Nlrc4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrc4
Entrez Gene ID	298784
Full Name	NLR family, CARD domain containing 4
Synonyms	Card12,Ipaf
General protein information	Preferred Names NLR family, CARD domain containing 4 Names NLR family CARD domain-containing protein 4 caspase recruitment domain family, member 12 ice protease-activating factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001309432.1	NP_001296361.1	NLR family CARD domain-containing protein 4
XM_008764492.1	XP_008762714.1	NLR family CARD domain-containing protein 4 isoform X2
XM_008764493.2	XP_008762715.1	NLR family CARD domain-containing protein 4 isoform X3
XM_008764491.2	XP_008762713.1	NLR family CARD domain-containing protein 4 isoform X1

Pathways from BioSystems

Homologies

	NLRC4	XP_001105818.2
Bos taurus (cattle)	NLRC4	NP_001179252.2
Homo sapiens (human)	NLRC4	NP_001186067.1
Pan troglodytes (chimpanzee)	NLRC4	XP_515390.2
Xenopus tropicalis (tropical clawed frog)	nlrc4	XP_004914732.1
Mus musculus (house mouse)	Nlrc4	NP_001028539.1
Rattus norvegicus (Norway rat)	Nlrc4	XP_003750157.2

Gene Ontology
Bibliography

Related articles in PubMed

Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation.
Diebolder CA, Halff EF, Koster AJ, Huizinga EG, Koning RI
Structure (London, England : 1993)23(12)2349-57(2015 Dec)

Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization.
Zhang L, Chen S, Ruan J, Wu J, Tong AB, Yin Q, Li Y, David L, Lu A, Wang WL, Marks C, Ouyang Q, Zhang X, Mao Y, Wu H
Science (New York, N.Y.)350(6259)404-9(2015 Oct)

Structural and biochemical basis for induced self-propagation of NLRC4.
Hu Z, Zhou Q, Zhang C, Fan S, Cheng W, Zhao Y, Shao F, Wang HW, Sui SF, Chai J
Science (New York, N.Y.)350(6259)399-404(2015 Oct)

Phosphorylation of NLRC4 is critical for inflammasome activation.
Qu Y, Misaghi S, Izrael-Tomasevic A, Newton K, Gilmour LL, Lamkanfi M, Louie S, Kayagaki N, Liu J, K?m?ves L, Cupp JE, Arnott D, Monack D, Dixit VM
Nature490(7421)539-42(2012 Oct)

NLRC4-driven production of IL-1? discriminates between pathogenic and commensal bacteria and promotes host intestinal defense.
Franchi L, Kamada N, Nakamura Y, Burberry A, Kuffa P, Suzuki S, Shaw MH, Kim YG, N??ez G
Nature immunology13(5)449-56(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nlrc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa17098	XM_008764492.1 Latest version!	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa45572	XM_008764493.2 Latest version!	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa17097	NM_001309432.1 Latest version!	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa17097	XM_008764491.2 Latest version!	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrc4 ( XM_008764492.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762714.1 Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa17098
Clone ID Related Accession (Same CDS sequence)	XM_008764492.1
Accession Version	XM_008764492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NLR family CARD domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ACTATCCGAG GACTGCAAGA TTCTTATGCT 
GTCCGGTTGC AGCAAGGGGC TATGCAAGAA TTCTCAGATG AGCGCAACAT TCAGAGATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762714.1
CDS	233..2935
Translation	MNFIKENSQALIQRMGIVVIKQICDDLFALNVLNGEEVAIICSHRVEQDAARDIVHMILKKGSAACNLFLKSLENWNYPVYQDLTGHSLFHQNLEEDLDVLAQSLKDLYNSPVFKNFFPLGEDIDIIFNLQITFTEPVLWRKDHRHHRVEQMTLGSLLEALKSPCLIEGESGKGKSTLLQKIAMLWASGMCPALNQFKLVFFIRLSSARGGLFETLYDQLVNIPDSISKPTFRALLLKLHKKVLFLLDAYNEFHPQNCPEIEALVKENHRFKNMVIVTTTTECLRHIRHVGALTVEVGDMTEDSARVLIREVLINELAEGLLFQMQESRCLRNLMRTPLFVVITCAIQMGSEEFQAHTQTMLFQTFYDLLIQKNRRRHSGGTSGDFVRSLDYCGDLALEGVFSHKFDFELEDVCSMNEDVLVRTGLLCKYTAQRLRPTYKFFHKSFQEYTAGRRLSSLLKSREPEEVSKGNSYLKKMVSISDITSLYGNLLLYTCGSSTEATRAIMRHLAMVCEHGSLQGLSVTKRPLWRQESIQNLRNTTEQDVLKAINVNSFVECGINLFSESISKSELSQEFEAFFQGKSLYINSENIPDYLFDFFKYLPNCVSALDFVKLDFYGRATVSQDKTGENSSGVHTEGPSTYIPSRAVSLFFNWMQKFKTLEVTLRDISKLNKQDIKYLGKIFSSASNLKLYIKRCAAVAGRLSSVLRTCKNIHSLMVEASPLTTEDEQYITSVTDLQNLSIHDLHTQRLPGGLADSLGNLKNLLKLILDDIRLNEEDAKSLAEGLRNLKKMRLLHLTRLSDMGEGMDYIVKSLSEEPCDLQEMKLVDCCLTANSLKILAQNLHNLVKLSVLDMSENYLEKAGSEALQGLTIRGLQDSYAVRLQQGAMQEFSDERNIQRS

