Ap1b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap1b1
Entrez Gene ID	29663
Full Name	adaptor-related protein complex 1, beta 1 subunit
Synonyms	Adtb1
General protein information	Preferred Names adaptor-related protein complex 1, beta 1 subunit Names AP-1 complex subunit beta-1 adaptor-related protein complex AP-1 beta 1 subunit beta-adaptin 1 clathrin assembly protein complex 1 beta large chain golgi adaptor HA1/AP1 adaptin beta subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	may direct receptors for inclusion in clathrin coated vesicles [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308298.1	NP_001295227.1	AP-1 complex subunit beta-1
NM_017277.1	NP_058973.1	AP-1 complex subunit beta-1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ap1b1	NP_058973.1
Gallus gallus (chicken)	AP1B1	XP_004934525.1
	AP1B1	XP_001106103.1
Canis lupus familiaris (dog)	AP1B1	XP_005636473.1
Mus musculus (house mouse)	Ap1b1	NP_001229973.1
Caenorhabditis elegans (roundworm)	apb-1	NP_001022937.1
Arabidopsis thaliana (thale cress)	AT4G23460	NP_194077.1
Homo sapiens (human)	AP1B1	NP_001118.3
Arabidopsis thaliana (thale cress)	AT4G11380	NP_192877.1
Oryza sativa (rice)	Os03g0355600	NP_001050135.1
Xenopus tropicalis (tropical clawed frog)	ap1b1	NP_001121444.1
Pan troglodytes (chimpanzee)	AP1B1	XP_003317209.1
Danio rerio (zebrafish)	ap1b1	NP_001122002.1
Drosophila melanogaster (fruit fly)	Bap	NP_523415.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001267	XP_321886.5
Bos taurus (cattle)	AP1B1	NP_001039403.2

Gene Ontology
Bibliography

Related articles in PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
Piccoli G, Condliffe SB, Bauer M, Giesert F, Boldt K, De Astis S, Meixner A, Sarioglu H, Vogt-Weisenhorn DM, Wurst W, Gloeckner CJ, Matteoli M, Sala C, Ueffing M
The Journal of neuroscience : the official journal of the Society for Neuroscience31(6)2225-37(2011 Feb)

Alternate pathways preserve tumor necrosis factor-alpha production after nuclear factor-kappaB inhibition in neonatal cerebral hypoxia-ischemia.
Nijboer CH, Heijnen CJ, Groenendaal F, van Bel F, Kavelaars A
Stroke40(10)3362-8(2009 Oct)

Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney.
Jung KJ, Lee EK, Kim JY, Zou Y, Sung B, Heo HS, Kim MK, Lee J, Kim ND, Yu BP, Chung HY
Inflammation research : official journal of the European Histamine Research Society ... [et al.]58(3)143-50(2009 Mar)

Matrix metalloproteinase-3 induction in rat brain astrocytes: focus on the role of two AP-1 elements.
Kim KS, Kim HY, Joe EH, Jou I
The Biochemical journal410(3)605-11(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data indicate that the switch to JNK/AP-1 activation preserves HI-induced TNF-alpha expression and thereby might contribute to the neuroprotective effect of TAT-NBD possibly through a TNF-R2 dependent mechanism.
Title: Alternate pathways preserve tumor necrosis factor-alpha production after nuclear factor-kappaB inhibition in neonatal cerebral hypoxia-ischemia.

Results suggest that 10-day calorie restriction can attenuate the altered signal transduction for inflammatory processes which is mediated through reactive species-induced NF-kB and AP-1 in aged kidney.
Title: Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney.

results indicate that AP-1 could control MMP-3 induction in brain astrocytes and that its regulation through specific AP-1 elements could be exploited in the treatment of brain pathologies in which increased expression of MMP-3 plays crucial roles
Title: Matrix metalloproteinase-3 induction in rat brain astrocytes: focus on the role of two AP-1 elements.

Ramiprilat exerts direct, bradykinin-receptor independent effects on cardiomyocytes that improve cellular function without a corresponding effect on AP-1 activation or induction of AP-1 dependent effects.
Title: Direct effects of the angiotensin-converting enzyme inhibitor ramiprilat on adult rat ventricular cardiomyocytes.

