Ddx23 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx23
Entrez Gene ID	300208
Full Name	DEAD-box helicase 23
General protein information	Preferred Names DEAD-box helicase 23 Names probable ATP-dependent RNA helicase DDX23 DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106793.2	NP_001100263.2	probable ATP-dependent RNA helicase DDX23
XM_006257343.3	XP_006257405.1	probable ATP-dependent RNA helicase DDX23 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX23	NP_004809.2
Gallus gallus (chicken)	DDX23	XP_003643496.2
Danio rerio (zebrafish)	ddx23	NP_956176.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007825	XP_317676.4
	DDX23	XP_001104339.1
Mus musculus (house mouse)	Ddx23	NP_001074450.1
Canis lupus familiaris (dog)	DDX23	XP_851051.1
Schizosaccharomyces pombe (fission yeast)	prp28	NP_587984.1
Magnaporthe oryzae (rice blast fungus)	MGG_01583	XP_003714561.1
Oryza sativa (rice)	Os03g0708600	NP_001051038.1
Rattus norvegicus (Norway rat)	Ddx23	NP_001100263.2
Drosophila melanogaster (fruit fly)	CG10333	NP_609888.2
Caenorhabditis elegans (roundworm)	ddx-23	NP_498260.2
Neurospora crassa	NCU02803	XP_964459.1
Arabidopsis thaliana (thale cress)	AT2G33730	NP_180929.1
Pan troglodytes (chimpanzee)	DDX23	XP_509035.2
Bos taurus (cattle)	DDX23	NP_001095672.1

Gene Ontology
Bibliography

Related articles in PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Chang TH, Tung L, Yeh FL, Chen JH, Chang SL
Biochimica et biophysica acta1829(8)764-74(2013 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

The spliceosome: design principles of a dynamic RNP machine.
Wahl MC, Will CL, L?hrmann R
Cell136(4)701-18(2009 Feb)

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ
RNA (New York, N.Y.)8(4)426-39(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02227	NM_001106793.2 Latest version!	Rattus norvegicus DEAD-box helicase 23 (Ddx23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa02227	XM_006257343.3 Latest version!	Rattus norvegicus DEAD-box helicase 23 (Ddx23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx23 ( NM_001106793.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100263.2 Rattus norvegicus DEAD-box helicase 23 (Ddx23), mRNA.

