Frem1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frem1
Entrez Gene ID	298185
Full Name	Fras1 related extracellular matrix 1
Synonyms	RGD1306981
General protein information	Preferred Names Fras1 related extracellular matrix 1 Names FRAS1-related extracellular matrix protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763770.2	XP_008761992.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_008763772.1	XP_008761994.1	FRAS1-related extracellular matrix protein 1 isoform X4
XM_006238349.3	XP_006238411.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_008763771.2	XP_008761993.1	FRAS1-related extracellular matrix protein 1 isoform X3
NM_001191757.1	NP_001178686.1	FRAS1-related extracellular matrix protein 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Expression of FRAS1-Related Extracellular Matrix 1 Is Decreased in Nitrofen-Induced Congenital Diaphragmatic Hernia.
Takahashi T, Friedmacher F, Puri P
European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie26(1)81-5(2016 Feb)

Deficiency of FRAS1-related extracellular matrix 1 (FREM1) causes congenital diaphragmatic hernia in humans and mice.
Beck TF, Veenma D, Shchelochkov OA, Yu Z, Kim BJ, Zaveri HP, van Bever Y, Choi S, Douben H, Bertin TK, Patel PI, Lee B, Tibboel D, de Klein A, Stockton DW, Justice MJ, Scott DA
Human molecular genetics22(5)1026-38(2013 Mar)

Novel frem1-related mouse phenotypes and evidence of genetic interactions with gata4 and slit3.
Beck TF, Shchelochkov OA, Yu Z, Kim BJ, Hern?ndez-Garc?a A, Zaveri HP, Bishop C, Overbeek PA, Stockton DW, Justice MJ, Scott DA
PloS one8(3)e58830(2013)

Heterozygous mutations of FREM1 are associated with an increased risk of isolated metopic craniosynostosis in humans and mice.
Vissers LE, Cox TC, Maga AM, Short KM, Wiradjaja F, Janssen IM, Jehee F, Bertola D, Liu J, Yagnik G, Sekiguchi K, Kiyozumi D, van Bokhoven H, Marcelis C, Cunningham ML, Anderson PJ, Boyadjiev SA, Passos-Bueno MR, Veltman JA, Smyth I, Buckley MF, Roscioli T
PLoS genetics7(9)e1002278(2011 Sep)

Manitoba-oculo-tricho-anal (MOTA) syndrome is caused by mutations in FREM1.
Slavotinek AM, Baranzini SE, Schanze D, Labelle-Dumais C, Short KM, Chao R, Yahyavi M, Bijlsma EK, Chu C, Musone S, Wheatley A, Kwok PY, Marles S, Fryns JP, Maga AM, Hassan MG, Gould DB, Madireddy L, Li C, Cox TC, Smyth I, Chudley AE, Zenker M
Journal of medical genetics48(6)375-82(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased expression of FREM1 in the nitrofen-induced congenital diaphragmatic hernia model may disturb the formation of the diaphragmatic mesenchyme, thus contributing to the development of diaphragmatic defects.
Title: Gene Expression of FRAS1-Related Extracellular Matrix 1 Is Decreased in Nitrofen-Induced Congenital Diaphragmatic Hernia.

The following Frem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa45502	XM_008763772.1 Latest version!	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa45501	XM_008763771.2 Latest version!	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa07566	NM_001191757.1 Latest version!	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa28279	XM_006238349.3 Latest version!	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa45500	XM_008763770.2 Latest version!	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frem1 ( XM_008763772.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761994.1 Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa45502
Clone ID Related Accession (Same CDS sequence)	XM_008763772.1
Accession Version	XM_008763772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAC AAGAGCCCAT GCTGAAGACT GCCTTGCCTC TCTTTGCCAG ATTTACCATC 
AGCAATGGAC TGCAGACCCA GCAGGGGGTG TTTGAGATCA CACTGCAGGG TGTGGACAGT 
GCCTTGCCTG TGCTGACCAG GAACCGAAGG CTGAGGCTTG TGGAAGGGGC CATGGGCCTG 
CTGTCCACGG ATCACCTTCA GCTGACTGAC CCTGACACAC CTCCAGAGAA CCTAACCTTC 
TTTCTGGTCC AGCTCCCGCG CCACGGGTAC CTCTACCTGA GAGGGAAAGT ACTTCAGCAC 
AACTTCACCC AGCAAGATGT AGACAGCAGG GTTGTGGCCT ACCAGCATGC AGGAGGCGGT 
GCCCAGGAGG ACTGCTTTAC TTTCCTGGCC ACAGACAGGA AGAACCAAGG CTTCGTTGTG 
GATGGGAAAG TTCAGAAAGA GCCCGTTCTC TTCATTATCC AGGTGGACCA GCTGGATAAG 
AAGGCGCCTC GGATCACACG CTTGCACTCC CCTTCTCAAG TGGGGCTCTT GAAAAATGGC 
TGCTATGGGA TTTACATCAC TTCCCATGTG CTAAAGGCGT CTGACCCTGA CACAGAGGAT 
GACCAGATCA TCTTTAAGAT TTTACGAGGC CCGTTGCATG GACGTCTGGA GAACACGACA 
ACAGGTGAAT TCATCCACGA GCAATTTAGC CAGAAGGACT TGAGCCGCAA GACCATCCTT 
TATATCATAA ACCCTTCTTT GCAAGTGAAT TCAGATATCC TGGAATTTCA GGCCATGGAC 
CCCACAGGGA ACACTGCCCC TCCCCAGAGA TTGGAACTGA AATGGTCATA TATTGAATGG 
TCCCAGACTG AATATGAGGT CTGTGAGAAT GTGGGCTTAC TGACCTTGGA AGTCACCAGA 
AGGGGATATT CCATGGACTC AGCCTATGTG GACATAGAGG TTAACCAAGT GTCAGCTGTA 
GTTGGAAAAG ATTTTACTGT CACTCCATCC AAACTGCTTC AATTTGACCC AGGAATGTCA 
ACTAAGATGT GGAATATAGC AATTACCTAT GACGGATTAG AGGAAGACGA TGAGGTCTTT 
GAAGTAATTC TGAACTCCCC TGTGAATGCG GTTCTTGGCA CACAGACAAA AGCAGCAGTG 
AAAATTTTGG ACTCAAAAGG AGGACGGTGC CATCCTTCAA ATTCCTTCAA CCAAAGCAAG 
CACAGCACGT GGGGGAAGGG ACGTTGGCAT CCACTGCCCT CAGGGTCATC CTCACTCACC 
ACTTCCGGTT CTCCTCATCT GGAAAGAAGG CCCCCTCCAT CCATCACCAA AGGAGATGCT 
CTTCGGGGTT TTGATCTCAC AGACCTCTCT CAAAGGAAGA TGACGACACA GGGGAATGGC 
AAATCAGTCC TCCCATCCTC TGTTTGTAGA AATGGAACAG ACACCATCTA CAATTATCAT 
GGTATAGTTT CCTTGAAACT GGAGGGGGAC AGATTCTCAG CTCACAAACG GAAGGCCAAA 
ATATCCGTCA TTCGTCAGCC ACAGAGGACA GTCAAGGTGG CAGAGCTGCC TCTAGCCGAT 
AAGGTAGAAC CCACAACCGA CTCGAACTTT CTCAGTCAGG GTCTACGGCC CTTGTTTCCA 
AAGAACTGTT CTGTGGAATT AAAGGGACTC TTTCATTTGG AAGAACACAC CCACAGATTG 
TATCAATGTG ATGGGATCGC CTGGAACGTC TGGAGCTCCC AAACCAAGGG GCTGGAGGAC 
AGATCCTGCC CAGTTGGATG GCTTCTTCAT TCCGACTACT GTCACGTCTT GGTCACAAGG 
CAGAAAGGCA CCTGGGCCAC AGCTACCCGA GCTTGCAGAG AACAGCACCA GGGCACCCTT 
GTGACCGTAT TCTCCAGGAG GCACATGCAG TGGCTGTGGG CTATCAGCGG AAGAAAGCCC 
TTCTGGATAG GCTTGAAGAA CCAAGTGCGA ACTGGCCACT GGGAGTGGAT TGGAGGTGAA 
CCTGTTGCTT TCACCAACTG GAGGAGAGGG CCCCCTCTGC ACCCGAAGCC TGGAAAGAAC 
TGTGTTTTGG TTCAAAGAAG AGGACAATGG CAAACAAAGA ACTGTAGCAA AGGCAAGGCT 
CACAATTTTG TGTGCTCAAG GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761994.1
CDS	89..2218
Translation	MGTQEPMLKTALPLFARFTISNGLQTQQGVFEITLQGVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL

Target ORF information:

RefSeq Version	XM_008763772.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763772.1

ORF Insert Sequence:

ATGGGGACAC AAGAGCCCAT GCTGAAGACT GCCTTGCCTC TCTTTGCCAG ATTTACCATC 
AGCAATGGAC TGCAGACCCA GCAGGGGGTG TTTGAGATCA CACTGCAGGG TGTGGACAGT 
GCCTTGCCTG TGCTGACCAG GAACCGAAGG CTGAGGCTTG TGGAAGGGGC CATGGGCCTG 
CTGTCCACGG ATCACCTTCA GCTGACTGAC CCTGACACAC CTCCAGAGAA CCTAACCTTC 
TTTCTGGTCC AGCTCCCGCG CCACGGGTAC CTCTACCTGA GAGGGAAAGT ACTTCAGCAC 
AACTTCACCC AGCAAGATGT AGACAGCAGG GTTGTGGCCT ACCAGCATGC AGGAGGCGGT 
GCCCAGGAGG ACTGCTTTAC TTTCCTGGCC ACAGACAGGA AGAACCAAGG CTTCGTTGTG 
GATGGGAAAG TTCAGAAAGA GCCCGTTCTC TTCATTATCC AGGTGGACCA GCTGGATAAG 
AAGGCGCCTC GGATCACACG CTTGCACTCC CCTTCTCAAG TGGGGCTCTT GAAAAATGGC 
TGCTATGGGA TTTACATCAC TTCCCATGTG CTAAAGGCGT CTGACCCTGA CACAGAGGAT 
GACCAGATCA TCTTTAAGAT TTTACGAGGC CCGTTGCATG GACGTCTGGA GAACACGACA 
ACAGGTGAAT TCATCCACGA GCAATTTAGC CAGAAGGACT TGAGCCGCAA GACCATCCTT 
TATATCATAA ACCCTTCTTT GCAAGTGAAT TCAGATATCC TGGAATTTCA GGCCATGGAC 
CCCACAGGGA ACACTGCCCC TCCCCAGAGA TTGGAACTGA AATGGTCATA TATTGAATGG 
TCCCAGACTG AATATGAGGT CTGTGAGAAT GTGGGCTTAC TGACCTTGGA AGTCACCAGA 
AGGGGATATT CCATGGACTC AGCCTATGTG GACATAGAGG TTAACCAAGT GTCAGCTGTA 
GTTGGAAAAG ATTTTACTGT CACTCCATCC AAACTGCTTC AATTTGACCC AGGAATGTCA 
ACTAAGATGT GGAATATAGC AATTACCTAT GACGGATTAG AGGAAGACGA TGAGGTCTTT 
GAAGTAATTC TGAACTCCCC TGTGAATGCG GTTCTTGGCA CACAGACAAA AGCAGCAGTG 
AAAATTTTGG ACTCAAAAGG AGGACGGTGC CATCCTTCAA ATTCCTTCAA CCAAAGCAAG 
CACAGCACGT GGGGGAAGGG ACGTTGGCAT CCACTGCCCT CAGGGTCATC CTCACTCACC 
ACTTCCGGTT CTCCTCATCT GGAAAGAAGG CCCCCTCCAT CCATCACCAA AGGAGATGCT 
CTTCGGGGTT TTGATCTCAC AGACCTCTCT CAAAGGAAGA TGACGACACA GGGGAATGGC 
AAATCAGTCC TCCCATCCTC TGTTTGTAGA AATGGAACAG ACACCATCTA CAATTATCAT 
GGTATAGTTT CCTTGAAACT GGAGGGGGAC AGATTCTCAG CTCACAAACG GAAGGCCAAA 
ATATCCGTCA TTCGTCAGCC ACAGAGGACA GTCAAGGTGG CAGAGCTGCC TCTAGCCGAT 
AAGGTAGAAC CCACAACCGA CTCGAACTTT CTCAGTCAGG GTCTACGGCC CTTGTTTCCA 
AAGAACTGTT CTGTGGAATT AAAGGGACTC TTTCATTTGG AAGAACACAC CCACAGATTG 
TATCAATGTG ATGGGATCGC CTGGAACGTC TGGAGCTCCC AAACCAAGGG GCTGGAGGAC 
AGATCCTGCC CAGTTGGATG GCTTCTTCAT TCCGACTACT GTCACGTCTT GGTCACAAGG 
CAGAAAGGCA CCTGGGCCAC AGCTACCCGA GCTTGCAGAG AACAGCACCA GGGCACCCTT 
GTGACCGTAT TCTCCAGGAG GCACATGCAG TGGCTGTGGG CTATCAGCGG AAGAAAGCCC 
TTCTGGATAG GCTTGAAGAA CCAAGTGCGA ACTGGCCACT GGGAGTGGAT TGGAGGTGAA 
CCTGTTGCTT TCACCAACTG GAGGAGAGGG CCCCCTCTGC ACCCGAAGCC TGGAAAGAAC 
TGTGTTTTGG TTCAAAGAAG AGGACAATGG CAAACAAAGA ACTGTAGCAA AGGCAAGGCT 
CACAATTTTG TGTGCTCAAG GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem1 ( XM_008763771.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761993.1 Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa45501
Clone ID Related Accession (Same CDS sequence)	XM_008763771.2
Accession Version	XM_008763771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATATTGA CCCCTCTGAC AACCTCCGTG 
CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGCTGG TGTTTAACAT CACTGAAGCC 
CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTTACC 
TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CGAACAACAG CCACCCTGAG 
AGGAGACATT ACAAGATGGA GCTGGAGGTG TACGACCTCT TCTTTGAGAG GAGCGCCCCC 
ATCACAGTTC ACATCTCCAT CAGAACAGCA GACACGAATG CACCTCGGGT GTCCTGGAAT 
ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA GTTTCAGATC 
GTGGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGTCT GCAGCATGGA 
CGGCTTACAG TAAGAGAGGG GAAAGGATTT CTCTTCACCG TGGCTGATCT TCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA 
TTCGATGGCC AACACAGCAG CCATCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC 
AGTCCCCCAT TCCTCATAAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG 
ATCCAAGGGT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGTTCCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGATTGATA 
GGATATCCTG TCTCTGGCTT CCTTCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCT TTTGAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ATGGTCCATA TAACTCCAGT AGACGACCAG 
CTTCCCAAGG AAGCTCCCGG CATATCCCGG CATTTGATTG TCAAGGAAAC GGAAGTGGCC 
TACATCACTA AAAAGCACCT ACATTTCCTA GACACGGAAT CGCATGGTGG GGAGCTTGTC 
TACACAATAA CACGCCCTCC ATGCTTCTCC TCCAGCCACA GGCACTTGGA TGCTGGGAAG 
CTGTTCATGG TGGACAGCAT ACCAAAGCCA ACCAAGAATC CCACAGCCCC AGGGCTGAAC 
TCATTTACTC AGCATGCTGT GAACCACATG AAAGTGGCCT ACATGCCACC CATGCAGGAT 
ATAGGTCCCA CACCTCGACA TGTGCAGTTC ACAGTGTCCG TCAGTAACCA GCATGGAGGT 
ACTTTGCATG GAATCTGCTT CAACATCACA GTGCTCCCAG TGGACAATCA GGTTCCTGAG 
GTATTCACCA ACACCCTGAG AGTGGCTGAG GGTGGCCAAT GCACCATCAG CACAGAGCAC 
ATTTTGGTTT CTGATGTGGA CACCCCACTG GACAGCATCT GCCTCTCTCT TCAGGAGATG 
CCCCTGCATG GCGGGGTAGA GCTGGATGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT 
TGGCGAGACC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGATCTGA GGTTCTTCAG 
GATGAGATAT TCTTGGAGGT CACAGACGGC ACAAATTCTG CAGGATTTGT TCTCTACATT 
GAGGTATTCC CTGTGAATGA TGAGCCACCT GTTCTAAAGG CTGACCTCAT TCCCATGATG 
CACTGCTCAG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTTCGC TACTGATGCA 
GACAGCAATG ACTCAGAACT GCTATTTCTG ATTACCCGAG AACCTCGGCA TGGAGTAGTG 
AGAAAAGCTG GACTCCGTGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGAGCTGTG 
GCATACAAAC ATACAGGTGG CGAGATTGGC CTCGAATCTT GTTTTGACAC TGTTGTATTG 
GTGGTTTCGG ATGGTGAAGC TGACCCTTTG GTGAATGGCT GTTGCTACAA TGGACCCGAT 
TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC GGTGCATCCA 
GTCGACAATC AGCCACCTTC AATTATAATA GGTAGGACGC TCACGGTGGA CGAAGGCTTC 
TCGGCAGCCC TTACGACTCA TCACTTGACT GCCACTGACT GGGACACTGC TCCGGATGAC 
TTGGAATTCA TTTTGGTTTC TCATCCCCAG TTTGGCTACC TAGAAAACAC ACTGCCCTCT 
GCAGGCTTTG AGAAAAGGAA TACGGGCATA AGCATAGCTT CATTCCAGTG GAGAGACATG 
AAAGCTTCGC ATATTAACTA TGTGCAGTCC AGGCACCTGA GGGAGGAGCC AACTGCTGAT 
GAGTTCCTGG TGTACGCCAC AGATGGGAAG CAACGCTCCT TGGAGACCAC ATTTCACGTT 
ATCATCAACC CCACAAATGA TGAAGCTCCT GATTTTGTGG TACAGAATAT CACTGTGTAC 
GAGGGCCACA TGGTAGAGCT GGATTCTGCC ATCATCAGTG CTACTGACAG GGATGTTCCC 
AAGGACCCCT TGCTCTTCAA CATCACGCAC AAGCCCAAGC ATGGCCTCCT CCTCATCTCG 
GCCACCAGCA AGGATGCTCA TAATGAAATT CACAACTTCT CCATGGAACT GCTCCAGAAT 
GGAATGAAGC TGGCGTATGC CCACGATGAC TCGGAGAGCT CTGCTGACAA CTTCGTGATC 
CAGTTATCAG ACGGGAAACA TAAAATCCTT AAAACCGTTT CAGTAAGCAT CACCCCAGTG 
AACGACGAGA CACCAACACT AAGCAAGAAG GCTGAAGTCT CAGTGACTGT GGGCGACACT 
CGTGTTCTGT CTAGTGCTGT TCTTTCAGCC ATAGATAAAG ACTCACCCAG GGAGAAGATT 
CACTACTTAT TTGAAAGACT TCCCCAAAAT GGGCAACTTC AACTTAAGAT AGGGAGGGAC 
TGGGTCCCTC TCTCCGCTGG CATGCAGTGC ACCCAGGAGG ATGTGGACTT GAACTTGCTA 
AGATACGCGC ATGCTGGTAA AATGCATTCC CAGGATGGAG ACCGCTTTAC CTTCTACCTC 
TGGGACGGGG ACAACAGGTC ACCTGCATTT GACTGTCACA TCATCATTGA AGACACAGGA 
AGAGGTGATA TTGTCATTCA TGCGAAACCA CTCGTGGTAG CCAACGGTGA CAGAGGTCTC 
TTGACGCCTG CCACTCTTCT GGCTGAGGAT GGAGCAGACA AGGCTGAGGA ATTACTCTAC 
CTTATCACTT CCCCACCACA GTATGGCCAA GTGGAGTATG TCTACTCTCC TGGAGTCCCC 
ATTACCAGCT TTAGCCAAAT GGACATAGCA GGACAGGCAG TCTGCTACAT ACACAAGAGC 
AGGACCGCTG TCCTCAGTGA CAGTTTCAGA TTTACCATCA GCAATGGACT GCAGACCCAG 
CAGGGGGTGT TTGAGATCAC ACTGCAGGGT GTGGACAGTG CCTTGCCTGT GCTGACCAGG 
AACCGAAGGC TGAGGCTTGT GGAAGGGGCC ATGGGCCTGC TGTCCACGGA TCACCTTCAG 
CTGACTGACC CTGACACACC TCCAGAGAAC CTAACCTTCT TTCTGGTCCA GCTCCCGCGC 
CACGGGTACC TCTACCTGAG AGGGAAAGTA CTTCAGCACA ACTTCACCCA GCAAGATGTA 
GACAGCAGGG TTGTGGCCTA CCAGCATGCA GGAGGCGGTG CCCAGGAGGA CTGCTTTACT 
TTCCTGGCCA CAGACAGGAA GAACCAAGGC TTCGTTGTGG ATGGGAAAGT TCAGAAAGAG 
CCCGTTCTCT TCATTATCCA GGTGGACCAG CTGGATAAGA AGGCGCCTCG GATCACACGC 
TTGCACTCCC CTTCTCAAGT GGGGCTCTTG AAAAATGGCT GCTATGGGAT TTACATCACT 
TCCCATGTGC TAAAGGCGTC TGACCCTGAC ACAGAGGATG ACCAGATCAT CTTTAAGATT 
TTACGAGGCC CGTTGCATGG ACGTCTGGAG AACACGACAA CAGGTGAATT CATCCACGAG 
CAATTTAGCC AGAAGGACTT GAGCCGCAAG ACCATCCTTT ATATCATAAA CCCTTCTTTG 
CAAGTGAATT CAGATATCCT GGAATTTCAG GCCATGGACC CCACAGGGAA CACTGCCCCT 
CCCCAGAGAT TGGAACTGAA ATGGTCATAT ATTGAATGGT CCCAGACTGA ATATGAGGTC 
TGTGAGAATG TGGGCTTACT GACCTTGGAA GTCACCAGAA GGGGATATTC CATGGACTCA 
GCCTATGTGG ACATAGAGGT TAACCAAGTG TCAGCTGTAG TTGGAAAAGA TTTTACTGTC 
ACTCCATCCA AACTGCTTCA ATTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA 
ATTACCTATG ACGGATTAGA GGAAGACGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT 
GTGAATGCGG TTCTTGGCAC ACAGACAAAA GCAGCAGTGA AAATTTTGGA CTCAAAAGGA 
GGACGGTGCC ATCCTTCAAA TTCCTTCAAC CAAAGCAAGC ACAGCACGTG GGGGAAGGGA 
CGTTGGCATC CACTGCCCTC AGGGTCATCC TCACTCACCA CTTCCGGTTC TCCTCATCTG 
GAAAGAAGGC CCCCTCCATC CATCACCAAA GGAGATGCTC TTCGGGGTTT TGATCTCACA 
GACCTCTCTC AAAGGAAGAT GACGACACAG GGGAATGGCA AATCAGTCCT CCCATCCTCT 
GTTTGTAGAA ATGGAACAGA CACCATCTAC AATTATCATG GTATAGTTTC CTTGAAACTG 
GAGGGGGACA GATTCTCAGC TCACAAACGG AAGGCCAAAA TATCCGTCAT TCGTCAGCCA 
CAGAGGACAG TCAAGGTGGC AGAGCTGCCT CTAGCCGATA AGGTAGAACC CACAACCGAC 
TCGAACTTTC TCAGTCAGGG TCTACGGCCC TTGTTTCCAA AGAACTGTTC TGTGGAATTA 
AAGGGACTCT TTCATTTGGA AGAACACACC CACAGATTGT ATCAATGTGA TGGGATCGCC 
TGGAACGTCT GGAGCTCCCA AACCAAGGGG CTGGAGGACA GATCCTGCCC AGTTGGATGG 
CTTCTTCATT CCGACTACTG TCACGTCTTG GTCACAAGGC AGAAAGGCAC CTGGGCCACA 
GCTACCCGAG CTTGCAGAGA ACAGCACCAG GGCACCCTTG TGACCGTATT CTCCAGGAGG 
CACATGCAGT GGCTGTGGGC TATCAGCGGA AGAAAGCCCT TCTGGATAGG CTTGAAGAAC 
CAAGTGCGAA CTGGCCACTG GGAGTGGATT GGAGGTGAAC CTGTTGCTTT CACCAACTGG 
AGGAGAGGGC CCCCTCTGCA CCCGAAGCCT GGAAAGAACT GTGTTTTGGT TCAAAGAAGA 
GGACAATGGC AAACAAAGAA CTGTAGCAAA GGCAAGGCTC ACAATTTTGT GTGCTCAAGG 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761993.1
CDS	145..5733
Translation	MAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITEAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKMELEVYDLFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYHHDDSDTTKDFVAFRIFDGQHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFNITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGGELVYTITRPPCFSSSHRHLDAGKLFMVDSIPKPTKNPTAPGLNSFTQHAVNHMKVAYMPPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQCTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRYQHDGSEVLQDEIFLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVTITPEYIFATDADSNDSELLFLITREPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGGEIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSVPLHKSFPTYQLNITVHPVDNQPPSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGYLENTLPSAGFEKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVIINPTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLISATSKDAHNEIHNFSMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSITPVNDETPTLSKKAEVSVTVGDTRVLSSAVLSAIDKDSPREKIHYLFERLPQNGQLQLKIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDGDRFTFYLWDGDNRSPAFDCHIIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLITSPPQYGQVEYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQGVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL

Target ORF information:

RefSeq Version	XM_008763771.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763771.2

ORF Insert Sequence:

ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATATTGA CCCCTCTGAC AACCTCCGTG 
CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGCTGG TGTTTAACAT CACTGAAGCC 
CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTTACC 
TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CGAACAACAG CCACCCTGAG 
AGGAGACATT ACAAGATGGA GCTGGAGGTG TACGACCTCT TCTTTGAGAG GAGCGCCCCC 
ATCACAGTTC ACATCTCCAT CAGAACAGCA GACACGAATG CACCTCGGGT GTCCTGGAAT 
ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA GTTTCAGATC 
GTGGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGTCT GCAGCATGGA 
CGGCTTACAG TAAGAGAGGG GAAAGGATTT CTCTTCACCG TGGCTGATCT TCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA 
TTCGATGGCC AACACAGCAG CCATCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC 
AGTCCCCCAT TCCTCATAAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG 
ATCCAAGGGT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGTTCCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGATTGATA 
GGATATCCTG TCTCTGGCTT CCTTCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCT TTTGAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ATGGTCCATA TAACTCCAGT AGACGACCAG 
CTTCCCAAGG AAGCTCCCGG CATATCCCGG CATTTGATTG TCAAGGAAAC GGAAGTGGCC 
TACATCACTA AAAAGCACCT ACATTTCCTA GACACGGAAT CGCATGGTGG GGAGCTTGTC 
TACACAATAA CACGCCCTCC ATGCTTCTCC TCCAGCCACA GGCACTTGGA TGCTGGGAAG 
CTGTTCATGG TGGACAGCAT ACCAAAGCCA ACCAAGAATC CCACAGCCCC AGGGCTGAAC 
TCATTTACTC AGCATGCTGT GAACCACATG AAAGTGGCCT ACATGCCACC CATGCAGGAT 
ATAGGTCCCA CACCTCGACA TGTGCAGTTC ACAGTGTCCG TCAGTAACCA GCATGGAGGT 
ACTTTGCATG GAATCTGCTT CAACATCACA GTGCTCCCAG TGGACAATCA GGTTCCTGAG 
GTATTCACCA ACACCCTGAG AGTGGCTGAG GGTGGCCAAT GCACCATCAG CACAGAGCAC 
ATTTTGGTTT CTGATGTGGA CACCCCACTG GACAGCATCT GCCTCTCTCT TCAGGAGATG 
CCCCTGCATG GCGGGGTAGA GCTGGATGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT 
TGGCGAGACC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGATCTGA GGTTCTTCAG 
GATGAGATAT TCTTGGAGGT CACAGACGGC ACAAATTCTG CAGGATTTGT TCTCTACATT 
GAGGTATTCC CTGTGAATGA TGAGCCACCT GTTCTAAAGG CTGACCTCAT TCCCATGATG 
CACTGCTCAG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTTCGC TACTGATGCA 
GACAGCAATG ACTCAGAACT GCTATTTCTG ATTACCCGAG AACCTCGGCA TGGAGTAGTG 
AGAAAAGCTG GACTCCGTGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGAGCTGTG 
GCATACAAAC ATACAGGTGG CGAGATTGGC CTCGAATCTT GTTTTGACAC TGTTGTATTG 
GTGGTTTCGG ATGGTGAAGC TGACCCTTTG GTGAATGGCT GTTGCTACAA TGGACCCGAT 
TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC GGTGCATCCA 
GTCGACAATC AGCCACCTTC AATTATAATA GGTAGGACGC TCACGGTGGA CGAAGGCTTC 
TCGGCAGCCC TTACGACTCA TCACTTGACT GCCACTGACT GGGACACTGC TCCGGATGAC 
TTGGAATTCA TTTTGGTTTC TCATCCCCAG TTTGGCTACC TAGAAAACAC ACTGCCCTCT 
GCAGGCTTTG AGAAAAGGAA TACGGGCATA AGCATAGCTT CATTCCAGTG GAGAGACATG 
AAAGCTTCGC ATATTAACTA TGTGCAGTCC AGGCACCTGA GGGAGGAGCC AACTGCTGAT 
GAGTTCCTGG TGTACGCCAC AGATGGGAAG CAACGCTCCT TGGAGACCAC ATTTCACGTT 
ATCATCAACC CCACAAATGA TGAAGCTCCT GATTTTGTGG TACAGAATAT CACTGTGTAC 
GAGGGCCACA TGGTAGAGCT GGATTCTGCC ATCATCAGTG CTACTGACAG GGATGTTCCC 
AAGGACCCCT TGCTCTTCAA CATCACGCAC AAGCCCAAGC ATGGCCTCCT CCTCATCTCG 
GCCACCAGCA AGGATGCTCA TAATGAAATT CACAACTTCT CCATGGAACT GCTCCAGAAT 
GGAATGAAGC TGGCGTATGC CCACGATGAC TCGGAGAGCT CTGCTGACAA CTTCGTGATC 
CAGTTATCAG ACGGGAAACA TAAAATCCTT AAAACCGTTT CAGTAAGCAT CACCCCAGTG 
AACGACGAGA CACCAACACT AAGCAAGAAG GCTGAAGTCT CAGTGACTGT GGGCGACACT 
CGTGTTCTGT CTAGTGCTGT TCTTTCAGCC ATAGATAAAG ACTCACCCAG GGAGAAGATT 
CACTACTTAT TTGAAAGACT TCCCCAAAAT GGGCAACTTC AACTTAAGAT AGGGAGGGAC 
TGGGTCCCTC TCTCCGCTGG CATGCAGTGC ACCCAGGAGG ATGTGGACTT GAACTTGCTA 
AGATACGCGC ATGCTGGTAA AATGCATTCC CAGGATGGAG ACCGCTTTAC CTTCTACCTC 
TGGGACGGGG ACAACAGGTC ACCTGCATTT GACTGTCACA TCATCATTGA AGACACAGGA 
AGAGGTGATA TTGTCATTCA TGCGAAACCA CTCGTGGTAG CCAACGGTGA CAGAGGTCTC 
TTGACGCCTG CCACTCTTCT GGCTGAGGAT GGAGCAGACA AGGCTGAGGA ATTACTCTAC 
CTTATCACTT CCCCACCACA GTATGGCCAA GTGGAGTATG TCTACTCTCC TGGAGTCCCC 
ATTACCAGCT TTAGCCAAAT GGACATAGCA GGACAGGCAG TCTGCTACAT ACACAAGAGC 
AGGACCGCTG TCCTCAGTGA CAGTTTCAGA TTTACCATCA GCAATGGACT GCAGACCCAG 
CAGGGGGTGT TTGAGATCAC ACTGCAGGGT GTGGACAGTG CCTTGCCTGT GCTGACCAGG 
AACCGAAGGC TGAGGCTTGT GGAAGGGGCC ATGGGCCTGC TGTCCACGGA TCACCTTCAG 
CTGACTGACC CTGACACACC TCCAGAGAAC CTAACCTTCT TTCTGGTCCA GCTCCCGCGC 
CACGGGTACC TCTACCTGAG AGGGAAAGTA CTTCAGCACA ACTTCACCCA GCAAGATGTA 
GACAGCAGGG TTGTGGCCTA CCAGCATGCA GGAGGCGGTG CCCAGGAGGA CTGCTTTACT 
TTCCTGGCCA CAGACAGGAA GAACCAAGGC TTCGTTGTGG ATGGGAAAGT TCAGAAAGAG 
CCCGTTCTCT TCATTATCCA GGTGGACCAG CTGGATAAGA AGGCGCCTCG GATCACACGC 
TTGCACTCCC CTTCTCAAGT GGGGCTCTTG AAAAATGGCT GCTATGGGAT TTACATCACT 
TCCCATGTGC TAAAGGCGTC TGACCCTGAC ACAGAGGATG ACCAGATCAT CTTTAAGATT 
TTACGAGGCC CGTTGCATGG ACGTCTGGAG AACACGACAA CAGGTGAATT CATCCACGAG 
CAATTTAGCC AGAAGGACTT GAGCCGCAAG ACCATCCTTT ATATCATAAA CCCTTCTTTG 
CAAGTGAATT CAGATATCCT GGAATTTCAG GCCATGGACC CCACAGGGAA CACTGCCCCT 
CCCCAGAGAT TGGAACTGAA ATGGTCATAT ATTGAATGGT CCCAGACTGA ATATGAGGTC 
TGTGAGAATG TGGGCTTACT GACCTTGGAA GTCACCAGAA GGGGATATTC CATGGACTCA 
GCCTATGTGG ACATAGAGGT TAACCAAGTG TCAGCTGTAG TTGGAAAAGA TTTTACTGTC 
ACTCCATCCA AACTGCTTCA ATTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA 
ATTACCTATG ACGGATTAGA GGAAGACGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT 
GTGAATGCGG TTCTTGGCAC ACAGACAAAA GCAGCAGTGA AAATTTTGGA CTCAAAAGGA 
GGACGGTGCC ATCCTTCAAA TTCCTTCAAC CAAAGCAAGC ACAGCACGTG GGGGAAGGGA 
CGTTGGCATC CACTGCCCTC AGGGTCATCC TCACTCACCA CTTCCGGTTC TCCTCATCTG 
GAAAGAAGGC CCCCTCCATC CATCACCAAA GGAGATGCTC TTCGGGGTTT TGATCTCACA 
GACCTCTCTC AAAGGAAGAT GACGACACAG GGGAATGGCA AATCAGTCCT CCCATCCTCT 
GTTTGTAGAA ATGGAACAGA CACCATCTAC AATTATCATG GTATAGTTTC CTTGAAACTG 
GAGGGGGACA GATTCTCAGC TCACAAACGG AAGGCCAAAA TATCCGTCAT TCGTCAGCCA 
CAGAGGACAG TCAAGGTGGC AGAGCTGCCT CTAGCCGATA AGGTAGAACC CACAACCGAC 
TCGAACTTTC TCAGTCAGGG TCTACGGCCC TTGTTTCCAA AGAACTGTTC TGTGGAATTA 
AAGGGACTCT TTCATTTGGA AGAACACACC CACAGATTGT ATCAATGTGA TGGGATCGCC 
TGGAACGTCT GGAGCTCCCA AACCAAGGGG CTGGAGGACA GATCCTGCCC AGTTGGATGG 
CTTCTTCATT CCGACTACTG TCACGTCTTG GTCACAAGGC AGAAAGGCAC CTGGGCCACA 
GCTACCCGAG CTTGCAGAGA ACAGCACCAG GGCACCCTTG TGACCGTATT CTCCAGGAGG 
CACATGCAGT GGCTGTGGGC TATCAGCGGA AGAAAGCCCT TCTGGATAGG CTTGAAGAAC 
CAAGTGCGAA CTGGCCACTG GGAGTGGATT GGAGGTGAAC CTGTTGCTTT CACCAACTGG 
AGGAGAGGGC CCCCTCTGCA CCCGAAGCCT GGAAAGAACT GTGTTTTGGT TCAAAGAAGA 
GGACAATGGC AAACAAAGAA CTGTAGCAAA GGCAAGGCTC ACAATTTTGT GTGCTCAAGG 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem1 ( NM_001191757.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178686.1 Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), mRNA.