The following Ap1b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap1b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03514	NM_017277.1 Latest version!	Rattus norvegicus adaptor-related protein complex 1, beta 1 subunit (Ap1b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa43339	NM_001308298.1 Latest version!	Rattus norvegicus adaptor related protein complex 1 subunit beta 1 (Ap1b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap1b1 ( NM_017277.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058973.1 Rattus norvegicus adaptor-related protein complex 1, beta 1 subunit (Ap1b1), mRNA.

Price & Availability↑

CloneID	ORa03514
Clone ID Related Accession (Same CDS sequence)	NM_017277.1
Accession Version	NM_017277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	AP-1 complex subunit beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M77245.1. ##Evidence-Data-START## Transcript exon combination :: M77245.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACTGACT CAAAATACTT CACAACAACC AAGAAAGGGG AGATCTTCGA GTTGAAGGCA 
GAGCTTAACA GTGACAAGAA GGAGAAGAAG AAGGAGGCAG TAAAGAAAGT GATTGCATCA 
ATGACCGTGG GCAAAGATGT CAGTGCTCTC TTCCCTGATG TGGTGAACTG CATGCAGACA 
GACAACCTGG AGCTAAAGAA GCTGGTGTAC CTGTACTTGA TGAACTATGC CAAGAGTCAG 
CCTGACATGG CCATTATGGC TGTCAACACC TTCGTGAAGG ACTGTGAGGA CCCCAACCCC 
CTTATCCGGG CCCTGGCTGT GAGGACTATG GGCTGCATCC GAGTTGACAA GATCACAGAG 
TACTTGTGTG AACCACTCCG GAAATGCCTG AAGGATGAAG ACCCATATGT GCGCAAGACA 
GCAGCTGTGT GTGTAGCCAA GCTCCACGAC ATCAACGCCC AGCTGGTAGA GGACCAGGGT 
TTCCTGGACA CACTCAAAGA CCTCATCTCT GACTCTAACC CCATGGTGGT AGCAAATCGA 
GTGGCTGCCC TGTCAGAGAT CGCTGAGTCT CACCCCAGTA GCAACCTGCT TGACTTGAAG 
GCACAGTCTA TCAACAAGCT GCTGACGGCC CTTAATGAGT GCACCGAGTG GGCCCAGATC 
TTCATTCTGG ACTGTCTGGG CAACTACATG CCCAAGGATG ACCGTGAGGC CCAGAGCATC 
TGCGAGCGGG TCACTCCTAG GCTTTCCCAT GCCAACTCTG CTGTGGTGCT GTCCGCAGTG 
AAGGTCCTGA TGAAGTTCAT GGAGATGCTG TCTAAGGACC TGGACTACTA TGCCACACTA 
CTCAAGAAGC TGGCCCCTCC CCTGGTCACC CTGCTGTCAG CAGAGCCAGA GCCGCAGTAT 
GTGCCTCTGC GCAATATTAA CCTCATTGTA CAGAAAAGGC CAGAGATTTT GAAGCATGAA 
ATGAAGGTTT TCTTTGTGAA GTACAATGAC CCCATCTATG TGAAGCTGGA GAAACTGGAC 
ATTATGATCC GCCTGGCCTC CCAGGCCAAC ATTGCCCAGG TTTTGGCAGA ACTCAAAGAG 
TACGCGACAG AAGTAGATGT GGATTTTGTA CGGAAGGCAG TGCGTGCCAT TGGCCGCTGT 
GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CATTGCTTGA TCTCATCCAG 
ACCAAGGTCA ACTATGTGGT CCAGGAAGCC ATTGTGGTCA TTAAAGACAT CTTCCGCAAG 
TACCCCAACA AGTACGAGAG TGTGATAGCC ACACTGTGTG AGAATCTGGA CTCGCTGGAT 
