Cask cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cask
Entrez Gene ID	29647
Full Name	calcium/calmodulin dependent serine protein kinase
General protein information	Preferred Names calcium/calmodulin dependent serine protein kinase Names peripheral plasma membrane protein CASK calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12
Summary	may play a role in intracellular Ca(2+) mediated signaling pathways [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022184.1	NP_071520.1	peripheral plasma membrane protein CASK

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001683	XP_001238476.3
Canis lupus familiaris (dog)	CASK	XP_005641318.1
Xenopus tropicalis (tropical clawed frog)	cask	NP_001016204.1
Homo sapiens (human)	CASK	NP_003679.2
Pan troglodytes (chimpanzee)	CASK	XP_003317477.2
	CASK	XP_001095837.2
Rattus norvegicus (Norway rat)	Cask	NP_071520.1
Danio rerio (zebrafish)	caska	NP_694420.1
Drosophila melanogaster (fruit fly)	CASK	NP_001262811.1
Bos taurus (cattle)	CASK	NP_001095951.1
Mus musculus (house mouse)	Cask	NP_001271432.1
Gallus gallus (chicken)	CASK	XP_416769.3
Caenorhabditis elegans (roundworm)	lin-2	NP_001024587.1

Gene Ontology
Bibliography

Related articles in PubMed

Forkhead box O1 mediates defects in palmitate-induced insulin granule exocytosis by downregulation of calcium/calmodulin-dependent serine protein kinase expression in INS-1 cells.
Wang Y, Lin H, Hao N, Zhu Z, Wang D, Li Y, Chen H, Zhu Y, Han X
Diabetologia58(6)1272-81(2015 Jun)

Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4-induced insulin secretion in INS-1 cells.
Zhu ZQ, Wang D, Xiang D, Yuan YX, Wang Y
Metabolism: clinical and experimental63(1)120-6(2014 Jan)

Traumatic brain injury dysregulates microRNAs to modulate cell signaling in rat hippocampus.
Liu L, Sun T, Liu Z, Chen X, Zhao L, Qu G, Li Q
PloS one9(8)e103948(2014)

Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.
Landgraf P, Mikhaylova M, Macharadze T, Borutzki C, Zenclussen AC, Wahle P, Kreutz MR
PloS one9(2)e85924(2014)

Liprin-?2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology201(6)915-28(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

induction of beta cell insulin-secretion defects by fatty acids is mediated, at least in part, by FOXO1 via downregulation of Cask expression
Title: Forkhead box O1 mediates defects in palmitate-induced insulin granule exocytosis by downregulation of calcium/calmodulin-dependent serine protein kinase expression in INS-1 cells.

Our study suggests that the stimulation of beta-cell insulin secretion by Ex-4 is mediated, at least in part, by CASK via a novel signaling mechanism.
Title: Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4-induced insulin secretion in INS-1 cells.

Data suggest that Y-P30 can control the nuclear localization of Calcium/calmodulin-dependent serine kinase (CASK) in a syndecan (SDC) -2 and -3 -dependent manner.
Title: Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.

The findings suggest that CASK and the truncated prestin splice isoform contribute to confinement of prestin to the basolateral region of the plasma membrane.
Title: Generation of somatic electromechanical force by outer hair cells may be influenced by prestin-CASK interaction at the basal junction with the Deiter's cell.

Depletion of liprin-alpha2 resulted in reduced turnover of RIM1 and CASK at presynaptic terminals, suggesting that liprin-alpha2 promotes dynamic scaffolding for molecular complexes that facilitate synaptic vesicle release.
Title: Liprin-?2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.

The following Cask gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cask cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05378	NM_022184.1 Latest version!	Rattus norvegicus calcium/calmodulin dependent serine protein kinase (Cask), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cask ( NM_022184.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071520.1 Rattus norvegicus calcium/calmodulin dependent serine protein kinase (Cask), mRNA.

