Limk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Limk2
Entrez Gene ID	29524
Full Name	LIM domain kinase 2
Synonyms	Limk2b,Link2
General protein information	Preferred Names LIM domain kinase 2 Names LIM domain kinase 2 LIM motif-containing protein kinase 2 LIMK-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	kinase with a LIM-kinase (LIMK) domain; human homolog phosphorylates cofilin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024135.2	NP_077049.2	LIM domain kinase 2
XM_008770279.2	XP_008768501.1	LIM domain kinase 2 isoform X6
XM_008770276.2	XP_008768498.1	LIM domain kinase 2 isoform X2
XM_006251236.3	XP_006251298.1	LIM domain kinase 2 isoform X5
XM_008770278.2	XP_008768500.1	LIM domain kinase 2 isoform X3
XM_008770277.2	XP_008768499.1	LIM domain kinase 2 isoform X4
XM_008770275.2	XP_008768497.1	LIM domain kinase 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Limk2	NP_034848.1
Pan troglodytes (chimpanzee)	LIMK2	XP_003317251.1
Canis lupus familiaris (dog)	LIMK2	XP_852696.1
Rattus norvegicus (Norway rat)	Limk2	NP_077049.2
Bos taurus (cattle)	LIMK2	NP_001033187.1
Gallus gallus (chicken)	LIMK2	NP_990446.1
Xenopus tropicalis (tropical clawed frog)	limk2	NP_001135686.1
Danio rerio (zebrafish)	limk2	NP_001002651.1
Homo sapiens (human)	LIMK2	NP_005560.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan MG, Dionne L, Yong Karst S, Coombs H, Bronson RT, Reinholdt LG, Bergstrom DE, Donahue LR, Cox TC, Murray SA
Developmental biology415(2)216-227(2016 07)

Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.
Ko AR, Hyun HW, Min SJ, Kim JE, Kang TC
Molecular brain858(2015 Oct)

LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission.
Kim JE, Ryu HJ, Kim MJ, Kang TC
Cell death and differentiation21(7)1036-49(2014 Jul)

LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.
Li Y, Hu F, Chen HJ, Du YJ, Xie ZY, Zhang Y, Wang J, Wang Y
Science signaling7(331)ra61(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ET-1 may be one of the upstream effectors for programmed neuronal necrosis through abnormal LIMK2 over-expression by ROCK1.
Title: Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.

Inhibition of LIMK2 expression and rescue of DRP1 function attenuates programmed necrotic neuronal death induced by status epilepticus.
Title: LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission.

Data indicate that LIM motif-containing protein kinases LIMK1 and LIMK2-dependent acin rearrangement is involved in the development of complete Freund's adjuvant (CFA)-induced inflammatory pain.
Title: LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.

these results indicate that LIMK2d is a third LIMK2 isoform which regulates neurite extension and highlights the possible existence of a kinase independent function of LIMK2.
Title: LIMK2d, a truncated isoform of Lim kinase 2 regulates neurite growth in absence of the LIM kinase domain.