Price & Availability↑

CloneID	ORa02227
Clone ID Related Accession (Same CDS sequence)	NM_001106793.2 , XM_006257343.3
Accession Version	NM_001106793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX23
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB612721.1, BC169082.1 and CO570626.1. On Oct 15, 2009 this sequence version replaced NM_001106793.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGAG AACTGGCTGA CAAGAAGGAC CGAGACGCAT CACCCAAGGA AGAAAGGAAG 
CGATCGCGGA CTCCTGAAAG AGATCGCGAT AGAGACCGGG ACCGGAAGTC CTCCCCATCT 
AAAGACCGGA AGAGGCATCG CTCACGGGAT AGGCGTCGAG GAGGTAGTCG TTCTCGCTCT 
CGGTCCCGTT CCAAGTCTAC AGAGAGGGAA CGACGGCACA AAGAACGGGA ACGAGATAAA 
GAACGGGATC GGAGTAAGAA GGAGCGGGAG CGGGACAAGG ATGGCCACCG GCGGGACAAG 
GACCGCAAGA GATCCAGCTT GTCTCCTGGT CGAGGAAAAG ATTTCAAGTC TCGGAAGGAC 
AGAGATTCTA AGAAGGGTGA CGAGGATGAG CATGGTGACA AGAGGCCTAA GATCCAGCCA 
CTGTCCCTGG AGGAACTTCT GGCCAAGAAA AAGGCTGAGG AAGAAGCTGA AGCTAAGCCT 
AAATTCCTCT CCAAAGCAGA GCGAGAGGCT GAAGCTCTGA AGCGGCGGCA GCAGGAGGTG 
GAGGAGCGAC AGAAGATGCT CGAAGAAGAG AGGAAGAAGA GGAAACAGTT CCAGGATTTG 
GGCAGGAAGA TGCTGGAAGA CCCTCAGGAA AGGGAACGGA GGGAACGCAG GGAAAGGATG 
GAGCGAGAGA CCAATGGCAA CGAGGATGAG GAGGGCCGGC AGAAGATCCG GGAGGAGAAG 
GACAAGAGCA AGGAGCTGCA CGCTATTAAG GAGCGTTACC TGGGTGGCAT CAAAAAACGG 
CGCCGGACAA GGCATCTCAA TGACCGCAAG TTTGTGTTTG AGTGGGATGC GTCTGAAGAC 
ACCTCCATTG ACTACAACCC CCTGTACAAA GAACGGCACC AGGTGCAGCT GTTGGGGCGG 
GGCTTCATTG CGGGCATTGA CCTAAAGCAG CAGAAGCGAG AACAGTCACG TTTCTATGGA 
GACCTAATGG AAAAGAGACG AACCCTGGAA GAAAAGGAGC AGGAGGAAGC AAGACTCCGC 
AAACTTCGTA AGAAGGAAGC CAAGCAACGC TGGGATGATC GCCACTGGTC CCAGAAGAAG 
TTAGATGAGA TGACAGACAG GGACTGGCGG ATCTTTCGGG AAGACTATAG CATCACCACC 
AAAGGTGGCA AGATTCCCAA TCCTATTCGG TCCTGGAAAG ATTCGTCTCT GCCCCCACAT 
ATCTTGGAAG TCATTGATAA GTGTGGCTAC AAGGAGCCAA CACCTATCCA GCGTCAGGCC 
ATTCCCATTG GGCTTCAGAA TCGTGACATC ATTGGCGTGG CTGAGACTGG CAGTGGCAAG 
ACAGCAGCCT TCCTCATCCC ATTACTGGTC TGGATCACCA CACTTCCCAA AATTGACAGG 
ATCGAAGAGT CCGACCAGGG CCCTTATGCC ATCATCCTGG CTCCCACCCG TGAATTGGCT 
CAGCAGATCG AGGAAGAGAC CATCAAGTTC GGAAAGCCAC TAGGTATCCG CACTGTGGCT 
GTCATTGGTG GCATCTCCAG AGAGGACCAA GGCTTCAGGC TACGCATGGG TTGTGAGATT 
GTGATCGCCA CCCCAGGACG TCTGATTGAC GTGCTGGAGA ACCGTTACCT GGTGCTCAGC 
CGCTGCACCT ATGTGGTTCT GGATGAGGCC GACAGGATGA TTGACATGGG CTTCGAGCCA 
GATGTGCAGA AGATCCTGGA GCACATGCCT GTCAGCAACC AGAAGCCGGA CACGGACGAG 
GCCGAGGACC CCGAGAAGAT GTTAGCCAAC TTTGAATCAG GAAAACACAA GTACCGTCAA 
ACAGTCATGT TCACGGCTAC CATGCCTCCA GCGGTGGAGC GTCTAGCCAG GAGCTATCTT 
CGGCGGCCTG CTGTGGTGTA CATTGGCTCT GCAGGCAAGC CCCATGAGCG GGTGGAACAG 
AAGGTCTTTC TCATGTCGGA GTCAGAAAAG AGGAAAAAGC TGCTGGCAAT CCTGGAACAA 
GGTTTTGATC CACCCATCAT CATCTTTGTC AACCAGAAGA AGGGTTGCGA CGTGTTGGCC 
AAGTCTCTGG AGAAGATGGG GTACAACGCC TGTACACTGC ATGGTGGCAA AGGCCAGGAG 
CAGCGAGAAT TCGCATTGTC CAACCTCAAG GCTGGAGCCA AGGATATTCT GGTGGCTACA 
GATGTGGCTG GTCGTGGTAT CGATATCCAA GACGTATCTA TGGTTGTCAA CTACGACATG 
GCCAAAAACA TTGAAGATTA CATTCACCGA ATTGGCCGCA CAGGAAGAGC AGGCAAGAGT 
GGTGTAGCCA TCACCTTCCT CACCAAAGAG GACTCAGCCG TGTTCTATGA ACTGAAGCAA 
GCCATCCTGG AGAGCCCAGT GTCCTCTTGC CCTCCAGAGC TGGCAAACCA CCCAGATGCC 
CAGCACAAGC CAGGCACCAT CCTTACCAAG AAGCGCAGGG AAGAGACCAT CTTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100263.2
CDS	60..2519
Translation	MAGELADKKDRDASPKEERKRSRTPERDRDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSTERERRHKERERDKERDRSKKERERDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKGDEDEHGDKRPKIQPLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQKMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA

Target ORF information:

RefSeq Version	NM_001106793.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 23 (Ddx23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106793.2

ORF Insert Sequence:

ATGGCAGGAG AACTGGCTGA CAAGAAGGAC CGAGACGCAT CACCCAAGGA AGAAAGGAAG 
CGATCGCGGA CTCCTGAAAG AGATCGCGAT AGAGACCGGG ACCGGAAGTC CTCCCCATCT 
AAAGACCGGA AGAGGCATCG CTCACGGGAT AGGCGTCGAG GAGGTAGTCG TTCTCGCTCT 
CGGTCCCGTT CCAAGTCTAC AGAGAGGGAA CGACGGCACA AAGAACGGGA ACGAGATAAA 
GAACGGGATC GGAGTAAGAA GGAGCGGGAG CGGGACAAGG ATGGCCACCG GCGGGACAAG 
GACCGCAAGA GATCCAGCTT GTCTCCTGGT CGAGGAAAAG ATTTCAAGTC TCGGAAGGAC 
AGAGATTCTA AGAAGGGTGA CGAGGATGAG CATGGTGACA AGAGGCCTAA GATCCAGCCA 
CTGTCCCTGG AGGAACTTCT GGCCAAGAAA AAGGCTGAGG AAGAAGCTGA AGCTAAGCCT 
AAATTCCTCT CCAAAGCAGA GCGAGAGGCT GAAGCTCTGA AGCGGCGGCA GCAGGAGGTG 
GAGGAGCGAC AGAAGATGCT CGAAGAAGAG AGGAAGAAGA GGAAACAGTT CCAGGATTTG 
GGCAGGAAGA TGCTGGAAGA CCCTCAGGAA AGGGAACGGA GGGAACGCAG GGAAAGGATG 
GAGCGAGAGA CCAATGGCAA CGAGGATGAG GAGGGCCGGC AGAAGATCCG GGAGGAGAAG 
GACAAGAGCA AGGAGCTGCA CGCTATTAAG GAGCGTTACC TGGGTGGCAT CAAAAAACGG 
CGCCGGACAA GGCATCTCAA TGACCGCAAG TTTGTGTTTG AGTGGGATGC GTCTGAAGAC 
ACCTCCATTG ACTACAACCC CCTGTACAAA GAACGGCACC AGGTGCAGCT GTTGGGGCGG 
GGCTTCATTG CGGGCATTGA CCTAAAGCAG CAGAAGCGAG AACAGTCACG TTTCTATGGA 
GACCTAATGG AAAAGAGACG AACCCTGGAA GAAAAGGAGC AGGAGGAAGC AAGACTCCGC 
AAACTTCGTA AGAAGGAAGC CAAGCAACGC TGGGATGATC GCCACTGGTC CCAGAAGAAG 
TTAGATGAGA TGACAGACAG GGACTGGCGG ATCTTTCGGG AAGACTATAG CATCACCACC 
AAAGGTGGCA AGATTCCCAA TCCTATTCGG TCCTGGAAAG ATTCGTCTCT GCCCCCACAT 
ATCTTGGAAG TCATTGATAA GTGTGGCTAC AAGGAGCCAA CACCTATCCA GCGTCAGGCC 
ATTCCCATTG GGCTTCAGAA TCGTGACATC ATTGGCGTGG CTGAGACTGG CAGTGGCAAG 
ACAGCAGCCT TCCTCATCCC ATTACTGGTC TGGATCACCA CACTTCCCAA AATTGACAGG 
ATCGAAGAGT CCGACCAGGG CCCTTATGCC ATCATCCTGG CTCCCACCCG TGAATTGGCT 
CAGCAGATCG AGGAAGAGAC CATCAAGTTC GGAAAGCCAC TAGGTATCCG CACTGTGGCT 
GTCATTGGTG GCATCTCCAG AGAGGACCAA GGCTTCAGGC TACGCATGGG TTGTGAGATT 
GTGATCGCCA CCCCAGGACG TCTGATTGAC GTGCTGGAGA ACCGTTACCT GGTGCTCAGC 
CGCTGCACCT ATGTGGTTCT GGATGAGGCC GACAGGATGA TTGACATGGG CTTCGAGCCA 
GATGTGCAGA AGATCCTGGA GCACATGCCT GTCAGCAACC AGAAGCCGGA CACGGACGAG 
