Nagk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nagk
Entrez Gene ID	297393
Full Name	N-acetylglucosamine kinase
General protein information	Preferred Names N-acetylglucosamine kinase Names N-acetyl-D-glucosamine kinase glcNAc kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037768.1	NP_001032857.1	N-acetyl-D-glucosamine kinase
XM_006236740.3	XP_006236802.1	N-acetyl-D-glucosamine kinase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NAGK	NP_001029486.1
Mus musculus (house mouse)	Nagk	NP_062415.1
Drosophila melanogaster (fruit fly)	CG6218	NP_650448.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000241	XP_310885.4
Pan troglodytes (chimpanzee)	NAGK	XP_515537.3
Caenorhabditis elegans (roundworm)	W06B4.2	NP_494789.1
Homo sapiens (human)	NAGK	NP_060037.3
	NAGK	XP_001102037.2
Xenopus tropicalis (tropical clawed frog)	LOC100485993	XP_002938443.1
Canis lupus familiaris (dog)	NAGK	XP_549180.3
Rattus norvegicus (Norway rat)	Nagk	NP_001032857.1
Danio rerio (zebrafish)	zgc:171682	NP_001099163.1

Gene Ontology
Bibliography

Related articles in PubMed

N-Acetyl-D-Glucosamine Kinase Promotes the Axonal Growth of Developing Neurons.
Islam MA, Sharif SR, Lee H, Moon IS
Molecules and cells38(10)876-85(2015 Oct)

N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points.
Islam MA, Sharif SR, Lee H, Seog DH, Moon IS
Experimental & molecular medicine47e177(2015 Aug)

N-acetyl-D-glucosamine kinase is a component of nuclear speckles and paraspeckles.
Sharif SR, Lee H, Islam MA, Seog DH, Moon IS
Molecules and cells38(5)402-8(2015 May)

The non-canonical effect of N-acetyl-D-glucosamine kinase on the formation of neuronal dendrites.
Lee H, Cho SJ, Moon IS
Molecules and cells37(3)248-56(2014 Mar)

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
Bergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A
The Journal of biological chemistry287(34)28865-81(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this work studied the role played by NAGK in axonal development.
Title: N-Acetyl-D-Glucosamine Kinase Promotes the Axonal Growth of Developing Neurons.

The interaction between NAGK and Dynlrb1 frequently occurs at dendritic branch points.
Title: N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points.

Data shows associations between NAGK, speckle, paraspeckle and general transcription factor suggesting its regulatory roles in gene expression.
Title: N-acetyl-D-glucosamine kinase is a component of nuclear speckles and paraspeckles.

Upregulation of dendritic complexity is a non-canonical function of NAGK.
Title: The non-canonical effect of N-acetyl-D-glucosamine kinase on the formation of neuronal dendrites.

analysis of oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods
Title: Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods.

The following Nagk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nagk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12435	NM_001037768.1 Latest version!	Rattus norvegicus N-acetylglucosamine kinase (Nagk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa27386	XM_006236740.3 Latest version!	Rattus norvegicus N-acetylglucosamine kinase (Nagk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nagk ( NM_001037768.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001032857.1 Rattus norvegicus N-acetylglucosamine kinase (Nagk), mRNA.

