGTGGC TCTTCAGTCA GCAGCACCTC CATGGACACA 
CTCTACACTG GCTCTAGCCC CTCTGAGCTG GGTCCCAGCT GCTCACCCAC ACCTCCGCCT 
GTACCTCGCC GAAGTGCCCA CACTACAGTG TCCCAGGCCC AGCCCTCTCC CTCCAAGGCA 
CCATCCCCGG AGCCCCCTGC TGAGGAAGTG GCAGCTGAAA CAAACTCAGC CCCTGATGAC 
CTCGAAGCTC TGGATGCCCT GAGCCCAGAG ACCACAGAGG AGAAGCCAGC TACTGAGACA 
GTTGTGCCAA GGACCATTGG GGCAGAGCTG ATGGAACTTG TTCGGAGAAA CACTGGTCTG 
AGCCACGAAT TATGTCGTGT GGCCATTGGT GTCGTGGTGG GTCACATCCA GGCCTCCGTA 
CCAGCCAGCT CGCCTGTCAT GGAGCAGGTC CTCCTCTCGC TGGTAGAGGG CAAGGACCTG 
AGCACAGCCC TACCCTCGGG GCAGGTCTGC CATGACCAGC AGAGACTGGA GGTGATCTTT 
GCAGACCTGG CTAGAAGGAA GGATGACGCC CAGCAGCGCA GCTGGGCTCT TTACGAGGAT 
GAGGATGTCA TCCGCTGCTA CCTTGAGGAA CTGTTGCACA TCCTGACGGA TGCAGATCCT 
GAAGTTTGCA AGAAAATGTG CAAAAGGAGT GACTTCGAAT CTGTCCTAGC CTTGGTGGCC 
TATTACCAAA TGGAACACCG AGCGTCACTT CGGCTGCTGC TCCTCAAGTG CTTCGGCGCC 
ATGTGCAGCC TGGACGCAGC AATCATCTCT ACTCTGGTCT CTTCTGTGCT ACCTGTGGAA 
TTGGCACGGG ACATGCAGAC CGACACACAG GACCACCAGA AGCTCTGTTA TTCCGCCCTT 
GTCCTGGCCA TGGTCTTCTC CATGGGAGAG GCGGTGCCCT ACGCACACTA TGAACACCTG 
GGTACACCCT TTGCCCAGTT CCTACTGAGC ATCGTTGAGG ATGGCCTGCC CCTGGACGCT 
ACAGAGCAGC TGCCAGACCT CTGCATGAAC CTGCTTCTGG CTCTCAACCT GCACCTGACA 
GCTCCTGACC AGAATGTCAT CATGGCTGCC TTAAGCAAGC ACACCAACGT GAAGATCTTC 
TCAGAGAAGC TGCTTTTGCT TCTGAACAGA GGGGACGATC CTGTGCGTAT CTTCAGACAT 
GAGCCCCAGC CGCCACACTC TGTCCTCAAG TTCCTGCAGG ACGTGTTCAG CAGCGCTGCC 
ACGGCCTCCA TCTTCTACCA CACAGACATG ATGGCGCTCA TTGACATCAC TGTGCGCCAG 
ATTGCCGACC TGTCACCTGG AGACAAGCTA CGCATGGAGT ACCTCTCCCT GATGCATGCC 
GTGGTCCGCT CCACACCCTA CCTCCAGCAC CGGCATCGGC TGTCTGACCT GCAGGCTACA 
CTGCGGCGCA TCCTGACTGA GGAAGAGACC TCACCCCAGT GCCAGATGGA CCGCATGATT 
GTCCAAGAGA TGTACAAGGA GTTTCCGGAC CTGGGAGAGG TCCCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243816.1
CDS	612..2582
Translation	MEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSAASASVMTPSTSDHHLDTAVSRQPNGVCRTGFERQHSLPSSEHLGTDGALYQVPPQPRRAAPATPPPPVKRRDREALVISGSGGRTAVPSGGSSVSSGSSVSSTSMDTLYTGSSPSELGPSCSPTPPPVPRRSAHTTVSQAQPSPSKAPSPEPPAEEVAAETNSAPDDLEALDALSPETTEEKPATETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQASVPASSPVMEQVLLSLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEVCKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPLDATEQLPDLCMNLLLALNLHLTAPDQNVIMAALSKHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSAATASIFYHTDMMALIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEETSPQCQMDRMIVQEMYKEFPDLGEVPS

Target ORF information:

RefSeq Version	XM_006243754.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243754.3

ORF Insert Sequence:

ATGGAGCAAG ATGTCCTCCA AGCTATTGAC CGGGCCATTG AGGCTGTGCA CAACACAGCC 
ATGCGAGATG GTGGCAAGTA CAGCCTGGAA CAGCGTGGAG TCCTTCAGAA GCTTATTCAT 
CATCGGAAAG AGACCTTGTC TCGAAGGGGC ACCTCAGCTG CCAGTGCTTC GGTTATGACC 
CCATCCACCA GTGACCACCA TTTGGACACT GCTGTATCTA GGCAGCCCAA TGGGGTGTGT 
CGAACTGGGT TTGAACGGCA GCATAGCCTA CCCAGTTCTG AGCATCTCGG GACAGATGGA 
GCCCTCTACC AGGTCCCACC ACAGCCTCGG CGAGCAGCAC CTGCCACCCC ACCCCCACCA 
GTGAAGCGCC GAGACCGTGA GGCCCTGGTG ATCTCAGGGA GTGGTGGCCG CACAGCAGTA 
CCCTCTGGAG GTAGTTCTGT GTCTAGTGGC TCTTCAGTCA GCAGCACCTC CATGGACACA 
CTCTACACTG GCTCTAGCCC CTCTGAGCTG GGTCCCAGCT GCTCACCCAC ACCTCCGCCT 
GTACCTCGCC GAAGTGCCCA CACTACAGTG TCCCAGGCCC AGCCCTCTCC CTCCAAGGCA 
CCATCCCCGG AGCCCCCTGC TGAGGAAGTG GCAGCTGAAA CAAACTCAGC CCCTGATGAC 
CTCGAAGCTC TGGATGCCCT GAGCCCAGAG ACCACAGAGG AGAAGCCAGC TACTGAGACA 
GTTGTGCCAA GGACCATTGG GGCAGAGCTG ATGGAACTTG TTCGGAGAAA CACTGGTCTG 
AGCCACGAAT TATGTCGTGT GGCCATTGGT GTCGTGGTGG GTCACATCCA GGCCTCCGTA 
CCAGCCAGCT CGCCTGTCAT GGAGCAGGTC CTCCTCTCGC TGGTAGAGGG CAAGGACCTG 
AGCACAGCCC TACCCTCGGG GCAGGTCTGC CATGACCAGC AGAGACTGGA GGTGATCTTT 
GCAGACCTGG CTAGAAGGAA GGATGACGCC CAGCAGCGCA GCTGGGCTCT TTACGAGGAT 
GAGGATGTCA TCCGCTGCTA CCTTGAGGAA CTGTTGCACA TCCTGACGGA TGCAGATCCT 
GAAGTTTGCA AGAAAATGTG CAAAAGGAGT GACTTCGAAT CTGTCCTAGC CTTGGTGGCC 
TATTACCAAA TGGAACACCG AGCGTCACTT CGGCTGCTGC TCCTCAAGTG CTTCGGCGCC 
ATGTGCAGCC TGGACGCAGC AATCATCTCT ACTCTGGTCT CTTCTGTGCT ACCTGTGGAA 
TTGGCACGGG ACATGCAGAC CGACACACAG GACCACCAGA AGCTCTGTTA TTCCGCCCTT 
GTCCTGGCCA TGGTCTTCTC CATGGGAGAG GCGGTGCCCT ACGCACACTA TGAACACCTG 
GGTACACCCT TTGCCCAGTT CCTACTGAGC ATCGTTGAGG ATGGCCTGCC CCTGGACGCT 
ACAGAGCAGC TGCCAGACCT CTGCATGAAC CTGCTTCTGG CTCTCAACCT GCACCTGACA 
GCTCCTGACC AGAATGTCAT CATGGCTGCC TTAAGCAAGC ACACCAACGT GAAGATCTTC 
TCAGAGAAGC TGCTTTTGCT TCTGAACAGA GGGGACGATC CTGTGCGTAT CTTCAGACAT 
GAGCCCCAGC CGCCACACTC TGTCCTCAAG TTCCTGCAGG ACGTGTTCAG CAGCGCTGCC 
ACGGCCTCCA TCTTCTACCA CACAGACATG ATGGCGCTCA TTGACATCAC TGTGCGCCAG 
ATTGCCGACC TGTCACCTGG AGACAAGCTA CGCATGGAGT ACCTCTCCCT GATGCATGCC 
GTGGTCCGCT CCACACCCTA CCTCCAGCAC CGGCATCGGC TGTCTGACCT GCAGGCTACA 
CTGCGGCGCA TCCTGACTGA GGAAGAGACC TCACCCCAGT GCCAGATGGA CCGCATGATT 
GTCCAAGAGA TGTACAAGGA GTTTCCGGAC CTGGGAGAGG TCCCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckipsd ( XM_006243753.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243815.1 Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X1, mRNA.

