Gclm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gclm
Entrez Gene ID	29739
Full Name	glutamate cysteine ligase, modifier subunit
Synonyms	Glclr
General protein information	Preferred Names glutamate cysteine ligase, modifier subunit Names glutamate--cysteine ligase regulatory subunit GCS light chain gamma-ECS regulatory subunit gamma-glutamylcysteine synthetase regulatory subunit glutamate cysteine ligase (gamma-glutamylcysteine synthetase), regulatory
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q41
Summary	an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017305.2	NP_059001.1	glutamate--cysteine ligase regulatory subunit

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GCLM	NP_001033232.1
Gallus gallus (chicken)	GCLM	NP_001007954.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012038	XP_320488.3
Caenorhabditis elegans (roundworm)	E01A2.1	NP_001122439.1
Pan troglodytes (chimpanzee)	GCLM	XP_513572.3
Danio rerio (zebrafish)	gclm	NP_956139.1
Homo sapiens (human)	GCLM	NP_002052.1
Canis lupus familiaris (dog)	GCLM	XP_005621954.1
Xenopus tropicalis (tropical clawed frog)	gclm	NP_001016536.1
Mus musculus (house mouse)	Gclm	NP_032155.1
Rattus norvegicus (Norway rat)	Gclm	NP_059001.1
Drosophila melanogaster (fruit fly)	Gclm	NP_001262845.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.
Zhang S, Li H, Zhang L, Li J, Wang R, Wang M
Brain research1657355-360(2017 02)

Schisandra Chinensis Baillon regulates the gene expression of phase II antioxidant/detoxifying enzymes in hepatic damage induced rats.
Jang HI, Do GM, Lee HM, Ok HM, Shin JH, Kwon O
Nutrition research and practice8(3)272-7(2014 Jun)

Upregulation of capacity for glutathione synthesis in response to amino acid deprivation: regulation of glutamate-cysteine ligase subunits.
Sikalidis AK, Mazor KM, Lee JI, Roman HB, Hirschberger LL, Stipanuk MH
Amino acids46(5)1285-96(2014 May)

Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis.
Ramani K, Tomasi ML, Yang H, Ko K, Lu SC
The Journal of biological chemistry287(43)36341-55(2012 Oct)

Rice protein improves oxidative stress by regulating glutathione metabolism and attenuating oxidative damage to lipids and proteins in rats.
Yang L, Chen JH, Xu T, Zhou AS, Yang HK
Life sciences91(11-12)389-394(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study determined the effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits (GCLM and GCLC) in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rat: troxerutin alleviates oxidative stress and promotes learning potential in STZ-induced diabetic rats likely through GCL mechanisms, especially that of GCLC.
Title: Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.

Upregulation of both GCLC and GLCM mRNA levels in response to cysteine deprivation was dependent on new protein synthesis.
Title: Upregulation of capacity for glutathione synthesis in response to amino acid deprivation: regulation of glutamate-cysteine ligase subunits.

Thus, the present results indicate that the vitamin E-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.
Title: Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver.

the catalytic efficiency of rat GCL is increased by holoenzyme formation and the first demonstration of differential up-regulation of the GCL subunits in response to cysteine deprivation
Title: Differential regulation of glutamate-cysteine ligase subunit expression and increased holoenzyme formation in response to cysteine deprivation.

specific and independent disruption of each subunit of GCL in neurons can be said to cause a primary decrease in glutathione that is sufficient to promote neurodegeneration
Title: Knockdown of glutamate-cysteine ligase by small hairpin RNA reveals that both catalytic and modulatory subunits are essential for the survival of primary neurons.

The following Gclm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gclm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03601	NM_017305.2 Latest version!	Rattus norvegicus glutamate cysteine ligase, modifier subunit (Gclm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gclm ( NM_017305.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059001.1 Rattus norvegicus glutamate cysteine ligase, modifier subunit (Gclm), mRNA.

