Ext1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ext1
Entrez Gene ID	299907
Full Name	exostosin glycosyltransferase 1
General protein information	Preferred Names exostosin glycosyltransferase 1 Names exostosin-1 exostoses (multiple) 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130540.1	NP_001124012.1	exostosin-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ext1b	NP_001012369.2
Xenopus tropicalis (tropical clawed frog)	ext1	NP_001006730.1
Homo sapiens (human)	EXT1	NP_000118.2
	EXT1	XP_001096808.1
Gallus gallus (chicken)	LOC100858205	XP_003640902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010388	XP_311558.3
Canis lupus familiaris (dog)	EXT1	XP_539145.2
Bos taurus (cattle)	EXT1	NP_001091564.1
Mus musculus (house mouse)	Ext1	NP_034292.2
Rattus norvegicus (Norway rat)	Ext1	NP_001124012.1
Caenorhabditis elegans (roundworm)	rib-1	NP_502180.1
Pan troglodytes (chimpanzee)	EXT1	XP_001141496.1
Drosophila melanogaster (fruit fly)	ttv	NP_477231.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of exon 8 from the EXT1 gene causes multiple osteochondromas (MO) in a family with three affected members.
Zhuang L, Gerber SD, Kuchen S, Villiger PM, Trueb B
SpringerPlus571(2016)

Identification of a novel EXT1 mutation in patients with hereditary multiple exostosis by exome sequencing.
Liu H, Wu S, Duan L, Zhu W, Zhang S, Hu X, Jia W, Yang G, Liu C, Li W, Yang L, Guo L, Lin Y, Wang Y, He M, Yang Z, He Y, Cai Z, Wang D
Oncology reports33(2)547-52(2015 Feb)

Novel EXT1 mutation identified in a pedigree with hereditary multiple exostoses.
Cao L, Liu F, Kong M, Fang Y, Gu H, Chen Y, Zhao C, Zhang S, Bi Q
Oncology reports31(2)713-8(2014 Feb)

Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.
Shevelev OB, Rykova VI, Fedoseeva LA, Leberfarb EY, Dymshits GM, Kolosova NG
Biochemistry. Biokhimiia77(1)56-61(2012 Jan)

Synovial joint formation requires local Ext1 expression and heparan sulfate production in developing mouse embryo limbs and spine.
Mundy C, Yasuda T, Kinumatsu T, Yamaguchi Y, Iwamoto M, Enomoto-Iwamoto M, Koyama E, Pacifici M
Developmental biology351(1)70-81(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ext1 gene is involved in heparan sulfate proteoglycans metabolism.
Title: Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.

The following Ext1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ext1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02425	NM_001130540.1 Latest version!	Rattus norvegicus exostosin glycosyltransferase 1 (Ext1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ext1 ( NM_001130540.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001124012.1 Rattus norvegicus exostosin glycosyltransferase 1 (Ext1), mRNA.

