Bnc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bnc2
Entrez Gene ID	298189
Full Name	basonuclin 2
General protein information	Preferred Names basonuclin 2 Names zinc finger protein basonuclin-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763776.2	XP_008761998.2	zinc finger protein basonuclin-2 isoform X4
XM_008763777.2	XP_008761999.2	zinc finger protein basonuclin-2 isoform X5
XM_017593257.1	XP_017448746.1	zinc finger protein basonuclin-2 isoform X2
XM_017593258.1	XP_017448747.1	zinc finger protein basonuclin-2 isoform X2
XM_008763775.2	XP_008761997.2	zinc finger protein basonuclin-2 isoform X1
XM_017593259.1	XP_017448748.1	zinc finger protein basonuclin-2 isoform X3
NM_001106666.1	NP_001100136.1	zinc finger protein basonuclin-2

Pathways from BioSystems

Homologies

Homo sapiens (human)	BNC2	NP_060107.3
Mus musculus (house mouse)	Bnc2	NP_766458.3
Gallus gallus (chicken)	BNC2	XP_003643044.2
Canis lupus familiaris (dog)	BNC2	XP_005626710.1
Xenopus tropicalis (tropical clawed frog)	bnc2	XP_002935715.2
Pan troglodytes (chimpanzee)	BNC2	XP_520498.2
	BNC2	XP_001110467.2
Bos taurus (cattle)	BNC2	XP_002689619.3
Rattus norvegicus (Norway rat)	Bnc2	NP_001100136.1

Gene Ontology
Bibliography

Related articles in PubMed

Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins.
Vanhoutteghem A, Maciejewski-Duval A, Bouche C, Delhomme B, Herv? F, Daubigney F, Soubigou G, Araki M, Araki K, Yamamura K, Djian P
Proceedings of the National Academy of Sciences of the United States of America106(34)14432-7(2009 Aug)

Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes.
Romano RA, Li H, Tummala R, Maul R, Sinha S
Genomics83(5)821-33(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bnc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bnc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa61155	XM_008763777.2 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa61151	XM_008763775.2 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa61152	XM_017593258.1 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa61152	XM_017593257.1 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa61153	XM_017593259.1 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa61154	XM_008763776.2 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa04899	NM_001106666.1 Latest version!	Rattus norvegicus basonuclin 2 (Bnc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bnc2 ( XM_008763777.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761999.2 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa61155
Clone ID Related Accession (Same CDS sequence)	XM_008763777.2
Accession Version	XM_008763777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGTTCCA 
CTGGCATGTG CTGGCAGAGT GCTGGGCGCA GACTTCTGCC CAAACCTGGA GGAGATAGAC 
CAGAGGCTGG AAGTCCAGGC CATTCGCTGC ACATTGGTGA ACTGCACGTG TGAGTGTTTC 
CAGCCTGGCA AGATTAACCT GCGGACCTGT GATCAGTGTA AACATGGCTG GGTGGCCCAT 
GCCTTGGATA AACTCAGCAC ACAGCACCTA TACCACCCCA CTCAAGTGGA GATTGTGCAG 
TCCAACGTAG TGTTTGACAT CAGCAGCTTG ATGCTCTACG GGACGCAGGC AGTGCCTGTG 
CGGCTAAAGA TACTGCTAGA CCGGCTCTTC AGTGTCCTGA AGCAAGAGGA GGTGCTGCAC 
ATTCTCCACG GCCTGGGCTG GACCCTGCGG GACTATGTCC GAGGATACAT CCTTCAGGAC 
GCTGCTGGCA AAGTGCTGGA CCGCTGGGCC ATCATGTCTC GGGAAGAGGA AATCATCACC 
CTTCAGCAGT TTCTGCGGTT TGGGGAAACC AAATCCATCG TGGAGCTTAT GGCCATTCAG 
GAGAAAGAAG GGCAAGCTGT GGCCGTGCCG TCTTCAAAGA CAGACTCGGA TATAAGGACT 
TTCATCGAGA GCAATAATCG CACCAGGAGC CCTAGCCTCC TCGCCCACTT AGAGAACAGC 
AATCCTTCTA GCATTCATCA TTTTGAAAAC ATACCGAACA GCCTTGCATT TCTGCTTCCG 
TTCCAGTATA TAAACCCTGT CTCTGCCCCA CTGCTAGGGT TGCCACCCAA CGGGCTGCTT 
TTAGAGCAGC CCGGACTGAG ACTGAGGGAA CCGAGCATTT CAACCCAGAA TGAGTACAAC 
GAGAGCAGTG AGTCTGAAGT ATCGCCTACA CCATATAAGA GTGACCAGAC GCCCAATAGA 
AATGCCCTGA CTAGCATCAC TAATGTGGAA CCCAAAACTG AGCCAGCGTG TGTCTCCCCC 
ATCCAGAATT CTGCCCCAGT CAGTGATCTA AGCAAACCTG AACACCCAAA AAGTTCATTC 
CGGATTCACC GTATGAGAAG GATGGGGTCA GCCTCGAGGA AAGGAAGGGT TTTCTGTAAT 
GCCTGTGGGA AGACATTCTA TGACAAAGGC ACTCTCAAAA TCCACTACAA TGCTGTTCAC 
CTGAAGATTA AACATCGATG TACCATTGAA GGCTGCAACA TGGTCTTTAG TTCCCTTCGA 
AGCCGGAATC GTCACAGTGC AAACCCCAAC CCCCGCCTTC ATATGCCTAT GCTAAGGAAT 
AACCGAGACA AAGATTTAAT TCGGGCTACA TCAGGAGCCG CCACTCCAGT CATAGCAAGT 
ACAAAATCAA ATCTCACACT CACGAGTCCT GGCCGGCCCC CGATGGGTTT TACCACTCCT 
CCACTAGACC CCGTCTTACA GAACCCTCTC CCAAGCCAGC TGGTATTTTC TGGGCTAAAG 
ACTGTACAGC CAGTTCCTCC TTTTTATAGA AGTTTACTTA CGCCAGGGGA AATGGTGAGT 
CCTCCAACCT CTCTCCCAAC CAGTCCCATC ATTCCAACCA GTGGCACCAT AGAGCAGCAC 
CCTCCTCCAC CCTCTGAGCC AATAGTGCCA GCAGTGATGA TGGGTACTCA CGAGCCCGGT 
GCTGACCTGG CACCTAAGAA AAAGCCTAGG AAGTCAAGCA TGCCTGTGAA AATTGAGAAG 
GAAATTATCG ATACTGCTGA CGAGTTCGAT GATGAGGACG ATGATCCTAA TGATGGTGGG 
ACCGTGGTCA ACGACATGAG TCACGGCAAT CATTGTCATT CCCAAGACGA GATGAGCCCA 
GGCATGTCGG TGAAGGACTT CTCTAAGCAT AACAGGACCC GGTGCATTTC AAGAACTGAA 
ATAAGAAGGG CTGACAGTAT GACATCTGAG GACCAGGAGC CTGAGCGGGA CTATGAGAAT 
GAGTCCGAGT CTTCAGAGCC TAAACTCGGT GAGGAGTCCA TGGTAGAAGA TGAGCACATT 
CACAGTGAGG TGAGCGACAA AGTCCTGATG AACAGTGAAC GAGCCGATGA GAACCACAGT 
GAGCCCTCGC ACCAGGATGT CATCAAGGTC AAGGAGGAAT TTACAGACCC CACTTACGAC 
ATGTTTTACA TGAGCCAGTA CGGACTATAC AATGGCGGGG GGGCCAGCAT GGCTGCCCTG 
CATGATAGTT TTACATCGTC TCTGAATTAT GGAAGCCCTC AAAAATTCTC CCCTGAAGGT 
GACCTGTGTT CTAGTCCAGA CCCCAAAATC TGTTACGTGT GCAAGAAGAG CTTCAAAAGC 
TCCTACAGCG TGAAGCTTCA CTACAGGAAC GTTCACTTAA AAGAAATGCA TGTCTGCACA 
GTGGCTGGCT GCAATGCTGC CTTCCCCTCT CGTCGAAGCA GAGACAGCAA AAGATTAGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761999.2
CDS	2973..5738
Translation	MVLTNCSNTAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEIDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDSKRLES

Target ORF information:

RefSeq Version	XM_008763777.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763777.2

ORF Insert Sequence:

