Shh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shh
Entrez Gene ID	29499
Full Name	sonic hedgehog
Synonyms	ShhNC
General protein information	Preferred Names sonic hedgehog Names sonic hedgehog protein shh unprocessed N-terminal signaling and C-terminal autoprocessing domains sonic hedgehog homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q11
Summary	plays a role in neurogenesis; regulates proliferation of adult hippocampal neural stem cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017221.1	NP_058917.1	sonic hedgehog protein precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	shh	XP_002932544.2
Homo sapiens (human)	SHH	NP_000184.1
Mus musculus (house mouse)	Shh	NP_033196.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001412	XP_321721.5
Bos taurus (cattle)	SHH	XP_002687031.2
Rattus norvegicus (Norway rat)	Shh	NP_058917.1
Gallus gallus (chicken)	SHH	NP_990152.1
Danio rerio (zebrafish)	shha	NP_571138.1
Danio rerio (zebrafish)	shhb	NP_571274.2
	SHH	XP_001106515.2
Canis lupus familiaris (dog)	SHH	XP_850450.1
Pan troglodytes (chimpanzee)	SHH	XP_001147185.2
Drosophila melanogaster (fruit fly)	hh	NP_001034065.1

Gene Ontology
Bibliography

Related articles in PubMed

CGRP attenuates hyperoxia-induced oxidative stress-related injury to alveolar epithelial type?II cells via the activation of the Sonic hedgehog pathway.
Dang HX, Li J, Liu C, Fu Y, Zhou F, Tang L, Li L, Xu F
International journal of molecular medicine40(1)209-216(2017 Jul)

High frequency stimulation induces sonic hedgehog release from hippocampal neurons.
Su Y, Yuan Y, Feng S, Ma S, Wang Y
Scientific reports743865(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a positive role for Shh signaling, through the activator Smo, in oligodendrocyte differentiation after lineage specification.
Title: Role of Sonic Hedgehog Signaling in Oligodendrocyte Differentiation.

Tissue samples from fibrotic kidneys show enhanced TGF-beta1 levels, as well as upregulated hedgehog signaling activity, during the epithelial-mesenchymal transition process; these levels decrease when fibrosis is reversed.
Title: Transforming growth factor-?1 stimulates hedgehog signaling to promote epithelial-mesenchymal transition after kidney injury.

the results provide critical insights into the newly discovered role of Shh in phenotypic modulation of vascular smooth muscle cells which depends on KLF4.
Title: Shh mediates PDGF-induced contractile-to-synthetic phenotypic modulation in vascular smooth muscle cells through regulation of KLF4.

the activation of the SHH signaling pathway promotes the induction of EMT and renal tubulointerstitial fibrosis.
Title: Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis.

SHH over expression may play a pivotal contribute role in vasculogenesis through activating the SHH signals in post-myocardial ischemic-reperfusion injury
Title: Activation of SHH signaling pathway promotes vasculogenesis in post-myocardial ischemic-reperfusion injury.

The following Shh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13511	NM_017221.1 Latest version!	Rattus norvegicus sonic hedgehog signaling molecule (Shh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shh ( NM_017221.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058917.1 Rattus norvegicus sonic hedgehog signaling molecule (Shh), mRNA.

Price & Availability↑

CloneID	ORa13511
Clone ID Related Accession (Same CDS sequence)	NM_017221.1
Accession Version	NM_017221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Rattus norvegicus(Norway rat)
Product	sonic hedgehog protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L27340.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27340.1, EV771078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC TGCTGGCCAG ATGTTTTCTG GTGGCCCTTG CTTCCTCGCT GCTGGTGTGC 
CCCGGACTGG CCTGTGGGCC CGGCAGGGGG TTTGGAAAGA GGCAGCACCC CAAAAAGCTG 
ACCCCTTTAG CCTACAAGCA GTTTATCCCC AACGTAGCCG AGAAGACCCT AGGGGCCAGC 
GGCCGATATG AAGGGAAGAT CACAAGAAAC TCCGAACGAT TTAAGGAACT CACCCCCAAT 
TACAACCCCG ACATCATATT TAAGGATGAG GAAAACACTG GAGCAGACCG GCTGATGACT 
CAGAGGTGCA AAGACAAGTT AAATGCCTTG GCCATCTCCG TGATGAACCA GTGGCCTGGA 
GTGAAGCTTC GAGTGACTGA GGGCTGGGAT GAGGACGGCC ATCATTCAGA GGAGTCTCTA 
CACTATGAGG GTCGAGCAGT GGACATCACC ACGTCTGACA GGGACCGCAG CAAGTATGGC 
ATGCTGGCTC GCCTGGCTGT GGAGGCTGGA TTCGACTGGG TCTACTATGA ATCCAAAGCT 
CGCATCCACT GCTCTGTGAA AGCAGAGAAC TCCGTGGCGG CCAAATCTGA CGGCTGCTTC 
CCGGGATCAG CCACAGTGCA CCTGGAGCAG GGTGGCACCA AGTTAGTGAA GGATCTAAGT 
CCCGGGGACC GCGTGCTGGC GGCTGACGAC CAGGGCCGGC TGCTGTACAG CGACTTCCTC 
ACCTTCCTGG ACCGCGACGA AGGTGCCAAG AAGGTCTTCT ACGTGATCGA GACGCGGGAG 
CCGCGGGAGC GTCTGCTGCT CACTGCCGCG CACCTGCTCT TCGTGGCGCC GCACAACGAC 
TCCGGGCCCA CTCCGGGACC GAGCCCACTC TTCGCCAGCC GCGTGCGTCC GGGGCAGCGC 
GTGTACGTGG TGGCTGAACG CGGCGGGGAC CGCCGGCTGC TGCCCGCCGC GGTGCACAGC 
GTAACGCTAC GAGAGGAGGC GGCGGGTGCG TACGCGCCGC TCACGGCGGA CGGCACCATT 
CTCATCAACC GGGTGCTCGC CTCGTGCTAC GCAGTCATCG AGGAGCACAG CTGGGCACAC 
CGGGCCTTCG CGCCCTTCCG CCTGGCGCAC GCGCTGCTGG CCGCGCTGGC ACCCGCCCGC 
ACGGACGGCG GGGGCGGGGG CAGCATCCCT GCCCCGCAAT CTGTAGCGGA AGCGAGGGGC 
GCAGGGCCGC CTGCGGGCAT CCACTGGTAC TCGCAGCTGC TGTACCACAT TGGCACCTGG 
CTGTTGGACA GCGAGACCCT GCATCCCTTG GGAATGGCAG TCAAGTCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058917.1
CDS	315..1628
Translation	MLLLLARCFLVALASSLLVCPGLACGPGRGFGKRQHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKARIHCSVKAENSVAAKSDGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSGPTPGPSPLFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEAAGAYAPLTADGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAPQSVAEARGAGPPAGIHWYSQLLYHIGTWLLDSETLHPLGMAVKSS