The following Limk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Limk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa36264	XM_006251236.3 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa06798	NM_024135.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$377.30 $539.00
ORa43299	XM_008770275.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43300	XM_008770276.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43301	XM_008770277.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43302	XM_008770278.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43303	XM_008770279.2 Latest version!	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Limk2 ( XM_006251236.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251298.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa36264
Clone ID Related Accession (Same CDS sequence)	XM_006251236.3
Accession Version	XM_006251236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTT ACTTGTCTGT CCCGGCTTAC TTCACCTCCA GAGACCCCTT TAGGTGTTCC 
GAGTGCCAGG AGTCCCTCAC CAACTGGTAC TATGAGAAGG ACGGGAAGCT CTACTGCCAC 
AAGGACTACT GGGCCAAGTT TGGAGAATTC TGCCACGGGT GCTCCCTGCT GATGACAGGG 
CCAGCCATGG TGGCTGGGGA GTTTAAGTAC CACCCAGAGT GCTTTGCCTG CATGAGTTGC 
AAGGTGATCA TCGAGGATGG GGATGCGTAC GCCCTGGTGC AGCATGCTAC ACTCTACTGC 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AAAGACTGTC TACGGAGTCT 
GTCCAGGACC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCTGCCAC CACCGAGTGC 
AGACGTGGCT TCTCTGTGTC TGTGGAAAGC GCCTCCTCTA ACTATGCCAC CACCGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGC CCCAACAACC GAAATGCCAT CCACCCCGGG 
GACCGCATCT TGGAGATCAA TGGGACCCCT GTCCGCACTC TCCGAGTAGA AGAGGTGGAG 
GATGCGATTA ACCAGACGAG CCAGACACTT CAACTGCTGA TTGAACATGA CCCTGTCCCC 
CAGCGCCTGG ACCAGTTACG GCTAGACACC CGGCTTTCTC CCCACATGCA GAGTAGTGGA 
CATACTCTCA TGCTCAGCAC CTTGGACGCC AAGGAAAATC AGGAGGGAAC ACTGAGGAGA 
CGTTCTCTGA GGCGCAGTAA CAGCATCTCC AAGTCTCCTG GCCCCAGCTC CCCCAAGGAG 
CCGCTGCTCC TGAGCCGAGA CATCAGTCGC TCAGAGTCCC TTCGCTGCTC CAGCAGCTAC 
TCACAGCAGA TCTTCCGGCC CTGTGACCTG ATCCACGGGG AGGTCCTGGG GAAGGGCTTC 
TTCGGACAGG CCATCAAGGT GACTCACAAA GCCACAGGCA AAGTGATGGT CATGAAGGAG 
TTAATTCGCT GTGATGAGGA AACCCAGAAG ACTTTCCTGA CTGAGGTGAA AGTGATGCGA 
AGCCTGGACC ACCCTAACGT GCTTAAGTTC ATCGGGGTCC TGTACAAGGA CAAGAAGCTG 
AATCTGCTGA CAGAGTACAT TGAGGGGGGC ACCCTGAAGG ACTTTCTGCG CAATGTGGAC 
CCGTTTCCCT GGCAACAGAA GGTCAGGTTT GCCAAAGGCA TCGCTTCCGG AATGGCTTAT 
TTACACTCCA TGTGCATCAT CCACCGAGAT CTGAACTCGC ACAACTGTCT CATCAAATTG 
GACAAGACGG TGGTGGTAGC AGACTTCGGG CTGTCACGTC TCATAGTGGA AGAGAGGAAA 
AGGCCTCCAG TAGAGAAGGC CGCCACCAAG AAACGCACCT TACGCAAGAG TGATCGCAAG 
AAGCGCTACA CTGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAATGGCAAG 
AGCTACGATG AGACTGTGGA TGTCTTCTCT TTTGGGATTG TTCTCTGTGA GATCATTGGG 
CAGGTATATG CTGATCCCGA CTGCCTTCCC CGCACACTGG ACTTCGGCCT CAACGTAAAG 
CTTTTCTGGG AGAAGTTTGT CCCAACAGAC TGTCCCCCAG CCTTCTTCCC CCTGGCTGCT 
ATCTGCTGCA AACTGGAGCC TGAGAGCAGA CCGGCATTCT CAAAGCTGGA GGACTCATTC 
GAGGCGCTCT CCCTGTTCTT GGGAGAGTTG GCCATCCCAC TGCCAGCAGA GCTGGAAGAG 
CTGGACCACA CTGTGAGCAT GGAGTATGGC CTAACCCGGG ACTCGCCACC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251298.1
CDS	1448..3301
Translation	MGSYLSVPAYFTSRDPFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEELDHTVSMEYGLTRDSPP

Target ORF information:

RefSeq Version	XM_006251236.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251236.3