Target ORF information:

RefSeq Version	XM_008764492.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764492.1

ORF Insert Sequence:

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ACTATCCGAG GACTGCAAGA TTCTTATGCT 
GTCCGGTTGC AGCAAGGGGC TATGCAAGAA TTCTCAGATG AGCGCAACAT TCAGAGATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_008764493.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762715.1 Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa45572
Clone ID Related Accession (Same CDS sequence)	XM_008764493.2
Accession Version	XM_008764493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NLR family CARD domain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGAA 
TCTTCACAAT TTGGTCAAGC TGAGCGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762715.1
CDS	233..2785
Translation	MNFIKENSQALIQRMGIVVIKQICDDLFALNVLNGEEVAIICSHRVEQDAARDIVHMILKKGSAACNLFLKSLENWNYPVYQDLTGHSLFHQNLEEDLDVLAQSLKDLYNSPVFKNFFPLGEDIDIIFNLQITFTEPVLWRKDHRHHRVEQMTLGSLLEALKSPCLIEGESGKGKSTLLQKIAMLWASGMCPALNQFKLVFFIRLSSARGGLFETLYDQLVNIPDSISKPTFRALLLKLHKKVLFLLDAYNEFHPQNCPEIEALVKENHRFKNMVIVTTTTECLRHIRHVGALTVEVGDMTEDSARVLIREVLINELAEGLLFQMQESRCLRNLMRTPLFVVITCAIQMGSEEFQAHTQTMLFQTFYDLLIQKNRRRHSGGTSGDFVRSLDYCGDLALEGVFSHKFDFELEDVCSMNEDVLVRTGLLCKYTAQRLRPTYKFFHKSFQEYTAGRRLSSLLKSREPEEVSKGNSYLKKMVSISDITSLYGNLLLYTCGSSTEATRAIMRHLAMVCEHGSLQGLSVTKRPLWRQESIQNLRNTTEQDVLKAINVNSFVECGINLFSESISKSELSQEFEAFFQGKSLYINSENIPDYLFDFFKYLPNCVSALDFVKLDFYGRATVSQDKTGENSSGVHTEGPSTYIPSRAVSLFFNWMQKFKTLEVTLRDISKLNKQDIKYLGKIFSSASNLKLYIKRCAAVAGRLSSVLRTCKNIHSLMVEASPLTTEDEQYITSVTDLQNLSIHDLHTQRLPGGLADSLGNLKNLLKLILDDIRLNEEDAKSLAEGLRNLKKMRLLHLTRLSDMGEGMDYIVKSLSEEPCDLQEMKLVDCCLTANSLKILESSQFGQAERS

Target ORF information:

RefSeq Version	XM_008764493.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764493.2

ORF Insert Sequence:

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGAA 
TCTTCACAAT TTGGTCAAGC TGAGCGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( NM_001309432.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296361.1 Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), mRNA.

