Spred1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spred1
Entrez Gene ID	296072
Full Name	sprouty-related, EVH1 domain containing 1
General protein information	Preferred Names sprouty-related, EVH1 domain containing 1 Names sprouty-related, EVH1 domain-containing protein 1 sprouty protein with EVH-1 domain 1, related sequence
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762089.2	XP_008760311.1	sprouty-related, EVH1 domain-containing protein 1 isoform X1
NM_001047089.1	NP_001040554.1	sprouty-related, EVH1 domain-containing protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPRED1	XP_544609.3
Rattus norvegicus (Norway rat)	Spred1	NP_001040554.1
Xenopus tropicalis (tropical clawed frog)	spred1	NP_001008037.1
Gallus gallus (chicken)	SPRED1	NP_001186638.1
Pan troglodytes (chimpanzee)	SPRED1	XP_001138171.1
Bos taurus (cattle)	SPRED1	NP_001179445.1
Mus musculus (house mouse)	Spred1	NP_277059.1
Danio rerio (zebrafish)	spred1	NP_998397.1
Homo sapiens (human)	SPRED1	NP_689807.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-126-3p Promotes Matrix-Dependent Perivascular Cell Attachment, Migration and Intercellular Interaction.
Pitzler L, Auler M, Probst K, Frie C, Bergmeier V, Holzer T, Belluoccio D, van den Bergen J, Etich J, Ehlen H, Zhou Z, Bielke W, P?schl E, Paulsson M, Brachvogel B
Stem cells (Dayton, Ohio)34(5)1297-309(2016 05)

Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.
Friedmacher F, Gosemann JH, Fujiwara N, Takahashi H, Hofmann A, Puri P
Pediatric surgery international29(11)1193-8(2013 Nov)

MicroRNA-1 enhances the angiogenic differentiation of human cardiomyocyte progenitor cells.
van Mil A, Vrijsen KR, Goumans MJ, Metz CH, Doevendans PA, Sluijter JP
Journal of molecular medicine (Berlin, Germany)91(8)1001-12(2013 Aug)

Loss of angiomiR-126 and 130a in angiogenic early outgrowth cells from patients with chronic heart failure: role for impaired in vivo neovascularization and cardiac repair capacity.
Jakob P, Doerries C, Briand S, Mocharla P, Kr?nkel N, Besler C, Mueller M, Manes C, Templin C, Baltes C, Rudin M, Adams H, Wolfrum M, Noll G, Ruschitzka F, L?scher TF, Landmesser U
Circulation126(25)2962-75(2012 Dec)

Sprouty-related Ena/vasodilator-stimulated phosphoprotein homology 1-domain-containing protein (SPRED1), a tyrosine-protein phosphatase non-receptor type 11 (SHP2) substrate in the Ras/extracellular signal-regulated kinase (ERK) pathway.
Quintanar-Audelo M, Yusoff P, Sinniah S, Chandramouli S, Guy GR
The Journal of biological chemistry286(26)23102-12(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased expression of Sproutys and SPREDs during the terminal pseudoglandular stage may disrupt lung branching morphogenesis by interfering with epithelial-mesenchymal interactions contributing to PH.
Title: Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.

SPRED1 is a likely substrate of SHP2, whose tyrosine dephosphorylation is required to attenuate the inhibitory action of SPRED1 in the Ras/ERK pathway.
Title: Sprouty-related Ena/vasodilator-stimulated phosphoprotein homology 1-domain-containing protein (SPRED1), a tyrosine-protein phosphatase non-receptor type 11 (SHP2) substrate in the Ras/extracellular signal-regulated kinase (ERK) pathway.

The following Spred1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spred1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09185	NM_001047089.1 Latest version!	Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa45346	XM_008762089.2 Latest version!	Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spred1 ( NM_001047089.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001040554.1 Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), mRNA.