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CloneID	ORa05378
Clone ID Related Accession (Same CDS sequence)	NM_022184.1
Accession Version	NM_022184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	peripheral plasma membrane protein CASK
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U47110.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U47110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGCAAG 
GGTCCCTTCA GTGTTGTACG ACGATGCATC AACAGAGAAA CTGGACAACA ATTTGCTGTA 
AAAATTGTTG ACGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG 
CGGGAAGCCA GTATCTGTCA TATGCTGAAG CATCCACACA TTGTAGAGCT GTTGGAGACA 
TATAGCTCAG ATGGGATGCT TTACATGGTT TTTGAATTTA TGGATGGAGC AGATCTGTGT 
TTTGAAATTG TAAAGCGAGC TGATGCTGGT TTTGTATACA GTGAAGCTGT AGCCAGTCAC 
TACATGAGAC AGATACTGGA AGCTCTACGC TACTGTCATG ATAATAACAT AATTCACAGG 
GATGTGAAGC CCCACTGTGT TCTCCTCGCC TCAAAAGAAA ATTCTGCACC TGTTAAGCTT 
GGGGGCTTTG GGGTAGCCAT CCAGTTAGGA GAATCTGGAC TTGTTGCTGG AGGACGCGTT 
GGAACACCTC ACTTTATGGC CCCAGAAGTG GTCAAGAGAG AGCCTTATGG AAAGCCTGTG 
GATGTCTGGG GCTGCGGTGT GATTCTTTTC ATCCTGCTCA GCGGCTGTTT GCCTTTCTAC 
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG 
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTGC GCCGCATGCT GATGCTGGAT 
CCTGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT 
CGTTATGCCT ACAAAATTCA TCTTCCAGAA ACAGTAGAAC AACTGAGGAA ATTCAATGCA 
AGGAGAAAAC TAAAGGGTGC AGTACTAGCG GCTGTGTCAA GTCACAAATT CAATTCCTTC 
TACGGGGACC CTCCTGAAGA GTTGCCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT 
CTAGCAGCAG AAAGAGCCGT CTCTCAGGTG CTGGACAGCC TGGAAGAGAT TCACGCACTT 
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT 
CACACACTGC TGGATCTGTA TGACAAAATT AACACAAAGT CTTCGCCACA AATCAGGAAT 
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT 
GAGAATAATG ACGCGAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA 
CTTCAGACTC ACCACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT AAGGGTCACA 
CCTCCGCCGA CTTCCCCGTA TTTAAACGGT GATTCTCCAG AAAGTGCAAA TGGAGACATG 
GACATGGAGA ATGTGACCAG AGTTCGCCTG GTACAGTTTC AAAAGAACAC AGATGAGCCA 
ATGGGAATCA CTTTGAAAAT GAATGAGCTC AATCATTGTA TTGTTGCGAG AATCATGCAT 
GGCGGGATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATCCG AGAAATCAAT 
GGCATCAGTG TCGCTAACCA AACAGTGGAA CAGCTACAAA AAATGCTTAG GGAAATGCGA 
GGGAGTATTA CCTTCAAGAT TGTGCCAAGC TACCGCACTC AGTCTTCGTC ATGTGAGAGA 
GATTCCCCCT CCACTTCCAG ACAGTCCCCT GCTAATGGTC ATAGCAGCAC TAACAATTCT 
GTTTCGATCT ATGTAAGAGC ACAATTTGAA TATGATCCAG CCAAGGATGA CCTCATCCCC 
TGCAAAGAAG CTGGCATCCG GTTCCGAGTT GGTGACATCA TCCAGATTAT TAGTAAGGAT 
GACCACAACT GGTGGCAGGG TAAACTGGAA AACTCCAAAA ACGGAACTGC AGGTCTCATT 
CCTTCTCCTG AACTTCAGGA ATGGCGAGTA GCTTGCATTG CCATGGAGAA GACCAAACAA 
GAGCAGCAGG CCAGCTGTAC TTGGTTTGGC AAGAAAAAGA AGCAGTACAA AGATAAATAT 
TTGGCAAAGC ACAATGCAGA TCTTGTCACG TATGAAGAAG TAGTGAAACT GCCAGCGTTC 
AAAAGGAAAA CATTAGTCTT ATTAGGTGCA CATGGTGTTG GGAGAAGACA CATCAAAAAC 
ACCCTCATCA CAAAGCACCC GGAGCGCTTC GCGTACCCCA TTCCGCATAC AACCAGACCT 
CCAAAGAAAG ATGAAGAAAA TGGCAAGAAT TATTACTTTG TATCTCATGA CCAAATGATG 
CAAGATATCT CAAATAATGA ATACTTGGAG TACGGCAGCC ATGAGGATGC AATGTATGGG 
ACAAAACTGG AGACCATTCG GAAAATCCAT GAGCAGGGGC TGATTGCGAT TCTGGACGTG 
GAGCCTCAGG CATTGAAGGT CCTGAGAACT GCAGAGTTTC CCCCTTTTGT TGTCTTCATT 
GCGGCGCCAA CTATCACTCC AGGTTTAAAT GAGGATGAAT CTCTTCAGCG CCTGCAGAAG 
GAGTCCGATG TCTTGCAGAG AACATATGCA CACTACTTCG ATCTCACAAT TATCAACAAT 
GAAATTGATG AGACAATCAG ACATCTGGAG GAAGCTGTCG AGCTTGTGTG CACAGCCCCA 
CAGTGGGTCC CGGTCTCCTG GGTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071520.1
CDS	358..3087
Translation	MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQVLDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHHVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFKIVPSYRTQSSSCERDSPSTSRQSPANGHSSTNNSVSIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNADLVTYEEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPERFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFPPFVVFIAAPTITPGLNEDESLQRLQKESDVLQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY

Target ORF information:

RefSeq Version	NM_022184.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium/calmodulin dependent serine protein kinase (Cask), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022184.1

ORF Insert Sequence:

ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGCAAG 
GGTCCCTTCA GTGTTGTACG ACGATGCATC AACAGAGAAA CTGGACAACA ATTTGCTGTA 
AAAATTGTTG ACGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG 
CGGGAAGCCA GTATCTGTCA TATGCTGAAG CATCCACACA TTGTAGAGCT GTTGGAGACA 
TATAGCTCAG ATGGGATGCT TTACATGGTT TTTGAATTTA TGGATGGAGC AGATCTGTGT 
TTTGAAATTG TAAAGCGAGC TGATGCTGGT TTTGTATACA GTGAAGCTGT AGCCAGTCAC 
TACATGAGAC AGATACTGGA AGCTCTACGC TACTGTCATG ATAATAACAT AATTCACAGG 
GATGTGAAGC CCCACTGTGT TCTCCTCGCC TCAAAAGAAA ATTCTGCACC TGTTAAGCTT 
GGGGGCTTTG GGGTAGCCAT CCAGTTAGGA GAATCTGGAC TTGTTGCTGG AGGACGCGTT 
GGAACACCTC ACTTTATGGC CCCAGAAGTG GTCAAGAGAG AGCCTTATGG AAAGCCTGTG 
GATGTCTGGG GCTGCGGTGT GATTCTTTTC ATCCTGCTCA GCGGCTGTTT GCCTTTCTAC 
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG 
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTGC GCCGCATGCT GATGCTGGAT 
CCTGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT 
CGTTATGCCT ACAAAATTCA TCTTCCAGAA ACAGTAGAAC AACTGAGGAA ATTCAATGCA 
AGGAGAAAAC TAAAGGGTGC AGTACTAGCG GCTGTGTCAA GTCACAAATT CAATTCCTTC 
TACGGGGACC CTCCTGAAGA GTTGCCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT 
CTAGCAGCAG AAAGAGCCGT CTCTCAGGTG CTGGACAGCC TGGAAGAGAT TCACGCACTT 
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT 
CACACACTGC TGGATCTGTA TGACAAAATT AACACAAAGT CTTCGCCACA AATCAGGAAT 
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT 
GAGAATAATG ACGCGAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA 
CTTCAGACTC ACCACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT AAGGGTCACA 
CCTCCGCCGA CTTCCCCGTA TTTAAACGGT GATTCTCCAG AAAGTGCAAA TGGAGACATG 
GACATGGAGA ATGTGACCAG AGTTCGCCTG GTACAGTTTC AAAAGAACAC AGATGAGCCA 
ATGGGAATCA CTTTGAAAAT GAATGAGCTC AATCATTGTA TTGTTGCGAG AATCATGCAT 
GGCGGGATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATCCG AGAAATCAAT 
GGCATCAGTG TCGCTAACCA AACAGTGGAA CAGCTACAAA AAATGCTTAG GGAAATGCGA 
GGGAGTATTA CCTTCAAGAT TGTGCCAAGC TACCGCACTC AGTCTTCGTC ATGTGAGAGA 
GATTCCCCCT CCACTTCCAG ACAGTCCCCT GCTAATGGTC ATAGCAGCAC TAACAATTCT 
