Cpsf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpsf3
Entrez Gene ID	298916
Full Name	cleavage and polyadenylation specific factor 3
General protein information	Preferred Names cleavage and polyadenylation specific factor 3 Names cleavage and polyadenylation specificity factor subunit 3 cleavage and polyadenylation specific factor 3, 73kDa cleavage and polyadenylation specificity factor 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030030.1	NP_001025201.1	cleavage and polyadenylation specificity factor subunit 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPSF3	NP_057291.1
Caenorhabditis elegans (roundworm)	cpsf-3	NP_502553.2
Oryza sativa (rice)	Os03g0852900	NP_001051928.1
Canis lupus familiaris (dog)	CPSF3	XP_005630164.1
Mus musculus (house mouse)	Cpsf3	NP_061283.2
Danio rerio (zebrafish)	cpsf3	NP_001003836.1
Kluyveromyces lactis	KLLA0C04598g	XP_452404.1
Eremothecium gossypii	AGOS_AGR279C	NP_986945.2
Magnaporthe oryzae (rice blast fungus)	MGG_01804	XP_003714815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001224	XP_321933.5
Saccharomyces cerevisiae (baker's yeast)	YSH1	NP_013379.1
Schizosaccharomyces pombe (fission yeast)	ysh1	NP_594263.2
Arabidopsis thaliana (thale cress)	CPSF73-I	NP_001031215.1
Pan troglodytes (chimpanzee)	CPSF3	NP_001233296.1
	CPSF3	XP_002799184.1
Bos taurus (cattle)	CPSF3	NP_776709.1
Rattus norvegicus (Norway rat)	Cpsf3	NP_001025201.1
Gallus gallus (chicken)	CPSF3	XP_419942.4
Drosophila melanogaster (fruit fly)	Cpsf73	NP_650738.1
Neurospora crassa	NCU03479	XP_956368.1
Xenopus tropicalis (tropical clawed frog)	cpsf3	NP_001006770.1

Gene Ontology
Bibliography

Related articles in PubMed

Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors.
Gruber AR, Martin G, Keller W, Zavolan M
Wiley interdisciplinary reviews. RNA5(2)183-96(2014 Mar-Apr)

Pre-mRNA 3'-end processing complex assembly and function.
Chan S, Choi EA, Shi Y
Wiley interdisciplinary reviews. RNA2(3)321-35(2011 May-Jun)

The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA.
Laishram RS, Anderson RA
The EMBO journal29(24)4132-45(2010 Dec)

Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing.
Yang XC, Sullivan KD, Marzluff WF, Dominski Z
Molecular and cellular biology29(1)31-42(2009 Jan)

Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation.
Kolev NG, Yario TA, Benson E, Steitz JA
EMBO reports9(10)1013-8(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cpsf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpsf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08051	NM_001030030.1 Latest version!	Rattus norvegicus cleavage and polyadenylation specific factor 3 (Cpsf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpsf3 ( NM_001030030.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001025201.1 Rattus norvegicus cleavage and polyadenylation specific factor 3 (Cpsf3), mRNA.