Price & Availability↑

CloneID	ORa09185
Clone ID Related Accession (Same CDS sequence)	NM_001047089.1
Accession Version	NM_001047089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-12
Organism	Rattus norvegicus(Norway rat)
Product	sprouty-related, EVH1 domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB121675.1. On or before Sep 7, 2006 this sequence version replaced XM_230454.4, XM_001080680.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB121675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
ATTGTCACTG TCTTCAGAGT CCCTCAGCAG GAAGAGAATG GCTGTGCAGA CTTTTATATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACTTTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAAGATATTT CTCAAGGGTG CCCAGCATCA AAAACTGAGA CTGAAGGAGG AGATGATGAT 
TTACAAACAA CTGAGGAGGA CACTTCCCGT TCCCTGGTGA AAGATCACTT TTTTCAGCAA 
GAGACAGTTG TTACCTGTGA TCCTTACAGA AGCTCAGACA TAAGACCATT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTGA GCCAGATACC ATTTTGTCAG 
CAAGGCCTAG ACATACAGAG CCGAAGTATG GAATATGTAC AGCGGCAGAT ATCTAAGGAA 
TGTGGGAGCC TCAAGTCCCA AGCTAGGGTC CCCTTGAAGT CTATCAGACA TGTCAGCTTT 
CAAGATGAGG ATGAGATTGT TAGAATAAAT CCTCGAGATA TCTTAATACG TCGGTATGCA 
GACTACAGAC ATCCGGACAT GTGGAAAAAT GACTTGGAGA GAGATGATAC TGACTCCAGT 
GTTCCATTTT CTAAACAAGA CAGTAAAAAG TCAGACTATT TCTACCACTG TGGGGATGAG 
ACTAAGTTAA GTTCTCTGAA AGACTCTGTG GTATTTAAGA CACAGCCTCC CTCATTAAAA 
TTTAAGTCAA AACGAAGAAA AGAGGATGGG GAACGTTCTC GCTGCGTGTA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGGCAGGGGG AAATGCCAGG ATGCTCCAGA TCCTGTTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCCGAGA GCATGCTGTA TCATTGTATG 
TCAGACTCAG AGGGAGACTT TTCTGACCCT TGTTCGTGTG ACACTAGCGA TGACAAGTTC 
TGCCTAAGGT GGTTAGCCCT GGTAGCTCTG TCTTTCATTG TACCGTGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GGTGCTGTGG TGGGAAACAC 
AAGGCTGCTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040554.1
CDS	1..1335
Translation	MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSIVTVFRVPQQEENGCADFYIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPASKTETEGGDDDLQTTEEDTSRSLVKDHFFQQETVVTCDPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFCQQGLDIQSRSMEYVQRQISKECGSLKSQARVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSSVPFSKQDSKKSDYFYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENGRGKCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	NM_001047089.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047089.1