GCCGAGGACC CCGAGAAGAT GTTAGCCAAC TTTGAATCAG GAAAACACAA GTACCGTCAA 
ACAGTCATGT TCACGGCTAC CATGCCTCCA GCGGTGGAGC GTCTAGCCAG GAGCTATCTT 
CGGCGGCCTG CTGTGGTGTA CATTGGCTCT GCAGGCAAGC CCCATGAGCG GGTGGAACAG 
AAGGTCTTTC TCATGTCGGA GTCAGAAAAG AGGAAAAAGC TGCTGGCAAT CCTGGAACAA 
GGTTTTGATC CACCCATCAT CATCTTTGTC AACCAGAAGA AGGGTTGCGA CGTGTTGGCC 
AAGTCTCTGG AGAAGATGGG GTACAACGCC TGTACACTGC ATGGTGGCAA AGGCCAGGAG 
CAGCGAGAAT TCGCATTGTC CAACCTCAAG GCTGGAGCCA AGGATATTCT GGTGGCTACA 
GATGTGGCTG GTCGTGGTAT CGATATCCAA GACGTATCTA TGGTTGTCAA CTACGACATG 
GCCAAAAACA TTGAAGATTA CATTCACCGA ATTGGCCGCA CAGGAAGAGC AGGCAAGAGT 
GGTGTAGCCA TCACCTTCCT CACCAAAGAG GACTCAGCCG TGTTCTATGA ACTGAAGCAA 
GCCATCCTGG AGAGCCCAGT GTCCTCTTGC CCTCCAGAGC TGGCAAACCA CCCAGATGCC 
CAGCACAAGC CAGGCACCAT CCTTACCAAG AAGCGCAGGG AAGAGACCAT CTTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx23 ( XM_006257343.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257405.1 Rattus norvegicus DEAD-box helicase 23 (Ddx23), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02227
Clone ID Related Accession (Same CDS sequence)	NM_001106793.2 , XM_006257343.3
Accession Version	XM_006257343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAG AACTGGCTGA CAAGAAGGAC CGAGACGCAT CACCCAAGGA AGAAAGGAAG 
CGATCGCGGA CTCCTGAAAG AGATCGCGAT AGAGACCGGG ACCGGAAGTC CTCCCCATCT 
AAAGACCGGA AGAGGCATCG CTCACGGGAT AGGCGTCGAG GAGGTAGTCG TTCTCGCTCT 
CGGTCCCGTT CCAAGTCTAC AGAGAGGGAA CGACGGCACA AAGAACGGGA ACGAGATAAA 
GAACGGGATC GGAGTAAGAA GGAGCGGGAG CGGGACAAGG ATGGCCACCG GCGGGACAAG 
GACCGCAAGA GATCCAGCTT GTCTCCTGGT CGAGGAAAAG ATTTCAAGTC TCGGAAGGAC 
AGAGATTCTA AGAAGGGTGA CGAGGATGAG CATGGTGACA AGAGGCCTAA GATCCAGCCA 
CTGTCCCTGG AGGAACTTCT GGCCAAGAAA AAGGCTGAGG AAGAAGCTGA AGCTAAGCCT 
AAATTCCTCT CCAAAGCAGA GCGAGAGGCT GAAGCTCTGA AGCGGCGGCA GCAGGAGGTG 
GAGGAGCGAC AGAAGATGCT CGAAGAAGAG AGGAAGAAGA GGAAACAGTT CCAGGATTTG 
GGCAGGAAGA TGCTGGAAGA CCCTCAGGAA AGGGAACGGA GGGAACGCAG GGAAAGGATG 
GAGCGAGAGA CCAATGGCAA CGAGGATGAG GAGGGCCGGC AGAAGATCCG GGAGGAGAAG 
GACAAGAGCA AGGAGCTGCA CGCTATTAAG GAGCGTTACC TGGGTGGCAT CAAAAAACGG 
CGCCGGACAA GGCATCTCAA TGACCGCAAG TTTGTGTTTG AGTGGGATGC GTCTGAAGAC 
