Fxyd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fxyd2
Entrez Gene ID	29639
Full Name	FXYD domain-containing ion transport regulator 2
Synonyms	ATP1C,Atp1g1,GNAKATP
General protein information	Preferred Names FXYD domain-containing ion transport regulator 2 Names sodium/potassium-transporting ATPase subunit gamma ATPase, Na+/K+ transporting, gamma 1 polypeptide FXTD domain-containing ion transport regulator 2 Na(+)/K(+) ATPase subunit gamma gamma subunit of sodium potassium ATPase sodium pump gamma chain sodium/potassium-transporting ATPase gamma chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q22
Summary	This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. This gene, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. Related gene family members have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members, with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The Type III integral membrane protein encoded by this gene is the gamma subunit of the Na,K-ATPase present on the plasma membrane. Although the Na,K-ATPase does not depend on the gamma subunit to be functional, it is thought that the gamma subunit modulates the enzyme's activity by inducing ion channel activity. Two transcript variants have been described for this gene that encode distinct isoforms. [provided by RefSeq, Jan 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_145717.1	NP_663769.1	sodium/potassium-transporting ATPase subunit gamma isoform a
XM_006242911.3	XP_006242973.1	sodium/potassium-transporting ATPase subunit gamma isoform X1
NM_017349.2	NP_059045.2	sodium/potassium-transporting ATPase subunit gamma isoform b
XM_006242912.3	XP_006242974.1	sodium/potassium-transporting ATPase subunit gamma isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FXYD2	NP_067614.1
Pan troglodytes (chimpanzee)	FXYD2	XP_001158882.2
	FXYD2	XP_001092388.2
Rattus norvegicus (Norway rat)	Fxyd2	NP_059045.2
Canis lupus familiaris (dog)	FXYD2	NP_001239266.1
Bos taurus (cattle)	FXYD2	NP_776745.2

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional control of Na,K-ATPase activity and cell growth by a splice variant of FXYD2 protein with modified mRNA.
Sweadner KJ, Pascoa JL, Salazar CA, Arystarkhova E
The Journal of biological chemistry286(20)18290-300(2011 May)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Locations, abundances, and possible functions of FXYD ion transport regulators in rat renal medulla.
Pihakaski-Maunsbach K, Vorum H, Honor? B, Tokonabe S, Fr?kiaer J, Garty H, Karlish SJ, Maunsbach AB
American journal of physiology. Renal physiology291(5)F1033-44(2006 Nov)

Structural interactions between FXYD proteins and Na+,K+-ATPase: alpha/beta/FXYD subunit stoichiometry and cross-linking.
Lindzen M, Gottschalk KE, F?zesi M, Garty H, Karlish SJ
The Journal of biological chemistry281(9)5947-55(2006 Mar)

Na,K-ATPase from mice lacking the gamma subunit (FXYD2) exhibits altered Na+ affinity and decreased thermal stability.
Jones DH, Li TY, Arystarkhova E, Barr KJ, Wetzel RK, Peng J, Markham K, Sweadner KJ, Fong GH, Kidder GM
The Journal of biological chemistry280(19)19003-11(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Post-transcriptional control of Na,K-ATPase activity and cell growth by a splice variant of FXYD2 protein with modified mRNA.
Title: Post-transcriptional control of Na,K-ATPase activity and cell growth by a splice variant of FXYD2 protein with modified mRNA.

the unchanged levels of FXYD2 and FXYD4 proteins suggest that they are not primary determinants for urine electrolyte composition during NaCl depletion.
Title: Locations, abundances, and possible functions of FXYD ion transport regulators in rat renal medulla.

Data describe the interactions of rat FXYD1,-2, and -4 proteins with rat alpha1 subunits of Na(+),K(+)-ATPase.
Title: Structural interactions between FXYD proteins and Na+,K+-ATPase: alpha/beta/FXYD subunit stoichiometry and cross-linking.

stress inducers (heat shock, exogenous oxidation, and chemical stress) resulted in a selective induction of gammaa isoform and a significant reduction (up to 60%) of Na,K-ATPase activity
Title: Stress-induced expression of the gamma subunit (FXYD2) modulates Na,K-ATPase activity and cell growth.

the TM domain of FYXD2 effects the shift in apparent Na+ affinity
Title: Modulation of Na,K-ATPase by the gamma subunit: studies with transfected cells and transmembrane mimetic peptides.

The following Fxyd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fxyd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02630	NM_145717.1 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa03731	NM_017349.2 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa02630	XM_006242911.3 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa31064	XM_006242912.3 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fxyd2 ( NM_145717.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_663769.1 Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.

Price & Availability↑

CloneID	ORa02630
Clone ID Related Accession (Same CDS sequence)	NM_145717.1 , XM_006242911.3
Accession Version	NM_145717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit gamma isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF129400.1. Transcript Variant: This variant (a) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF129400.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGACAGAGC TGTCAGCTAA CCATGGTGGC AGTGCCAAGG GGACGGAGAA TCCCTTCGAG 
TATGACTATG AAACCGTCCG CAAAGGAGGC CTGATCTTCG CGGGCCTTGC CTTCGTCGTG 
GGACTCCTCA TTCTCCTCAG CAAAAGATTC CGCTGTGGGG GCAGTAAGAA GCATAGGCAG 
GTCAATGAAG ATGAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663769.1
CDS	39..239
Translation	MTELSANHGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLILLSKRFRCGGSKKHRQVNEDEL

Target ORF information:

RefSeq Version	NM_145717.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145717.1

ORF Insert Sequence:

ATGACAGAGC TGTCAGCTAA CCATGGTGGC AGTGCCAAGG GGACGGAGAA TCCCTTCGAG 
TATGACTATG AAACCGTCCG CAAAGGAGGC CTGATCTTCG CGGGCCTTGC CTTCGTCGTG 
GGACTCCTCA TTCTCCTCAG CAAAAGATTC CGCTGTGGGG GCAGTAAGAA GCATAGGCAG 
GTCAATGAAG ATGAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd2 ( NM_017349.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059045.2 Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.

