Gria2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gria2
Entrez Gene ID	29627
Full Name	glutamate ionotropic receptor AMPA type subunit 2
Synonyms	GluA2,GluR-K2,GluR2,gluR-B
General protein information	Preferred Names glutamate ionotropic receptor AMPA type subunit 2 Names glutamate receptor 2 AMPA-selective glutamate receptor 2 glutamate receptor B glutamate receptor, ionotropic, AMPA 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q33
Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083811.1	NP_001077280.1	glutamate receptor 2 isoform 2 precursor
NM_017261.2	NP_058957.1	glutamate receptor 2 isoform 1 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gria2	NP_001135539.1
	GRIA2	XP_001095129.2
Gallus gallus (chicken)	GRIA2	NP_001001775.2
Homo sapiens (human)	GRIA2	NP_000817.2
Pan troglodytes (chimpanzee)	GRIA2	NP_001171923.2
Mus musculus (house mouse)	Gria2	NP_001077275.1
Canis lupus familiaris (dog)	GRIA2	XP_539784.2
Bos taurus (cattle)	GRIA2	NP_001069789.2
Rattus norvegicus (Norway rat)	Gria2	NP_058957.1

Gene Ontology

The following Gria2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gria2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13198	NM_017261.2 Latest version!	Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
ORa13212	NM_001083811.1 Latest version!	Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gria2 ( NM_017261.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058957.1 Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13198
Clone ID Related Accession (Same CDS sequence)	NM_017261.2
Accession Version	NM_017261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor 2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M38061.1, AF164344.1, CO400088.1 and AABR07072744.1. On Apr 3, 2007 this sequence version replaced NM_017261.1. Transcript Variant: This variant (1) encodes isoform 1 (also known as the flip isoform). RNA editing changes Gln607Arg (CAG->CGG) and Arg764Gly (AGA->GGA). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M38061.1, AF164344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 7992055, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAGA TTATGCATAT TTCTGTCCTC CTTTCTCCTG TTTTATGGGG ACTGATTTTT 
