Etfa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Etfa
Entrez Gene ID	300726
Full Name	electron transfer flavoprotein alpha subunit
Synonyms	ETF
General protein information	Preferred Names electron transfer flavoprotein alpha subunit Names electron transfer flavoprotein subunit alpha, mitochondrial alpha-ETF electron transferring flavoprotein, alpha polypeptide electron-transfer-flavoprotein, alpha polypeptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	specific electron acceptor for several dehydrogenases, including acyl-coA dehyrogenases, glutaryl-coA and sarcosine dehydrogenase; transfers electrons to the main mitochondrial respiratory chain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009668.1	NP_001009668.1	electron transfer flavoprotein subunit alpha, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Etfa	NP_663590.3
Danio rerio (zebrafish)	etfa	NP_944591.1
Caenorhabditis elegans (roundworm)	CELE_F27D4.1	NP_492146.1
Schizosaccharomyces pombe (fission yeast)	SPAC27D7.06	NP_594612.1
Arabidopsis thaliana (thale cress)	ETFALPHA	NP_175507.1
Homo sapiens (human)	ETFA	NP_000117.1
	ETFA	XP_001104838.1
Eremothecium gossypii	AGOS_AGL054W	NP_986612.1
Oryza sativa (rice)	Os03g0835400	NP_001051818.1
Canis lupus familiaris (dog)	ETFA	XP_853387.1
Rattus norvegicus (Norway rat)	Etfa	NP_001009668.1
Drosophila melanogaster (fruit fly)	wal	NP_476975.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004031	XP_318487.4
Kluyveromyces lactis	KLLA0E14037g	XP_454584.1
Neurospora crassa	NCU08004	XP_962674.2
Magnaporthe oryzae (rice blast fungus)	MGG_01719	XP_003714716.1
Xenopus tropicalis (tropical clawed frog)	etfa	NP_001016738.1
Pan troglodytes (chimpanzee)	ETFA	XP_510690.2
Bos taurus (cattle)	ETFA	NP_001069290.1
Gallus gallus (chicken)	ETFA	NP_001025714.1
Saccharomyces cerevisiae (baker's yeast)	AIM45	NP_015329.1

Gene Ontology
Bibliography

Related articles in PubMed

Human METTL20 is a mitochondrial lysine methyltransferase that targets the ? subunit of electron transfer flavoprotein (ETF?) and modulates its activity.
Ma?ecki J, Ho AY, Moen A, Dahl HA, Falnes P?
The Journal of biological chemistry290(1)423-34(2015 Jan)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Acyl-CoA dehydrogenases. A mechanistic overview.
Ghisla S, Thorpe C
European journal of biochemistry271(3)494-508(2004 Feb)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer.
Dwyer TM, Mortl S, Kemter K, Bacher A, Fauq A, Frerman FE
Biochemistry38(30)9735-45(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Etfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Etfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12462	NM_001009668.1 Latest version!	Rattus norvegicus electron transfer flavoprotein subunit alpha (Etfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Etfa ( NM_001009668.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009668.1 Rattus norvegicus electron transfer flavoprotein subunit alpha (Etfa), mRNA.

