Scn10a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scn10a
Entrez Gene ID	29571
Full Name	sodium voltage-gated channel alpha subunit 10
Synonyms	Na(V)1.8,Nav1.8,PN3
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 10 Names sodium channel protein type 10 subunit alpha peripheral nerve sodium channel 3 sensory neuron sodium channel sodium channel protein type X subunit alpha sodium channel type X alpha polypeptide sodium channel voltage-gated type X alpha polypeptide sodium channel, voltage-gated, type 10, alpha polypeptide sodium channel, voltage-gated, type X, alpha subunit voltage-gated sodium channel subunit alpha Nav1.8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017247.1	NP_058943.1	sodium channel protein type 10 subunit alpha
XM_017595503.1	XP_017450992.1	sodium channel protein type 10 subunit alpha isoform X1
XM_017595504.1	XP_017450993.1	sodium channel protein type 10 subunit alpha isoform X1
XM_017595505.1	XP_017450994.1	sodium channel protein type 10 subunit alpha isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN10A	NP_006505.2
Rattus norvegicus (Norway rat)	Scn10a	NP_058943.1
Canis lupus familiaris (dog)	SCN10A	NP_001003203.1
Bos taurus (cattle)	SCN10A	XP_002696962.1
Pan troglodytes (chimpanzee)	SCN10A	XP_516372.3
Mus musculus (house mouse)	Scn10a	NP_001192250.1
	SCN10A	XP_002808374.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of cystathionine ?-synthetase suppresses sodium channel activities of dorsal root ganglion neurons of rats with lumbar disc herniation.
Yan J, Hu S, Zou K, Xu M, Wang Q, Miao X, Yu SP, Xu GY
Scientific reports638188(2016 Dec)

Modulation of Nav1.8 by Lysophosphatidic Acid in the Induction of Bone Cancer Pain.
Pan HL, Liu BL, Lin W, Zhang YQ
Neuroscience bulletin32(5)445-54(2016 Oct)

Changes in the expression of voltage-gated sodium channels Nav1.3, Nav1.7, Nav1.8, and Nav1.9 in rat trigeminal ganglia following chronic constriction injury.
Xu W, Zhang J, Wang Y, Wang L, Wang X
Neuroreport27(12)929-34(2016 Aug)

Functional up-regulation of Nav1.8 sodium channel on dorsal root ganglia neurons contributes to the induction of scorpion sting pain.
Ye P, Hua L, Jiao Y, Li Z, Qin S, Fu J, Jiang F, Liu T, Ji Y
Acta biochimica et biophysica Sinica48(2)132-44(2016 Feb)

SDF1-CXCR4 signaling contributes to persistent pain and hypersensitivity via regulating excitability of primary nociceptive neurons: involvement of ERK-dependent Nav1.8 up-regulation.
Yang F, Sun W, Yang Y, Wang Y, Li CL, Fu H, Wang XL, Yang F, He T, Chen J
Journal of neuroinflammation12219(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated the modulation of Nav1.8 by LPA in DRG neurons, and that this probably underlies the peripheral mechanism by which bone cancer pain is induced.
Title: Modulation of Nav1.8 by Lysophosphatidic Acid in the Induction of Bone Cancer Pain.

Study found that trigeminal ganglion in rats subjected to an infraorbital nerve-chronic constriction injury showed upregulation of Nav1.3 and downregulation of Nav1.7, Nav1.8, and Nav1.9 messenger RNA and protein levels. Findings suggest that voltage-gated sodium channels may participate in the regulation of trigeminal neuralgia.
Title: Changes in the expression of voltage-gated sodium channels Nav1.3, Nav1.7, Nav1.8, and Nav1.9 in rat trigeminal ganglia following chronic constriction injury.

investigated whether the aberrant expression of Nav1.8 in dorsal root ganglia contributes to generation of pain induced by scorpion stings
Title: Functional up-regulation of Nav1.8 sodium channel on dorsal root ganglia neurons contributes to the induction of scorpion sting pain.