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CloneID	ORa07566
Clone ID Related Accession (Same CDS sequence)	NM_001191757.1
Accession Version	NM_001191757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	FRAS1-related extracellular matrix protein 1 precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07049037.1. On Jul 17, 2010 this sequence version replaced XM_233112.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCTGTTGT TCTTGCTGCT CCAGCTGCTT 
ATGCTACACC TGGACCCAGC CAATGCCACC TTCATCAGCA TTAACCGGGG CCTGAGGGTG 
ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGTTTGCCAT CCCTAAAGAG 
AAGGACGCTT GCAGAGTGGA GGTGGTGATG AATGAACCCA TAACCCAGAG GGTTGGGAAA 
CTCATTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTACAATA TGTTCACAAT 
GGATGCCCAA TTCTGGACGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA 
GAGACTTTCA TGGAAACCTT CCTCTTGCGG GTCTCTCTTG TGGAACCAGA CTGTAACATC 
ATCCGCCTGA GCAGCAACGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC CCAAGCCATT 
GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACAGCCA GTCTGGACTG CACCATCAGA 
GTAGACCCCG TGAAGACTCA GCTGCCAGCC CATGGCAAGC TGGTTGTGGT GAATCGGAAG 
TCAGAGGGAC CGCGTGGAGA CCAGCCACAC AGCTTTTTCT CTGAGTCACA GCTAGGGGCA 
GAACGGAAGT GTCCGGATGG AAGCTGTACT CTGGAACCGA AGCAAGTGAC AAGTCTGAAA 
GTGAGCTGTG AGGACTTCCT GCTGACAGGG TTTCACTACC AACACATGCA GCCCCCTTCA 
CCCAACATTG ACTACATCCC CATTCAGTTG GATCTCACAG ACAGAAGGAG TAAAACCATT 
TACAAGTCAG AGAGCGCGTG GTTGCCTGTC TTCATCAGAG TTGGCATTCC TAACCAGGCC 
CCAAGGGCTG CTTTCATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ATTGACCCCT 
CTGACAACCT CCGTGCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GCTGGTGTTT 
AACATCACTG AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC 
ATCTCTTCAT TTACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCGAAC 
AACAGCCACC CTGAGAGGAG ACATTACAAG ATGGAGCTGG AGGTGTACGA CCTCTTCTTT 
GAGAGGAGCG CCCCCATCAC AGTTCACATC TCCATCAGAA CAGCAGACAC GAATGCACCT 
CGGGTGTCCT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG 
GAACAGTTTC AGATCGTGGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT 
GGTCTGCAGC ATGGACGGCT TACAGTAAGA GAGGGGAAAG GATTTCTCTT CACCGTGGCT 
GATCTTCAGG CTGGAGTTGT TCGCTATCAT CATGATGACA GTGATACCAC CAAAGACTTT 
GTGGCTTTCA GGATATTCGA TGGCCAACAC AGCAGCCATC ACAAGTTTCC TATCAACATC 
TTGCCTAAAG ATGACAGTCC CCCATTCCTC ATAACCAATG TCGTGATTGA ACTAGAAGAG 
GGGAAGACCA TCTTGATCCA AGGGTCCATG CTGAGAGCTT CAGACATGGA CTCCAGTGAT 
GACTACATCT TCTTCAATAT CACAAAGTTC CCACAGGCTG GGGAGATCAT GAAGAAGCCA 
GGGCCAAGAT TGATAGGATA TCCTGTCTCT GGCTTCCTTC AGAGAGACCT GTTCAGTGGA 
ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCTTTTGA ATTTGTCCTG 
TGGGACAGCC ATGAACCTCC AAATCTCTCA GTGCCACAGG TGGTGATGGT CCATATAACT 
CCAGTAGACG ACCAGCTTCC CAAGGAAGCT CCCGGCATAT CCCGGCATTT GATTGTCAAG 
GAAACGGAAG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAC GGAATCGCAT 
GGTGGGGAGC TTGTCTACAC AATAACACGC CCTCCATGCT TCTCCTCCAG CCACAGGCAC 
TTGGATGCTG GGAAGCTGTT CATGGTGGAC AGCATACCAA AGCCAACCAA GAATCCCACA 
GCCCCAGGGC TGAACTCATT TACTCAGGTA AAAAGATTTG GAAAGACCCT GGCTAACACA 
GTAAGCACAT TGTTGGGGGG CTTGGGCTTG GGTTGCTCCA ACATTGTGAT CTCTGTAAAT 
CAGTGCATAT GTGCATCTCA ATTTGCTTCA CAGCATGCTG TGAACCACAT GAAAGTGGCC 
TACATGCCAC CCATGCAGGA TATAGGTCCC ACACCTCGAC ATGTGCAGTT CACAGTGTCC 
GTCAGTAACC AGCATGGAGG TACTTTGCAT GGAATCTGCT TCAACATCAC AGTGCTCCCA 
GTGGACAATC AGGTTCCTGA GGTATTCACC AACACCCTGA GAGTGGCTGA GGGTGGCCAA 
TGCACCATCA GCACAGAGCA CATTTTGGTT TCTGATGTGG ACACCCCACT GGACAGCATC 
TGCCTCTCTC TTCAGGAGAT GCCCCTGCAT GGCGGGGTAG AGCTGGATGG ATTTCCTCTA 
AACCCAAGAG GCACATTTTC TTGGCGAGAC CTCCATACCT TAAAAGTTAG GTATCAACAT 
GATGGATCTG AGGTTCTTCA GGATGAGATA TTCTTGGAGG TCACAGACGG CACAAATTCT 
GCAGGATTTG TTCTCTACAT TGAGGTATTC CCTGTGAATG ATGAGCCACC TGTTCTAAAG 
GCTGACCTCA TTCCCATGAT GCACTGCTCA GAGGGTGGAG AGGTGACCAT CACCCCTGAA 
TACATTTTCG CTACTGATGC AGACAGCAAT GACTCAGAAC TGCTATTTCT GATTACCCGA 
GAACCTCGGC ATGGAGTAGT GAGAAAAGCT GGACTCCGTG TGGACAGGTT CTCTCAGGGA 
GATGTCATCT CAGGAGCTGT GGCATACAAA CATACAGGTG GCGAGATTGG CCTCGAATCT 
TGTTTTGACA CTGTTGTATT GGTGGTTTCG GATGGTGAAG CTGACCCTTT GGTGAATGGC 
TGTTGCTACA ATGGACCCGA TTCATCTGTT CCTCTTCATA AGTCCTTCCC CACATACCAG 
CTCAACATCA CGGTGCATCC AGTCGACAAT CAGCCACCTT CAATTATAAT AGGTAGGACG 
CTCACGGTGG ACGAAGGCTT CTCGGCAGCC CTTACGACTC ATCACTTGAC TGCCACTGAC 
TGGGACACTG CTCCGGATGA CTTGGAATTC ATTTTGGTTT CTCATCCCCA GTTTGGCTAC 
CTAGAAAACA CACTGCCCTC TGCAGGCTTT GAGAAAAGGA ATACGGGCAT AAGCATAGCT 
TCATTCCAGT GGAGAGACAT GAAAGCTTCG CATATTAACT ATGTGCAGTC CAGGCACCTG 
AGGGAGGAGC CAACTGCTGA TGAGTTCCTG GTGTACGCCA CAGATGGGAA GCAACGCTCC 
TTGGAGACCA CATTTCACGT TATCATCAAC CCCACAAATG ATGAAGCTCC TGATTTTGTG 
GTACAGAATA TCACTGTGTA CGAGGGCCAC ATGGTAGAGC TGGATTCTGC CATCATCAGT 
GCTACTGACA GGGATGTTCC CAAGGACCCC TTGCTCTTCA ACATCACGCA CAAGCCCAAG 
CATGGCCTCC TCCTCATCTC GGCCACCAGC AAGGATGCTC ATAATGAAAT TCACAACTTC 
TCCATGGAAC TGCTCCAGAA TGGAATGAAG CTGGCGTATG CCCACGATGA CTCGGAGAGC 
TCTGCTGACA ACTTCGTGAT CCAGTTATCA GACGGGAAAC ATAAAATCCT TAAAACCGTT 
TCAGTAAGCA TCACCCCAGT GAACGACGAG ACACCAACAC TAAGCAAGAA GGCTGAAGTC 
TCAGTGACTG TGGGCGACAC TCGTGTTCTG TCTAGTGCTG TTCTTTCAGC CATAGATAAA 
GACTCACCCA GGGAGAAGAT TCACTACTTA TTTGAAAGAC TTCCCCAAAA TGGGCAACTT 
CAACTTAAGA TAGGGAGGGA CTGGGTCCCT CTCTCCGCTG GCATGCAGTG CACCCAGGAG 
GATGTGGACT TGAACTTGCT AAGATACGCG CATGCTGGTA AAATGCATTC CCAGGATGGA 
GACCGCTTTA CCTTCTACCT CTGGGACGGG GACAACAGGT CACCTGCATT TGACTGTCAC 
ATCATCATTG AAGACACAGG AAGAGGTGAT ATTGTCATTC ATGCGAAACC ACTCGTGGTA 
GCCAACGGTG ACAGAGGTCT CTTGACGCCT GCCACTCTTC TGGCTGAGGA TGGAGCAGAC 
AAGGCTGAGG AATTACTCTA CCTTATCACT TCCCCACCAC AGTATGGCCA AGTGGAGTAT 
GTCTACTCTC CTGGAGTCCC CATTACCAGC TTTAGCCAAA TGGACATAGC AGGACAGGCA 
GTCTGCTACA TACACAAGAG CAGGACCGCT GTCCTCAGTG ACAGTTTCAG ATTTACCATC 
AGCAATGGAC TGCAGACCCA GCAGGGGGTG TTTGAGATCA CACTGCAGGG TGTGGACAGT 
GCCTTGCCTG TGCTGACCAG GAACCGAAGG CTGAGGCTTG TGGAAGGGGC CATGGGCCTG 
CTGTCCACGG ATCACCTTCA GCTGACTGAC CCTGACACAC CTCCAGAGAA CCTAACCTTC 
TTTCTGGTCC AGCTCCCGCG CCACGGGTAC CTCTACCTGA GAGGGAAAGT ACTTCAGCAC 
AACTTCACCC AGCAAGATGT AGACAGCAGG GTTGTGGCCT ACCAGCATGC AGGAGGCGGT 
GCCCAGGAGG ACTGCTTTAC TTTCCTGGCC ACAGACAGGA AGAACCAAGG CTTCGTTGTG 
GATGGGAAAG TTCAGAAAGA GCCCGTTCTC TTCATTATCC AGGTGGACCA GCTGGATAAG 
AAGGCGCCTC GGATCACACG CTTGCACTCC CCTTCTCAAG TGGGGCTCTT GAAAAATGGC 
TGCTATGGGA TTTACATCAC TTCCCATGTG CTAAAGGCGT CTGACCCTGA CACAGAGGAT 
GACCAGATCA TCTTTAAGAT TTTACGAGGC CCGTTGCATG GACGTCTGGA GAACACGACA 
ACAGGTGAAT TCATCCACGA GCAATTTAGC CAGAAGGACT TGAGCCGCAA GACCATCCTT 
TATATCATAA ACCCTTCTTT GCAAGTGAAT TCAGATATCC TGGAATTTCA GGCCATGGAC 
CCCACAGGGA ACACTGCCCC TCCCCAGAGA TTGGAACTGA AATGGTCATA TATTGAATGG 
TCCCAGACTG AATATGAGGT CTGTGAGAAT GTGGGCTTAC TGACCTTGGA AGTCACCAGA 
AGGGGATATT CCATGGACTC AGCCTATGTG GACATAGAGG TTAACCAAGT GTCAGCTGTA 
GTTGGAAAAG ATTTTACTGT CACTCCATCC AAACTGCTTC AATTTGACCC AGGAATGTCA 
ACTAAGATGT GGAATATAGC AATTACCTAT GACGGATTAG AGGAAGACGA TGAGGTCTTT 
GAAGTAATTC TGAACTCCCC TGTGAATGCG GTTCTTGGCA CACAGACAAA AGCAGCAGTG 
AAAATTTTGG ACTCAAAAGG AGGACGGTGC CATCCTTCAA ATTCCTTCAA CCAAAGCAAG 
CACAGCACGT GGGGGAAGGG ACGTTGGCAT CCACTGCCCT CAGGGTCATC CTCACTCACC 
ACTTCCGGTT CTCCTCATCT GGAAAGAAGG CCCCCTCCAT CCATCACCAA AGGAGATGCT 
CTTCGGGGTT TTGATCTCAC AGACCTCTCT CAAAGGAAGA TGACGACACA GGGGAATGGC 
AAATCAGTCC TCCCATCCTC TGTTTGTAGA AATGGAACAG ACACCATCTA CAATTATCAT 
GGTATAGTTT CCTTGAAACT GGAGGGGGAC AGATTCTCAG CTCACAAACG GAAGGCCAAA 
ATATCCGTCA TTCGTCAGCC ACAGAGGACA GTCAAGGTGG CAGAGCTGCC TCTAGCCGAT 
AAGGTAGAAC CCACAACCGA CTCGAACTTT CTCAGTCAGG GTCTACGGCC CTTGTTTCCA 
AAGAACTGTT CTGTGGAATT AAAGGGACTC TTTCATTTGG AAGAACACAC CCACAGATTG 
TATCAATGTG ATGGGATCGC CTGGAACGTC TGGAGCTCCC AAACCAAGGG GCTGGAGGAC 
AGATCCTGCC CAGTTGGATG GCTTCTTCAT TCCGACTACT GTCACGTCTT GGTCACAAGG 
CAGAAAGGCA CCTGGGCCAC AGCTACCCGA GCTTGCAGAG AACAGCACCA GGGCACCCTT 
GTGACCGTAT TCTCCAGGAG GCACATGCAG TGGCTGTGGG CTATCAGCGG AAGAAAGCCC 
TTCTGGATAG GCTTGAAGAA CCAAGTGCGA ACTGGCCACT GGGAGTGGAT TGGAGGTGAA 
CCTGTTGCTT TCACCAACTG GAGGAGAGGG CCCCCTCTGC ACCCGAAGCC TGGAAAGAAC 
TGTGTTTTGG TTCAAAGAAG AGGACAATGG CAAACAAAGA ACTGTAGCAA AGGCAAGGCT 
CACAATTTTG TGTGCTCAAG GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178686.1
CDS	1..6630
Translation	MHSPGCTGPKALLFLLLQLLMLHLDPANATFISINRGLRVMKGSSAFLSGDHLRFAIPKEKDACRVEVVMNEPITQRVGKLIPQVFDCHFLPNEVQYVHNGCPILDEDSVKLRLYRFTETETFMETFLLRVSLVEPDCNIIRLSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRVDPVKTQLPAHGKLVVVNRKSEGPRGDQPHSFFSESQLGAERKCPDGSCTLEPKQVTSLKVSCEDFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTIYKSESAWLPVFIRVGIPNQAPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITEAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKMELEVYDLFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYHHDDSDTTKDFVAFRIFDGQHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFNITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGGELVYTITRPPCFSSSHRHLDAGKLFMVDSIPKPTKNPTAPGLNSFTQVKRFGKTLANTVSTLLGGLGLGCSNIVISVNQCICASQFASQHAVNHMKVAYMPPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQCTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRYQHDGSEVLQDEIFLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVTITPEYIFATDADSNDSELLFLITREPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGGEIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSVPLHKSFPTYQLNITVHPVDNQPPSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGYLENTLPSAGFEKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVIINPTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLISATSKDAHNEIHNFSMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSITPVNDETPTLSKKAEVSVTVGDTRVLSSAVLSAIDKDSPREKIHYLFERLPQNGQLQLKIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDGDRFTFYLWDGDNRSPAFDCHIIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLITSPPQYGQVEYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQGVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL

Target ORF information:

RefSeq Version	NM_001191757.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191757.1

ORF Insert Sequence:

ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCTGTTGT TCTTGCTGCT CCAGCTGCTT 
ATGCTACACC TGGACCCAGC CAATGCCACC TTCATCAGCA TTAACCGGGG CCTGAGGGTG 
ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGTTTGCCAT CCCTAAAGAG 
AAGGACGCTT GCAGAGTGGA GGTGGTGATG AATGAACCCA TAACCCAGAG GGTTGGGAAA 
CTCATTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTACAATA TGTTCACAAT 
GGATGCCCAA TTCTGGACGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA 
GAGACTTTCA TGGAAACCTT CCTCTTGCGG GTCTCTCTTG TGGAACCAGA CTGTAACATC 
ATCCGCCTGA GCAGCAACGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC CCAAGCCATT 
GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACAGCCA GTCTGGACTG CACCATCAGA 
GTAGACCCCG TGAAGACTCA GCTGCCAGCC CATGGCAAGC TGGTTGTGGT GAATCGGAAG 
TCAGAGGGAC CGCGTGGAGA CCAGCCACAC AGCTTTTTCT CTGAGTCACA GCTAGGGGCA 
GAACGGAAGT GTCCGGATGG AAGCTGTACT CTGGAACCGA AGCAAGTGAC AAGTCTGAAA 
GTGAGCTGTG AGGACTTCCT GCTGACAGGG TTTCACTACC AACACATGCA GCCCCCTTCA 
CCCAACATTG ACTACATCCC CATTCAGTTG GATCTCACAG ACAGAAGGAG TAAAACCATT 
TACAAGTCAG AGAGCGCGTG GTTGCCTGTC TTCATCAGAG TTGGCATTCC TAACCAGGCC 
CCAAGGGCTG CTTTCATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ATTGACCCCT 
CTGACAACCT CCGTGCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GCTGGTGTTT 
AACATCACTG AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC 
ATCTCTTCAT TTACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCGAAC 
AACAGCCACC CTGAGAGGAG ACATTACAAG ATGGAGCTGG AGGTGTACGA CCTCTTCTTT 
GAGAGGAGCG CCCCCATCAC AGTTCACATC TCCATCAGAA CAGCAGACAC GAATGCACCT 
CGGGTGTCCT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG 
GAACAGTTTC AGATCGTGGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT 
GGTCTGCAGC ATGGACGGCT TACAGTAAGA GAGGGGAAAG GATTTCTCTT CACCGTGGCT 
GATCTTCAGG CTGGAGTTGT TCGCTATCAT CATGATGACA GTGATACCAC CAAAGACTTT 
GTGGCTTTCA GGATATTCGA TGGCCAACAC AGCAGCCATC ACAAGTTTCC TATCAACATC 
TTGCCTAAAG ATGACAGTCC CCCATTCCTC ATAACCAATG TCGTGATTGA ACTAGAAGAG 
GGGAAGACCA TCTTGATCCA AGGGTCCATG CTGAGAGCTT CAGACATGGA CTCCAGTGAT 
GACTACATCT TCTTCAATAT CACAAAGTTC CCACAGGCTG GGGAGATCAT GAAGAAGCCA 
GGGCCAAGAT TGATAGGATA TCCTGTCTCT GGCTTCCTTC AGAGAGACCT GTTCAGTGGA 
ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCTTTTGA ATTTGTCCTG 
TGGGACAGCC ATGAACCTCC AAATCTCTCA GTGCCACAGG TGGTGATGGT CCATATAACT 
CCAGTAGACG ACCAGCTTCC CAAGGAAGCT CCCGGCATAT CCCGGCATTT GATTGTCAAG 
GAAACGGAAG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAC GGAATCGCAT 
GGTGGGGAGC TTGTCTACAC AATAACACGC CCTCCATGCT TCTCCTCCAG CCACAGGCAC 
TTGGATGCTG GGAAGCTGTT CATGGTGGAC AGCATACCAA AGCCAACCAA GAATCCCACA 
GCCCCAGGGC TGAACTCATT TACTCAGGTA AAAAGATTTG GAAAGACCCT GGCTAACACA 
GTAAGCACAT TGTTGGGGGG CTTGGGCTTG GGTTGCTCCA ACATTGTGAT CTCTGTAAAT 
CAGTGCATAT GTGCATCTCA ATTTGCTTCA CAGCATGCTG TGAACCACAT GAAAGTGGCC 
TACATGCCAC CCATGCAGGA TATAGGTCCC ACACCTCGAC ATGTGCAGTT CACAGTGTCC 
GTCAGTAACC AGCATGGAGG TACTTTGCAT GGAATCTGCT TCAACATCAC AGTGCTCCCA 
GTGGACAATC AGGTTCCTGA GGTATTCACC AACACCCTGA GAGTGGCTGA GGGTGGCCAA 
TGCACCATCA GCACAGAGCA CATTTTGGTT TCTGATGTGG ACACCCCACT GGACAGCATC 
TGCCTCTCTC TTCAGGAGAT GCCCCTGCAT GGCGGGGTAG AGCTGGATGG ATTTCCTCTA 
AACCCAAGAG GCACATTTTC TTGGCGAGAC CTCCATACCT TAAAAGTTAG GTATCAACAT 
GATGGATCTG AGGTTCTTCA GGATGAGATA TTCTTGGAGG TCACAGACGG CACAAATTCT 
GCAGGATTTG TTCTCTACAT TGAGGTATTC CCTGTGAATG ATGAGCCACC TGTTCTAAAG 
GCTGACCTCA TTCCCATGAT GCACTGCTCA GAGGGTGGAG AGGTGACCAT CACCCCTGAA 
TACATTTTCG CTACTGATGC AGACAGCAAT GACTCAGAAC TGCTATTTCT GATTACCCGA 
GAACCTCGGC ATGGAGTAGT GAGAAAAGCT GGACTCCGTG TGGACAGGTT CTCTCAGGGA 
GATGTCATCT CAGGAGCTGT GGCATACAAA CATACAGGTG GCGAGATTGG CCTCGAATCT 
TGTTTTGACA CTGTTGTATT GGTGGTTTCG GATGGTGAAG CTGACCCTTT GGTGAATGGC 
TGTTGCTACA ATGGACCCGA TTCATCTGTT CCTCTTCATA AGTCCTTCCC CACATACCAG 
CTCAACATCA CGGTGCATCC AGTCGACAAT CAGCCACCTT CAATTATAAT AGGTAGGACG 
CTCACGGTGG ACGAAGGCTT CTCGGCAGCC CTTACGACTC ATCACTTGAC TGCCACTGAC 
TGGGACACTG CTCCGGATGA CTTGGAATTC ATTTTGGTTT CTCATCCCCA GTTTGGCTAC 
CTAGAAAACA CACTGCCCTC TGCAGGCTTT GAGAAAAGGA ATACGGGCAT AAGCATAGCT 
TCATTCCAGT GGAGAGACAT GAAAGCTTCG CATATTAACT ATGTGCAGTC CAGGCACCTG 
AGGGAGGAGC CAACTGCTGA TGAGTTCCTG GTGTACGCCA CAGATGGGAA GCAACGCTCC 
TTGGAGACCA CATTTCACGT TATCATCAAC CCCACAAATG ATGAAGCTCC TGATTTTGTG 
GTACAGAATA TCACTGTGTA CGAGGGCCAC ATGGTAGAGC TGGATTCTGC CATCATCAGT 
GCTACTGACA GGGATGTTCC CAAGGACCCC TTGCTCTTCA ACATCACGCA CAAGCCCAAG 
CATGGCCTCC TCCTCATCTC GGCCACCAGC AAGGATGCTC ATAATGAAAT TCACAACTTC 
TCCATGGAAC TGCTCCAGAA TGGAATGAAG CTGGCGTATG CCCACGATGA CTCGGAGAGC 
TCTGCTGACA ACTTCGTGAT CCAGTTATCA GACGGGAAAC ATAAAATCCT TAAAACCGTT 
TCAGTAAGCA TCACCCCAGT GAACGACGAG ACACCAACAC TAAGCAAGAA GGCTGAAGTC 
TCAGTGACTG TGGGCGACAC TCGTGTTCTG TCTAGTGCTG TTCTTTCAGC CATAGATAAA 
GACTCACCCA GGGAGAAGAT TCACTACTTA TTTGAAAGAC TTCCCCAAAA TGGGCAACTT 
CAACTTAAGA TAGGGAGGGA CTGGGTCCCT CTCTCCGCTG GCATGCAGTG CACCCAGGAG 
GATGTGGACT TGAACTTGCT AAGATACGCG CATGCTGGTA AAATGCATTC CCAGGATGGA 
GACCGCTTTA CCTTCTACCT CTGGGACGGG GACAACAGGT CACCTGCATT TGACTGTCAC 
ATCATCATTG AAGACACAGG AAGAGGTGAT ATTGTCATTC ATGCGAAACC ACTCGTGGTA 
GCCAACGGTG ACAGAGGTCT CTTGACGCCT GCCACTCTTC TGGCTGAGGA TGGAGCAGAC 
AAGGCTGAGG AATTACTCTA CCTTATCACT TCCCCACCAC AGTATGGCCA AGTGGAGTAT 
GTCTACTCTC CTGGAGTCCC CATTACCAGC TTTAGCCAAA TGGACATAGC AGGACAGGCA 
GTCTGCTACA TACACAAGAG CAGGACCGCT GTCCTCAGTG ACAGTTTCAG ATTTACCATC 
AGCAATGGAC TGCAGACCCA GCAGGGGGTG TTTGAGATCA CACTGCAGGG TGTGGACAGT 
GCCTTGCCTG TGCTGACCAG GAACCGAAGG CTGAGGCTTG TGGAAGGGGC CATGGGCCTG 
CTGTCCACGG ATCACCTTCA GCTGACTGAC CCTGACACAC CTCCAGAGAA CCTAACCTTC 
TTTCTGGTCC AGCTCCCGCG CCACGGGTAC CTCTACCTGA GAGGGAAAGT ACTTCAGCAC 
AACTTCACCC AGCAAGATGT AGACAGCAGG GTTGTGGCCT ACCAGCATGC AGGAGGCGGT 
GCCCAGGAGG ACTGCTTTAC TTTCCTGGCC ACAGACAGGA AGAACCAAGG CTTCGTTGTG 
GATGGGAAAG TTCAGAAAGA GCCCGTTCTC TTCATTATCC AGGTGGACCA GCTGGATAAG 
AAGGCGCCTC GGATCACACG CTTGCACTCC CCTTCTCAAG TGGGGCTCTT GAAAAATGGC 
TGCTATGGGA TTTACATCAC TTCCCATGTG CTAAAGGCGT CTGACCCTGA CACAGAGGAT 
GACCAGATCA TCTTTAAGAT TTTACGAGGC CCGTTGCATG GACGTCTGGA GAACACGACA 
ACAGGTGAAT TCATCCACGA GCAATTTAGC CAGAAGGACT TGAGCCGCAA GACCATCCTT 
TATATCATAA ACCCTTCTTT GCAAGTGAAT TCAGATATCC TGGAATTTCA GGCCATGGAC 
CCCACAGGGA ACACTGCCCC TCCCCAGAGA TTGGAACTGA AATGGTCATA TATTGAATGG 
TCCCAGACTG AATATGAGGT CTGTGAGAAT GTGGGCTTAC TGACCTTGGA AGTCACCAGA 
AGGGGATATT CCATGGACTC AGCCTATGTG GACATAGAGG TTAACCAAGT GTCAGCTGTA 
GTTGGAAAAG ATTTTACTGT CACTCCATCC AAACTGCTTC AATTTGACCC AGGAATGTCA 
ACTAAGATGT GGAATATAGC AATTACCTAT GACGGATTAG AGGAAGACGA TGAGGTCTTT 
GAAGTAATTC TGAACTCCCC TGTGAATGCG GTTCTTGGCA CACAGACAAA AGCAGCAGTG 
AAAATTTTGG ACTCAAAAGG AGGACGGTGC CATCCTTCAA ATTCCTTCAA CCAAAGCAAG 
CACAGCACGT GGGGGAAGGG ACGTTGGCAT CCACTGCCCT CAGGGTCATC CTCACTCACC 
ACTTCCGGTT CTCCTCATCT GGAAAGAAGG CCCCCTCCAT CCATCACCAA AGGAGATGCT 
CTTCGGGGTT TTGATCTCAC AGACCTCTCT CAAAGGAAGA TGACGACACA GGGGAATGGC 
AAATCAGTCC TCCCATCCTC TGTTTGTAGA AATGGAACAG ACACCATCTA CAATTATCAT 
GGTATAGTTT CCTTGAAACT GGAGGGGGAC AGATTCTCAG CTCACAAACG GAAGGCCAAA 
ATATCCGTCA TTCGTCAGCC ACAGAGGACA GTCAAGGTGG CAGAGCTGCC TCTAGCCGAT 
AAGGTAGAAC CCACAACCGA CTCGAACTTT CTCAGTCAGG GTCTACGGCC CTTGTTTCCA 
AAGAACTGTT CTGTGGAATT AAAGGGACTC TTTCATTTGG AAGAACACAC CCACAGATTG 
TATCAATGTG ATGGGATCGC CTGGAACGTC TGGAGCTCCC AAACCAAGGG GCTGGAGGAC 
AGATCCTGCC CAGTTGGATG GCTTCTTCAT TCCGACTACT GTCACGTCTT GGTCACAAGG 
CAGAAAGGCA CCTGGGCCAC AGCTACCCGA GCTTGCAGAG AACAGCACCA GGGCACCCTT 
GTGACCGTAT TCTCCAGGAG GCACATGCAG TGGCTGTGGG CTATCAGCGG AAGAAAGCCC 
TTCTGGATAG GCTTGAAGAA CCAAGTGCGA ACTGGCCACT GGGAGTGGAT TGGAGGTGAA 
CCTGTTGCTT TCACCAACTG GAGGAGAGGG CCCCCTCTGC ACCCGAAGCC TGGAAAGAAC 
TGTGTTTTGG TTCAAAGAAG AGGACAATGG CAAACAAAGA ACTGTAGCAA AGGCAAGGCT 
CACAATTTTG TGTGCTCAAG GAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem1 ( XM_006238349.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238411.1 Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X1, mRNA.