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGTTCCA 
CTGGCATGTG CTGGCAGAGT GCTGGGCGCA GACTTCTGCC CAAACCTGGA GGAGATAGAC 
CAGAGGCTGG AAGTCCAGGC CATTCGCTGC ACATTGGTGA ACTGCACGTG TGAGTGTTTC 
CAGCCTGGCA AGATTAACCT GCGGACCTGT GATCAGTGTA AACATGGCTG GGTGGCCCAT 
GCCTTGGATA AACTCAGCAC ACAGCACCTA TACCACCCCA CTCAAGTGGA GATTGTGCAG 
TCCAACGTAG TGTTTGACAT CAGCAGCTTG ATGCTCTACG GGACGCAGGC AGTGCCTGTG 
CGGCTAAAGA TACTGCTAGA CCGGCTCTTC AGTGTCCTGA AGCAAGAGGA GGTGCTGCAC 
ATTCTCCACG GCCTGGGCTG GACCCTGCGG GACTATGTCC GAGGATACAT CCTTCAGGAC 
GCTGCTGGCA AAGTGCTGGA CCGCTGGGCC ATCATGTCTC GGGAAGAGGA AATCATCACC 
CTTCAGCAGT TTCTGCGGTT TGGGGAAACC AAATCCATCG TGGAGCTTAT GGCCATTCAG 
GAGAAAGAAG GGCAAGCTGT GGCCGTGCCG TCTTCAAAGA CAGACTCGGA TATAAGGACT 
TTCATCGAGA GCAATAATCG CACCAGGAGC CCTAGCCTCC TCGCCCACTT AGAGAACAGC 
AATCCTTCTA GCATTCATCA TTTTGAAAAC ATACCGAACA GCCTTGCATT TCTGCTTCCG 
TTCCAGTATA TAAACCCTGT CTCTGCCCCA CTGCTAGGGT TGCCACCCAA CGGGCTGCTT 
TTAGAGCAGC CCGGACTGAG ACTGAGGGAA CCGAGCATTT CAACCCAGAA TGAGTACAAC 
GAGAGCAGTG AGTCTGAAGT ATCGCCTACA CCATATAAGA GTGACCAGAC GCCCAATAGA 
AATGCCCTGA CTAGCATCAC TAATGTGGAA CCCAAAACTG AGCCAGCGTG TGTCTCCCCC 
ATCCAGAATT CTGCCCCAGT CAGTGATCTA AGCAAACCTG AACACCCAAA AAGTTCATTC 
CGGATTCACC GTATGAGAAG GATGGGGTCA GCCTCGAGGA AAGGAAGGGT TTTCTGTAAT 
GCCTGTGGGA AGACATTCTA TGACAAAGGC ACTCTCAAAA TCCACTACAA TGCTGTTCAC 
CTGAAGATTA AACATCGATG TACCATTGAA GGCTGCAACA TGGTCTTTAG TTCCCTTCGA 
AGCCGGAATC GTCACAGTGC AAACCCCAAC CCCCGCCTTC ATATGCCTAT GCTAAGGAAT 
AACCGAGACA AAGATTTAAT TCGGGCTACA TCAGGAGCCG CCACTCCAGT CATAGCAAGT 
ACAAAATCAA ATCTCACACT CACGAGTCCT GGCCGGCCCC CGATGGGTTT TACCACTCCT 
CCACTAGACC CCGTCTTACA GAACCCTCTC CCAAGCCAGC TGGTATTTTC TGGGCTAAAG 
ACTGTACAGC CAGTTCCTCC TTTTTATAGA AGTTTACTTA CGCCAGGGGA AATGGTGAGT 
CCTCCAACCT CTCTCCCAAC CAGTCCCATC ATTCCAACCA GTGGCACCAT AGAGCAGCAC 
CCTCCTCCAC CCTCTGAGCC AATAGTGCCA GCAGTGATGA TGGGTACTCA CGAGCCCGGT 
GCTGACCTGG CACCTAAGAA AAAGCCTAGG AAGTCAAGCA TGCCTGTGAA AATTGAGAAG 
GAAATTATCG ATACTGCTGA CGAGTTCGAT GATGAGGACG ATGATCCTAA TGATGGTGGG 
ACCGTGGTCA ACGACATGAG TCACGGCAAT CATTGTCATT CCCAAGACGA GATGAGCCCA 
GGCATGTCGG TGAAGGACTT CTCTAAGCAT AACAGGACCC GGTGCATTTC AAGAACTGAA 
ATAAGAAGGG CTGACAGTAT GACATCTGAG GACCAGGAGC CTGAGCGGGA CTATGAGAAT 
GAGTCCGAGT CTTCAGAGCC TAAACTCGGT GAGGAGTCCA TGGTAGAAGA TGAGCACATT 
CACAGTGAGG TGAGCGACAA AGTCCTGATG AACAGTGAAC GAGCCGATGA GAACCACAGT 
GAGCCCTCGC ACCAGGATGT CATCAAGGTC AAGGAGGAAT TTACAGACCC CACTTACGAC 
ATGTTTTACA TGAGCCAGTA CGGACTATAC AATGGCGGGG GGGCCAGCAT GGCTGCCCTG 
CATGATAGTT TTACATCGTC TCTGAATTAT GGAAGCCCTC AAAAATTCTC CCCTGAAGGT 
GACCTGTGTT CTAGTCCAGA CCCCAAAATC TGTTACGTGT GCAAGAAGAG CTTCAAAAGC 
TCCTACAGCG TGAAGCTTCA CTACAGGAAC GTTCACTTAA AAGAAATGCA TGTCTGCACA 
GTGGCTGGCT GCAATGCTGC CTTCCCCTCT CGTCGAAGCA GAGACAGCAA AAGATTAGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( XM_008763775.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761997.2 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61151
Clone ID Related Accession (Same CDS sequence)	XM_008763775.2
Accession Version	XM_008763775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGTTCCA 
CTGGCATGTG CTGGCAGAGT GCTGGGCGCA GACTTCTGCC CAAACCTGGA GGAGATAGAC 
CAGAGGCTGG AAGTCCAGGC CATTCGCTGC ACATTGGTGA ACTGCACGTG TGAGTGTTTC 
CAGCCTGGCA AGATTAACCT GCGGACCTGT GATCAGTGTA AACATGGCTG GGTGGCCCAT 
GCCTTGGATA AACTCAGCAC ACAGCACCTA TACCACCCCA CTCAAGTGGA GATTGTGCAG 
TCCAACGTAG TGTTTGACAT CAGCAGCTTG ATGCTCTACG GGACGCAGGC AGTGCCTGTG 
CGGCTAAAGA TACTGCTAGA CCGGCTCTTC AGTGTCCTGA AGCAAGAGGA GGTGCTGCAC 
ATTCTCCACG GCCTGGGCTG GACCCTGCGG GACTATGTCC GAGGATACAT CCTTCAGGAC 
GCTGCTGGCA AAGTGCTGGA CCGCTGGGCC ATCATGTCTC GGGAAGAGGA AATCATCACC 
CTTCAGCAGT TTCTGCGGTT TGGGGAAACC AAATCCATCG TGGAGCTTAT GGCCATTCAG 
GAGAAAGAAG GGCAAGCTGT GGCCGTGCCG TCTTCAAAGA CAGACTCGGA TATAAGGACT 
TTCATCGAGA GCAATAATCG CACCAGGAGC CCTAGCCTCC TCGCCCACTT AGAGAACAGC 
AATCCTTCTA GCATTCATCA TTTTGAAAAC ATACCGAACA GCCTTGCATT TCTGCTTCCG 
TTCCAGTATA TAAACCCTGT CTCTGCCCCA CTGCTAGGGT TGCCACCCAA CGGGCTGCTT 
TTAGAGCAGC CCGGACTGAG ACTGAGGGAA CCGAGCATTT CAACCCAGAA TGAGTACAAC 
GAGAGCAGTG AGTCTGAAGT ATCGCCTACA CCATATAAGA GTGACCAGAC GCCCAATAGA 
AATGCCCTGA CTAGCATCAC TAATGTGGAA CCCAAAACTG AGCCAGCGTG TGTCTCCCCC 
ATCCAGAATT CTGCCCCAGT CAGTGATCTA AGCAAACCTG AACACCCAAA AAGTTCATTC 
CGGATTCACC GTATGAGAAG GATGGGGTCA GCCTCGAGGA AAGGAAGGGT TTTCTGTAAT 
GCCTGTGGGA AGACATTCTA TGACAAAGGC ACTCTCAAAA TCCACTACAA TGCTGTTCAC 
CTGAAGATTA AACATCGATG TACCATTGAA GGCTGCAACA TGGTCTTTAG TTCCCTTCGA 
AGCCGGAATC GTCACAGTGC AAACCCCAAC CCCCGCCTTC ATATGCCTAT GCTAAGGAAT 
AACCGAGACA AAGATTTAAT TCGGGCTACA TCAGGAGCCG CCACTCCAGT CATAGCAAGT 
ACAAAATCAA ATCTCACACT CACGAGTCCT GGCCGGCCCC CGATGGGTTT TACCACTCCT 
CCACTAGACC CCGTCTTACA GAACCCTCTC CCAAGCCAGC TGGTATTTTC TGGGCTAAAG 
ACTGTACAGC CAGTTCCTCC TTTTTATAGA AGTTTACTTA CGCCAGGGGA AATGGTGAGT 
CCTCCAACCT CTCTCCCAAC CAGTCCCATC ATTCCAACCA GTGGCACCAT AGAGCAGCAC 
CCTCCTCCAC CCTCTGAGCC AATAGTGCCA GCAGTGATGA TGGGTACTCA CGAGCCCGGT 
GCTGACCTGG CACCTAAGAA AAAGCCTAGG AAGTCAAGCA TGCCTGTGAA AATTGAGAAG 
GAAATTATCG ATACTGCTGA CGAGTTCGAT GATGAGGACG ATGATCCTAA TGATGGTGGG 
ACCGTGGTCA ACGACATGAG TCACGGCAAT CATTGTCATT CCCAAGACGA GATGAGCCCA 
GGCATGTCGG TGAAGGACTT CTCTAAGCAT AACAGGACCC GGTGCATTTC AAGAACTGAA 
ATAAGAAGGG CTGACAGTAT GACATCTGAG GACCAGGAGC CTGAGCGGGA CTATGAGAAT 
GAGTCCGAGT CTTCAGAGCC TAAACTCGGT GAGGAGTCCA TGGTAGAAGA TGAGCACATT 
CACAGTGAGG TGAGCGACAA AGTCCTGATG AACAGTGAAC GAGCCGATGA GAACCACAGT 
GAGCCCTCGC ACCAGGATGT CATCAAGGTC AAGGAGGAAT TTACAGACCC CACTTACGAC 
ATGTTTTACA TGAGCCAGTA CGGACTATAC AATGGCGGGG GGGCCAGCAT GGCTGCCCTG 
CATGATAGTT TTACATCGTC TCTGAATTAT GGAAGCCCTC AAAAATTCTC CCCTGAAGGT 
GACCTGTGTT CTAGTCCAGA CCCCAAAATC TGTTACGTGT GCAAGAAGAG CTTCAAAAGC 
TCCTACAGCG TGAAGCTTCA CTACAGGAAC GTTCACTTAA AAGAAATGCA TGTCTGCACA 
GTGGCTGGCT GCAATGCTGC CTTCCCCTCT CGTCGAAGCA GAGACAGACA CAGTGCCAAC 
ATAAACCTGC ATCGTAAACT GTTGACCAAA GAACTCGATG ACATGGGCCT GGACTCATCG 
CAGCCTTCCC TTAGCAAGGA CCTCCGGGAT GAATTTTTGA TGAAGATCTA TGGTACCCAG 
CACCCTCTGG GGCTCGATGT CAGGGAAGAT GCCTCCTCTC CCGCAGGGAC GGAAGACTCC 
CACCTGAATG GGTATGGGAG GGGCATGGCA GAGGACTACA TGGTCCTTGA CCTGAGCACC 
ACCTCCAGCC TCCAGTCCAG CAGCAGTATC CATTCCTCCA GAGAATCCGA TGCAGGCAGC 
GACGAGGGGA TTCTTCTTGA CGACATTGAT GGGGCGAGTG ACAGTGGGGA GTCCGCTCAC 
AAGGCTGAGG CCCCCACCCT CCCCAGCAGC CTTGGGGCCG AAGTTTCAGG ATCTCTGATG 
TTCAGCAGTT TGTCTGGGAG CAATGGTGGG ATCATGTGCA ACATTTGCCA CAAAATGTAC 
AGCAACAAGG GGACCCTGAG GGTCCACTAC AAAACTGTGC ATTTGCGAGA GATGCACAAG 
TGCAAAGTCC CCGGCTGTAA CATGATGTTC TCCTCTGTGC GAAGCCGAAA CAGGCACAGT 
CAGAACCCTA ATCTCCACAA AAACATTCCC TTCACTTCAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761997.2
CDS	2973..6380
Translation	MVLTNCSNTAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEIDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	XM_008763775.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763775.2

ORF Insert Sequence:

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGTTCCA 
CTGGCATGTG CTGGCAGAGT GCTGGGCGCA GACTTCTGCC CAAACCTGGA GGAGATAGAC 
CAGAGGCTGG AAGTCCAGGC CATTCGCTGC ACATTGGTGA ACTGCACGTG TGAGTGTTTC 
CAGCCTGGCA AGATTAACCT GCGGACCTGT GATCAGTGTA AACATGGCTG GGTGGCCCAT 
GCCTTGGATA AACTCAGCAC ACAGCACCTA TACCACCCCA CTCAAGTGGA GATTGTGCAG 
TCCAACGTAG TGTTTGACAT CAGCAGCTTG ATGCTCTACG GGACGCAGGC AGTGCCTGTG 
CGGCTAAAGA TACTGCTAGA CCGGCTCTTC AGTGTCCTGA AGCAAGAGGA GGTGCTGCAC 
ATTCTCCACG GCCTGGGCTG GACCCTGCGG GACTATGTCC GAGGATACAT CCTTCAGGAC 
GCTGCTGGCA AAGTGCTGGA CCGCTGGGCC ATCATGTCTC GGGAAGAGGA AATCATCACC 
CTTCAGCAGT TTCTGCGGTT TGGGGAAACC AAATCCATCG TGGAGCTTAT GGCCATTCAG 
GAGAAAGAAG GGCAAGCTGT GGCCGTGCCG TCTTCAAAGA CAGACTCGGA TATAAGGACT 
TTCATCGAGA GCAATAATCG CACCAGGAGC CCTAGCCTCC TCGCCCACTT AGAGAACAGC 
AATCCTTCTA GCATTCATCA TTTTGAAAAC ATACCGAACA GCCTTGCATT TCTGCTTCCG 
TTCCAGTATA TAAACCCTGT CTCTGCCCCA CTGCTAGGGT TGCCACCCAA CGGGCTGCTT 
TTAGAGCAGC CCGGACTGAG ACTGAGGGAA CCGAGCATTT CAACCCAGAA TGAGTACAAC 
GAGAGCAGTG AGTCTGAAGT ATCGCCTACA CCATATAAGA GTGACCAGAC GCCCAATAGA 
AATGCCCTGA CTAGCATCAC TAATGTGGAA CCCAAAACTG AGCCAGCGTG TGTCTCCCCC 
ATCCAGAATT CTGCCCCAGT CAGTGATCTA AGCAAACCTG AACACCCAAA AAGTTCATTC 
CGGATTCACC GTATGAGAAG GATGGGGTCA GCCTCGAGGA AAGGAAGGGT TTTCTGTAAT 
GCCTGTGGGA AGACATTCTA TGACAAAGGC ACTCTCAAAA TCCACTACAA TGCTGTTCAC 
CTGAAGATTA AACATCGATG TACCATTGAA GGCTGCAACA TGGTCTTTAG TTCCCTTCGA 
AGCCGGAATC GTCACAGTGC AAACCCCAAC CCCCGCCTTC ATATGCCTAT GCTAAGGAAT 
AACCGAGACA AAGATTTAAT TCGGGCTACA TCAGGAGCCG CCACTCCAGT CATAGCAAGT 
ACAAAATCAA ATCTCACACT CACGAGTCCT GGCCGGCCCC CGATGGGTTT TACCACTCCT 
CCACTAGACC CCGTCTTACA GAACCCTCTC CCAAGCCAGC TGGTATTTTC TGGGCTAAAG 
ACTGTACAGC CAGTTCCTCC TTTTTATAGA AGTTTACTTA CGCCAGGGGA AATGGTGAGT 
CCTCCAACCT CTCTCCCAAC CAGTCCCATC ATTCCAACCA GTGGCACCAT AGAGCAGCAC 
CCTCCTCCAC CCTCTGAGCC AATAGTGCCA GCAGTGATGA TGGGTACTCA CGAGCCCGGT 
GCTGACCTGG CACCTAAGAA AAAGCCTAGG AAGTCAAGCA TGCCTGTGAA AATTGAGAAG 
GAAATTATCG ATACTGCTGA CGAGTTCGAT GATGAGGACG ATGATCCTAA TGATGGTGGG 
ACCGTGGTCA ACGACATGAG TCACGGCAAT CATTGTCATT CCCAAGACGA GATGAGCCCA 
GGCATGTCGG TGAAGGACTT CTCTAAGCAT AACAGGACCC GGTGCATTTC AAGAACTGAA 
ATAAGAAGGG CTGACAGTAT GACATCTGAG GACCAGGAGC CTGAGCGGGA CTATGAGAAT 
GAGTCCGAGT CTTCAGAGCC TAAACTCGGT GAGGAGTCCA TGGTAGAAGA TGAGCACATT 
CACAGTGAGG TGAGCGACAA AGTCCTGATG AACAGTGAAC GAGCCGATGA GAACCACAGT 
GAGCCCTCGC ACCAGGATGT CATCAAGGTC AAGGAGGAAT TTACAGACCC CACTTACGAC 
ATGTTTTACA TGAGCCAGTA CGGACTATAC AATGGCGGGG GGGCCAGCAT GGCTGCCCTG 
CATGATAGTT TTACATCGTC TCTGAATTAT GGAAGCCCTC AAAAATTCTC CCCTGAAGGT 
GACCTGTGTT CTAGTCCAGA CCCCAAAATC TGTTACGTGT GCAAGAAGAG CTTCAAAAGC 
TCCTACAGCG TGAAGCTTCA CTACAGGAAC GTTCACTTAA AAGAAATGCA TGTCTGCACA 
GTGGCTGGCT GCAATGCTGC CTTCCCCTCT CGTCGAAGCA GAGACAGACA CAGTGCCAAC 
ATAAACCTGC ATCGTAAACT GTTGACCAAA GAACTCGATG ACATGGGCCT GGACTCATCG 
CAGCCTTCCC TTAGCAAGGA CCTCCGGGAT GAATTTTTGA TGAAGATCTA TGGTACCCAG 
CACCCTCTGG GGCTCGATGT CAGGGAAGAT GCCTCCTCTC CCGCAGGGAC GGAAGACTCC 
CACCTGAATG GGTATGGGAG GGGCATGGCA GAGGACTACA TGGTCCTTGA CCTGAGCACC 
ACCTCCAGCC TCCAGTCCAG CAGCAGTATC CATTCCTCCA GAGAATCCGA TGCAGGCAGC 
GACGAGGGGA TTCTTCTTGA CGACATTGAT GGGGCGAGTG ACAGTGGGGA GTCCGCTCAC 
AAGGCTGAGG CCCCCACCCT CCCCAGCAGC CTTGGGGCCG AAGTTTCAGG ATCTCTGATG 
TTCAGCAGTT TGTCTGGGAG CAATGGTGGG ATCATGTGCA ACATTTGCCA CAAAATGTAC 
AGCAACAAGG GGACCCTGAG GGTCCACTAC AAAACTGTGC ATTTGCGAGA GATGCACAAG 
TGCAAAGTCC CCGGCTGTAA CATGATGTTC TCCTCTGTGC GAAGCCGAAA CAGGCACAGT 
CAGAACCCTA ATCTCCACAA AAACATTCCC TTCACTTCAA TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( XM_017593257.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448746.1 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X2, mRNA.

