Sema3e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema3e
Entrez Gene ID	296789
Full Name	semaphorin 3E
General protein information	Preferred Names semaphorin 3E Names semaphorin-3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106579.1	NP_001100049.1	semaphorin-3E precursor
XM_017592522.1	XP_017448011.1	semaphorin-3E isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEMA3E	NP_036563.1
Pan troglodytes (chimpanzee)	SEMA3E	XP_527803.3
Mus musculus (house mouse)	Sema3e	NP_035478.2
Rattus norvegicus (Norway rat)	Sema3e	NP_001100049.1
	SEMA3E	XP_002803437.1
Gallus gallus (chicken)	SEMA3E	NP_989573.1
Danio rerio (zebrafish)	sema3e	NP_001038549.1
Canis lupus familiaris (dog)	SEMA3E	XP_005631079.1
Bos taurus (cattle)	SEMA3E	XP_003586003.2
Xenopus tropicalis (tropical clawed frog)	sema3e	XP_002935302.1

Gene Ontology
Bibliography

Related articles in PubMed

An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling.
Tata A, Stoppel DC, Hong S, Ben-Zvi A, Xie T, Gu C
The Journal of cell biology205(4)573-90(2014 May)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Semaphorin 3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum.
Ding JB, Oh WJ, Sabatini BL, Gu C
Nature neuroscience15(2)215-23(2011 Dec)

Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras.
Sakurai A, Gavard J, Annas-Linhares Y, Basile JR, Amornphimoltham P, Palmby TR, Yagi H, Zhang F, Randazzo PA, Li X, Weigert R, Gutkind JS
Molecular and cellular biology30(12)3086-98(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sema3e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema3e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01685	NM_001106579.1 Latest version!	Rattus norvegicus semaphorin 3E (Sema3e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01685	XM_017592522.1 Latest version!	Rattus norvegicus semaphorin 3E (Sema3e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema3e ( NM_001106579.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100049.1 Rattus norvegicus semaphorin 3E (Sema3e), mRNA.