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CloneID	ORa28279
Clone ID Related Accession (Same CDS sequence)	XM_006238349.3
Accession Version	XM_006238349.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238349.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCTGTTGT TCTTGCTGCT CCAGCTGCTT 
ATGCTACACC TGGACCCAGC CAATGCCACC TTCATCAGCA TTAACCGGGG CCTGAGGGTG 
ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGTTTGCCAT CCCTAAAGAG 
AAGGACGCTT GCAGAGTGGA GGTGGTGATG AATGAACCCA TAACCCAGAG GGTTGGGAAA 
CTCATTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTACAATA TGTTCACAAT 
GGATGCCCAA TTCTGGACGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA 
GAGACTTTCA TGGAAACCTT CCTCTTGCGG GTCTCTCTTG TGGAACCAGA CTGTAACATC 
ATCCGCCTGA GCAGCAACGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC CCAAGCCATT 
GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACAGCCA GTCTGGACTG CACCATCAGA 
GTAGACCCCG TGAAGACTCA GCTGCCAGCC CATGGCAAGC TGGTTGTGGT GAATCGGAAG 
TCAGAGGGAC CGCGTGGAGA CCAGCCACAC AGCTTTTTCT CTGAGTCACA GCTAGGGGCA 
GAACGGAAGT GTCCGGATGG AAGCTGTACT CTGGAACCGA AGCAAGTGAC AAGTCTGAAA 
GTGAGCTGTG AGGACTTCCT GCTGACAGGG TTTCACTACC AACACATGCA GCCCCCTTCA 
CCCAACATTG ACTACATCCC CATTCAGTTG GATCTCACAG ACAGAAGGAG TAAAACCATT 
TACAAGTCAG AGAGCGCGTG GTTGCCTGTC TTCATCAGAG TTGGCATTCC TAACCAGGCC 
CCAAGGGCTG CTTTCATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ATTGACCCCT 
CTGACAACCT CCGTGCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GCTGGTGTTT 
AACATCACTG AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC 
ATCTCTTCAT TTACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCGAAC 
AACAGCCACC CTGAGAGGAG ACATTACAAG ATGGAGCTGG AGGTGTACGA CCTCTTCTTT 
GAGAGGAGCG CCCCCATCAC AGTTCACATC TCCATCAGAA CAGCAGACAC GAATGCACCT 
CGGGTGTCCT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG 
GAACAGTTTC AGATCGTGGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT 
GGTCTGCAGC ATGGACGGCT TACAGTAAGA GAGGGGAAAG GATTTCTCTT CACCGTGGCT 
GATCTTCAGG CTGGAGTTGT TCGCTATCAT CATGATGACA GTGATACCAC CAAAGACTTT 
GTGGCTTTCA GGATATTCGA TGGCCAACAC AGCAGCCATC ACAAGTTTCC TATCAACATC 
TTGCCTAAAG ATGACAGTCC CCCATTCCTC ATAACCAATG TCGTGATTGA ACTAGAAGAG 
GGGAAGACCA TCTTGATCCA AGGGTCCATG CTGAGAGCTT CAGACATGGA CTCCAGTGAT 
GACTACATCT TCTTCAATAT CACAAAGTTC CCACAGGCTG GGGAGATCAT GAAGAAGCCA 
GGGCCAAGAT TGATAGGATA TCCTGTCTCT GGCTTCCTTC AGAGAGACCT GTTCAGTGGA 
ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCTTTTGA ATTTGTCCTG 
TGGGACAGCC ATGAACCTCC AAATCTCTCA GTGCCACAGG TGGTGATGGT CCATATAACT 
CCAGTAGACG ACCAGCTTCC CAAGGAAGCT CCCGGCATAT CCCGGCATTT GATTGTCAAG 
GAAACGGAAG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAC GGAATCGCAT 
GGTGGGGAGC TTGTCTACAC AATAACACGC CCTCCATGCT TCTCCTCCAG CCACAGGCAC 
TTGGATGCTG GGAAGCTGTT CATGGTGGAC AGCATACCAA AGCCAACCAA GAATCCCACA 
GCCCCAGGGC TGAACTCATT TACTCAGCAT GCTGTGAACC ACATGAAAGT GGCCTACATG 
CCACCCATGC AGGATATAGG TCCCACACCT CGACATGTGC AGTTCACAGT GTCCGTCAGT 
AACCAGCATG GAGGTACTTT GCATGGAATC TGCTTCAACA TCACAGTGCT CCCAGTGGAC 
AATCAGGTTC CTGAGGTATT CACCAACACC CTGAGAGTGG CTGAGGGTGG CCAATGCACC 
ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CACTGGACAG CATCTGCCTC 
TCTCTTCAGG AGATGCCCCT GCATGGCGGG GTAGAGCTGG ATGGATTTCC TCTAAACCCA 
AGAGGCACAT TTTCTTGGCG AGACCTCCAT ACCTTAAAAG TTAGGTATCA ACATGATGGA 
TCTGAGGTTC TTCAGGATGA GATATTCTTG GAGGTCACAG ACGGCACAAA TTCTGCAGGA 
TTTGTTCTCT ACATTGAGGT ATTCCCTGTG AATGATGAGC CACCTGTTCT AAAGGCTGAC 
CTCATTCCCA TGATGCACTG CTCAGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT 
TTCGCTACTG ATGCAGACAG CAATGACTCA GAACTGCTAT TTCTGATTAC CCGAGAACCT 
CGGCATGGAG TAGTGAGAAA AGCTGGACTC CGTGTGGACA GGTTCTCTCA GGGAGATGTC 
ATCTCAGGAG CTGTGGCATA CAAACATACA GGTGGCGAGA TTGGCCTCGA ATCTTGTTTT 
GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGGTGAA TGGCTGTTGC 
TACAATGGAC CCGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC 
ATCACGGTGC ATCCAGTCGA CAATCAGCCA CCTTCAATTA TAATAGGTAG GACGCTCACG 
GTGGACGAAG GCTTCTCGGC AGCCCTTACG ACTCATCACT TGACTGCCAC TGACTGGGAC 
ACTGCTCCGG ATGACTTGGA ATTCATTTTG GTTTCTCATC CCCAGTTTGG CTACCTAGAA 
AACACACTGC CCTCTGCAGG CTTTGAGAAA AGGAATACGG GCATAAGCAT AGCTTCATTC 
CAGTGGAGAG ACATGAAAGC TTCGCATATT AACTATGTGC AGTCCAGGCA CCTGAGGGAG 
GAGCCAACTG CTGATGAGTT CCTGGTGTAC GCCACAGATG GGAAGCAACG CTCCTTGGAG 
ACCACATTTC ACGTTATCAT CAACCCCACA AATGATGAAG CTCCTGATTT TGTGGTACAG 
AATATCACTG TGTACGAGGG CCACATGGTA GAGCTGGATT CTGCCATCAT CAGTGCTACT 
GACAGGGATG TTCCCAAGGA CCCCTTGCTC TTCAACATCA CGCACAAGCC CAAGCATGGC 
CTCCTCCTCA TCTCGGCCAC CAGCAAGGAT GCTCATAATG AAATTCACAA CTTCTCCATG 
GAACTGCTCC AGAATGGAAT GAAGCTGGCG TATGCCCACG ATGACTCGGA GAGCTCTGCT 
GACAACTTCG TGATCCAGTT ATCAGACGGG AAACATAAAA TCCTTAAAAC CGTTTCAGTA 
AGCATCACCC CAGTGAACGA CGAGACACCA ACACTAAGCA AGAAGGCTGA AGTCTCAGTG 
ACTGTGGGCG ACACTCGTGT TCTGTCTAGT GCTGTTCTTT CAGCCATAGA TAAAGACTCA 
CCCAGGGAGA AGATTCACTA CTTATTTGAA AGACTTCCCC AAAATGGGCA ACTTCAACTT 
AAGATAGGGA GGGACTGGGT CCCTCTCTCC GCTGGCATGC AGTGCACCCA GGAGGATGTG 
GACTTGAACT TGCTAAGATA CGCGCATGCT GGTAAAATGC ATTCCCAGGA TGGAGACCGC 
TTTACCTTCT ACCTCTGGGA CGGGGACAAC AGGTCACCTG CATTTGACTG TCACATCATC 
ATTGAAGACA CAGGAAGAGG TGATATTGTC ATTCATGCGA AACCACTCGT GGTAGCCAAC 
GGTGACAGAG GTCTCTTGAC GCCTGCCACT CTTCTGGCTG AGGATGGAGC AGACAAGGCT 
GAGGAATTAC TCTACCTTAT CACTTCCCCA CCACAGTATG GCCAAGTGGA GTATGTCTAC 
TCTCCTGGAG TCCCCATTAC CAGCTTTAGC CAAATGGACA TAGCAGGACA GGCAGTCTGC 
TACATACACA AGAGCAGGAC CGCTGTCCTC AGTGACAGTT TCAGATTTAC CATCAGCAAT 
GGACTGCAGA CCCAGCAGGG GGTGTTTGAG ATCACACTGC AGGGTGTGGA CAGTGCCTTG 
CCTGTGCTGA CCAGGAACCG AAGGCTGAGG CTTGTGGAAG GGGCCATGGG CCTGCTGTCC 
ACGGATCACC TTCAGCTGAC TGACCCTGAC ACACCTCCAG AGAACCTAAC CTTCTTTCTG 
GTCCAGCTCC CGCGCCACGG GTACCTCTAC CTGAGAGGGA AAGTACTTCA GCACAACTTC 
ACCCAGCAAG ATGTAGACAG CAGGGTTGTG GCCTACCAGC ATGCAGGAGG CGGTGCCCAG 
GAGGACTGCT TTACTTTCCT GGCCACAGAC AGGAAGAACC AAGGCTTCGT TGTGGATGGG 
AAAGTTCAGA AAGAGCCCGT TCTCTTCATT ATCCAGGTGG ACCAGCTGGA TAAGAAGGCG 
CCTCGGATCA CACGCTTGCA CTCCCCTTCT CAAGTGGGGC TCTTGAAAAA TGGCTGCTAT 
GGGATTTACA TCACTTCCCA TGTGCTAAAG GCGTCTGACC CTGACACAGA GGATGACCAG 
ATCATCTTTA AGATTTTACG AGGCCCGTTG CATGGACGTC TGGAGAACAC GACAACAGGT 
GAATTCATCC ACGAGCAATT TAGCCAGAAG GACTTGAGCC GCAAGACCAT CCTTTATATC 
ATAAACCCTT CTTTGCAAGT GAATTCAGAT ATCCTGGAAT TTCAGGCCAT GGACCCCACA 
GGGAACACTG CCCCTCCCCA GAGATTGGAA CTGAAATGGT CATATATTGA ATGGTCCCAG 
ACTGAATATG AGGTCTGTGA GAATGTGGGC TTACTGACCT TGGAAGTCAC CAGAAGGGGA 
TATTCCATGG ACTCAGCCTA TGTGGACATA GAGGTTAACC AAGTGTCAGC TGTAGTTGGA 
AAAGATTTTA CTGTCACTCC ATCCAAACTG CTTCAATTTG ACCCAGGAAT GTCAACTAAG 
ATGTGGAATA TAGCAATTAC CTATGACGGA TTAGAGGAAG ACGATGAGGT CTTTGAAGTA 
ATTCTGAACT CCCCTGTGAA TGCGGTTCTT GGCACACAGA CAAAAGCAGC AGTGAAAATT 
TTGGACTCAA AAGGAGGACG GTGCCATCCT TCAAATTCCT TCAACCAAAG CAAGCACAGC 
ACGTGGGGGA AGGGACGTTG GCATCCACTG CCCTCAGGGT CATCCTCACT CACCACTTCC 
GGTTCTCCTC ATCTGGAAAG AAGGCCCCCT CCATCCATCA CCAAAGGAGA TGCTCTTCGG 
GGTTTTGATC TCACAGACCT CTCTCAAAGG AAGATGACGA CACAGGGGAA TGGCAAATCA 
GTCCTCCCAT CCTCTGTTTG TAGAAATGGA ACAGACACCA TCTACAATTA TCATGGTATA 
GTTTCCTTGA AACTGGAGGG GGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC 
GTCATTCGTC AGCCACAGAG GACAGTCAAG GTGGCAGAGC TGCCTCTAGC CGATAAGGTA 
GAACCCACAA CCGACTCGAA CTTTCTCAGT CAGGGTCTAC GGCCCTTGTT TCCAAAGAAC 
TGTTCTGTGG AATTAAAGGG ACTCTTTCAT TTGGAAGAAC ACACCCACAG ATTGTATCAA 
TGTGATGGGA TCGCCTGGAA CGTCTGGAGC TCCCAAACCA AGGGGCTGGA GGACAGATCC 
TGCCCAGTTG GATGGCTTCT TCATTCCGAC TACTGTCACG TCTTGGTCAC AAGGCAGAAA 
GGCACCTGGG CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCAC CCTTGTGACC 
GTATTCTCCA GGAGGCACAT GCAGTGGCTG TGGGCTATCA GCGGAAGAAA GCCCTTCTGG 
ATAGGCTTGA AGAACCAAGT GCGAACTGGC CACTGGGAGT GGATTGGAGG TGAACCTGTT 
GCTTTCACCA ACTGGAGGAG AGGGCCCCCT CTGCACCCGA AGCCTGGAAA GAACTGTGTT 
TTGGTTCAAA GAAGAGGACA ATGGCAAACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT 
TTTGTGTGCT CAAGGAAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238411.1
CDS	720..7223
Translation	MHSPGCTGPKALLFLLLQLLMLHLDPANATFISINRGLRVMKGSSAFLSGDHLRFAIPKEKDACRVEVVMNEPITQRVGKLIPQVFDCHFLPNEVQYVHNGCPILDEDSVKLRLYRFTETETFMETFLLRVSLVEPDCNIIRLSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRVDPVKTQLPAHGKLVVVNRKSEGPRGDQPHSFFSESQLGAERKCPDGSCTLEPKQVTSLKVSCEDFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTIYKSESAWLPVFIRVGIPNQAPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITEAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKMELEVYDLFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYHHDDSDTTKDFVAFRIFDGQHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFNITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGGELVYTITRPPCFSSSHRHLDAGKLFMVDSIPKPTKNPTAPGLNSFTQHAVNHMKVAYMPPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQCTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRYQHDGSEVLQDEIFLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVTITPEYIFATDADSNDSELLFLITREPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGGEIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSVPLHKSFPTYQLNITVHPVDNQPPSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGYLENTLPSAGFEKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVIINPTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLISATSKDAHNEIHNFSMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSITPVNDETPTLSKKAEVSVTVGDTRVLSSAVLSAIDKDSPREKIHYLFERLPQNGQLQLKIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDGDRFTFYLWDGDNRSPAFDCHIIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLITSPPQYGQVEYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQGVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL

Target ORF information:

RefSeq Version	XM_006238349.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238349.3

ORF Insert Sequence:

ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCTGTTGT TCTTGCTGCT CCAGCTGCTT 
ATGCTACACC TGGACCCAGC CAATGCCACC TTCATCAGCA TTAACCGGGG CCTGAGGGTG 
ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGTTTGCCAT CCCTAAAGAG 
AAGGACGCTT GCAGAGTGGA GGTGGTGATG AATGAACCCA TAACCCAGAG GGTTGGGAAA 
CTCATTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTACAATA TGTTCACAAT 
GGATGCCCAA TTCTGGACGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA 
GAGACTTTCA TGGAAACCTT CCTCTTGCGG GTCTCTCTTG TGGAACCAGA CTGTAACATC 
ATCCGCCTGA GCAGCAACGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC CCAAGCCATT 
GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACAGCCA GTCTGGACTG CACCATCAGA 
GTAGACCCCG TGAAGACTCA GCTGCCAGCC CATGGCAAGC TGGTTGTGGT GAATCGGAAG 
TCAGAGGGAC CGCGTGGAGA CCAGCCACAC AGCTTTTTCT CTGAGTCACA GCTAGGGGCA 
GAACGGAAGT GTCCGGATGG AAGCTGTACT CTGGAACCGA AGCAAGTGAC AAGTCTGAAA 
GTGAGCTGTG AGGACTTCCT GCTGACAGGG TTTCACTACC AACACATGCA GCCCCCTTCA 
CCCAACATTG ACTACATCCC CATTCAGTTG GATCTCACAG ACAGAAGGAG TAAAACCATT 
TACAAGTCAG AGAGCGCGTG GTTGCCTGTC TTCATCAGAG TTGGCATTCC TAACCAGGCC 
CCAAGGGCTG CTTTCATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ATTGACCCCT 
CTGACAACCT CCGTGCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GCTGGTGTTT 
AACATCACTG AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC 
ATCTCTTCAT TTACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCGAAC 
AACAGCCACC CTGAGAGGAG ACATTACAAG ATGGAGCTGG AGGTGTACGA CCTCTTCTTT 
GAGAGGAGCG CCCCCATCAC AGTTCACATC TCCATCAGAA CAGCAGACAC GAATGCACCT 
CGGGTGTCCT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG 
GAACAGTTTC AGATCGTGGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT 
GGTCTGCAGC ATGGACGGCT TACAGTAAGA GAGGGGAAAG GATTTCTCTT CACCGTGGCT 
GATCTTCAGG CTGGAGTTGT TCGCTATCAT CATGATGACA GTGATACCAC CAAAGACTTT 
GTGGCTTTCA GGATATTCGA TGGCCAACAC AGCAGCCATC ACAAGTTTCC TATCAACATC 
TTGCCTAAAG ATGACAGTCC CCCATTCCTC ATAACCAATG TCGTGATTGA ACTAGAAGAG 
GGGAAGACCA TCTTGATCCA AGGGTCCATG CTGAGAGCTT CAGACATGGA CTCCAGTGAT 
GACTACATCT TCTTCAATAT CACAAAGTTC CCACAGGCTG GGGAGATCAT GAAGAAGCCA 
GGGCCAAGAT TGATAGGATA TCCTGTCTCT GGCTTCCTTC AGAGAGACCT GTTCAGTGGA 
ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCTTTTGA ATTTGTCCTG 
TGGGACAGCC ATGAACCTCC AAATCTCTCA GTGCCACAGG TGGTGATGGT CCATATAACT 
CCAGTAGACG ACCAGCTTCC CAAGGAAGCT CCCGGCATAT CCCGGCATTT GATTGTCAAG 
GAAACGGAAG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAC GGAATCGCAT 
GGTGGGGAGC TTGTCTACAC AATAACACGC CCTCCATGCT TCTCCTCCAG CCACAGGCAC 
TTGGATGCTG GGAAGCTGTT CATGGTGGAC AGCATACCAA AGCCAACCAA GAATCCCACA 
GCCCCAGGGC TGAACTCATT TACTCAGCAT GCTGTGAACC ACATGAAAGT GGCCTACATG 
CCACCCATGC AGGATATAGG TCCCACACCT CGACATGTGC AGTTCACAGT GTCCGTCAGT 
AACCAGCATG GAGGTACTTT GCATGGAATC TGCTTCAACA TCACAGTGCT CCCAGTGGAC 
AATCAGGTTC CTGAGGTATT CACCAACACC CTGAGAGTGG CTGAGGGTGG CCAATGCACC 
ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CACTGGACAG CATCTGCCTC 
TCTCTTCAGG AGATGCCCCT GCATGGCGGG GTAGAGCTGG ATGGATTTCC TCTAAACCCA 
AGAGGCACAT TTTCTTGGCG AGACCTCCAT ACCTTAAAAG TTAGGTATCA ACATGATGGA 
TCTGAGGTTC TTCAGGATGA GATATTCTTG GAGGTCACAG ACGGCACAAA TTCTGCAGGA 
TTTGTTCTCT ACATTGAGGT ATTCCCTGTG AATGATGAGC CACCTGTTCT AAAGGCTGAC 
CTCATTCCCA TGATGCACTG CTCAGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT 
TTCGCTACTG ATGCAGACAG CAATGACTCA GAACTGCTAT TTCTGATTAC CCGAGAACCT 
CGGCATGGAG TAGTGAGAAA AGCTGGACTC CGTGTGGACA GGTTCTCTCA GGGAGATGTC 
ATCTCAGGAG CTGTGGCATA CAAACATACA GGTGGCGAGA TTGGCCTCGA ATCTTGTTTT 
GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGGTGAA TGGCTGTTGC 
TACAATGGAC CCGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC 
ATCACGGTGC ATCCAGTCGA CAATCAGCCA CCTTCAATTA TAATAGGTAG GACGCTCACG 
GTGGACGAAG GCTTCTCGGC AGCCCTTACG ACTCATCACT TGACTGCCAC TGACTGGGAC 
ACTGCTCCGG ATGACTTGGA ATTCATTTTG GTTTCTCATC CCCAGTTTGG CTACCTAGAA 
AACACACTGC CCTCTGCAGG CTTTGAGAAA AGGAATACGG GCATAAGCAT AGCTTCATTC 
CAGTGGAGAG ACATGAAAGC TTCGCATATT AACTATGTGC AGTCCAGGCA CCTGAGGGAG 
GAGCCAACTG CTGATGAGTT CCTGGTGTAC GCCACAGATG GGAAGCAACG CTCCTTGGAG 
ACCACATTTC ACGTTATCAT CAACCCCACA AATGATGAAG CTCCTGATTT TGTGGTACAG 
AATATCACTG TGTACGAGGG CCACATGGTA GAGCTGGATT CTGCCATCAT CAGTGCTACT 
GACAGGGATG TTCCCAAGGA CCCCTTGCTC TTCAACATCA CGCACAAGCC CAAGCATGGC 
CTCCTCCTCA TCTCGGCCAC CAGCAAGGAT GCTCATAATG AAATTCACAA CTTCTCCATG 
GAACTGCTCC AGAATGGAAT GAAGCTGGCG TATGCCCACG ATGACTCGGA GAGCTCTGCT 
GACAACTTCG TGATCCAGTT ATCAGACGGG AAACATAAAA TCCTTAAAAC CGTTTCAGTA 
AGCATCACCC CAGTGAACGA CGAGACACCA ACACTAAGCA AGAAGGCTGA AGTCTCAGTG 
ACTGTGGGCG ACACTCGTGT TCTGTCTAGT GCTGTTCTTT CAGCCATAGA TAAAGACTCA 
CCCAGGGAGA AGATTCACTA CTTATTTGAA AGACTTCCCC AAAATGGGCA ACTTCAACTT 
AAGATAGGGA GGGACTGGGT CCCTCTCTCC GCTGGCATGC AGTGCACCCA GGAGGATGTG 
GACTTGAACT TGCTAAGATA CGCGCATGCT GGTAAAATGC ATTCCCAGGA TGGAGACCGC 
TTTACCTTCT ACCTCTGGGA CGGGGACAAC AGGTCACCTG CATTTGACTG TCACATCATC 
ATTGAAGACA CAGGAAGAGG TGATATTGTC ATTCATGCGA AACCACTCGT GGTAGCCAAC 
GGTGACAGAG GTCTCTTGAC GCCTGCCACT CTTCTGGCTG AGGATGGAGC AGACAAGGCT 
GAGGAATTAC TCTACCTTAT CACTTCCCCA CCACAGTATG GCCAAGTGGA GTATGTCTAC 
TCTCCTGGAG TCCCCATTAC CAGCTTTAGC CAAATGGACA TAGCAGGACA GGCAGTCTGC 
TACATACACA AGAGCAGGAC CGCTGTCCTC AGTGACAGTT TCAGATTTAC CATCAGCAAT 
GGACTGCAGA CCCAGCAGGG GGTGTTTGAG ATCACACTGC AGGGTGTGGA CAGTGCCTTG 
CCTGTGCTGA CCAGGAACCG AAGGCTGAGG CTTGTGGAAG GGGCCATGGG CCTGCTGTCC 
ACGGATCACC TTCAGCTGAC TGACCCTGAC ACACCTCCAG AGAACCTAAC CTTCTTTCTG 
GTCCAGCTCC CGCGCCACGG GTACCTCTAC CTGAGAGGGA AAGTACTTCA GCACAACTTC 
ACCCAGCAAG ATGTAGACAG CAGGGTTGTG GCCTACCAGC ATGCAGGAGG CGGTGCCCAG 
GAGGACTGCT TTACTTTCCT GGCCACAGAC AGGAAGAACC AAGGCTTCGT TGTGGATGGG 
AAAGTTCAGA AAGAGCCCGT TCTCTTCATT ATCCAGGTGG ACCAGCTGGA TAAGAAGGCG 
CCTCGGATCA CACGCTTGCA CTCCCCTTCT CAAGTGGGGC TCTTGAAAAA TGGCTGCTAT 
GGGATTTACA TCACTTCCCA TGTGCTAAAG GCGTCTGACC CTGACACAGA GGATGACCAG 
ATCATCTTTA AGATTTTACG AGGCCCGTTG CATGGACGTC TGGAGAACAC GACAACAGGT 
GAATTCATCC ACGAGCAATT TAGCCAGAAG GACTTGAGCC GCAAGACCAT CCTTTATATC 
ATAAACCCTT CTTTGCAAGT GAATTCAGAT ATCCTGGAAT TTCAGGCCAT GGACCCCACA 
GGGAACACTG CCCCTCCCCA GAGATTGGAA CTGAAATGGT CATATATTGA ATGGTCCCAG 
ACTGAATATG AGGTCTGTGA GAATGTGGGC TTACTGACCT TGGAAGTCAC CAGAAGGGGA 
TATTCCATGG ACTCAGCCTA TGTGGACATA GAGGTTAACC AAGTGTCAGC TGTAGTTGGA 
AAAGATTTTA CTGTCACTCC ATCCAAACTG CTTCAATTTG ACCCAGGAAT GTCAACTAAG 
ATGTGGAATA TAGCAATTAC CTATGACGGA TTAGAGGAAG ACGATGAGGT CTTTGAAGTA 
ATTCTGAACT CCCCTGTGAA TGCGGTTCTT GGCACACAGA CAAAAGCAGC AGTGAAAATT 
TTGGACTCAA AAGGAGGACG GTGCCATCCT TCAAATTCCT TCAACCAAAG CAAGCACAGC 
ACGTGGGGGA AGGGACGTTG GCATCCACTG CCCTCAGGGT CATCCTCACT CACCACTTCC 
GGTTCTCCTC ATCTGGAAAG AAGGCCCCCT CCATCCATCA CCAAAGGAGA TGCTCTTCGG 
GGTTTTGATC TCACAGACCT CTCTCAAAGG AAGATGACGA CACAGGGGAA TGGCAAATCA 
GTCCTCCCAT CCTCTGTTTG TAGAAATGGA ACAGACACCA TCTACAATTA TCATGGTATA 
GTTTCCTTGA AACTGGAGGG GGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC 
GTCATTCGTC AGCCACAGAG GACAGTCAAG GTGGCAGAGC TGCCTCTAGC CGATAAGGTA 
GAACCCACAA CCGACTCGAA CTTTCTCAGT CAGGGTCTAC GGCCCTTGTT TCCAAAGAAC 
TGTTCTGTGG AATTAAAGGG ACTCTTTCAT TTGGAAGAAC ACACCCACAG ATTGTATCAA 
TGTGATGGGA TCGCCTGGAA CGTCTGGAGC TCCCAAACCA AGGGGCTGGA GGACAGATCC 
TGCCCAGTTG GATGGCTTCT TCATTCCGAC TACTGTCACG TCTTGGTCAC AAGGCAGAAA 
GGCACCTGGG CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCAC CCTTGTGACC 
GTATTCTCCA GGAGGCACAT GCAGTGGCTG TGGGCTATCA GCGGAAGAAA GCCCTTCTGG 
ATAGGCTTGA AGAACCAAGT GCGAACTGGC CACTGGGAGT GGATTGGAGG TGAACCTGTT 
GCTTTCACCA ACTGGAGGAG AGGGCCCCCT CTGCACCCGA AGCCTGGAAA GAACTGTGTT 
TTGGTTCAAA GAAGAGGACA ATGGCAAACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT 
TTTGTGTGCT CAAGGAAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem1 ( XM_008763770.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761992.1 Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X2, mRNA.