Up-regulation of Nav1.8 in nociceptive neurons contributes to persistent pain regulated by SDF1-CXCR4 signaling.
Title: SDF1-CXCR4 signaling contributes to persistent pain and hypersensitivity via regulating excitability of primary nociceptive neurons: involvement of ERK-dependent Nav1.8 up-regulation.

The study demonstrates that positive shift of Nav1.8 current inactivation curve in injured neurons causes neuropathic pain following chronic constriction injury.
Title: Positive shift of Nav1.8 current inactivation curve in injured neurons causes neuropathic pain following chronic constriction injury.

The following Scn10a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scn10a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03383	NM_017247.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$1700.30 $2429.00
ORa62343	XM_017595503.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa62343	XM_017595505.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa62343	XM_017595504.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scn10a ( NM_017247.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058943.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), mRNA.

Price & Availability↑

CloneID	ORa03383
Clone ID Related Accession (Same CDS sequence)	NM_017247.1
Accession Version	NM_017247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 10 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X92184.1. On Sep 6, 2004 this sequence version replaced XM_346840.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X92184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04563757, SAMN04563759 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGACTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGAAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACC 
CCTGATGATG GGGTCTTTCA CGGAGACCAG GAAAGCCGTC GAGGTTCCAT ATTGCTGGGC 
AGGGGTGCTG GGCAGACAGG TCCACTCCCC AGGAGCCCAC TGCCTCAGTC CCCCAACCCT 
GGCCGTAGAC ATGGAGAAGA GGGACAGCTC GGAGTGCCCA CTGGTGAGCT TACCGCTGGA 
GCGCCTGAAG GCCCGGCACT GCACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGTGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTACGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCAG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCACTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGGGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCCTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTAGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTACAT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG CCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058943.1
CDS	204..6077
Translation	MELPFASVGTTNFRRFTPESLAEIEKQIAAHRAAKKARTKHRGQEDKGEKPRPQLDLKDCNQLPKFYGELPAELVGEPLEDLDPFYSTHRTFMVLNKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITITILVNCVCMTRTDLPEKVEYVFTVIYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRGISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIRNGTDPHKADNLSSEMAEYIFIKPGTTDPLLCGNGSDAGHCPGGYVCLKTPDNPDFNYTSFDSFAWAFLSLFRLMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFQEALEVLQKEQEVLEALGIDTTSLQSHSGSPLASKNANERRPRVKSRVSEGSTDDNRSPQSDPYNQRRMSFLGLSSGRRRASHGSVFHFRAPSQDISFPDGITPDDGVFHGDQESRRGSILLGRGAGQTGPLPRSPLPQSPNPGRRHGEEGQLGVPTGELTAGAPEGPALHTTGQKSFLSAGYLNEPFRAQRAMSVVSIMTSVIEELEESKLKCPPCLISFAQKYLIWECCPKWRKFKMALFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGNIVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSASKKGSLSVLRTLRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSEDYGCRKDGVSVWNGEKLRWHMCDFFHSFLVVFRILCGEWIENMWVCMEVSQKSICLILFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQLALARIQVLGHRASRAIASYISSHCRFHWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALSSPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSPASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEGCTRRCPCCNVNTSKSPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYTDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLRALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSKCVDTRNNPFSNVNSTMVNNKSECHNQNSTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSGEINSQPNWENNLYMYLYFVVFIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFVFDIVTRQAFDIIIMVLICLNMITMMVETDEQGEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGWNVFDFIVVILSIGSLLFSAILKSLENYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMASFANVVDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGIIFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWEKFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELDSLKTNMEEKFMATNLSKASYEPIATTLRWKQEDLSATVIQKAYRSYMLHRSLTLSNTLHVPRAEEDGVSLPGEGYITFMANSGLPDKSETASATSFPPSYDSVTRGLSDRANINPSSSMQNEDEVAAKEGNSPGPQ