Price & Availability↑

CloneID	ORa03601
Clone ID Related Accession (Same CDS sequence)	NM_017305.2
Accession Version	NM_017305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	glutamate--cysteine ligase regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078867.1. On Aug 8, 2004 this sequence version replaced NM_017305.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078867.1, L22191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACCG ACAGCCGCGC GGCCGGAGCA CTTCTGGCGC GGGCCAGCAC CCTGCACCTG 
CAGACCGGGA ACCTGCTCAA CTGGGGCCGC CTGCGGAAAA AGTGTCCGTC CACGCACAGC 
GAGGAGCTTC GAGACTGTAT CCAAAAGACC TTGAATGAAT GGAGCTCCCA AATCAGCCCT 
GATTTGGTCA GGGAGTTTCC AGATGTTTTG GAATGTACCA TGTCCCATGC AGTGGAAAAG 
ATAAACCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCTA AACTGTTCAT TGTAGGATCG 
AATTCTTCAT CATCAACTAG AAATGCAGTT GACATGGCAT GCTCAGTCCT TGGAGTTGCA 
CAGCTGGACT CTGTCATCAT GGCTTCCCCT CCAATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTGC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA GAAGATTGTT 
GCTATAGGCA CCTCGGATCT AGACAAAACA CAGTTGGAGC AGCTGTACCA GTGGGCACAG 
GTAAAACCCA ATAGTAATCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAGTT TGACATACAG CTACTGACTC ACAATGACCC AAAAGAACTG 
CTCTCTGAGG CAAGTTTCCA GGAAGCTCTT CAAGAAAGCA TCCCTGACAT TGAAGCCCAG 
GAGTGGGTGC CACTGTGGCT GCTGAGGTAC TCGGTCATCG TGAAAAGCAG AGGAATCATC 
AAGTCAAAAG GATACATTTT GCAAGCCAAA AGAAAGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059001.1
CDS	123..947
Translation	MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRKGS

Target ORF information:

RefSeq Version	NM_017305.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate cysteine ligase, modifier subunit (Gclm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017305.2

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCGGAGCA CTTCTGGCGC GGGCCAGCAC CCTGCACCTG 
CAGACCGGGA ACCTGCTCAA CTGGGGCCGC CTGCGGAAAA AGTGTCCGTC CACGCACAGC 
GAGGAGCTTC GAGACTGTAT CCAAAAGACC TTGAATGAAT GGAGCTCCCA AATCAGCCCT 
GATTTGGTCA GGGAGTTTCC AGATGTTTTG GAATGTACCA TGTCCCATGC AGTGGAAAAG 
ATAAACCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCTA AACTGTTCAT TGTAGGATCG 
AATTCTTCAT CATCAACTAG AAATGCAGTT GACATGGCAT GCTCAGTCCT TGGAGTTGCA 
CAGCTGGACT CTGTCATCAT GGCTTCCCCT CCAATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTGC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA GAAGATTGTT 
GCTATAGGCA CCTCGGATCT AGACAAAACA CAGTTGGAGC AGCTGTACCA GTGGGCACAG 
GTAAAACCCA ATAGTAATCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAGTT TGACATACAG CTACTGACTC ACAATGACCC AAAAGAACTG 
CTCTCTGAGG CAAGTTTCCA GGAAGCTCTT CAAGAAAGCA TCCCTGACAT TGAAGCCCAG 
GAGTGGGTGC CACTGTGGCT GCTGAGGTAC TCGGTCATCG TGAAAAGCAG AGGAATCATC 
AAGTCAAAAG GATACATTTT GCAAGCCAAA AGAAAGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of troxerutin on cognitive deficits and glutamate cysteine ligase subunits in the hippocampus of streptozotocin-induced type 1 diabetes mellitus rats.
Brain research1657355-360(2017 02)
Zhang S,Li H,Zhang L,Li J,Wang R,Wang M

Schisandra Chinensis Baillon regulates the gene expression of phase II antioxidant/detoxifying enzymes in hepatic damage induced rats.
Nutrition research and practice8(3)272-7(2014 Jun)
Jang HI,Do GM,Lee HM,Ok HM,Shin JH,Kwon O

Upregulation of capacity for glutathione synthesis in response to amino acid deprivation: regulation of glutamate-cysteine ligase subunits.
Amino acids46(5)1285-96(2014 May)
Sikalidis AK,Mazor KM,Lee JI,Roman HB,Hirschberger LL,Stipanuk MH

Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis.
The Journal of biological chemistry287(43)36341-55(2012 Oct)
Ramani K,Tomasi ML,Yang H,Ko K,Lu SC

Rice protein improves oxidative stress by regulating glutathione metabolism and attenuating oxidative damage to lipids and proteins in rats.
Life sciences91(11-12)389-394(2012 Oct)
Yang L,Chen JH,Xu T,Zhou AS,Yang HK

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Gclm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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