Atp2b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp2b1
Entrez Gene ID	29598
Full Name	ATPase plasma membrane Ca2+ transporting 1
Synonyms	Pmca1a,Pmca1b,Pmca1c
General protein information	Preferred Names ATPase plasma membrane Ca2+ transporting 1 Names plasma membrane calcium-transporting ATPase 1 ATPase, Ca++ transporting, plasma membrane 1 PMCA1 plasma membrane calcium ATPase plasma membrane calcium pump
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	regulates intracellular Ca2+ levels [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241296.3	XP_006241358.1	plasma membrane calcium-transporting ATPase 1 isoform X2
NM_053311.1	NP_445763.1	plasma membrane calcium-transporting ATPase 1
XM_006241294.3	XP_006241356.1	plasma membrane calcium-transporting ATPase 1 isoform X1
XM_006241295.3	XP_006241357.1	plasma membrane calcium-transporting ATPase 1 isoform X1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ACA4	NP_181687.1
Oryza sativa (rice)	Os01g0939100	NP_001045345.1
Homo sapiens (human)	ATP2B1	NP_001673.2
Gallus gallus (chicken)	ATP2B1	NP_001161474.1
Danio rerio (zebrafish)	atp2b1a	NP_001038222.1
Pan troglodytes (chimpanzee)	ATP2B1	XP_003952314.1
Canis lupus familiaris (dog)	ATP2B1	NP_001184013.1
Bos taurus (cattle)	ATP2B1	NP_777121.1
Arabidopsis thaliana (thale cress)	ACA11	NP_191292.1
Oryza sativa (rice)	Os05g0495600	NP_001055932.2
	ATP2B1	XP_001102031.1
Mus musculus (house mouse)	Atp2b1	NP_080758.1
Rattus norvegicus (Norway rat)	Atp2b1	NP_445763.1
Oryza sativa (rice)	Os12g0136900	NP_001066105.1

Gene Ontology
Bibliography

Related articles in PubMed

A complex of Neuroplastin and Plasma Membrane Ca
Korthals M, Langnaese K, Smalla KH, K?hne T, Herrera-Molina R, Handschuh J, Lehmann AC, Mamula D, Naumann M, Seidenbecher C, Zuschratter W, Tedford K, Gundelfinger ED, Montag D, Fischer KD, Thomas U
Scientific reports7(1)8358(2017 Aug)

ATP2B1 gene Silencing Increases Insulin Sensitivity through Facilitating Akt Activation via the Ca
Long Y, Xia JY, Chen SW, Gao CL, Liang GN, He XM, Wu J, Jiang CX, Liu X, Huang W, Wan Q, Xu Y
International journal of biological sciences13(9)1203-1212(2017)

Deletion of the intestinal plasma membrane calcium pump, isoform 1, Atp2b1, in mice is associated with decreased bone mineral density and impaired responsiveness to 1, 25-dihydroxyvitamin D3.
Ryan ZC, Craig TA, Filoteo AG, Westendorf JJ, Cartwright EJ, Neyses L, Strehler EE, Kumar R
Biochemical and biophysical research communications467(1)152-6(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Negative calcium balance in aged ovariectomized female rats is associated with downregulation of PMCA1b.
Title: Estrogen deficiency-induced Ca balance impairment is associated with decrease in expression of epithelial Ca transport proteins in aged female rats.

The contribution of PMCA after nerve injury in a rat model of neuropathic pain, is reported.
Title: Painful nerve injury increases plasma membrane Ca2+-ATPase activity in axotomized sensory neurons.

VDR knockdown results in a decrease in [Ca(2)]i in NRK cell lines. *
Title: Adenovirus-delivered microRNA targeting the vitamin D receptor reduces intracellular Ca?? concentrations by regulating the expression of Ca??-transport proteins in renal epithelial cells.