Target ORF information:

RefSeq Version	NM_017247.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017247.1

ORF Insert Sequence:

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGACTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGAAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACC 
CCTGATGATG GGGTCTTTCA CGGAGACCAG GAAAGCCGTC GAGGTTCCAT ATTGCTGGGC 
AGGGGTGCTG GGCAGACAGG TCCACTCCCC AGGAGCCCAC TGCCTCAGTC CCCCAACCCT 
GGCCGTAGAC ATGGAGAAGA GGGACAGCTC GGAGTGCCCA CTGGTGAGCT TACCGCTGGA 
GCGCCTGAAG GCCCGGCACT GCACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGTGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTACGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCAG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCACTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGGGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCCTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTAGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTACAT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG CCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn10a ( XM_017595503.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450992.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X1, mRNA.

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CloneID	ORa62343
Clone ID Related Accession (Same CDS sequence)	XM_017595503.1 , XM_017595504.1 , XM_017595505.1
Accession Version	XM_017595503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 10 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450992.1
CDS	254..6127
Translation	MELPFASVGTTNFRRFTPESLAEIEKQIAAHRAAKKARTKHRGQEDKGEKPRPQLDLKACNQLPKFYGELPAELVGEPLEDLDPFYSTHRTFMVLNKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITITILVNCVCMTRTDLPEKVEYVFTVIYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRGISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIRNGTDPHKADNLSSEMAEYIFIKPGTTDPLLCGNGSDAGHCPGGYVCLKTPDNPDFNYTSFDSFAWAFLSLFRLMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFQEALEVLQKEQEVLAALGIDTTSLQSHSGSPLASKNANERRPRVKSRVSEGSTDDNRSPQSDPYNQRRMSFLGLSSGRRRASHGSVFHFRAPSQDISFPDGITDDGVFHGDQESRRGSILLGRGAGQTGPLPRSPLPQSPNPGRRHGEEGQLGVPTGELTAGAPEGPQALDTTGQKSFLSAGYLNEPFRAQRAMSVVSIMTSVIEELEESKLKCPPCLISFAQKYLIWECCPKWRKFKMALFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGNIVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSASKKGSLSVLRTFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSEDYGCRKDGVSVWNGEKLRWHMCDFFHSFLVVFRILCGEWIENMWVCMEVSQKSICLILFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQLALARIQVLGHRASRAIASYISSHCRFRWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALSSPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSPASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEGCTRRCPCCNVNTSKSPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYTDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLRALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSKCVDTRNNPFSNVNSTMVNNKSECHNQNSTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSGEINSQPNWENNLYMYLYFVVFIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFVFDIVTRQAFDIIIMVLICLNMITMMVETDEQGEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGWNVFDFIVVILSIGSLLFSAILKSLENYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMASFANVVDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGIIFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWEKFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELDSLKTNMEEKFMATNLSKASYEPIATTLRWKQEDLSATVIQKAYRSYMLHRSLTLSNTLHVPRAEEDGVSLPGEGYVTFMANSGLPDKSETASATSFPPSYDSVTRGLSDRANINPSSSMQNEDEVAAKEGNSPGPQ

Target ORF information:

RefSeq Version	XM_017595503.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595503.1

ORF Insert Sequence:

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn10a ( XM_017595504.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450993.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X2, mRNA.