Price & Availability↑

CloneID	ORa02425
Clone ID Related Accession (Same CDS sequence)	NM_001130540.1
Accession Version	NM_001130540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	exostosin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM064038.1, BE126756.1, BC105839.1, FM083888.1 and DV719494.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105839.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGCCA AAAAACGCTA TTTCATCCTG CTCTCAGCTG GCTCTTGTCT CGCCCTTTTG 
TTTTATTTTG GAGGCGTGCA GTTTAGGGCA TCGAGGAGCC ACAGCCGGAG AGAAGAGCAC 
AGTGGTCGGA ATGGCTTGCA CCAACCCAGT CCGGATCATT TCTGGCCCCG CTTCCCGGAC 
GCTCTGCGCC CCTTCTTTCC TTGGGATCAA TTGGAAAACG AGGATTCCAG CGTGCACATT 
TCCCCCCGGC AGAAGCGGGA CGCCAACTCG AGCATCTACA AAGGCAAGAA GTGCCGCATG 
GAGTCCTGCT TCGATTTCAC CCTTTGCAAG AAAAACGGCT TCAAAGTCTA CGTCTACCCG 
CAGCAGAAAG GGGAGAAAAT TGCCGAAAGT TACCAAAACA TTCTAGCGGC CATCGAGGGC 
TCCAGGTTCT ACACCTCGGA CCCCAGCCAG GCGTGCCTCT TTGTCCTGAG TCTGGATACT 
TTAGACAGAG ACCAGTTATC ACCTCAGTAT GTGCACAATT TGAGATCCAA AGTGCAGAGT 
CTCCACTTGT GGAACAATGG TAGGAATCAT TTAATTTTTA ATTTATATTC TGGCACTTGG 
CCTGACTACA CCGAGGACGT GGGGTTTGAC ATCGGCCAGG CGATGCTGGC CAAAGCCAGC 
ATCAGTACTG AAAACTTCCG ACCAAACTTT GATGTTTCTA TTCCCCTCTT TTCTAAGGAT 
CATCCCAGGA CAGGAGGGGA GAGGGGGTTT TTGAAATTTA ACACCATCCC TCCCCTCAGG 
AAGTACATGC TGGTATTCAA GGGGAAGAGG TACCTGACAG GGATAGGGTC AGACACCAGG 
AATGCCTTAT ATCACGTCCA TAACGGGGAG GACGTCTTGC TCCTCACCAC CTGCAAGCAT 
GGCAAAGACT GGCAAAAGCA CAAGGATTCT CGCTGTGACA GAGACAACAC CGAGTATGAG 
AAATATGATT ATCGGGAAAT GCTGCACAAT GCCACTTTCT GTCTGGTTCC TCGTGGTCGC 
AGGCTTGGGT CCTTCAGATT CCTGGAGGCT TTGCAGGCTG CCTGTGTCCC TGTAATGCTC 
AGCAATGGAT GGGAGTTGCC ATTCTCCGAA GTGATAAATT GGAACCAAGC TGCCGTCATA 
GGCGATGAGA GATTGCTATT ACAGATTCCT TCTACAATCA GGTCTATTCA TCAGGATAAA 
ATCCTAGCAC TTAGACAGCA GACACAGTTC TTGTGGGAGG CTTATTTTTC TTCAGTTGAG 
AAGATTGTAT TAACTACACT AGAGATTATT CAGGACAGAA TATTCAAGCA CATATCACGT 
AACAGTTTAA TATGGAACAA ACATCCTGGA GGATTGTTCG TCCTACCACA GTATTCATCC 
TACCTGGGAG ATTTTCCTTA CTACTATGCT AATTTAGGTC TAAAGCCCCC CTCCAAATTC 
ACTGCAGTCA TCCATGCTGT GACCCCCCTG GTCTCTCAGT CCCAGCCAGT GTTGAAGCTT 
CTTGTGGCAG CAGCCAAATC CCAGTACTGT GCACAGATTA TAGTTCTGTG GAATTGTGAC 
AAGCCTCTAC CAGCCAAACA CCGCTGGCCT GCCACTGCCG TGCCTGTCAT CGTCATTGAA 
GGAGAAAGCA AGGTTATGAG CAGCCGGTTT CTGCCCTATG ACAACATCAT CACTGATGCT 
GTGCTCAGCC TTGATGAGGA CACTGTGCTT TCAACCACGG AAGTGGATTT TGCCTTCACT 
GTATGGCAGA GCTTCCCGGA GAGGATTGTG GGATATCCTG CACGCAGCCA TTTCTGGGAT 
AACTCAAAGG AGCGATGGGG ATATACATCC AAGTGGACGA ATGACTACTC CATGGTGTTG 
ACAGGAGCTG CTATCTACCA CAAATATTAT CACTACCTGT ATTCCCATTA CCTGCCAGCC 
AGCCTGAAGA ACATGGTAGA CCAACTGGCC AACTGTGAGG ACATTCTCAT GAATTTCCTG 
GTGTCTGCTG TGACGAAATT GCCTCCAATC AAAGTGACCC AGAAGAAACA GTATAAGGAG 
ACAATGATGG GACAGACTTC CCGAGCATCT CGCTGGGCTG ACCCTGACCA CTTTGCCCAG 
CGACAGAGCT GCATGAATAC GTTTGCCAGC TGGTTTGGCT ACATGCCGCT GATCCACTCT 
CAGATGAGGC TCGACCCGGT CCTCTTTAAA GACCAAGTCT CAATCCTGAG GAAGAAATAC 
AGAGACATTG AACGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124012.1
CDS	121..2361
Translation	MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFPDALRPFFPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL

Target ORF information:

RefSeq Version	NM_001130540.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus exostosin glycosyltransferase 1 (Ext1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130540.1

ORF Insert Sequence:

ATGCAGGCCA AAAAACGCTA TTTCATCCTG CTCTCAGCTG GCTCTTGTCT CGCCCTTTTG 
TTTTATTTTG GAGGCGTGCA GTTTAGGGCA TCGAGGAGCC ACAGCCGGAG AGAAGAGCAC 
AGTGGTCGGA ATGGCTTGCA CCAACCCAGT CCGGATCATT TCTGGCCCCG CTTCCCGGAC 
GCTCTGCGCC CCTTCTTTCC TTGGGATCAA TTGGAAAACG AGGATTCCAG CGTGCACATT 
TCCCCCCGGC AGAAGCGGGA CGCCAACTCG AGCATCTACA AAGGCAAGAA GTGCCGCATG 
GAGTCCTGCT TCGATTTCAC CCTTTGCAAG AAAAACGGCT TCAAAGTCTA CGTCTACCCG 
CAGCAGAAAG GGGAGAAAAT TGCCGAAAGT TACCAAAACA TTCTAGCGGC CATCGAGGGC 
TCCAGGTTCT ACACCTCGGA CCCCAGCCAG GCGTGCCTCT TTGTCCTGAG TCTGGATACT 
TTAGACAGAG ACCAGTTATC ACCTCAGTAT GTGCACAATT TGAGATCCAA AGTGCAGAGT 
CTCCACTTGT GGAACAATGG TAGGAATCAT TTAATTTTTA ATTTATATTC TGGCACTTGG 
CCTGACTACA CCGAGGACGT GGGGTTTGAC ATCGGCCAGG CGATGCTGGC CAAAGCCAGC 
ATCAGTACTG AAAACTTCCG ACCAAACTTT GATGTTTCTA TTCCCCTCTT TTCTAAGGAT 
CATCCCAGGA CAGGAGGGGA GAGGGGGTTT TTGAAATTTA ACACCATCCC TCCCCTCAGG 
AAGTACATGC TGGTATTCAA GGGGAAGAGG TACCTGACAG GGATAGGGTC AGACACCAGG 
AATGCCTTAT ATCACGTCCA TAACGGGGAG GACGTCTTGC TCCTCACCAC CTGCAAGCAT 
GGCAAAGACT GGCAAAAGCA CAAGGATTCT CGCTGTGACA GAGACAACAC CGAGTATGAG 
AAATATGATT ATCGGGAAAT GCTGCACAAT GCCACTTTCT GTCTGGTTCC TCGTGGTCGC 
AGGCTTGGGT CCTTCAGATT CCTGGAGGCT TTGCAGGCTG CCTGTGTCCC TGTAATGCTC 
AGCAATGGAT GGGAGTTGCC ATTCTCCGAA GTGATAAATT GGAACCAAGC TGCCGTCATA 
GGCGATGAGA GATTGCTATT ACAGATTCCT TCTACAATCA GGTCTATTCA TCAGGATAAA 
ATCCTAGCAC TTAGACAGCA GACACAGTTC TTGTGGGAGG CTTATTTTTC TTCAGTTGAG 
AAGATTGTAT TAACTACACT AGAGATTATT CAGGACAGAA TATTCAAGCA CATATCACGT 
AACAGTTTAA TATGGAACAA ACATCCTGGA GGATTGTTCG TCCTACCACA GTATTCATCC 
TACCTGGGAG ATTTTCCTTA CTACTATGCT AATTTAGGTC TAAAGCCCCC CTCCAAATTC 
ACTGCAGTCA TCCATGCTGT GACCCCCCTG GTCTCTCAGT CCCAGCCAGT GTTGAAGCTT 
CTTGTGGCAG CAGCCAAATC CCAGTACTGT GCACAGATTA TAGTTCTGTG GAATTGTGAC 
