Dap3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dap3
Entrez Gene ID	295238
Full Name	death associated protein 3
General protein information	Preferred Names death associated protein 3 Names 28S ribosomal protein S29, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232610.3	XP_006232672.1	28S ribosomal protein S29, mitochondrial isoform X1
NM_001011950.2	NP_001011950.2	28S ribosomal protein S29, mitochondrial
XM_006232611.2	XP_006232673.1	28S ribosomal protein S29, mitochondrial isoform X2

Pathways from BioSystems

Homologies

	DAP3	XP_002801886.1
Canis lupus familiaris (dog)	DAP3	XP_537250.1
Drosophila melanogaster (fruit fly)	mRpS29	NP_523811.1
Caenorhabditis elegans (roundworm)	dap-3	NP_496280.1
Danio rerio (zebrafish)	dap3	NP_001092207.1
Xenopus tropicalis (tropical clawed frog)	dap3	NP_001016002.1
Mus musculus (house mouse)	Dap3	NP_075370.2
Homo sapiens (human)	DAP3	NP_001186778.1
Bos taurus (cattle)	DAP3	NP_001106765.1
Rattus norvegicus (Norway rat)	Dap3	NP_001011950.2
Gallus gallus (chicken)	DAP3	XP_004948343.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000890	XP_316867.5

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Identification of DELE, a novel DAP3-binding protein which is crucial for death receptor-mediated apoptosis induction.
Harada T, Iwai A, Miyazaki T
Apoptosis : an international journal on programmed cell death15(10)1247-55(2010 Oct)

Mammalian dap3 is an essential gene required for mitochondrial homeostasis in vivo and contributing to the extrinsic pathway for apoptosis.
Kim HR, Chae HJ, Thomas M, Miyazaki T, Monosov A, Monosov E, Krajewska M, Krajewski S, Reed JC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology21(1)188-96(2007 Jan)

The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins.
Berger T, Brigl M, Herrmann JM, Vielhauer V, Luckow B, Schl?ndorff D, Kretzler M
Journal of cell science113 ( Pt 20)3603-12(2000 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional characterization of a similar protein in mouse.
Title: Mammalian dap3 is an essential gene required for mitochondrial homeostasis in vivo and contributing to the extrinsic pathway for apoptosis.

The following Dap3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dap3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01698	NM_001011950.2 Latest version!	Rattus norvegicus death associated protein 3 (Dap3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa24847	XM_006232610.3 Latest version!	Rattus norvegicus death associated protein 3 (Dap3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa24848	XM_006232611.2 Latest version!	Rattus norvegicus death associated protein 3 (Dap3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dap3 ( NM_001011950.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001011950.2 Rattus norvegicus death associated protein 3 (Dap3), mRNA.

Price & Availability↑

CloneID	ORa01698
Clone ID Related Accession (Same CDS sequence)	NM_001011950.2
Accession Version	NM_001011950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Rattus norvegicus(Norway rat)
Product	28S ribosomal protein S29, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085878.1 and AA964664.1. On Sep 1, 2009 this sequence version replaced NM_001011950.1. ##Evidence-Data-START## Transcript exon combination :: BC085878.1, FQ230674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGATT 
GCCCCGGAGG AACTCGCCCT TGTCCGCAAC CTAAGAAAAA TGGTGAAAAA TGACTGGCAC 
GGAGGAGCAA TTGTGCTGAG CTTGAGCCAA ACCGGGTCTC TCTTCAAGTC CAGAACAGCC 
TATTTGCCAC ATGAGCTGCT GGGAAAGGAA GGATTTAATG CCCTGGAGCC TTTTCTTCCC 
ATCCTCATTT CCAACTATAA TCCAAAGGAA TTTGAAAGTT CCTTTCAGTA TTACTTGGAG 
AACAACTGGC TTCAGCATGA GAAAGCTTCA ACAGAGGAAG GGAGGAAGGA GCTGAAGTTC 
CTGAGTAACT GCAACCCTGA GCAGCTAGAG CGGCTCTGCG CCTCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011950.2
CDS	310..1500
Translation	MRTKTMLTGMTRFFSRVQKLDPRCFLHVSVQAPQNNQVPAERPRAVSRTSDSDPAKHGEQHEGQHYSIPPQDLKTVFPHGLPPRYAMQVKTFGEACLMVRKPALELLGYLKNTNFAHPAVRYLLYGEKGTGKTLSLCHAVHFCAKSDWLILHIPDAHLWVKNCGELLQSTYNKQRFDQPLEASTWLKNFKTTNERFLGQIKVQEKYVWNKRESTEKGSPLGEVVEQGLTRVRNATDAVGVVLKELKTQSSLGLFHLLVAVDGVNALWGRTTLKREDRTLIAPEELALVRNLRKMVKNDWHGGAIVLSLSQTGSLFKSRTAYLPHELLGKEGFNALEPFLPILISNYNPKEFESSFQYYLENNWLQHEKASTEEGRKELKFLSNCNPEQLERLCASL