GAGCCTGAGG CTCGCGCTGC TATGATCTGG ATAGTGGGCG AGTATGCTGA GCGGATTGAC 
AATGCGGACG AGCTGCTTGA GAGCTTCCTC GACGGCTTCC ATGACGAGAG CACCCAGGTC 
CAGCTACAGC TGCTGACAGC CATTGTGAAG CTCTTCCTGA AGAAGCCAAC AGAGACGCAG 
GAGCTGGTGC AGCAGGTCCT CAGTTTGGCC ACTCAGGACT CGGACAATCC AGACCTTCGG 
GACCGTGGCT ACATCTACTG GCGCCTGCTC TCCACGGACC CTGTAGCTGC CAAAGAGGTG 
GTACTGGCTG AGAAGCCCCT CATCTCAGAA GAGACAGACC TTATTGAGCC CACACTGCTG 
GATGAACTCA TCTGCTACAT TGGCACACTG GCCTCTGTCT ACCACAAGCC ACCCAATGCC 
TTTGTGGAGG GCGGCCGGGG AGTGGTGCAC AAGAGTCTGC CACCTCGCAC TGCCTCGAGC 
GAAAGCACAG AGAGCCCTGA GGCAGCCCCT GCCGGAGCAC CTGCTAGTGA CCAGCCTGAT 
GTCATTCCTG CCCAGGGTGA CCTGTTAGGT GACCTCCTCA ACCTGGACCT TGGGCCCCCA 
GTGAGTGGCC CACCCTTGGC TGCCTCCTCA GTGCAGATGG GAGCTGTGGA TCTTCTTGGT 
GGTGGCCTCG ACAGCCTGAT GGGGGATGAG CCTGAAGGGA TTGGAGACAG CAACTTTGGG 
GCACCTCCAG CATCAGTGGC GGCGGCAGCA CCAGCCAGAC TTGGAGCACC CGTCAGCAGT 
GGCTTAAGTG ACCTCTTTGA CCTGACCAGT GGAGTGGGCA CCCTCTCAGG GTCCTATGTC 
GCTCCCAAAG CAGTTTGGCT CCCTGCCATG AAGGCCAAGG GGCTGGAAAT CTCTGGCACC 
TTCACCCGGC AGGTTGGCTC CATCTCCATG GATCTGCAGC TGACTAACAA GGCCCTGCAG 
GTCATGACAG ATTTCGCTAT CCAGTTTAAT CGGAACAGCT TTGGCTTGGC CCCCGCTGCG 
CCACTCCAAG TGCACGCCCC ACTGAGCCCC AACCAGACGG TGGAGATCTC CCTGCCTCTT 
AACACGGTGG GCTCAGTCAT GAAGATGGAG CCTCTGAACA ATCTTCAGGT GGCTGTGAAG 
AACAACATTG ACGTCTTCTA CTTCAGCACT TTGTACCCAC TGCATGTCCT CTTCGTGGAG 
GACGGGAAGA TGGACCGGCA GATGTTCCTG GCCACATGGA AGGACATTCC CAACGAGAAT 
GAAGCCCAGT TCCAGATCAG AGACTGCCCG CTGAACACAG AGGCTGCAAG CAGCAAGCTG 
CAAAGCAGTA ACATTTTCAC TGTCGCCAAG AGGACTGTGG AGGGCCAGGA CATGCTCTAC 
CAATCTCTGA AGTTGACCAA TGGCATCTGG GTGCTGGCAG AGCTGCGGAT CCAGCCAGGC 
AACCCCAGTT TCACGCTGTC CCTGAAGTGT CGAGCCCCAG AGGTGTCCCA GCATGATATC 
CAGGCCTACG AGACCATCCT CAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058973.1
CDS	40..2889
Translation	MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANRVAALSEIAESHPSSNLLDLKAQSINKLLTALNECTEWAQIFILDCLGNYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYATLLKKLAPPLVTLLSAEPEPQYVPLRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLDGFHDESTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPNAFVEGGRGVVHKSLPPRTASSESTESPEAAPAGAPASDQPDVIPAQGDLLGDLLNLDLGPPVSGPPLAASSVQMGAVDLLGGGLDSLMGDEPEGIGDSNFGAPPASVAAAAPARLGAPVSSGLSDLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLNTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHVLFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNTEAASSKLQSSNIFTVAKRTVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSFTLSLKCRAPEVSQHDIQAYETILKN