Data suggest that PMCA1a is a calcium pump specialized for neurons, where it may contribute to the modulation of somatic and dendritic Ca(2+) transients.
Title: Cellular and subcellular localization of the neuron-specific plasma membrane calcium ATPase PMCA1a in the rat brain.

Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance are reported.
Title: Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance.

The following Atp2b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp2b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12502	NM_053311.1 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa30089	XM_006241294.3 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa30089	XM_006241295.3 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa30090	XM_006241296.3 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp2b1 ( NM_053311.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445763.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), mRNA.

Price & Availability↑

CloneID	ORa12502
Clone ID Related Accession (Same CDS sequence)	NM_053311.1
Accession Version	NM_053311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03753.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GACGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTTA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCTGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATGT AACAAATGTT TCTACCCCTA CACATGTAGT GTTTTCCTCT 
TCTACTGCTT CTACTCCTGT GGGGTATCCG AGTGGTGAAT GCATTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445763.1
CDS	265..3795
Translation	MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQMDVVNAFQSGGSIQGALRRQPSIASQHHDVTNVSTPTHVVFSSSTASTPVGYPSGECIS

Target ORF information:

RefSeq Version	NM_053311.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053311.1

ORF Insert Sequence:

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GACGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTTA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCTGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATGT AACAAATGTT TCTACCCCTA CACATGTAGT GTTTTCCTCT 
TCTACTGCTT CTACTCCTGT GGGGTATCCG AGTGGTGAAT GCATTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b1 ( XM_006241294.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241356.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30089
Clone ID Related Accession (Same CDS sequence)	XM_006241294.3 , XM_006241295.3
Accession Version	XM_006241294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATAT CCGAGTGGTG AATGCATTTC GTAGCTCTTT ATACGAAGGG 
TTAGAGAAGC CAGAATCAAG AAGTTCAATC CACAACTTTA TGACACACCC CGAGTTTAGG 
ATAGAAGATT CAGAGCCGCA CATCCCCCTT ATCGATGACA CTGATGCTGA AGATGACGCT 
CCTACTAAAC GCAACTCCAG CCCTCCGCCC TCTCCTAACA AAAATAACAA TGCCGTTGAC 
AGCGGGATCC ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCGTC CCCAGGGAGC 
CCACTGCATA GTTTGGAAAC ATCACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241356.1
CDS	357..4106
Translation	MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQMDVVNAFQSGGSIQGALRRQPSIASQHHDIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSATSSSPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_006241294.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241294.3

ORF Insert Sequence:

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATAT CCGAGTGGTG AATGCATTTC GTAGCTCTTT ATACGAAGGG 
TTAGAGAAGC CAGAATCAAG AAGTTCAATC CACAACTTTA TGACACACCC CGAGTTTAGG 
ATAGAAGATT CAGAGCCGCA CATCCCCCTT ATCGATGACA CTGATGCTGA AGATGACGCT 
CCTACTAAAC GCAACTCCAG CCCTCCGCCC TCTCCTAACA AAAATAACAA TGCCGTTGAC 
AGCGGGATCC ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCGTC CCCAGGGAGC 
CCACTGCATA GTTTGGAAAC ATCACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b1 ( XM_006241295.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241357.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X2, mRNA.

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CloneID	ORa30089
Clone ID Related Accession (Same CDS sequence)	XM_006241294.3 , XM_006241295.3
Accession Version	XM_006241295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATAT CCGAGTGGTG AATGCATTTC GTAGCTCTTT ATACGAAGGG 
TTAGAGAAGC CAGAATCAAG AAGTTCAATC CACAACTTTA TGACACACCC CGAGTTTAGG 
ATAGAAGATT CAGAGCCGCA CATCCCCCTT ATCGATGACA CTGATGCTGA AGATGACGCT 
CCTACTAAAC GCAACTCCAG CCCTCCGCCC TCTCCTAACA AAAATAACAA TGCCGTTGAC 
AGCGGGATCC ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCGTC CCCAGGGAGC 
CCACTGCATA GTTTGGAAAC ATCACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241357.1
CDS	317..4066
Translation	MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQMDVVNAFQSGGSIQGALRRQPSIASQHHDIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSATSSSPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_006241295.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241295.3