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CloneID	ORa62343
Clone ID Related Accession (Same CDS sequence)	XM_017595503.1 , XM_017595504.1 , XM_017595505.1
Accession Version	XM_017595504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 10 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450993.1
CDS	223..6096
Translation	MELPFASVGTTNFRRFTPESLAEIEKQIAAHRAAKKARTKHRGQEDKGEKPRPQLDLKACNQLPKFYGELPAELVGEPLEDLDPFYSTHRTFMVLNKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITITILVNCVCMTRTDLPEKVEYVFTVIYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRGISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIRNGTDPHKADNLSSEMAEYIFIKPGTTDPLLCGNGSDAGHCPGGYVCLKTPDNPDFNYTSFDSFAWAFLSLFRLMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFQEALEVLQKEQEVLAALGIDTTSLQSHSGSPLASKNANERRPRVKSRVSEGSTDDNRSPQSDPYNQRRMSFLGLSSGRRRASHGSVFHFRAPSQDISFPDGITDDGVFHGDQESRRGSILLGRGAGQTGPLPRSPLPQSPNPGRRHGEEGQLGVPTGELTAGAPEGPQALDTTGQKSFLSAGYLNEPFRAQRAMSVVSIMTSVIEELEESKLKCPPCLISFAQKYLIWECCPKWRKFKMALFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGNIVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSASKKGSLSVLRTFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSEDYGCRKDGVSVWNGEKLRWHMCDFFHSFLVVFRILCGEWIENMWVCMEVSQKSICLILFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQLALARIQVLGHRASRAIASYISSHCRFRWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALSSPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSPASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEGCTRRCPCCNVNTSKSPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYTDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLRALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSKCVDTRNNPFSNVNSTMVNNKSECHNQNSTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSGEINSQPNWENNLYMYLYFVVFIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFVFDIVTRQAFDIIIMVLICLNMITMMVETDEQGEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGWNVFDFIVVILSIGSLLFSAILKSLENYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMASFANVVDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGIIFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWEKFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELDSLKTNMEEKFMATNLSKASYEPIATTLRWKQEDLSATVIQKAYRSYMLHRSLTLSNTLHVPRAEEDGVSLPGEGYVTFMANSGLPDKSETASATSFPPSYDSVTRGLSDRANINPSSSMQNEDEVAAKEGNSPGPQ

Target ORF information:

RefSeq Version	XM_017595504.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595504.1

ORF Insert Sequence:

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn10a ( XM_017595505.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450994.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X3, mRNA.

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CloneID	ORa62343
Clone ID Related Accession (Same CDS sequence)	XM_017595503.1 , XM_017595504.1 , XM_017595505.1
Accession Version	XM_017595505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 10 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450994.1
CDS	410..6283
Translation	MELPFASVGTTNFRRFTPESLAEIEKQIAAHRAAKKARTKHRGQEDKGEKPRPQLDLKACNQLPKFYGELPAELVGEPLEDLDPFYSTHRTFMVLNKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITITILVNCVCMTRTDLPEKVEYVFTVIYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRGISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIRNGTDPHKADNLSSEMAEYIFIKPGTTDPLLCGNGSDAGHCPGGYVCLKTPDNPDFNYTSFDSFAWAFLSLFRLMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFQEALEVLQKEQEVLAALGIDTTSLQSHSGSPLASKNANERRPRVKSRVSEGSTDDNRSPQSDPYNQRRMSFLGLSSGRRRASHGSVFHFRAPSQDISFPDGITDDGVFHGDQESRRGSILLGRGAGQTGPLPRSPLPQSPNPGRRHGEEGQLGVPTGELTAGAPEGPQALDTTGQKSFLSAGYLNEPFRAQRAMSVVSIMTSVIEELEESKLKCPPCLISFAQKYLIWECCPKWRKFKMALFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGNIVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSASKKGSLSVLRTFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSEDYGCRKDGVSVWNGEKLRWHMCDFFHSFLVVFRILCGEWIENMWVCMEVSQKSICLILFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQLALARIQVLGHRASRAIASYISSHCRFRWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALSSPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSPASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEGCTRRCPCCNVNTSKSPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYTDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLRALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSKCVDTRNNPFSNVNSTMVNNKSECHNQNSTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSGEINSQPNWENNLYMYLYFVVFIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFVFDIVTRQAFDIIIMVLICLNMITMMVETDEQGEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGWNVFDFIVVILSIGSLLFSAILKSLENYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMASFANVVDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGIIFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWEKFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELDSLKTNMEEKFMATNLSKASYEPIATTLRWKQEDLSATVIQKAYRSYMLHRSLTLSNTLHVPRAEEDGVSLPGEGYVTFMANSGLPDKSETASATSFPPSYDSVTRGLSDRANINPSSSMQNEDEVAAKEGNSPGPQ