Target ORF information:

RefSeq Version	NM_017277.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor-related protein complex 1, beta 1 subunit (Ap1b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017277.1

ORF Insert Sequence:

ATGACTGACT CAAAATACTT CACAACAACC AAGAAAGGGG AGATCTTCGA GTTGAAGGCA 
GAGCTTAACA GTGACAAGAA GGAGAAGAAG AAGGAGGCAG TAAAGAAAGT GATTGCATCA 
ATGACCGTGG GCAAAGATGT CAGTGCTCTC TTCCCTGATG TGGTGAACTG CATGCAGACA 
GACAACCTGG AGCTAAAGAA GCTGGTGTAC CTGTACTTGA TGAACTATGC CAAGAGTCAG 
CCTGACATGG CCATTATGGC TGTCAACACC TTCGTGAAGG ACTGTGAGGA CCCCAACCCC 
CTTATCCGGG CCCTGGCTGT GAGGACTATG GGCTGCATCC GAGTTGACAA GATCACAGAG 
TACTTGTGTG AACCACTCCG GAAATGCCTG AAGGATGAAG ACCCATATGT GCGCAAGACA 
GCAGCTGTGT GTGTAGCCAA GCTCCACGAC ATCAACGCCC AGCTGGTAGA GGACCAGGGT 
TTCCTGGACA CACTCAAAGA CCTCATCTCT GACTCTAACC CCATGGTGGT AGCAAATCGA 
GTGGCTGCCC TGTCAGAGAT CGCTGAGTCT CACCCCAGTA GCAACCTGCT TGACTTGAAG 
GCACAGTCTA TCAACAAGCT GCTGACGGCC CTTAATGAGT GCACCGAGTG GGCCCAGATC 
TTCATTCTGG ACTGTCTGGG CAACTACATG CCCAAGGATG ACCGTGAGGC CCAGAGCATC 
TGCGAGCGGG TCACTCCTAG GCTTTCCCAT GCCAACTCTG CTGTGGTGCT GTCCGCAGTG 
AAGGTCCTGA TGAAGTTCAT GGAGATGCTG TCTAAGGACC TGGACTACTA TGCCACACTA 
CTCAAGAAGC TGGCCCCTCC CCTGGTCACC CTGCTGTCAG CAGAGCCAGA GCCGCAGTAT 
GTGCCTCTGC GCAATATTAA CCTCATTGTA CAGAAAAGGC CAGAGATTTT GAAGCATGAA 
ATGAAGGTTT TCTTTGTGAA GTACAATGAC CCCATCTATG TGAAGCTGGA GAAACTGGAC 
ATTATGATCC GCCTGGCCTC CCAGGCCAAC ATTGCCCAGG TTTTGGCAGA ACTCAAAGAG 
TACGCGACAG AAGTAGATGT GGATTTTGTA CGGAAGGCAG TGCGTGCCAT TGGCCGCTGT 
GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CATTGCTTGA TCTCATCCAG 
ACCAAGGTCA ACTATGTGGT CCAGGAAGCC ATTGTGGTCA TTAAAGACAT CTTCCGCAAG 
TACCCCAACA AGTACGAGAG TGTGATAGCC ACACTGTGTG AGAATCTGGA CTCGCTGGAT 
GAGCCTGAGG CTCGCGCTGC TATGATCTGG ATAGTGGGCG AGTATGCTGA GCGGATTGAC 
AATGCGGACG AGCTGCTTGA GAGCTTCCTC GACGGCTTCC ATGACGAGAG CACCCAGGTC 
CAGCTACAGC TGCTGACAGC CATTGTGAAG CTCTTCCTGA AGAAGCCAAC AGAGACGCAG 
GAGCTGGTGC AGCAGGTCCT CAGTTTGGCC ACTCAGGACT CGGACAATCC AGACCTTCGG 
GACCGTGGCT ACATCTACTG GCGCCTGCTC TCCACGGACC CTGTAGCTGC CAAAGAGGTG 
GTACTGGCTG AGAAGCCCCT CATCTCAGAA GAGACAGACC TTATTGAGCC CACACTGCTG 
GATGAACTCA TCTGCTACAT TGGCACACTG GCCTCTGTCT ACCACAAGCC ACCCAATGCC 
TTTGTGGAGG GCGGCCGGGG AGTGGTGCAC AAGAGTCTGC CACCTCGCAC TGCCTCGAGC 
GAAAGCACAG AGAGCCCTGA GGCAGCCCCT GCCGGAGCAC CTGCTAGTGA CCAGCCTGAT 
GTCATTCCTG CCCAGGGTGA CCTGTTAGGT GACCTCCTCA ACCTGGACCT TGGGCCCCCA 
GTGAGTGGCC CACCCTTGGC TGCCTCCTCA GTGCAGATGG GAGCTGTGGA TCTTCTTGGT 
GGTGGCCTCG ACAGCCTGAT GGGGGATGAG CCTGAAGGGA TTGGAGACAG CAACTTTGGG 
GCACCTCCAG CATCAGTGGC GGCGGCAGCA CCAGCCAGAC TTGGAGCACC CGTCAGCAGT 
GGCTTAAGTG ACCTCTTTGA CCTGACCAGT GGAGTGGGCA CCCTCTCAGG GTCCTATGTC 
GCTCCCAAAG CAGTTTGGCT CCCTGCCATG AAGGCCAAGG GGCTGGAAAT CTCTGGCACC 
TTCACCCGGC AGGTTGGCTC CATCTCCATG GATCTGCAGC TGACTAACAA GGCCCTGCAG 
GTCATGACAG ATTTCGCTAT CCAGTTTAAT CGGAACAGCT TTGGCTTGGC CCCCGCTGCG 
CCACTCCAAG TGCACGCCCC ACTGAGCCCC AACCAGACGG TGGAGATCTC CCTGCCTCTT 
AACACGGTGG GCTCAGTCAT GAAGATGGAG CCTCTGAACA ATCTTCAGGT GGCTGTGAAG 
AACAACATTG ACGTCTTCTA CTTCAGCACT TTGTACCCAC TGCATGTCCT CTTCGTGGAG 
GACGGGAAGA TGGACCGGCA GATGTTCCTG GCCACATGGA AGGACATTCC CAACGAGAAT 
GAAGCCCAGT TCCAGATCAG AGACTGCCCG CTGAACACAG AGGCTGCAAG CAGCAAGCTG 
CAAAGCAGTA ACATTTTCAC TGTCGCCAAG AGGACTGTGG AGGGCCAGGA CATGCTCTAC 
CAATCTCTGA AGTTGACCAA TGGCATCTGG GTGCTGGCAG AGCTGCGGAT CCAGCCAGGC 
AACCCCAGTT TCACGCTGTC CCTGAAGTGT CGAGCCCCAG AGGTGTCCCA GCATGATATC 
CAGGCCTACG AGACCATCCT CAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap1b1 ( NM_001308298.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001295227.1 Rattus norvegicus adaptor related protein complex 1 subunit beta 1 (Ap1b1), mRNA.