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CloneID	ORa61152
Clone ID Related Accession (Same CDS sequence)	XM_017593257.1 , XM_017593258.1
Accession Version	XM_017593257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGTTCCACTG 
GCATGTGCTG GCAGAGTGCT GGGCGCAGAC TTCTGCCCAA ACCTGGAGGA GATAGACCAG 
AGGCTGGAAG TCCAGGCCAT TCGCTGCACA TTGGTGAACT GCACGTGTGA GTGTTTCCAG 
CCTGGCAAGA TTAACCTGCG GACCTGTGAT CAGTGTAAAC ATGGCTGGGT GGCCCATGCC 
TTGGATAAAC TCAGCACACA GCACCTATAC CACCCCACTC AAGTGGAGAT TGTGCAGTCC 
AACGTAGTGT TTGACATCAG CAGCTTGATG CTCTACGGGA CGCAGGCAGT GCCTGTGCGG 
CTAAAGATAC TGCTAGACCG GCTCTTCAGT GTCCTGAAGC AAGAGGAGGT GCTGCACATT 
CTCCACGGCC TGGGCTGGAC CCTGCGGGAC TATGTCCGAG GATACATCCT TCAGGACGCT 
GCTGGCAAAG TGCTGGACCG CTGGGCCATC ATGTCTCGGG AAGAGGAAAT CATCACCCTT 
CAGCAGTTTC TGCGGTTTGG GGAAACCAAA TCCATCGTGG AGCTTATGGC CATTCAGGAG 
AAAGAAGGGC AAGCTGTGGC CGTGCCGTCT TCAAAGACAG ACTCGGATAT AAGGACTTTC 
ATCGAGAGCA ATAATCGCAC CAGGAGCCCT AGCCTCCTCG CCCACTTAGA GAACAGCAAT 
CCTTCTAGCA TTCATCATTT TGAAAACATA CCGAACAGCC TTGCATTTCT GCTTCCGTTC 
CAGTATATAA ACCCTGTCTC TGCCCCACTG CTAGGGTTGC CACCCAACGG GCTGCTTTTA 
GAGCAGCCCG GACTGAGACT GAGGGAACCG AGCATTTCAA CCCAGAATGA GTACAACGAG 
AGCAGTGAGT CTGAAGTATC GCCTACACCA TATAAGAGTG ACCAGACGCC CAATAGAAAT 
GCCCTGACTA GCATCACTAA TGTGGAACCC AAAACTGAGC CAGCGTGTGT CTCCCCCATC 
CAGAATTCTG CCCCAGTCAG TGATCTAAGC AAACCTGAAC ACCCAAAAAG TTCATTCCGG 
ATTCACCGTA TGAGAAGGAT GGGGTCAGCC TCGAGGAAAG GAAGGGTTTT CTGTAATGCC 
TGTGGGAAGA CATTCTATGA CAAAGGCACT CTCAAAATCC ACTACAATGC TGTTCACCTG 
AAGATTAAAC ATCGATGTAC CATTGAAGGC TGCAACATGG TCTTTAGTTC CCTTCGAAGC 
CGGAATCGTC ACAGTGCAAA CCCCAACCCC CGCCTTCATA TGCCTATGCT AAGGAATAAC 
CGAGACAAAG ATTTAATTCG GGCTACATCA GGAGCCGCCA CTCCAGTCAT AGCAAGTACA 
AAATCAAATC TCACACTCAC GAGTCCTGGC CGGCCCCCGA TGGGTTTTAC CACTCCTCCA 
CTAGACCCCG TCTTACAGAA CCCTCTCCCA AGCCAGCTGG TATTTTCTGG GCTAAAGACT 
GTACAGCCAG TTCCTCCTTT TTATAGAAGT TTACTTACGC CAGGGGAAAT GGTGAGTCCT 
CCAACCTCTC TCCCAACCAG TCCCATCATT CCAACCAGTG GCACCATAGA GCAGCACCCT 
CCTCCACCCT CTGAGCCAAT AGTGCCAGCA GTGATGATGG GTACTCACGA GCCCGGTGCT 
GACCTGGCAC CTAAGAAAAA GCCTAGGAAG TCAAGCATGC CTGTGAAAAT TGAGAAGGAA 
ATTATCGATA CTGCTGACGA GTTCGATGAT GAGGACGATG ATCCTAATGA TGGTGGGACC 
GTGGTCAACG ACATGAGTCA CGGCAATCAT TGTCATTCCC AAGACGAGAT GAGCCCAGGC 
ATGTCGGTGA AGGACTTCTC TAAGCATAAC AGGACCCGGT GCATTTCAAG AACTGAAATA 
AGAAGGGCTG ACAGTATGAC ATCTGAGGAC CAGGAGCCTG AGCGGGACTA TGAGAATGAG 
TCCGAGTCTT CAGAGCCTAA ACTCGGTGAG GAGTCCATGG TAGAAGATGA GCACATTCAC 
AGTGAGGTGA GCGACAAAGT CCTGATGAAC AGTGAACGAG CCGATGAGAA CCACAGTGAG 
CCCTCGCACC AGGATGTCAT CAAGGTCAAG GAGGAATTTA CAGACCCCAC TTACGACATG 
TTTTACATGA GCCAGTACGG ACTATACAAT GGCGGGGGGG CCAGCATGGC TGCCCTGCAT 
GATAGTTTTA CATCGTCTCT GAATTATGGA AGCCCTCAAA AATTCTCCCC TGAAGGTGAC 
CTGTGTTCTA GTCCAGACCC CAAAATCTGT TACGTGTGCA AGAAGAGCTT CAAAAGCTCC 
TACAGCGTGA AGCTTCACTA CAGGAACGTT CACTTAAAAG AAATGCATGT CTGCACAGTG 
GCTGGCTGCA ATGCTGCCTT CCCCTCTCGT CGAAGCAGAG ACAGACACAG TGCCAACATA 
AACCTGCATC GTAAACTGTT GACCAAAGAA CTCGATGACA TGGGCCTGGA CTCATCGCAG 
CCTTCCCTTA GCAAGGACCT CCGGGATGAA TTTTTGATGA AGATCTATGG TACCCAGCAC 
CCTCTGGGGC TCGATGTCAG GGAAGATGCC TCCTCTCCCG CAGGGACGGA AGACTCCCAC 
CTGAATGGGT ATGGGAGGGG CATGGCAGAG GACTACATGG TCCTTGACCT GAGCACCACC 
TCCAGCCTCC AGTCCAGCAG CAGTATCCAT TCCTCCAGAG AATCCGATGC AGGCAGCGAC 
GAGGGGATTC TTCTTGACGA CATTGATGGG GCGAGTGACA GTGGGGAGTC CGCTCACAAG 
GCTGAGGCCC CCACCCTCCC CAGCAGCCTT GGGGCCGAAG TTTCAGGATC TCTGATGTTC 
AGCAGTTTGT CTGGGAGCAA TGGTGGGATC ATGTGCAACA TTTGCCACAA AATGTACAGC 
AACAAGGGGA CCCTGAGGGT CCACTACAAA ACTGTGCATT TGCGAGAGAT GCACAAGTGC 
AAAGTCCCCG GCTGTAACAT GATGTTCTCC TCTGTGCGAA GCCGAAACAG GCACAGTCAG 
AACCCTAATC TCCACAAAAA CATTCCCTTC ACTTCAATAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448746.1
CDS	8795..12199
Translation	MMDCRAWLAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEIDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	XM_017593257.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593257.1

ORF Insert Sequence:

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGTTCCACTG 
GCATGTGCTG GCAGAGTGCT GGGCGCAGAC TTCTGCCCAA ACCTGGAGGA GATAGACCAG 
AGGCTGGAAG TCCAGGCCAT TCGCTGCACA TTGGTGAACT GCACGTGTGA GTGTTTCCAG 
CCTGGCAAGA TTAACCTGCG GACCTGTGAT CAGTGTAAAC ATGGCTGGGT GGCCCATGCC 
TTGGATAAAC TCAGCACACA GCACCTATAC CACCCCACTC AAGTGGAGAT TGTGCAGTCC 
AACGTAGTGT TTGACATCAG CAGCTTGATG CTCTACGGGA CGCAGGCAGT GCCTGTGCGG 
CTAAAGATAC TGCTAGACCG GCTCTTCAGT GTCCTGAAGC AAGAGGAGGT GCTGCACATT 
CTCCACGGCC TGGGCTGGAC CCTGCGGGAC TATGTCCGAG GATACATCCT TCAGGACGCT 
GCTGGCAAAG TGCTGGACCG CTGGGCCATC ATGTCTCGGG AAGAGGAAAT CATCACCCTT 
CAGCAGTTTC TGCGGTTTGG GGAAACCAAA TCCATCGTGG AGCTTATGGC CATTCAGGAG 
AAAGAAGGGC AAGCTGTGGC CGTGCCGTCT TCAAAGACAG ACTCGGATAT AAGGACTTTC 
ATCGAGAGCA ATAATCGCAC CAGGAGCCCT AGCCTCCTCG CCCACTTAGA GAACAGCAAT 
CCTTCTAGCA TTCATCATTT TGAAAACATA CCGAACAGCC TTGCATTTCT GCTTCCGTTC 
CAGTATATAA ACCCTGTCTC TGCCCCACTG CTAGGGTTGC CACCCAACGG GCTGCTTTTA 
GAGCAGCCCG GACTGAGACT GAGGGAACCG AGCATTTCAA CCCAGAATGA GTACAACGAG 
AGCAGTGAGT CTGAAGTATC GCCTACACCA TATAAGAGTG ACCAGACGCC CAATAGAAAT 
GCCCTGACTA GCATCACTAA TGTGGAACCC AAAACTGAGC CAGCGTGTGT CTCCCCCATC 
CAGAATTCTG CCCCAGTCAG TGATCTAAGC AAACCTGAAC ACCCAAAAAG TTCATTCCGG 
ATTCACCGTA TGAGAAGGAT GGGGTCAGCC TCGAGGAAAG GAAGGGTTTT CTGTAATGCC 
TGTGGGAAGA CATTCTATGA CAAAGGCACT CTCAAAATCC ACTACAATGC TGTTCACCTG 
AAGATTAAAC ATCGATGTAC CATTGAAGGC TGCAACATGG TCTTTAGTTC CCTTCGAAGC 
CGGAATCGTC ACAGTGCAAA CCCCAACCCC CGCCTTCATA TGCCTATGCT AAGGAATAAC 
CGAGACAAAG ATTTAATTCG GGCTACATCA GGAGCCGCCA CTCCAGTCAT AGCAAGTACA 
AAATCAAATC TCACACTCAC GAGTCCTGGC CGGCCCCCGA TGGGTTTTAC CACTCCTCCA 
CTAGACCCCG TCTTACAGAA CCCTCTCCCA AGCCAGCTGG TATTTTCTGG GCTAAAGACT 
GTACAGCCAG TTCCTCCTTT TTATAGAAGT TTACTTACGC CAGGGGAAAT GGTGAGTCCT 
CCAACCTCTC TCCCAACCAG TCCCATCATT CCAACCAGTG GCACCATAGA GCAGCACCCT 
CCTCCACCCT CTGAGCCAAT AGTGCCAGCA GTGATGATGG GTACTCACGA GCCCGGTGCT 
GACCTGGCAC CTAAGAAAAA GCCTAGGAAG TCAAGCATGC CTGTGAAAAT TGAGAAGGAA 
ATTATCGATA CTGCTGACGA GTTCGATGAT GAGGACGATG ATCCTAATGA TGGTGGGACC 
GTGGTCAACG ACATGAGTCA CGGCAATCAT TGTCATTCCC AAGACGAGAT GAGCCCAGGC 
ATGTCGGTGA AGGACTTCTC TAAGCATAAC AGGACCCGGT GCATTTCAAG AACTGAAATA 
AGAAGGGCTG ACAGTATGAC ATCTGAGGAC CAGGAGCCTG AGCGGGACTA TGAGAATGAG 
TCCGAGTCTT CAGAGCCTAA ACTCGGTGAG GAGTCCATGG TAGAAGATGA GCACATTCAC 
AGTGAGGTGA GCGACAAAGT CCTGATGAAC AGTGAACGAG CCGATGAGAA CCACAGTGAG 
CCCTCGCACC AGGATGTCAT CAAGGTCAAG GAGGAATTTA CAGACCCCAC TTACGACATG 
TTTTACATGA GCCAGTACGG ACTATACAAT GGCGGGGGGG CCAGCATGGC TGCCCTGCAT 
GATAGTTTTA CATCGTCTCT GAATTATGGA AGCCCTCAAA AATTCTCCCC TGAAGGTGAC 
CTGTGTTCTA GTCCAGACCC CAAAATCTGT TACGTGTGCA AGAAGAGCTT CAAAAGCTCC 
TACAGCGTGA AGCTTCACTA CAGGAACGTT CACTTAAAAG AAATGCATGT CTGCACAGTG 
GCTGGCTGCA ATGCTGCCTT CCCCTCTCGT CGAAGCAGAG ACAGACACAG TGCCAACATA 
AACCTGCATC GTAAACTGTT GACCAAAGAA CTCGATGACA TGGGCCTGGA CTCATCGCAG 
CCTTCCCTTA GCAAGGACCT CCGGGATGAA TTTTTGATGA AGATCTATGG TACCCAGCAC 
CCTCTGGGGC TCGATGTCAG GGAAGATGCC TCCTCTCCCG CAGGGACGGA AGACTCCCAC 
CTGAATGGGT ATGGGAGGGG CATGGCAGAG GACTACATGG TCCTTGACCT GAGCACCACC 
TCCAGCCTCC AGTCCAGCAG CAGTATCCAT TCCTCCAGAG AATCCGATGC AGGCAGCGAC 
GAGGGGATTC TTCTTGACGA CATTGATGGG GCGAGTGACA GTGGGGAGTC CGCTCACAAG 
GCTGAGGCCC CCACCCTCCC CAGCAGCCTT GGGGCCGAAG TTTCAGGATC TCTGATGTTC 
AGCAGTTTGT CTGGGAGCAA TGGTGGGATC ATGTGCAACA TTTGCCACAA AATGTACAGC 
AACAAGGGGA CCCTGAGGGT CCACTACAAA ACTGTGCATT TGCGAGAGAT GCACAAGTGC 
AAAGTCCCCG GCTGTAACAT GATGTTCTCC TCTGTGCGAA GCCGAAACAG GCACAGTCAG 
AACCCTAATC TCCACAAAAA CATTCCCTTC ACTTCAATAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( XM_017593258.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448747.1 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa61152
Clone ID Related Accession (Same CDS sequence)	XM_017593257.1 , XM_017593258.1
Accession Version	XM_017593258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGTTCCACTG 
GCATGTGCTG GCAGAGTGCT GGGCGCAGAC TTCTGCCCAA ACCTGGAGGA GATAGACCAG 
AGGCTGGAAG TCCAGGCCAT TCGCTGCACA TTGGTGAACT GCACGTGTGA GTGTTTCCAG 
CCTGGCAAGA TTAACCTGCG GACCTGTGAT CAGTGTAAAC ATGGCTGGGT GGCCCATGCC 
TTGGATAAAC TCAGCACACA GCACCTATAC CACCCCACTC AAGTGGAGAT TGTGCAGTCC 
AACGTAGTGT TTGACATCAG CAGCTTGATG CTCTACGGGA CGCAGGCAGT GCCTGTGCGG 
CTAAAGATAC TGCTAGACCG GCTCTTCAGT GTCCTGAAGC AAGAGGAGGT GCTGCACATT 
CTCCACGGCC TGGGCTGGAC CCTGCGGGAC TATGTCCGAG GATACATCCT TCAGGACGCT 
GCTGGCAAAG TGCTGGACCG CTGGGCCATC ATGTCTCGGG AAGAGGAAAT CATCACCCTT 
CAGCAGTTTC TGCGGTTTGG GGAAACCAAA TCCATCGTGG AGCTTATGGC CATTCAGGAG 
AAAGAAGGGC AAGCTGTGGC CGTGCCGTCT TCAAAGACAG ACTCGGATAT AAGGACTTTC 
ATCGAGAGCA ATAATCGCAC CAGGAGCCCT AGCCTCCTCG CCCACTTAGA GAACAGCAAT 
CCTTCTAGCA TTCATCATTT TGAAAACATA CCGAACAGCC TTGCATTTCT GCTTCCGTTC 
CAGTATATAA ACCCTGTCTC TGCCCCACTG CTAGGGTTGC CACCCAACGG GCTGCTTTTA 
GAGCAGCCCG GACTGAGACT GAGGGAACCG AGCATTTCAA CCCAGAATGA GTACAACGAG 
AGCAGTGAGT CTGAAGTATC GCCTACACCA TATAAGAGTG ACCAGACGCC CAATAGAAAT 
GCCCTGACTA GCATCACTAA TGTGGAACCC AAAACTGAGC CAGCGTGTGT CTCCCCCATC 
CAGAATTCTG CCCCAGTCAG TGATCTAAGC AAACCTGAAC ACCCAAAAAG TTCATTCCGG 
ATTCACCGTA TGAGAAGGAT GGGGTCAGCC TCGAGGAAAG GAAGGGTTTT CTGTAATGCC 
TGTGGGAAGA CATTCTATGA CAAAGGCACT CTCAAAATCC ACTACAATGC TGTTCACCTG 
AAGATTAAAC ATCGATGTAC CATTGAAGGC TGCAACATGG TCTTTAGTTC CCTTCGAAGC 
CGGAATCGTC ACAGTGCAAA CCCCAACCCC CGCCTTCATA TGCCTATGCT AAGGAATAAC 
CGAGACAAAG ATTTAATTCG GGCTACATCA GGAGCCGCCA CTCCAGTCAT AGCAAGTACA 
AAATCAAATC TCACACTCAC GAGTCCTGGC CGGCCCCCGA TGGGTTTTAC CACTCCTCCA 
CTAGACCCCG TCTTACAGAA CCCTCTCCCA AGCCAGCTGG TATTTTCTGG GCTAAAGACT 
GTACAGCCAG TTCCTCCTTT TTATAGAAGT TTACTTACGC CAGGGGAAAT GGTGAGTCCT 
CCAACCTCTC TCCCAACCAG TCCCATCATT CCAACCAGTG GCACCATAGA GCAGCACCCT 
CCTCCACCCT CTGAGCCAAT AGTGCCAGCA GTGATGATGG GTACTCACGA GCCCGGTGCT 
GACCTGGCAC CTAAGAAAAA GCCTAGGAAG TCAAGCATGC CTGTGAAAAT TGAGAAGGAA 
ATTATCGATA CTGCTGACGA GTTCGATGAT GAGGACGATG ATCCTAATGA TGGTGGGACC 
GTGGTCAACG ACATGAGTCA CGGCAATCAT TGTCATTCCC AAGACGAGAT GAGCCCAGGC 
ATGTCGGTGA AGGACTTCTC TAAGCATAAC AGGACCCGGT GCATTTCAAG AACTGAAATA 
AGAAGGGCTG ACAGTATGAC ATCTGAGGAC CAGGAGCCTG AGCGGGACTA TGAGAATGAG 
TCCGAGTCTT CAGAGCCTAA ACTCGGTGAG GAGTCCATGG TAGAAGATGA GCACATTCAC 
AGTGAGGTGA GCGACAAAGT CCTGATGAAC AGTGAACGAG CCGATGAGAA CCACAGTGAG 
CCCTCGCACC AGGATGTCAT CAAGGTCAAG GAGGAATTTA CAGACCCCAC TTACGACATG 
TTTTACATGA GCCAGTACGG ACTATACAAT GGCGGGGGGG CCAGCATGGC TGCCCTGCAT 
GATAGTTTTA CATCGTCTCT GAATTATGGA AGCCCTCAAA AATTCTCCCC TGAAGGTGAC 
CTGTGTTCTA GTCCAGACCC CAAAATCTGT TACGTGTGCA AGAAGAGCTT CAAAAGCTCC 
TACAGCGTGA AGCTTCACTA CAGGAACGTT CACTTAAAAG AAATGCATGT CTGCACAGTG 
GCTGGCTGCA ATGCTGCCTT CCCCTCTCGT CGAAGCAGAG ACAGACACAG TGCCAACATA 
AACCTGCATC GTAAACTGTT GACCAAAGAA CTCGATGACA TGGGCCTGGA CTCATCGCAG 
CCTTCCCTTA GCAAGGACCT CCGGGATGAA TTTTTGATGA AGATCTATGG TACCCAGCAC 
CCTCTGGGGC TCGATGTCAG GGAAGATGCC TCCTCTCCCG CAGGGACGGA AGACTCCCAC 
CTGAATGGGT ATGGGAGGGG CATGGCAGAG GACTACATGG TCCTTGACCT GAGCACCACC 
TCCAGCCTCC AGTCCAGCAG CAGTATCCAT TCCTCCAGAG AATCCGATGC AGGCAGCGAC 
GAGGGGATTC TTCTTGACGA CATTGATGGG GCGAGTGACA GTGGGGAGTC CGCTCACAAG 
GCTGAGGCCC CCACCCTCCC CAGCAGCCTT GGGGCCGAAG TTTCAGGATC TCTGATGTTC 
AGCAGTTTGT CTGGGAGCAA TGGTGGGATC ATGTGCAACA TTTGCCACAA AATGTACAGC 
AACAAGGGGA CCCTGAGGGT CCACTACAAA ACTGTGCATT TGCGAGAGAT GCACAAGTGC 
AAAGTCCCCG GCTGTAACAT GATGTTCTCC TCTGTGCGAA GCCGAAACAG GCACAGTCAG 
AACCCTAATC TCCACAAAAA CATTCCCTTC ACTTCAATAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448747.1
CDS	210..3614
Translation	MMDCRAWLAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEIDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	XM_017593258.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593258.1

ORF Insert Sequence:

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGTTCCACTG 
GCATGTGCTG GCAGAGTGCT GGGCGCAGAC TTCTGCCCAA ACCTGGAGGA GATAGACCAG 
AGGCTGGAAG TCCAGGCCAT TCGCTGCACA TTGGTGAACT GCACGTGTGA GTGTTTCCAG 
CCTGGCAAGA TTAACCTGCG GACCTGTGAT CAGTGTAAAC ATGGCTGGGT GGCCCATGCC 
TTGGATAAAC TCAGCACACA GCACCTATAC CACCCCACTC AAGTGGAGAT TGTGCAGTCC 
AACGTAGTGT TTGACATCAG CAGCTTGATG CTCTACGGGA CGCAGGCAGT GCCTGTGCGG 
CTAAAGATAC TGCTAGACCG GCTCTTCAGT GTCCTGAAGC AAGAGGAGGT GCTGCACATT 
CTCCACGGCC TGGGCTGGAC CCTGCGGGAC TATGTCCGAG GATACATCCT TCAGGACGCT 
GCTGGCAAAG TGCTGGACCG CTGGGCCATC ATGTCTCGGG AAGAGGAAAT CATCACCCTT 
CAGCAGTTTC TGCGGTTTGG GGAAACCAAA TCCATCGTGG AGCTTATGGC CATTCAGGAG 
AAAGAAGGGC AAGCTGTGGC CGTGCCGTCT TCAAAGACAG ACTCGGATAT AAGGACTTTC 
ATCGAGAGCA ATAATCGCAC CAGGAGCCCT AGCCTCCTCG CCCACTTAGA GAACAGCAAT 
CCTTCTAGCA TTCATCATTT TGAAAACATA CCGAACAGCC TTGCATTTCT GCTTCCGTTC 
CAGTATATAA ACCCTGTCTC TGCCCCACTG CTAGGGTTGC CACCCAACGG GCTGCTTTTA 
GAGCAGCCCG GACTGAGACT GAGGGAACCG AGCATTTCAA CCCAGAATGA GTACAACGAG 
AGCAGTGAGT CTGAAGTATC GCCTACACCA TATAAGAGTG ACCAGACGCC CAATAGAAAT 
GCCCTGACTA GCATCACTAA TGTGGAACCC AAAACTGAGC CAGCGTGTGT CTCCCCCATC 
CAGAATTCTG CCCCAGTCAG TGATCTAAGC AAACCTGAAC ACCCAAAAAG TTCATTCCGG 
ATTCACCGTA TGAGAAGGAT GGGGTCAGCC TCGAGGAAAG GAAGGGTTTT CTGTAATGCC 
TGTGGGAAGA CATTCTATGA CAAAGGCACT CTCAAAATCC ACTACAATGC TGTTCACCTG 
AAGATTAAAC ATCGATGTAC CATTGAAGGC TGCAACATGG TCTTTAGTTC CCTTCGAAGC 
CGGAATCGTC ACAGTGCAAA CCCCAACCCC CGCCTTCATA TGCCTATGCT AAGGAATAAC 
CGAGACAAAG ATTTAATTCG GGCTACATCA GGAGCCGCCA CTCCAGTCAT AGCAAGTACA 
AAATCAAATC TCACACTCAC GAGTCCTGGC CGGCCCCCGA TGGGTTTTAC CACTCCTCCA 
CTAGACCCCG TCTTACAGAA CCCTCTCCCA AGCCAGCTGG TATTTTCTGG GCTAAAGACT 
GTACAGCCAG TTCCTCCTTT TTATAGAAGT TTACTTACGC CAGGGGAAAT GGTGAGTCCT 
CCAACCTCTC TCCCAACCAG TCCCATCATT CCAACCAGTG GCACCATAGA GCAGCACCCT 
CCTCCACCCT CTGAGCCAAT AGTGCCAGCA GTGATGATGG GTACTCACGA GCCCGGTGCT 
GACCTGGCAC CTAAGAAAAA GCCTAGGAAG TCAAGCATGC CTGTGAAAAT TGAGAAGGAA 
ATTATCGATA CTGCTGACGA GTTCGATGAT GAGGACGATG ATCCTAATGA TGGTGGGACC 
GTGGTCAACG ACATGAGTCA CGGCAATCAT TGTCATTCCC AAGACGAGAT GAGCCCAGGC 
ATGTCGGTGA AGGACTTCTC TAAGCATAAC AGGACCCGGT GCATTTCAAG AACTGAAATA 
AGAAGGGCTG ACAGTATGAC ATCTGAGGAC CAGGAGCCTG AGCGGGACTA TGAGAATGAG 
TCCGAGTCTT CAGAGCCTAA ACTCGGTGAG GAGTCCATGG TAGAAGATGA GCACATTCAC 
AGTGAGGTGA GCGACAAAGT CCTGATGAAC AGTGAACGAG CCGATGAGAA CCACAGTGAG 
CCCTCGCACC AGGATGTCAT CAAGGTCAAG GAGGAATTTA CAGACCCCAC TTACGACATG 
TTTTACATGA GCCAGTACGG ACTATACAAT GGCGGGGGGG CCAGCATGGC TGCCCTGCAT 
GATAGTTTTA CATCGTCTCT GAATTATGGA AGCCCTCAAA AATTCTCCCC TGAAGGTGAC 
CTGTGTTCTA GTCCAGACCC CAAAATCTGT TACGTGTGCA AGAAGAGCTT CAAAAGCTCC 
TACAGCGTGA AGCTTCACTA CAGGAACGTT CACTTAAAAG AAATGCATGT CTGCACAGTG 
GCTGGCTGCA ATGCTGCCTT CCCCTCTCGT CGAAGCAGAG ACAGACACAG TGCCAACATA 
AACCTGCATC GTAAACTGTT GACCAAAGAA CTCGATGACA TGGGCCTGGA CTCATCGCAG 
CCTTCCCTTA GCAAGGACCT CCGGGATGAA TTTTTGATGA AGATCTATGG TACCCAGCAC 
CCTCTGGGGC TCGATGTCAG GGAAGATGCC TCCTCTCCCG CAGGGACGGA AGACTCCCAC 
CTGAATGGGT ATGGGAGGGG CATGGCAGAG GACTACATGG TCCTTGACCT GAGCACCACC 
TCCAGCCTCC AGTCCAGCAG CAGTATCCAT TCCTCCAGAG AATCCGATGC AGGCAGCGAC 
GAGGGGATTC TTCTTGACGA CATTGATGGG GCGAGTGACA GTGGGGAGTC CGCTCACAAG 
GCTGAGGCCC CCACCCTCCC CAGCAGCCTT GGGGCCGAAG TTTCAGGATC TCTGATGTTC 
AGCAGTTTGT CTGGGAGCAA TGGTGGGATC ATGTGCAACA TTTGCCACAA AATGTACAGC 
AACAAGGGGA CCCTGAGGGT CCACTACAAA ACTGTGCATT TGCGAGAGAT GCACAAGTGC 
AAAGTCCCCG GCTGTAACAT GATGTTCTCC TCTGTGCGAA GCCGAAACAG GCACAGTCAG 
AACCCTAATC TCCACAAAAA CATTCCCTTC ACTTCAATAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( XM_017593259.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448748.1 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X4, mRNA.

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CloneID	ORa61153
Clone ID Related Accession (Same CDS sequence)	XM_017593259.1
Accession Version	XM_017593259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACATT TTGTGCCCTC CCCACCTCCC AATTGCCTCA GTTACAAGTC GGAGGGCAGG 
CTCGGTGATC AAGACTGGCA AGCACATTTT AAGGTCCCAT GCTGTGGCGT TGACCCATCT 
CAACTTGAGT CAGAAGAGGC AGAAGTGGAT GTGAGAGAAA GAGACACACA GAGAGACAGA 
GAGCCAAAGA GGGCAAGAGA CTTGACTTTA AGAGACTCCT GTACTGACAA CTCCATGCAG 
TTCGGAACCA GAACGACTGC GGCTGAACCA GGGTTCATGG GGACATGGCA AAACGCTGAT 
ACTAACCTCT TATTCAGAAT GTCCCAACAG GTTCCACTGG CATGTGCTGG CAGAGTGCTG 
GGCGCAGACT TCTGCCCAAA CCTGGAGGAG ATAGACCAGA GGCTGGAAGT CCAGGCCATT 
CGCTGCACAT TGGTGAACTG CACGTGTGAG TGTTTCCAGC CTGGCAAGAT TAACCTGCGG 
ACCTGTGATC AGTGTAAACA TGGCTGGGTG GCCCATGCCT TGGATAAACT CAGCACACAG 
CACCTATACC ACCCCACTCA AGTGGAGATT GTGCAGTCCA ACGTAGTGTT TGACATCAGC 
AGCTTGATGC TCTACGGGAC GCAGGCAGTG CCTGTGCGGC TAAAGATACT GCTAGACCGG 
CTCTTCAGTG TCCTGAAGCA AGAGGAGGTG CTGCACATTC TCCACGGCCT GGGCTGGACC 
CTGCGGGACT ATGTCCGAGG ATACATCCTT CAGGACGCTG CTGGCAAAGT GCTGGACCGC 
TGGGCCATCA TGTCTCGGGA AGAGGAAATC ATCACCCTTC AGCAGTTTCT GCGGTTTGGG 
GAAACCAAAT CCATCGTGGA GCTTATGGCC ATTCAGGAGA AAGAAGGGCA AGCTGTGGCC 
GTGCCGTCTT CAAAGACAGA CTCGGATATA AGGACTTTCA TCGAGAGCAA TAATCGCACC 
AGGAGCCCTA GCCTCCTCGC CCACTTAGAG AACAGCAATC CTTCTAGCAT TCATCATTTT 
GAAAACATAC CGAACAGCCT TGCATTTCTG CTTCCGTTCC AGTATATAAA CCCTGTCTCT 
GCCCCACTGC TAGGGTTGCC ACCCAACGGG CTGCTTTTAG AGCAGCCCGG ACTGAGACTG 
AGGGAACCGA GCATTTCAAC CCAGAATGAG TACAACGAGA GCAGTGAGTC TGAAGTATCG 
CCTACACCAT ATAAGAGTGA CCAGACGCCC AATAGAAATG CCCTGACTAG CATCACTAAT 
GTGGAACCCA AAACTGAGCC AGCGTGTGTC TCCCCCATCC AGAATTCTGC CCCAGTCAGT 
GATCTAAGCA AACCTGAACA CCCAAAAAGT TCATTCCGGA TTCACCGTAT GAGAAGGATG 
GGGTCAGCCT CGAGGAAAGG AAGGGTTTTC TGTAATGCCT GTGGGAAGAC ATTCTATGAC 
AAAGGCACTC TCAAAATCCA CTACAATGCT GTTCACCTGA AGATTAAACA TCGATGTACC 
ATTGAAGGCT GCAACATGGT CTTTAGTTCC CTTCGAAGCC GGAATCGTCA CAGTGCAAAC 
CCCAACCCCC GCCTTCATAT GCCTATGCTA AGGAATAACC GAGACAAAGA TTTAATTCGG 
GCTACATCAG GAGCCGCCAC TCCAGTCATA GCAAGTACAA AATCAAATCT CACACTCACG 
AGTCCTGGCC GGCCCCCGAT GGGTTTTACC ACTCCTCCAC TAGACCCCGT CTTACAGAAC 
CCTCTCCCAA GCCAGCTGGT ATTTTCTGGG CTAAAGACTG TACAGCCAGT TCCTCCTTTT 
TATAGAAGTT TACTTACGCC AGGGGAAATG GTGAGTCCTC CAACCTCTCT CCCAACCAGT 
CCCATCATTC CAACCAGTGG CACCATAGAG CAGCACCCTC CTCCACCCTC TGAGCCAATA 
GTGCCAGCAG TGATGATGGG TACTCACGAG CCCGGTGCTG ACCTGGCACC TAAGAAAAAG 
CCTAGGAAGT CAAGCATGCC TGTGAAAATT GAGAAGGAAA TTATCGATAC TGCTGACGAG 
TTCGATGATG AGGACGATGA TCCTAATGAT GGTGGGACCG TGGTCAACGA CATGAGTCAC 
GGCAATCATT GTCATTCCCA AGACGAGATG AGCCCAGGCA TGTCGGTGAA GGACTTCTCT 
AAGCATAACA GGACCCGGTG CATTTCAAGA ACTGAAATAA GAAGGGCTGA CAGTATGACA 
TCTGAGGACC AGGAGCCTGA GCGGGACTAT GAGAATGAGT CCGAGTCTTC AGAGCCTAAA 
CTCGGTGAGG AGTCCATGGT AGAAGATGAG CACATTCACA GTGAGGTGAG CGACAAAGTC 
CTGATGAACA GTGAACGAGC CGATGAGAAC CACAGTGAGC CCTCGCACCA GGATGTCATC 
AAGGTCAAGG AGGAATTTAC AGACCCCACT TACGACATGT TTTACATGAG CCAGTACGGA 
CTATACAATG GCGGGGGGGC CAGCATGGCT GCCCTGCATG ATAGTTTTAC ATCGTCTCTG 
AATTATGGAA GCCCTCAAAA ATTCTCCCCT GAAGGTGACC TGTGTTCTAG TCCAGACCCC 
AAAATCTGTT ACGTGTGCAA GAAGAGCTTC AAAAGCTCCT ACAGCGTGAA GCTTCACTAC 
AGGAACGTTC ACTTAAAAGA AATGCATGTC TGCACAGTGG CTGGCTGCAA TGCTGCCTTC 
CCCTCTCGTC GAAGCAGAGA CAGACACAGT GCCAACATAA ACCTGCATCG TAAACTGTTG 
ACCAAAGAAC TCGATGACAT GGGCCTGGAC TCATCGCAGC CTTCCCTTAG CAAGGACCTC 
CGGGATGAAT TTTTGATGAA GATCTATGGT ACCCAGCACC CTCTGGGGCT CGATGTCAGG 
GAAGATGCCT CCTCTCCCGC AGGGACGGAA GACTCCCACC TGAATGGGTA TGGGAGGGGC 
ATGGCAGAGG ACTACATGGT CCTTGACCTG AGCACCACCT CCAGCCTCCA GTCCAGCAGC 
AGTATCCATT CCTCCAGAGA ATCCGATGCA GGCAGCGACG AGGGGATTCT TCTTGACGAC 
ATTGATGGGG CGAGTGACAG TGGGGAGTCC GCTCACAAGG CTGAGGCCCC CACCCTCCCC 
AGCAGCCTTG GGGCCGAAGT TTCAGGATCT CTGATGTTCA GCAGTTTGTC TGGGAGCAAT 
GGTGGGATCA TGTGCAACAT TTGCCACAAA ATGTACAGCA ACAAGGGGAC CCTGAGGGTC 
CACTACAAAA CTGTGCATTT GCGAGAGATG CACAAGTGCA AAGTCCCCGG CTGTAACATG 
ATGTTCTCCT CTGTGCGAAG CCGAAACAGG CACAGTCAGA ACCCTAATCT CCACAAAAAC 
ATTCCCTTCA CTTCAATAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448748.1
CDS	162..3545
Translation	MAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEIDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	XM_017593259.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593259.1