GGTGTCTCTT CTAACAGCAT ACAGATAGGG GGGCTATTTC CAAGGGGCGC TGATCAAGAA 
TACAGTGCAT TTCGGGTAGG GATGGTTCAG TTTTCCACTT CGGAGTTCAG ACTGACACCC 
CATATCGACA ATTTGGAGGT AGCCAACAGT TTCGCAGTCA CCAATGCTTT CTGCTCCCAG 
TTTTCAAGAG GAGTCTACGC AATTTTTGGA TTTTATGACA AGAAGTCTGT AAATACCATC 
ACATCATTCT GTGGGACACT CCATGTGTCC TTCATCACAC CTAGCTTCCC AACAGATGGC 
ACACATCCAT TTGTCATCCA GATGCGACCT GACCTCAAAG GAGCACTCCT TAGCTTGATT 
GAGTACTACC AATGGGACAA GTTCGCATAC CTCTATGACA GTGACAGAGG CTTATCAACA 
CTGCAAGCTG TTCTGGATTC TGCTGCAGAG AAGAAGTGGC AGGTGACTGC TATCAATGTG 
GGGAACATCA ACAATGACAA GAAAGATGAG ACCTACAGAT CGCTCTTTCA AGATCTGGAG 
TTAAAAAAAG AACGGCGTGT AATCCTGGAC TGTGAAAGGG ATAAAGTAAA TGACATTGTG 
GACCAGGTTA TTACCATTGG AAAACATGTT AAAGGGTACC ATTATATCAT TGCAAATCTG 
GGATTCACTG ATGGGGACCT GCTGAAAATT CAGTTTGGAG GAGCAAATGT CTCTGGATTT 
CAGATTGTAG ACTACGACGA TTCCCTGGTG TCTAAATTTA TAGAAAGATG GTCAACACTG 
GAAGAGAAAG AATACCCTGG AGCACACACA GCGACAATTA AGTATACTTC GGCCCTGACG 
TATGATGCTG TCCAAGTGAT GACTGAAGCA TTCCGTAACC TTCGGAAGCA GAGGATTGAA 
ATATCCCGGA GAGGAAATGC AGGAGATTGT TTGGCCAACC CAGCTGTGCC CTGGGGACAA 
GGGGTCGAAA TAGAAAGGGC CCTCAAGCAG GTTCAAGTTG AAGGCCTCTC TGGAAATATA 
AAGTTTGACC AGAATGGAAA ACGAATAAAC TACACAATTA ACATCATGGA GCTCAAAACA 
AATGGACCCC GGAAGATTGG GTACTGGAGT GAAGTGGATA AAATGGTTGT CACCCTAACT 
GAGCTCCCAT CAGGAAATGA CACGTCTGGG CTTGAAAACA AGACTGTGGT GGTCACCACA 
ATATTGGAAT CTCCATATGT TATGATGAAG AAAAATCATG AAATGCTTGA AGGGAATGAG 
CGTTACGAGG GCTACTGTGT TGACTTAGCT GCAGAAATTG CTAAACACTG TGGGTTCAAG 
TACAAGCTGA CTATTGTTGG GGATGGCAAG TATGGGGCCA GGGATGCCGA CACCAAAATT 
TGGAATGGTA TGGTTGGAGA GCTTGTCTAC GGGAAAGCTG ACATTGCAAT TGCTCCATTA 
ACTATCACTC TCGTGAGAGA AGAGGTGATT GACTTCTCCA AGCCCTTCAT GAGTCTTGGA 
ATCTCTATCA TGATCAAGAA GCCTCAGAAG TCCAAACCAG GAGTGTTTTC CTTTCTTGAT 
CCTTTAGCCT ATGAGATCTG GATGTGCATT GTGTTTGCCT ACATTGGGGT CAGTGTAGTT 
TTATTCCTGG TCAGCAGATT TAGCCCCTAC GAGTGGCACA CTGAGGAATT TGAAGATGGA 
AGAGAAACAC AAAGTAGTGA ATCAACTAAT GAATTTGGGA TTTTTAATAG TCTCTGGTTT 
TCCTTGGGTG CCTTTATGCG GCAAGGATGC GATATTTCGC CAAGATCCCT CTCTGGGCGC 
ATTGTTGGAG GTGTGTGGTG GTTCTTTACC CTGATCATAA TCTCCTCCTA CACGGCTAAC 
TTAGCTGCCT TCCTGACTGT AGAGAGGATG GTGTCTCCCA TCGAAAGTGC TGAGGATCTG 
TCTAAGCAAA CAGAAATTGC TTATGGAACA TTAGACTCTG GCTCCACTAA AGAGTTTTTC 
AGGAGATCTA AAATCGCAGT GTTTGATAAA ATGTGGACTT ATATGAGGAG TGCAGAGCCC 
