Pla2g2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pla2g2a
Entrez Gene ID	29692
Full Name	phospholipase A2 group IIA
Synonyms	sPLA2
General protein information	Preferred Names phospholipase A2 group IIA Names phospholipase A2, membrane associated GIIC sPLA2 eted enzyme type IIA phospholipase A2 group IIA phospholipase A2 phosphatidylcholine 2-acylhydrolase 2A phospholipase A2, group IIA (platelets, synovial fluid) platelet phospholipase A2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	hydrolyzes acyl group at the sn-2 position of phospholipids, forming non-esterified fatty acids and lysophospholipids; plays a critical role in inflammation; induces proliferation of smooth muscle cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239146.2	XP_006239208.1	phospholipase A2, membrane associated isoform X1
NM_031598.3	NP_113786.3	phospholipase A2, membrane associated precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PLA2G2A	NP_001020495.1
Gallus gallus (chicken)	PLA2G2A	NP_001264843.1
Homo sapiens (human)	PLA2G2A	NP_000291.1
Bos taurus (cattle)	LOC615045	XP_002685775.1
Pan troglodytes (chimpanzee)	PLA2G2A	XP_001160344.1
Rattus norvegicus (Norway rat)	Pla2g2a	NP_113786.3
	PLA2G2A	XP_001094731.1

Gene Ontology
Bibliography

Related articles in PubMed

AMPK Signaling Involvement for the Repression of the IL-1?-Induced Group IIA Secretory Phospholipase A2 Expression in VSMCs.
El Hadri K, Denoyelle C, Ravaux L, Viollet B, Foretz M, Friguet B, Rouis M, Raymondjean M
PloS one10(7)e0132498(2015)

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

Regulatory effects of the JAK3/STAT1 pathway on the release of secreted phospholipase A?-IIA in microvascular endothelial cells of the injured brain.
Wang G, Qian P, Xu Z, Zhang J, Wang Y, Cheng S, Cai W, Qian G, Wang C, Decoster MA
Journal of neuroinflammation9170(2012 Jul)

Group V phospholipase A(2) increases pulmonary endothelial permeability through direct hydrolysis of the cell membrane.
Mu?oz NM, Desai A, Meliton LN, Meliton AY, Zhou T, Leff AR, Dudek SM
Pulmonary circulation2(2)182-92(2012 Apr-Jun)

Immunoaffinity purification, partial sequence, and subcellular localization of rat liver phospholipase A2.
Aarsman AJ, de Jong JG, Arnoldussen E, Neys FW, van Wassenaar PD, Van den Bosch H
The Journal of biological chemistry264(17)10008-14(1989 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study was designed to determine the relationship between phenformin coupling AMP-activated protein kinase (AMPK) function and the molecular mechanism by which the sPLA2 IIA expression was modulated in vascular smooth muscle cells.
Title: AMPK Signaling Involvement for the Repression of the IL-1?-Induced Group IIA Secretory Phospholipase A2 Expression in VSMCs.

a novel inverse recruitment mechanism in which liganded TRbeta recruits corepressors to inhibit PLA2g2a expression.
Title: Nuclear corepressors mediate the repression of phospholipase A2 group IIa gene transcription by thyroid hormone.

Secreted PLA2-IIA regulates the permeability of microvascular endothelial cells during brain inflammation.
Title: Regulatory effects of the JAK3/STAT1 pathway on the release of secreted phospholipase A?-IIA in microvascular endothelial cells of the injured brain.

cPLA(2)alpha-mediated induction of PPAR-beta/delta is a novel intracellular signalling pathway in spontaneous and TGF-beta induced activation of hepatic stellate cells.
Title: Involvement of cytosolic phospholipase A2 alpha signalling pathway in spontaneous and transforming growth factor-beta-induced activation of rat hepatic stellate cells.

Data show changes in cell morphology and upregulation of ERK1/2, iNOS and sPLA-IIA expression in astrocytes and microglia on exposure to lipopolysaccharides and cytokines.
Title: Pro-inflammatory cytokines and lipopolysaccharide induce changes in cell morphology, and upregulation of ERK1/2, iNOS and sPLA?-IIA expression in astrocytes and microglia.

The following Pla2g2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pla2g2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08217	NM_031598.3 Latest version!	Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa08217	XM_006239146.2 Latest version!	Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pla2g2a ( NM_031598.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113786.3 Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), mRNA.

Price & Availability↑

CloneID	ORa08217
Clone ID Related Accession (Same CDS sequence)	XM_006239146.2 , NM_031598.3
Accession Version	NM_031598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase A2, membrane associated precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M25148.1, X74364.1 and CB772812.1. On Mar 27, 2010 this sequence version replaced NM_031598.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ227448.1, D00523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689596 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCC TCCTGTTGCT AGCAGTTGTG ATCATGGCCT TTGGCTCAAT TCAGGTCCAG 
GGGAGCCTTC TGGAGTTTGG GCAAATGATT CTGTTTAAGA CAGGAAAGAG AGCTGATGTT 
AGCTATGGCT TCTACGGTTG CCATTGTGGT GTGGGTGGCA GAGGATCCCC CAAGGATGCC 
ACAGATTGGT GCTGTGTGAC TCATGACTGT TGTTACAACC GTCTGGAGAA ACGTGGATGT 
GGCACAAAGT TTCTGACCTA CAAGTTCTCC TACCGAGGGG GCCAAATCTC CTGCTCTACA 
AACCAGGACT CCTGCCGGAA ACAGCTGTGC CAGTGCGATA AAGCTGCCGC TGAATGTTTT 
GCCCGGAACA AGAAAAGCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAA GTTTTGCAAA 
GGGAAGACGC CCAGTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113786.3
CDS	58..498
Translation	MKVLLLLAVVIMAFGSIQVQGSLLEFGQMILFKTGKRADVSYGFYGCHCGVGGRGSPKDATDWCCVTHDCCYNRLEKRGCGTKFLTYKFSYRGGQISCSTNQDSCRKQLCQCDKAAAECFARNKKSYSLKYQFYPNKFCKGKTPSC

