Mgat3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mgat3
Entrez Gene ID	29582
Full Name	mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
General protein information	Preferred Names mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase Names beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase GNT-III N-acetylglucosaminyltransferase III N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III glcNAc-T III mannoside acetyl glucosaminyltransferase 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	enzyme that catalyzes the addition of N-acetylglucosamine to the beta-linked mannose of the trimannosyl core of N-linked sugar chains [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594703.1	XP_017450192.1	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
NM_019239.2	NP_062112.2	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
XM_006242073.3	XP_006242135.1	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
XM_006242074.3	XP_006242136.1	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
XM_006242072.2	XP_006242134.1	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mgat3	NP_034925.2
Pan troglodytes (chimpanzee)	MGAT3	XP_525603.2
	MGAT3	XP_001097702.1
Canis lupus familiaris (dog)	MGAT3	XP_005625921.1
Bos taurus (cattle)	MGAT3	XP_005207428.1
Rattus norvegicus (Norway rat)	Mgat3	NP_062112.2
Danio rerio (zebrafish)	LOC570828	XP_699442.5
Xenopus tropicalis (tropical clawed frog)	mgat3	XP_004913814.1
Homo sapiens (human)	MGAT3	NP_002400.3
Gallus gallus (chicken)	MGAT3	NP_001012711.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of N-Acetylglucosaminyltransferase III Suppresses ?2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to ?2,6-Sialylation Levels.
Lu J, Isaji T, Im S, Fukuda T, Kameyama A, Gu J
The Journal of biological chemistry291(11)5708-20(2016 Mar)

Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions.
Kizuka Y, Nakano M, Kitazume S, Saito T, Saido TC, Taniguchi N
The Biochemical journal473(1)21-30(2016 Jan)

An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido TC, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
EMBO molecular medicine7(2)175-89(2015 Feb)

The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function.
Kurimoto A, Kitazume S, Kizuka Y, Nakajima K, Oka R, Fujinawa R, Korekane H, Yamaguchi Y, Wada Y, Taniguchi N
The Journal of biological chemistry289(17)11704-14(2014 Apr)

N-Glycosylation engineering of lepidopteran insect cells by the introduction of the beta1,4-N-acetylglucosaminyltransferase III gene.
Okada T, Ihara H, Ito R, Nakano M, Matsumoto K, Yamaguchi Y, Taniguchi N, Ikeda Y
Glycobiology20(9)1147-59(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GnT-III transfection has the potential to be an effective approach in humanizing the N-glycosylation of lepidopteran insect cells.
Title: N-Glycosylation engineering of lepidopteran insect cells by the introduction of the beta1,4-N-acetylglucosaminyltransferase III gene.

The following Mgat3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mgat3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04123	NM_019239.2 Latest version!	Rattus norvegicus beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (Mgat3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa04123	XM_006242073.3 Latest version!	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa04123	XM_006242072.2 Latest version!	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa04123	XM_017594703.1 Latest version!	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa04123	XM_006242074.3 Latest version!	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mgat3 ( NM_019239.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062112.2 Rattus norvegicus beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (Mgat3), mRNA.

Price & Availability↑

CloneID	ORa04123
Clone ID Related Accession (Same CDS sequence)	XM_017594703.1 , NM_019239.2 , XM_006242073.3 , XM_006242074.3 , XM_006242072.2
Accession Version	NM_019239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127784.4. On Oct 17, 2007 this sequence version replaced NM_019239.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: D10852.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062112.2
CDS	52..1668
Translation	MKMRRYKLFLMFCMAGLCLISFLHFFKTLSYVTFPRELASLSPNLISSFFWNNAPVTPQASPEPGDPDLLRTPLYSHSPLLQPLSPSKATEELHRVDFVLPEDTTEYFVRTKAGGVCFKPGTRMLEKPSPGRTEEKTKVAEGSSVRGPARRPMRHVLSARERLGGRGTRRKWVECVCLPGWHGPSCGVPTVVQYSNLPTKERLVPREVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRSLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDQFRYLLENPYREPKSTVEGGRRNQGSDGRSSAVRGKLDTTEG

Target ORF information:

RefSeq Version	NM_019239.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (Mgat3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019239.2

ORF Insert Sequence:

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mgat3 ( XM_017594703.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450192.1 Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04123
Clone ID Related Accession (Same CDS sequence)	XM_017594703.1 , NM_019239.2 , XM_006242073.3 , XM_006242074.3 , XM_006242072.2
Accession Version	XM_017594703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450192.1
CDS	1600..3216
Translation	MKMRRYKLFLMFCMAGLCLISFLHFFKTLSYVTFPRELASLSPNLISSFFWNNAPVTPQASPEPGDPDLLRTPLYSHSPLLQPLSPSKATEELHRVDFVLPEDTTEYFVRTKAGGVCFKPGTRMLEKPSPGRTEEKTKVAEGSSVRGPARRPMRHVLSARERLGGRGTRRKWVECVCLPGWHGPSCGVPTVVQYSNLPTKERLVPREVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRSLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDQFRYLLENPYREPKSTVEGGRRNQGSDGRSSAVRGKLDTTEG

Target ORF information:

RefSeq Version	XM_017594703.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594703.1

ORF Insert Sequence:

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mgat3 ( XM_006242073.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242135.1 Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa04123
Clone ID Related Accession (Same CDS sequence)	XM_017594703.1 , NM_019239.2 , XM_006242073.3 , XM_006242074.3 , XM_006242072.2
Accession Version	XM_006242073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242135.1
CDS	315..1931
Translation	MKMRRYKLFLMFCMAGLCLISFLHFFKTLSYVTFPRELASLSPNLISSFFWNNAPVTPQASPEPGDPDLLRTPLYSHSPLLQPLSPSKATEELHRVDFVLPEDTTEYFVRTKAGGVCFKPGTRMLEKPSPGRTEEKTKVAEGSSVRGPARRPMRHVLSARERLGGRGTRRKWVECVCLPGWHGPSCGVPTVVQYSNLPTKERLVPREVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRSLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDQFRYLLENPYREPKSTVEGGRRNQGSDGRSSAVRGKLDTTEG

Target ORF information:

RefSeq Version	XM_006242073.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242073.3

ORF Insert Sequence:

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mgat3 ( XM_006242074.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242136.1 Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa04123
Clone ID Related Accession (Same CDS sequence)	XM_017594703.1 , NM_019239.2 , XM_006242073.3 , XM_006242074.3 , XM_006242072.2
Accession Version	XM_006242074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242136.1
CDS	24..1640
Translation	MKMRRYKLFLMFCMAGLCLISFLHFFKTLSYVTFPRELASLSPNLISSFFWNNAPVTPQASPEPGDPDLLRTPLYSHSPLLQPLSPSKATEELHRVDFVLPEDTTEYFVRTKAGGVCFKPGTRMLEKPSPGRTEEKTKVAEGSSVRGPARRPMRHVLSARERLGGRGTRRKWVECVCLPGWHGPSCGVPTVVQYSNLPTKERLVPREVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRSLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDQFRYLLENPYREPKSTVEGGRRNQGSDGRSSAVRGKLDTTEG

Target ORF information:

RefSeq Version	XM_006242074.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242074.3

ORF Insert Sequence:

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mgat3 ( XM_006242072.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242134.1 Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04123
Clone ID Related Accession (Same CDS sequence)	XM_017594703.1 , NM_019239.2 , XM_006242073.3 , XM_006242074.3 , XM_006242072.2
Accession Version	XM_006242072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006242072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242134.1
CDS	147..1763
Translation	MKMRRYKLFLMFCMAGLCLISFLHFFKTLSYVTFPRELASLSPNLISSFFWNNAPVTPQASPEPGDPDLLRTPLYSHSPLLQPLSPSKATEELHRVDFVLPEDTTEYFVRTKAGGVCFKPGTRMLEKPSPGRTEEKTKVAEGSSVRGPARRPMRHVLSARERLGGRGTRRKWVECVCLPGWHGPSCGVPTVVQYSNLPTKERLVPREVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRSLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDQFRYLLENPYREPKSTVEGGRRNQGSDGRSSAVRGKLDTTEG

Target ORF information:

RefSeq Version	XM_006242072.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (Mgat3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242072.2

ORF Insert Sequence:

ATGAAGATGA GACGCTACAA GCTTTTTCTC ATGTTCTGTA TGGCCGGCCT GTGCCTCATC 
TCCTTCCTGC ACTTCTTTAA GACGTTATCC TATGTCACCT TCCCGAGAGA ACTGGCCTCC 
CTCAGCCCTA ACCTCATATC CAGCTTCTTC TGGAACAATG CCCCTGTCAC TCCCCAGGCC 
AGTCCGGAGC CCGGTGACCC CGACTTGTTA CGGACTCCAC TCTACTCCCA CTCCCCCCTG 
CTCCAGCCAC TGTCCCCTAG CAAGGCCACC GAAGAACTGC ACCGGGTGGA CTTCGTGTTG 
CCGGAGGACA CCACAGAGTA TTTTGTGCGC ACCAAAGCTG GCGGTGTGTG CTTCAAACCA 
GGTACCAGGA TGCTGGAGAA ACCTTCTCCA GGGCGGACAG AGGAGAAGAC CAAGGTGGCT 
GAGGGGTCCT CGGTCCGGGG TCCTGCTCGG AGGCCTATGC GGCATGTGTT GAGTGCACGG 
GAGCGCCTGG GAGGCCGGGG CACTAGGCGC AAGTGGGTTG AGTGTGTGTG CCTGCCAGGC 
TGGCACGGGC CCAGCTGCGG GGTGCCCACT GTGGTCCAGT ATTCCAACCT GCCCACCAAG 
GAGCGCCTGG TACCCAGGGA GGTGCCGAGG CGGGTTATCA ACGCCATCAA CATCAACCAT 
GAGTTCGACC TGCTGGATGT GCGCTTCCAT GAGCTGGGCG ATGTTGTGGA CGCCTTTGTG 
GTCTGCGAAT CCAATTTCAC CGCCTACGGG GAGCCTCGGC CGCTCAAGTT CCGAGAGATG 
CTGACCAATG GCACCTTCGA GTACATCCGC CACAAGGTGC TCTACGTCTT CCTGGACCAC 
TTCCCACCTG GTGGCCGTCA GGACGGCTGG ATTGCAGACG ACTACCTGCG TACCTTCCTC 
ACCCAGGATG GTGTCTCCCG CCTGCGCAAC CTGCGACCTG ATGACGTCTT TATCATCGAC 
GACGCGGACG AGATCCCTGC GCGTGATGGT GTGCTGTTCC TCAAGCTCTA CGATGGCTGG 
ACAGAGCCCT TCGCCTTCCA TATGCGCAAG TCCCTGTATG GTTTCTTTTG GAAGCAACCA 
GGCACACTGG AGGTGGTGTC AGGCTGCACC ATTGACATGC TGCAGGCTGT GTATGGGCTG 
GACGGCATCC GCCTGCGCCG CCGTCAGTAC TACACCATGC CCAACTTTCG ACAGTATGAG 
AACCGCACCG GCCACATCCT AGTGCAGTGG TCTCTCGGCA GCCCCCTGCA CTTCGCGGGC 
TGGCACTGCT CCTGGTGCTT CACACCCGAG GGCATCTACT TCAAACTCGT GTCGGCCCAG 
AATGGTGACT TCCCCCGCTG GGGTGACTAC GAGGACAAGA GGGACCTCAA TTACATCCGA 
AGCTTGATTC GCACTGGGGG ATGGTTCGAC GGCACGCAGC AGGAGTACCC TCCTGCAGAC 
CCCAGTGAAC ACATGTATGC TCCTAAGTAC CTGCTCAAGA ACTATGACCA GTTCCGCTAC 
TTGCTCGAAA ATCCCTACCG GGAGCCCAAG AGCACTGTAG AGGGTGGGCG CCGGAACCAG 
GGCTCAGACG GAAGGTCATC TGCTGTCAGG GGCAAGTTGG ATACAACGGA GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of N-Acetylglucosaminyltransferase III Suppresses ?2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to ?2,6-Sialylation Levels.
The Journal of biological chemistry291(11)5708-20(2016 Mar)
Lu J,Isaji T,Im S,Fukuda T,Kameyama A,Gu J

Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions.
The Biochemical journal473(1)21-30(2016 Jan)
Kizuka Y,Nakano M,Kitazume S,Saito T,Saido TC,Taniguchi N

An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
EMBO molecular medicine7(2)175-89(2015 Feb)
Kizuka Y,Kitazume S,Fujinawa R,Saito T,Iwata N,Saido TC,Nakano M,Yamaguchi Y,Hashimoto Y,Staufenbiel M,Hatsuta H,Murayama S,Manya H,Endo T,Taniguchi N

The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function.
The Journal of biological chemistry289(17)11704-14(2014 Apr)
Kurimoto A,Kitazume S,Kizuka Y,Nakajima K,Oka R,Fujinawa R,Korekane H,Yamaguchi Y,Wada Y,Taniguchi N

N-Glycosylation engineering of lepidopteran insect cells by the introduction of the beta1,4-N-acetylglucosaminyltransferase III gene.
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Mgat3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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