ORF Insert Sequence:

ATGGCACATT TTGTGCCCTC CCCACCTCCC AATTGCCTCA GTTACAAGTC GGAGGGCAGG 
CTCGGTGATC AAGACTGGCA AGCACATTTT AAGGTCCCAT GCTGTGGCGT TGACCCATCT 
CAACTTGAGT CAGAAGAGGC AGAAGTGGAT GTGAGAGAAA GAGACACACA GAGAGACAGA 
GAGCCAAAGA GGGCAAGAGA CTTGACTTTA AGAGACTCCT GTACTGACAA CTCCATGCAG 
TTCGGAACCA GAACGACTGC GGCTGAACCA GGGTTCATGG GGACATGGCA AAACGCTGAT 
ACTAACCTCT TATTCAGAAT GTCCCAACAG GTTCCACTGG CATGTGCTGG CAGAGTGCTG 
GGCGCAGACT TCTGCCCAAA CCTGGAGGAG ATAGACCAGA GGCTGGAAGT CCAGGCCATT 
CGCTGCACAT TGGTGAACTG CACGTGTGAG TGTTTCCAGC CTGGCAAGAT TAACCTGCGG 
ACCTGTGATC AGTGTAAACA TGGCTGGGTG GCCCATGCCT TGGATAAACT CAGCACACAG 
CACCTATACC ACCCCACTCA AGTGGAGATT GTGCAGTCCA ACGTAGTGTT TGACATCAGC 
AGCTTGATGC TCTACGGGAC GCAGGCAGTG CCTGTGCGGC TAAAGATACT GCTAGACCGG 
CTCTTCAGTG TCCTGAAGCA AGAGGAGGTG CTGCACATTC TCCACGGCCT GGGCTGGACC 
CTGCGGGACT ATGTCCGAGG ATACATCCTT CAGGACGCTG CTGGCAAAGT GCTGGACCGC 
TGGGCCATCA TGTCTCGGGA AGAGGAAATC ATCACCCTTC AGCAGTTTCT GCGGTTTGGG 
GAAACCAAAT CCATCGTGGA GCTTATGGCC ATTCAGGAGA AAGAAGGGCA AGCTGTGGCC 
GTGCCGTCTT CAAAGACAGA CTCGGATATA AGGACTTTCA TCGAGAGCAA TAATCGCACC 
AGGAGCCCTA GCCTCCTCGC CCACTTAGAG AACAGCAATC CTTCTAGCAT TCATCATTTT 
GAAAACATAC CGAACAGCCT TGCATTTCTG CTTCCGTTCC AGTATATAAA CCCTGTCTCT 
GCCCCACTGC TAGGGTTGCC ACCCAACGGG CTGCTTTTAG AGCAGCCCGG ACTGAGACTG 
AGGGAACCGA GCATTTCAAC CCAGAATGAG TACAACGAGA GCAGTGAGTC TGAAGTATCG 
CCTACACCAT ATAAGAGTGA CCAGACGCCC AATAGAAATG CCCTGACTAG CATCACTAAT 
GTGGAACCCA AAACTGAGCC AGCGTGTGTC TCCCCCATCC AGAATTCTGC CCCAGTCAGT 
GATCTAAGCA AACCTGAACA CCCAAAAAGT TCATTCCGGA TTCACCGTAT GAGAAGGATG 
GGGTCAGCCT CGAGGAAAGG AAGGGTTTTC TGTAATGCCT GTGGGAAGAC ATTCTATGAC 
AAAGGCACTC TCAAAATCCA CTACAATGCT GTTCACCTGA AGATTAAACA TCGATGTACC 
ATTGAAGGCT GCAACATGGT CTTTAGTTCC CTTCGAAGCC GGAATCGTCA CAGTGCAAAC 
CCCAACCCCC GCCTTCATAT GCCTATGCTA AGGAATAACC GAGACAAAGA TTTAATTCGG 
GCTACATCAG GAGCCGCCAC TCCAGTCATA GCAAGTACAA AATCAAATCT CACACTCACG 
AGTCCTGGCC GGCCCCCGAT GGGTTTTACC ACTCCTCCAC TAGACCCCGT CTTACAGAAC 
CCTCTCCCAA GCCAGCTGGT ATTTTCTGGG CTAAAGACTG TACAGCCAGT TCCTCCTTTT 
TATAGAAGTT TACTTACGCC AGGGGAAATG GTGAGTCCTC CAACCTCTCT CCCAACCAGT 
CCCATCATTC CAACCAGTGG CACCATAGAG CAGCACCCTC CTCCACCCTC TGAGCCAATA 
GTGCCAGCAG TGATGATGGG TACTCACGAG CCCGGTGCTG ACCTGGCACC TAAGAAAAAG 
CCTAGGAAGT CAAGCATGCC TGTGAAAATT GAGAAGGAAA TTATCGATAC TGCTGACGAG 
TTCGATGATG AGGACGATGA TCCTAATGAT GGTGGGACCG TGGTCAACGA CATGAGTCAC 
GGCAATCATT GTCATTCCCA AGACGAGATG AGCCCAGGCA TGTCGGTGAA GGACTTCTCT 
AAGCATAACA GGACCCGGTG CATTTCAAGA ACTGAAATAA GAAGGGCTGA CAGTATGACA 
TCTGAGGACC AGGAGCCTGA GCGGGACTAT GAGAATGAGT CCGAGTCTTC AGAGCCTAAA 
CTCGGTGAGG AGTCCATGGT AGAAGATGAG CACATTCACA GTGAGGTGAG CGACAAAGTC 
CTGATGAACA GTGAACGAGC CGATGAGAAC CACAGTGAGC CCTCGCACCA GGATGTCATC 
AAGGTCAAGG AGGAATTTAC AGACCCCACT TACGACATGT TTTACATGAG CCAGTACGGA 
CTATACAATG GCGGGGGGGC CAGCATGGCT GCCCTGCATG ATAGTTTTAC ATCGTCTCTG 
AATTATGGAA GCCCTCAAAA ATTCTCCCCT GAAGGTGACC TGTGTTCTAG TCCAGACCCC 
AAAATCTGTT ACGTGTGCAA GAAGAGCTTC AAAAGCTCCT ACAGCGTGAA GCTTCACTAC 
AGGAACGTTC ACTTAAAAGA AATGCATGTC TGCACAGTGG CTGGCTGCAA TGCTGCCTTC 
CCCTCTCGTC GAAGCAGAGA CAGACACAGT GCCAACATAA ACCTGCATCG TAAACTGTTG 
ACCAAAGAAC TCGATGACAT GGGCCTGGAC TCATCGCAGC CTTCCCTTAG CAAGGACCTC 
CGGGATGAAT TTTTGATGAA GATCTATGGT ACCCAGCACC CTCTGGGGCT CGATGTCAGG 
GAAGATGCCT CCTCTCCCGC AGGGACGGAA GACTCCCACC TGAATGGGTA TGGGAGGGGC 
ATGGCAGAGG ACTACATGGT CCTTGACCTG AGCACCACCT CCAGCCTCCA GTCCAGCAGC 
AGTATCCATT CCTCCAGAGA ATCCGATGCA GGCAGCGACG AGGGGATTCT TCTTGACGAC 
ATTGATGGGG CGAGTGACAG TGGGGAGTCC GCTCACAAGG CTGAGGCCCC CACCCTCCCC 
AGCAGCCTTG GGGCCGAAGT TTCAGGATCT CTGATGTTCA GCAGTTTGTC TGGGAGCAAT 
GGTGGGATCA TGTGCAACAT TTGCCACAAA ATGTACAGCA ACAAGGGGAC CCTGAGGGTC 
CACTACAAAA CTGTGCATTT GCGAGAGATG CACAAGTGCA AAGTCCCCGG CTGTAACATG 
ATGTTCTCCT CTGTGCGAAG CCGAAACAGG CACAGTCAGA ACCCTAATCT CCACAAAAAC 
ATTCCCTTCA CTTCAATAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( XM_008763776.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761998.2 Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X5, mRNA.

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CloneID	ORa61154
Clone ID Related Accession (Same CDS sequence)	XM_008763776.2
Accession Version	XM_008763776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGCCATT 
CGCTGCACAT TGGTGAACTG CACGTGTGAG TGTTTCCAGC CTGGCAAGAT TAACCTGCGG 
ACCTGTGATC AGTGTAAACA TGGCTGGGTG GCCCATGCCT TGGATAAACT CAGCACACAG 
CACCTATACC ACCCCACTCA AGTGGAGATT GTGCAGTCCA ACGTAGTGTT TGACATCAGC 
AGCTTGATGC TCTACGGGAC GCAGGCAGTG CCTGTGCGGC TAAAGATACT GCTAGACCGG 
CTCTTCAGTG TCCTGAAGCA AGAGGAGGTG CTGCACATTC TCCACGGCCT GGGCTGGACC 
CTGCGGGACT ATGTCCGAGG ATACATCCTT CAGGACGCTG CTGGCAAAGT GCTGGACCGC 
TGGGCCATCA TGTCTCGGGA AGAGGAAATC ATCACCCTTC AGCAGTTTCT GCGGTTTGGG 
GAAACCAAAT CCATCGTGGA GCTTATGGCC ATTCAGGAGA AAGAAGGGCA AGCTGTGGCC 
GTGCCGTCTT CAAAGACAGA CTCGGATATA AGGACTTTCA TCGAGAGCAA TAATCGCACC 
AGGAGCCCTA GCCTCCTCGC CCACTTAGAG AACAGCAATC CTTCTAGCAT TCATCATTTT 
GAAAACATAC CGAACAGCCT TGCATTTCTG CTTCCGTTCC AGTATATAAA CCCTGTCTCT 
GCCCCACTGC TAGGGTTGCC ACCCAACGGG CTGCTTTTAG AGCAGCCCGG ACTGAGACTG 
AGGGAACCGA GCATTTCAAC CCAGAATGAG TACAACGAGA GCAGTGAGTC TGAAGTATCG 
CCTACACCAT ATAAGAGTGA CCAGACGCCC AATAGAAATG CCCTGACTAG CATCACTAAT 
GTGGAACCCA AAACTGAGCC AGCGTGTGTC TCCCCCATCC AGAATTCTGC CCCAGTCAGT 
GATCTAAGCA AACCTGAACA CCCAAAAAGT TCATTCCGGA TTCACCGTAT GAGAAGGATG 
GGGTCAGCCT CGAGGAAAGG AAGGGTTTTC TGTAATGCCT GTGGGAAGAC ATTCTATGAC 
AAAGGCACTC TCAAAATCCA CTACAATGCT GTTCACCTGA AGATTAAACA TCGATGTACC 
ATTGAAGGCT GCAACATGGT CTTTAGTTCC CTTCGAAGCC GGAATCGTCA CAGTGCAAAC 
CCCAACCCCC GCCTTCATAT GCCTATGCTA AGGAATAACC GAGACAAAGA TTTAATTCGG 
GCTACATCAG GAGCCGCCAC TCCAGTCATA GCAAGTACAA AATCAAATCT CACACTCACG 
AGTCCTGGCC GGCCCCCGAT GGGTTTTACC ACTCCTCCAC TAGACCCCGT CTTACAGAAC 
CCTCTCCCAA GCCAGCTGGT ATTTTCTGGG CTAAAGACTG TACAGCCAGT TCCTCCTTTT 
TATAGAAGTT TACTTACGCC AGGGGAAATG GTGAGTCCTC CAACCTCTCT CCCAACCAGT 
CCCATCATTC CAACCAGTGG CACCATAGAG CAGCACCCTC CTCCACCCTC TGAGCCAATA 
GTGCCAGCAG TGATGATGGG TACTCACGAG CCCGGTGCTG ACCTGGCACC TAAGAAAAAG 
CCTAGGAAGT CAAGCATGCC TGTGAAAATT GAGAAGGAAA TTATCGATAC TGCTGACGAG 
TTCGATGATG AGGACGATGA TCCTAATGAT GGTGGGACCG TGGTCAACGA CATGAGTCAC 
GGCAATCATT GTCATTCCCA AGACGAGATG AGCCCAGGCA TGTCGGTGAA GGACTTCTCT 
AAGCATAACA GGACCCGGTG CATTTCAAGA ACTGAAATAA GAAGGGCTGA CAGTATGACA 
TCTGAGGACC AGGAGCCTGA GCGGGACTAT GAGAATGAGT CCGAGTCTTC AGAGCCTAAA 
CTCGGTGAGG AGTCCATGGT AGAAGATGAG CACATTCACA GTGAGGTGAG CGACAAAGTC 
CTGATGAACA GTGAACGAGC CGATGAGAAC CACAGTGAGC CCTCGCACCA GGATGTCATC 
AAGGTCAAGG AGGAATTTAC AGACCCCACT TACGACATGT TTTACATGAG CCAGTACGGA 
CTATACAATG GCGGGGGGGC CAGCATGGCT GCCCTGCATG ATAGTTTTAC ATCGTCTCTG 
AATTATGGAA GCCCTCAAAA ATTCTCCCCT GAAGGTGACC TGTGTTCTAG TCCAGACCCC 
AAAATCTGTT ACGTGTGCAA GAAGAGCTTC AAAAGCTCCT ACAGCGTGAA GCTTCACTAC 
AGGAACGTTC ACTTAAAAGA AATGCATGTC TGCACAGTGG CTGGCTGCAA TGCTGCCTTC 
CCCTCTCGTC GAAGCAGAGA CAGACACAGT GCCAACATAA ACCTGCATCG TAAACTGTTG 
ACCAAAGAAC TCGATGACAT GGGCCTGGAC TCATCGCAGC CTTCCCTTAG CAAGGACCTC 
CGGGATGAAT TTTTGATGAA GATCTATGGT ACCCAGCACC CTCTGGGGCT CGATGTCAGG 
GAAGATGCCT CCTCTCCCGC AGGGACGGAA GACTCCCACC TGAATGGGTA TGGGAGGGGC 
ATGGCAGAGG ACTACATGGT CCTTGACCTG AGCACCACCT CCAGCCTCCA GTCCAGCAGC 
AGTATCCATT CCTCCAGAGA ATCCGATGCA GGCAGCGACG AGGGGATTCT TCTTGACGAC 
ATTGATGGGG CGAGTGACAG TGGGGAGTCC GCTCACAAGG CTGAGGCCCC CACCCTCCCC 
AGCAGCCTTG GGGCCGAAGT TTCAGGATCT CTGATGTTCA GCAGTTTGTC TGGGAGCAAT 
GGTGGGATCA TGTGCAACAT TTGCCACAAA ATGTACAGCA ACAAGGGGAC CCTGAGGGTC 
CACTACAAAA CTGTGCATTT GCGAGAGATG CACAAGTGCA AAGTCCCCGG CTGTAACATG 
ATGTTCTCCT CTGTGCGAAG CCGAAACAGG CACAGTCAGA ACCCTAATCT CCACAAAAAC 
ATTCCCTTCA CTTCAATAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761998.2
CDS	2973..6296
Translation	MVLTNCSNTAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	XM_008763776.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763776.2