TCTGTGTTTG TGAGGACTAC CGCAGAAGGA GTAGCCAGAG TCCGGAAATC CAAAGGAAAG 
TATGCCTACT TGCTGGAGTC CACAATGAAC GAGTACATCG AGCAGAGGAA GCCTTGTGAC 
ACCATGAAAG TGGGAGGAAA CTTGGATTCC AAAGGCTACG GCATCGCCAC ACCTAAAGGA 
TCCTCATTAG GAACCCCAGT AAATCTTGCA GTATTGAAAC TCAGTGAGCA AGGCGTCTTA 
GACAAGCTGA AAAACAAATG GTGGTACGAT AAAGGTGAAT GTGGAGCCAA GGACTCGGGA 
AGTAAGGAAA AGACCAGTGC CCTCAGTCTG AGCAACGTTG CTGGAGTATT CTACATCCTT 
GTCGGGGGCC TTGGTTTGGC AATGCTGGTG GCTTTGATTG AGTTCTGTTA CAAGTCAAGG 
GCCGAGGCGA AACGAATGAA GGTGGCAAAG AATCCACAGA ATATTAACCC ATCTTCCTCG 
CAGAATTCCC AGAATTTTGC AACTTATAAG GAAGGTTACA ACGTATATGG CATCGAGAGT 
GTTAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058957.1
CDS	431..3082
Translation	MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI

Target ORF information:

RefSeq Version	NM_017261.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017261.2

ORF Insert Sequence:

ATGCAAAAGA TTATGCATAT TTCTGTCCTC CTTTCTCCTG TTTTATGGGG ACTGATTTTT 
GGTGTCTCTT CTAACAGCAT ACAGATAGGG GGGCTATTTC CAAGGGGCGC TGATCAAGAA 
TACAGTGCAT TTCGGGTAGG GATGGTTCAG TTTTCCACTT CGGAGTTCAG ACTGACACCC 
CATATCGACA ATTTGGAGGT AGCCAACAGT TTCGCAGTCA CCAATGCTTT CTGCTCCCAG 
TTTTCAAGAG GAGTCTACGC AATTTTTGGA TTTTATGACA AGAAGTCTGT AAATACCATC 
ACATCATTCT GTGGGACACT CCATGTGTCC TTCATCACAC CTAGCTTCCC AACAGATGGC 
ACACATCCAT TTGTCATCCA GATGCGACCT GACCTCAAAG GAGCACTCCT TAGCTTGATT 
GAGTACTACC AATGGGACAA GTTCGCATAC CTCTATGACA GTGACAGAGG CTTATCAACA 
CTGCAAGCTG TTCTGGATTC TGCTGCAGAG AAGAAGTGGC AGGTGACTGC TATCAATGTG 
GGGAACATCA ACAATGACAA GAAAGATGAG ACCTACAGAT CGCTCTTTCA AGATCTGGAG 
TTAAAAAAAG AACGGCGTGT AATCCTGGAC TGTGAAAGGG ATAAAGTAAA TGACATTGTG 
GACCAGGTTA TTACCATTGG AAAACATGTT AAAGGGTACC ATTATATCAT TGCAAATCTG 
GGATTCACTG ATGGGGACCT GCTGAAAATT CAGTTTGGAG GAGCAAATGT CTCTGGATTT 
CAGATTGTAG ACTACGACGA TTCCCTGGTG TCTAAATTTA TAGAAAGATG GTCAACACTG 
GAAGAGAAAG AATACCCTGG AGCACACACA GCGACAATTA AGTATACTTC GGCCCTGACG 
TATGATGCTG TCCAAGTGAT GACTGAAGCA TTCCGTAACC TTCGGAAGCA GAGGATTGAA 
ATATCCCGGA GAGGAAATGC AGGAGATTGT TTGGCCAACC CAGCTGTGCC CTGGGGACAA 
GGGGTCGAAA TAGAAAGGGC CCTCAAGCAG GTTCAAGTTG AAGGCCTCTC TGGAAATATA 
AAGTTTGACC AGAATGGAAA ACGAATAAAC TACACAATTA ACATCATGGA GCTCAAAACA 
AATGGACCCC GGAAGATTGG GTACTGGAGT GAAGTGGATA AAATGGTTGT CACCCTAACT 
