Chchd3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chchd3
Entrez Gene ID	296966
Full Name	coiled-coil-helix-coiled-coil-helix domain containing 3
General protein information	Preferred Names coiled-coil-helix-coiled-coil-helix domain containing 3 Names MICOS complex subunit MIC19 coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592526.1	XP_017448015.1	MICOS complex subunit MIC19 isoform X2
XM_017592525.1	XP_017448014.1	MICOS complex subunit MIC19 isoform X1
NM_001106588.1	NP_001100058.1	MICOS complex subunit MIC19

Pathways from BioSystems

Homologies

	CHCHD3	XP_001099844.1
Gallus gallus (chicken)	CHCHD3	XP_416646.3
Danio rerio (zebrafish)	chchd3	NP_999969.1
Rattus norvegicus (Norway rat)	Chchd3	NP_001100058.1
Pan troglodytes (chimpanzee)	CHCHD3	XP_001136966.1
Canis lupus familiaris (dog)	CHCHD3	XP_848455.2
Homo sapiens (human)	CHCHD3	NP_060282.1
Bos taurus (cattle)	CHCHD3	NP_001030552.1
Mus musculus (house mouse)	Chchd3	NP_079612.1
Xenopus tropicalis (tropical clawed frog)	chchd3	NP_001004897.1

Gene Ontology
Bibliography

Related articles in PubMed

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.
Guarani V, McNeill EM, Paulo JA, Huttlin EL, Fr?hlich F, Gygi SP, Van Vactor D, Harper JW
eLife4(2015 May)

Detailed analysis of the human mitochondrial contact site complex indicate a hierarchy of subunits.
Ott C, Dorsch E, Fraunholz M, Straub S, Kozjak-Pavlovic V
PloS one10(3)e0120213(2015)

Mitofilin complexes: conserved organizers of mitochondrial membrane architecture.
Zerbes RM, van der Klei IJ, Veenhuis M, Pfanner N, van der Laan M, Bohnert M
Biological chemistry393(11)1247-61(2012 Nov)

Cloning and functional analysis of FLJ20420: a novel transcription factor for the BAG-1 promoter.
Liu H, Li Y, Li Y, Liu B, Wu H, Wang J, Wang Y, Wang M, Tang SC, Zhou Q, Chen J
PloS one7(5)e34832(2012)

ChChd3, an inner mitochondrial membrane protein, is essential for maintaining crista integrity and mitochondrial function.
Darshi M, Mendiola VL, Mackey MR, Murphy AN, Koller A, Perkins GA, Ellisman MH, Taylor SS
The Journal of biological chemistry286(4)2918-32(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chchd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chchd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04827	NM_001106588.1 Latest version!	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa60798	XM_017592525.1 Latest version!	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa60799	XM_017592526.1 Latest version!	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chchd3 ( NM_001106588.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100058.1 Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), mRNA.

Price & Availability↑

CloneID	ORa04827
Clone ID Related Accession (Same CDS sequence)	NM_001106588.1
Accession Version	NM_001106588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	MICOS complex subunit MIC19
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473959.1. On or before Oct 4, 2007 this sequence version replaced XM_001066573.1, XM_238346.4. ##Evidence-Data-START## Transcript exon combination :: FQ216356.1, FQ215338.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGG CTCGGCAGCT GGAAGAGAAA GATCGAATGA TACGGAAGCA GGATGCGTTC 
TACAAAGAGC AGCTGTCCAG ACTGGAGGAG AGGAGCTCAG AATTCTACAA AGTTACTACC 
GAGGAATATC AGAAAGCTGC TGAAGAGGTG GAGTCCAAGT TCAGGCGGTA TGAATATCAT 
CCAGTCTGTG CAGATCTGCA GACCAAAATC CTTCAGTGTT ACCGTCAGAA CACCCAGCAG 
ACCCTCAGCT GCTCTGCTCT GGCCAACCAA TACATGCACT GTGTCAACCA TGCCAAACAG 
AACATGCTGG AGAAGGGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100058.1
CDS	160..843
Translation	MGGTASTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSSVYGASVSDEELKRRVAEELALEQAKMESENQRRLKHARDLEREKAVANEQLTRAILQERISSEENRTKVKHLARQLEEKDRMIRKQDAFYKEQLSRLEERSSEFYKVTTEEYQKAAEEVESKFRRYEYHPVCADLQTKILQCYRQNTQQTLSCSALANQYMHCVNHAKQNMLEKGG