Target ORF information:

RefSeq Version	XM_017595505.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 10 (Scn10a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595505.1

ORF Insert Sequence:

ATGGAGCTCC CCTTTGCGTC CGTGGGAACT ACCAATTTCA GACGGTTCAC TCCAGAGTCA 
CTGGCAGAGA TCGAGAAGCA GATTGCTGCT CACCGCGCAG CCAAGAAGGC CAGAACCAAG 
CACAGAGGAC AGGAGGACAA GGGCGAGAAG CCCAGGCCTC AGCTGGACTT GAAAGCCTGT 
AACCAGCTGC CCAAGTTCTA TGGTGAGCTC CCAGCAGAAC TGGTCGGGGA GCCCCTGGAG 
GACCTAGACC CTTTCTACAG CACACACCGG ACATTCATGG TGTTGAATAA AAGCAGGACC 
ATTTCCAGAT TCAGTGCCAC TTGGGCCCTG TGGCTCTTCA GTCCCTTCAA CCTGATCAGA 
AGAACAGCCA TCAAAGTGTC TGTCCATTCC TGGTTCTCCA TATTCATCAC CATCACTATT 
TTGGTCAACT GCGTGTGCAT GACCCGAACT GATCTTCCAG AGAAAGTCGA GTACGTCTTC 
ACTGTCATTT ACACCTTCGA GGCTCTGATT AAGATACTGG CAAGAGGGTT TTGTCTAAAT 
GAGTTCACTT ATCTTCGAGA TCCGTGGAAC TGGCTGGACT TCAGTGTCAT TACCTTGGCG 
TATGTGGGTG CAGCGATAGA CCTCCGAGGA ATCTCAGGCC TGCGGACATT CCGAGTTCTC 
AGAGCCCTGA AAACTGTTTC TGTGATCCCA GGACTGAAGG TCATCGTGGG AGCCCTGATC 
CACTCAGTGA GGAAGCTGGC CGACGTGACT ATCCTCACAG TCTTCTGCCT GAGCGTCTTC 
GCCTTGGTGG GCCTGCAGCT CTTTAAGGGG AACCTTAAGA ACAAATGCAT CAGGAACGGA 
ACAGATCCCC ACAAGGCTGA CAACCTCTCA TCTGAAATGG CAGAATACAT CTTCATCAAG 
CCTGGTACTA CGGATCCCTT ACTGTGCGGC AATGGGTCTG ATGCTGGTCA CTGCCCTGGA 
GGCTATGTCT GCCTGAAAAC TCCTGACAAC CCGGATTTTA ACTACACCAG CTTTGATTCC 
TTTGCGTGGG CATTCCTCTC ACTGTTCCGC CTCATGACGC AGGACTCCTG GGAGCGCCTG 
TACCAGCAGA CACTCCGGGC TTCTGGGAAA ATGTACATGG TCTTTTTCGT GCTGGTTATT 
TTCCTTGGAT CGTTCTACCT GGTCAATTTG ATCTTGGCCG TGGTCACCAT GGCGTATGAA 
GAGCAGAGCC AGGCAACAAT TGCAGAAATC GAAGCCAAGG AAAAAAAGTT CCAGGAAGCC 
CTTGAGGTGC TGCAGAAGGA ACAGGAGGTG CTGGCAGCCC TGGGGATTGA CACGACCTCG 
CTCCAGTCCC ACAGTGGATC ACCCTTAGCC TCCAAAAACG CCAATGAGAG AAGACCCAGG 
GTGAAATCAA GGGTGTCAGA GGGCTCCACG GATGACAACA GGTCACCCCA ATCTGACCCT 
TACAACCAGC GCAGGATGTC TTTCCTAGGC CTGTCTTCAG GAAGACGCAG GGCTAGCCAC 
GGCAGTGTGT TCCACTTCCG AGCGCCCAGC CAAGACATCT CATTTCCTGA CGGGATCACG 
GATGATGGGG TCTTTCACGG AGACCAGGAA AGCCGTCGAG GTTCCATATT GCTGGGCAGG 