ACCTCCATTG ACTACAACCC CCTGTACAAA GAACGGCACC AGGTGCAGCT GTTGGGGCGG 
GGCTTCATTG CGGGCATTGA CCTAAAGCAG CAGAAGCGAG AACAGTCACG TTTCTATGGA 
GACCTAATGG AAAAGAGACG AACCCTGGAA GAAAAGGAGC AGGAGGAAGC AAGACTCCGC 
AAACTTCGTA AGAAGGAAGC CAAGCAACGC TGGGATGATC GCCACTGGTC CCAGAAGAAG 
TTAGATGAGA TGACAGACAG GGACTGGCGG ATCTTTCGGG AAGACTATAG CATCACCACC 
AAAGGTGGCA AGATTCCCAA TCCTATTCGG TCCTGGAAAG ATTCGTCTCT GCCCCCACAT 
ATCTTGGAAG TCATTGATAA GTGTGGCTAC AAGGAGCCAA CACCTATCCA GCGTCAGGCC 
ATTCCCATTG GGCTTCAGAA TCGTGACATC ATTGGCGTGG CTGAGACTGG CAGTGGCAAG 
ACAGCAGCCT TCCTCATCCC ATTACTGGTC TGGATCACCA CACTTCCCAA AATTGACAGG 
ATCGAAGAGT CCGACCAGGG CCCTTATGCC ATCATCCTGG CTCCCACCCG TGAATTGGCT 
CAGCAGATCG AGGAAGAGAC CATCAAGTTC GGAAAGCCAC TAGGTATCCG CACTGTGGCT 
GTCATTGGTG GCATCTCCAG AGAGGACCAA GGCTTCAGGC TACGCATGGG TTGTGAGATT 
GTGATCGCCA CCCCAGGACG TCTGATTGAC GTGCTGGAGA ACCGTTACCT GGTGCTCAGC 
CGCTGCACCT ATGTGGTTCT GGATGAGGCC GACAGGATGA TTGACATGGG CTTCGAGCCA 
GATGTGCAGA AGATCCTGGA GCACATGCCT GTCAGCAACC AGAAGCCGGA CACGGACGAG 
GCCGAGGACC CCGAGAAGAT GTTAGCCAAC TTTGAATCAG GAAAACACAA GTACCGTCAA 
ACAGTCATGT TCACGGCTAC CATGCCTCCA GCGGTGGAGC GTCTAGCCAG GAGCTATCTT 
CGGCGGCCTG CTGTGGTGTA CATTGGCTCT GCAGGCAAGC CCCATGAGCG GGTGGAACAG 
AAGGTCTTTC TCATGTCGGA GTCAGAAAAG AGGAAAAAGC TGCTGGCAAT CCTGGAACAA 
GGTTTTGATC CACCCATCAT CATCTTTGTC AACCAGAAGA AGGGTTGCGA CGTGTTGGCC 
AAGTCTCTGG AGAAGATGGG GTACAACGCC TGTACACTGC ATGGTGGCAA AGGCCAGGAG 
CAGCGAGAAT TCGCATTGTC CAACCTCAAG GCTGGAGCCA AGGATATTCT GGTGGCTACA 
GATGTGGCTG GTCGTGGTAT CGATATCCAA GACGTATCTA TGGTTGTCAA CTACGACATG 
GCCAAAAACA TTGAAGATTA CATTCACCGA ATTGGCCGCA CAGGAAGAGC AGGCAAGAGT 
GGTGTAGCCA TCACCTTCCT CACCAAAGAG GACTCAGCCG TGTTCTATGA ACTGAAGCAA 
GCCATCCTGG AGAGCCCAGT GTCCTCTTGC CCTCCAGAGC TGGCAAACCA CCCAGATGCC 
CAGCACAAGC CAGGCACCAT CCTTACCAAG AAGCGCAGGG AAGAGACCAT CTTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257405.1
CDS	141..2600
Translation	MAGELADKKDRDASPKEERKRSRTPERDRDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSTERERRHKERERDKERDRSKKERERDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKGDEDEHGDKRPKIQPLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQKMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA

Target ORF information:

RefSeq Version	XM_006257343.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 23 (Ddx23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257343.3

ORF Insert Sequence:

ATGGCAGGAG AACTGGCTGA CAAGAAGGAC CGAGACGCAT CACCCAAGGA AGAAAGGAAG 
CGATCGCGGA CTCCTGAAAG AGATCGCGAT AGAGACCGGG ACCGGAAGTC CTCCCCATCT 
AAAGACCGGA AGAGGCATCG CTCACGGGAT AGGCGTCGAG GAGGTAGTCG TTCTCGCTCT 
CGGTCCCGTT CCAAGTCTAC AGAGAGGGAA CGACGGCACA AAGAACGGGA ACGAGATAAA 
GAACGGGATC GGAGTAAGAA GGAGCGGGAG CGGGACAAGG ATGGCCACCG GCGGGACAAG 
GACCGCAAGA GATCCAGCTT GTCTCCTGGT CGAGGAAAAG ATTTCAAGTC TCGGAAGGAC 
AGAGATTCTA AGAAGGGTGA CGAGGATGAG CATGGTGACA AGAGGCCTAA GATCCAGCCA 
CTGTCCCTGG AGGAACTTCT GGCCAAGAAA AAGGCTGAGG AAGAAGCTGA AGCTAAGCCT 
AAATTCCTCT CCAAAGCAGA GCGAGAGGCT GAAGCTCTGA AGCGGCGGCA GCAGGAGGTG 
GAGGAGCGAC AGAAGATGCT CGAAGAAGAG AGGAAGAAGA GGAAACAGTT CCAGGATTTG 
GGCAGGAAGA TGCTGGAAGA CCCTCAGGAA AGGGAACGGA GGGAACGCAG GGAAAGGATG 
GAGCGAGAGA CCAATGGCAA CGAGGATGAG GAGGGCCGGC AGAAGATCCG GGAGGAGAAG 
GACAAGAGCA AGGAGCTGCA CGCTATTAAG GAGCGTTACC TGGGTGGCAT CAAAAAACGG 
CGCCGGACAA GGCATCTCAA TGACCGCAAG TTTGTGTTTG AGTGGGATGC GTCTGAAGAC 
ACCTCCATTG ACTACAACCC CCTGTACAAA GAACGGCACC AGGTGCAGCT GTTGGGGCGG 
GGCTTCATTG CGGGCATTGA CCTAAAGCAG CAGAAGCGAG AACAGTCACG TTTCTATGGA 
GACCTAATGG AAAAGAGACG AACCCTGGAA GAAAAGGAGC AGGAGGAAGC AAGACTCCGC 
AAACTTCGTA AGAAGGAAGC CAAGCAACGC TGGGATGATC GCCACTGGTC CCAGAAGAAG 
TTAGATGAGA TGACAGACAG GGACTGGCGG ATCTTTCGGG AAGACTATAG CATCACCACC 
AAAGGTGGCA AGATTCCCAA TCCTATTCGG TCCTGGAAAG ATTCGTCTCT GCCCCCACAT 
ATCTTGGAAG TCATTGATAA GTGTGGCTAC AAGGAGCCAA CACCTATCCA GCGTCAGGCC 
ATTCCCATTG GGCTTCAGAA TCGTGACATC ATTGGCGTGG CTGAGACTGG CAGTGGCAAG 
ACAGCAGCCT TCCTCATCCC ATTACTGGTC TGGATCACCA CACTTCCCAA AATTGACAGG 
ATCGAAGAGT CCGACCAGGG CCCTTATGCC ATCATCCTGG CTCCCACCCG TGAATTGGCT 