Target ORF information:

RefSeq Version	NM_001011950.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein 3 (Dap3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011950.2

ORF Insert Sequence:

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGATT 
GCCCCGGAGG AACTCGCCCT TGTCCGCAAC CTAAGAAAAA TGGTGAAAAA TGACTGGCAC 
GGAGGAGCAA TTGTGCTGAG CTTGAGCCAA ACCGGGTCTC TCTTCAAGTC CAGAACAGCC 
TATTTGCCAC ATGAGCTGCT GGGAAAGGAA GGATTTAATG CCCTGGAGCC TTTTCTTCCC 
ATCCTCATTT CCAACTATAA TCCAAAGGAA TTTGAAAGTT CCTTTCAGTA TTACTTGGAG 
AACAACTGGC TTCAGCATGA GAAAGCTTCA ACAGAGGAAG GGAGGAAGGA GCTGAAGTTC 
CTGAGTAACT GCAACCCTGA GCAGCTAGAG CGGCTCTGCG CCTCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dap3 ( XM_006232610.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232672.1 Rattus norvegicus death associated protein 3 (Dap3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa24847
Clone ID Related Accession (Same CDS sequence)	XM_006232610.3
Accession Version	XM_006232610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	28S ribosomal protein S29, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232610.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGATT 
GCCCCGGAGG AACTCGCCCT TGTCCGCAAC CTAAGAAAAA TGGTGAAAAA TGACTGGGAA 
GGATTTAATG CCCTGGAGCC TTTTCTTCCC ATCCTCATTT CCAACTATAA TCCAAAGGAA 
TTTGAAAGTT CCTTTCAGTA TTACTTGGAG AACAACTGGC TTCAGCATGA GAAAGCTTCA 
ACAGAGGAAG GGAGGAAGGA GCTGAAGTTC CTGAGTAACT GCAACCCTGA GCAGCTAGAG 
CGGCTCTGCG CCTCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232672.1
CDS	106..1206
Translation	MRTKTMLTGMTRFFSRVQKLDPRCFLHVSVQAPQNNQVPAERPRAVSRTSDSDPAKHGEQHEGQHYSIPPQDLKTVFPHGLPPRYAMQVKTFGEACLMVRKPALELLGYLKNTNFAHPAVRYLLYGEKGTGKTLSLCHAVHFCAKSDWLILHIPDAHLWVKNCGELLQSTYNKQRFDQPLEASTWLKNFKTTNERFLGQIKVQEKYVWNKRESTEKGSPLGEVVEQGLTRVRNATDAVGVVLKELKTQSSLGLFHLLVAVDGVNALWGRTTLKREDRTLIAPEELALVRNLRKMVKNDWEGFNALEPFLPILISNYNPKEFESSFQYYLENNWLQHEKASTEEGRKELKFLSNCNPEQLERLCASL

Target ORF information:

RefSeq Version	XM_006232610.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein 3 (Dap3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232610.3

ORF Insert Sequence:

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGATT 
GCCCCGGAGG AACTCGCCCT TGTCCGCAAC CTAAGAAAAA TGGTGAAAAA TGACTGGGAA 
GGATTTAATG CCCTGGAGCC TTTTCTTCCC ATCCTCATTT CCAACTATAA TCCAAAGGAA 
TTTGAAAGTT CCTTTCAGTA TTACTTGGAG AACAACTGGC TTCAGCATGA GAAAGCTTCA 
ACAGAGGAAG GGAGGAAGGA GCTGAAGTTC CTGAGTAACT GCAACCCTGA GCAGCTAGAG 
CGGCTCTGCG CCTCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dap3 ( XM_006232611.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232673.1 Rattus norvegicus death associated protein 3 (Dap3), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa24848
Clone ID Related Accession (Same CDS sequence)	XM_006232611.2
Accession Version	XM_006232611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	28S ribosomal protein S29, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006232611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGACA 
GAAACCTACC CAGCACGTGC TGCTGCCACC TCACCATGCC TGCCCGCCTC ACCGTACCTG 
CCCGCCTCAC CATGCGTCGC AAAGCAAGGG CACAGATTGC CCCGGAGGAA CTCGCCCTTG 
TCCGCAACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232673.1
CDS	106..1077
Translation	MRTKTMLTGMTRFFSRVQKLDPRCFLHVSVQAPQNNQVPAERPRAVSRTSDSDPAKHGEQHEGQHYSIPPQDLKTVFPHGLPPRYAMQVKTFGEACLMVRKPALELLGYLKNTNFAHPAVRYLLYGEKGTGKTLSLCHAVHFCAKSDWLILHIPDAHLWVKNCGELLQSTYNKQRFDQPLEASTWLKNFKTTNERFLGQIKVQEKYVWNKRESTEKGSPLGEVVEQGLTRVRNATDAVGVVLKELKTQSSLGLFHLLVAVDGVNALWGRTTLKREDRTLTETYPARAAATSPCLPASPYLPASPCVAKQGHRLPRRNSPLSAT

Target ORF information:

RefSeq Version	XM_006232611.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein 3 (Dap3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232611.2

ORF Insert Sequence:

ATGAGAACAA AGACCATGCT GACAGGAATG ACAAGGTTTT TCTCCAGGGT CCAGAAGTTG 
GACCCGAGAT GTTTTTTGCA CGTGAGTGTC CAAGCACCTC AGAACAATCA GGTTCCAGCT 
GAGCGTCCGA GAGCTGTTTC CCGCACCAGT GACAGTGACC CAGCTAAGCA CGGGGAGCAG 
CATGAGGGTC AGCACTACAG CATCCCCCCA CAGGATCTGA AGACGGTGTT TCCTCACGGC 
CTGCCTCCTC GATACGCAAT GCAGGTGAAG ACTTTTGGTG AAGCTTGTCT GATGGTTAGG 
AAGCCAGCTC TCGAGCTTCT GGGCTACCTG AAAAATACCA ATTTTGCTCA TCCAGCTGTA 
CGATATCTTC TCTATGGAGA GAAGGGGACA GGAAAGACCC TCAGTCTCTG TCATGCTGTT 
CATTTCTGTG CAAAAAGTGA CTGGCTGATT CTGCACATCC CAGATGCTCA TCTTTGGGTA 
AAAAACTGTG GGGAGCTGCT ACAGTCCACT TACAACAAAC AGCGCTTTGA CCAACCCTTA 
GAGGCCTCTA CCTGGCTGAA GAATTTCAAA ACTACCAACG AGCGCTTTCT GGGTCAGATA 
AAAGTTCAAG AGAAGTATGT TTGGAATAAG AGAGAAAGCA CTGAGAAAGG CAGTCCTCTG 
GGAGAAGTTG TCGAACAGGG CCTAACGCGA GTGAGAAACG CCACTGACGC TGTTGGGGTT 
GTGCTGAAGG AGCTGAAGAC GCAGAGCTCC CTAGGACTCT TTCACCTCCT GGTGGCTGTG 
GATGGAGTCA ACGCCCTCTG GGGAAGGACC ACGCTGAAAA GAGAAGATAG AACCTTGACA 
GAAACCTACC CAGCACGTGC TGCTGCCACC TCACCATGCC TGCCCGCCTC ACCGTACCTG 
CCCGCCTCAC CATGCGTCGC AAAGCAAGGG CACAGATTGC CCCGGAGGAA CTCGCCCTTG 
TCCGCAACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Identification of DELE, a novel DAP3-binding protein which is crucial for death receptor-mediated apoptosis induction.
Apoptosis : an international journal on programmed cell death15(10)1247-55(2010 Oct)
Harada T,Iwai A,Miyazaki T

Mammalian dap3 is an essential gene required for mitochondrial homeostasis in vivo and contributing to the extrinsic pathway for apoptosis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology21(1)188-96(2007 Jan)
Kim HR,Chae HJ,Thomas M,Miyazaki T,Monosov A,Monosov E,Krajewska M,Krajewski S,Reed JC

The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins.
Journal of cell science113 ( Pt 20)3603-12(2000 Oct)
Berger T,Brigl M,Herrmann JM,Vielhauer V,Luckow B,Schl?ndorff D,Kretzler M

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Dap3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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