Gja8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gja8
Entrez Gene ID	29601
Full Name	gap junction protein, alpha 8
Synonyms	Cx50
General protein information	Preferred Names gap junction protein, alpha 8 Names gap junction alpha-8 protein connexin-50 gap junction membrane channel protein alpha 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	mutation responsible for the formation of cataracts [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153465.2	NP_703195.2	gap junction alpha-8 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GJA8	XP_524857.1
Mus musculus (house mouse)	Gja8	NP_032149.1
Rattus norvegicus (Norway rat)	Gja8	NP_703195.2
Homo sapiens (human)	GJA8	NP_005258.2
Danio rerio (zebrafish)	cx44.1	NP_571884.2
	GJA8	XP_001095298.1
Canis lupus familiaris (dog)	GJA8	XP_005630769.1
Bos taurus (cattle)	GJA8	XP_005204058.1
Gallus gallus (chicken)	GJA8	NP_990328.1
Xenopus tropicalis (tropical clawed frog)	gja8	XP_004918275.1

Gene Ontology
Bibliography

Related articles in PubMed

Connexin 50 mutation lowers blood pressure in spontaneously hypertensive rat.
?eda O, Li?ka F, Pravenec M, Vernerov? Z, Kazdov? L, K?enov? D, Z?dek V, ?edov? L, Krupkov? M, K?en V
Physiological research66(1)15-28(2017 Mar)

Heterozygous connexin 50 mutation affects metabolic syndrome attributes in spontaneously hypertensive rat.
?eda O, K?enov? D, Oliyarnyk O, ?edov? L, Krupkov? M, Li?ka F, Chyl?kov? B, Kazdov? L, K?en V
Lipids in health and disease15(1)199(2016 Nov)

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.
Rodriguez-Jimenez FJ, Alastrue A, Stojkovic M, Erceg S, Moreno-Manzano V
Cell and tissue research365(2)295-307(2016 08)

Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.
Pinto BI, Garc?a IE, Pupo A, Retamal MA, Mart?nez AD, Latorre R, Gonz?lez C
The Journal of biological chemistry291(30)15740-52(2016 07)

Connexin 50 Expression in Ependymal Stem Progenitor Cells after Spinal Cord Injury Activation.
Rodriguez-Jimenez FJ, Alastrue-Agudo A, Stojkovic M, Erceg S, Moreno-Manzano V
International journal of molecular sciences16(11)26608-18(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The connexin 50 mutation lowers blood pressure in the spontaneously hypertensive rat.
Title: Connexin 50 mutation lowers blood pressure in spontaneously hypertensive rat.

Gja8 mutation L7Q in heterozygous state affects the lipid profile and the oxidative stress parameters in the spontaneously hypertensive rat strain.
Title: Heterozygous connexin 50 mutation affects metabolic syndrome attributes in spontaneously hypertensive rat.

Cx50 might constitute an interesting mechanism for Sox2 induction to modulate the endogenous regenerative potential of neuronal tissue with a potential application in regenerative therapies.
Title: Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.

the role of the charged residues at the end of TM-1 in voltage sensing in Cx26, Cx46, and Cx50.
Title: Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.

Upon ependymal stem cells differentiation, Cx50 expression favors glial cell fate, since higher expression levels, endogenous or by over-expression of Cx50, augmented the expression of the astrocyte marker GFAP and impaired the neuronal marker Tuj1.
Title: Connexin 50 Expression in Ependymal Stem Progenitor Cells after Spinal Cord Injury Activation.

The following Gja8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gja8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42644	NM_153465.2 Latest version!	Rattus norvegicus gap junction protein, alpha 8 (Gja8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gja8 ( NM_153465.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_703195.2 Rattus norvegicus gap junction protein, alpha 8 (Gja8), mRNA.