ORF Insert Sequence:

ATGGTGTTGA CCAATTGTTC CAATACGGCA CATTTTGTGC CCTCCCCACC TCCCAATTGC 
CTCAGTTACA AGTCGGAGGG CAGGCTCGGT GATCAAGACT GGCAAGCACA TTTTAAGGTC 
CCATGCTGTG GCGTTGACCC ATCTCAACTT GAGTCAGAAG AGGCAGAAGT GGATGTGAGA 
GAAAGAGACA CACAGAGAGA CAGAGAGCCA AAGAGGGCAA GAGACTTGAC TTTAAGAGAC 
TCCTGTACTG ACAACTCCAT GCAGTTCGGA ACCAGAACGA CTGCGGCTGA ACCAGGGTTC 
ATGGGGACAT GGCAAAACGC TGATACTAAC CTCTTATTCA GAATGTCCCA ACAGGCCATT 
CGCTGCACAT TGGTGAACTG CACGTGTGAG TGTTTCCAGC CTGGCAAGAT TAACCTGCGG 
ACCTGTGATC AGTGTAAACA TGGCTGGGTG GCCCATGCCT TGGATAAACT CAGCACACAG 
CACCTATACC ACCCCACTCA AGTGGAGATT GTGCAGTCCA ACGTAGTGTT TGACATCAGC 
AGCTTGATGC TCTACGGGAC GCAGGCAGTG CCTGTGCGGC TAAAGATACT GCTAGACCGG 
CTCTTCAGTG TCCTGAAGCA AGAGGAGGTG CTGCACATTC TCCACGGCCT GGGCTGGACC 
CTGCGGGACT ATGTCCGAGG ATACATCCTT CAGGACGCTG CTGGCAAAGT GCTGGACCGC 
TGGGCCATCA TGTCTCGGGA AGAGGAAATC ATCACCCTTC AGCAGTTTCT GCGGTTTGGG 
GAAACCAAAT CCATCGTGGA GCTTATGGCC ATTCAGGAGA AAGAAGGGCA AGCTGTGGCC 
GTGCCGTCTT CAAAGACAGA CTCGGATATA AGGACTTTCA TCGAGAGCAA TAATCGCACC 
AGGAGCCCTA GCCTCCTCGC CCACTTAGAG AACAGCAATC CTTCTAGCAT TCATCATTTT 
GAAAACATAC CGAACAGCCT TGCATTTCTG CTTCCGTTCC AGTATATAAA CCCTGTCTCT 
GCCCCACTGC TAGGGTTGCC ACCCAACGGG CTGCTTTTAG AGCAGCCCGG ACTGAGACTG 
AGGGAACCGA GCATTTCAAC CCAGAATGAG TACAACGAGA GCAGTGAGTC TGAAGTATCG 
CCTACACCAT ATAAGAGTGA CCAGACGCCC AATAGAAATG CCCTGACTAG CATCACTAAT 
GTGGAACCCA AAACTGAGCC AGCGTGTGTC TCCCCCATCC AGAATTCTGC CCCAGTCAGT 
GATCTAAGCA AACCTGAACA CCCAAAAAGT TCATTCCGGA TTCACCGTAT GAGAAGGATG 
GGGTCAGCCT CGAGGAAAGG AAGGGTTTTC TGTAATGCCT GTGGGAAGAC ATTCTATGAC 
AAAGGCACTC TCAAAATCCA CTACAATGCT GTTCACCTGA AGATTAAACA TCGATGTACC 
ATTGAAGGCT GCAACATGGT CTTTAGTTCC CTTCGAAGCC GGAATCGTCA CAGTGCAAAC 
CCCAACCCCC GCCTTCATAT GCCTATGCTA AGGAATAACC GAGACAAAGA TTTAATTCGG 
GCTACATCAG GAGCCGCCAC TCCAGTCATA GCAAGTACAA AATCAAATCT CACACTCACG 
AGTCCTGGCC GGCCCCCGAT GGGTTTTACC ACTCCTCCAC TAGACCCCGT CTTACAGAAC 
CCTCTCCCAA GCCAGCTGGT ATTTTCTGGG CTAAAGACTG TACAGCCAGT TCCTCCTTTT 
TATAGAAGTT TACTTACGCC AGGGGAAATG GTGAGTCCTC CAACCTCTCT CCCAACCAGT 
CCCATCATTC CAACCAGTGG CACCATAGAG CAGCACCCTC CTCCACCCTC TGAGCCAATA 
GTGCCAGCAG TGATGATGGG TACTCACGAG CCCGGTGCTG ACCTGGCACC TAAGAAAAAG 
CCTAGGAAGT CAAGCATGCC TGTGAAAATT GAGAAGGAAA TTATCGATAC TGCTGACGAG 
TTCGATGATG AGGACGATGA TCCTAATGAT GGTGGGACCG TGGTCAACGA CATGAGTCAC 
GGCAATCATT GTCATTCCCA AGACGAGATG AGCCCAGGCA TGTCGGTGAA GGACTTCTCT 
AAGCATAACA GGACCCGGTG CATTTCAAGA ACTGAAATAA GAAGGGCTGA CAGTATGACA 
TCTGAGGACC AGGAGCCTGA GCGGGACTAT GAGAATGAGT CCGAGTCTTC AGAGCCTAAA 
CTCGGTGAGG AGTCCATGGT AGAAGATGAG CACATTCACA GTGAGGTGAG CGACAAAGTC 
CTGATGAACA GTGAACGAGC CGATGAGAAC CACAGTGAGC CCTCGCACCA GGATGTCATC 
AAGGTCAAGG AGGAATTTAC AGACCCCACT TACGACATGT TTTACATGAG CCAGTACGGA 
CTATACAATG GCGGGGGGGC CAGCATGGCT GCCCTGCATG ATAGTTTTAC ATCGTCTCTG 
AATTATGGAA GCCCTCAAAA ATTCTCCCCT GAAGGTGACC TGTGTTCTAG TCCAGACCCC 
AAAATCTGTT ACGTGTGCAA GAAGAGCTTC AAAAGCTCCT ACAGCGTGAA GCTTCACTAC 
AGGAACGTTC ACTTAAAAGA AATGCATGTC TGCACAGTGG CTGGCTGCAA TGCTGCCTTC 
CCCTCTCGTC GAAGCAGAGA CAGACACAGT GCCAACATAA ACCTGCATCG TAAACTGTTG 
ACCAAAGAAC TCGATGACAT GGGCCTGGAC TCATCGCAGC CTTCCCTTAG CAAGGACCTC 
CGGGATGAAT TTTTGATGAA GATCTATGGT ACCCAGCACC CTCTGGGGCT CGATGTCAGG 
GAAGATGCCT CCTCTCCCGC AGGGACGGAA GACTCCCACC TGAATGGGTA TGGGAGGGGC 
ATGGCAGAGG ACTACATGGT CCTTGACCTG AGCACCACCT CCAGCCTCCA GTCCAGCAGC 
AGTATCCATT CCTCCAGAGA ATCCGATGCA GGCAGCGACG AGGGGATTCT TCTTGACGAC 
ATTGATGGGG CGAGTGACAG TGGGGAGTCC GCTCACAAGG CTGAGGCCCC CACCCTCCCC 
AGCAGCCTTG GGGCCGAAGT TTCAGGATCT CTGATGTTCA GCAGTTTGTC TGGGAGCAAT 
GGTGGGATCA TGTGCAACAT TTGCCACAAA ATGTACAGCA ACAAGGGGAC CCTGAGGGTC 
CACTACAAAA CTGTGCATTT GCGAGAGATG CACAAGTGCA AAGTCCCCGG CTGTAACATG 
ATGTTCTCCT CTGTGCGAAG CCGAAACAGG CACAGTCAGA ACCCTAATCT CCACAAAAAC 
ATTCCCTTCA CTTCAATAGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bnc2 ( NM_001106666.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100136.1 Rattus norvegicus basonuclin 2 (Bnc2), mRNA.