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CloneID	ORa45500
Clone ID Related Accession (Same CDS sequence)	XM_008763770.2
Accession Version	XM_008763770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCT TCCTCTTGCG GGTCTCTCTT GTGGAACCAG ACTGTAACAT CATCCGCCTG 
AGCAGCAACG TGTTGGAAGT GACTGAATTC TATGGCTTAT CCCAAGCCAT TGATAAGAAC 
CTGCTCCAGT TTGATTATGA TAGGACAGCC AGTCTGGACT GCACCATCAG AGTAGACCCC 
GTGAAGACTC AGCTGCCAGC CCATGGCAAG CTGGTTGTGG TGAATCGGAA GTCAGAGGGA 
CCGCGTGGAG ACCAGCCACA CAGCTTTTTC TCTGAGTCAC AGCTAGGGGC AGAACGGAAG 
TGTCCGGATG GAAGCTGTAC TCTGGAACCG AAGCAAGTGA CAAGTCTGAA AGTGAGCTGT 
GAGGACTTCC TGCTGACAGG GTTTCACTAC CAACACATGC AGCCCCCTTC ACCCAACATT 
GACTACATCC CCATTCAGTT GGATCTCACA GACAGAAGGA GTAAAACCAT TTACAAGTCA 
GAGAGCGCGT GGTTGCCTGT CTTCATCAGA GTTGGCATTC CTAACCAGGC CCCAAGGGCT 
GCTTTCATGG CCATGTTTGT TCTGGAGGTG GACCAGTTCA TATTGACCCC TCTGACAACC 
TCCGTGCTGG ACTGTGAGGA GGATGAGACT CCGAAACCCT TGCTGGTGTT TAACATCACT 
GAAGCCCCAC TCCAGGGCTA TGTGACCCAC CTGCTGGATC ATACCAGACC CATCTCTTCA 
TTTACCTGGA AGGATCTCAG TGACATGCAA GTTGCCTACC AGCCACCGAA CAACAGCCAC 
CCTGAGAGGA GACATTACAA GATGGAGCTG GAGGTGTACG ACCTCTTCTT TGAGAGGAGC 
GCCCCCATCA CAGTTCACAT CTCCATCAGA ACAGCAGACA CGAATGCACC TCGGGTGTCC 
TGGAATACAG GTTTGAATCT CCTAGAGGGG CAGTCTCGGG CCATCACTTG GGAACAGTTT 
CAGATCGTGG ACAATGATGA CATTGGTGCT GTGCAGTTGG TCACCATTGG TGGTCTGCAG 
CATGGACGGC TTACAGTAAG AGAGGGGAAA GGATTTCTCT TCACCGTGGC TGATCTTCAG 
GCTGGAGTTG TTCGCTATCA TCATGATGAC AGTGATACCA CCAAAGACTT TGTGGCTTTC 
AGGATATTCG ATGGCCAACA CAGCAGCCAT CACAAGTTTC CTATCAACAT CTTGCCTAAA 
GATGACAGTC CCCCATTCCT CATAACCAAT GTCGTGATTG AACTAGAAGA GGGGAAGACC 
ATCTTGATCC AAGGGTCCAT GCTGAGAGCT TCAGACATGG ACTCCAGTGA TGACTACATC 
TTCTTCAATA TCACAAAGTT CCCACAGGCT GGGGAGATCA TGAAGAAGCC AGGGCCAAGA 
TTGATAGGAT ATCCTGTCTC TGGCTTCCTT CAGAGAGACC TGTTCAGTGG AATCATTTAC 
TATCGTCACT TTGGTGGAGA AATCTTTGAA GATTCTTTTG AATTTGTCCT GTGGGACAGC 
CATGAACCTC CAAATCTCTC AGTGCCACAG GTGGTGATGG TCCATATAAC TCCAGTAGAC 
GACCAGCTTC CCAAGGAAGC TCCCGGCATA TCCCGGCATT TGATTGTCAA GGAAACGGAA 
GTGGCCTACA TCACTAAAAA GCACCTACAT TTCCTAGACA CGGAATCGCA TGGTGGGGAG 
CTTGTCTACA CAATAACACG CCCTCCATGC TTCTCCTCCA GCCACAGGCA CTTGGATGCT 
GGGAAGCTGT TCATGGTGGA CAGCATACCA AAGCCAACCA AGAATCCCAC AGCCCCAGGG 
CTGAACTCAT TTACTCAGCA TGCTGTGAAC CACATGAAAG TGGCCTACAT GCCACCCATG 
CAGGATATAG GTCCCACACC TCGACATGTG CAGTTCACAG TGTCCGTCAG TAACCAGCAT 
GGAGGTACTT TGCATGGAAT CTGCTTCAAC ATCACAGTGC TCCCAGTGGA CAATCAGGTT 
CCTGAGGTAT TCACCAACAC CCTGAGAGTG GCTGAGGGTG GCCAATGCAC CATCAGCACA 
GAGCACATTT TGGTTTCTGA TGTGGACACC CCACTGGACA GCATCTGCCT CTCTCTTCAG 
GAGATGCCCC TGCATGGCGG GGTAGAGCTG GATGGATTTC CTCTAAACCC AAGAGGCACA 
TTTTCTTGGC GAGACCTCCA TACCTTAAAA GTTAGGTATC AACATGATGG ATCTGAGGTT 
CTTCAGGATG AGATATTCTT GGAGGTCACA GACGGCACAA ATTCTGCAGG ATTTGTTCTC 
TACATTGAGG TATTCCCTGT GAATGATGAG CCACCTGTTC TAAAGGCTGA CCTCATTCCC 
ATGATGCACT GCTCAGAGGG TGGAGAGGTG ACCATCACCC CTGAATACAT TTTCGCTACT 
GATGCAGACA GCAATGACTC AGAACTGCTA TTTCTGATTA CCCGAGAACC TCGGCATGGA 
GTAGTGAGAA AAGCTGGACT CCGTGTGGAC AGGTTCTCTC AGGGAGATGT CATCTCAGGA 
GCTGTGGCAT ACAAACATAC AGGTGGCGAG ATTGGCCTCG AATCTTGTTT TGACACTGTT 
GTATTGGTGG TTTCGGATGG TGAAGCTGAC CCTTTGGTGA ATGGCTGTTG CTACAATGGA 
CCCGATTCAT CTGTTCCTCT TCATAAGTCC TTCCCCACAT ACCAGCTCAA CATCACGGTG 
CATCCAGTCG ACAATCAGCC ACCTTCAATT ATAATAGGTA GGACGCTCAC GGTGGACGAA 
GGCTTCTCGG CAGCCCTTAC GACTCATCAC TTGACTGCCA CTGACTGGGA CACTGCTCCG 
GATGACTTGG AATTCATTTT GGTTTCTCAT CCCCAGTTTG GCTACCTAGA AAACACACTG 
CCCTCTGCAG GCTTTGAGAA AAGGAATACG GGCATAAGCA TAGCTTCATT CCAGTGGAGA 
GACATGAAAG CTTCGCATAT TAACTATGTG CAGTCCAGGC ACCTGAGGGA GGAGCCAACT 
GCTGATGAGT TCCTGGTGTA CGCCACAGAT GGGAAGCAAC GCTCCTTGGA GACCACATTT 
CACGTTATCA TCAACCCCAC AAATGATGAA GCTCCTGATT TTGTGGTACA GAATATCACT 
GTGTACGAGG GCCACATGGT AGAGCTGGAT TCTGCCATCA TCAGTGCTAC TGACAGGGAT 
GTTCCCAAGG ACCCCTTGCT CTTCAACATC ACGCACAAGC CCAAGCATGG CCTCCTCCTC 
ATCTCGGCCA CCAGCAAGGA TGCTCATAAT GAAATTCACA ACTTCTCCAT GGAACTGCTC 
CAGAATGGAA TGAAGCTGGC GTATGCCCAC GATGACTCGG AGAGCTCTGC TGACAACTTC 
GTGATCCAGT TATCAGACGG GAAACATAAA ATCCTTAAAA CCGTTTCAGT AAGCATCACC 
CCAGTGAACG ACGAGACACC AACACTAAGC AAGAAGGCTG AAGTCTCAGT GACTGTGGGC 
GACACTCGTG TTCTGTCTAG TGCTGTTCTT TCAGCCATAG ATAAAGACTC ACCCAGGGAG 
AAGATTCACT ACTTATTTGA AAGACTTCCC CAAAATGGGC AACTTCAACT TAAGATAGGG 
AGGGACTGGG TCCCTCTCTC CGCTGGCATG CAGTGCACCC AGGAGGATGT GGACTTGAAC 
TTGCTAAGAT ACGCGCATGC TGGTAAAATG CATTCCCAGG ATGGAGACCG CTTTACCTTC 
TACCTCTGGG ACGGGGACAA CAGGTCACCT GCATTTGACT GTCACATCAT CATTGAAGAC 
ACAGGAAGAG GTGATATTGT CATTCATGCG AAACCACTCG TGGTAGCCAA CGGTGACAGA 
GGTCTCTTGA CGCCTGCCAC TCTTCTGGCT GAGGATGGAG CAGACAAGGC TGAGGAATTA 
CTCTACCTTA TCACTTCCCC ACCACAGTAT GGCCAAGTGG AGTATGTCTA CTCTCCTGGA 
GTCCCCATTA CCAGCTTTAG CCAAATGGAC ATAGCAGGAC AGGCAGTCTG CTACATACAC 
AAGAGCAGGA CCGCTGTCCT CAGTGACAGT TTCAGATTTA CCATCAGCAA TGGACTGCAG 
ACCCAGCAGG GGGTGTTTGA GATCACACTG CAGGGTGTGG ACAGTGCCTT GCCTGTGCTG 
ACCAGGAACC GAAGGCTGAG GCTTGTGGAA GGGGCCATGG GCCTGCTGTC CACGGATCAC 
CTTCAGCTGA CTGACCCTGA CACACCTCCA GAGAACCTAA CCTTCTTTCT GGTCCAGCTC 
CCGCGCCACG GGTACCTCTA CCTGAGAGGG AAAGTACTTC AGCACAACTT CACCCAGCAA 
GATGTAGACA GCAGGGTTGT GGCCTACCAG CATGCAGGAG GCGGTGCCCA GGAGGACTGC 
TTTACTTTCC TGGCCACAGA CAGGAAGAAC CAAGGCTTCG TTGTGGATGG GAAAGTTCAG 
AAAGAGCCCG TTCTCTTCAT TATCCAGGTG GACCAGCTGG ATAAGAAGGC GCCTCGGATC 
ACACGCTTGC ACTCCCCTTC TCAAGTGGGG CTCTTGAAAA ATGGCTGCTA TGGGATTTAC 
ATCACTTCCC ATGTGCTAAA GGCGTCTGAC CCTGACACAG AGGATGACCA GATCATCTTT 
AAGATTTTAC GAGGCCCGTT GCATGGACGT CTGGAGAACA CGACAACAGG TGAATTCATC 
CACGAGCAAT TTAGCCAGAA GGACTTGAGC CGCAAGACCA TCCTTTATAT CATAAACCCT 
TCTTTGCAAG TGAATTCAGA TATCCTGGAA TTTCAGGCCA TGGACCCCAC AGGGAACACT 
GCCCCTCCCC AGAGATTGGA ACTGAAATGG TCATATATTG AATGGTCCCA GACTGAATAT 
GAGGTCTGTG AGAATGTGGG CTTACTGACC TTGGAAGTCA CCAGAAGGGG ATATTCCATG 
GACTCAGCCT ATGTGGACAT AGAGGTTAAC CAAGTGTCAG CTGTAGTTGG AAAAGATTTT 
ACTGTCACTC CATCCAAACT GCTTCAATTT GACCCAGGAA TGTCAACTAA GATGTGGAAT 
ATAGCAATTA CCTATGACGG ATTAGAGGAA GACGATGAGG TCTTTGAAGT AATTCTGAAC 
TCCCCTGTGA ATGCGGTTCT TGGCACACAG ACAAAAGCAG CAGTGAAAAT TTTGGACTCA 
AAAGGAGGAC GGTGCCATCC TTCAAATTCC TTCAACCAAA GCAAGCACAG CACGTGGGGG 
AAGGGACGTT GGCATCCACT GCCCTCAGGG TCATCCTCAC TCACCACTTC CGGTTCTCCT 
CATCTGGAAA GAAGGCCCCC TCCATCCATC ACCAAAGGAG ATGCTCTTCG GGGTTTTGAT 
CTCACAGACC TCTCTCAAAG GAAGATGACG ACACAGGGGA ATGGCAAATC AGTCCTCCCA 
TCCTCTGTTT GTAGAAATGG AACAGACACC ATCTACAATT ATCATGGTAT AGTTTCCTTG 
AAACTGGAGG GGGACAGATT CTCAGCTCAC AAACGGAAGG CCAAAATATC CGTCATTCGT 
CAGCCACAGA GGACAGTCAA GGTGGCAGAG CTGCCTCTAG CCGATAAGGT AGAACCCACA 
ACCGACTCGA ACTTTCTCAG TCAGGGTCTA CGGCCCTTGT TTCCAAAGAA CTGTTCTGTG 
GAATTAAAGG GACTCTTTCA TTTGGAAGAA CACACCCACA GATTGTATCA ATGTGATGGG 
ATCGCCTGGA ACGTCTGGAG CTCCCAAACC AAGGGGCTGG AGGACAGATC CTGCCCAGTT 
GGATGGCTTC TTCATTCCGA CTACTGTCAC GTCTTGGTCA CAAGGCAGAA AGGCACCTGG 
GCCACAGCTA CCCGAGCTTG CAGAGAACAG CACCAGGGCA CCCTTGTGAC CGTATTCTCC 
AGGAGGCACA TGCAGTGGCT GTGGGCTATC AGCGGAAGAA AGCCCTTCTG GATAGGCTTG 
AAGAACCAAG TGCGAACTGG CCACTGGGAG TGGATTGGAG GTGAACCTGT TGCTTTCACC 
AACTGGAGGA GAGGGCCCCC TCTGCACCCG AAGCCTGGAA AGAACTGTGT TTTGGTTCAA 
AGAAGAGGAC AATGGCAAAC AAAGAACTGT AGCAAAGGCA AGGCTCACAA TTTTGTGTGC 
TCAAGGAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761992.1
CDS	576..6710
Translation	METFLLRVSLVEPDCNIIRLSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRVDPVKTQLPAHGKLVVVNRKSEGPRGDQPHSFFSESQLGAERKCPDGSCTLEPKQVTSLKVSCEDFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTIYKSESAWLPVFIRVGIPNQAPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITEAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKMELEVYDLFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYHHDDSDTTKDFVAFRIFDGQHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFNITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGGELVYTITRPPCFSSSHRHLDAGKLFMVDSIPKPTKNPTAPGLNSFTQHAVNHMKVAYMPPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQCTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRYQHDGSEVLQDEIFLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVTITPEYIFATDADSNDSELLFLITREPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGGEIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSVPLHKSFPTYQLNITVHPVDNQPPSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGYLENTLPSAGFEKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVIINPTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLISATSKDAHNEIHNFSMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSITPVNDETPTLSKKAEVSVTVGDTRVLSSAVLSAIDKDSPREKIHYLFERLPQNGQLQLKIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDGDRFTFYLWDGDNRSPAFDCHIIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLITSPPQYGQVEYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQGVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL

Target ORF information:

RefSeq Version	XM_008763770.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763770.2

ORF Insert Sequence:

ATGGAAACCT TCCTCTTGCG GGTCTCTCTT GTGGAACCAG ACTGTAACAT CATCCGCCTG 
AGCAGCAACG TGTTGGAAGT GACTGAATTC TATGGCTTAT CCCAAGCCAT TGATAAGAAC 
CTGCTCCAGT TTGATTATGA TAGGACAGCC AGTCTGGACT GCACCATCAG AGTAGACCCC 
GTGAAGACTC AGCTGCCAGC CCATGGCAAG CTGGTTGTGG TGAATCGGAA GTCAGAGGGA 
CCGCGTGGAG ACCAGCCACA CAGCTTTTTC TCTGAGTCAC AGCTAGGGGC AGAACGGAAG 
TGTCCGGATG GAAGCTGTAC TCTGGAACCG AAGCAAGTGA CAAGTCTGAA AGTGAGCTGT 
GAGGACTTCC TGCTGACAGG GTTTCACTAC CAACACATGC AGCCCCCTTC ACCCAACATT 
GACTACATCC CCATTCAGTT GGATCTCACA GACAGAAGGA GTAAAACCAT TTACAAGTCA 
GAGAGCGCGT GGTTGCCTGT CTTCATCAGA GTTGGCATTC CTAACCAGGC CCCAAGGGCT 
GCTTTCATGG CCATGTTTGT TCTGGAGGTG GACCAGTTCA TATTGACCCC TCTGACAACC 
TCCGTGCTGG ACTGTGAGGA GGATGAGACT CCGAAACCCT TGCTGGTGTT TAACATCACT 
GAAGCCCCAC TCCAGGGCTA TGTGACCCAC CTGCTGGATC ATACCAGACC CATCTCTTCA 
TTTACCTGGA AGGATCTCAG TGACATGCAA GTTGCCTACC AGCCACCGAA CAACAGCCAC 
CCTGAGAGGA GACATTACAA GATGGAGCTG GAGGTGTACG ACCTCTTCTT TGAGAGGAGC 
GCCCCCATCA CAGTTCACAT CTCCATCAGA ACAGCAGACA CGAATGCACC TCGGGTGTCC 
TGGAATACAG GTTTGAATCT CCTAGAGGGG CAGTCTCGGG CCATCACTTG GGAACAGTTT 
CAGATCGTGG ACAATGATGA CATTGGTGCT GTGCAGTTGG TCACCATTGG TGGTCTGCAG 
CATGGACGGC TTACAGTAAG AGAGGGGAAA GGATTTCTCT TCACCGTGGC TGATCTTCAG 
GCTGGAGTTG TTCGCTATCA TCATGATGAC AGTGATACCA CCAAAGACTT TGTGGCTTTC 
AGGATATTCG ATGGCCAACA CAGCAGCCAT CACAAGTTTC CTATCAACAT CTTGCCTAAA 
GATGACAGTC CCCCATTCCT CATAACCAAT GTCGTGATTG AACTAGAAGA GGGGAAGACC 
ATCTTGATCC AAGGGTCCAT GCTGAGAGCT TCAGACATGG ACTCCAGTGA TGACTACATC 
TTCTTCAATA TCACAAAGTT CCCACAGGCT GGGGAGATCA TGAAGAAGCC AGGGCCAAGA 
TTGATAGGAT ATCCTGTCTC TGGCTTCCTT CAGAGAGACC TGTTCAGTGG AATCATTTAC 
TATCGTCACT TTGGTGGAGA AATCTTTGAA GATTCTTTTG AATTTGTCCT GTGGGACAGC 
CATGAACCTC CAAATCTCTC AGTGCCACAG GTGGTGATGG TCCATATAAC TCCAGTAGAC 
GACCAGCTTC CCAAGGAAGC TCCCGGCATA TCCCGGCATT TGATTGTCAA GGAAACGGAA 
GTGGCCTACA TCACTAAAAA GCACCTACAT TTCCTAGACA CGGAATCGCA TGGTGGGGAG 
CTTGTCTACA CAATAACACG CCCTCCATGC TTCTCCTCCA GCCACAGGCA CTTGGATGCT 
GGGAAGCTGT TCATGGTGGA CAGCATACCA AAGCCAACCA AGAATCCCAC AGCCCCAGGG 
CTGAACTCAT TTACTCAGCA TGCTGTGAAC CACATGAAAG TGGCCTACAT GCCACCCATG 
CAGGATATAG GTCCCACACC TCGACATGTG CAGTTCACAG TGTCCGTCAG TAACCAGCAT 
GGAGGTACTT TGCATGGAAT CTGCTTCAAC ATCACAGTGC TCCCAGTGGA CAATCAGGTT 
CCTGAGGTAT TCACCAACAC CCTGAGAGTG GCTGAGGGTG GCCAATGCAC CATCAGCACA 
GAGCACATTT TGGTTTCTGA TGTGGACACC CCACTGGACA GCATCTGCCT CTCTCTTCAG 
GAGATGCCCC TGCATGGCGG GGTAGAGCTG GATGGATTTC CTCTAAACCC AAGAGGCACA 
TTTTCTTGGC GAGACCTCCA TACCTTAAAA GTTAGGTATC AACATGATGG ATCTGAGGTT 
CTTCAGGATG AGATATTCTT GGAGGTCACA GACGGCACAA ATTCTGCAGG ATTTGTTCTC 
TACATTGAGG TATTCCCTGT GAATGATGAG CCACCTGTTC TAAAGGCTGA CCTCATTCCC 
ATGATGCACT GCTCAGAGGG TGGAGAGGTG ACCATCACCC CTGAATACAT TTTCGCTACT 
GATGCAGACA GCAATGACTC AGAACTGCTA TTTCTGATTA CCCGAGAACC TCGGCATGGA 
GTAGTGAGAA AAGCTGGACT CCGTGTGGAC AGGTTCTCTC AGGGAGATGT CATCTCAGGA 
GCTGTGGCAT ACAAACATAC AGGTGGCGAG ATTGGCCTCG AATCTTGTTT TGACACTGTT 
GTATTGGTGG TTTCGGATGG TGAAGCTGAC CCTTTGGTGA ATGGCTGTTG CTACAATGGA 
CCCGATTCAT CTGTTCCTCT TCATAAGTCC TTCCCCACAT ACCAGCTCAA CATCACGGTG 
CATCCAGTCG ACAATCAGCC ACCTTCAATT ATAATAGGTA GGACGCTCAC GGTGGACGAA 
GGCTTCTCGG CAGCCCTTAC GACTCATCAC TTGACTGCCA CTGACTGGGA CACTGCTCCG 
GATGACTTGG AATTCATTTT GGTTTCTCAT CCCCAGTTTG GCTACCTAGA AAACACACTG 
CCCTCTGCAG GCTTTGAGAA AAGGAATACG GGCATAAGCA TAGCTTCATT CCAGTGGAGA 
GACATGAAAG CTTCGCATAT TAACTATGTG CAGTCCAGGC ACCTGAGGGA GGAGCCAACT 
GCTGATGAGT TCCTGGTGTA CGCCACAGAT GGGAAGCAAC GCTCCTTGGA GACCACATTT 
CACGTTATCA TCAACCCCAC AAATGATGAA GCTCCTGATT TTGTGGTACA GAATATCACT 
GTGTACGAGG GCCACATGGT AGAGCTGGAT TCTGCCATCA TCAGTGCTAC TGACAGGGAT 
GTTCCCAAGG ACCCCTTGCT CTTCAACATC ACGCACAAGC CCAAGCATGG CCTCCTCCTC 
ATCTCGGCCA CCAGCAAGGA TGCTCATAAT GAAATTCACA ACTTCTCCAT GGAACTGCTC 
CAGAATGGAA TGAAGCTGGC GTATGCCCAC GATGACTCGG AGAGCTCTGC TGACAACTTC 
GTGATCCAGT TATCAGACGG GAAACATAAA ATCCTTAAAA CCGTTTCAGT AAGCATCACC 
CCAGTGAACG ACGAGACACC AACACTAAGC AAGAAGGCTG AAGTCTCAGT GACTGTGGGC 
GACACTCGTG TTCTGTCTAG TGCTGTTCTT TCAGCCATAG ATAAAGACTC ACCCAGGGAG 
AAGATTCACT ACTTATTTGA AAGACTTCCC CAAAATGGGC AACTTCAACT TAAGATAGGG 
AGGGACTGGG TCCCTCTCTC CGCTGGCATG CAGTGCACCC AGGAGGATGT GGACTTGAAC 
TTGCTAAGAT ACGCGCATGC TGGTAAAATG CATTCCCAGG ATGGAGACCG CTTTACCTTC 
TACCTCTGGG ACGGGGACAA CAGGTCACCT GCATTTGACT GTCACATCAT CATTGAAGAC 
ACAGGAAGAG GTGATATTGT CATTCATGCG AAACCACTCG TGGTAGCCAA CGGTGACAGA 
GGTCTCTTGA CGCCTGCCAC TCTTCTGGCT GAGGATGGAG CAGACAAGGC TGAGGAATTA 
CTCTACCTTA TCACTTCCCC ACCACAGTAT GGCCAAGTGG AGTATGTCTA CTCTCCTGGA 
GTCCCCATTA CCAGCTTTAG CCAAATGGAC ATAGCAGGAC AGGCAGTCTG CTACATACAC 
AAGAGCAGGA CCGCTGTCCT CAGTGACAGT TTCAGATTTA CCATCAGCAA TGGACTGCAG 
ACCCAGCAGG GGGTGTTTGA GATCACACTG CAGGGTGTGG ACAGTGCCTT GCCTGTGCTG 
ACCAGGAACC GAAGGCTGAG GCTTGTGGAA GGGGCCATGG GCCTGCTGTC CACGGATCAC 
CTTCAGCTGA CTGACCCTGA CACACCTCCA GAGAACCTAA CCTTCTTTCT GGTCCAGCTC 
CCGCGCCACG GGTACCTCTA CCTGAGAGGG AAAGTACTTC AGCACAACTT CACCCAGCAA 
GATGTAGACA GCAGGGTTGT GGCCTACCAG CATGCAGGAG GCGGTGCCCA GGAGGACTGC 
TTTACTTTCC TGGCCACAGA CAGGAAGAAC CAAGGCTTCG TTGTGGATGG GAAAGTTCAG 
AAAGAGCCCG TTCTCTTCAT TATCCAGGTG GACCAGCTGG ATAAGAAGGC GCCTCGGATC 
ACACGCTTGC ACTCCCCTTC TCAAGTGGGG CTCTTGAAAA ATGGCTGCTA TGGGATTTAC 
ATCACTTCCC ATGTGCTAAA GGCGTCTGAC CCTGACACAG AGGATGACCA GATCATCTTT 
AAGATTTTAC GAGGCCCGTT GCATGGACGT CTGGAGAACA CGACAACAGG TGAATTCATC 
CACGAGCAAT TTAGCCAGAA GGACTTGAGC CGCAAGACCA TCCTTTATAT CATAAACCCT 
TCTTTGCAAG TGAATTCAGA TATCCTGGAA TTTCAGGCCA TGGACCCCAC AGGGAACACT 
GCCCCTCCCC AGAGATTGGA ACTGAAATGG TCATATATTG AATGGTCCCA GACTGAATAT 
GAGGTCTGTG AGAATGTGGG CTTACTGACC TTGGAAGTCA CCAGAAGGGG ATATTCCATG 
GACTCAGCCT ATGTGGACAT AGAGGTTAAC CAAGTGTCAG CTGTAGTTGG AAAAGATTTT 
ACTGTCACTC CATCCAAACT GCTTCAATTT GACCCAGGAA TGTCAACTAA GATGTGGAAT 
ATAGCAATTA CCTATGACGG ATTAGAGGAA GACGATGAGG TCTTTGAAGT AATTCTGAAC 
TCCCCTGTGA ATGCGGTTCT TGGCACACAG ACAAAAGCAG CAGTGAAAAT TTTGGACTCA 
AAAGGAGGAC GGTGCCATCC TTCAAATTCC TTCAACCAAA GCAAGCACAG CACGTGGGGG 
AAGGGACGTT GGCATCCACT GCCCTCAGGG TCATCCTCAC TCACCACTTC CGGTTCTCCT 
CATCTGGAAA GAAGGCCCCC TCCATCCATC ACCAAAGGAG ATGCTCTTCG GGGTTTTGAT 
CTCACAGACC TCTCTCAAAG GAAGATGACG ACACAGGGGA ATGGCAAATC AGTCCTCCCA 
TCCTCTGTTT GTAGAAATGG AACAGACACC ATCTACAATT ATCATGGTAT AGTTTCCTTG 
AAACTGGAGG GGGACAGATT CTCAGCTCAC AAACGGAAGG CCAAAATATC CGTCATTCGT 
CAGCCACAGA GGACAGTCAA GGTGGCAGAG CTGCCTCTAG CCGATAAGGT AGAACCCACA 
ACCGACTCGA ACTTTCTCAG TCAGGGTCTA CGGCCCTTGT TTCCAAAGAA CTGTTCTGTG 
GAATTAAAGG GACTCTTTCA TTTGGAAGAA CACACCCACA GATTGTATCA ATGTGATGGG 
ATCGCCTGGA ACGTCTGGAG CTCCCAAACC AAGGGGCTGG AGGACAGATC CTGCCCAGTT 
GGATGGCTTC TTCATTCCGA CTACTGTCAC GTCTTGGTCA CAAGGCAGAA AGGCACCTGG 
GCCACAGCTA CCCGAGCTTG CAGAGAACAG CACCAGGGCA CCCTTGTGAC CGTATTCTCC 
AGGAGGCACA TGCAGTGGCT GTGGGCTATC AGCGGAAGAA AGCCCTTCTG GATAGGCTTG 
AAGAACCAAG TGCGAACTGG CCACTGGGAG TGGATTGGAG GTGAACCTGT TGCTTTCACC 
AACTGGAGGA GAGGGCCCCC TCTGCACCCG AAGCCTGGAA AGAACTGTGT TTTGGTTCAA 
AGAAGAGGAC AATGGCAAAC AAAGAACTGT AGCAAAGGCA AGGCTCACAA TTTTGTGTGC 
TCAAGGAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Expression of FRAS1-Related Extracellular Matrix 1 Is Decreased in Nitrofen-Induced Congenital Diaphragmatic Hernia.
European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie26(1)81-5(2016 Feb)
Takahashi T,Friedmacher F,Puri P

Deficiency of FRAS1-related extracellular matrix 1 (FREM1) causes congenital diaphragmatic hernia in humans and mice.
Human molecular genetics22(5)1026-38(2013 Mar)
Beck TF,Veenma D,Shchelochkov OA,Yu Z,Kim BJ,Zaveri HP,van Bever Y,Choi S,Douben H,Bertin TK,Patel PI,Lee B,Tibboel D,de Klein A,Stockton DW,Justice MJ,Scott DA

Novel frem1-related mouse phenotypes and evidence of genetic interactions with gata4 and slit3.
PloS one8(3)e58830(2013)
Beck TF,Shchelochkov OA,Yu Z,Kim BJ,Hern?ndez-Garc?a A,Zaveri HP,Bishop C,Overbeek PA,Stockton DW,Justice MJ,Scott DA

Heterozygous mutations of FREM1 are associated with an increased risk of isolated metopic craniosynostosis in humans and mice.
PLoS genetics7(9)e1002278(2011 Sep)
Vissers LE,Cox TC,Maga AM,Short KM,Wiradjaja F,Janssen IM,Jehee F,Bertola D,Liu J,Yagnik G,Sekiguchi K,Kiyozumi D,van Bokhoven H,Marcelis C,Cunningham ML,Anderson PJ,Boyadjiev SA,Passos-Bueno MR,Veltman JA,Smyth I,Buckley MF,Roscioli T

Manitoba-oculo-tricho-anal (MOTA) syndrome is caused by mutations in FREM1.
Journal of medical genetics48(6)375-82(2011 Jun)
Slavotinek AM,Baranzini SE,Schanze D,Labelle-Dumais C,Short KM,Chao R,Yahyavi M,Bijlsma EK,Chu C,Musone S,Wheatley A,Kwok PY,Marles S,Fryns JP,Maga AM,Hassan MG,Gould DB,Madireddy L,Li C,Cox TC,Smyth I,Chudley AE,Zenker M

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Frem1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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