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CloneID	ORa31562
Clone ID Related Accession (Same CDS sequence)	XM_006243753.3
Accession Version	XM_006243753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NCK-interacting protein with SH3 domain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGCG CGCTGTATGC GTTCCGCTCC GCGGAGCCCA ACGCCATGGC GTTCGCTGCA 
GGCGAAACCT TCCTGGTGCT GGAGCGCAGC AGCACGCACT GGTGGTTAGC GGCACGGGCG 
CGCAGTGGCG AGACCGGGTA CGTGCCGCCT GCCTACCTGC ATCGCCTGCA GGGCATGGAG 
CAAGATGTCC TCCAAGCTAT TGACCGGGCC ATTGAGGCTG TGCACAACAC AGCCATGCGA 
GATGGTGGCA AGTACAGCCT GGAACAGCGT GGAGTCCTTC AGAAGCTTAT TCATCATCGG 
AAAGAGACCT TGTCTCGAAG GGGCACCTCA GCTGCCAGTG CTTCGGTTAT GACCCCATCC 
ACCAGTGACC ACCATTTGGA CACTGCTGTA TCTAGGCAGC CCAATGGGGT GTGTCGAACT 
GGGTTTGAAC GGCAGCATAG CCTACCCAGT TCTGAGCATC TCGGGACAGA TGGAGCCCTC 
TACCAGGTCC CACCACAGCC TCGGCGAGCA GCACCTGCCA CCCCACCCCC ACCAGTGAAG 
CGCCGAGACC GTGAGGCCCT GGTGATCTCA GGGAGTGGTG GCCGCACAGC AGTACCCTCT 
GGAGGTAGTT CTGTGTCTAG TGGCTCTTCA GTCAGCAGCA CCTCCATGGA CACACTCTAC 
ACTGGCTCTA GCCCCTCTGA GCTGGGTCCC AGCTGCTCAC CCACACCTCC GCCTGTACCT 
CGCCGAAGTG CCCACACTAC AGTGTCCCAG GCCCAGCCCT CTCCCTCCAA GGCACCATCC 
CCGGAGCCCC CTGCTGAGGA AGTGGCAGCT GAAACAAACT CAGCCCCTGA TGACCTCGAA 
GCTCTGGATG CCCTGAGCCC AGAGACCACA GAGGAGAAGC CAGCTACTGA GACAGTTGTG 
CCAAGGACCA TTGGGGCAGA GCTGATGGAA CTTGTTCGGA GAAACACTGG TCTGAGCCAC 
GAATTATGTC GTGTGGCCAT TGGTGTCGTG GTGGGTCACA TCCAGGCCTC CGTACCAGCC 
AGCTCGCCTG TCATGGAGCA GGTCCTCCTC TCGCTGGTAG AGGGCAAGGA CCTGAGCACA 
GCCCTACCCT CGGGGCAGGT CTGCCATGAC CAGCAGAGAC TGGAGGTGAT CTTTGCAGAC 
CTGGCTAGAA GGAAGGATGA CGCCCAGCAG CGCAGCTGGG CTCTTTACGA GGATGAGGAT 
GTCATCCGCT GCTACCTTGA GGAACTGTTG CACATCCTGA CGGATGCAGA TCCTGAAGTT 
TGCAAGAAAA TGTGCAAAAG GAGTGACTTC GAATCTGTCC TAGCCTTGGT GGCCTATTAC 
CAAATGGAAC ACCGAGCGTC ACTTCGGCTG CTGCTCCTCA AGTGCTTCGG CGCCATGTGC 
AGCCTGGACG CAGCAATCAT CTCTACTCTG GTCTCTTCTG TGCTACCTGT GGAATTGGCA 
CGGGACATGC AGACCGACAC ACAGGACCAC CAGAAGCTCT GTTATTCCGC CCTTGTCCTG 
GCCATGGTCT TCTCCATGGG AGAGGCGGTG CCCTACGCAC ACTATGAACA CCTGGGTACA 
CCCTTTGCCC AGTTCCTACT GAGCATCGTT GAGGATGGCC TGCCCCTGGA CGCTACAGAG 
CAGCTGCCAG ACCTCTGCAT GAACCTGCTT CTGGCTCTCA ACCTGCACCT GACAGCTCCT 
GACCAGAATG TCATCATGGC TGCCTTAAGC AAGCACACCA ACGTGAAGAT CTTCTCAGAG 
AAGCTGCTTT TGCTTCTGAA CAGAGGGGAC GATCCTGTGC GTATCTTCAG ACATGAGCCC 
CAGCCGCCAC ACTCTGTCCT CAAGTTCCTG CAGGACGTGT TCAGCAGCGC TGCCACGGCC 
TCCATCTTCT ACCACACAGA CATGATGGCG CTCATTGACA TCACTGTGCG CCAGATTGCC 
GACCTGTCAC CTGGAGACAA GCTACGCATG GAGTACCTCT CCCTGATGCA TGCCGTGGTC 
CGCTCCACAC CCTACCTCCA GCACCGGCAT CGGCTGTCTG ACCTGCAGGC TACACTGCGG 
CGCATCCTGA CTGAGGAAGA GACCTCACCC CAGTGCCAGA TGGACCGCAT GATTGTCCAA 
GAGATGTACA AGGAGTTTCC GGACCTGGGA GAGGTCCCCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243815.1
CDS	111..2255
Translation	MYRALYAFRSAEPNAMAFAAGETFLVLERSSTHWWLAARARSGETGYVPPAYLHRLQGMEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSAASASVMTPSTSDHHLDTAVSRQPNGVCRTGFERQHSLPSSEHLGTDGALYQVPPQPRRAAPATPPPPVKRRDREALVISGSGGRTAVPSGGSSVSSGSSVSSTSMDTLYTGSSPSELGPSCSPTPPPVPRRSAHTTVSQAQPSPSKAPSPEPPAEEVAAETNSAPDDLEALDALSPETTEEKPATETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQASVPASSPVMEQVLLSLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEVCKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPLDATEQLPDLCMNLLLALNLHLTAPDQNVIMAALSKHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSAATASIFYHTDMMALIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEETSPQCQMDRMIVQEMYKEFPDLGEVPS