Price & Availability↑

CloneID	ORa43339
Clone ID Related Accession (Same CDS sequence)	NM_001308298.1
Accession Version	NM_001308298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	AP-1 complex subunit beta-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07015907.1. On Jan 18, 2018 this sequence version replaced NM_017277.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: M77245.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTGACT CAAAATACTT CACAACAACC AAGAAAGGGG AGATCTTCGA GTTGAAGGCA 
GAGCTTAACA GTGACAAGAA GGAGAAGAAG AAGGAGGCAG TAAAGAAAGT GATTGCATCA 
ATGACCGTGG GCAAAGATGT CAGTGCTCTC TTCCCTGATG TGGTGAACTG CATGCAGACA 
GACAACCTGG AGCTAAAGAA GCTGGTGTAC CTGTACTTGA TGAACTATGC CAAGAGTCAG 
CCTGACATGG CCATTATGGC TGTCAACACC TTCGTGAAGG ACTGTGAGGA CCCCAACCCC 
CTTATCCGGG CCCTGGCTGT GAGGACTATG GGCTGCATCC GAGTTGACAA GATCACAGAG 
TACTTGTGTG AACCACTCCG GAAATGCCTG AAGGATGAAG ACCCATATGT GCGCAAGACA 
GCAGCTGTGT GTGTAGCCAA GCTCCACGAC ATCAACGCCC AGCTGGTAGA GGACCAGGGT 
TTCCTGGACA CACTCAAAGA CCTCATCTCT GACTCTAACC CCATGGTGGT AGCAAATGCA 
GTGGCTGCCC TGTCAGAGAT CGCTGAGTCT CACCCCAGTA GCAACCTGCT TGACTTGAAC 
CCACAGTCTA TCAACAAGCT GCTGACAGCC CTTAATGAGT GCACCGAGTG GGGCCAGATC 
TTCATTCTGG ACTGTCTGGC GAACTACATG CCCAAGGATG ACCGTGAGGC CCAGAGCATC 
TGCGAGCGGG TCACTCCTAG GCTTTCCCAT GCCAACTCTG CTGTGGTGCT GTCCGCAGTG 
AAGGTCCTGA TGAAGTTCAT GGAGATGCTG TCTAAGGACC TGGACTACTA TGCCACACTA 
CTCAAGAAGC TGGCCCCTCC CCTGGTCACA CTGCTGTCAG CAGAGCCAGA GTTGCAGTAT 
GTGGCTCTGC GCAATATTAA CCTCATTGTA CAGAAAAGGC CAGAGATTTT GAAGCATGAA 
ATGAAGGTTT TCTTTGTGAA GTACAATGAC CCCATCTATG TGAAGCTGGA GAAACTGGAC 
ATTATGATCC GCCTGGCCTC CCAGGCCAAC ATTGCCCAGG TTTTGGCAGA ACTCAAAGAG 
TACGCGACAG AAGTAGATGT GGATTTTGTA CGGAAGGCAG TGCGTGCCAT TGGCCGCTGT 
GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CATTGCTTGA TCTCATCCAG 
ACCAAGGTCA ACTATGTGGT CCAGGAAGCC ATTGTGGTCA TTAAAGACAT CTTCCGCAAG 
TACCCCAACA AGTACGAGAG TGTGATAGCC ACACTGTGTG AGAATCTGGA CTCGCTGGAT 
GAGCCTGAGG CTCGCGCTGC TATGATCTGG ATAGTGGGCG AGTATGCTGA GCGGATTGAC 
AATGCGGACG AGCTGCTTGA GAGCTTCCTC GACGGCTTCC ATGACGAGAG CACCCAGGTC 
CAGCTACAGC TGCTGACAGC CATTGTGAAG CTCTTCCTGA AGAAGCCAAC AGAGACGCAG 