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
ATTGTCACTG TCTTCAGAGT CCCTCAGCAG GAAGAGAATG GCTGTGCAGA CTTTTATATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACTTTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAAGATATTT CTCAAGGGTG CCCAGCATCA AAAACTGAGA CTGAAGGAGG AGATGATGAT 
TTACAAACAA CTGAGGAGGA CACTTCCCGT TCCCTGGTGA AAGATCACTT TTTTCAGCAA 
GAGACAGTTG TTACCTGTGA TCCTTACAGA AGCTCAGACA TAAGACCATT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTGA GCCAGATACC ATTTTGTCAG 
CAAGGCCTAG ACATACAGAG CCGAAGTATG GAATATGTAC AGCGGCAGAT ATCTAAGGAA 
TGTGGGAGCC TCAAGTCCCA AGCTAGGGTC CCCTTGAAGT CTATCAGACA TGTCAGCTTT 
CAAGATGAGG ATGAGATTGT TAGAATAAAT CCTCGAGATA TCTTAATACG TCGGTATGCA 
GACTACAGAC ATCCGGACAT GTGGAAAAAT GACTTGGAGA GAGATGATAC TGACTCCAGT 
GTTCCATTTT CTAAACAAGA CAGTAAAAAG TCAGACTATT TCTACCACTG TGGGGATGAG 
ACTAAGTTAA GTTCTCTGAA AGACTCTGTG GTATTTAAGA CACAGCCTCC CTCATTAAAA 
TTTAAGTCAA AACGAAGAAA AGAGGATGGG GAACGTTCTC GCTGCGTGTA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGGCAGGGGG AAATGCCAGG ATGCTCCAGA TCCTGTTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCCGAGA GCATGCTGTA TCATTGTATG 
TCAGACTCAG AGGGAGACTT TTCTGACCCT TGTTCGTGTG ACACTAGCGA TGACAAGTTC 
TGCCTAAGGT GGTTAGCCCT GGTAGCTCTG TCTTTCATTG TACCGTGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GGTGCTGTGG TGGGAAACAC 
AAGGCTGCTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred1 ( XM_008762089.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760311.1 Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45346
Clone ID Related Accession (Same CDS sequence)	XM_008762089.2
Accession Version	XM_008762089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC TAATTCCCAA TCTTTGCATC TGTTTTAGTA ATAGTTATGC ACGAGTGCGA 
GCTGTGGTGA TGACCAGAGA TGACTCAAGT GGTGGATGGC TACCACTCGG AGGGAGCGGA 
CTGAGCATTG TCACTGTCTT CAGAGTCCCT CAGCAGGAAG AGAATGGCTG TGCAGACTTT 
TATATCCGTG GAGAGAGACT CAGAGACAAA ATGGTGGTTT TGGAATGTAT GCTTAAGAAA 
GACCTCATCT ATAATAAGGT CACTCCCACT TTTCACCACT GGAAGATCGA TGACAAGAAG 
TTTGGCCTTA CCTTTCAGAG TCCTGCTGAT GCCAGGGCTT TTGATCGAGG CATTCGAAGA 
GCTATAGAAG ATATTTCTCA AGGGTGCCCA GCATCAAAAA CTGAGACTGA AGGAGGAGAT 
GATGATTTAC AAACAACTGA GGAGGACACT TCCCGTTCCC TGGTGAAAGA TCACTTTTTT 
CAGCAAGAGA CAGTTGTTAC CTGTGATCCT TACAGAAGCT CAGACATAAG ACCATTACCC 
TTTGAAGATC TGAATGCCAG AAGAGTCTAC TTGCAAAGCC AAGTGAGCCA GATACCATTT 
TGTCAGCAAG GCCTAGACAT ACAGAGCCGA AGTATGGAAT ATGTACAGCG GCAGATATCT 
AAGGAATGTG GGAGCCTCAA GTCCCAAGCT AGGGTCCCCT TGAAGTCTAT CAGACATGTC 
AGCTTTCAAG ATGAGGATGA GATTGTTAGA ATAAATCCTC GAGATATCTT AATACGTCGG 
TATGCAGACT ACAGACATCC GGACATGTGG AAAAATGACT TGGAGAGAGA TGATACTGAC 
TCCAGTGTTC CATTTTCTAA ACAAGACAGT AAAAAGTCAG ACTATTTCTA CCACTGTGGG 
GATGAGACTA AGTTAAGTTC TCTGAAAGAC TCTGTGGTAT TTAAGACACA GCCTCCCTCA 
TTAAAATTTA AGTCAAAACG AAGAAAAGAG GATGGGGAAC GTTCTCGCTG CGTGTACTGC 
CAGGAAAGGT TTAATCATGA AGAAAATGGC AGGGGGAAAT GCCAGGATGC TCCAGATCCT 
GTTAAAAGAT GCATATATCA AGTTAGTTGC ATGCTCTGTG CCGAGAGCAT GCTGTATCAT 
TGTATGTCAG ACTCAGAGGG AGACTTTTCT GACCCTTGTT CGTGTGACAC TAGCGATGAC 
AAGTTCTGCC TAAGGTGGTT AGCCCTGGTA GCTCTGTCTT TCATTGTACC GTGTATGTGC 
TGCTACGTCC CTTTGAGAAT GTGCCATCGC TGTGGTGAGG CATGTGGGTG CTGTGGTGGG 
AAACACAAGG CTGCTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760311.1
CDS	2286..3626
Translation	MEELIPNLCICFSNSYARVRAVVMTRDDSSGGWLPLGGSGLSIVTVFRVPQQEENGCADFYIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPASKTETEGGDDDLQTTEEDTSRSLVKDHFFQQETVVTCDPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFCQQGLDIQSRSMEYVQRQISKECGSLKSQARVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSSVPFSKQDSKKSDYFYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENGRGKCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	XM_008762089.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sprouty-related, EVH1 domain containing 1 (Spred1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762089.2

ORF Insert Sequence:

ATGGAAGAGC TAATTCCCAA TCTTTGCATC TGTTTTAGTA ATAGTTATGC ACGAGTGCGA 
GCTGTGGTGA TGACCAGAGA TGACTCAAGT GGTGGATGGC TACCACTCGG AGGGAGCGGA 
CTGAGCATTG TCACTGTCTT CAGAGTCCCT CAGCAGGAAG AGAATGGCTG TGCAGACTTT 
TATATCCGTG GAGAGAGACT CAGAGACAAA ATGGTGGTTT TGGAATGTAT GCTTAAGAAA 
GACCTCATCT ATAATAAGGT CACTCCCACT TTTCACCACT GGAAGATCGA TGACAAGAAG 
TTTGGCCTTA CCTTTCAGAG TCCTGCTGAT GCCAGGGCTT TTGATCGAGG CATTCGAAGA 
GCTATAGAAG ATATTTCTCA AGGGTGCCCA GCATCAAAAA CTGAGACTGA AGGAGGAGAT 
GATGATTTAC AAACAACTGA GGAGGACACT TCCCGTTCCC TGGTGAAAGA TCACTTTTTT 
CAGCAAGAGA CAGTTGTTAC CTGTGATCCT TACAGAAGCT CAGACATAAG ACCATTACCC 
TTTGAAGATC TGAATGCCAG AAGAGTCTAC TTGCAAAGCC AAGTGAGCCA GATACCATTT 
TGTCAGCAAG GCCTAGACAT ACAGAGCCGA AGTATGGAAT ATGTACAGCG GCAGATATCT 
AAGGAATGTG GGAGCCTCAA GTCCCAAGCT AGGGTCCCCT TGAAGTCTAT CAGACATGTC 
AGCTTTCAAG ATGAGGATGA GATTGTTAGA ATAAATCCTC GAGATATCTT AATACGTCGG 
TATGCAGACT ACAGACATCC GGACATGTGG AAAAATGACT TGGAGAGAGA TGATACTGAC 
TCCAGTGTTC CATTTTCTAA ACAAGACAGT AAAAAGTCAG ACTATTTCTA CCACTGTGGG 
GATGAGACTA AGTTAAGTTC TCTGAAAGAC TCTGTGGTAT TTAAGACACA GCCTCCCTCA 
TTAAAATTTA AGTCAAAACG AAGAAAAGAG GATGGGGAAC GTTCTCGCTG CGTGTACTGC 
CAGGAAAGGT TTAATCATGA AGAAAATGGC AGGGGGAAAT GCCAGGATGC TCCAGATCCT 
GTTAAAAGAT GCATATATCA AGTTAGTTGC ATGCTCTGTG CCGAGAGCAT GCTGTATCAT 
TGTATGTCAG ACTCAGAGGG AGACTTTTCT GACCCTTGTT CGTGTGACAC TAGCGATGAC 
AAGTTCTGCC TAAGGTGGTT AGCCCTGGTA GCTCTGTCTT TCATTGTACC GTGTATGTGC 
TGCTACGTCC CTTTGAGAAT GTGCCATCGC TGTGGTGAGG CATGTGGGTG CTGTGGTGGG 
AAACACAAGG CTGCTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-126-3p Promotes Matrix-Dependent Perivascular Cell Attachment, Migration and Intercellular Interaction.
Stem cells (Dayton, Ohio)34(5)1297-309(2016 05)
Pitzler L,Auler M,Probst K,Frie C,Bergmeier V,Holzer T,Belluoccio D,van den Bergen J,Etich J,Ehlen H,Zhou Z,Bielke W,P?schl E,Paulsson M,Brachvogel B

Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.
Pediatric surgery international29(11)1193-8(2013 Nov)
Friedmacher F,Gosemann JH,Fujiwara N,Takahashi H,Hofmann A,Puri P

MicroRNA-1 enhances the angiogenic differentiation of human cardiomyocyte progenitor cells.
Journal of molecular medicine (Berlin, Germany)91(8)1001-12(2013 Aug)
van Mil A,Vrijsen KR,Goumans MJ,Metz CH,Doevendans PA,Sluijter JP

Loss of angiomiR-126 and 130a in angiogenic early outgrowth cells from patients with chronic heart failure: role for impaired in vivo neovascularization and cardiac repair capacity.
Circulation126(25)2962-75(2012 Dec)
Jakob P,Doerries C,Briand S,Mocharla P,Kr?nkel N,Besler C,Mueller M,Manes C,Templin C,Baltes C,Rudin M,Adams H,Wolfrum M,Noll G,Ruschitzka F,L?scher TF,Landmesser U

Sprouty-related Ena/vasodilator-stimulated phosphoprotein homology 1-domain-containing protein (SPRED1), a tyrosine-protein phosphatase non-receptor type 11 (SHP2) substrate in the Ras/extracellular signal-regulated kinase (ERK) pathway.
The Journal of biological chemistry286(26)23102-12(2011 Jul)
Quintanar-Audelo M,Yusoff P,Sinniah S,Chandramouli S,Guy GR

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Spred1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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