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCTGT CCCGGCTTAC TTCACCTCCA GAGACCCCTT TAGGTGTTCC 
GAGTGCCAGG AGTCCCTCAC CAACTGGTAC TATGAGAAGG ACGGGAAGCT CTACTGCCAC 
AAGGACTACT GGGCCAAGTT TGGAGAATTC TGCCACGGGT GCTCCCTGCT GATGACAGGG 
CCAGCCATGG TGGCTGGGGA GTTTAAGTAC CACCCAGAGT GCTTTGCCTG CATGAGTTGC 
AAGGTGATCA TCGAGGATGG GGATGCGTAC GCCCTGGTGC AGCATGCTAC ACTCTACTGC 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AAAGACTGTC TACGGAGTCT 
GTCCAGGACC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCTGCCAC CACCGAGTGC 
AGACGTGGCT TCTCTGTGTC TGTGGAAAGC GCCTCCTCTA ACTATGCCAC CACCGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGC CCCAACAACC GAAATGCCAT CCACCCCGGG 
GACCGCATCT TGGAGATCAA TGGGACCCCT GTCCGCACTC TCCGAGTAGA AGAGGTGGAG 
GATGCGATTA ACCAGACGAG CCAGACACTT CAACTGCTGA TTGAACATGA CCCTGTCCCC 
CAGCGCCTGG ACCAGTTACG GCTAGACACC CGGCTTTCTC CCCACATGCA GAGTAGTGGA 
CATACTCTCA TGCTCAGCAC CTTGGACGCC AAGGAAAATC AGGAGGGAAC ACTGAGGAGA 
CGTTCTCTGA GGCGCAGTAA CAGCATCTCC AAGTCTCCTG GCCCCAGCTC CCCCAAGGAG 
CCGCTGCTCC TGAGCCGAGA CATCAGTCGC TCAGAGTCCC TTCGCTGCTC CAGCAGCTAC 
TCACAGCAGA TCTTCCGGCC CTGTGACCTG ATCCACGGGG AGGTCCTGGG GAAGGGCTTC 
TTCGGACAGG CCATCAAGGT GACTCACAAA GCCACAGGCA AAGTGATGGT CATGAAGGAG 
TTAATTCGCT GTGATGAGGA AACCCAGAAG ACTTTCCTGA CTGAGGTGAA AGTGATGCGA 
AGCCTGGACC ACCCTAACGT GCTTAAGTTC ATCGGGGTCC TGTACAAGGA CAAGAAGCTG 
AATCTGCTGA CAGAGTACAT TGAGGGGGGC ACCCTGAAGG ACTTTCTGCG CAATGTGGAC 
CCGTTTCCCT GGCAACAGAA GGTCAGGTTT GCCAAAGGCA TCGCTTCCGG AATGGCTTAT 
TTACACTCCA TGTGCATCAT CCACCGAGAT CTGAACTCGC ACAACTGTCT CATCAAATTG 
GACAAGACGG TGGTGGTAGC AGACTTCGGG CTGTCACGTC TCATAGTGGA AGAGAGGAAA 
AGGCCTCCAG TAGAGAAGGC CGCCACCAAG AAACGCACCT TACGCAAGAG TGATCGCAAG 
AAGCGCTACA CTGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAATGGCAAG 
AGCTACGATG AGACTGTGGA TGTCTTCTCT TTTGGGATTG TTCTCTGTGA GATCATTGGG 
CAGGTATATG CTGATCCCGA CTGCCTTCCC CGCACACTGG ACTTCGGCCT CAACGTAAAG 
CTTTTCTGGG AGAAGTTTGT CCCAACAGAC TGTCCCCCAG CCTTCTTCCC CCTGGCTGCT 
ATCTGCTGCA AACTGGAGCC TGAGAGCAGA CCGGCATTCT CAAAGCTGGA GGACTCATTC 
GAGGCGCTCT CCCTGTTCTT GGGAGAGTTG GCCATCCCAC TGCCAGCAGA GCTGGAAGAG 
CTGGACCACA CTGTGAGCAT GGAGTATGGC CTAACCCGGG ACTCGCCACC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( NM_024135.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077049.2 Rattus norvegicus LIM domain kinase 2 (Limk2), mRNA.

Price & Availability↑

CloneID	ORa06798
Clone ID Related Accession (Same CDS sequence)	NM_024135.2
Accession Version	NM_024135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D31874.1. On Nov 29, 2004 this sequence version replaced NM_024135.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D31874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGACCGGCAT TCTCAAAGCT GGAGGACTCA 
TTCGAGGCGC TCTCCCTGTT CTTGGGAGAG TTGGCCATCC CACTGCCAGC AGAGCTGGAA 
GAGCTGGACC ACACTGTGAG CATGGAGTAT GGCCTAACCC GGGACTCGCC ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077049.2
CDS	63..1979
Translation	MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEELDHTVSMEYGLTRDSPP

Target ORF information:

RefSeq Version	NM_024135.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024135.2

ORF Insert Sequence:

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGACCGGCAT TCTCAAAGCT GGAGGACTCA 
TTCGAGGCGC TCTCCCTGTT CTTGGGAGAG TTGGCCATCC CACTGCCAGC AGAGCTGGAA 
GAGCTGGACC ACACTGTGAG CATGGAGTAT GGCCTAACCC GGGACTCGCC ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( XM_008770275.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768497.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43299
Clone ID Related Accession (Same CDS sequence)	XM_008770275.2
Accession Version	XM_008770275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGCTCTTCTT TCCAGGAGGA AGCCTTGGGA 
AACCCACCCT TCCCAACCCT GGGAAGCTCC ATCAGCCAAA CCCTGGGGCA CTACAGAGTT 
CAAGACTTGC AGGAATTACT GATTGCTCTG GGTCTACATG CAAGCTGGGC TCGCCAGAGA 
GAAGAGAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768497.1
CDS	167..2098
Translation	MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESSSSFQEEALGNPPFPTLGSSISQTLGHYRVQDLQELLIALGLHASWARQREER

Target ORF information:

RefSeq Version	XM_008770275.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770275.2

ORF Insert Sequence:

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGCTCTTCTT TCCAGGAGGA AGCCTTGGGA 
AACCCACCCT TCCCAACCCT GGGAAGCTCC ATCAGCCAAA CCCTGGGGCA CTACAGAGTT 
CAAGACTTGC AGGAATTACT GATTGCTCTG GGTCTACATG CAAGCTGGGC TCGCCAGAGA 
GAAGAGAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( XM_008770276.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768498.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X3, mRNA.