Price & Availability↑

CloneID	ORa03731
Clone ID Related Accession (Same CDS sequence)	NM_017349.2
Accession Version	NM_017349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit gamma isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF233060.1. On Aug 29, 2002 this sequence version replaced NM_017349.1. Transcript Variant: This variant (b) contains an alternate exon in the 5' coding region and 5' UTR, compared to variant a. This results in a shorter protein (isoform b) that has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF233060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689596 [ECO:0000348] ##Evidence-Data-END##

ATGGACAGGT GGTACTTGGG TGGCAGTGCC AAGGGGACGG AGAATCCCTT CGAGTATGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TTGCCTTCGT CGTGGGACTC 
CTCATTCTCC TCAGCAAAAG ATTCCGCTGT GGGGGCAGTA AGAAGCATAG GCAGGTCAAT 
GAAGATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059045.2
CDS	1..195
Translation	MDRWYLGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLILLSKRFRCGGSKKHRQVNEDEL

Target ORF information:

RefSeq Version	NM_017349.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017349.2

ORF Insert Sequence:

ATGGACAGGT GGTACTTGGG TGGCAGTGCC AAGGGGACGG AGAATCCCTT CGAGTATGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TTGCCTTCGT CGTGGGACTC 
CTCATTCTCC TCAGCAAAAG ATTCCGCTGT GGGGGCAGTA AGAAGCATAG GCAGGTCAAT 
GAAGATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd2 ( XM_006242911.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242973.1 Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02630
Clone ID Related Accession (Same CDS sequence)	NM_145717.1 , XM_006242911.3
Accession Version	XM_006242911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGC TGTCAGCTAA CCATGGTGGC AGTGCCAAGG GGACGGAGAA TCCCTTCGAG 
TATGACTATG AAACCGTCCG CAAAGGAGGC CTGATCTTCG CGGGCCTTGC CTTCGTCGTG 
GGACTCCTCA TTCTCCTCAG CAAAAGATTC CGCTGTGGGG GCAGTAAGAA GCATAGGCAG 
GTCAATGAAG ATGAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242973.1
CDS	194..394
Translation	MTELSANHGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLILLSKRFRCGGSKKHRQVNEDEL

Target ORF information:

RefSeq Version	XM_006242911.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242911.3

ORF Insert Sequence:

ATGACAGAGC TGTCAGCTAA CCATGGTGGC AGTGCCAAGG GGACGGAGAA TCCCTTCGAG 
TATGACTATG AAACCGTCCG CAAAGGAGGC CTGATCTTCG CGGGCCTTGC CTTCGTCGTG 
GGACTCCTCA TTCTCCTCAG CAAAAGATTC CGCTGTGGGG GCAGTAAGAA GCATAGGCAG 
GTCAATGAAG ATGAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd2 ( XM_006242912.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242974.1 Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31064
Clone ID Related Accession (Same CDS sequence)	XM_006242912.3
Accession Version	XM_006242912.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium/potassium-transporting ATPase subunit gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACGG AGAATCCCTT CGAGTATGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TTGCCTTCGT CGTGGGACTC 
CTCATTCTCC TCAGCAAAAG ATTCCGCTGT GGGGGCAGTA AGAAGCATAG GCAGGTCAAT 
GAAGATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242974.1
CDS	144..338
Translation	MDRWYLGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLILLSKRFRCGGSKKHRQVNEDEL

Target ORF information:

RefSeq Version	XM_006242912.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242912.3

ORF Insert Sequence:

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACGG AGAATCCCTT CGAGTATGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TTGCCTTCGT CGTGGGACTC 
CTCATTCTCC TCAGCAAAAG ATTCCGCTGT GGGGGCAGTA AGAAGCATAG GCAGGTCAAT 
GAAGATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional control of Na,K-ATPase activity and cell growth by a splice variant of FXYD2 protein with modified mRNA.
The Journal of biological chemistry286(20)18290-300(2011 May)
Sweadner KJ,Pascoa JL,Salazar CA,Arystarkhova E

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Locations, abundances, and possible functions of FXYD ion transport regulators in rat renal medulla.
American journal of physiology. Renal physiology291(5)F1033-44(2006 Nov)
Pihakaski-Maunsbach K,Vorum H,Honor? B,Tokonabe S,Fr?kiaer J,Garty H,Karlish SJ,Maunsbach AB

Structural interactions between FXYD proteins and Na+,K+-ATPase: alpha/beta/FXYD subunit stoichiometry and cross-linking.
The Journal of biological chemistry281(9)5947-55(2006 Mar)
Lindzen M,Gottschalk KE,F?zesi M,Garty H,Karlish SJ

Na,K-ATPase from mice lacking the gamma subunit (FXYD2) exhibits altered Na+ affinity and decreased thermal stability.
The Journal of biological chemistry280(19)19003-11(2005 May)
Jones DH,Li TY,Arystarkhova E,Barr KJ,Wetzel RK,Peng J,Markham K,Sweadner KJ,Fong GH,Kidder GM

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Fxyd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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