GAGCTCCCAT CAGGAAATGA CACGTCTGGG CTTGAAAACA AGACTGTGGT GGTCACCACA 
ATATTGGAAT CTCCATATGT TATGATGAAG AAAAATCATG AAATGCTTGA AGGGAATGAG 
CGTTACGAGG GCTACTGTGT TGACTTAGCT GCAGAAATTG CTAAACACTG TGGGTTCAAG 
TACAAGCTGA CTATTGTTGG GGATGGCAAG TATGGGGCCA GGGATGCCGA CACCAAAATT 
TGGAATGGTA TGGTTGGAGA GCTTGTCTAC GGGAAAGCTG ACATTGCAAT TGCTCCATTA 
ACTATCACTC TCGTGAGAGA AGAGGTGATT GACTTCTCCA AGCCCTTCAT GAGTCTTGGA 
ATCTCTATCA TGATCAAGAA GCCTCAGAAG TCCAAACCAG GAGTGTTTTC CTTTCTTGAT 
CCTTTAGCCT ATGAGATCTG GATGTGCATT GTGTTTGCCT ACATTGGGGT CAGTGTAGTT 
TTATTCCTGG TCAGCAGATT TAGCCCCTAC GAGTGGCACA CTGAGGAATT TGAAGATGGA 
AGAGAAACAC AAAGTAGTGA ATCAACTAAT GAATTTGGGA TTTTTAATAG TCTCTGGTTT 
TCCTTGGGTG CCTTTATGCG GCAAGGATGC GATATTTCGC CAAGATCCCT CTCTGGGCGC 
ATTGTTGGAG GTGTGTGGTG GTTCTTTACC CTGATCATAA TCTCCTCCTA CACGGCTAAC 
TTAGCTGCCT TCCTGACTGT AGAGAGGATG GTGTCTCCCA TCGAAAGTGC TGAGGATCTG 
TCTAAGCAAA CAGAAATTGC TTATGGAACA TTAGACTCTG GCTCCACTAA AGAGTTTTTC 
AGGAGATCTA AAATCGCAGT GTTTGATAAA ATGTGGACTT ATATGAGGAG TGCAGAGCCC 
TCTGTGTTTG TGAGGACTAC CGCAGAAGGA GTAGCCAGAG TCCGGAAATC CAAAGGAAAG 
TATGCCTACT TGCTGGAGTC CACAATGAAC GAGTACATCG AGCAGAGGAA GCCTTGTGAC 
ACCATGAAAG TGGGAGGAAA CTTGGATTCC AAAGGCTACG GCATCGCCAC ACCTAAAGGA 
TCCTCATTAG GAACCCCAGT AAATCTTGCA GTATTGAAAC TCAGTGAGCA AGGCGTCTTA 
GACAAGCTGA AAAACAAATG GTGGTACGAT AAAGGTGAAT GTGGAGCCAA GGACTCGGGA 
AGTAAGGAAA AGACCAGTGC CCTCAGTCTG AGCAACGTTG CTGGAGTATT CTACATCCTT 
GTCGGGGGCC TTGGTTTGGC AATGCTGGTG GCTTTGATTG AGTTCTGTTA CAAGTCAAGG 
GCCGAGGCGA AACGAATGAA GGTGGCAAAG AATCCACAGA ATATTAACCC ATCTTCCTCG 
CAGAATTCCC AGAATTTTGC AACTTATAAG GAAGGTTACA ACGTATATGG CATCGAGAGT 
GTTAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria2 ( NM_001083811.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001077280.1 Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa13212
Clone ID Related Accession (Same CDS sequence)	NM_001083811.1
Accession Version	NM_001083811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor 2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M38061.1, AF164344.1, CO400088.1, M85035.1 and AABR07072744.1. Transcript Variant: This variant (2) uses an alternate exon in the 3' coding region compared to transcript variant 1, and encodes an isoform (2, also known as the flop isoform) that is the same length as isoform 1, but with few amino acid differences. RNA editing changes Gln607Arg (CAG->CGG) and Arg764Gly (AGA->GGA). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M85035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 7992055, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAGA TTATGCATAT TTCTGTCCTC CTTTCTCCTG TTTTATGGGG ACTGATTTTT 
GGTGTCTCTT CTAACAGCAT ACAGATAGGG GGGCTATTTC CAAGGGGCGC TGATCAAGAA 
TACAGTGCAT TTCGGGTAGG GATGGTTCAG TTTTCCACTT CGGAGTTCAG ACTGACACCC 
CATATCGACA ATTTGGAGGT AGCCAACAGT TTCGCAGTCA CCAATGCTTT CTGCTCCCAG 
TTTTCAAGAG GAGTCTACGC AATTTTTGGA TTTTATGACA AGAAGTCTGT AAATACCATC 
ACATCATTCT GTGGGACACT CCATGTGTCC TTCATCACAC CTAGCTTCCC AACAGATGGC 
ACACATCCAT TTGTCATCCA GATGCGACCT GACCTCAAAG GAGCACTCCT TAGCTTGATT 
GAGTACTACC AATGGGACAA GTTCGCATAC CTCTATGACA GTGACAGAGG CTTATCAACA 
CTGCAAGCTG TTCTGGATTC TGCTGCAGAG AAGAAGTGGC AGGTGACTGC TATCAATGTG 
GGGAACATCA ACAATGACAA GAAAGATGAG ACCTACAGAT CGCTCTTTCA AGATCTGGAG 
TTAAAAAAAG AACGGCGTGT AATCCTGGAC TGTGAAAGGG ATAAAGTAAA TGACATTGTG 
GACCAGGTTA TTACCATTGG AAAACATGTT AAAGGGTACC ATTATATCAT TGCAAATCTG 
GGATTCACTG ATGGGGACCT GCTGAAAATT CAGTTTGGAG GAGCAAATGT CTCTGGATTT 
CAGATTGTAG ACTACGACGA TTCCCTGGTG TCTAAATTTA TAGAAAGATG GTCAACACTG 
GAAGAGAAAG AATACCCTGG AGCACACACA GCGACAATTA AGTATACTTC GGCCCTGACG 
TATGATGCTG TCCAAGTGAT GACTGAAGCA TTCCGTAACC TTCGGAAGCA GAGGATTGAA 
ATATCCCGGA GAGGAAATGC AGGAGATTGT TTGGCCAACC CAGCTGTGCC CTGGGGACAA 
GGGGTCGAAA TAGAAAGGGC CCTCAAGCAG GTTCAAGTTG AAGGCCTCTC TGGAAATATA 
AAGTTTGACC AGAATGGAAA ACGAATAAAC TACACAATTA ACATCATGGA GCTCAAAACA 
AATGGACCCC GGAAGATTGG GTACTGGAGT GAAGTGGATA AAATGGTTGT CACCCTAACT 
GAGCTCCCAT CAGGAAATGA CACGTCTGGG CTTGAAAACA AGACTGTGGT GGTCACCACA 
ATATTGGAAT CTCCATATGT TATGATGAAG AAAAATCATG AAATGCTTGA AGGGAATGAG 
CGTTACGAGG GCTACTGTGT TGACTTAGCT GCAGAAATTG CTAAACACTG TGGGTTCAAG 
TACAAGCTGA CTATTGTTGG GGATGGCAAG TATGGGGCCA GGGATGCCGA CACCAAAATT 
TGGAATGGTA TGGTTGGAGA GCTTGTCTAC GGGAAAGCTG ACATTGCAAT TGCTCCATTA 
ACTATCACTC TCGTGAGAGA AGAGGTGATT GACTTCTCCA AGCCCTTCAT GAGTCTTGGA 
ATCTCTATCA TGATCAAGAA GCCTCAGAAG TCCAAACCAG GAGTGTTTTC CTTTCTTGAT 
CCTTTAGCCT ATGAGATCTG GATGTGCATT GTGTTTGCCT ACATTGGGGT CAGTGTAGTT 
TTATTCCTGG TCAGCAGATT TAGCCCCTAC GAGTGGCACA CTGAGGAATT TGAAGATGGA 