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CloneID	ORa43300
Clone ID Related Accession (Same CDS sequence)	XM_008770276.2
Accession Version	XM_008770276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCCT GCGGGTATCT GCCAAGGAAC CAGGGGCCTG GAATCGTCTC TCTCCTGCTG 
CTTCGGAGGC AGAGGTGTTC CGAGTGCCAG GAGTCCCTCA CCAACTGGTA CTATGAGAAG 
GACGGGAAGC TCTACTGCCA CAAGGACTAC TGGGCCAAGT TTGGAGAATT CTGCCACGGG 
TGCTCCCTGC TGATGACAGG GCCAGCCATG GTGGCTGGGG AGTTTAAGTA CCACCCAGAG 
TGCTTTGCCT GCATGAGTTG CAAGGTGATC ATCGAGGATG GGGATGCGTA CGCCCTGGTG 
CAGCATGCTA CACTCTACTG CGGGAAGTGC CACAATGAGG TGGTGCTGGC ACCCATGTTT 
GAAAGACTGT CTACGGAGTC TGTCCAGGAC CAGCTGCCCT ACTCTGTCAC GCTCATCTCC 
ATGCCTGCCA CCACCGAGTG CAGACGTGGC TTCTCTGTGT CTGTGGAAAG CGCCTCCTCT 
AACTATGCCA CCACCGTGCA AGTGAAAGAG GTCAACCGGA TGCACATCAG CCCCAACAAC 
CGAAATGCCA TCCACCCCGG GGACCGCATC TTGGAGATCA ATGGGACCCC TGTCCGCACT 
CTCCGAGTAG AAGAGGTGGA GGATGCGATT AACCAGACGA GCCAGACACT TCAACTGCTG 
ATTGAACATG ACCCTGTCCC CCAGCGCCTG GACCAGTTAC GGCTAGACAC CCGGCTTTCT 
CCCCACATGC AGAGTAGTGG ACATACTCTC ATGCTCAGCA CCTTGGACGC CAAGGAAAAT 
CAGGAGGGAA CACTGAGGAG ACGTTCTCTG AGGCGCAGTA ACAGCATCTC CAAGTCTCCT 
GGCCCCAGCT CCCCCAAGGA GCCGCTGCTC CTGAGCCGAG ACATCAGTCG CTCAGAGTCC 
CTTCGCTGCT CCAGCAGCTA CTCACAGCAG ATCTTCCGGC CCTGTGACCT GATCCACGGG 
GAGGTCCTGG GGAAGGGCTT CTTCGGACAG GCCATCAAGG TGACTCACAA AGCCACAGGC 
AAAGTGATGG TCATGAAGGA GTTAATTCGC TGTGATGAGG AAACCCAGAA GACTTTCCTG 
ACTGAGGTGA AAGTGATGCG AAGCCTGGAC CACCCTAACG TGCTTAAGTT CATCGGGGTC 
CTGTACAAGG ACAAGAAGCT GAATCTGCTG ACAGAGTACA TTGAGGGGGG CACCCTGAAG 
GACTTTCTGC GCAATGTGGA CCCGTTTCCC TGGCAACAGA AGGTCAGGTT TGCCAAAGGC 
ATCGCTTCCG GAATGGCTTA TTTACACTCC ATGTGCATCA TCCACCGAGA TCTGAACTCG 
CACAACTGTC TCATCAAATT GGACAAGACG GTGGTGGTAG CAGACTTCGG GCTGTCACGT 
CTCATAGTGG AAGAGAGGAA AAGGCCTCCA GTAGAGAAGG CCGCCACCAA GAAACGCACC 
TTACGCAAGA GTGATCGCAA GAAGCGCTAC ACTGTGGTGG GAAACCCCTA CTGGATGGCC 
CCTGAGATGC TGAATGGCAA GAGCTACGAT GAGACTGTGG ATGTCTTCTC TTTTGGGATT 
GTTCTCTGTG AGATCATTGG GCAGGTATAT GCTGATCCCG ACTGCCTTCC CCGCACACTG 
GACTTCGGCC TCAACGTAAA GCTTTTCTGG GAGAAGTTTG TCCCAACAGA CTGTCCCCCA 
GCCTTCTTCC CCCTGGCTGC TATCTGCTGC AAACTGGAGC CTGAGAGCAG CTCTTCTTTC 
CAGGAGGAAG CCTTGGGAAA CCCACCCTTC CCAACCCTGG GAAGCTCCAT CAGCCAAACC 
CTGGGGCACT ACAGAGTTCA AGACTTGCAG GAATTACTGA TTGCTCTGGG TCTACATGCA 
AGCTGGGCTC GCCAGAGAGA AGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768498.1
CDS	1246..3135
Translation	MCPCGYLPRNQGPGIVSLLLLRRQRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESSSSFQEEALGNPPFPTLGSSISQTLGHYRVQDLQELLIALGLHASWARQREER