GAGCTGGTGC AGCAGGTCCT CAGTTTGGCC ACTCAGGACT CGGACAATCC AGACCTTCGG 
GACCGTGGCT ACATCTACTG GCGCCTGCTC TCCACGGACC CTGTAGCTGC CAAAGAGGTG 
GTACTGGCTG AGAAGCCCCT CATCTCAGAA GAGACAGACC TTATTGAGCC CACACTGCTG 
GATGAACTCA TCTGCTACAT TGGCACACTG GCCTCTGTCT ACCACAAGCC ACCCAATGCC 
TTTGTGGAGG GCGGCCGGGG AGTGGTGCAC AAGAGTCTGC CACCTCGCAC TGCCTCGAGC 
GAAAGCACAG AGAGCCCTGA GGCAGCCCCT GCCGGAGCAC CTGCTAGTGA CCAGCCTGAT 
GTCATTCCTG CCCAGGGTGA CCTGTTAGGT GACCTCCTCA ACCTGGACCT TGGGCCCCCA 
GTGAGTGGCC CACCCTTGGC TGCCTCCTCA GTGCAGATGG GAGCTGTGGA TCTTCTTGGT 
GGTGGCCTCG ACAGCCTGAT GGGGGATGAG CCTGAAGGGA TTGGAGACAG CAACTTTGGG 
GCACCTCCAG CATCAGTGGC GGCGGCAGCA CCAGCCAGAC TTGGAGCACC CGTCAGCAGT 
GGCTTAAGTG ACCTCTTTGA CCTGACCAGT GGAGTGGGCA CCCTCTCAGG GTCCTATGTC 
GCTCCCAAAG CAGTTTGGCT CCCTGCCATG AAGGCCAAGG GGCTGGAAAT CTCTGGCACC 
TTCACCCGGC AGGTTGGCTC CATCTCCATG GATCTGCAGC TGACTAACAA GGCCCTGCAG 
GTCATGACAG ATTTCGCTAT CCAGTTTAAT CGGAACAGCT TTGGCTTGGC CCCCGCTGCG 
CCACTCCAAG TGCACGCCCC ACTGAGCCCC AACCAGACGG TGGAGATCTC CCTGCCTCTT 
AACACGGTGG GCTCAGTCAT GAAGATGGAG CCTCTGAACA ATCTTCAGGT GGCTGTGAAG 
AACAACATTG ACGTCTTCTA CTTCAGCACT TTGTACCCAC TGCATGTCCT CTTCGTGGAG 
GACGGGAAGA TGGACCGGCA GATGTTCCTG GCCACATGGA AGGACATTCC CAACGAGAAT 
GAAGCCCAGT TCCAGATCAG AGACTGCCCG CTGAACACAG AGGCTGCAAG CAGCAAGCTG 
CAAAGCAGTA ACATTTTCAC TGTCGCCAAG AGGACTGTGG AGGGCCAGGA CATGCTCTAC 
CAATCTCTGA AGTTGACCAA TGGCATCTGG GTGCTGGCAG AGCTGCGGAT CCAGCCAGGC 
AACCCCAGTT TCACGCTGTC CCTGAAGTGT CGAGCGCCAG AGGTGTCCCA GCATGTATAC 
CAGGCCTACG AGACCATCCT CAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295227.1
CDS	228..3077
Translation	MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYATLLKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLDGFHDESTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPNAFVEGGRGVVHKSLPPRTASSESTESPEAAPAGAPASDQPDVIPAQGDLLGDLLNLDLGPPVSGPPLAASSVQMGAVDLLGGGLDSLMGDEPEGIGDSNFGAPPASVAAAAPARLGAPVSSGLSDLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLNTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHVLFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNTEAASSKLQSSNIFTVAKRTVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSFTLSLKCRAPEVSQHVYQAYETILKN

