Acy1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acy1
Entrez Gene ID	300981
Full Name	aminoacylase 1
Synonyms	Acy1a,Acy1b
General protein information	Preferred Names aminoacylase 1 Names aminoacylase-1A ACY IA ACY IB ACY-1A ACY-1B N-acyl-L-amino-acid amidohydrolase aminoacylase-1B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	enzyme that displays aminoacylase and/or metallopeptidase functions; may be involved in amino acid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005383.1	NP_001005383.1	aminoacylase-1A
XM_006243715.3	XP_006243777.1	aminoacylase-1A isoform X1
XM_006243716.3	XP_006243778.1	aminoacylase-1A isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG6738	NP_651124.1
Caenorhabditis elegans (roundworm)	C10C5.4	NP_501651.1
Mus musculus (house mouse)	Acy1	NP_079647.1
Drosophila melanogaster (fruit fly)	CG6733	NP_651123.1
Drosophila melanogaster (fruit fly)	CG17109	NP_651122.2
Caenorhabditis elegans (roundworm)	C06A6.4	NP_001021301.1
Caenorhabditis elegans (roundworm)	C10C5.3	NP_501650.1
Xenopus tropicalis (tropical clawed frog)	acy1.1	NP_001011406.1
Bos taurus (cattle)	ACY1	NP_001070583.1
Rattus norvegicus (Norway rat)	Acy1	NP_001005383.1
Gallus gallus (chicken)	ACY1	XP_004944531.1
Danio rerio (zebrafish)	acy1	NP_001259009.1
Drosophila melanogaster (fruit fly)	CG6465	NP_650004.1
Drosophila melanogaster (fruit fly)	CG6726	NP_732822.1
Caenorhabditis elegans (roundworm)	C10C5.5	NP_501652.2
Arabidopsis thaliana (thale cress)	AT4G38220	NP_849516.1
Homo sapiens (human)	ACY1	NP_000657.1
Canis lupus familiaris (dog)	ACY1	XP_533806.2
Drosophila melanogaster (fruit fly)	CG17110	NP_651121.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000679	XP_311208.4
Pan troglodytes (chimpanzee)	ACY1	XP_003950170.1
Danio rerio (zebrafish)	LOC100001620	XP_001922589.1

Gene Ontology
Bibliography

Related articles in PubMed

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
Zhang Y, Li Y, Qiu F, Qiu Z
Electrophoresis31(23-24)3797-807(2010 Dec)

Acylase 1 expression in rat intestinal crypt--villus axis.
Cigna N, Nicoletti C, Durand A, Chaix JC, Giardina T, Perrier J
Cell biology international31(9)966-73(2007 Sep)

The rat kidney acylase 1. Evidence for a new cDNA form and comparisons with the porcine intestinal enzyme.
Perrier J, Durand A, Giardina T, Puigserver A
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology138(3)277-83(2004 Jul)

Rat kidney acylase I: further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process.
Durand A, Giardina T, Villard C, Roussel A, Puigserver A, Perrier J
Biochimie85(10)953-62(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings proposed ACY 1 as a new marker transcript for absorbing cells of intestinal crypt, which can be used to monitor the process of intestinal N-alpha-acetylated protein metabolism.[ACY 1]
Title: Acylase 1 expression in rat intestinal crypt--villus axis.

active site characterization
Title: Rat kidney acylase I: further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process.

The following Acy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa31548	XM_006243715.3 Latest version!	Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa31548	XM_006243716.3 Latest version!	Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa10427	NM_001005383.1 Latest version!	Rattus norvegicus aminoacylase 1 (Acy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acy1 ( XM_006243715.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243777.1 Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31548
Clone ID Related Accession (Same CDS sequence)	XM_006243715.3 , XM_006243716.3
Accession Version	XM_006243715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aminoacylase-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCA AGGGTCCTGA GTCTGAGCAC CCCTCCGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGGGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA AGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243777.1
CDS	311..1537
Translation	MTTKGPESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFAQKFTEPRMTPTDDTDPWWAAFSGACKEMNLTLEPEIFPAATDSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPALPGES

Target ORF information:

RefSeq Version	XM_006243715.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243715.3

ORF Insert Sequence:

ATGACCACCA AGGGTCCTGA GTCTGAGCAC CCCTCCGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGGGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA AGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acy1 ( XM_006243716.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243778.1 Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31548
Clone ID Related Accession (Same CDS sequence)	XM_006243715.3 , XM_006243716.3
Accession Version	XM_006243716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aminoacylase-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCA AGGGTCCTGA GTCTGAGCAC CCCTCCGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGGGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA AGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243778.1
CDS	315..1541
Translation	MTTKGPESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFAQKFTEPRMTPTDDTDPWWAAFSGACKEMNLTLEPEIFPAATDSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPALPGES

Target ORF information:

RefSeq Version	XM_006243716.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoacylase 1 (Acy1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243716.3

ORF Insert Sequence:

ATGACCACCA AGGGTCCTGA GTCTGAGCAC CCCTCCGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGGGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA AGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acy1 ( NM_001005383.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005383.1 Rattus norvegicus aminoacylase 1 (Acy1), mRNA.