Target ORF information:

RefSeq Version	XM_006243753.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NCK interacting protein with SH3 domain (Nckipsd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243753.3

ORF Insert Sequence:

ATGTACCGCG CGCTGTATGC GTTCCGCTCC GCGGAGCCCA ACGCCATGGC GTTCGCTGCA 
GGCGAAACCT TCCTGGTGCT GGAGCGCAGC AGCACGCACT GGTGGTTAGC GGCACGGGCG 
CGCAGTGGCG AGACCGGGTA CGTGCCGCCT GCCTACCTGC ATCGCCTGCA GGGCATGGAG 
CAAGATGTCC TCCAAGCTAT TGACCGGGCC ATTGAGGCTG TGCACAACAC AGCCATGCGA 
GATGGTGGCA AGTACAGCCT GGAACAGCGT GGAGTCCTTC AGAAGCTTAT TCATCATCGG 
AAAGAGACCT TGTCTCGAAG GGGCACCTCA GCTGCCAGTG CTTCGGTTAT GACCCCATCC 
ACCAGTGACC ACCATTTGGA CACTGCTGTA TCTAGGCAGC CCAATGGGGT GTGTCGAACT 
GGGTTTGAAC GGCAGCATAG CCTACCCAGT TCTGAGCATC TCGGGACAGA TGGAGCCCTC 
TACCAGGTCC CACCACAGCC TCGGCGAGCA GCACCTGCCA CCCCACCCCC ACCAGTGAAG 
CGCCGAGACC GTGAGGCCCT GGTGATCTCA GGGAGTGGTG GCCGCACAGC AGTACCCTCT 
GGAGGTAGTT CTGTGTCTAG TGGCTCTTCA GTCAGCAGCA CCTCCATGGA CACACTCTAC 
ACTGGCTCTA GCCCCTCTGA GCTGGGTCCC AGCTGCTCAC CCACACCTCC GCCTGTACCT 
CGCCGAAGTG CCCACACTAC AGTGTCCCAG GCCCAGCCCT CTCCCTCCAA GGCACCATCC 
CCGGAGCCCC CTGCTGAGGA AGTGGCAGCT GAAACAAACT CAGCCCCTGA TGACCTCGAA 
GCTCTGGATG CCCTGAGCCC AGAGACCACA GAGGAGAAGC CAGCTACTGA GACAGTTGTG 
CCAAGGACCA TTGGGGCAGA GCTGATGGAA CTTGTTCGGA GAAACACTGG TCTGAGCCAC 
GAATTATGTC GTGTGGCCAT TGGTGTCGTG GTGGGTCACA TCCAGGCCTC CGTACCAGCC 
AGCTCGCCTG TCATGGAGCA GGTCCTCCTC TCGCTGGTAG AGGGCAAGGA CCTGAGCACA 
GCCCTACCCT CGGGGCAGGT CTGCCATGAC CAGCAGAGAC TGGAGGTGAT CTTTGCAGAC 
CTGGCTAGAA GGAAGGATGA CGCCCAGCAG CGCAGCTGGG CTCTTTACGA GGATGAGGAT 
GTCATCCGCT GCTACCTTGA GGAACTGTTG CACATCCTGA CGGATGCAGA TCCTGAAGTT 
TGCAAGAAAA TGTGCAAAAG GAGTGACTTC GAATCTGTCC TAGCCTTGGT GGCCTATTAC 
CAAATGGAAC ACCGAGCGTC ACTTCGGCTG CTGCTCCTCA AGTGCTTCGG CGCCATGTGC 
AGCCTGGACG CAGCAATCAT CTCTACTCTG GTCTCTTCTG TGCTACCTGT GGAATTGGCA 
CGGGACATGC AGACCGACAC ACAGGACCAC CAGAAGCTCT GTTATTCCGC CCTTGTCCTG 