Price & Availability↑

CloneID	ORa08051
Clone ID Related Accession (Same CDS sequence)	NM_001030030.1
Accession Version	NM_001030030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	cleavage and polyadenylation specificity factor subunit 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099817.1. On Aug 5, 2005 this sequence version replaced XM_216667.3. ##Evidence-Data-START## Transcript exon combination :: BC099817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCAA TTCCTGCAGA GGAGAGCGAC CAGCTGCTGA TCCGACCCCT TGGTGCTGGC 
CAAGAAGTGG GAAGATCATG TATTATTCTG GAGTTCAAAG GAAGGAAAAT CATGCTAGAC 
TGTGGGATCC ACCCTGGCCT GGAAGGAATG GATGCTCTTC CTTACATTGA CCTCATCGAC 
CCAGCTGAGA TCGACCTTCT GTTGATCAGT CACTTCCATT TGGACCACTG TGGAGCCCTG 
CCCTGGTTCC TTCAGAAGAC AAGTTTCAAA GGACGGACGT TTATGACCCA CGCCACAAAA 
GCTATCTATA GATGGCTCCT CTCAGACTAT GTCAAAGTCA GCAACATATC AGCAGATGAC 
ATGCTGTACA CTGAGACAGA CTTGGAAGAA AGCATGGACA AAATCGAAAC CATCAATTTC 
CATGAAGTTA AGGAAGTTGC AGGAATCAAG TTCTGGTGTT ACCATGCCGG CCACGTCCTG 
GGAGCAGCCA TGTTTATGAT AGAGATTGCT GGTATGAAGC TTTTGTATAC AGGTGATTTC 
TCAAGACAAG AGGACAGACA TCTAATGGCC GCTGAAATTC CTAATATTAA GCCAGACATC 
CTGATCATTG AATCTACGTA TGGGACCCAT ATCCATGAGA AGCGTGAAGA GCGAGAAGCA 
AGATTCTGCA ACACTGTGCA CGACATTGTC AACAGAGGGG GCAGAGGGCT CATTCCTGTC 
TTTGCTCTCG GAAGAGCTCA GGAGCTGCTC CTGATTCTAG ATGAGTACTG GCAGAACCAC 
CCAGAGCTGC ACGACATCCC CATTTACTAC GCATCGTCTC TGGCCAAGAA GTGCATGGCC 
GTGTACCAGA CCTACGTGAA CGCCATGAAC GACAAGATCC GGAAGCAGAT CAACATCAAC 
AACCCCTTCG TTTTCAAGCA CATCAGCAAC CTCAAGAGCA TGGATCACTT TGACGACATT 
GGCCCCAGTG TTGTAATGGC CTCACCAGGC ATGATTCAAA ATGGCTTATC CAGAGAGCTG 
TTTGAAAGCT GGTGTACAGA TAAGAGGAAT GGCGTCATCA TCGCAGGGTA CTGTGTAGAA 
GGGACACTTG CCAAGCACAT CATGTCTGAA CCTGAAGAAA TCACTACCAT GTCTGGACAG 
AAGTTGCCAC TGAAAATGTC GGTTGATTAC ATTTCTTTCT CTGCTCACAC AGATTACCAG 
CAAACCAGTG AATTTATTCG TGCTCTGAAG CCGCCTCACG TGATTTTAGT CCATGGGGAA 
CAGAATGAAA TGGCCAGACT GAAAGCAGCA CTGATCCGAG AATATGAAGA TAACGATGAA 
GTTCACATCG AGGTTCACAA TCCTCGGAAC ACAGAGGCCG TGACCCTGAA CTTCAGAGGG 
GAGAAGCTCG CCAAGGTCAT GGGCTTTTTA GCAGACAAGA AGCCAGAACA GGGCCAGCGG 
GTCTCTGGAA TTCTTGTTAA AAGAAACTTT AATTACCACA TACTCTCTCC CTGTGACCTC 
TCGAATTACA CTGACCTGGC CATGAGCACT GTGAAACAGA CCCAAGCCAT CCCATATACT 
GGGCCCTTTT ACCTGCTCTA TTACCAGCTA CAGAAATTGA CGGGTGATGT AGAAGAATTA 
GAAATTCAAG AAAAGCCTGC TTTGAAGGTA TTCAAAAGCA TCACCGTGGT GCAGGAGCCG 
GGCATGGTGG TCCTGGAGTG GCTGGCGAAC CCCTCTAACG ACATGTATGC AGACACAGTG 
ACCACCGTGA TATTGGAAGT TCAGTCGAAT CCAAAAATAA GGAAAGGTGC AGTGCAGAAG 
GTCTCTAAGA AGCTAGAGAT GCACGTTTAC AGCAAGAGGC TTGAGGTCAT GCTCCAGGAC 
ATATTTGGAG AAGACTGTGT CAGTGTGAAA GATGACTCTG TCCTCAGTGT CACGGTGGAT 
GGAAAAACAG CAAATATTAA TTTGGAGACT CGGGCTGTAG AATGTGAAGA AGGAAGTGAG 
GATGATGAGT CCCTCCGAGA AATGGTGGAA CTGGCCGCCC AGAGACTGTA CGAGGCTCTG 
ACGCCGCCAG TGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025201.1
CDS	95..2152
Translation	MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGMKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMIQNGLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFYLLYYQLQKLTGDVEELEIQEKPALKVFKSITVVQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEVMLQDIFGEDCVSVKDDSVLSVTVDGKTANINLETRAVECEEGSEDDESLREMVELAAQRLYEALTPPVH

Target ORF information:

RefSeq Version	NM_001030030.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cleavage and polyadenylation specific factor 3 (Cpsf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030030.1

ORF Insert Sequence:

ATGTCTGCAA TTCCTGCAGA GGAGAGCGAC CAGCTGCTGA TCCGACCCCT TGGTGCTGGC 
CAAGAAGTGG GAAGATCATG TATTATTCTG GAGTTCAAAG GAAGGAAAAT CATGCTAGAC 
TGTGGGATCC ACCCTGGCCT GGAAGGAATG GATGCTCTTC CTTACATTGA CCTCATCGAC 
CCAGCTGAGA TCGACCTTCT GTTGATCAGT CACTTCCATT TGGACCACTG TGGAGCCCTG 
CCCTGGTTCC TTCAGAAGAC AAGTTTCAAA GGACGGACGT TTATGACCCA CGCCACAAAA 
GCTATCTATA GATGGCTCCT CTCAGACTAT GTCAAAGTCA GCAACATATC AGCAGATGAC 
ATGCTGTACA CTGAGACAGA CTTGGAAGAA AGCATGGACA AAATCGAAAC CATCAATTTC 
CATGAAGTTA AGGAAGTTGC AGGAATCAAG TTCTGGTGTT ACCATGCCGG CCACGTCCTG 
GGAGCAGCCA TGTTTATGAT AGAGATTGCT GGTATGAAGC TTTTGTATAC AGGTGATTTC 
TCAAGACAAG AGGACAGACA TCTAATGGCC GCTGAAATTC CTAATATTAA GCCAGACATC 
CTGATCATTG AATCTACGTA TGGGACCCAT ATCCATGAGA AGCGTGAAGA GCGAGAAGCA 
AGATTCTGCA ACACTGTGCA CGACATTGTC AACAGAGGGG GCAGAGGGCT CATTCCTGTC 
TTTGCTCTCG GAAGAGCTCA GGAGCTGCTC CTGATTCTAG ATGAGTACTG GCAGAACCAC 
CCAGAGCTGC ACGACATCCC CATTTACTAC GCATCGTCTC TGGCCAAGAA GTGCATGGCC 
GTGTACCAGA CCTACGTGAA CGCCATGAAC GACAAGATCC GGAAGCAGAT CAACATCAAC 
AACCCCTTCG TTTTCAAGCA CATCAGCAAC CTCAAGAGCA TGGATCACTT TGACGACATT 
GGCCCCAGTG TTGTAATGGC CTCACCAGGC ATGATTCAAA ATGGCTTATC CAGAGAGCTG 
TTTGAAAGCT GGTGTACAGA TAAGAGGAAT GGCGTCATCA TCGCAGGGTA CTGTGTAGAA 
GGGACACTTG CCAAGCACAT CATGTCTGAA CCTGAAGAAA TCACTACCAT GTCTGGACAG 
AAGTTGCCAC TGAAAATGTC GGTTGATTAC ATTTCTTTCT CTGCTCACAC AGATTACCAG 
CAAACCAGTG AATTTATTCG TGCTCTGAAG CCGCCTCACG TGATTTTAGT CCATGGGGAA 
CAGAATGAAA TGGCCAGACT GAAAGCAGCA CTGATCCGAG AATATGAAGA TAACGATGAA 
GTTCACATCG AGGTTCACAA TCCTCGGAAC ACAGAGGCCG TGACCCTGAA CTTCAGAGGG 
GAGAAGCTCG CCAAGGTCAT GGGCTTTTTA GCAGACAAGA AGCCAGAACA GGGCCAGCGG 
GTCTCTGGAA TTCTTGTTAA AAGAAACTTT AATTACCACA TACTCTCTCC CTGTGACCTC 
TCGAATTACA CTGACCTGGC CATGAGCACT GTGAAACAGA CCCAAGCCAT CCCATATACT 
GGGCCCTTTT ACCTGCTCTA TTACCAGCTA CAGAAATTGA CGGGTGATGT AGAAGAATTA 
GAAATTCAAG AAAAGCCTGC TTTGAAGGTA TTCAAAAGCA TCACCGTGGT GCAGGAGCCG 
GGCATGGTGG TCCTGGAGTG GCTGGCGAAC CCCTCTAACG ACATGTATGC AGACACAGTG 
ACCACCGTGA TATTGGAAGT TCAGTCGAAT CCAAAAATAA GGAAAGGTGC AGTGCAGAAG 
GTCTCTAAGA AGCTAGAGAT GCACGTTTAC AGCAAGAGGC TTGAGGTCAT GCTCCAGGAC 
ATATTTGGAG AAGACTGTGT CAGTGTGAAA GATGACTCTG TCCTCAGTGT CACGGTGGAT 
GGAAAAACAG CAAATATTAA TTTGGAGACT CGGGCTGTAG AATGTGAAGA AGGAAGTGAG 
GATGATGAGT CCCTCCGAGA AATGGTGGAA CTGGCCGCCC AGAGACTGTA CGAGGCTCTG 
ACGCCGCCAG TGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors.
Wiley interdisciplinary reviews. RNA5(2)183-96(2014 Mar-Apr)
Gruber AR,Martin G,Keller W,Zavolan M

Pre-mRNA 3'-end processing complex assembly and function.
Wiley interdisciplinary reviews. RNA2(3)321-35(2011 May-Jun)
Chan S,Choi EA,Shi Y

The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA.
The EMBO journal29(24)4132-45(2010 Dec)
Laishram RS,Anderson RA

Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing.
Molecular and cellular biology29(1)31-42(2009 Jan)
Yang XC,Sullivan KD,Marzluff WF,Dominski Z

Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation.
EMBO reports9(10)1013-8(2008 Oct)
Kolev NG,Yario TA,Benson E,Steitz JA

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Cpsf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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