Ppp3r1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp3r1
Entrez Gene ID	29748
Full Name	protein phosphatase 3, regulatory subunit B, alpha
General protein information	Preferred Names protein phosphatase 3, regulatory subunit B, alpha Names calcineurin subunit B type 1 protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) protein phospatase 3, regulatory subunit B, alpha isoform,type 1 protein phosphatase 2B regulatory subunit 1 protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	regulatory subunit of the enzyme that catalyzes the calcium-dependent removal of serine- and threonine-bound phosphate groups [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251517.3	XP_006251579.3	calcineurin subunit B type 1 isoform X1
NM_017309.2	NP_059005.1	calcineurin subunit B type 1
XM_017599150.1	XP_017454639.1	calcineurin subunit B type 1 isoform X2
XM_008770426.2	XP_008768648.1	calcineurin subunit B type 1 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3R1	XP_855383.3
Magnaporthe oryzae (rice blast fungus)	MGG_06933	XP_003709672.1
Neurospora crassa	NCU03833	XP_960592.2
Pan troglodytes (chimpanzee)	PPP3R1	XP_003309091.1
Mus musculus (house mouse)	Ppp3r1	NP_077779.2
Rattus norvegicus (Norway rat)	Ppp3r1	NP_059005.1
Eremothecium gossypii	AGOS_AER096C	NP_984956.1
Schizosaccharomyces pombe (fission yeast)	SPCC830.06	NP_588476.1
	LOC100428682	XP_002799342.1
Bos taurus (cattle)	PPP3R1	NP_777008.1
Gallus gallus (chicken)	PPP3R1	NP_989707.1
Drosophila melanogaster (fruit fly)	CanB	NP_524741.1
Arabidopsis thaliana (thale cress)	AT3G18430	NP_001189924.1
Oryza sativa (rice)	Os08g0442300	NP_001061916.2
Saccharomyces cerevisiae (baker's yeast)	CNB1	NP_012731.1
Xenopus tropicalis (tropical clawed frog)	ppp3r1	NP_989400.1
Homo sapiens (human)	PPP3R1	NP_000936.1
Danio rerio (zebrafish)	ppp3r1a	XP_692770.2
Drosophila melanogaster (fruit fly)	CanB2	NP_524874.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004298	XP_313573.5
Caenorhabditis elegans (roundworm)	cnb-1	NP_001256317.1
Kluyveromyces lactis	KLLA0D02992g	XP_453201.1

Gene Ontology
Bibliography

Related articles in PubMed

Investigating the human Calcineurin Interaction Network using the ??LxVP SLiM.
Sheftic SR, Page R, Peti W
Scientific reports638920(2016 Dec)

Cooperative autoinhibition and multi-level activation mechanisms of calcineurin.
Li SJ, Wang J, Ma L, Lu C, Wang J, Wu JW, Wang ZX
Cell research26(3)336-49(2016 Mar)

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Gr?nborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, Jahn O, Brose N, Herzog E
The EMBO journal33(2)157-70(2014 Jan)

Structural basis of calcineurin activation by calmodulin.
Ye Q, Feng Y, Yin Y, Faucher F, Currie MA, Rahman MN, Jin J, Li S, Wei Q, Jia Z
Cellular signalling25(12)2661-7(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study proposes that the structural basis of calcineurin activation by calmodulin is through displacement of the disordered fragment of the autoinhibitory domain which otherwise impedes active site access.
Title: Structural basis of calcineurin activation by calmodulin.

the calcineurin regulatory subunit, CANB1, and its catalytic binding partner PP2BA, were decreased in the fluid percussion model of traumatic brain injury in rats
Title: Detection of structural and metabolic changes in traumatically injured hippocampus by quantitative differential proteomics.

These findings reveal a novel function for CnB protein in cardiac stress response and suggest a possible application of CnB in coronary disease therapy.
Title: Calcineurin B subunit acts as a potential agent for preventing cardiac ischemia/reperfusion injury.

CaN content was affected by exposure to ammonia and glutamine; the serum content of the culture medium had a strong influence on the astroglial response to ammonium chloride, and glutamine exposure only reproduced some of the ammonia effects.
Title: Effect of ammonia, glutamine, and serum on calcineurin, p38MAPK-diP, GADD153/CHOP10, and CNTF in primary rat astrocyte cultures.