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CloneID	ORa17097
Clone ID Related Accession (Same CDS sequence)	NM_001309432.1 , XM_008764491.2
Accession Version	NM_001309432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	NLR family CARD domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07073207.1 and AC139392.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ATCGGCAGGC TTGGCGTGCT AGAACAGCTA 
AGCGCGTTGA TGCTGCCTTG GTGCTGGGAT GCGTACATCA GTCTGCCCAA TCTGCTGAAG 
CAACTGGAGG GGACCCCAGG GCTTGTCAAA CTTGGATTGA AAAACTGGAG GCTCAGAGAC 
GAAGAGATTA GAAGTTTCGG TGAATTTTTG GAGATGAATC CTCTGAGAGA CTTGCAGCAG 
TTGGATTTAG CAGGACACGG TGTGAGCAGT GATGGATGGC TTTCTTTCAT GGATGTGTTT 
GAGAATCTTA AGCAGTTGGT ATTTTTTGAC TTCGGCACTG AAGAGTTCTT ACCAGACGCA 
GCGCTGGTGA GGAAACTCGG TCAAGTGTTA TCTAAGCTAA CTCTGCTGCA AGAGGCAAGG 
CTCACAGGCT GGGAGCTTGA TGACTATGAC ATAAGTGTTA TTAAAGGAAC CTTTAAACTA 
GTGACTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296361.1
CDS	105..3176
Translation	MNFIKENSQALIQRMGIVVIKQICDDLFALNVLNGEEVAIICSHRVEQDAARDIVHMILKKGSAACNLFLKSLENWNYPVYQDLTGHSLFHQNLEEDLDVLAQSLKDLYNSPVFKNFFPLGEDIDIIFNLQITFTEPVLWRKDHRHHRVEQMTLGSLLEALKSPCLIEGESGKGKSTLLQKIAMLWASGMCPALNQFKLVFFIRLSSARGGLFETLYDQLVNIPDSISKPTFRALLLKLHKKVLFLLDAYNEFHPQNCPEIEALVKENHRFKNMVIVTTTTECLRHIRHVGALTVEVGDMTEDSARVLIREVLINELAEGLLFQMQESRCLRNLMRTPLFVVITCAIQMGSEEFQAHTQTMLFQTFYDLLIQKNRRRHSGGTSGDFVRSLDYCGDLALEGVFSHKFDFELEDVCSMNEDVLVRTGLLCKYTAQRLRPTYKFFHKSFQEYTAGRRLSSLLKSREPEEVSKGNSYLKKMVSISDITSLYGNLLLYTCGSSTEATRAIMRHLAMVCEHGSLQGLSVTKRPLWRQESIQNLRNTTEQDVLKAINVNSFVECGINLFSESISKSELSQEFEAFFQGKSLYINSENIPDYLFDFFKYLPNCVSALDFVKLDFYGRATVSQDKTGENSSGVHTEGPSTYIPSRAVSLFFNWMQKFKTLEVTLRDISKLNKQDIKYLGKIFSSASNLKLYIKRCAAVAGRLSSVLRTCKNIHSLMVEASPLTTEDEQYITSVTDLQNLSIHDLHTQRLPGGLADSLGNLKNLLKLILDDIRLNEEDAKSLAEGLRNLKKMRLLHLTRLSDMGEGMDYIVKSLSEEPCDLQEMKLVDCCLTANSLKILAQNLHNLVKLSVLDMSENYLEKAGSEALQGLIGRLGVLEQLSALMLPWCWDAYISLPNLLKQLEGTPGLVKLGLKNWRLRDEEIRSFGEFLEMNPLRDLQQLDLAGHGVSSDGWLSFMDVFENLKQLVFFDFGTEEFLPDAALVRKLGQVLSKLTLLQEARLTGWELDDYDISVIKGTFKLVTA

Target ORF information:

RefSeq Version	NM_001309432.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309432.1

ORF Insert Sequence:

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ATCGGCAGGC TTGGCGTGCT AGAACAGCTA 
AGCGCGTTGA TGCTGCCTTG GTGCTGGGAT GCGTACATCA GTCTGCCCAA TCTGCTGAAG 
CAACTGGAGG GGACCCCAGG GCTTGTCAAA CTTGGATTGA AAAACTGGAG GCTCAGAGAC 
GAAGAGATTA GAAGTTTCGG TGAATTTTTG GAGATGAATC CTCTGAGAGA CTTGCAGCAG 
TTGGATTTAG CAGGACACGG TGTGAGCAGT GATGGATGGC TTTCTTTCAT GGATGTGTTT 
GAGAATCTTA AGCAGTTGGT ATTTTTTGAC TTCGGCACTG AAGAGTTCTT ACCAGACGCA 
GCGCTGGTGA GGAAACTCGG TCAAGTGTTA TCTAAGCTAA CTCTGCTGCA AGAGGCAAGG 
CTCACAGGCT GGGAGCTTGA TGACTATGAC ATAAGTGTTA TTAAAGGAAC CTTTAAACTA 
GTGACTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_008764491.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762713.1 Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa17097
Clone ID Related Accession (Same CDS sequence)	NM_001309432.1 , XM_008764491.2
Accession Version	XM_008764491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ATCGGCAGGC TTGGCGTGCT AGAACAGCTA 
AGCGCGTTGA TGCTGCCTTG GTGCTGGGAT GCGTACATCA GTCTGCCCAA TCTGCTGAAG 
CAACTGGAGG GGACCCCAGG GCTTGTCAAA CTTGGATTGA AAAACTGGAG GCTCAGAGAC 
GAAGAGATTA GAAGTTTCGG TGAATTTTTG GAGATGAATC CTCTGAGAGA CTTGCAGCAG 
TTGGATTTAG CAGGACACGG TGTGAGCAGT GATGGATGGC TTTCTTTCAT GGATGTGTTT 
GAGAATCTTA AGCAGTTGGT ATTTTTTGAC TTCGGCACTG AAGAGTTCTT ACCAGACGCA 
GCGCTGGTGA GGAAACTCGG TCAAGTGTTA TCTAAGCTAA CTCTGCTGCA AGAGGCAAGG 
CTCACAGGCT GGGAGCTTGA TGACTATGAC ATAAGTGTTA TTAAAGGAAC CTTTAAACTA 
GTGACTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762713.1
CDS	233..3304
Translation	MNFIKENSQALIQRMGIVVIKQICDDLFALNVLNGEEVAIICSHRVEQDAARDIVHMILKKGSAACNLFLKSLENWNYPVYQDLTGHSLFHQNLEEDLDVLAQSLKDLYNSPVFKNFFPLGEDIDIIFNLQITFTEPVLWRKDHRHHRVEQMTLGSLLEALKSPCLIEGESGKGKSTLLQKIAMLWASGMCPALNQFKLVFFIRLSSARGGLFETLYDQLVNIPDSISKPTFRALLLKLHKKVLFLLDAYNEFHPQNCPEIEALVKENHRFKNMVIVTTTTECLRHIRHVGALTVEVGDMTEDSARVLIREVLINELAEGLLFQMQESRCLRNLMRTPLFVVITCAIQMGSEEFQAHTQTMLFQTFYDLLIQKNRRRHSGGTSGDFVRSLDYCGDLALEGVFSHKFDFELEDVCSMNEDVLVRTGLLCKYTAQRLRPTYKFFHKSFQEYTAGRRLSSLLKSREPEEVSKGNSYLKKMVSISDITSLYGNLLLYTCGSSTEATRAIMRHLAMVCEHGSLQGLSVTKRPLWRQESIQNLRNTTEQDVLKAINVNSFVECGINLFSESISKSELSQEFEAFFQGKSLYINSENIPDYLFDFFKYLPNCVSALDFVKLDFYGRATVSQDKTGENSSGVHTEGPSTYIPSRAVSLFFNWMQKFKTLEVTLRDISKLNKQDIKYLGKIFSSASNLKLYIKRCAAVAGRLSSVLRTCKNIHSLMVEASPLTTEDEQYITSVTDLQNLSIHDLHTQRLPGGLADSLGNLKNLLKLILDDIRLNEEDAKSLAEGLRNLKKMRLLHLTRLSDMGEGMDYIVKSLSEEPCDLQEMKLVDCCLTANSLKILAQNLHNLVKLSVLDMSENYLEKAGSEALQGLIGRLGVLEQLSALMLPWCWDAYISLPNLLKQLEGTPGLVKLGLKNWRLRDEEIRSFGEFLEMNPLRDLQQLDLAGHGVSSDGWLSFMDVFENLKQLVFFDFGTEEFLPDAALVRKLGQVLSKLTLLQEARLTGWELDDYDISVIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_008764491.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764491.2