Target ORF information:

RefSeq Version	NM_001106588.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106588.1

ORF Insert Sequence:

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGG CTCGGCAGCT GGAAGAGAAA GATCGAATGA TACGGAAGCA GGATGCGTTC 
TACAAAGAGC AGCTGTCCAG ACTGGAGGAG AGGAGCTCAG AATTCTACAA AGTTACTACC 
GAGGAATATC AGAAAGCTGC TGAAGAGGTG GAGTCCAAGT TCAGGCGGTA TGAATATCAT 
CCAGTCTGTG CAGATCTGCA GACCAAAATC CTTCAGTGTT ACCGTCAGAA CACCCAGCAG 
ACCCTCAGCT GCTCTGCTCT GGCCAACCAA TACATGCACT GTGTCAACCA TGCCAAACAG 
AACATGCTGG AGAAGGGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chchd3 ( XM_017592525.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448014.1 Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60798
Clone ID Related Accession (Same CDS sequence)	XM_017592525.1
Accession Version	XM_017592525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	MICOS complex subunit MIC19 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGG ATATTGAAGA CAAAGCTCGG CAGCTGGAAG AGAAAGATCG AATGATACGG 
AAGCAGGATG CGTTCTACAA AGAGCAGCTG TCCAGACTGG AGGAGAGGAG CTCAGAATTC 
TACAAAGTTA CTACCGAGGA ATATCAGAAA GCTGCTGAAG AGGTGGAGTC CAAGTTCAGG 
CGGTATGAAT ATCATCCAGT CTGTGCAGAT CTGCAGACCA AAATCCTTCA GTGTTACCGT 
CAGAACACCC AGCAGACCCT CAGCTGCTCT GCTCTGGCCA ACCAATACAT GCACTGTGTC 
AACCATGCCA AACAGAACAT GCTGGAGAAG GGAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448014.1
CDS	190..888
Translation	MGGTASTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSSVYGASVSDEELKRRVAEELALEQAKMESENQRRLKHARDLEREKAVANEQLTRAILQERISSEENRTKVKHLDIEDKARQLEEKDRMIRKQDAFYKEQLSRLEERSSEFYKVTTEEYQKAAEEVESKFRRYEYHPVCADLQTKILQCYRQNTQQTLSCSALANQYMHCVNHAKQNMLEKGG

Target ORF information:

RefSeq Version	XM_017592525.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592525.1

ORF Insert Sequence:

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGG ATATTGAAGA CAAAGCTCGG CAGCTGGAAG AGAAAGATCG AATGATACGG 
AAGCAGGATG CGTTCTACAA AGAGCAGCTG TCCAGACTGG AGGAGAGGAG CTCAGAATTC 
TACAAAGTTA CTACCGAGGA ATATCAGAAA GCTGCTGAAG AGGTGGAGTC CAAGTTCAGG 
CGGTATGAAT ATCATCCAGT CTGTGCAGAT CTGCAGACCA AAATCCTTCA GTGTTACCGT 
CAGAACACCC AGCAGACCCT CAGCTGCTCT GCTCTGGCCA ACCAATACAT GCACTGTGTC 
AACCATGCCA AACAGAACAT GCTGGAGAAG GGAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chchd3 ( XM_017592526.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448015.1 Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60799
Clone ID Related Accession (Same CDS sequence)	XM_017592526.1
Accession Version	XM_017592526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	MICOS complex subunit MIC19 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGA GCTCAGAATT CTACAAAGTT ACTACCGAGG AATATCAGAA AGCTGCTGAA 
GAGGTGGAGT CCAAGTTCAG GCGGTATGAA TATCATCCAG TCTGTGCAGA TCTGCAGACC 
AAAATCCTTC AGTGTTACCG TCAGAACACC CAGCAGACCC TCAGCTGCTC TGCTCTGGCC 
AACCAATACA TGCACTGTGT CAACCATGCC AAACAGAACA TGCTGGAGAA GGGAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448015.1
CDS	190..789
Translation	MGGTASTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSSVYGASVSDEELKRRVAEELALEQAKMESENQRRLKHARDLEREKAVANEQLTRAILQERISSEENRTKVKHLSSEFYKVTTEEYQKAAEEVESKFRRYEYHPVCADLQTKILQCYRQNTQQTLSCSALANQYMHCVNHAKQNMLEKGG

Target ORF information:

RefSeq Version	XM_017592526.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coiled-coil-helix-coiled-coil-helix domain containing 3 (Chchd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592526.1

ORF Insert Sequence:

ATGGGCGGGA CCGCTAGCAC CCGCCGGGTC ACCTTCGAGG CGGACGAGAA CGAGAACATC 
ACGGTGGTGA AGGGCATCCG GCTGTCAGAG AATGTGATCG ACCGGATGAA GGAGTCCTCT 
CCATCTGGCT CTAAGTCTCA GCGATATTCC AGTGTTTATG GTGCTTCAGT TTCTGATGAA 
GAATTGAAAA GAAGAGTAGC CGAGGAGCTG GCATTGGAAC AAGCCAAAAT GGAGTCGGAA 
AACCAGAGAC GCCTTAAGCA CGCCAGGGAC CTAGAAAGAG AGAAAGCTGT GGCCAATGAG 
CAGCTGACCA GAGCTATCCT TCAGGAAAGA ATATCCAGTG AGGAGAACCG CACAAAGGTG 
AAGCATCTGA GCTCAGAATT CTACAAAGTT ACTACCGAGG AATATCAGAA AGCTGCTGAA 
GAGGTGGAGT CCAAGTTCAG GCGGTATGAA TATCATCCAG TCTGTGCAGA TCTGCAGACC 
AAAATCCTTC AGTGTTACCG TCAGAACACC CAGCAGACCC TCAGCTGCTC TGCTCTGGCC 
AACCAATACA TGCACTGTGT CAACCATGCC AAACAGAACA TGCTGGAGAA GGGAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.
eLife4(2015 May)
Guarani V,McNeill EM,Paulo JA,Huttlin EL,Fr?hlich F,Gygi SP,Van Vactor D,Harper JW

Detailed analysis of the human mitochondrial contact site complex indicate a hierarchy of subunits.
PloS one10(3)e0120213(2015)
Ott C,Dorsch E,Fraunholz M,Straub S,Kozjak-Pavlovic V

Mitofilin complexes: conserved organizers of mitochondrial membrane architecture.
Biological chemistry393(11)1247-61(2012 Nov)
Zerbes RM,van der Klei IJ,Veenhuis M,Pfanner N,van der Laan M,Bohnert M

Cloning and functional analysis of FLJ20420: a novel transcription factor for the BAG-1 promoter.
PloS one7(5)e34832(2012)
Liu H,Li Y,Li Y,Liu B,Wu H,Wang J,Wang Y,Wang M,Tang SC,Zhou Q,Chen J

ChChd3, an inner mitochondrial membrane protein, is essential for maintaining crista integrity and mitochondrial function.
The Journal of biological chemistry286(4)2918-32(2011 Jan)
Darshi M,Mendiola VL,Mackey MR,Murphy AN,Koller A,Perkins GA,Ellisman MH,Taylor SS

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Chchd3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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