GGTGCTGGGC AGACAGGTCC ACTCCCCAGG AGCCCACTGC CTCAGTCCCC CAACCCTGGC 
CGTAGACATG GAGAAGAGGG ACAGCTCGGA GTGCCCACTG GTGAGCTTAC CGCTGGAGCG 
CCTGAAGGCC CGCAGGCACT CGACACTACA GGGCAGAAGA GCTTCCTGTC TGCGGGCTAC 
TTGAACGAAC CTTTCCGAGC ACAGAGGGCC ATGAGCGTTG TCAGTATCAT GACTTCTGTC 
ATTGAGGAGC TTGAAGAGTC TAAGCTGAAG TGCCCACCCT GCTTGATCAG CTTCGCTCAG 
AAGTATCTGA TCTGGGAGTG CTGCCCCAAG TGGAGGAAGT TCAAGATGGC GCTGTTCGAG 
CTGGTGACTG ACCCCTTCGC AGAGCTTACC ATCACCCTCT GCATCGTGGT GAACACCGTC 
TTCATGGCCA TGGAGCACTA CCCCATGACC GATGCCTTCG ATGCCATGCT TCAAGCCGGC 
AACATTGTCT TCACCGTGTT TTTCACAATG GAGATGGCCT TCAAGATCAT TGCCTTCGAC 
CCCTACTATT ACTTCCAGAA GAAGTGGAAT ATCTTCGACT GTGTCATCGT CACCGTGAGC 
CTTCTGGAGC TGAGCGCATC CAAGAAGGGC AGCCTGTCTG TGCTCCGTAC CTTCCGCTTG 
CTGCGGGTCT TCAAGCTGGC CAAGTCCTGG CCCACCCTGA ACACCCTCAT CAAGATCATC 
GGGAACTCCG TGGGGGCCCT GGGCAACCTG ACCTTTATCC TGGCCATCAT CGTCTTCATC 
TTCGCCCTGG TCGGAAAGCA GCTTCTCTCA GAGGACTACG GGTGCCGCAA GGACGGCGTC 
TCCGTGTGGA ACGGCGAGAA GCTCCGCTGG CACATGTGTG ACTTCTTCCA TTCCTTCCTG 
GTCGTCTTCC GAATCCTCTG CGGGGAGTGG ATCGAGAACA TGTGGGTCTG CATGGAGGTC 
AGCCAGAAAT CCATCTGCCT CATCCTCTTC TTGACTGTGA TGGTGCTGGG CAACCTAGTG 
GTGCTCAACC TTTTCATCGC TTTACTGCTG AACTCCTTCA GCGCGGACAA CCTCACGGCT 
CCAGAGGATG ACGGGGAGGT GAACAACTTG CAGTTAGCAC TGGCCAGGAT CCAGGTACTT 
GGCCATCGGG CCAGCAGGGC CATCGCCAGT TACATCAGCA GCCACTGCCG ATTCCGCTGG 
CCCAAGGTGG AGACCCAGCT GGGCATGAAG CCCCCACTCA CCAGCTCAGA GGCCAAGAAC 
CACATTGCCA CTGATGCTGT CAGTGCTGCA GTGGGGAACC TGACAAAGCC AGCTCTCAGT 
AGCCCCAAGG AGAACCACGG GGACTTCATC ACTGATCCCA ACGTGTGGGT CTCTGTGCCC 
ATTGCTGAGG GGGAATCTGA CCTCGACGAG CTCGAGGAAG ATATGGAGCA GGCTTCGCAG 
AGCTCCTGGC AGGAAGAGGA CCCCAAGGGA CAGCAGGAGC AGTTGCCACA AGTCCAAAAG 
TGTGAAAACC ACCAGGCAGC CAGAAGCCCA GCCTCCATGA TGTCCTCTGA GGACCTGGCT 
CCATACCTGG GTGAGAGCTG GAAGAGGAAG GATAGCCCTC AGGTCCCTGC CGAGGGAGTG 
GATGACACGA GCTCCTCTGA GGGCAGCACG GTGGACTGCC CGGACCCAGA GGAAATCCTG 
AGGAAGATCC CCGAGCTGGC AGATGACCTG GACGAGCCCG ATGACTGTTT CACAGAAGGC 
TGCACTCGCC GCTGTCCCTG CTGCAACGTG AATACTAGCA AGTCTCCTTG GGCCACAGGC 
TGGCAGGTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTTGA GAGTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTTG AGGATAACTA CCTGGAAGAG 
AAACCCCGAG TGAAGTCCGT GCTGGAGTAC ACTGACCGAG TGTTCACCTT CATCTTCGTC 
TTTGAGATGC TGCTCAAGTG GGTAGCCTAT GGCTTCAAAA AGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ACTTCCTCAT TGTGAACATC TCCCTGACAA GCCTCATAGC GAAGATCCTT 
GAGTATTCCG ACGTGGCGTC CATCAAAGCC CTTCGGACTC TCCGTGCCCT CCGACCGCTG 
CGGGCTCTGT CTCGATTCGA AGGCATGAGG GTAGTGGTGG ATGCCCTCGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAATTTTCG AAGTGCGTCG ACACCAGAAA TAACCCATTT 
TCCAACGTGA ATTCGACGAT GGTGAATAAC AAGTCCGAGT GTCACAATCA AAACAGCACC 
GGCCACTTCT TCTGGGTCAA CGTCAAAGTC AACTTCGACA ACGTCGCTAT GGGCTACCTC 
GCACTTCTTC AGGTGGCAAC CTTCAAAGGC TGGATGGACA TAATGTATGC AGCTGTTGAT 
TCCGGAGAGA TCAACAGTCA GCCTAACTGG GAGAACAACT TGTACATGTA CCTGTACTTC 
GTCGTTTTCA TCATTTTCGG TGGCTTCTTC ACGCTGAATC TCTTTGTTGG GGTCATAATC 
GACAACTTCA ACCAACAGAA AAAAAAGCTA GGAGGCCAGG ACATCTTCAT GACAGAAGAG 
CAGAAGAAGT ACTACAATGC CATGAAGAAG CTGGGCTCCA AGAAACCCCA GAAGCCCATC 
CCACGGCCCC TGAATAAGTA CCAAGGCTTC GTGTTTGACA TCGTGACCAG GCAAGCCTTT 
GACATCATCA TCATGGTTCT CATCTGCCTC AACATGATCA CCATGATGGT GGAGACCGAC 
GAGCAGGGCG AGGAGAAGAC GAAGGTTCTG GGCAGAATCA ACCAGTTCTT TGTGGCCGTC 
TTCACGGGCG AGTGTGTGAT GAAGATGTTC GCCCTGCGAC AGTACTACTT CACCAACGGC 
TGGAACGTGT TCGACTTCAT AGTGGTGATC CTGTCCATTG GGAGTCTGCT GTTTTCTGCA 
ATCCTTAAGT CACTGGAAAA CTACTTCTCC CCGACGCTCT TCCGAGTCAT CCGTCTGGCC 
AGGATCGGCC GCATCCTCAG GCTGATCCGA GCAGCCAAGG GGATTCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCCCTCTTC AACATCGGCC TCCTCCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAGC TTCGCTAACG TCGTGGACGA GGCCGGCATC 
GACGACATGT TCAACTTCAA GACCTTTGGC AACAGCATGC TGTGCCTGTT CCAGATCACC 
ACCTCGGCCG GCTGGGACGG CCTCCTCAGC CCCATCCTCA ACACGGGGCC TCCCTACTGC 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGGAACT GCGGGAGCCC GGCGGTGGGC 
ATCATCTTCT TCACCACCTA CATCATCATC TCTTTCCTCA TCGTGGTCAA CATGTACATC 
GCAGTGATTC TGGAGAACTT CAACGTGGCC ACCGAGGAGA GCACGGAGCC CCTGAGCGAG 