ORF Insert Sequence:

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATGGATGTA 
GTGAATGCTT TCCAGAGTGG AGGTTCCATT CAGGGGGCTC TAAGGCGACA ACCCTCCATC 
GCCAGCCAGC ACCATGATAT CCGAGTGGTG AATGCATTTC GTAGCTCTTT ATACGAAGGG 
TTAGAGAAGC CAGAATCAAG AAGTTCAATC CACAACTTTA TGACACACCC CGAGTTTAGG 
ATAGAAGATT CAGAGCCGCA CATCCCCCTT ATCGATGACA CTGATGCTGA AGATGACGCT 
CCTACTAAAC GCAACTCCAG CCCTCCGCCC TCTCCTAACA AAAATAACAA TGCCGTTGAC 
AGCGGGATCC ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCGTC CCCAGGGAGC 
CCACTGCATA GTTTGGAAAC ATCACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b1 ( XM_006241296.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241358.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X3, mRNA.

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CloneID	ORa30090
Clone ID Related Accession (Same CDS sequence)	XM_006241296.3
Accession Version	XM_006241296.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATCCGAGTG 
GTGAATGCAT TTCGTAGCTC TTTATACGAA GGGTTAGAGA AGCCAGAATC AAGAAGTTCA 
ATCCACAACT TTATGACACA CCCCGAGTTT AGGATAGAAG ATTCAGAGCC GCACATCCCC 
CTTATCGATG ACACTGATGC TGAAGATGAC GCTCCTACTA AACGCAACTC CAGCCCTCCG 
CCCTCTCCTA ACAAAAATAA CAATGCCGTT GACAGCGGGA TCCACCTTAC AATAGAAATG 
AACAAGTCTG CTACCTCTTC GTCCCCAGGG AGCCCACTGC ATAGTTTGGA AACATCACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241358.1
CDS	357..4019
Translation	MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSATSSSPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_006241296.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 1 (Atp2b1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241296.3

ORF Insert Sequence:

ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATAGTGGTG TGAAAAACTC TTTGAAAGAA 
GCTAATCATG ATGGCGACTT TGGCATCACA CTTGCCGAGC TGCGGGCCCT CATGGAGCTC 
AGGTCCACAG ATGCATTACG AAAAATACAG GAAAGCTATG GGGATGTCTA TGGAATTTGC 
ACCAAGTTGA AAACATCTCC CAATGAAGGT TTAAGTGGAA ATCCTGCAGA TTTAGAAAGA 
AGAGAAGCAG TGTTTGGAAA GAACTTTATA CCTCCTAAGA AGCCAAAAAC CTTTCTTCAG 
TTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAC TAGAAATCGC AGCCATAGTA 
TCATTGGGCC TTTCTTTTTA TCAACCTCCG GAAGGGGATA ATGCACTTTG TGGAGAAGTT 
TCTGTTGGGG AAGAAGAAGG TGAAGGAGAA ACGGGGTGGA TTGAAGGAGC TGCCATCCTC 
TTGTCGGTGG TGTGTGTGGT GTTAGTGACG GCTTTCAATG ACTGGAGTAA GGAGAAACAG 
TTCCGAGGCT TGCAGAGTCG AATTGAACAG GAACAGAAGT TCACCGTCAT CAGGGGTGGG 
CAGGTCATCC AGATACCTGT AGCTGACATT ACTGTTGGAG ACATTGCCCA AGTGAAGTAC 
GGTGATCTTC TTCCAGCTGA TGGTATATTG ATTCAAGGCA ATGACCTTAA AATTGACGAG 
AGTTCGTTGA CTGGCGAGTC TGACCATGTT AAGAAGTCTC TAGATAAAGA CCCCTTGCTT 
CTTTCAGGTA CTCATGTAAT GGAAGGTTCT GGAAGGATGG TCGTTACTGC TGTAGGTGTG 
AACTCTCAAA CTGGGATCAT CTTTACCTTA CTTGGAGCTG GAGGCGAAGA GGAGGAAAAG 
AAAGATGAGA AGAAGAAAGA AAAGAAAAAT AAGAAACAAG ATGGAGCAAT TGAAAATCGC 
AACAAAGCAA AAGCCCAAGA CGGCGCGGCC ATGGAGATGC AGCCTCTGAA GAGTGAAGAA 
GGGGGAGATG GTGATGAGAA AGACAAGAAG AAAGCAAATC TGCCAAAGAA GGAAAAGTCT 
GTGTTACAAG GGAAACTTAC GAAGCTGGCT GTTCAGATTG GCAAAGCAGG TCTGCTGATG 
TCTGCCATCA CGGTTATCAT CCTAGTGCTA TATTTTGTGA TCGATACCTT CTGGGTTCAG 
AAGAGGCCGT GGCTTGCTGA GTGCACGCCA ATCTACATCC AGTACTTTGT GAAGTTCTTC 
ATTATCGGGG TCACCGTTCT AGTGGTCGCG GTGCCGGAAG GCCTCCCGCT GGCCGTCACG 
ATCTCACTGG CTTACTCCGT CAAGAAAATG ATGAAGGATA ACAACTTGGT GAGGCACCTG 
GATGCTTGTG AGACCATGGG GAACGCTACA GCTATCTGTT CCGATAAGAC GGGGACCCTG 
ACGATGAACA GGATGACGGT CGTTCAGGCC TACATCAACG AAAAGCATTA TAAAAAGGTT 
CCGGAACCAG AAGCCATCCC GCCAAATATC CTGTCCTATC TGGTAACAGG AATTTCTGTG 
AATTGTGCTT ATACATCAAA AATATTGCCA CCGGAGAAAG AGGGTGGATT ACCTCGTCAT 
GTGGGGAATA AAACAGAATG TGCCTTGTTG GGATTTCTCT TGGATTTAAA GCGGGATTAT 
CAAGATGTCA GAAATGAAAT ACCAGAAGAA GCATTGTACA AAGTCTATAC CTTCAATTCT 
GTTAGGAAAT CCATGAGTAC AGTCCTAAAG AATTCAGATG GAAGTTTCCG GATCTTCAGC 
AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG 
GCCAAAGTGT TCCGACCCAG AGACCGAGAT GACATTGTGA AGACGGTGAT TGAGCCCATG 
GCATCCGAAG GCCTGAGAAC CATATGCCTA GCGTTCAGAG ACTTCCCCGC AGGAGAGCCA 
GAACCAGAGT GGGATAACGA GAACGATGTC GTCACCGGCC TTACTTGCAT CGCTGTTGTG 
GGGATTGAAG ATCCTGTGAG GCCTGAGGTA CCAGAGGCAA TAAAGAAATG TCAGAGGGCT 
GGAATTACCG TGCGCATGGT CACTGGTGAT AATATTAACA CAGCTCGGGC CATTGCTACC 
AAATGCGGTA TTTTACACCC AGGGGAAGAT TTCCTGTGCT TAGAAGGTAA AGATTTTAAC 
CGAAGAATAC GAAATGAAAA AGGAGAGATT GAACAAGAGA GGATAGACAA GATTTGGCCA 
AAGCTTCGAG TGCTTGCAAG ATCATCTCCC ACTGACAAAC ATACACTGGT TAAAGGTATA 