Price & Availability↑

CloneID	ORa10427
Clone ID Related Accession (Same CDS sequence)	NM_001005383.1
Accession Version	NM_001005383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	aminoacylase-1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY580164.1. On Oct 1, 2004 this sequence version replaced XM_217258.2. ##Evidence-Data-START## Transcript exon combination :: AY580164.1, AY580165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGACCAGCA AGGTTCCCGA GGAGGAGCAC CCATCGGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGAGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA GGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005383.1
CDS	1..1227
Translation	MTSKVPEEEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSESHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFAQKFTEPRMTPTDDTDPWWAAFSGACKEMNLTLEPEIFPAATDSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPALPGES

Target ORF information:

RefSeq Version	NM_001005383.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoacylase 1 (Acy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005383.1

ORF Insert Sequence:

ATGACCAGCA AGGTTCCCGA GGAGGAGCAC CCATCGGTGA CGCTCTTCCG CCAGTACCTG 
CGCATCTGCA CTGTGCAACC CAATCCTGAC TATGGGAGTG CGGTCACCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGTCT GAGCTGTCAG AAAATAGAGG TGGCGCCTGG CTATGTGATC 
ACTGTGCTGA CTTGGCCAGG CACCAACCCG TTGCTTCATT CCATCTTGCT AAACTCCCAC 
ACAGATGTGG TACCTGTCTT CAAGGAGCAT TGGCATCACG ACCCCTTTGA AGCCTTCAAG 
GATTCCGAGG GCTACATCTA TGCTAGGGGC GCCCAGGACA TGAAGAGTGT CAGCATCCAG 
TACCTGGAGG CCGTGAGAAG GCTGAAGAGC GAGAGCCACC GGTTCCCCAG AACCATCCAC 
ATGACATTTG TGCCTGACGA GGAGGTCGGA GGTCACAAGG GGATGGAGTT GTTTGTGAAG 
AGGCCTGAGT TCCAGGCTCT GCGGGCAGGC TTTGCTCTGG ATGAGGGCCT GGCCAACCCC 
ACTGATGCCT TCACCGTTTT TTATAGTGAA CGGAGTCCTT GGTGGATACG GGTTACCAGC 
ACTGGGAAGC CAGGCCATGC CTCACGCTTC ATTGAAGATA CAGCAGCTGA GAAGTTGCAT 
AAGGTTGTAA ACTCCATCTT GGCATTCCGG GAGAAGGAGA GGCAGAGGCT GCAGGCGAAC 
CCTCACCTGA AGGAAGGGGC CGTGACTTCG GTGAATCTGA CTAAGCTCGA GGGTGGTGTG 
GCCTATAATG TGGTACCTGC TACCATGAGT GCCTGTTTTG ACTTCCGAGT GGCGCCAGAT 
GTAGACATGA AGGCCTTCGA GAAGCAGCTG CAGAGCTGGT GCCAGGAAGC GGGAGAGGGG 
GTCACCTTTG AGTTTGCTCA GAAGTTTACA GAGCCTCGAA TGACACCCAC TGATGATACG 
GACCCCTGGT GGGCAGCTTT CAGCGGGGCC TGCAAGGAAA TGAACCTCAC TCTGGAGCCG 
GAGATCTTTC CTGCTGCCAC TGACAGCCGT TATATCCGAG CGGTGGGGAT CCCAGCTCTG 
GGCTTCTCGC CCATGAACCG CACACCCGTG TTACTACATG ACCATAACGA ACGACTGCAC 
GAGGCTGTGT TTCTACGTGG AGTTGACATA TATACACGCC TAGTTGCTGC ACTTGCTAGT 
GTGCCCGCTC TGCCTGGTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
Electrophoresis31(23-24)3797-807(2010 Dec)
Zhang Y,Li Y,Qiu F,Qiu Z

Acylase 1 expression in rat intestinal crypt--villus axis.
Cell biology international31(9)966-73(2007 Sep)
Cigna N,Nicoletti C,Durand A,Chaix JC,Giardina T,Perrier J

The rat kidney acylase 1. Evidence for a new cDNA form and comparisons with the porcine intestinal enzyme.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology138(3)277-83(2004 Jul)
Perrier J,Durand A,Giardina T,Puigserver A

Rat kidney acylase I: further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process.
Biochimie85(10)953-62(2003 Oct)
Durand A,Giardina T,Villard C,Roussel A,Puigserver A,Perrier J

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Acy1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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