gRNAs
The following Ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa61189 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017593310.1 | |
| Accession Version | XM_017593310.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | ubiquitin conjugation factor E4 B isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017448799.1 |
| CDS | 728..4756 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017593310.1 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa10519 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001271198.1 | |
| Accession Version | NM_001271198.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-30 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | ubiquitin conjugation factor E4 B | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07050466.1, AABR07050465.1, AABR07050464.1 and AABR07050463.1. On or before Sep 8, 2012 this sequence version replaced XM_001075491.3, XM_233679.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001258127.1 |
| CDS | 841..4362 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001271198.1 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa28895 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006239387.3 | |
| Accession Version | XM_006239387.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | ubiquitin conjugation factor E4 B isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006239449.1 |
| CDS | 729..4895 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006239387.3 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis. |
Structural and functional characterization of the monomeric U-box domain from E4B. |
The WldS gene delays axonal but not somatic degeneration in a rat glaucoma model. |
Mammalian E4 is required for cardiac development and maintenance of the nervous system. |
Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis. |