AGAGAAACAC AAAGTAGTGA ATCAACTAAT GAATTTGGGA TTTTTAATAG TCTCTGGTTT 
TCCTTGGGTG CCTTTATGCG GCAAGGATGC GATATTTCGC CAAGATCCCT CTCTGGGCGC 
ATTGTTGGAG GTGTGTGGTG GTTCTTTACC CTGATCATAA TCTCCTCCTA CACGGCTAAC 
TTAGCTGCCT TCCTGACTGT AGAGAGGATG GTGTCTCCCA TCGAAAGTGC TGAGGATCTG 
TCTAAGCAAA CAGAAATTGC TTATGGAACA TTAGACTCTG GCTCCACTAA AGAGTTTTTC 
AGGAGATCTA AAATCGCAGT GTTTGATAAA ATGTGGACTT ATATGAGGAG TGCAGAGCCC 
TCTGTGTTTG TGAGGACTAC CGCAGAAGGA GTAGCCAGAG TCCGGAAATC CAAAGGAAAG 
TATGCCTACT TGCTGGAGTC CACAATGAAC GAGTACATCG AGCAGAGGAA GCCTTGTGAC 
ACCATGAAAG TGGGAGGAAA CTTGGATTCC AAAGGCTACG GCATCGCCAC ACCTAAAGGA 
TCCTCATTAG GAAATGCGGT TAACCTCGCA GTACTAAAAC TGAATGAACA AGGCCTGTTG 
GACAAATTGA AAAACAAATG GTGGTACGAC AAAGGAGAGT GCGGCAGCGG GGGAGGTGAT 
TCCAAGGAAA AGACCAGTGC CCTCAGTCTG AGCAACGTTG CTGGAGTATT CTACATCCTT 
GTCGGGGGCC TTGGTTTGGC AATGCTGGTG GCTTTGATTG AGTTCTGTTA CAAGTCAAGG 
GCCGAGGCGA AACGAATGAA GGTGGCAAAG AATCCACAGA ATATTAACCC ATCTTCCTCG 
CAGAATTCCC AGAATTTTGC AACTTATAAG GAAGGTTACA ACGTATATGG CATCGAGAGT 
GTTAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077280.1
CDS	431..3082
Translation	MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI

Target ORF information:

RefSeq Version	NM_001083811.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor AMPA type subunit 2 (Gria2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083811.1

ORF Insert Sequence:

ATGCAAAAGA TTATGCATAT TTCTGTCCTC CTTTCTCCTG TTTTATGGGG ACTGATTTTT 
GGTGTCTCTT CTAACAGCAT ACAGATAGGG GGGCTATTTC CAAGGGGCGC TGATCAAGAA 
TACAGTGCAT TTCGGGTAGG GATGGTTCAG TTTTCCACTT CGGAGTTCAG ACTGACACCC 
CATATCGACA ATTTGGAGGT AGCCAACAGT TTCGCAGTCA CCAATGCTTT CTGCTCCCAG 
TTTTCAAGAG GAGTCTACGC AATTTTTGGA TTTTATGACA AGAAGTCTGT AAATACCATC 
ACATCATTCT GTGGGACACT CCATGTGTCC TTCATCACAC CTAGCTTCCC AACAGATGGC 
ACACATCCAT TTGTCATCCA GATGCGACCT GACCTCAAAG GAGCACTCCT TAGCTTGATT 
GAGTACTACC AATGGGACAA GTTCGCATAC CTCTATGACA GTGACAGAGG CTTATCAACA 
CTGCAAGCTG TTCTGGATTC TGCTGCAGAG AAGAAGTGGC AGGTGACTGC TATCAATGTG 
GGGAACATCA ACAATGACAA GAAAGATGAG ACCTACAGAT CGCTCTTTCA AGATCTGGAG 
TTAAAAAAAG AACGGCGTGT AATCCTGGAC TGTGAAAGGG ATAAAGTAAA TGACATTGTG 
GACCAGGTTA TTACCATTGG AAAACATGTT AAAGGGTACC ATTATATCAT TGCAAATCTG 
GGATTCACTG ATGGGGACCT GCTGAAAATT CAGTTTGGAG GAGCAAATGT CTCTGGATTT 