Target ORF information:

RefSeq Version	NM_031598.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031598.3

ORF Insert Sequence:

ATGAAGGTCC TCCTGTTGCT AGCAGTTGTG ATCATGGCCT TTGGCTCAAT TCAGGTCCAG 
GGGAGCCTTC TGGAGTTTGG GCAAATGATT CTGTTTAAGA CAGGAAAGAG AGCTGATGTT 
AGCTATGGCT TCTACGGTTG CCATTGTGGT GTGGGTGGCA GAGGATCCCC CAAGGATGCC 
ACAGATTGGT GCTGTGTGAC TCATGACTGT TGTTACAACC GTCTGGAGAA ACGTGGATGT 
GGCACAAAGT TTCTGACCTA CAAGTTCTCC TACCGAGGGG GCCAAATCTC CTGCTCTACA 
AACCAGGACT CCTGCCGGAA ACAGCTGTGC CAGTGCGATA AAGCTGCCGC TGAATGTTTT 
GCCCGGAACA AGAAAAGCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAA GTTTTGCAAA 
GGGAAGACGC CCAGTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pla2g2a ( XM_006239146.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239208.1 Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08217
Clone ID Related Accession (Same CDS sequence)	XM_006239146.2 , NM_031598.3
Accession Version	XM_006239146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase A2, membrane associated isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006239146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCC TCCTGTTGCT AGCAGTTGTG ATCATGGCCT TTGGCTCAAT TCAGGTCCAG 
GGGAGCCTTC TGGAGTTTGG GCAAATGATT CTGTTTAAGA CAGGAAAGAG AGCTGATGTT 
AGCTATGGCT TCTACGGTTG CCATTGTGGT GTGGGTGGCA GAGGATCCCC CAAGGATGCC 
ACAGATTGGT GCTGTGTGAC TCATGACTGT TGTTACAACC GTCTGGAGAA ACGTGGATGT 
GGCACAAAGT TTCTGACCTA CAAGTTCTCC TACCGAGGGG GCCAAATCTC CTGCTCTACA 
AACCAGGACT CCTGCCGGAA ACAGCTGTGC CAGTGCGATA AAGCTGCCGC TGAATGTTTT 
GCCCGGAACA AGAAAAGCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAA GTTTTGCAAA 
GGGAAGACGC CCAGTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239208.1
CDS	224..664
Translation	MKVLLLLAVVIMAFGSIQVQGSLLEFGQMILFKTGKRADVSYGFYGCHCGVGGRGSPKDATDWCCVTHDCCYNRLEKRGCGTKFLTYKFSYRGGQISCSTNQDSCRKQLCQCDKAAAECFARNKKSYSLKYQFYPNKFCKGKTPSC

Target ORF information:

RefSeq Version	XM_006239146.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2 group IIA (Pla2g2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239146.2

ORF Insert Sequence:

ATGAAGGTCC TCCTGTTGCT AGCAGTTGTG ATCATGGCCT TTGGCTCAAT TCAGGTCCAG 
GGGAGCCTTC TGGAGTTTGG GCAAATGATT CTGTTTAAGA CAGGAAAGAG AGCTGATGTT 
AGCTATGGCT TCTACGGTTG CCATTGTGGT GTGGGTGGCA GAGGATCCCC CAAGGATGCC 
ACAGATTGGT GCTGTGTGAC TCATGACTGT TGTTACAACC GTCTGGAGAA ACGTGGATGT 
GGCACAAAGT TTCTGACCTA CAAGTTCTCC TACCGAGGGG GCCAAATCTC CTGCTCTACA 
AACCAGGACT CCTGCCGGAA ACAGCTGTGC CAGTGCGATA AAGCTGCCGC TGAATGTTTT 
GCCCGGAACA AGAAAAGCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAA GTTTTGCAAA 
GGGAAGACGC CCAGTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AMPK Signaling Involvement for the Repression of the IL-1?-Induced Group IIA Secretory Phospholipase A2 Expression in VSMCs.
PloS one10(7)e0132498(2015)
El Hadri K,Denoyelle C,Ravaux L,Viollet B,Foretz M,Friguet B,Rouis M,Raymondjean M

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Molecular bioSystems9(6)1108-16(2013 Jun)
Pieragostino D,Agnifili L,Fasanella V,D'Aguanno S,Mastropasqua R,Di Ilio C,Sacchetta P,Urbani A,Del Boccio P

Regulatory effects of the JAK3/STAT1 pathway on the release of secreted phospholipase A?-IIA in microvascular endothelial cells of the injured brain.
Journal of neuroinflammation9170(2012 Jul)
Wang G,Qian P,Xu Z,Zhang J,Wang Y,Cheng S,Cai W,Qian G,Wang C,Decoster MA

Group V phospholipase A(2) increases pulmonary endothelial permeability through direct hydrolysis of the cell membrane.
Pulmonary circulation2(2)182-92(2012 Apr-Jun)
Mu?oz NM,Desai A,Meliton LN,Meliton AY,Zhou T,Leff AR,Dudek SM

Immunoaffinity purification, partial sequence, and subcellular localization of rat liver phospholipase A2.
The Journal of biological chemistry264(17)10008-14(1989 Jun)
Aarsman AJ,de Jong JG,Arnoldussen E,Neys FW,van Wassenaar PD,Van den Bosch H

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Pla2g2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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