ATTGACAGCA CAGTCTCAGA GCAACGCCAG GTTGTAGCTG TGACAGGGGA TGGTACAAAT 
GACGGGCCTG CGCTGAAGAA AGCAGACGTT GGCTTTGCAA TGGGCATTGC TGGAACTGAT 
GTGGCTAAAG AAGCATCTGA TATCATTCTC ACAGATGACA ACTTCACAAG CATTGTTAAA 
GCGGTTATGT GGGGACGGAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT 
ACTGTTAATG TAGTGGCCGT GATTGTCGCT TTCACGGGCG CTTGTATTAC TCAAGACTCG 
CCACTGAAGG CGGTACAGAT GCTCTGGGTC AACCTCATCA TGGACACGCT GGCCTCCCTG 
GCTCTGGCGA CAGAACCACC CACCGAGTCA CTCCTGCTCC GGAAGCCTTA CGGGAGAAAC 
AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATCTTGG GCCATGCGTT CTATCAGCTC 
GTAGTGGTCT TCACGCTCTT GTTCGCTGGA GAGAAGTTTT TCGATATCGA CAGTGGGAGG 
AACGCCCCTC TGCATGCTCC ACCGTCAGAG CACTACACCA TTGTGTTCAA CACCTTTGTG 
CTGATGCAGC TCTTCAATGA AATAAATGCC CGGAAAATTC ATGGGGAAAG AAATGTGTTT 
GAAGGGATCT TCAACAACGC CATCTTCTGC ACAATTGTCT TAGGCACCTT TGTGGTGCAG 
ATAATAATCG TGCAGTTTGG CGGGAAGCCT TTCAGCTGCT CAGAACTTTC AATAGAACAA 
TGGCTGTGGT CCATATTCCT GGGAATGGGG ACCTTACTCT GGGGCCAGCT TATTTCAACC 
ATTCCAACCA GCCGCTTAAA GTTTCTAAAA GAAGCTGGCC ATGGGACACA GAAGGAAGAG 
ATCCCTGAGG AGGAATTGGC GGAGGATGTT GAAGAGATTG ACCATGCCGA ACGGGAGTTG 
CGGCGTGGCC AGATCTTGTG GTTTAGAGGC CTGAACAGAA TCCAAACACA GATCCGAGTG 
GTGAATGCAT TTCGTAGCTC TTTATACGAA GGGTTAGAGA AGCCAGAATC AAGAAGTTCA 
ATCCACAACT TTATGACACA CCCCGAGTTT AGGATAGAAG ATTCAGAGCC GCACATCCCC 
CTTATCGATG ACACTGATGC TGAAGATGAC GCTCCTACTA AACGCAACTC CAGCCCTCCG 
CCCTCTCCTA ACAAAAATAA CAATGCCGTT GACAGCGGGA TCCACCTTAC AATAGAAATG 
AACAAGTCTG CTACCTCTTC GTCCCCAGGG AGCCCACTGC ATAGTTTGGA AACATCACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A complex of Neuroplastin and Plasma Membrane Ca
Scientific reports7(1)8358(2017 Aug)
Korthals M,Langnaese K,Smalla KH,K?hne T,Herrera-Molina R,Handschuh J,Lehmann AC,Mamula D,Naumann M,Seidenbecher C,Zuschratter W,Tedford K,Gundelfinger ED,Montag D,Fischer KD,Thomas U

ATP2B1 gene Silencing Increases Insulin Sensitivity through Facilitating Akt Activation via the Ca
International journal of biological sciences13(9)1203-1212(2017)
Long Y,Xia JY,Chen SW,Gao CL,Liang GN,He XM,Wu J,Jiang CX,Liu X,Huang W,Wan Q,Xu Y

Deletion of the intestinal plasma membrane calcium pump, isoform 1, Atp2b1, in mice is associated with decreased bone mineral density and impaired responsiveness to 1, 25-dihydroxyvitamin D3.
Biochemical and biophysical research communications467(1)152-6(2015 Nov)
Ryan ZC,Craig TA,Filoteo AG,Westendorf JJ,Cartwright EJ,Neyses L,Strehler EE,Kumar R

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Atp2b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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