CAGATTGTAG ACTACGACGA TTCCCTGGTG TCTAAATTTA TAGAAAGATG GTCAACACTG 
GAAGAGAAAG AATACCCTGG AGCACACACA GCGACAATTA AGTATACTTC GGCCCTGACG 
TATGATGCTG TCCAAGTGAT GACTGAAGCA TTCCGTAACC TTCGGAAGCA GAGGATTGAA 
ATATCCCGGA GAGGAAATGC AGGAGATTGT TTGGCCAACC CAGCTGTGCC CTGGGGACAA 
GGGGTCGAAA TAGAAAGGGC CCTCAAGCAG GTTCAAGTTG AAGGCCTCTC TGGAAATATA 
AAGTTTGACC AGAATGGAAA ACGAATAAAC TACACAATTA ACATCATGGA GCTCAAAACA 
AATGGACCCC GGAAGATTGG GTACTGGAGT GAAGTGGATA AAATGGTTGT CACCCTAACT 
GAGCTCCCAT CAGGAAATGA CACGTCTGGG CTTGAAAACA AGACTGTGGT GGTCACCACA 
ATATTGGAAT CTCCATATGT TATGATGAAG AAAAATCATG AAATGCTTGA AGGGAATGAG 
CGTTACGAGG GCTACTGTGT TGACTTAGCT GCAGAAATTG CTAAACACTG TGGGTTCAAG 
TACAAGCTGA CTATTGTTGG GGATGGCAAG TATGGGGCCA GGGATGCCGA CACCAAAATT 
TGGAATGGTA TGGTTGGAGA GCTTGTCTAC GGGAAAGCTG ACATTGCAAT TGCTCCATTA 
ACTATCACTC TCGTGAGAGA AGAGGTGATT GACTTCTCCA AGCCCTTCAT GAGTCTTGGA 
ATCTCTATCA TGATCAAGAA GCCTCAGAAG TCCAAACCAG GAGTGTTTTC CTTTCTTGAT 
CCTTTAGCCT ATGAGATCTG GATGTGCATT GTGTTTGCCT ACATTGGGGT CAGTGTAGTT 
TTATTCCTGG TCAGCAGATT TAGCCCCTAC GAGTGGCACA CTGAGGAATT TGAAGATGGA 
AGAGAAACAC AAAGTAGTGA ATCAACTAAT GAATTTGGGA TTTTTAATAG TCTCTGGTTT 
TCCTTGGGTG CCTTTATGCG GCAAGGATGC GATATTTCGC CAAGATCCCT CTCTGGGCGC 
ATTGTTGGAG GTGTGTGGTG GTTCTTTACC CTGATCATAA TCTCCTCCTA CACGGCTAAC 
TTAGCTGCCT TCCTGACTGT AGAGAGGATG GTGTCTCCCA TCGAAAGTGC TGAGGATCTG 
TCTAAGCAAA CAGAAATTGC TTATGGAACA TTAGACTCTG GCTCCACTAA AGAGTTTTTC 
AGGAGATCTA AAATCGCAGT GTTTGATAAA ATGTGGACTT ATATGAGGAG TGCAGAGCCC 
TCTGTGTTTG TGAGGACTAC CGCAGAAGGA GTAGCCAGAG TCCGGAAATC CAAAGGAAAG 
TATGCCTACT TGCTGGAGTC CACAATGAAC GAGTACATCG AGCAGAGGAA GCCTTGTGAC 
ACCATGAAAG TGGGAGGAAA CTTGGATTCC AAAGGCTACG GCATCGCCAC ACCTAAAGGA 
TCCTCATTAG GAAATGCGGT TAACCTCGCA GTACTAAAAC TGAATGAACA AGGCCTGTTG 
GACAAATTGA AAAACAAATG GTGGTACGAC AAAGGAGAGT GCGGCAGCGG GGGAGGTGAT 
TCCAAGGAAA AGACCAGTGC CCTCAGTCTG AGCAACGTTG CTGGAGTATT CTACATCCTT 
GTCGGGGGCC TTGGTTTGGC AATGCTGGTG GCTTTGATTG AGTTCTGTTA CAAGTCAAGG 
GCCGAGGCGA AACGAATGAA GGTGGCAAAG AATCCACAGA ATATTAACCC ATCTTCCTCG 
CAGAATTCCC AGAATTTTGC AACTTATAAG GAAGGTTACA ACGTATATGG CATCGAGAGT 
GTTAAAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Gria2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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