Price & Availability↑

CloneID	ORa42644
Clone ID Related Accession (Same CDS sequence)	NM_153465.2
Accession Version	NM_153465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	gap junction alpha-8 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU445788.1. On Jul 11, 2008 this sequence version replaced NM_153465.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGGCGACT GGAGTTTCCA GGGAAACATC TTGGAAGAGG TGAATGAACA TTCCACCGTC 
ATCGGCAGAG TTTGGCTCAC GGTGCTTTTC ATCTTCCGCA TCCTCATCCT TGGCACCGCA 
GCCGAGTTTG TGTGGGGCGA TGAGCAATCT GATTTTGTAT GCAACACCCA GCAGCCAGGT 
TGTGAGAACG TCTGCTATGA TGAGGCCTTT CCCATCTCGC ACATCCGCCT CTGGGTCCTG 
CAGATCATCT TCGTCTCCAC TCCATCGCTG ATGTACGTGG GGCACGCGGT ACACCACGTT 
CGCATGGAGG AGAAGCGAAA GGACCGTGAG GCTGAGGAGC TCTGTCAGCA GTCTCGCAGC 
AACGGGGGTG AGAGGGTACC AATCGCCCCA GACCAGGCCA GCATCAGGAA GAGCAGCAGC 
AGTAGCAAAG GCACCAAGAA GTTCCGGCTG GAGGGCACAC TGCTAAGGAC CTATGTCTGC 
CACATCATCT TCAAGACCCT CTTTGAGGTG GGCTTCATCG TGGGCCATTA CTTCCTGTAT 
GGTTTCCGCA TCCTGCCCCT CTATCGCTGC AGCCGGTGGC CCTGCCCCAA TGTGGTGGAC 
TGCTTTGTGT CCCGGCCTAC TGAGAAAACC ATCTTCATCC TCTTCATGTT GTCCGTTGCT 
TTTGTGTCCC TCTTCCTCAA CATCATGGAG ATGAGCCACC TGGGCATGAA GGGAATCCGG 
TCTGCCTTCA AGAGGCCTGC AGAGCAACCA CTGGGGGAGA TTGCCGAGAA GTCCCTCCAC 
TCCATTGCAG TTTCCTCCAT CCAGAAAGCC AAGGGCTACC AGCTCCTAGA AGAAGAGAAG 
ATCGTTTCAC ACTATTTCCC TTTGACGGAG GTTGGAATGG TGGAGACCAG CCCCCTGTCA 
GCCAAGCCTT TTAGTCAGTT TGAGGAGAAG ATCGGCACAG GACCCCTGGC AGATATGTCA 
CGGGGTTACC AAGAAACCCT GCCTTCTTAT GCTCAGGTGG GGGCCCAGGA AGTGGAGCGG 
GAAGAGCAGC CCGTAGAGGA GGCTGTGGAA CCGGAATTGG GAGAGAAGAA GCAAGAAGCA 
GAGAAGGTGG CCCCGGAAGG GCAGGAGACG GTTGCAGTGC CAGACGGGGA GAAAGTAGAG 
ACCCCTGGGG TAGGGAAGGA TGATGAGAAA GAAGAGCTGC AAGCTGAAAA GGTAACCAAG 
CAAGGGCTGT CTGCAGAGAA AGCGCCCACA CTCTGTCCAG AGCTGACAAC CGATGACAAC 
CGGCCCTTGA GCAGGCTGAG CAAAGCCAGC AGCAGGGCCA GGTCTGATGA TCTCACCATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703195.2
CDS	78..1400
Translation	MGDWSFQGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLMYVGHAVHHVRMEEKRKDREAEELCQQSRSNGGERVPIAPDQASIRKSSSSSKGTKKFRLEGTLLRTYVCHIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKTIFILFMLSVAFVSLFLNIMEMSHLGMKGIRSAFKRPAEQPLGEIAEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLSAKPFSQFEEKIGTGPLADMSRGYQETLPSYAQVGAQEVEREEQPVEEAVEPELGEKKQEAEKVAPEGQETVAVPDGEKVETPGVGKDDEKEELQAEKVTKQGLSAEKAPTLCPELTTDDNRPLSRLSKASSRARSDDLTI

Target ORF information:

RefSeq Version	NM_153465.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, alpha 8 (Gja8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153465.2

ORF Insert Sequence:

ATGGGCGACT GGAGTTTCCA GGGAAACATC TTGGAAGAGG TGAATGAACA TTCCACCGTC 
ATCGGCAGAG TTTGGCTCAC GGTGCTTTTC ATCTTCCGCA TCCTCATCCT TGGCACCGCA 
GCCGAGTTTG TGTGGGGCGA TGAGCAATCT GATTTTGTAT GCAACACCCA GCAGCCAGGT 
TGTGAGAACG TCTGCTATGA TGAGGCCTTT CCCATCTCGC ACATCCGCCT CTGGGTCCTG 
CAGATCATCT TCGTCTCCAC TCCATCGCTG ATGTACGTGG GGCACGCGGT ACACCACGTT 
CGCATGGAGG AGAAGCGAAA GGACCGTGAG GCTGAGGAGC TCTGTCAGCA GTCTCGCAGC 
AACGGGGGTG AGAGGGTACC AATCGCCCCA GACCAGGCCA GCATCAGGAA GAGCAGCAGC 
AGTAGCAAAG GCACCAAGAA GTTCCGGCTG GAGGGCACAC TGCTAAGGAC CTATGTCTGC 
CACATCATCT TCAAGACCCT CTTTGAGGTG GGCTTCATCG TGGGCCATTA CTTCCTGTAT 
GGTTTCCGCA TCCTGCCCCT CTATCGCTGC AGCCGGTGGC CCTGCCCCAA TGTGGTGGAC 
TGCTTTGTGT CCCGGCCTAC TGAGAAAACC ATCTTCATCC TCTTCATGTT GTCCGTTGCT 
TTTGTGTCCC TCTTCCTCAA CATCATGGAG ATGAGCCACC TGGGCATGAA GGGAATCCGG 
TCTGCCTTCA AGAGGCCTGC AGAGCAACCA CTGGGGGAGA TTGCCGAGAA GTCCCTCCAC 
TCCATTGCAG TTTCCTCCAT CCAGAAAGCC AAGGGCTACC AGCTCCTAGA AGAAGAGAAG 
ATCGTTTCAC ACTATTTCCC TTTGACGGAG GTTGGAATGG TGGAGACCAG CCCCCTGTCA 
GCCAAGCCTT TTAGTCAGTT TGAGGAGAAG ATCGGCACAG GACCCCTGGC AGATATGTCA 
CGGGGTTACC AAGAAACCCT GCCTTCTTAT GCTCAGGTGG GGGCCCAGGA AGTGGAGCGG 
GAAGAGCAGC CCGTAGAGGA GGCTGTGGAA CCGGAATTGG GAGAGAAGAA GCAAGAAGCA 
GAGAAGGTGG CCCCGGAAGG GCAGGAGACG GTTGCAGTGC CAGACGGGGA GAAAGTAGAG 
ACCCCTGGGG TAGGGAAGGA TGATGAGAAA GAAGAGCTGC AAGCTGAAAA GGTAACCAAG 
CAAGGGCTGT CTGCAGAGAA AGCGCCCACA CTCTGTCCAG AGCTGACAAC CGATGACAAC 
CGGCCCTTGA GCAGGCTGAG CAAAGCCAGC AGCAGGGCCA GGTCTGATGA TCTCACCATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Connexin 50 mutation lowers blood pressure in spontaneously hypertensive rat.
Physiological research66(1)15-28(2017 Mar)
?eda O,Li?ka F,Pravenec M,Vernerov? Z,Kazdov? L,K?enov? D,Z?dek V,?edov? L,Krupkov? M,K?en V

Heterozygous connexin 50 mutation affects metabolic syndrome attributes in spontaneously hypertensive rat.
Lipids in health and disease15(1)199(2016 Nov)
?eda O,K?enov? D,Oliyarnyk O,?edov? L,Krupkov? M,Li?ka F,Chyl?kov? B,Kazdov? L,K?en V

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.
Cell and tissue research365(2)295-307(2016 08)
Rodriguez-Jimenez FJ,Alastrue A,Stojkovic M,Erceg S,Moreno-Manzano V

Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.
The Journal of biological chemistry291(30)15740-52(2016 07)
Pinto BI,Garc?a IE,Pupo A,Retamal MA,Mart?nez AD,Latorre R,Gonz?lez C

Connexin 50 Expression in Ependymal Stem Progenitor Cells after Spinal Cord Injury Activation.
International journal of molecular sciences16(11)26608-18(2015 Nov)
Rodriguez-Jimenez FJ,Alastrue-Agudo A,Stojkovic M,Erceg S,Moreno-Manzano V

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Gja8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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