Target ORF information:

RefSeq Version	NM_001308298.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor related protein complex 1 subunit beta 1 (Ap1b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308298.1

ORF Insert Sequence:

ATGACTGACT CAAAATACTT CACAACAACC AAGAAAGGGG AGATCTTCGA GTTGAAGGCA 
GAGCTTAACA GTGACAAGAA GGAGAAGAAG AAGGAGGCAG TAAAGAAAGT GATTGCATCA 
ATGACCGTGG GCAAAGATGT CAGTGCTCTC TTCCCTGATG TGGTGAACTG CATGCAGACA 
GACAACCTGG AGCTAAAGAA GCTGGTGTAC CTGTACTTGA TGAACTATGC CAAGAGTCAG 
CCTGACATGG CCATTATGGC TGTCAACACC TTCGTGAAGG ACTGTGAGGA CCCCAACCCC 
CTTATCCGGG CCCTGGCTGT GAGGACTATG GGCTGCATCC GAGTTGACAA GATCACAGAG 
TACTTGTGTG AACCACTCCG GAAATGCCTG AAGGATGAAG ACCCATATGT GCGCAAGACA 
GCAGCTGTGT GTGTAGCCAA GCTCCACGAC ATCAACGCCC AGCTGGTAGA GGACCAGGGT 
TTCCTGGACA CACTCAAAGA CCTCATCTCT GACTCTAACC CCATGGTGGT AGCAAATGCA 
GTGGCTGCCC TGTCAGAGAT CGCTGAGTCT CACCCCAGTA GCAACCTGCT TGACTTGAAC 
CCACAGTCTA TCAACAAGCT GCTGACAGCC CTTAATGAGT GCACCGAGTG GGGCCAGATC 
TTCATTCTGG ACTGTCTGGC GAACTACATG CCCAAGGATG ACCGTGAGGC CCAGAGCATC 
TGCGAGCGGG TCACTCCTAG GCTTTCCCAT GCCAACTCTG CTGTGGTGCT GTCCGCAGTG 
AAGGTCCTGA TGAAGTTCAT GGAGATGCTG TCTAAGGACC TGGACTACTA TGCCACACTA 
CTCAAGAAGC TGGCCCCTCC CCTGGTCACA CTGCTGTCAG CAGAGCCAGA GTTGCAGTAT 
GTGGCTCTGC GCAATATTAA CCTCATTGTA CAGAAAAGGC CAGAGATTTT GAAGCATGAA 
ATGAAGGTTT TCTTTGTGAA GTACAATGAC CCCATCTATG TGAAGCTGGA GAAACTGGAC 
ATTATGATCC GCCTGGCCTC CCAGGCCAAC ATTGCCCAGG TTTTGGCAGA ACTCAAAGAG 
TACGCGACAG AAGTAGATGT GGATTTTGTA CGGAAGGCAG TGCGTGCCAT TGGCCGCTGT 
GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CATTGCTTGA TCTCATCCAG 
ACCAAGGTCA ACTATGTGGT CCAGGAAGCC ATTGTGGTCA TTAAAGACAT CTTCCGCAAG 
TACCCCAACA AGTACGAGAG TGTGATAGCC ACACTGTGTG AGAATCTGGA CTCGCTGGAT 
GAGCCTGAGG CTCGCGCTGC TATGATCTGG ATAGTGGGCG AGTATGCTGA GCGGATTGAC 
AATGCGGACG AGCTGCTTGA GAGCTTCCTC GACGGCTTCC ATGACGAGAG CACCCAGGTC 
CAGCTACAGC TGCTGACAGC CATTGTGAAG CTCTTCCTGA AGAAGCCAAC AGAGACGCAG 
GAGCTGGTGC AGCAGGTCCT CAGTTTGGCC ACTCAGGACT CGGACAATCC AGACCTTCGG 
GACCGTGGCT ACATCTACTG GCGCCTGCTC TCCACGGACC CTGTAGCTGC CAAAGAGGTG 
GTACTGGCTG AGAAGCCCCT CATCTCAGAA GAGACAGACC TTATTGAGCC CACACTGCTG 
GATGAACTCA TCTGCTACAT TGGCACACTG GCCTCTGTCT ACCACAAGCC ACCCAATGCC 
TTTGTGGAGG GCGGCCGGGG AGTGGTGCAC AAGAGTCTGC CACCTCGCAC TGCCTCGAGC 