The enzymatic properties of CaN heterodimers comprised of the regulatory B subunit (CnB) with either the alpha or beta catalytic subunit were compared using in vitro phosphatase assays.
Title: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the alpha or beta catalytic subunit.

The following Ppp3r1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp3r1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13485	NM_017309.2 Latest version!	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa43379	XM_008770426.2 Latest version!	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa58271	XM_006251517.3 Latest version!	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa58272	XM_017599150.1 Latest version!	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp3r1 ( NM_017309.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059005.1 Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), mRNA.

Price & Availability↑

CloneID	ORa13485
Clone ID Related Accession (Same CDS sequence)	NM_017309.2
Accession Version	NM_017309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	calcineurin subunit B type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088855.1. On Jan 12, 2005 this sequence version replaced NM_017309.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088855.1, FQ212066.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAATG AGGCGAGTTA CCCTTTGGAA ATGTGCTCAC ACTTCGATGC TGATGAGATT 
AAAAGGCTAG GAAAGAGATT CAAGAAGCTT GACTTGGACA ACTCTGGTTC TTTGAGCGTG 
GAGGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAACCCTT TAGTACAGCG GGTCATAGAT 
ATATTCGACA CAGACGGGAA TGGAGAAGTG GACTTCAAAG AATTCATTGA AGGAGTCTCT 
CAGTTCAGTG TCAAAGGCGA TAAGGAACAG AAGTTGAGGT TCGCTTTCCG TATCTACGAC 
ATGGATAAAG ACGGCTATAT TTCCAATGGA GAGCTCTTCC AGGTGTTGAA GATGATGGTG 
GGCAACAACC TGAAAGATAC GCAGTTACAG CAGATTGTAG ACAAAACCAT AATAAACGCA 
GATAAGGACG GGGACGGGAG AATATCCTTT GAGGAGTTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTGGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059005.1
CDS	210..722
Translation	MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_017309.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017309.2

ORF Insert Sequence:

ATGGGAAATG AGGCGAGTTA CCCTTTGGAA ATGTGCTCAC ACTTCGATGC TGATGAGATT 
AAAAGGCTAG GAAAGAGATT CAAGAAGCTT GACTTGGACA ACTCTGGTTC TTTGAGCGTG 
GAGGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAACCCTT TAGTACAGCG GGTCATAGAT 
ATATTCGACA CAGACGGGAA TGGAGAAGTG GACTTCAAAG AATTCATTGA AGGAGTCTCT 
CAGTTCAGTG TCAAAGGCGA TAAGGAACAG AAGTTGAGGT TCGCTTTCCG TATCTACGAC 
ATGGATAAAG ACGGCTATAT TTCCAATGGA GAGCTCTTCC AGGTGTTGAA GATGATGGTG 
GGCAACAACC TGAAAGATAC GCAGTTACAG CAGATTGTAG ACAAAACCAT AATAAACGCA 
GATAAGGACG GGGACGGGAG AATATCCTTT GAGGAGTTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTGGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp3r1 ( XM_008770426.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768648.1 Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43379
Clone ID Related Accession (Same CDS sequence)	XM_008770426.2
Accession Version	XM_008770426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcineurin subunit B type 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG TAAGTTCTGG AGCATCAGTA CCGGGAGAAG TCTCCACACA CCCATACGTT 
TTCGATGCTG ATGAGATTAA AAGGCTAGGA AAGAGATTCA AGAAGCTTGA CTTGGACAAC 
TCTGGTTCTT TGAGCGTGGA GGAGTTCATG TCTCTGCCTG AGTTACAACA GAACCCTTTA 
GTACAGCGGG TCATAGATAT ATTCGACACA GACGGGAATG GAGAAGTGGA CTTCAAAGAA 
TTCATTGAAG GAGTCTCTCA GTTCAGTGTC AAAGGCGATA AGGAACAGAA GTTGAGGTTC 
GCTTTCCGTA TCTACGACAT GGATAAAGAC GGCTATATTT CCAATGGAGA GCTCTTCCAG 
GTGTTGAAGA TGATGGTGGG CAACAACCTG AAAGATACGC AGTTACAGCA GATTGTAGAC 
AAAACCATAA TAAACGCAGA TAAGGACGGG GACGGGAGAA TATCCTTTGA GGAGTTCTGT 
GCTGTTGTAG GTGGCCTAGA TATCCACAAA AAGATGGTGG TGGATGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768648.1
CDS	904..1434
Translation	MASVSSGASVPGEVSTHPYVFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	XM_008770426.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770426.2