Price & Availability↑

CloneID	ORa12435
Clone ID Related Accession (Same CDS sequence)	NM_001037768.1
Accession Version	NM_001037768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	N-acetyl-D-glucosamine kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107647.1. On Dec 16, 2005 this sequence version replaced XM_216188.3. ##Evidence-Data-START## Transcript exon combination :: BC107647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCAC TTTATGGTGG CGTGGAAGGG GGAGGCACAC GGTCCAAAGT CCTTTTACTT 
TCTGAGGATG GGCAGATCCT GGCAGAAGCA GATGGACTGA GCACAAATCA CTGGCTGATT 
GGCACAGGTA CCTGTGTGGA GAGGATCAAT GAGATGGTGG ACAGGGCTAA ACGGAAGGCT 
GGAGTGGATC CTCTGGTACC CCTTCGAAGC CTGGGCTTGT CCCTGAGTGG TGGGGAGCAG 
GAGGATGCAG TGAGGCTCCT GATGGAGGAG TTGAGGGACC GATTTCCCTA CCTGAGTGAA 
AGTTACTTCA TCACCACTGA TGCAGCAGGT TCCATCGCCA CAGCTACACC GGATGGTGGG 
ATTGTGCTCA TCTCTGGAAC AGGCTCCAAC TGTAGGCTTA TCAACCCTGA TGGCTCTGAG 
AGTGGCTGTG GTGGCTGGGG CCACATGATG GGAGACGAGG GATCAGCCTA CTGGATTGCA 
CACCAAGCTG TGAAAATTGT GTTTGACTCC ATTGACAACC TGGAAGCAGC TCCTCATGAT 
ATTGGCCATG TCAAGCAGGC CATGTTCAAC TACTTCCAGG TGCCAGATCG GCTAGGAATC 
CTCACTCACT TGTATAGGGA CTTTGATAAG TCCAAGTTTG CTGGATTTTG TCAGAAAATT 
GCAGAAGGTG CACAGCAGGG AGACCCTCTT TCCAGGTTCA TCTTCAGAAA GGCTGGGGAG 
ATGCTGGGCA GACACGTTGT GGCAGTATTG CCAGAGATTG ACCCAGTTTT GTTCCAAGGG 
GAGCTTGGCC TCCCCATTCT GTGTGTGGGC TCAGTGTGGA AGAGCTGGGA GCTACTGAAG 
GAAGGCTTTC TCCTGGCACT GACGCAGGGC CGAGAGCAAC AGGCACAGAA CTCCTTCTCC 
AGTTTCACCC TGATGAAGTT GAGGCACTCT TCTGCACTGG GAGGGGCCAG CCTGGGGGCC 
AGGCACATTG GACACCACCT TCCCATGGAC TACAGCGTCA ATGCCATTGC CTTCTATTCC 
TATACCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032857.1
CDS	38..1069
Translation	MAALYGGVEGGGTRSKVLLLSEDGQILAEADGLSTNHWLIGTGTCVERINEMVDRAKRKAGVDPLVPLRSLGLSLSGGEQEDAVRLLMEELRDRFPYLSESYFITTDAAGSIATATPDGGIVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGHVKQAMFNYFQVPDRLGILTHLYRDFDKSKFAGFCQKIAEGAQQGDPLSRFIFRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVWKSWELLKEGFLLALTQGREQQAQNSFSSFTLMKLRHSSALGGASLGARHIGHHLPMDYSVNAIAFYSYTF

Target ORF information:

RefSeq Version	NM_001037768.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-acetylglucosamine kinase (Nagk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037768.1

ORF Insert Sequence:

ATGGCCGCAC TTTATGGTGG CGTGGAAGGG GGAGGCACAC GGTCCAAAGT CCTTTTACTT 
TCTGAGGATG GGCAGATCCT GGCAGAAGCA GATGGACTGA GCACAAATCA CTGGCTGATT 
GGCACAGGTA CCTGTGTGGA GAGGATCAAT GAGATGGTGG ACAGGGCTAA ACGGAAGGCT 
GGAGTGGATC CTCTGGTACC CCTTCGAAGC CTGGGCTTGT CCCTGAGTGG TGGGGAGCAG 
GAGGATGCAG TGAGGCTCCT GATGGAGGAG TTGAGGGACC GATTTCCCTA CCTGAGTGAA 
AGTTACTTCA TCACCACTGA TGCAGCAGGT TCCATCGCCA CAGCTACACC GGATGGTGGG 
ATTGTGCTCA TCTCTGGAAC AGGCTCCAAC TGTAGGCTTA TCAACCCTGA TGGCTCTGAG 
AGTGGCTGTG GTGGCTGGGG CCACATGATG GGAGACGAGG GATCAGCCTA CTGGATTGCA 
CACCAAGCTG TGAAAATTGT GTTTGACTCC ATTGACAACC TGGAAGCAGC TCCTCATGAT 
ATTGGCCATG TCAAGCAGGC CATGTTCAAC TACTTCCAGG TGCCAGATCG GCTAGGAATC 
CTCACTCACT TGTATAGGGA CTTTGATAAG TCCAAGTTTG CTGGATTTTG TCAGAAAATT 
GCAGAAGGTG CACAGCAGGG AGACCCTCTT TCCAGGTTCA TCTTCAGAAA GGCTGGGGAG 
ATGCTGGGCA GACACGTTGT GGCAGTATTG CCAGAGATTG ACCCAGTTTT GTTCCAAGGG 
GAGCTTGGCC TCCCCATTCT GTGTGTGGGC TCAGTGTGGA AGAGCTGGGA GCTACTGAAG 
GAAGGCTTTC TCCTGGCACT GACGCAGGGC CGAGAGCAAC AGGCACAGAA CTCCTTCTCC 
AGTTTCACCC TGATGAAGTT GAGGCACTCT TCTGCACTGG GAGGGGCCAG CCTGGGGGCC 
AGGCACATTG GACACCACCT TCCCATGGAC TACAGCGTCA ATGCCATTGC CTTCTATTCC 
TATACCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nagk ( XM_006236740.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236802.1 Rattus norvegicus N-acetylglucosamine kinase (Nagk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27386
Clone ID Related Accession (Same CDS sequence)	XM_006236740.3
Accession Version	XM_006236740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-acetyl-D-glucosamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAC TTTATGGTGG CGTGGAAGGG GGAGGCACAC GGTCCAAAGT CCTTTTACTT 
TCTGAGGATG GGCAGATCCT GGCAGAAGCA GATGGACTGA GCACAAATCA CTGGCTGATT 
GGCACAGGTA CCTGTGTGGA GAGGATCAAT GAGATGGTGG ACAGGGCTAA ACGGAAGGCT 
GGAGTGGATC CTCTGGTACC CCTTCGAAGC CTGGGCTTGT CCCTGAGTGG TGGGGAGCAG 
GAGGATGCAG TGAGGCTCCT GATGGAGGAG TTGAGGGACC GATTTCCCTA CCTGAGTGAA 
AGTTACTTCA TCACCACTGA TGCAGCAGGT TCCATCGCCA CAGCTACACC GGATGGTGGG 
ATTGTGCTCA TCTCTGGAAC AGGCTCCAAC TGTAGGCTTA TCAACCCTGA TGGCTCTGAG 
AGTGGCTGTG GTGGCTGGGG CCACATGATG GGAGACGAGG GATCAGCCTA CTGGATTGCA 
CACCAAGCTG TGAAAATTGT GTTTGACTCC ATTGACAACC TGGAAGCAGC TCCTCATGAT 
ATTGGCCATG TCAAGCAGGC CATGTTCAAC TACTTCCAGG TGCCAGATCG GCTAGGAATC 
CTCACTCACT TGTATAGGGA CTTTGATAAG TCCAAGTTTG CTGGATTTTG TCAGAAAATT 
GCAGAAGGTT TTGTTCCAAG GGGAGCTTGG CCTCCCCATT CTGTGTGTGG GCTCAGTGTG 
GAAGAGCTGG GAGCTACTGA AGGAAGGCTT TCTCCTGGCA CTGACGCAGG GCCGAGAGCA 
ACAGGCACAG AACTCCTTCT CCAGTTTCAC CCTGATGAAG TTGAGGCACT CTTCTGCACT 
GGGAGGGGCC AGCCTGGGGG CCAGGCACAT TGGACACCAC CTTCCCATGG ACTACAGCGT 
CAATGCCATT GCCTTCTATT CCTATACCTT CTAGGGGCTG CCCCTCTTCC CATTCAGCCA 
ACACTGAGTG TTGGGAGATT TCTCTTTTTT AAAAACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236802.1
CDS	222..1223
Translation	MAALYGGVEGGGTRSKVLLLSEDGQILAEADGLSTNHWLIGTGTCVERINEMVDRAKRKAGVDPLVPLRSLGLSLSGGEQEDAVRLLMEELRDRFPYLSESYFITTDAAGSIATATPDGGIVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGHVKQAMFNYFQVPDRLGILTHLYRDFDKSKFAGFCQKIAEGFVPRGAWPPHSVCGLSVEELGATEGRLSPGTDAGPRATGTELLLQFHPDEVEALFCTGRGQPGGQAHWTPPSHGLQRQCHCLLFLYLLGAAPLPIQPTLSVGRFLFFKNT