Target ORF information:

RefSeq Version	XM_008770276.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770276.2

ORF Insert Sequence:

ATGTGCCCCT GCGGGTATCT GCCAAGGAAC CAGGGGCCTG GAATCGTCTC TCTCCTGCTG 
CTTCGGAGGC AGAGGTGTTC CGAGTGCCAG GAGTCCCTCA CCAACTGGTA CTATGAGAAG 
GACGGGAAGC TCTACTGCCA CAAGGACTAC TGGGCCAAGT TTGGAGAATT CTGCCACGGG 
TGCTCCCTGC TGATGACAGG GCCAGCCATG GTGGCTGGGG AGTTTAAGTA CCACCCAGAG 
TGCTTTGCCT GCATGAGTTG CAAGGTGATC ATCGAGGATG GGGATGCGTA CGCCCTGGTG 
CAGCATGCTA CACTCTACTG CGGGAAGTGC CACAATGAGG TGGTGCTGGC ACCCATGTTT 
GAAAGACTGT CTACGGAGTC TGTCCAGGAC CAGCTGCCCT ACTCTGTCAC GCTCATCTCC 
ATGCCTGCCA CCACCGAGTG CAGACGTGGC TTCTCTGTGT CTGTGGAAAG CGCCTCCTCT 
AACTATGCCA CCACCGTGCA AGTGAAAGAG GTCAACCGGA TGCACATCAG CCCCAACAAC 
CGAAATGCCA TCCACCCCGG GGACCGCATC TTGGAGATCA ATGGGACCCC TGTCCGCACT 
CTCCGAGTAG AAGAGGTGGA GGATGCGATT AACCAGACGA GCCAGACACT TCAACTGCTG 
ATTGAACATG ACCCTGTCCC CCAGCGCCTG GACCAGTTAC GGCTAGACAC CCGGCTTTCT 
CCCCACATGC AGAGTAGTGG ACATACTCTC ATGCTCAGCA CCTTGGACGC CAAGGAAAAT 
CAGGAGGGAA CACTGAGGAG ACGTTCTCTG AGGCGCAGTA ACAGCATCTC CAAGTCTCCT 
GGCCCCAGCT CCCCCAAGGA GCCGCTGCTC CTGAGCCGAG ACATCAGTCG CTCAGAGTCC 
CTTCGCTGCT CCAGCAGCTA CTCACAGCAG ATCTTCCGGC CCTGTGACCT GATCCACGGG 
GAGGTCCTGG GGAAGGGCTT CTTCGGACAG GCCATCAAGG TGACTCACAA AGCCACAGGC 
AAAGTGATGG TCATGAAGGA GTTAATTCGC TGTGATGAGG AAACCCAGAA GACTTTCCTG 
ACTGAGGTGA AAGTGATGCG AAGCCTGGAC CACCCTAACG TGCTTAAGTT CATCGGGGTC 
CTGTACAAGG ACAAGAAGCT GAATCTGCTG ACAGAGTACA TTGAGGGGGG CACCCTGAAG 
GACTTTCTGC GCAATGTGGA CCCGTTTCCC TGGCAACAGA AGGTCAGGTT TGCCAAAGGC 
ATCGCTTCCG GAATGGCTTA TTTACACTCC ATGTGCATCA TCCACCGAGA TCTGAACTCG 
CACAACTGTC TCATCAAATT GGACAAGACG GTGGTGGTAG CAGACTTCGG GCTGTCACGT 
CTCATAGTGG AAGAGAGGAA AAGGCCTCCA GTAGAGAAGG CCGCCACCAA GAAACGCACC 
TTACGCAAGA GTGATCGCAA GAAGCGCTAC ACTGTGGTGG GAAACCCCTA CTGGATGGCC 
CCTGAGATGC TGAATGGCAA GAGCTACGAT GAGACTGTGG ATGTCTTCTC TTTTGGGATT 
GTTCTCTGTG AGATCATTGG GCAGGTATAT GCTGATCCCG ACTGCCTTCC CCGCACACTG 
GACTTCGGCC TCAACGTAAA GCTTTTCTGG GAGAAGTTTG TCCCAACAGA CTGTCCCCCA 
GCCTTCTTCC CCCTGGCTGC TATCTGCTGC AAACTGGAGC CTGAGAGCAG CTCTTCTTTC 
CAGGAGGAAG CCTTGGGAAA CCCACCCTTC CCAACCCTGG GAAGCTCCAT CAGCCAAACC 
CTGGGGCACT ACAGAGTTCA AGACTTGCAG GAATTACTGA TTGCTCTGGG TCTACATGCA 
AGCTGGGCTC GCCAGAGAGA AGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( XM_008770277.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768499.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa43301
Clone ID Related Accession (Same CDS sequence)	XM_008770277.2