ORF Insert Sequence:

ATGAACTTTA TAAAGGAAAA CAGCCAAGCC CTTATTCAGA GGATGGGCAT AGTGGTTATA 
AAGCAAATCT GTGATGACCT ATTTGCATTG AACGTTCTCA ACGGGGAAGA AGTTGCCATC 
ATTTGCAGTC ATCGGGTGGA GCAGGACGCT GCACGAGACA TCGTCCATAT GATTTTGAAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGAA CTATCCTGTG 
TATCAGGACT TAACTGGACA CAGTCTTTTT CATCAGAACT TGGAAGAAGA CTTGGATGTT 
CTGGCCCAGA GTCTAAAAGA CTTATACAAC AGCCCTGTTT TTAAGAACTT CTTTCCTCTG 
GGTGAGGATA TCGACATCAT TTTCAATCTG CAGATCACCT TCACAGAACC TGTCTTGTGG 
AGGAAGGACC ATCGTCATCA CCGAGTAGAG CAGATGACCC TGGGCAGCCT GCTGGAGGCT 
CTGAAGAGTC CCTGCCTCAT TGAAGGGGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAA 
AAAATTGCCA TGCTCTGGGC CTCTGGGATG TGCCCAGCTC TGAACCAGTT CAAATTGGTC 
TTCTTCATCC GCCTGAGCAG TGCTAGGGGT GGCCTGTTTG AAACATTGTA TGATCAGCTC 
GTGAACATAC CTGACTCCAT CAGCAAACCA ACCTTCAGGG CTCTGCTGCT GAAGTTACAC 
AAGAAAGTCC TCTTTCTCCT CGATGCTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATCGAGGCCC TGGTAAAGGA AAACCATCGT TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCACAT CAGACACGTT GGCGCCCTGA CTGTGGAGGT GGGAGATATG 
ACCGAAGACA GCGCCCGAGT TCTCATCCGG GAAGTGCTGA TAAATGAACT GGCTGAAGGC 
TTGTTGTTCC AGATGCAGGA GTCCAGGTGC TTGAGAAATC TGATGAGGAC CCCTCTCTTC 
GTGGTGATAA CCTGTGCCAT CCAGATGGGC AGTGAGGAAT TCCAAGCTCA CACTCAAACC 
ATGCTCTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGGCGCAG ACACAGTGGA 
GGAACTTCAG GTGATTTTGT CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTGGAAGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTT GAGGATGTGT GCAGCATGAA TGAGGACGTC 
CTGGTGAGGA CGGGGCTCCT CTGTAAGTAC ACGGCTCAGA GGCTGAGGCC CACGTATAAA 
TTCTTTCATA AATCCTTTCA GGAGTACACA GCAGGTCGGA GACTCAGCAG TTTGTTGAAG 
TCCAGAGAGC CAGAGGAGGT GAGCAAGGGG AATAGCTACT TGAAGAAAAT GGTTTCCATC 
TCCGACATCA CATCCCTGTA TGGTAATCTG CTCCTGTACA CTTGTGGGTC ATCCACAGAA 
GCAACCAGGG CCATCATGAG GCACCTTGCA ATGGTATGTG AGCATGGCAG CCTACAAGGA 
CTTTCTGTCA CCAAGCGGCC TCTTTGGAGA CAGGAGTCAA TACAAAACCT GAGAAACACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAC GTAAATTCCT TTGTAGAGTG TGGTATCAAT 
TTATTCTCGG AGAGTATATC TAAATCAGAG CTGAGCCAAG AATTTGAAGC TTTCTTCCAA 
GGGAAAAGTT TATACATCAA CTCCGAGAAC ATCCCTGATT ACTTATTCGA CTTCTTTAAA 
TACTTGCCTA ATTGTGTAAG CGCACTGGAC TTCGTCAAGC TGGATTTCTA TGGAAGAGCG 
ACAGTGTCCC AGGACAAGAC GGGAGAGAAC AGCTCTGGAG TCCACACGGA AGGGCCCTCG 
ACCTACATTC CCAGTAGGGC TGTGTCTTTG TTCTTCAATT GGATGCAGAA ATTCAAGACT 
CTAGAGGTCA CACTCCGAGA TATTAGCAAG TTGAATAAAC AAGATATCAA ATACCTGGGG 