GTTTCGATCT ATGTAAGAGC ACAATTTGAA TATGATCCAG CCAAGGATGA CCTCATCCCC 
TGCAAAGAAG CTGGCATCCG GTTCCGAGTT GGTGACATCA TCCAGATTAT TAGTAAGGAT 
GACCACAACT GGTGGCAGGG TAAACTGGAA AACTCCAAAA ACGGAACTGC AGGTCTCATT 
CCTTCTCCTG AACTTCAGGA ATGGCGAGTA GCTTGCATTG CCATGGAGAA GACCAAACAA 
GAGCAGCAGG CCAGCTGTAC TTGGTTTGGC AAGAAAAAGA AGCAGTACAA AGATAAATAT 
TTGGCAAAGC ACAATGCAGA TCTTGTCACG TATGAAGAAG TAGTGAAACT GCCAGCGTTC 
AAAAGGAAAA CATTAGTCTT ATTAGGTGCA CATGGTGTTG GGAGAAGACA CATCAAAAAC 
ACCCTCATCA CAAAGCACCC GGAGCGCTTC GCGTACCCCA TTCCGCATAC AACCAGACCT 
CCAAAGAAAG ATGAAGAAAA TGGCAAGAAT TATTACTTTG TATCTCATGA CCAAATGATG 
CAAGATATCT CAAATAATGA ATACTTGGAG TACGGCAGCC ATGAGGATGC AATGTATGGG 
ACAAAACTGG AGACCATTCG GAAAATCCAT GAGCAGGGGC TGATTGCGAT TCTGGACGTG 
GAGCCTCAGG CATTGAAGGT CCTGAGAACT GCAGAGTTTC CCCCTTTTGT TGTCTTCATT 
GCGGCGCCAA CTATCACTCC AGGTTTAAAT GAGGATGAAT CTCTTCAGCG CCTGCAGAAG 
GAGTCCGATG TCTTGCAGAG AACATATGCA CACTACTTCG ATCTCACAAT TATCAACAAT 
GAAATTGATG AGACAATCAG ACATCTGGAG GAAGCTGTCG AGCTTGTGTG CACAGCCCCA 
CAGTGGGTCC CGGTCTCCTG GGTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Forkhead box O1 mediates defects in palmitate-induced insulin granule exocytosis by downregulation of calcium/calmodulin-dependent serine protein kinase expression in INS-1 cells.
Diabetologia58(6)1272-81(2015 Jun)
Wang Y,Lin H,Hao N,Zhu Z,Wang D,Li Y,Chen H,Zhu Y,Han X

Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4-induced insulin secretion in INS-1 cells.
Metabolism: clinical and experimental63(1)120-6(2014 Jan)
Zhu ZQ,Wang D,Xiang D,Yuan YX,Wang Y

Traumatic brain injury dysregulates microRNAs to modulate cell signaling in rat hippocampus.
PloS one9(8)e103948(2014)
Liu L,Sun T,Liu Z,Chen X,Zhao L,Qu G,Li Q

Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.
PloS one9(2)e85924(2014)
Landgraf P,Mikhaylova M,Macharadze T,Borutzki C,Zenclussen AC,Wahle P,Kreutz MR

Liprin-?2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
The Journal of cell biology201(6)915-28(2013 Jun)
Spangler SA,Schmitz SK,Kevenaar JT,de Graaff E,de Wit H,Demmers J,Toonen RF,Hoogenraad CC

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Cask cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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