Accession Version	XM_008770277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTT ACTTGTCTGT CCCGGCTTAC TTCACCTCCA GAGACCCCTT TAGGTGTTCC 
GAGTGCCAGG AGTCCCTCAC CAACTGGTAC TATGAGAAGG ACGGGAAGCT CTACTGCCAC 
AAGGACTACT GGGCCAAGTT TGGAGAATTC TGCCACGGGT GCTCCCTGCT GATGACAGGG 
CCAGCCATGG TGGCTGGGGA GTTTAAGTAC CACCCAGAGT GCTTTGCCTG CATGAGTTGC 
AAGGTGATCA TCGAGGATGG GGATGCGTAC GCCCTGGTGC AGCATGCTAC ACTCTACTGC 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AAAGACTGTC TACGGAGTCT 
GTCCAGGACC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCTGCCAC CACCGAGTGC 
AGACGTGGCT TCTCTGTGTC TGTGGAAAGC GCCTCCTCTA ACTATGCCAC CACCGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGC CCCAACAACC GAAATGCCAT CCACCCCGGG 
GACCGCATCT TGGAGATCAA TGGGACCCCT GTCCGCACTC TCCGAGTAGA AGAGGTGGAG 
GATGCGATTA ACCAGACGAG CCAGACACTT CAACTGCTGA TTGAACATGA CCCTGTCCCC 
CAGCGCCTGG ACCAGTTACG GCTAGACACC CGGCTTTCTC CCCACATGCA GAGTAGTGGA 
CATACTCTCA TGCTCAGCAC CTTGGACGCC AAGGAAAATC AGGAGGGAAC ACTGAGGAGA 
CGTTCTCTGA GGCGCAGTAA CAGCATCTCC AAGTCTCCTG GCCCCAGCTC CCCCAAGGAG 
CCGCTGCTCC TGAGCCGAGA CATCAGTCGC TCAGAGTCCC TTCGCTGCTC CAGCAGCTAC 
TCACAGCAGA TCTTCCGGCC CTGTGACCTG ATCCACGGGG AGGTCCTGGG GAAGGGCTTC 
TTCGGACAGG CCATCAAGGT GACTCACAAA GCCACAGGCA AAGTGATGGT CATGAAGGAG 
TTAATTCGCT GTGATGAGGA AACCCAGAAG ACTTTCCTGA CTGAGGTGAA AGTGATGCGA 
AGCCTGGACC ACCCTAACGT GCTTAAGTTC ATCGGGGTCC TGTACAAGGA CAAGAAGCTG 
AATCTGCTGA CAGAGTACAT TGAGGGGGGC ACCCTGAAGG ACTTTCTGCG CAATGTGGAC 
CCGTTTCCCT GGCAACAGAA GGTCAGGTTT GCCAAAGGCA TCGCTTCCGG AATGGCTTAT 
TTACACTCCA TGTGCATCAT CCACCGAGAT CTGAACTCGC ACAACTGTCT CATCAAATTG 
GACAAGACGG TGGTGGTAGC AGACTTCGGG CTGTCACGTC TCATAGTGGA AGAGAGGAAA 
AGGCCTCCAG TAGAGAAGGC CGCCACCAAG AAACGCACCT TACGCAAGAG TGATCGCAAG 
AAGCGCTACA CTGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAATGGCAAG 
AGCTACGATG AGACTGTGGA TGTCTTCTCT TTTGGGATTG TTCTCTGTGA GATCATTGGG 
CAGGTATATG CTGATCCCGA CTGCCTTCCC CGCACACTGG ACTTCGGCCT CAACGTAAAG 
CTTTTCTGGG AGAAGTTTGT CCCAACAGAC TGTCCCCCAG CCTTCTTCCC CCTGGCTGCT 
ATCTGCTGCA AACTGGAGCC TGAGAGCAGC TCTTCTTTCC AGGAGGAAGC CTTGGGAAAC 
CCACCCTTCC CAACCCTGGG AAGCTCCATC AGCCAAACCC TGGGGCACTA CAGAGTTCAA 
GACTTGCAGG AATTACTGAT TGCTCTGGGT CTACATGCAA GCTGGGCTCG CCAGAGAGAA 
GAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768499.1
CDS	1449..3317
Translation	MGSYLSVPAYFTSRDPFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESSSSFQEEALGNPPFPTLGSSISQTLGHYRVQDLQELLIALGLHASWARQREER

Target ORF information:

RefSeq Version	XM_008770277.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770277.2

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCTGT CCCGGCTTAC TTCACCTCCA GAGACCCCTT TAGGTGTTCC 
GAGTGCCAGG AGTCCCTCAC CAACTGGTAC TATGAGAAGG ACGGGAAGCT CTACTGCCAC 
AAGGACTACT GGGCCAAGTT TGGAGAATTC TGCCACGGGT GCTCCCTGCT GATGACAGGG 
CCAGCCATGG TGGCTGGGGA GTTTAAGTAC CACCCAGAGT GCTTTGCCTG CATGAGTTGC 
AAGGTGATCA TCGAGGATGG GGATGCGTAC GCCCTGGTGC AGCATGCTAC ACTCTACTGC 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AAAGACTGTC TACGGAGTCT 
GTCCAGGACC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCTGCCAC CACCGAGTGC 
AGACGTGGCT TCTCTGTGTC TGTGGAAAGC GCCTCCTCTA ACTATGCCAC CACCGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGC CCCAACAACC GAAATGCCAT CCACCCCGGG 
GACCGCATCT TGGAGATCAA TGGGACCCCT GTCCGCACTC TCCGAGTAGA AGAGGTGGAG 
GATGCGATTA ACCAGACGAG CCAGACACTT CAACTGCTGA TTGAACATGA CCCTGTCCCC 
CAGCGCCTGG ACCAGTTACG GCTAGACACC CGGCTTTCTC CCCACATGCA GAGTAGTGGA 
CATACTCTCA TGCTCAGCAC CTTGGACGCC AAGGAAAATC AGGAGGGAAC ACTGAGGAGA 
CGTTCTCTGA GGCGCAGTAA CAGCATCTCC AAGTCTCCTG GCCCCAGCTC CCCCAAGGAG 
CCGCTGCTCC TGAGCCGAGA CATCAGTCGC TCAGAGTCCC TTCGCTGCTC CAGCAGCTAC 
TCACAGCAGA TCTTCCGGCC CTGTGACCTG ATCCACGGGG AGGTCCTGGG GAAGGGCTTC 
TTCGGACAGG CCATCAAGGT GACTCACAAA GCCACAGGCA AAGTGATGGT CATGAAGGAG 
TTAATTCGCT GTGATGAGGA AACCCAGAAG ACTTTCCTGA CTGAGGTGAA AGTGATGCGA 
AGCCTGGACC ACCCTAACGT GCTTAAGTTC ATCGGGGTCC TGTACAAGGA CAAGAAGCTG 
AATCTGCTGA CAGAGTACAT TGAGGGGGGC ACCCTGAAGG ACTTTCTGCG CAATGTGGAC 
CCGTTTCCCT GGCAACAGAA GGTCAGGTTT GCCAAAGGCA TCGCTTCCGG AATGGCTTAT 
TTACACTCCA TGTGCATCAT CCACCGAGAT CTGAACTCGC ACAACTGTCT CATCAAATTG 
GACAAGACGG TGGTGGTAGC AGACTTCGGG CTGTCACGTC TCATAGTGGA AGAGAGGAAA 
AGGCCTCCAG TAGAGAAGGC CGCCACCAAG AAACGCACCT TACGCAAGAG TGATCGCAAG 
AAGCGCTACA CTGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAATGGCAAG 
AGCTACGATG AGACTGTGGA TGTCTTCTCT TTTGGGATTG TTCTCTGTGA GATCATTGGG 
CAGGTATATG CTGATCCCGA CTGCCTTCCC CGCACACTGG ACTTCGGCCT CAACGTAAAG 
CTTTTCTGGG AGAAGTTTGT CCCAACAGAC TGTCCCCCAG CCTTCTTCCC CCTGGCTGCT 
ATCTGCTGCA AACTGGAGCC TGAGAGCAGC TCTTCTTTCC AGGAGGAAGC CTTGGGAAAC 
CCACCCTTCC CAACCCTGGG AAGCTCCATC AGCCAAACCC TGGGGCACTA CAGAGTTCAA 
GACTTGCAGG AATTACTGAT TGCTCTGGGT CTACATGCAA GCTGGGCTCG CCAGAGAGAA 
GAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( XM_008770278.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768500.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X4, mRNA.