Price & Availability↑

CloneID	ORa01685
Clone ID Related Accession (Same CDS sequence)	NM_001106579.1 , XM_017592522.1
Accession Version	NM_001106579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-3E precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474020.1. On or before Oct 22, 2008 this sequence version replaced XM_231354.4, XM_001064828.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCACCGA CCGGACACAT CCTCACCTTG CTGCTCTGGG GTCACCTGCT GGAGCTCTGG 
ACCCCAGGTT ACACTGCGAA CCCCTCCTAT CCCAGGCTAC GACTGTCTCA TAAAGAACTC 
TTGGAACTGA ACAGGACGTC AATATTTCAA AGCCCCTTTG GTTTTCTTGA TCTCCACACA 
ATGCTGCTGG ATGAGTATCA AGAACGGCTC TTTGTGGGAG GCAGAGACCT TGTCTATTCC 
CTGAACTTGG AACGCGTCAG TGATGGCTAC AGAGAGATAT ACTGGCCTAG CACAGCAGTA 
AAAGTAGAAG AATGCATAAT GAAAGGAAAA GACGCAAATG AGTGTGCCAA TTATGTCCGG 
GTTTTGCATC ACTACAACAG GACACACCTT CTGACCTGTG CTACGGGAGC CTTTGATCCA 
CACTGTGCCT TTATCCGAGT CGGGCACCAT TCAGAGGACC CCCTGTTTCA CCTGGAGTCA 
CACAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAACTCCTC CTTTGTGTCC 
ACACTAGTTG GTAATGAACT GTTTGCTGGG CTCTACAGTG ACTACTGGGG CCGAGACTCA 
GCGATCTTCC GCAGCCTGGG GAAGCTCGGC CATATCCGCA CCGAGCATGA CGATGAGCGG 
CTGCTGAAAG AACCAAAATT TGTAGGTTCA TATATGATTC CTGATAACGA AGACCCAGAG 
GACAACAAAA TGTACTTCTT CTTCACTGAG AAGGCATTGG AGGCAGAGAA CAATGCCCAC 
ACGATCTACA CCCGAGTGGG GCGGCTGTGC GTGAATGACA TGGGAGGACA GAGAATCCTG 
GTGAATAAGT GGAGCACATT CCTCAAAGCA CGGCTGGTTT GCTCAGTACC AGGGATGAAT 
GGAATTGACA CACACTTTGA CGAATTAGAG GATGTGTTTT TACTGCCTAC CCGAGATCCC 
AAGAATCCAG TGATATTTGG ACTATTTAAT ACTACCAGCA ATATTTTTAG AGGCCATGCT 
GTATGTGTGT ATCACATGTC AAGTATCCGG GAAGCCTTTA ATGGCCCATA TGCACATAAA 
GAAGGCCCTG AATATCACTG GTCACTGTAT GAAGGAAAGG TCCCCTACCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTGAA TGGAGGAAAG TACGGATCCA CCAAAGACTA CCCTGACGAC 
GCCATCCGAT TCGCAAGGAT GCATCCACTA ATGTATCAGC CCATAAAACC TGTCCATAAA 
AAACCAATAC TGGTAAAAAC GGATGGAAAA TATAACCTGA GGCAACTAGC AGTGGACCGA 