GAAAGCACAG AGAGCCCTGA GGCAGCCCCT GCCGGAGCAC CTGCTAGTGA CCAGCCTGAT 
GTCATTCCTG CCCAGGGTGA CCTGTTAGGT GACCTCCTCA ACCTGGACCT TGGGCCCCCA 
GTGAGTGGCC CACCCTTGGC TGCCTCCTCA GTGCAGATGG GAGCTGTGGA TCTTCTTGGT 
GGTGGCCTCG ACAGCCTGAT GGGGGATGAG CCTGAAGGGA TTGGAGACAG CAACTTTGGG 
GCACCTCCAG CATCAGTGGC GGCGGCAGCA CCAGCCAGAC TTGGAGCACC CGTCAGCAGT 
GGCTTAAGTG ACCTCTTTGA CCTGACCAGT GGAGTGGGCA CCCTCTCAGG GTCCTATGTC 
GCTCCCAAAG CAGTTTGGCT CCCTGCCATG AAGGCCAAGG GGCTGGAAAT CTCTGGCACC 
TTCACCCGGC AGGTTGGCTC CATCTCCATG GATCTGCAGC TGACTAACAA GGCCCTGCAG 
GTCATGACAG ATTTCGCTAT CCAGTTTAAT CGGAACAGCT TTGGCTTGGC CCCCGCTGCG 
CCACTCCAAG TGCACGCCCC ACTGAGCCCC AACCAGACGG TGGAGATCTC CCTGCCTCTT 
AACACGGTGG GCTCAGTCAT GAAGATGGAG CCTCTGAACA ATCTTCAGGT GGCTGTGAAG 
AACAACATTG ACGTCTTCTA CTTCAGCACT TTGTACCCAC TGCATGTCCT CTTCGTGGAG 
GACGGGAAGA TGGACCGGCA GATGTTCCTG GCCACATGGA AGGACATTCC CAACGAGAAT 
GAAGCCCAGT TCCAGATCAG AGACTGCCCG CTGAACACAG AGGCTGCAAG CAGCAAGCTG 
CAAAGCAGTA ACATTTTCAC TGTCGCCAAG AGGACTGTGG AGGGCCAGGA CATGCTCTAC 
CAATCTCTGA AGTTGACCAA TGGCATCTGG GTGCTGGCAG AGCTGCGGAT CCAGCCAGGC 
AACCCCAGTT TCACGCTGTC CCTGAAGTGT CGAGCGCCAG AGGTGTCCCA GCATGTATAC 
CAGGCCTACG AGACCATCCT CAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
The Journal of neuroscience : the official journal of the Society for Neuroscience31(6)2225-37(2011 Feb)
Piccoli G,Condliffe SB,Bauer M,Giesert F,Boldt K,De Astis S,Meixner A,Sarioglu H,Vogt-Weisenhorn DM,Wurst W,Gloeckner CJ,Matteoli M,Sala C,Ueffing M

Alternate pathways preserve tumor necrosis factor-alpha production after nuclear factor-kappaB inhibition in neonatal cerebral hypoxia-ischemia.
Stroke40(10)3362-8(2009 Oct)
Nijboer CH,Heijnen CJ,Groenendaal F,van Bel F,Kavelaars A

Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]58(3)143-50(2009 Mar)
Jung KJ,Lee EK,Kim JY,Zou Y,Sung B,Heo HS,Kim MK,Lee J,Kim ND,Yu BP,Chung HY

Matrix metalloproteinase-3 induction in rat brain astrocytes: focus on the role of two AP-1 elements.
The Biochemical journal410(3)605-11(2008 Mar)
Kim KS,Kim HY,Joe EH,Jou I

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Ap1b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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