Price & Availability↑

CloneID	ORa12462
Clone ID Related Accession (Same CDS sequence)	NM_001009668.1
Accession Version	NM_001009668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	electron transfer flavoprotein subunit alpha, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088412.1. On Jan 13, 2005 this sequence version replaced XM_217155.2. ##Evidence-Data-START## Transcript exon combination :: BC088412.1, FQ219339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906356 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTTCGAG CAGCGGCGCC TGGGCAGCTC CGGCGGGCGG CCTCGTTGCT CCGTTTTCAG 
AGCACCTTGG TAATAGCTGA GCATGCGAAT GATTCTCTAG CACCCATTAC TCTAAACACT 
ATCACTGCAG CTGGACGTCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACAAAATGT 
GACAAGGTGG TACAGGATCT CTGTAAAGTA GCAGGCGTGG CAAAGGTTCT GGTGGCTCAG 
CATGATGCGT ACAAAGGCCT TCTTCCAGAG GAACTCACAC CATTGATTTT GGAAACTCAG 
AAGCAGTTCA GTTACACACA CATCTGTGCT GGAGCATCTG CTTTTGGAAA GAACCTTCTG 
CCCAGAGTAG CAGCCAAACT TAATGTTGCC CCAGTTTCTG ACATCATTGA GATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATCTATGCA GGAAATGCAT TGTGTACAGT GAAATGTGAT 
GAGAAAGTGA AGGTGTTTTC TGTTCGAGGA ACATCTTTTG AGGCTGCAGC AGCAAGTGGA 
GGTAGTGCCA GTTCAGAAAA GGCACCAAGT TCTTCATCAG CAGGAATATC AGAGTGGCTT 
GACCAGAAAT TGACAAAAAG TGACCGACCA GAGCTAACTG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGGG GCTTGAAGAG TGGAGAGAAC TTTAAACTGC TGTATGACTT GGCAGATCAG 
CTGCATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GTCAGACAGG AAAAATAGTA GCTCCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAGCATTT AGCTGGGATG AAGGACAGCA AGACAATTGT GGCTATTAAC 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTGGTTC CTGAAATGAC AGAAATACTG AAGAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009668.1
CDS	79..1080
Translation	MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKCDKVVQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLLPRVAAKLNVAPVSDIIEIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAAASGGSASSEKAPSSSSAGISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

Target ORF information:

RefSeq Version	NM_001009668.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus electron transfer flavoprotein subunit alpha (Etfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009668.1

ORF Insert Sequence:

ATGTTTCGAG CAGCGGCGCC TGGGCAGCTC CGGCGGGCGG CCTCGTTGCT CCGTTTTCAG 
AGCACCTTGG TAATAGCTGA GCATGCGAAT GATTCTCTAG CACCCATTAC TCTAAACACT 
ATCACTGCAG CTGGACGTCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACAAAATGT 
GACAAGGTGG TACAGGATCT CTGTAAAGTA GCAGGCGTGG CAAAGGTTCT GGTGGCTCAG 
CATGATGCGT ACAAAGGCCT TCTTCCAGAG GAACTCACAC CATTGATTTT GGAAACTCAG 
AAGCAGTTCA GTTACACACA CATCTGTGCT GGAGCATCTG CTTTTGGAAA GAACCTTCTG 
CCCAGAGTAG CAGCCAAACT TAATGTTGCC CCAGTTTCTG ACATCATTGA GATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATCTATGCA GGAAATGCAT TGTGTACAGT GAAATGTGAT 
GAGAAAGTGA AGGTGTTTTC TGTTCGAGGA ACATCTTTTG AGGCTGCAGC AGCAAGTGGA 
GGTAGTGCCA GTTCAGAAAA GGCACCAAGT TCTTCATCAG CAGGAATATC AGAGTGGCTT 
GACCAGAAAT TGACAAAAAG TGACCGACCA GAGCTAACTG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGGG GCTTGAAGAG TGGAGAGAAC TTTAAACTGC TGTATGACTT GGCAGATCAG 
CTGCATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GTCAGACAGG AAAAATAGTA GCTCCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAGCATTT AGCTGGGATG AAGGACAGCA AGACAATTGT GGCTATTAAC 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTGGTTC CTGAAATGAC AGAAATACTG AAGAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human METTL20 is a mitochondrial lysine methyltransferase that targets the ? subunit of electron transfer flavoprotein (ETF?) and modulates its activity.
The Journal of biological chemistry290(1)423-34(2015 Jan)
Ma?ecki J,Ho AY,Moen A,Dahl HA,Falnes P?

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

Acyl-CoA dehydrogenases. A mechanistic overview.
European journal of biochemistry271(3)494-508(2004 Feb)
Ghisla S,Thorpe C

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer.
Biochemistry38(30)9735-45(1999 Jul)
Dwyer TM,Mortl S,Kemter K,Bacher A,Fauq A,Frerman FE

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Etfa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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