GTGGAAGCGG AGGATGGCCA GTATGACGTT TTATTTATTG GAACAGACAC AGGAATCGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ACAGAGTGGA TGGAGGAAGT CATTCTGGAG 
GAACTTCAAA TATTCAAGGA TCCAGCCCCT ATCATTTCTA TGGAGATTTC TTCAAAGAGG 
CAACAGCTCT ATATTGGGTC AGCCTCTGCT GTGGCACAAG TCAGATTCCA TCACTGTGAC 
ATGTACGGCA GCGCCTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGTATCTCCT GCTCCAGGTA CTACCCGACA GGTGCACACT CAAAGAGGAG ATTCCGCAGG 
CAGGATGTTC GGCACGGCAA CGCCGCCCAG CAGTGCTTTG GGCAGCAATT TGTGGGAGAC 
GCTTTGGATA GGACTGAAGA GAGGCTGGCT TATGGCATAG AGAGCAACAG TACTTTGCTG 
GAATGCACCC CACGATCACT ACAAGCAAAA GTTGTCTGGT TTGTACAGAA GGGACGCGAC 
GTAAGAAAGG AAGAGGTAAA GACGGATGAC AGAGTTGTTA AGATGGACTT GGGCTTGCTC 
TTCCTCAGAG TCCGCAAGTC GGATGCAGGG ACCTACTTTT GCCAAACGGT AGAACATAGC 
TTCGTCCACA CTGTGCGCAA AATCACCTTG GAGGTGGTCG AAGAGCATAA AGTGGAGGGG 
ATGTTTCATA AGGACCGCGA AGAGGAAAAA CATCACAAGA TGCCCTGCCC TCCCTTAACT 
GGTATGTCTC AGGGGACAAA ACCCTGGTAC AAGGAATTCT TGCAGCTGAT TGGCTACAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAA AAGGTGTGGT GTACAGATAA GAAGAGGAAA 
AAGCTGAAAA TGTCTCCCTC CAAGTGGAAG TATGCCAACC CCCAGGAAAA GAGGCTTCGC 
TCCAAAGCTG AGCATTTCCG CCTGCCCAGG CACACGCTGG TCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100049.1
CDS	599..2926
Translation	MAPTGHILTLLLWGHLLELWTPGYTANPSYPRLRLSHKELLELNRTSIFQSPFGFLDLHTMLLDEYQERLFVGGRDLVYSLNLERVSDGYREIYWPSTAVKVEECIMKGKDANECANYVRVLHHYNRTHLLTCATGAFDPHCAFIRVGHHSEDPLFHLESHRSERGRGRCPFDPNSSFVSTLVGNELFAGLYSDYWGRDSAIFRSLGKLGHIRTEHDDERLLKEPKFVGSYMIPDNEDPEDNKMYFFFTEKALEAENNAHTIYTRVGRLCVNDMGGQRILVNKWSTFLKARLVCSVPGMNGIDTHFDELEDVFLLPTRDPKNPVIFGLFNTTSNIFRGHAVCVYHMSSIREAFNGPYAHKEGPEYHWSLYEGKVPYPRPGSCASKVNGGKYGSTKDYPDDAIRFARMHPLMYQPIKPVHKKPILVKTDGKYNLRQLAVDRVEAEDGQYDVLFIGTDTGIVLKVITIYNQETEWMEEVILEELQIFKDPAPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGAHSKRRFRRQDVRHGNAAQQCFGQQFVGDALDRTEERLAYGIESNSTLLECTPRSLQAKVVWFVQKGRDVRKEEVKTDDRVVKMDLGLLFLRVRKSDAGTYFCQTVEHSFVHTVRKITLEVVEEHKVEGMFHKDREEEKHHKMPCPPLTGMSQGTKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDKKRKKLKMSPSKWKYANPQEKRLRSKAEHFRLPRHTLVS