Target ORF information:

RefSeq Version	XM_006236740.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-acetylglucosamine kinase (Nagk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236740.3

ORF Insert Sequence:

ATGGCCGCAC TTTATGGTGG CGTGGAAGGG GGAGGCACAC GGTCCAAAGT CCTTTTACTT 
TCTGAGGATG GGCAGATCCT GGCAGAAGCA GATGGACTGA GCACAAATCA CTGGCTGATT 
GGCACAGGTA CCTGTGTGGA GAGGATCAAT GAGATGGTGG ACAGGGCTAA ACGGAAGGCT 
GGAGTGGATC CTCTGGTACC CCTTCGAAGC CTGGGCTTGT CCCTGAGTGG TGGGGAGCAG 
GAGGATGCAG TGAGGCTCCT GATGGAGGAG TTGAGGGACC GATTTCCCTA CCTGAGTGAA 
AGTTACTTCA TCACCACTGA TGCAGCAGGT TCCATCGCCA CAGCTACACC GGATGGTGGG 
ATTGTGCTCA TCTCTGGAAC AGGCTCCAAC TGTAGGCTTA TCAACCCTGA TGGCTCTGAG 
AGTGGCTGTG GTGGCTGGGG CCACATGATG GGAGACGAGG GATCAGCCTA CTGGATTGCA 
CACCAAGCTG TGAAAATTGT GTTTGACTCC ATTGACAACC TGGAAGCAGC TCCTCATGAT 
ATTGGCCATG TCAAGCAGGC CATGTTCAAC TACTTCCAGG TGCCAGATCG GCTAGGAATC 
CTCACTCACT TGTATAGGGA CTTTGATAAG TCCAAGTTTG CTGGATTTTG TCAGAAAATT 
GCAGAAGGTT TTGTTCCAAG GGGAGCTTGG CCTCCCCATT CTGTGTGTGG GCTCAGTGTG 
GAAGAGCTGG GAGCTACTGA AGGAAGGCTT TCTCCTGGCA CTGACGCAGG GCCGAGAGCA 
ACAGGCACAG AACTCCTTCT CCAGTTTCAC CCTGATGAAG TTGAGGCACT CTTCTGCACT 
GGGAGGGGCC AGCCTGGGGG CCAGGCACAT TGGACACCAC CTTCCCATGG ACTACAGCGT 
CAATGCCATT GCCTTCTATT CCTATACCTT CTAGGGGCTG CCCCTCTTCC CATTCAGCCA 
ACACTGAGTG TTGGGAGATT TCTCTTTTTT AAAAACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-Acetyl-D-Glucosamine Kinase Promotes the Axonal Growth of Developing Neurons.
Molecules and cells38(10)876-85(2015 Oct)
Islam MA,Sharif SR,Lee H,Moon IS

N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points.
Experimental & molecular medicine47e177(2015 Aug)
Islam MA,Sharif SR,Lee H,Seog DH,Moon IS

N-acetyl-D-glucosamine kinase is a component of nuclear speckles and paraspeckles.
Molecules and cells38(5)402-8(2015 May)
Sharif SR,Lee H,Islam MA,Seog DH,Moon IS

The non-canonical effect of N-acetyl-D-glucosamine kinase on the formation of neuronal dendrites.
Molecules and cells37(3)248-56(2014 Mar)
Lee H,Cho SJ,Moon IS

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
The Journal of biological chemistry287(34)28865-81(2012 Aug)
Bergfeld AK,Pearce OM,Diaz SL,Pham T,Varki A

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Nagk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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