GACGACTTCG ACATGTTCTA TGAGACCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATTGCCTTTT CTGCCCTCTC AGACTTCGCG GACACGCTCT CCGGCCCTCT TAGAATCCCC 
AAACCCAACC AGAATATATT AATCCAGATG GACCTGCCGT TGGTCCCCGG GGATAAGATC 
CACTGTCTGG ACATCCTTTT TGCCTTCACA AAGAACGTCT TGGGAGAATC CGGGGAGTTG 
GACTCCCTGA AGACCAATAT GGAAGAGAAG TTTATGGCGA CCAATCTCTC CAAAGCATCC 
TATGAACCAA TAGCCACCAC CCTCCGGTGG AAGCAGGAAG ACCTCTCAGC CACAGTCATT 
CAAAAGGCCT ACCGGAGCTA CATGCTGCAC CGCTCCTTGA CACTCTCCAA CACCCTGCAT 
GTGCCCAGGG CTGAGGAGGA TGGCGTGTCA CTTCCCGGGG AAGGCTATGT TACATTCATG 
GCAAACAGTG GACTCCCGGA CAAATCAGAA ACTGCCTCTG CTACGTCTTT CCCGCCATCC 
TATGACAGTG TCACCAGGGG TCTGAGTGAC CGGGCCAACA TTAACCCATC TAGCTCAATG 
CAAAATGAAG ATGAGGTCGC TGCTAAGGAA GGAAACAGCC CTGGACCTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of cystathionine ?-synthetase suppresses sodium channel activities of dorsal root ganglion neurons of rats with lumbar disc herniation.
Scientific reports638188(2016 Dec)
Yan J,Hu S,Zou K,Xu M,Wang Q,Miao X,Yu SP,Xu GY

Modulation of Nav1.8 by Lysophosphatidic Acid in the Induction of Bone Cancer Pain.
Neuroscience bulletin32(5)445-54(2016 Oct)
Pan HL,Liu BL,Lin W,Zhang YQ

Changes in the expression of voltage-gated sodium channels Nav1.3, Nav1.7, Nav1.8, and Nav1.9 in rat trigeminal ganglia following chronic constriction injury.
Neuroreport27(12)929-34(2016 Aug)
Xu W,Zhang J,Wang Y,Wang L,Wang X

Functional up-regulation of Nav1.8 sodium channel on dorsal root ganglia neurons contributes to the induction of scorpion sting pain.
Acta biochimica et biophysica Sinica48(2)132-44(2016 Feb)
Ye P,Hua L,Jiao Y,Li Z,Qin S,Fu J,Jiang F,Liu T,Ji Y

SDF1-CXCR4 signaling contributes to persistent pain and hypersensitivity via regulating excitability of primary nociceptive neurons: involvement of ERK-dependent Nav1.8 up-regulation.
Journal of neuroinflammation12219(2015 Nov)
Yang F,Sun W,Yang Y,Wang Y,Li CL,Fu H,Wang XL,Yang F,He T,Chen J

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Scn10a cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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