Target ORF information:

RefSeq Version	NM_001106579.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 3E (Sema3e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106579.1

ORF Insert Sequence:

ATGGCACCGA CCGGACACAT CCTCACCTTG CTGCTCTGGG GTCACCTGCT GGAGCTCTGG 
ACCCCAGGTT ACACTGCGAA CCCCTCCTAT CCCAGGCTAC GACTGTCTCA TAAAGAACTC 
TTGGAACTGA ACAGGACGTC AATATTTCAA AGCCCCTTTG GTTTTCTTGA TCTCCACACA 
ATGCTGCTGG ATGAGTATCA AGAACGGCTC TTTGTGGGAG GCAGAGACCT TGTCTATTCC 
CTGAACTTGG AACGCGTCAG TGATGGCTAC AGAGAGATAT ACTGGCCTAG CACAGCAGTA 
AAAGTAGAAG AATGCATAAT GAAAGGAAAA GACGCAAATG AGTGTGCCAA TTATGTCCGG 
GTTTTGCATC ACTACAACAG GACACACCTT CTGACCTGTG CTACGGGAGC CTTTGATCCA 
CACTGTGCCT TTATCCGAGT CGGGCACCAT TCAGAGGACC CCCTGTTTCA CCTGGAGTCA 
CACAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAACTCCTC CTTTGTGTCC 
ACACTAGTTG GTAATGAACT GTTTGCTGGG CTCTACAGTG ACTACTGGGG CCGAGACTCA 
GCGATCTTCC GCAGCCTGGG GAAGCTCGGC CATATCCGCA CCGAGCATGA CGATGAGCGG 
CTGCTGAAAG AACCAAAATT TGTAGGTTCA TATATGATTC CTGATAACGA AGACCCAGAG 
GACAACAAAA TGTACTTCTT CTTCACTGAG AAGGCATTGG AGGCAGAGAA CAATGCCCAC 
ACGATCTACA CCCGAGTGGG GCGGCTGTGC GTGAATGACA TGGGAGGACA GAGAATCCTG 
GTGAATAAGT GGAGCACATT CCTCAAAGCA CGGCTGGTTT GCTCAGTACC AGGGATGAAT 
GGAATTGACA CACACTTTGA CGAATTAGAG GATGTGTTTT TACTGCCTAC CCGAGATCCC 
AAGAATCCAG TGATATTTGG ACTATTTAAT ACTACCAGCA ATATTTTTAG AGGCCATGCT 
GTATGTGTGT ATCACATGTC AAGTATCCGG GAAGCCTTTA ATGGCCCATA TGCACATAAA 
GAAGGCCCTG AATATCACTG GTCACTGTAT GAAGGAAAGG TCCCCTACCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTGAA TGGAGGAAAG TACGGATCCA CCAAAGACTA CCCTGACGAC 
GCCATCCGAT TCGCAAGGAT GCATCCACTA ATGTATCAGC CCATAAAACC TGTCCATAAA 
AAACCAATAC TGGTAAAAAC GGATGGAAAA TATAACCTGA GGCAACTAGC AGTGGACCGA 
GTGGAAGCGG AGGATGGCCA GTATGACGTT TTATTTATTG GAACAGACAC AGGAATCGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ACAGAGTGGA TGGAGGAAGT CATTCTGGAG 
GAACTTCAAA TATTCAAGGA TCCAGCCCCT ATCATTTCTA TGGAGATTTC TTCAAAGAGG 
CAACAGCTCT ATATTGGGTC AGCCTCTGCT GTGGCACAAG TCAGATTCCA TCACTGTGAC 
ATGTACGGCA GCGCCTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGTATCTCCT GCTCCAGGTA CTACCCGACA GGTGCACACT CAAAGAGGAG ATTCCGCAGG 
CAGGATGTTC GGCACGGCAA CGCCGCCCAG CAGTGCTTTG GGCAGCAATT TGTGGGAGAC 
GCTTTGGATA GGACTGAAGA GAGGCTGGCT TATGGCATAG AGAGCAACAG TACTTTGCTG 