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CloneID	ORa04899
Clone ID Related Accession (Same CDS sequence)	NM_001106666.1
Accession Version	NM_001106666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein basonuclin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473978.2. On or before Oct 4, 2007 this sequence version replaced XM_001060986.1, XM_233123.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGCCATTCGC 
TGCACATTGG TGAACTGCAC GTGTGAGTGT TTCCAGCCTG GCAAGATTAA CCTGCGGACC 
TGTGATCAGT GTAAACATGG CTGGGTGGCC CATGCCTTGG ATAAACTCAG CACACAGCAC 
CTATACCACC CCACTCAAGT GGAGATTGTG CAGTCCAACG TAGTGTTTGA CATCAGCAGC 
TTGATGCTCT ACGGGACGCA GGCAGTGCCT GTGCGGCTAA AGATACTGCT AGACCGGCTC 
TTCAGTGTCC TGAAGCAAGA GGAGGTGCTG CACATTCTCC ACGGCCTGGG CTGGACCCTG 
CGGGACTATG TCCGAGGATA CATCCTTCAG GACGCTGCTG GCAAAGTGCT GGACCGCTGG 
GCCATCATGT CTCGGGAAGA GGAAATCATC ACCCTTCAGC AGTTTCTGCG GTTTGGGGAA 
ACCAAATCCA TCGTGGAGCT TATGGCCATT CAGGAGAAAG AAGGGCAAGC TGTGGCCGTG 
CCGTCTTCAA AGACAGACTC GGATATAAGG ACTTTCATCG AGAGCAATAA TCGCACCAGG 
AGCCCTAGCC TCCTCGCCCA CTTAGAGAAC AGCAATCCTT CTAGCATTCA TCATTTTGAA 
AACATACCGA ACAGCCTTGC ATTTCTGCTT CCGTTCCAGT ATATAAACCC TGTCTCTGCC 
CCACTGCTAG GGTTGCCACC CAACGGGCTG CTTTTAGAGC AGCCCGGACT GAGACTGAGG 
GAACCGAGCA TTTCAACCCA GAATGAGTAC AACGAGAGCA GTGAGTCTGA AGTATCGCCT 
ACACCATATA AGAGTGACCA GACGCCCAAT AGAAATGCCC TGACTAGCAT CACTAATGTG 
GAACCCAAAA CTGAGCCAGC GTGTGTCTCC CCCATCCAGA ATTCTGCCCC AGTCAGTGAT 
CTAAGCAAAC CTGAACACCC AAAAAGTTCA TTCCGGATTC ACCGTATGAG AAGGATGGGG 
TCAGCCTCGA GGAAAGGAAG GGTTTTCTGT AATGCCTGTG GGAAGACATT CTATGACAAA 
GGCACTCTCA AAATCCACTA CAATGCTGTT CACCTGAAGA TTAAACATCG ATGTACCATT 
GAAGGCTGCA ACATGGTCTT TAGTTCCCTT CGAAGCCGGA ATCGTCACAG TGCAAACCCC 
AACCCCCGCC TTCATATGCC TATGCTAAGG AATAACCGAG ACAAAGATTT AATTCGGGCT 
ACATCAGGAG CCGCCACTCC AGTCATAGCA AGTACAAAAT CAAATCTCAC ACTCACGAGT 
CCTGGCCGGC CCCCGATGGG TTTTACCACT CCTCCACTAG ACCCCGTCTT ACAGAACCCT 
CTCCCAAGCC AGCTGGTATT TTCTGGGCTA AAGACTGTAC AGCCAGTTCC TCCTTTTTAT 
AGAAGTTTAC TTACGCCAGG GGAAATGGTG AGTCCTCCAA CCTCTCTCCC AACCAGTCCC 
ATCATTCCAA CCAGTGGCAC CATAGAGCAG CACCCTCCTC CACCCTCTGA GCCAATAGTG 
CCAGCAGTGA TGATGGGTAC TCACGAGCCC GGTGCTGACC TGGCACCTAA GAAAAAGCCT 
AGGAAGTCAA GCATGCCTGT GAAAATTGAG AAGGAAATTA TCGATACTGC TGACGAGTTC 
GATGATGAGG ACGATGATCC TAATGATGGT GGGACCGTGG TCAACGACAT GAGTCACGGC 
AATCATTGTC ATTCCCAAGA CGAGATGAGC CCAGGCATGT CGGTGAAGGA CTTCTCTAAG 
CATAACAGGA CCCGGTGCAT TTCAAGAACT GAAATAAGAA GGGCTGACAG TATGACATCT 
GAGGACCAGG AGCCTGAGCG GGACTATGAG AATGAGTCCG AGTCTTCAGA GCCTAAACTC 
GGTGAGGAGT CCATGGTAGA AGATGAGCAC ATTCACAGTG AGGTGAGCGA CAAAGTCCTG 
ATGAACAGTG AACGAGCCGA TGAGAACCAC AGTGAGCCCT CGCACCAGGA TGTCATCAAG 
GTCAAGGAGG AATTTACAGA CCCCACTTAC GACATGTTTT ACATGAGCCA GTACGGACTA 
TACAATGGCG GGGGGGCCAG CATGGCTGCC CTGCATGATA GTTTTACATC GTCTCTGAAT 
TATGGAAGCC CTCAAAAATT CTCCCCTGAA GGTGACCTGT GTTCTAGTCC AGACCCCAAA 
ATCTGTTACG TGTGCAAGAA GAGCTTCAAA AGCTCCTACA GCGTGAAGCT TCACTACAGG 
AACGTTCACT TAAAAGAAAT GCATGTCTGC ACAGTGGCTG GCTGCAATGC TGCCTTCCCC 
TCTCGTCGAA GCAGAGACAG ACACAGTGCC AACATAAACC TGCATCGTAA ACTGTTGACC 
AAAGAACTCG ATGACATGGG CCTGGACTCA TCGCAGCCTT CCCTTAGCAA GGACCTCCGG 
GATGAATTTT TGATGAAGAT CTATGGTACC CAGCACCCTC TGGGGCTCGA TGTCAGGGAA 
GATGCCTCCT CTCCCGCAGG GACGGAAGAC TCCCACCTGA ATGGGTATGG GAGGGGCATG 
GCAGAGGACT ACATGGTCCT TGACCTGAGC ACCACCTCCA GCCTCCAGTC CAGCAGCAGT 
ATCCATTCCT CCAGAGAATC CGATGCAGGC AGCGACGAGG GGATTCTTCT TGACGACATT 
GATGGGGCGA GTGACAGTGG GGAGTCCGCT CACAAGGCTG AGGCCCCCAC CCTCCCCAGC 
AGCCTTGGGG CCGAAGTTTC AGGATCTCTG ATGTTCAGCA GTTTGTCTGG GAGCAATGGT 
GGGATCATGT GCAACATTTG CCACAAAATG TACAGCAACA AGGGGACCCT GAGGGTCCAC 
TACAAAACTG TGCATTTGCG AGAGATGCAC AAGTGCAAAG TCCCCGGCTG TAACATGATG 
TTCTCCTCTG TGCGAAGCCG AAACAGGCAC AGTCAGAACC CTAATCTCCA CAAAAACATT 
CCCTTCACTT CAATAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100136.1
CDS	67..3387
Translation	MMDCRAWLAHFVPSPPPNCLSYKSEGRLGDQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKPEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPGADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHGNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMVEDEHIHSEVSDKVLMNSERADENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHDSFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLMKIYGTQHPLGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPTLPSSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID

Target ORF information:

RefSeq Version	NM_001106666.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basonuclin 2 (Bnc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106666.1

ORF Insert Sequence:

ATGATGGATT GCAGAGCCTG GCTGGCACAT TTTGTGCCCT CCCCACCTCC CAATTGCCTC 
AGTTACAAGT CGGAGGGCAG GCTCGGTGAT CAAGACTGGC AAGCACATTT TAAGGTCCCA 
TGCTGTGGCG TTGACCCATC TCAACTTGAG TCAGAAGAGG CAGAAGTGGA TGTGAGAGAA 
AGAGACACAC AGAGAGACAG AGAGCCAAAG AGGGCAAGAG ACTTGACTTT AAGAGACTCC 
TGTACTGACA ACTCCATGCA GTTCGGAACC AGAACGACTG CGGCTGAACC AGGGTTCATG 
GGGACATGGC AAAACGCTGA TACTAACCTC TTATTCAGAA TGTCCCAACA GGCCATTCGC 
TGCACATTGG TGAACTGCAC GTGTGAGTGT TTCCAGCCTG GCAAGATTAA CCTGCGGACC 
TGTGATCAGT GTAAACATGG CTGGGTGGCC CATGCCTTGG ATAAACTCAG CACACAGCAC 
CTATACCACC CCACTCAAGT GGAGATTGTG CAGTCCAACG TAGTGTTTGA CATCAGCAGC 
TTGATGCTCT ACGGGACGCA GGCAGTGCCT GTGCGGCTAA AGATACTGCT AGACCGGCTC 
TTCAGTGTCC TGAAGCAAGA GGAGGTGCTG CACATTCTCC ACGGCCTGGG CTGGACCCTG 
CGGGACTATG TCCGAGGATA CATCCTTCAG GACGCTGCTG GCAAAGTGCT GGACCGCTGG 
GCCATCATGT CTCGGGAAGA GGAAATCATC ACCCTTCAGC AGTTTCTGCG GTTTGGGGAA 
ACCAAATCCA TCGTGGAGCT TATGGCCATT CAGGAGAAAG AAGGGCAAGC TGTGGCCGTG 
CCGTCTTCAA AGACAGACTC GGATATAAGG ACTTTCATCG AGAGCAATAA TCGCACCAGG 
AGCCCTAGCC TCCTCGCCCA CTTAGAGAAC AGCAATCCTT CTAGCATTCA TCATTTTGAA 
AACATACCGA ACAGCCTTGC ATTTCTGCTT CCGTTCCAGT ATATAAACCC TGTCTCTGCC 
CCACTGCTAG GGTTGCCACC CAACGGGCTG CTTTTAGAGC AGCCCGGACT GAGACTGAGG 
GAACCGAGCA TTTCAACCCA GAATGAGTAC AACGAGAGCA GTGAGTCTGA AGTATCGCCT 
ACACCATATA AGAGTGACCA GACGCCCAAT AGAAATGCCC TGACTAGCAT CACTAATGTG 
GAACCCAAAA CTGAGCCAGC GTGTGTCTCC CCCATCCAGA ATTCTGCCCC AGTCAGTGAT 
CTAAGCAAAC CTGAACACCC AAAAAGTTCA TTCCGGATTC ACCGTATGAG AAGGATGGGG 
TCAGCCTCGA GGAAAGGAAG GGTTTTCTGT AATGCCTGTG GGAAGACATT CTATGACAAA 
GGCACTCTCA AAATCCACTA CAATGCTGTT CACCTGAAGA TTAAACATCG ATGTACCATT 
GAAGGCTGCA ACATGGTCTT TAGTTCCCTT CGAAGCCGGA ATCGTCACAG TGCAAACCCC 
AACCCCCGCC TTCATATGCC TATGCTAAGG AATAACCGAG ACAAAGATTT AATTCGGGCT 
ACATCAGGAG CCGCCACTCC AGTCATAGCA AGTACAAAAT CAAATCTCAC ACTCACGAGT 
CCTGGCCGGC CCCCGATGGG TTTTACCACT CCTCCACTAG ACCCCGTCTT ACAGAACCCT 
CTCCCAAGCC AGCTGGTATT TTCTGGGCTA AAGACTGTAC AGCCAGTTCC TCCTTTTTAT 
AGAAGTTTAC TTACGCCAGG GGAAATGGTG AGTCCTCCAA CCTCTCTCCC AACCAGTCCC 
ATCATTCCAA CCAGTGGCAC CATAGAGCAG CACCCTCCTC CACCCTCTGA GCCAATAGTG 
CCAGCAGTGA TGATGGGTAC TCACGAGCCC GGTGCTGACC TGGCACCTAA GAAAAAGCCT 
AGGAAGTCAA GCATGCCTGT GAAAATTGAG AAGGAAATTA TCGATACTGC TGACGAGTTC 
GATGATGAGG ACGATGATCC TAATGATGGT GGGACCGTGG TCAACGACAT GAGTCACGGC 
AATCATTGTC ATTCCCAAGA CGAGATGAGC CCAGGCATGT CGGTGAAGGA CTTCTCTAAG 
CATAACAGGA CCCGGTGCAT TTCAAGAACT GAAATAAGAA GGGCTGACAG TATGACATCT 
GAGGACCAGG AGCCTGAGCG GGACTATGAG AATGAGTCCG AGTCTTCAGA GCCTAAACTC 
GGTGAGGAGT CCATGGTAGA AGATGAGCAC ATTCACAGTG AGGTGAGCGA CAAAGTCCTG 
ATGAACAGTG AACGAGCCGA TGAGAACCAC AGTGAGCCCT CGCACCAGGA TGTCATCAAG 
GTCAAGGAGG AATTTACAGA CCCCACTTAC GACATGTTTT ACATGAGCCA GTACGGACTA 
TACAATGGCG GGGGGGCCAG CATGGCTGCC CTGCATGATA GTTTTACATC GTCTCTGAAT 
TATGGAAGCC CTCAAAAATT CTCCCCTGAA GGTGACCTGT GTTCTAGTCC AGACCCCAAA 
ATCTGTTACG TGTGCAAGAA GAGCTTCAAA AGCTCCTACA GCGTGAAGCT TCACTACAGG 
AACGTTCACT TAAAAGAAAT GCATGTCTGC ACAGTGGCTG GCTGCAATGC TGCCTTCCCC 
TCTCGTCGAA GCAGAGACAG ACACAGTGCC AACATAAACC TGCATCGTAA ACTGTTGACC 
AAAGAACTCG ATGACATGGG CCTGGACTCA TCGCAGCCTT CCCTTAGCAA GGACCTCCGG 
GATGAATTTT TGATGAAGAT CTATGGTACC CAGCACCCTC TGGGGCTCGA TGTCAGGGAA 
GATGCCTCCT CTCCCGCAGG GACGGAAGAC TCCCACCTGA ATGGGTATGG GAGGGGCATG 
GCAGAGGACT ACATGGTCCT TGACCTGAGC ACCACCTCCA GCCTCCAGTC CAGCAGCAGT 
ATCCATTCCT CCAGAGAATC CGATGCAGGC AGCGACGAGG GGATTCTTCT TGACGACATT 
GATGGGGCGA GTGACAGTGG GGAGTCCGCT CACAAGGCTG AGGCCCCCAC CCTCCCCAGC 
AGCCTTGGGG CCGAAGTTTC AGGATCTCTG ATGTTCAGCA GTTTGTCTGG GAGCAATGGT 
GGGATCATGT GCAACATTTG CCACAAAATG TACAGCAACA AGGGGACCCT GAGGGTCCAC 
TACAAAACTG TGCATTTGCG AGAGATGCAC AAGTGCAAAG TCCCCGGCTG TAACATGATG 
TTCTCCTCTG TGCGAAGCCG AAACAGGCAC AGTCAGAACC CTAATCTCCA CAAAAACATT 
CCCTTCACTT CAATAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins.
Proceedings of the National Academy of Sciences of the United States of America106(34)14432-7(2009 Aug)
Vanhoutteghem A,Maciejewski-Duval A,Bouche C,Delhomme B,Herv? F,Daubigney F,Soubigou G,Araki M,Araki K,Yamamura K,Djian P

Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes.
Genomics83(5)821-33(2004 May)
Romano RA,Li H,Tummala R,Maul R,Sinha S

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Bnc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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