Prkcsh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkcsh
Entrez Gene ID	300445
Full Name	protein kinase C substrate 80K-H
General protein information	Preferred Names protein kinase C substrate 80K-H Names glucosidase 2 subunit beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006242634.1	XP_006242696.1	glucosidase 2 subunit beta isoform X2
NM_001106806.2	NP_001100276.1	glucosidase 2 subunit beta precursor
XM_006242633.3	XP_006242695.1	glucosidase 2 subunit beta isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkcsh	NP_032951.1
Drosophila melanogaster (fruit fly)	CG6453	NP_001246063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004609	XP_315213.4
Xenopus tropicalis (tropical clawed frog)	prkcsh	NP_989356.1
Homo sapiens (human)	PRKCSH	NP_001001329.1
Pan troglodytes (chimpanzee)	PRKCSH	XP_003316136.2
Canis lupus familiaris (dog)	PRKCSH	XP_005632910.1
Caenorhabditis elegans (roundworm)	CELE_ZK1307.8	NP_496073.1
Danio rerio (zebrafish)	prkcsh	NP_957347.1
Bos taurus (cattle)	PRKCSH	NP_788835.1
Rattus norvegicus (Norway rat)	Prkcsh	NP_001100276.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of mammalian ER ?-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB, Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P, Zitzmann N
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)

Hepatocystin contributes to interferon-mediated antiviral response to hepatitis B virus by regulating hepatocyte nuclear factor 4?.
Shin GC, Ahn SH, Choi HS, Kim J, Park ES, Kim DH, Kim KH
Biochimica et biophysica acta1842(9)1648-57(2014 Sep)

TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.
Yaguchi H, Okumura F, Takahashi H, Kano T, Kameda H, Uchigashima M, Tanaka S, Watanabe M, Sasaki H, Hatakeyama S
The Journal of biological chemistry287(15)12050-9(2012 Apr)

A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation.
Fedeles SV, Tian X, Gallagher AR, Mitobe M, Nishio S, Lee SH, Cai Y, Geng L, Crews CM, Somlo S
Nature genetics43(7)639-47(2011 Jun)

PRKCSH/80K-H, the protein mutated in polycystic liver disease, protects polycystin-2/TRPP2 against HERP-mediated degradation.
Gao H, Wang Y, Wegierski T, Skouloudaki K, P?tz M, Fu X, Engel C, Boehlke C, Peng H, Kuehn EW, Kim E, Kramer-Zucker A, Walz G
Human molecular genetics19(1)16-24(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prkcsh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkcsh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02260	NM_001106806.2 Latest version!	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa02260	XM_006242633.3 Latest version!	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa30907	XM_006242634.1 Latest version!	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkcsh ( NM_001106806.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100276.1 Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), mRNA.

Price & Availability↑

CloneID	ORa02260
Clone ID Related Accession (Same CDS sequence)	NM_001106806.2 , XM_006242633.3
Accession Version	NM_001106806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-03
Organism	Rattus norvegicus(Norway rat)
Product	glucosidase 2 subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161987.1. On Apr 6, 2008 this sequence version replaced NM_001106806.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC161987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGT GCCGGGGGAG CAGCCCAAGG AGGTCCCGCC CCCACAGCAG 
CCCCTACGGC CTCCCAGTCC TGCTGAGGAT GAGAAGATGC CACCCTATGA TGAGGAGACA 
CAGGCTATCA TCGATGCCGC ACAAGAGGCC CGGAACAAGT TTGAGGAGGT CGAACGGTCC 
TTGAAAGAGA TGGAGGAGTC CATCAGGAGT TTGGAACAAG AGATCTCCTT TGATTTCGGT 
CCCTCTGGAG AGTTTGCATA CCTCTACAGC CAATGCTACG AGCTCACCAC CAATGAGTAT 
GTCTACCGGC TTTGCCCCTT CAAACTTGTC TCCCAGAAAC CCAAACATGG GGGCTCCCCG 
ACCAGTCTGG GTACATGGGG CTCCTGGGCT GGCCCAGATG ACGACAAGTT CAGCGCAATG 
AAGTACGAGC AGGGCACAGG CTGCTGGCAG GGCCCCAACC GCTCCACCAC AGTACGCCTC 
CTGTGTGGCA AGGAGACTGT GGTGACCAGT ACCACAGAGC CCAGTCGCTG CGAGTACCTC 
ATGGAGCTGA TGACACCAGC AGCCTGCCCA GAGCCGCCTC CAGAAGTACC CACTGATGGA 
GACCATGATG AACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100276.1
CDS	84..1661
Translation	MLLLLLLLPLCWAVEVKRPRGVSLSNHHFYDESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRTAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQELAASAFQELDDNMDGLVSLAELQTHPELDTDGDGGLSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEVTEPKEEKLPVLPPMEEEEEEEEPEEEEEEEEEEVPGEQPKEVPPPQQPLRPPSPAEDEKMPPYDEETQAIIDAAQEARNKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDDDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEVPTDGDHDEL

Target ORF information:

RefSeq Version	NM_001106806.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106806.2

ORF Insert Sequence:

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGT GCCGGGGGAG CAGCCCAAGG AGGTCCCGCC CCCACAGCAG 
CCCCTACGGC CTCCCAGTCC TGCTGAGGAT GAGAAGATGC CACCCTATGA TGAGGAGACA 
CAGGCTATCA TCGATGCCGC ACAAGAGGCC CGGAACAAGT TTGAGGAGGT CGAACGGTCC 
TTGAAAGAGA TGGAGGAGTC CATCAGGAGT TTGGAACAAG AGATCTCCTT TGATTTCGGT 
CCCTCTGGAG AGTTTGCATA CCTCTACAGC CAATGCTACG AGCTCACCAC CAATGAGTAT 
GTCTACCGGC TTTGCCCCTT CAAACTTGTC TCCCAGAAAC CCAAACATGG GGGCTCCCCG 
ACCAGTCTGG GTACATGGGG CTCCTGGGCT GGCCCAGATG ACGACAAGTT CAGCGCAATG 
AAGTACGAGC AGGGCACAGG CTGCTGGCAG GGCCCCAACC GCTCCACCAC AGTACGCCTC 
CTGTGTGGCA AGGAGACTGT GGTGACCAGT ACCACAGAGC CCAGTCGCTG CGAGTACCTC 
ATGGAGCTGA TGACACCAGC AGCCTGCCCA GAGCCGCCTC CAGAAGTACC CACTGATGGA 
GACCATGATG AACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( XM_006242633.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242695.1 Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02260
Clone ID Related Accession (Same CDS sequence)	NM_001106806.2 , XM_006242633.3
Accession Version	XM_006242633.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucosidase 2 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGT GCCGGGGGAG CAGCCCAAGG AGGTCCCGCC CCCACAGCAG 
CCCCTACGGC CTCCCAGTCC TGCTGAGGAT GAGAAGATGC CACCCTATGA TGAGGAGACA 
CAGGCTATCA TCGATGCCGC ACAAGAGGCC CGGAACAAGT TTGAGGAGGT CGAACGGTCC 
TTGAAAGAGA TGGAGGAGTC CATCAGGAGT TTGGAACAAG AGATCTCCTT TGATTTCGGT 
CCCTCTGGAG AGTTTGCATA CCTCTACAGC CAATGCTACG AGCTCACCAC CAATGAGTAT 
GTCTACCGGC TTTGCCCCTT CAAACTTGTC TCCCAGAAAC CCAAACATGG GGGCTCCCCG 
ACCAGTCTGG GTACATGGGG CTCCTGGGCT GGCCCAGATG ACGACAAGTT CAGCGCAATG 
AAGTACGAGC AGGGCACAGG CTGCTGGCAG GGCCCCAACC GCTCCACCAC AGTACGCCTC 
CTGTGTGGCA AGGAGACTGT GGTGACCAGT ACCACAGAGC CCAGTCGCTG CGAGTACCTC 
ATGGAGCTGA TGACACCAGC AGCCTGCCCA GAGCCGCCTC CAGAAGTACC CACTGATGGA 
GACCATGATG AACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242695.1
CDS	151..1728
Translation	MLLLLLLLPLCWAVEVKRPRGVSLSNHHFYDESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRTAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQELAASAFQELDDNMDGLVSLAELQTHPELDTDGDGGLSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEVTEPKEEKLPVLPPMEEEEEEEEPEEEEEEEEEEVPGEQPKEVPPPQQPLRPPSPAEDEKMPPYDEETQAIIDAAQEARNKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDDDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEVPTDGDHDEL

Target ORF information:

RefSeq Version	XM_006242633.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242633.3

ORF Insert Sequence:

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGT GCCGGGGGAG CAGCCCAAGG AGGTCCCGCC CCCACAGCAG 
CCCCTACGGC CTCCCAGTCC TGCTGAGGAT GAGAAGATGC CACCCTATGA TGAGGAGACA 
CAGGCTATCA TCGATGCCGC ACAAGAGGCC CGGAACAAGT TTGAGGAGGT CGAACGGTCC 
TTGAAAGAGA TGGAGGAGTC CATCAGGAGT TTGGAACAAG AGATCTCCTT TGATTTCGGT 
CCCTCTGGAG AGTTTGCATA CCTCTACAGC CAATGCTACG AGCTCACCAC CAATGAGTAT 
GTCTACCGGC TTTGCCCCTT CAAACTTGTC TCCCAGAAAC CCAAACATGG GGGCTCCCCG 
ACCAGTCTGG GTACATGGGG CTCCTGGGCT GGCCCAGATG ACGACAAGTT CAGCGCAATG 
AAGTACGAGC AGGGCACAGG CTGCTGGCAG GGCCCCAACC GCTCCACCAC AGTACGCCTC 
CTGTGTGGCA AGGAGACTGT GGTGACCAGT ACCACAGAGC CCAGTCGCTG CGAGTACCTC 
ATGGAGCTGA TGACACCAGC AGCCTGCCCA GAGCCGCCTC CAGAAGTACC CACTGATGGA 
GACCATGATG AACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( XM_006242634.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242696.1 Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30907
Clone ID Related Accession (Same CDS sequence)	XM_006242634.1
Accession Version	XM_006242634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucosidase 2 subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGA GGTCCCGCCC CCACAGCAGC CCCTACGGCC TCCCAGTCCT 
GCTGAGGATG AGAAGATGCC ACCCTATGAT GAGGAGACAC AGGCTATCAT CGATGCCGCA 
CAAGAGGCCC GGAACAAGTT TGAGGAGGTC GAACGGTCCT TGAAAGAGAT GGAGGAGTCC 
ATCAGGAGTT TGGAACAAGA GATCTCCTTT GATTTCGGTC CCTCTGGAGA GTTTGCATAC 
CTCTACAGCC AATGCTACGA GCTCACCACC AATGAGTATG TCTACCGGCT TTGCCCCTTC 
AAACTTGTCT CCCAGAAACC CAAACATGGG GGCTCCCCGA CCAGTCTGGG TACATGGGGC 
TCCTGGGCTG GCCCAGATGA CGACAAGTTC AGCGCAATGA AGTACGAGCA GGGCACAGGC 
TGCTGGCAGG GCCCCAACCG CTCCACCACA GTACGCCTCC TGTGTGGCAA GGAGACTGTG 
GTGACCAGTA CCACAGAGCC CAGTCGCTGC GAGTACCTCA TGGAGCTGAT GACACCAGCA 
GCCTGCCCAG AGCCGCCTCC AGAAGTACCC ACTGATGGAG ACCATGATGA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242696.1
CDS	238..1794
Translation	MLLLLLLLPLCWAVEVKRPRGVSLSNHHFYDESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRTAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQELAASAFQELDDNMDGLVSLAELQTHPELDTDGDGGLSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEVTEPKEEKLPVLPPMEEEEEEEEPEEEEEEEEEEEVPPPQQPLRPPSPAEDEKMPPYDEETQAIIDAAQEARNKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDDDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEVPTDGDHDEL

Target ORF information:

RefSeq Version	XM_006242634.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242634.1

ORF Insert Sequence:

ATGTTGCTGC TGCTGCTGCT GCTACCCCTC TGTTGGGCAG TAGAAGTTAA GAGACCCCGG 
GGCGTTTCCC TCAGCAACCA TCACTTCTAT GACGAATCTA AACCTTTCAC GTGTTTGGAT 
GGCACAGCCA CTATCCCCTT CGATCAGGTG AACGATGATT ACTGCGACTG TAAGGATGGT 
TCTGATGAGC CTGGCACAGC TGCGTGTCCC AATGGCAGCT TTCACTGCAC CAACACTGGG 
TATAAGCCTT TGTACATCCT CTCTAGCCGG GTCAATGATG GGGTGTGTGA CTGCTGTGAT 
GGGACGGACG AGTACAACAG TGGCACCGTC TGTGAGAACA CCTGCAGAGA GAAGGGTCGC 
AAGGAAAAAG AGTCCCTGCA GCAGCTGGCC GAGGTCACCC GAGAAGGGTT CCGTCTCAAG 
AAGATACTCA TTGAGGAGTG GAAGACAGCT CGGGAAGAAA AGCAGAGTAA GCTTCTTGAG 
CTTCAGGCTG GAAAGAAGTC TCTGGAAGAC CAGGTAGAAA CGCTGCGGAC AGCGAAAGAG 
GAAGCAGAGA GGCCAGAGAA GGAGGCCAAG GACCAGCACC GGAAGCTGTG GGAAGAGCAG 
CAGGCTGCTG CCAAGGCCCG GCGGGAACAG GAGCTGGCAG CCAGTGCCTT CCAGGAACTG 
GACGACAACA TGGATGGGCT GGTCTCACTG GCTGAGTTAC AGACTCACCC AGAGCTGGAC 
ACAGATGGAG ATGGAGGACT GTCGGAGGAG GAGGCCCAGG CTCTTCTCAG TGGAGACACA 
CAGACTGACA CTACCTCCTT CTATGACCGT GTCTGGGCTG CCATCAGGGA CAAGTACCGC 
TCTGAGGTCC CACCCACGGA CATACCTGTT CCTGAGGTCA CCGAACCCAA AGAAGAAAAG 
CTGCCAGTGT TGCCCCCCAT GGAAGAGGAG GAGGAAGAGG AGGAGCCAGA GGAGGAAGAG 
GAGGAGGAGG AGGAGGAGGA GGTCCCGCCC CCACAGCAGC CCCTACGGCC TCCCAGTCCT 
GCTGAGGATG AGAAGATGCC ACCCTATGAT GAGGAGACAC AGGCTATCAT CGATGCCGCA 
CAAGAGGCCC GGAACAAGTT TGAGGAGGTC GAACGGTCCT TGAAAGAGAT GGAGGAGTCC 
ATCAGGAGTT TGGAACAAGA GATCTCCTTT GATTTCGGTC CCTCTGGAGA GTTTGCATAC 
CTCTACAGCC AATGCTACGA GCTCACCACC AATGAGTATG TCTACCGGCT TTGCCCCTTC 
AAACTTGTCT CCCAGAAACC CAAACATGGG GGCTCCCCGA CCAGTCTGGG TACATGGGGC 
TCCTGGGCTG GCCCAGATGA CGACAAGTTC AGCGCAATGA AGTACGAGCA GGGCACAGGC 
TGCTGGCAGG GCCCCAACCG CTCCACCACA GTACGCCTCC TGTGTGGCAA GGAGACTGTG 
GTGACCAGTA CCACAGAGCC CAGTCGCTGC GAGTACCTCA TGGAGCTGAT GACACCAGCA 
GCCTGCCCAG AGCCGCCTCC AGAAGTACCC ACTGATGGAG ACCATGATGA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of mammalian ER ?-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)
Caputo AT,Alonzi DS,Marti L,Reca IB,Kiappes JL,Struwe WB,Cross A,Basu S,Lowe ED,Darlot B,Santino A,Roversi P,Zitzmann N

Hepatocystin contributes to interferon-mediated antiviral response to hepatitis B virus by regulating hepatocyte nuclear factor 4?.
Biochimica et biophysica acta1842(9)1648-57(2014 Sep)
Shin GC,Ahn SH,Choi HS,Kim J,Park ES,Kim DH,Kim KH

TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.
The Journal of biological chemistry287(15)12050-9(2012 Apr)
Yaguchi H,Okumura F,Takahashi H,Kano T,Kameda H,Uchigashima M,Tanaka S,Watanabe M,Sasaki H,Hatakeyama S

A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation.
Nature genetics43(7)639-47(2011 Jun)
Fedeles SV,Tian X,Gallagher AR,Mitobe M,Nishio S,Lee SH,Cai Y,Geng L,Crews CM,Somlo S

PRKCSH/80K-H, the protein mutated in polycystic liver disease, protects polycystin-2/TRPP2 against HERP-mediated degradation.
Human molecular genetics19(1)16-24(2010 Jan)
Gao H,Wang Y,Wegierski T,Skouloudaki K,P?tz M,Fu X,Engel C,Boehlke C,Peng H,Kuehn EW,Kim E,Kramer-Zucker A,Walz G

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Prkcsh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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