GAATGCACCC CACGATCACT ACAAGCAAAA GTTGTCTGGT TTGTACAGAA GGGACGCGAC 
GTAAGAAAGG AAGAGGTAAA GACGGATGAC AGAGTTGTTA AGATGGACTT GGGCTTGCTC 
TTCCTCAGAG TCCGCAAGTC GGATGCAGGG ACCTACTTTT GCCAAACGGT AGAACATAGC 
TTCGTCCACA CTGTGCGCAA AATCACCTTG GAGGTGGTCG AAGAGCATAA AGTGGAGGGG 
ATGTTTCATA AGGACCGCGA AGAGGAAAAA CATCACAAGA TGCCCTGCCC TCCCTTAACT 
GGTATGTCTC AGGGGACAAA ACCCTGGTAC AAGGAATTCT TGCAGCTGAT TGGCTACAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAA AAGGTGTGGT GTACAGATAA GAAGAGGAAA 
AAGCTGAAAA TGTCTCCCTC CAAGTGGAAG TATGCCAACC CCCAGGAAAA GAGGCTTCGC 
TCCAAAGCTG AGCATTTCCG CCTGCCCAGG CACACGCTGG TCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema3e ( XM_017592522.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448011.1 Rattus norvegicus semaphorin 3E (Sema3e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01685
Clone ID Related Accession (Same CDS sequence)	NM_001106579.1 , XM_017592522.1
Accession Version	XM_017592522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-3E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCGA CCGGACACAT CCTCACCTTG CTGCTCTGGG GTCACCTGCT GGAGCTCTGG 
ACCCCAGGTT ACACTGCGAA CCCCTCCTAT CCCAGGCTAC GACTGTCTCA TAAAGAACTC 
TTGGAACTGA ACAGGACGTC AATATTTCAA AGCCCCTTTG GTTTTCTTGA TCTCCACACA 
ATGCTGCTGG ATGAGTATCA AGAACGGCTC TTTGTGGGAG GCAGAGACCT TGTCTATTCC 
CTGAACTTGG AACGCGTCAG TGATGGCTAC AGAGAGATAT ACTGGCCTAG CACAGCAGTA 
AAAGTAGAAG AATGCATAAT GAAAGGAAAA GACGCAAATG AGTGTGCCAA TTATGTCCGG 
GTTTTGCATC ACTACAACAG GACACACCTT CTGACCTGTG CTACGGGAGC CTTTGATCCA 
CACTGTGCCT TTATCCGAGT CGGGCACCAT TCAGAGGACC CCCTGTTTCA CCTGGAGTCA 
CACAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAACTCCTC CTTTGTGTCC 
ACACTAGTTG GTAATGAACT GTTTGCTGGG CTCTACAGTG ACTACTGGGG CCGAGACTCA 
GCGATCTTCC GCAGCCTGGG GAAGCTCGGC CATATCCGCA CCGAGCATGA CGATGAGCGG 
CTGCTGAAAG AACCAAAATT TGTAGGTTCA TATATGATTC CTGATAACGA AGACCCAGAG 
GACAACAAAA TGTACTTCTT CTTCACTGAG AAGGCATTGG AGGCAGAGAA CAATGCCCAC 
ACGATCTACA CCCGAGTGGG GCGGCTGTGC GTGAATGACA TGGGAGGACA GAGAATCCTG 
GTGAATAAGT GGAGCACATT CCTCAAAGCA CGGCTGGTTT GCTCAGTACC AGGGATGAAT 
GGAATTGACA CACACTTTGA CGAATTAGAG GATGTGTTTT TACTGCCTAC CCGAGATCCC 
AAGAATCCAG TGATATTTGG ACTATTTAAT ACTACCAGCA ATATTTTTAG AGGCCATGCT 
GTATGTGTGT ATCACATGTC AAGTATCCGG GAAGCCTTTA ATGGCCCATA TGCACATAAA 