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CloneID	ORa43302
Clone ID Related Accession (Same CDS sequence)	XM_008770278.2
Accession Version	XM_008770278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGGAGGAAGC CTTGGGAAAC CCACCCTTCC 
CAACCCTGGG AAGCTCCATC AGCCAAACCC TGGGGCACTA CAGAGTTCAA GACTTGCAGG 
AATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768500.1
CDS	166..2034
Translation	MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRRKPWETHPSQPWEAPSAKPWGTTEFKTCRNY

Target ORF information:

RefSeq Version	XM_008770278.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770278.2

ORF Insert Sequence:

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGGAGGAAGC CTTGGGAAAC CCACCCTTCC 
CAACCCTGGG AAGCTCCATC AGCCAAACCC TGGGGCACTA CAGAGTTCAA GACTTGCAGG 
AATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limk2 ( XM_008770279.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768501.1 Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa43303
Clone ID Related Accession (Same CDS sequence)	XM_008770279.2
Accession Version	XM_008770279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGGAAAATCT GGACGAAGCA TGTGGACCCA 
TTGATAGAAT GTGTGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768501.1
CDS	166..1986
Translation	MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRKIWTKHVDPLIECVR

Target ORF information:

RefSeq Version	XM_008770279.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain kinase 2 (Limk2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770279.2

ORF Insert Sequence:

ATGGCGGCGC TGGCGGGTGA AGAGGCCTGG AGGTGTCGGG GCTGTGGCAA CTATGTTCCC 
CTGAGCCAGA GGCTGTACAG GACAGCCAAC GAGGCCTGGC ATAGCTCCTG CTTCCGGTGT 
TCCGAGTGCC AGGAGTCCCT CACCAACTGG TACTATGAGA AGGACGGGAA GCTCTACTGC 
CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCACG GGTGCTCCCT GCTGATGACA 
GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT 
TGCAAGGTGA TCATCGAGGA TGGGGATGCG TACGCCCTGG TGCAGCATGC TACACTCTAC 
TGCGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACGGAG 
TCTGTCCAGG ACCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC CACCACCGAG 
TGCAGACGTG GCTTCTCTGT GTCTGTGGAA AGCGCCTCCT CTAACTATGC CACCACCGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGCCCCAACA ACCGAAATGC CATCCACCCC 
GGGGACCGCA TCTTGGAGAT CAATGGGACC CCTGTCCGCA CTCTCCGAGT AGAAGAGGTG 
GAGGATGCGA TTAACCAGAC GAGCCAGACA CTTCAACTGC TGATTGAACA TGACCCTGTC 
CCCCAGCGCC TGGACCAGTT ACGGCTAGAC ACCCGGCTTT CTCCCCACAT GCAGAGTAGT 
GGACATACTC TCATGCTCAG CACCTTGGAC GCCAAGGAAA ATCAGGAGGG AACACTGAGG 
AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CTGGCCCCAG CTCCCCCAAG 
GAGCCGCTGC TCCTGAGCCG AGACATCAGT CGCTCAGAGT CCCTTCGCTG CTCCAGCAGC 
TACTCACAGC AGATCTTCCG GCCCTGTGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC 
TTCTTCGGAC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG 
GAGTTAATTC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG 
CGAAGCCTGG ACCACCCTAA CGTGCTTAAG TTCATCGGGG TCCTGTACAA GGACAAGAAG 
CTGAATCTGC TGACAGAGTA CATTGAGGGG GGCACCCTGA AGGACTTTCT GCGCAATGTG 
GACCCGTTTC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCGCTTC CGGAATGGCT 
TATTTACACT CCATGTGCAT CATCCACCGA GATCTGAACT CGCACAACTG TCTCATCAAA 
TTGGACAAGA CGGTGGTGGT AGCAGACTTC GGGCTGTCAC GTCTCATAGT GGAAGAGAGG 
AAAAGGCCTC CAGTAGAGAA GGCCGCCACC AAGAAACGCA CCTTACGCAA GAGTGATCGC 
AAGAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAATGGC 
AAGAGCTACG ATGAGACTGT GGATGTCTTC TCTTTTGGGA TTGTTCTCTG TGAGATCATT 
GGGCAGGTAT ATGCTGATCC CGACTGCCTT CCCCGCACAC TGGACTTCGG CCTCAACGTA 
AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT 
GCTATCTGCT GCAAACTGGA GCCTGAGAGC AGGAAAATCT GGACGAAGCA TGTGGACCCA 
TTGATAGAAT GTGTGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Developmental biology415(2)216-227(2016 07)
Palmer K,Fairfield H,Borgeia S,Curtain M,Hassan MG,Dionne L,Yong Karst S,Coombs H,Bronson RT,Reinholdt LG,Bergstrom DE,Donahue LR,Cox TC,Murray SA

Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.
Molecular brain858(2015 Oct)
Ko AR,Hyun HW,Min SJ,Kim JE,Kang TC

LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission.
Cell death and differentiation21(7)1036-49(2014 Jul)
Kim JE,Ryu HJ,Kim MJ,Kang TC

LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.
Science signaling7(331)ra61(2014 Jun)
Li Y,Hu F,Chen HJ,Du YJ,Xie ZY,Zhang Y,Wang J,Wang Y

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Limk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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