AAGATATTCA GCTCCGCCAG CAACCTCAAG TTGTACATCA AGAGGTGCGC AGCCGTGGCC 
GGAAGGCTCA GCTCAGTCCT CAGAACCTGC AAGAACATCC ACTCCCTCAT GGTGGAAGCT 
AGTCCCCTCA CCACGGAGGA TGAGCAGTAC ATCACGTCTG TGACAGACCT CCAGAACTTA 
AGCATTCACG ACCTCCACAC TCAACGGCTG CCAGGTGGTC TGGCTGACAG CTTGGGTAAT 
CTGAAGAACC TCCTGAAGCT CATCCTGGAT GACATTAGGC TGAACGAGGA AGATGCTAAA 
AGCCTAGCGG AAGGCCTGCG AAACCTGAAG AAGATGCGTT TACTTCATTT GACTCGTTTG 
TCTGACATGG GGGAGGGGAT GGACTACATA GTCAAGTCTC TCTCGGAAGA ACCCTGTGAT 
CTACAAGAGA TGAAGTTGGT GGACTGCTGT CTGACCGCAA ATTCTCTGAA AATTCTAGCA 
CAGAATCTTC ACAATTTGGT CAAGCTGAGC GTTCTTGATA TGTCAGAAAA TTATCTGGAA 
AAGGCCGGAA GTGAAGCTCT ACAGGGACTG ATCGGCAGGC TTGGCGTGCT AGAACAGCTA 
AGCGCGTTGA TGCTGCCTTG GTGCTGGGAT GCGTACATCA GTCTGCCCAA TCTGCTGAAG 
CAACTGGAGG GGACCCCAGG GCTTGTCAAA CTTGGATTGA AAAACTGGAG GCTCAGAGAC 
GAAGAGATTA GAAGTTTCGG TGAATTTTTG GAGATGAATC CTCTGAGAGA CTTGCAGCAG 
TTGGATTTAG CAGGACACGG TGTGAGCAGT GATGGATGGC TTTCTTTCAT GGATGTGTTT 
GAGAATCTTA AGCAGTTGGT ATTTTTTGAC TTCGGCACTG AAGAGTTCTT ACCAGACGCA 
GCGCTGGTGA GGAAACTCGG TCAAGTGTTA TCTAAGCTAA CTCTGCTGCA AGAGGCAAGG 
CTCACAGGCT GGGAGCTTGA TGACTATGAC ATAAGTGTTA TTAAAGGAAC CTTTAAACTA 
GTGACTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation.
Structure (London, England : 1993)23(12)2349-57(2015 Dec)
Diebolder CA,Halff EF,Koster AJ,Huizinga EG,Koning RI

Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization.
Science (New York, N.Y.)350(6259)404-9(2015 Oct)
Zhang L,Chen S,Ruan J,Wu J,Tong AB,Yin Q,Li Y,David L,Lu A,Wang WL,Marks C,Ouyang Q,Zhang X,Mao Y,Wu H

Structural and biochemical basis for induced self-propagation of NLRC4.
Science (New York, N.Y.)350(6259)399-404(2015 Oct)
Hu Z,Zhou Q,Zhang C,Fan S,Cheng W,Zhao Y,Shao F,Wang HW,Sui SF,Chai J

Phosphorylation of NLRC4 is critical for inflammasome activation.
Nature490(7421)539-42(2012 Oct)
Qu Y,Misaghi S,Izrael-Tomasevic A,Newton K,Gilmour LL,Lamkanfi M,Louie S,Kayagaki N,Liu J,K?m?ves L,Cupp JE,Arnott D,Monack D,Dixit VM

NLRC4-driven production of IL-1? discriminates between pathogenic and commensal bacteria and promotes host intestinal defense.
Nature immunology13(5)449-56(2012 May)
Franchi L,Kamada N,Nakamura Y,Burberry A,Kuffa P,Suzuki S,Shaw MH,Kim YG,N??ez G

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Nlrc4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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