GAAGGCCCTG AATATCACTG GTCACTGTAT GAAGGAAAGG TCCCCTACCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTGAA TGGAGGAAAG TACGGATCCA CCAAAGACTA CCCTGACGAC 
GCCATCCGAT TCGCAAGGAT GCATCCACTA ATGTATCAGC CCATAAAACC TGTCCATAAA 
AAACCAATAC TGGTAAAAAC GGATGGAAAA TATAACCTGA GGCAACTAGC AGTGGACCGA 
GTGGAAGCGG AGGATGGCCA GTATGACGTT TTATTTATTG GAACAGACAC AGGAATCGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ACAGAGTGGA TGGAGGAAGT CATTCTGGAG 
GAACTTCAAA TATTCAAGGA TCCAGCCCCT ATCATTTCTA TGGAGATTTC TTCAAAGAGG 
CAACAGCTCT ATATTGGGTC AGCCTCTGCT GTGGCACAAG TCAGATTCCA TCACTGTGAC 
ATGTACGGCA GCGCCTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGTATCTCCT GCTCCAGGTA CTACCCGACA GGTGCACACT CAAAGAGGAG ATTCCGCAGG 
CAGGATGTTC GGCACGGCAA CGCCGCCCAG CAGTGCTTTG GGCAGCAATT TGTGGGAGAC 
GCTTTGGATA GGACTGAAGA GAGGCTGGCT TATGGCATAG AGAGCAACAG TACTTTGCTG 
GAATGCACCC CACGATCACT ACAAGCAAAA GTTGTCTGGT TTGTACAGAA GGGACGCGAC 
GTAAGAAAGG AAGAGGTAAA GACGGATGAC AGAGTTGTTA AGATGGACTT GGGCTTGCTC 
TTCCTCAGAG TCCGCAAGTC GGATGCAGGG ACCTACTTTT GCCAAACGGT AGAACATAGC 
TTCGTCCACA CTGTGCGCAA AATCACCTTG GAGGTGGTCG AAGAGCATAA AGTGGAGGGG 
ATGTTTCATA AGGACCGCGA AGAGGAAAAA CATCACAAGA TGCCCTGCCC TCCCTTAACT 
GGTATGTCTC AGGGGACAAA ACCCTGGTAC AAGGAATTCT TGCAGCTGAT TGGCTACAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAA AAGGTGTGGT GTACAGATAA GAAGAGGAAA 
AAGCTGAAAA TGTCTCCCTC CAAGTGGAAG TATGCCAACC CCCAGGAAAA GAGGCTTCGC 
TCCAAAGCTG AGCATTTCCG CCTGCCCAGG CACACGCTGG TCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448011.1
CDS	584..2911
Translation	MAPTGHILTLLLWGHLLELWTPGYTANPSYPRLRLSHKELLELNRTSIFQSPFGFLDLHTMLLDEYQERLFVGGRDLVYSLNLERVSDGYREIYWPSTAVKVEECIMKGKDANECANYVRVLHHYNRTHLLTCATGAFDPHCAFIRVGHHSEDPLFHLESHRSERGRGRCPFDPNSSFVSTLVGNELFAGLYSDYWGRDSAIFRSLGKLGHIRTEHDDERLLKEPKFVGSYMIPDNEDPEDNKMYFFFTEKALEAENNAHTIYTRVGRLCVNDMGGQRILVNKWSTFLKARLVCSVPGMNGIDTHFDELEDVFLLPTRDPKNPVIFGLFNTTSNIFRGHAVCVYHMSSIREAFNGPYAHKEGPEYHWSLYEGKVPYPRPGSCASKVNGGKYGSTKDYPDDAIRFARMHPLMYQPIKPVHKKPILVKTDGKYNLRQLAVDRVEAEDGQYDVLFIGTDTGIVLKVITIYNQETEWMEEVILEELQIFKDPAPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGAHSKRRFRRQDVRHGNAAQQCFGQQFVGDALDRTEERLAYGIESNSTLLECTPRSLQAKVVWFVQKGRDVRKEEVKTDDRVVKMDLGLLFLRVRKSDAGTYFCQTVEHSFVHTVRKITLEVVEEHKVEGMFHKDREEEKHHKMPCPPLTGMSQGTKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDKKRKKLKMSPSKWKYANPQEKRLRSKAEHFRLPRHTLVS