CAGCAGATCG AGGAAGAGAC CATCAAGTTC GGAAAGCCAC TAGGTATCCG CACTGTGGCT 
GTCATTGGTG GCATCTCCAG AGAGGACCAA GGCTTCAGGC TACGCATGGG TTGTGAGATT 
GTGATCGCCA CCCCAGGACG TCTGATTGAC GTGCTGGAGA ACCGTTACCT GGTGCTCAGC 
CGCTGCACCT ATGTGGTTCT GGATGAGGCC GACAGGATGA TTGACATGGG CTTCGAGCCA 
GATGTGCAGA AGATCCTGGA GCACATGCCT GTCAGCAACC AGAAGCCGGA CACGGACGAG 
GCCGAGGACC CCGAGAAGAT GTTAGCCAAC TTTGAATCAG GAAAACACAA GTACCGTCAA 
ACAGTCATGT TCACGGCTAC CATGCCTCCA GCGGTGGAGC GTCTAGCCAG GAGCTATCTT 
CGGCGGCCTG CTGTGGTGTA CATTGGCTCT GCAGGCAAGC CCCATGAGCG GGTGGAACAG 
AAGGTCTTTC TCATGTCGGA GTCAGAAAAG AGGAAAAAGC TGCTGGCAAT CCTGGAACAA 
GGTTTTGATC CACCCATCAT CATCTTTGTC AACCAGAAGA AGGGTTGCGA CGTGTTGGCC 
AAGTCTCTGG AGAAGATGGG GTACAACGCC TGTACACTGC ATGGTGGCAA AGGCCAGGAG 
CAGCGAGAAT TCGCATTGTC CAACCTCAAG GCTGGAGCCA AGGATATTCT GGTGGCTACA 
GATGTGGCTG GTCGTGGTAT CGATATCCAA GACGTATCTA TGGTTGTCAA CTACGACATG 
GCCAAAAACA TTGAAGATTA CATTCACCGA ATTGGCCGCA CAGGAAGAGC AGGCAAGAGT 
GGTGTAGCCA TCACCTTCCT CACCAAAGAG GACTCAGCCG TGTTCTATGA ACTGAAGCAA 
GCCATCCTGG AGAGCCCAGT GTCCTCTTGC CCTCCAGAGC TGGCAAACCA CCCAGATGCC 
CAGCACAAGC CAGGCACCAT CCTTACCAAG AAGCGCAGGG AAGAGACCAT CTTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Biochimica et biophysica acta1829(8)764-74(2013 Aug)
Chang TH,Tung L,Yeh FL,Chen JH,Chang SL

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

The spliceosome: design principles of a dynamic RNP machine.
Cell136(4)701-18(2009 Feb)
Wahl MC,Will CL,L?hrmann R

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
RNA (New York, N.Y.)8(4)426-39(2002 Apr)
Jurica MS,Licklider LJ,Gygi SR,Grigorieff N,Moore MJ

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Ddx23 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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