GCCATGGTCT TCTCCATGGG AGAGGCGGTG CCCTACGCAC ACTATGAACA CCTGGGTACA 
CCCTTTGCCC AGTTCCTACT GAGCATCGTT GAGGATGGCC TGCCCCTGGA CGCTACAGAG 
CAGCTGCCAG ACCTCTGCAT GAACCTGCTT CTGGCTCTCA ACCTGCACCT GACAGCTCCT 
GACCAGAATG TCATCATGGC TGCCTTAAGC AAGCACACCA ACGTGAAGAT CTTCTCAGAG 
AAGCTGCTTT TGCTTCTGAA CAGAGGGGAC GATCCTGTGC GTATCTTCAG ACATGAGCCC 
CAGCCGCCAC ACTCTGTCCT CAAGTTCCTG CAGGACGTGT TCAGCAGCGC TGCCACGGCC 
TCCATCTTCT ACCACACAGA CATGATGGCG CTCATTGACA TCACTGTGCG CCAGATTGCC 
GACCTGTCAC CTGGAGACAA GCTACGCATG GAGTACCTCT CCCTGATGCA TGCCGTGGTC 
CGCTCCACAC CCTACCTCCA GCACCGGCAT CGGCTGTCTG ACCTGCAGGC TACACTGCGG 
CGCATCCTGA CTGAGGAAGA GACCTCACCC CAGTGCCAGA TGGACCGCAT GATTGTCCAA 
GAGATGTACA AGGAGTTTCC GGACCTGGGA GAGGTCCCCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPIN90 dephosphorylation is required for cofilin-mediated actin depolymerization in NMDA-stimulated hippocampal neurons.
Cellular and molecular life sciences : CMLS70(22)4369-83(2013 Nov)
Cho IH,Lee MJ,Kim DH,Kim B,Bae J,Choi KY,Kim SM,Huh YH,Lee KH,Kim CH,Song WK

Control of growth cone motility and neurite outgrowth by SPIN90.
Experimental cell research317(16)2276-87(2011 Oct)
Kim SM,Bae J,Cho IH,Choi KY,Park YJ,Ryu JH,Chun JS,Song WK

Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry.
Journal of proteome research7(11)4914-25(2008 Nov)
Fang L,Wang X,Yamoah K,Chen PL,Pan ZQ,Huang L

Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts.
Genes to cells : devoted to molecular & cellular mechanisms11(10)1197-211(2006 Oct)
Kim SH,Choi HJ,Lee KW,Hong NH,Sung BH,Choi KY,Kim SM,Chang S,Eom SH,Song WK

SPIN90/WISH interacts with PSD-95 and regulates dendritic spinogenesis via an N-WASP-independent mechanism.
The EMBO journal25(20)4983-95(2006 Oct)
Lee S,Lee K,Hwang S,Kim SH,Song WK,Park ZY,Chang S

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Nckipsd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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