Gadd45b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gadd45b
Entrez Gene ID	299626
Full Name	growth arrest and DNA-damage-inducible, beta
General protein information	Preferred Names growth arrest and DNA-damage-inducible, beta Names growth arrest and DNA damage-inducible protein GADD45 beta growth arrest and DNA-damage-inducible 45 beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240888.3	XP_006240950.1	growth arrest and DNA damage-inducible protein GADD45 beta isoform X1
NM_001008321.1	NP_001008322.1	growth arrest and DNA damage-inducible protein GADD45 beta

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GADD45B	XP_001151983.1
Canis lupus familiaris (dog)	GADD45B	XP_542187.1
Gallus gallus (chicken)	LOC100859853	XP_003643868.2
Xenopus tropicalis (tropical clawed frog)	gadd45b	XP_002941163.2
	GADD45B	XP_001098598.1
Bos taurus (cattle)	GADD45B	NP_001035694.1
Mus musculus (house mouse)	Gadd45b	NP_032681.1
Danio rerio (zebrafish)	gadd45ba	NP_998196.1
Danio rerio (zebrafish)	gadd45bb	NP_001012386.1
Rattus norvegicus (Norway rat)	Gadd45b	NP_001008322.1
Homo sapiens (human)	GADD45B	NP_056490.2

Gene Ontology
Bibliography

Related articles in PubMed

Sex-Specific Effects of Prenatal Stress on Bdnf Expression in Response to an Acute Challenge in Rats: a Role for Gadd45?.
Luoni A, Berry A, Raggi C, Bellisario V, Cirulli F, Riva MA
Molecular neurobiology53(10)7037-7047(2016 Dec)

Nonylphenol induces liver toxicity and oxidative stress in rat.
Kazemi S, Mousavi Kani SN, Ghasemi-Kasman M, Aghapour F, Khorasani H, Moghadamnia AA
Biochemical and biophysical research communications479(1)17-21(2016 10)

Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.
Kigar SL, Chang L, Hayne MR, Karls NT, Auger AP
Brain research1642461-466(2016 07)

Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury.
He G, Xu W, Tong L, Li S, Su S, Tan X, Li C
Apoptosis : an international journal on programmed cell death21(4)390-403(2016 Apr)

Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells.
He GQ, Xu WM, Li JF, Li SS, Liu B, Tan XD, Li CQ
Molecular brain888(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

exposure to an acute stress upregulates Bdnf expression in the prefrontal cortex which is selectively impaired in prenatal stress female rats, an effect that was associated with changes in the modulation of the DNA demethylase Gadd45beta.
Title: Sex-Specific Effects of Prenatal Stress on Bdnf Expression in Response to an Acute Challenge in Rats: a Role for Gadd45?.

Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress.
Title: Nonylphenol induces liver toxicity and oxidative stress in rat.

Report of sex differences in the expression of growth arrest and DNA damage inducible factor beta (Gadd45b), but not family members alpha (a) or gamma (g), in the neonatal and juvenile rodent amygdala
Title: Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.

The data suggested that Gadd45b inhibited autophagy and apoptosis under oxygen-glucose deprivation/reperfusion injury. Gadd45b may be a common regulatory protein to control autophagy and apoptosis.
Title: Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury.

Gadd45b knockdown decreased BDNF expression and the p-CREB pathway after brain neuron oxygen-glucose deprivation and reperfusion. It increased methylation of the 4th CpG island (105 bp apart from BDNF IXa).
Title: Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells.

The following Gadd45b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gadd45b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11413	NM_001008321.1 Latest version!	Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa29835	XM_006240888.3 Latest version!	Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gadd45b ( NM_001008321.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008322.1 Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), mRNA.

Price & Availability↑

CloneID	ORa11413
Clone ID Related Accession (Same CDS sequence)	NM_001008321.1
Accession Version	NM_001008321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	growth arrest and DNA damage-inducible protein GADD45 beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085337.1. On Dec 16, 2004 this sequence version replaced XM_216846.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085337.1, DN947934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTGG AAGAGCTGGT GGCGAGCGAC AACGCGGTTC AGAAGATGCA GGCGGTGACT 
GCGGCGGTGG AGCAGCTGCT GGTGGCCGCG CAGCGTCAGG ATCGCCTCAC CGTGGGGGTG 
TACGAGGCGG CCAAACTGAT GAATGTGGAC CCCGACAGCG TGGTCTTGTG CCTGCTGGCC 
ATAGACGAAG AAGAGGAGGA TGATATTGCT CTGCAAATTC ACTTCACCCT GATCCAATCG 
TTCTGCTGCG ACAATGACAT TGACATCGTC AGGGTATCAG GCATGCAAAG GCTGGCGCAG 
CTCCTGGGTG AGCCGGCAGA GACTCTAGGC ACAAACGAGG CCCGAGACCT GCACTGCCTC 
CTGGTCACGA ACTGTCATAC AGATTCCTGG AAAAGCCAAG GCTTGGTGGA GGTGGCCAGT 
TACTGTGAAG AGAGCAGAGG CAATAACCAG TGGGTCCCCT ATATCTCTCT GGAGGAACGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008322.1
CDS	233..715
Translation	MTLEELVASDNAVQKMQAVTAAVEQLLVAAQRQDRLTVGVYEAAKLMNVDPDSVVLCLLAIDEEEEDDIALQIHFTLIQSFCCDNDIDIVRVSGMQRLAQLLGEPAETLGTNEARDLHCLLVTNCHTDSWKSQGLVEVASYCEESRGNNQWVPYISLEER

Target ORF information:

RefSeq Version	NM_001008321.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008321.1

ORF Insert Sequence:

ATGACCCTGG AAGAGCTGGT GGCGAGCGAC AACGCGGTTC AGAAGATGCA GGCGGTGACT 
GCGGCGGTGG AGCAGCTGCT GGTGGCCGCG CAGCGTCAGG ATCGCCTCAC CGTGGGGGTG 
TACGAGGCGG CCAAACTGAT GAATGTGGAC CCCGACAGCG TGGTCTTGTG CCTGCTGGCC 
ATAGACGAAG AAGAGGAGGA TGATATTGCT CTGCAAATTC ACTTCACCCT GATCCAATCG 
TTCTGCTGCG ACAATGACAT TGACATCGTC AGGGTATCAG GCATGCAAAG GCTGGCGCAG 
CTCCTGGGTG AGCCGGCAGA GACTCTAGGC ACAAACGAGG CCCGAGACCT GCACTGCCTC 
CTGGTCACGA ACTGTCATAC AGATTCCTGG AAAAGCCAAG GCTTGGTGGA GGTGGCCAGT 
TACTGTGAAG AGAGCAGAGG CAATAACCAG TGGGTCCCCT ATATCTCTCT GGAGGAACGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gadd45b ( XM_006240888.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240950.1 Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29835
Clone ID Related Accession (Same CDS sequence)	XM_006240888.3
Accession Version	XM_006240888.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	growth arrest and DNA damage-inducible protein GADD45 beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGG AAGAGCTGGT GGCGAGCGAC AACGCGGTTC AGAAGATGCA GGCGGTGACT 
GCGGCGGTGG AGCAGCTGCT GGTGGCCGCG CAGCGTCAGG ATCGCCTCAC CGTGGGGGTG 
TACGAGGCGG CCAAACTGAT GAATGTGGAC CCCGACAGCG TGGTCTTGTG CCTGCTGGCC 
ATAGACGAAG AAGAGGAGGA TGATATTGCT CTGCAAATTC ACTTCACCCT GATCCAATCG 
TTCTGCTGCG ACAATGACAT TGACATCGTC AGGGTATCAG GCATGCAAAG GCTGGCGCAG 
CTCCTGGGTG AGCCGGCAGA GACTCTAGGC ACAAACGAGG CCCGAGACCT GCACTGCCTC 
CTGGTCACGG TGAGTGACCC ACCCCGCCCT GATCACCTGG GTCCCGTGTT TGTCAACATA 
GCTGGGCTGG CTGCTCCAAC ACCAGCTCAC TGCCCAGCCA TGCTCGGCAT GTCCCGTGGG 
CTGCCTCGTC ACCCTGGCAG CTATTTTGAG CTAGCCTGTT TTTCTCTAAT AGGGCTTCTG 
GAGCTGAACA GAGGCTCCCT ACCGAACTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240950.1
CDS	259..831
Translation	MTLEELVASDNAVQKMQAVTAAVEQLLVAAQRQDRLTVGVYEAAKLMNVDPDSVVLCLLAIDEEEEDDIALQIHFTLIQSFCCDNDIDIVRVSGMQRLAQLLGEPAETLGTNEARDLHCLLVTVSDPPRPDHLGPVFVNIAGLAAPTPAHCPAMLGMSRGLPRHPGSYFELACFSLIGLLELNRGSLPNS

Target ORF information:

RefSeq Version	XM_006240888.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth arrest and DNA-damage-inducible, beta (Gadd45b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240888.3

ORF Insert Sequence:

ATGACCCTGG AAGAGCTGGT GGCGAGCGAC AACGCGGTTC AGAAGATGCA GGCGGTGACT 
GCGGCGGTGG AGCAGCTGCT GGTGGCCGCG CAGCGTCAGG ATCGCCTCAC CGTGGGGGTG 
TACGAGGCGG CCAAACTGAT GAATGTGGAC CCCGACAGCG TGGTCTTGTG CCTGCTGGCC 
ATAGACGAAG AAGAGGAGGA TGATATTGCT CTGCAAATTC ACTTCACCCT GATCCAATCG 
TTCTGCTGCG ACAATGACAT TGACATCGTC AGGGTATCAG GCATGCAAAG GCTGGCGCAG 
CTCCTGGGTG AGCCGGCAGA GACTCTAGGC ACAAACGAGG CCCGAGACCT GCACTGCCTC 
CTGGTCACGG TGAGTGACCC ACCCCGCCCT GATCACCTGG GTCCCGTGTT TGTCAACATA 
GCTGGGCTGG CTGCTCCAAC ACCAGCTCAC TGCCCAGCCA TGCTCGGCAT GTCCCGTGGG 
CTGCCTCGTC ACCCTGGCAG CTATTTTGAG CTAGCCTGTT TTTCTCTAAT AGGGCTTCTG 
GAGCTGAACA GAGGCTCCCT ACCGAACTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sex-Specific Effects of Prenatal Stress on Bdnf Expression in Response to an Acute Challenge in Rats: a Role for Gadd45?.
Molecular neurobiology53(10)7037-7047(2016 Dec)
Luoni A,Berry A,Raggi C,Bellisario V,Cirulli F,Riva MA

Nonylphenol induces liver toxicity and oxidative stress in rat.
Biochemical and biophysical research communications479(1)17-21(2016 10)
Kazemi S,Mousavi Kani SN,Ghasemi-Kasman M,Aghapour F,Khorasani H,Moghadamnia AA

Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.
Brain research1642461-466(2016 07)
Kigar SL,Chang L,Hayne MR,Karls NT,Auger AP

Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury.
Apoptosis : an international journal on programmed cell death21(4)390-403(2016 Apr)
He G,Xu W,Tong L,Li S,Su S,Tan X,Li C

Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells.
Molecular brain888(2015 Dec)
He GQ,Xu WM,Li JF,Li SS,Liu B,Tan XD,Li CQ

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Gadd45b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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