ORF Insert Sequence:

ATGGCATCTG TAAGTTCTGG AGCATCAGTA CCGGGAGAAG TCTCCACACA CCCATACGTT 
TTCGATGCTG ATGAGATTAA AAGGCTAGGA AAGAGATTCA AGAAGCTTGA CTTGGACAAC 
TCTGGTTCTT TGAGCGTGGA GGAGTTCATG TCTCTGCCTG AGTTACAACA GAACCCTTTA 
GTACAGCGGG TCATAGATAT ATTCGACACA GACGGGAATG GAGAAGTGGA CTTCAAAGAA 
TTCATTGAAG GAGTCTCTCA GTTCAGTGTC AAAGGCGATA AGGAACAGAA GTTGAGGTTC 
GCTTTCCGTA TCTACGACAT GGATAAAGAC GGCTATATTT CCAATGGAGA GCTCTTCCAG 
GTGTTGAAGA TGATGGTGGG CAACAACCTG AAAGATACGC AGTTACAGCA GATTGTAGAC 
AAAACCATAA TAAACGCAGA TAAGGACGGG GACGGGAGAA TATCCTTTGA GGAGTTCTGT 
GCTGTTGTAG GTGGCCTAGA TATCCACAAA AAGATGGTGG TGGATGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp3r1 ( XM_006251517.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251579.3 Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58271
Clone ID Related Accession (Same CDS sequence)	XM_006251517.3
Accession Version	XM_006251517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcineurin subunit B type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG GAACGGACTT GCAGTCACAG ATTTTCTTCC CTACAGAAAA GAATTTTTGG 
AAAAAAGGCA AAGACCACTT CAGGCAAAAT AAGTACCCAT TCTCGAGGGA ACTTTATAAC 
CTGATTTTTG CTGACAGAAA GGGAAATGAG GCGAGTTACC CTTTGGAAAT GTGCTCACAC 
TTCGATGCTG ATGAGATTAA AAGGCTAGGA AAGAGATTCA AGAAGCTTGA CTTGGACAAC 
TCTGGTTCTT TGAGCGTGGA GGAGTTCATG TCTCTGCCTG AGTTACAACA GAACCCTTTA 
GTACAGCGGG TCATAGATAT ATTCGACACA GACGGGAATG GAGAAGTGGA CTTCAAAGAA 
TTCATTGAAG GAGTCTCTCA GTTCAGTGTC AAAGGCGATA AGGAACAGAA GTTGAGGTTC 
GCTTTCCGTA TCTACGACAT GGATAAAGAC GGCTATATTT CCAATGGAGA GCTCTTCCAG 
GTGTTGAAGA TGATGGTGGG CAACAACCTG AAAGATACGC AGTTACAGCA GATTGTAGAC 
AAAACCATAA TAAACGCAGA TAAGGACGGG GACGGGAGAA TATCCTTTGA GGAGTTCTGT 
GCTGTTGTAG GTGGCCTAGA TATCCACAAA AAGATGGTGG TGGATGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251579.3
CDS	16..666
Translation	MEQGTDLQSQIFFPTEKNFWKKGKDHFRQNKYPFSRELYNLIFADRKGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	XM_006251517.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251517.3

ORF Insert Sequence:

ATGGAGCAAG GAACGGACTT GCAGTCACAG ATTTTCTTCC CTACAGAAAA GAATTTTTGG 
AAAAAAGGCA AAGACCACTT CAGGCAAAAT AAGTACCCAT TCTCGAGGGA ACTTTATAAC 
CTGATTTTTG CTGACAGAAA GGGAAATGAG GCGAGTTACC CTTTGGAAAT GTGCTCACAC 
TTCGATGCTG ATGAGATTAA AAGGCTAGGA AAGAGATTCA AGAAGCTTGA CTTGGACAAC 
TCTGGTTCTT TGAGCGTGGA GGAGTTCATG TCTCTGCCTG AGTTACAACA GAACCCTTTA 
GTACAGCGGG TCATAGATAT ATTCGACACA GACGGGAATG GAGAAGTGGA CTTCAAAGAA 
TTCATTGAAG GAGTCTCTCA GTTCAGTGTC AAAGGCGATA AGGAACAGAA GTTGAGGTTC 
GCTTTCCGTA TCTACGACAT GGATAAAGAC GGCTATATTT CCAATGGAGA GCTCTTCCAG 
GTGTTGAAGA TGATGGTGGG CAACAACCTG AAAGATACGC AGTTACAGCA GATTGTAGAC 
AAAACCATAA TAAACGCAGA TAAGGACGGG GACGGGAGAA TATCCTTTGA GGAGTTCTGT 
GCTGTTGTAG GTGGCCTAGA TATCCACAAA AAGATGGTGG TGGATGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp3r1 ( XM_017599150.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454639.1 Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa58272
Clone ID Related Accession (Same CDS sequence)	XM_017599150.1
Accession Version	XM_017599150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calcineurin subunit B type 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACTA CTCAATTCCT CTGGACAGTT AGTAATCAAC TGTTTGGAAA TGAGGCGAGT 
TACCCTTTGG AAATGTGCTC ACACTTCGAT GCTGATGAGA TTAAAAGGCT AGGAAAGAGA 
TTCAAGAAGC TTGACTTGGA CAACTCTGGT TCTTTGAGCG TGGAGGAGTT CATGTCTCTG 
CCTGAGTTAC AACAGAACCC TTTAGTACAG CGGGTCATAG ATATATTCGA CACAGACGGG 
AATGGAGAAG TGGACTTCAA AGAATTCATT GAAGGAGTCT CTCAGTTCAG TGTCAAAGGC 
GATAAGGAAC AGAAGTTGAG GTTCGCTTTC CGTATCTACG ACATGGATAA AGACGGCTAT 
ATTTCCAATG GAGAGCTCTT CCAGGTGTTG AAGATGATGG TGGGCAACAA CCTGAAAGAT 
ACGCAGTTAC AGCAGATTGT AGACAAAACC ATAATAAACG CAGATAAGGA CGGGGACGGG 
AGAATATCCT TTGAGGAGTT CTGTGCTGTT GTAGGTGGCC TAGATATCCA CAAAAAGATG 
GTGGTGGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454639.1
CDS	1059..1613
Translation	MKTTQFLWTVSNQLFGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	XM_017599150.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 3, regulatory subunit B, alpha (Ppp3r1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599150.1

ORF Insert Sequence:

ATGAAAACTA CTCAATTCCT CTGGACAGTT AGTAATCAAC TGTTTGGAAA TGAGGCGAGT 
TACCCTTTGG AAATGTGCTC ACACTTCGAT GCTGATGAGA TTAAAAGGCT AGGAAAGAGA 
TTCAAGAAGC TTGACTTGGA CAACTCTGGT TCTTTGAGCG TGGAGGAGTT CATGTCTCTG 
CCTGAGTTAC AACAGAACCC TTTAGTACAG CGGGTCATAG ATATATTCGA CACAGACGGG 
AATGGAGAAG TGGACTTCAA AGAATTCATT GAAGGAGTCT CTCAGTTCAG TGTCAAAGGC 
GATAAGGAAC AGAAGTTGAG GTTCGCTTTC CGTATCTACG ACATGGATAA AGACGGCTAT 
ATTTCCAATG GAGAGCTCTT CCAGGTGTTG AAGATGATGG TGGGCAACAA CCTGAAAGAT 
ACGCAGTTAC AGCAGATTGT AGACAAAACC ATAATAAACG CAGATAAGGA CGGGGACGGG 
AGAATATCCT TTGAGGAGTT CTGTGCTGTT GTAGGTGGCC TAGATATCCA CAAAAAGATG 
GTGGTGGATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Investigating the human Calcineurin Interaction Network using the ??LxVP SLiM.
Scientific reports638920(2016 Dec)
Sheftic SR,Page R,Peti W

Cooperative autoinhibition and multi-level activation mechanisms of calcineurin.
Cell research26(3)336-49(2016 Mar)
Li SJ,Wang J,Ma L,Lu C,Wang J,Wu JW,Wang ZX

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
The EMBO journal33(2)157-70(2014 Jan)
Biesemann C,Gr?nborg M,Luquet E,Wichert SP,Bernard V,Bungers SR,Cooper B,Varoqueaux F,Li L,Byrne JA,Urlaub H,Jahn O,Brose N,Herzog E

Structural basis of calcineurin activation by calmodulin.
Cellular signalling25(12)2661-7(2013 Dec)
Ye Q,Feng Y,Yin Y,Faucher F,Currie MA,Rahman MN,Jin J,Li S,Wei Q,Jia Z

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Ppp3r1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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