Target ORF information:

RefSeq Version	XM_017592522.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 3E (Sema3e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592522.1

ORF Insert Sequence:

ATGGCACCGA CCGGACACAT CCTCACCTTG CTGCTCTGGG GTCACCTGCT GGAGCTCTGG 
ACCCCAGGTT ACACTGCGAA CCCCTCCTAT CCCAGGCTAC GACTGTCTCA TAAAGAACTC 
TTGGAACTGA ACAGGACGTC AATATTTCAA AGCCCCTTTG GTTTTCTTGA TCTCCACACA 
ATGCTGCTGG ATGAGTATCA AGAACGGCTC TTTGTGGGAG GCAGAGACCT TGTCTATTCC 
CTGAACTTGG AACGCGTCAG TGATGGCTAC AGAGAGATAT ACTGGCCTAG CACAGCAGTA 
AAAGTAGAAG AATGCATAAT GAAAGGAAAA GACGCAAATG AGTGTGCCAA TTATGTCCGG 
GTTTTGCATC ACTACAACAG GACACACCTT CTGACCTGTG CTACGGGAGC CTTTGATCCA 
CACTGTGCCT TTATCCGAGT CGGGCACCAT TCAGAGGACC CCCTGTTTCA CCTGGAGTCA 
CACAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAACTCCTC CTTTGTGTCC 
ACACTAGTTG GTAATGAACT GTTTGCTGGG CTCTACAGTG ACTACTGGGG CCGAGACTCA 
GCGATCTTCC GCAGCCTGGG GAAGCTCGGC CATATCCGCA CCGAGCATGA CGATGAGCGG 
CTGCTGAAAG AACCAAAATT TGTAGGTTCA TATATGATTC CTGATAACGA AGACCCAGAG 
GACAACAAAA TGTACTTCTT CTTCACTGAG AAGGCATTGG AGGCAGAGAA CAATGCCCAC 
ACGATCTACA CCCGAGTGGG GCGGCTGTGC GTGAATGACA TGGGAGGACA GAGAATCCTG 
GTGAATAAGT GGAGCACATT CCTCAAAGCA CGGCTGGTTT GCTCAGTACC AGGGATGAAT 
GGAATTGACA CACACTTTGA CGAATTAGAG GATGTGTTTT TACTGCCTAC CCGAGATCCC 
AAGAATCCAG TGATATTTGG ACTATTTAAT ACTACCAGCA ATATTTTTAG AGGCCATGCT 
GTATGTGTGT ATCACATGTC AAGTATCCGG GAAGCCTTTA ATGGCCCATA TGCACATAAA 
GAAGGCCCTG AATATCACTG GTCACTGTAT GAAGGAAAGG TCCCCTACCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTGAA TGGAGGAAAG TACGGATCCA CCAAAGACTA CCCTGACGAC 
GCCATCCGAT TCGCAAGGAT GCATCCACTA ATGTATCAGC CCATAAAACC TGTCCATAAA 
AAACCAATAC TGGTAAAAAC GGATGGAAAA TATAACCTGA GGCAACTAGC AGTGGACCGA 
GTGGAAGCGG AGGATGGCCA GTATGACGTT TTATTTATTG GAACAGACAC AGGAATCGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ACAGAGTGGA TGGAGGAAGT CATTCTGGAG 
GAACTTCAAA TATTCAAGGA TCCAGCCCCT ATCATTTCTA TGGAGATTTC TTCAAAGAGG 
CAACAGCTCT ATATTGGGTC AGCCTCTGCT GTGGCACAAG TCAGATTCCA TCACTGTGAC 
ATGTACGGCA GCGCCTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGTATCTCCT GCTCCAGGTA CTACCCGACA GGTGCACACT CAAAGAGGAG ATTCCGCAGG 
CAGGATGTTC GGCACGGCAA CGCCGCCCAG CAGTGCTTTG GGCAGCAATT TGTGGGAGAC 
GCTTTGGATA GGACTGAAGA GAGGCTGGCT TATGGCATAG AGAGCAACAG TACTTTGCTG 
GAATGCACCC CACGATCACT ACAAGCAAAA GTTGTCTGGT TTGTACAGAA GGGACGCGAC 
GTAAGAAAGG AAGAGGTAAA GACGGATGAC AGAGTTGTTA AGATGGACTT GGGCTTGCTC 
TTCCTCAGAG TCCGCAAGTC GGATGCAGGG ACCTACTTTT GCCAAACGGT AGAACATAGC 
TTCGTCCACA CTGTGCGCAA AATCACCTTG GAGGTGGTCG AAGAGCATAA AGTGGAGGGG 
ATGTTTCATA AGGACCGCGA AGAGGAAAAA CATCACAAGA TGCCCTGCCC TCCCTTAACT 
GGTATGTCTC AGGGGACAAA ACCCTGGTAC AAGGAATTCT TGCAGCTGAT TGGCTACAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAA AAGGTGTGGT GTACAGATAA GAAGAGGAAA 
AAGCTGAAAA TGTCTCCCTC CAAGTGGAAG TATGCCAACC CCCAGGAAAA GAGGCTTCGC 
TCCAAAGCTG AGCATTTCCG CCTGCCCAGG CACACGCTGG TCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling.
The Journal of cell biology205(4)573-90(2014 May)
Tata A,Stoppel DC,Hong S,Ben-Zvi A,Xie T,Gu C

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Semaphorin 3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum.
Nature neuroscience15(2)215-23(2011 Dec)
Ding JB,Oh WJ,Sabatini BL,Gu C

Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras.
Molecular and cellular biology30(12)3086-98(2010 Jun)
Sakurai A,Gavard J,Annas-Linhares Y,Basile JR,Amornphimoltham P,Palmby TR,Yagi H,Zhang F,Randazzo PA,Li X,Weigert R,Gutkind JS

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Sema3e cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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