AAGCCTCTAC CAGCCAAACA CCGCTGGCCT GCCACTGCCG TGCCTGTCAT CGTCATTGAA 
GGAGAAAGCA AGGTTATGAG CAGCCGGTTT CTGCCCTATG ACAACATCAT CACTGATGCT 
GTGCTCAGCC TTGATGAGGA CACTGTGCTT TCAACCACGG AAGTGGATTT TGCCTTCACT 
GTATGGCAGA GCTTCCCGGA GAGGATTGTG GGATATCCTG CACGCAGCCA TTTCTGGGAT 
AACTCAAAGG AGCGATGGGG ATATACATCC AAGTGGACGA ATGACTACTC CATGGTGTTG 
ACAGGAGCTG CTATCTACCA CAAATATTAT CACTACCTGT ATTCCCATTA CCTGCCAGCC 
AGCCTGAAGA ACATGGTAGA CCAACTGGCC AACTGTGAGG ACATTCTCAT GAATTTCCTG 
GTGTCTGCTG TGACGAAATT GCCTCCAATC AAAGTGACCC AGAAGAAACA GTATAAGGAG 
ACAATGATGG GACAGACTTC CCGAGCATCT CGCTGGGCTG ACCCTGACCA CTTTGCCCAG 
CGACAGAGCT GCATGAATAC GTTTGCCAGC TGGTTTGGCT ACATGCCGCT GATCCACTCT 
CAGATGAGGC TCGACCCGGT CCTCTTTAAA GACCAAGTCT CAATCCTGAG GAAGAAATAC 
AGAGACATTG AACGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of exon 8 from the EXT1 gene causes multiple osteochondromas (MO) in a family with three affected members.
SpringerPlus571(2016)
Zhuang L,Gerber SD,Kuchen S,Villiger PM,Trueb B

Identification of a novel EXT1 mutation in patients with hereditary multiple exostosis by exome sequencing.
Oncology reports33(2)547-52(2015 Feb)
Liu H,Wu S,Duan L,Zhu W,Zhang S,Hu X,Jia W,Yang G,Liu C,Li W,Yang L,Guo L,Lin Y,Wang Y,He M,Yang Z,He Y,Cai Z,Wang D

Novel EXT1 mutation identified in a pedigree with hereditary multiple exostoses.
Oncology reports31(2)713-8(2014 Feb)
Cao L,Liu F,Kong M,Fang Y,Gu H,Chen Y,Zhao C,Zhang S,Bi Q

Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.
Biochemistry. Biokhimiia77(1)56-61(2012 Jan)
Shevelev OB,Rykova VI,Fedoseeva LA,Leberfarb EY,Dymshits GM,Kolosova NG

Synovial joint formation requires local Ext1 expression and heparan sulfate production in developing mouse embryo limbs and spine.
Developmental biology351(1)70-81(2011 Mar)
Mundy C,Yasuda T,Kinumatsu T,Yamaguchi Y,Iwamoto M,Enomoto-Iwamoto M,Koyama E,Pacifici M

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Ext1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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