Target ORF information:

RefSeq Version	NM_017221.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sonic hedgehog signaling molecule (Shh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017221.1

ORF Insert Sequence:

ATGCTGCTGC TGCTGGCCAG ATGTTTTCTG GTGGCCCTTG CTTCCTCGCT GCTGGTGTGC 
CCCGGACTGG CCTGTGGGCC CGGCAGGGGG TTTGGAAAGA GGCAGCACCC CAAAAAGCTG 
ACCCCTTTAG CCTACAAGCA GTTTATCCCC AACGTAGCCG AGAAGACCCT AGGGGCCAGC 
GGCCGATATG AAGGGAAGAT CACAAGAAAC TCCGAACGAT TTAAGGAACT CACCCCCAAT 
TACAACCCCG ACATCATATT TAAGGATGAG GAAAACACTG GAGCAGACCG GCTGATGACT 
CAGAGGTGCA AAGACAAGTT AAATGCCTTG GCCATCTCCG TGATGAACCA GTGGCCTGGA 
GTGAAGCTTC GAGTGACTGA GGGCTGGGAT GAGGACGGCC ATCATTCAGA GGAGTCTCTA 
CACTATGAGG GTCGAGCAGT GGACATCACC ACGTCTGACA GGGACCGCAG CAAGTATGGC 
ATGCTGGCTC GCCTGGCTGT GGAGGCTGGA TTCGACTGGG TCTACTATGA ATCCAAAGCT 
CGCATCCACT GCTCTGTGAA AGCAGAGAAC TCCGTGGCGG CCAAATCTGA CGGCTGCTTC 
CCGGGATCAG CCACAGTGCA CCTGGAGCAG GGTGGCACCA AGTTAGTGAA GGATCTAAGT 
CCCGGGGACC GCGTGCTGGC GGCTGACGAC CAGGGCCGGC TGCTGTACAG CGACTTCCTC 
ACCTTCCTGG ACCGCGACGA AGGTGCCAAG AAGGTCTTCT ACGTGATCGA GACGCGGGAG 
CCGCGGGAGC GTCTGCTGCT CACTGCCGCG CACCTGCTCT TCGTGGCGCC GCACAACGAC 
TCCGGGCCCA CTCCGGGACC GAGCCCACTC TTCGCCAGCC GCGTGCGTCC GGGGCAGCGC 
GTGTACGTGG TGGCTGAACG CGGCGGGGAC CGCCGGCTGC TGCCCGCCGC GGTGCACAGC 
GTAACGCTAC GAGAGGAGGC GGCGGGTGCG TACGCGCCGC TCACGGCGGA CGGCACCATT 
CTCATCAACC GGGTGCTCGC CTCGTGCTAC GCAGTCATCG AGGAGCACAG CTGGGCACAC 
CGGGCCTTCG CGCCCTTCCG CCTGGCGCAC GCGCTGCTGG CCGCGCTGGC ACCCGCCCGC 
ACGGACGGCG GGGGCGGGGG CAGCATCCCT GCCCCGCAAT CTGTAGCGGA AGCGAGGGGC 
GCAGGGCCGC CTGCGGGCAT CCACTGGTAC TCGCAGCTGC TGTACCACAT TGGCACCTGG 
CTGTTGGACA GCGAGACCCT GCATCCCTTG GGAATGGCAG TCAAGTCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CGRP attenuates hyperoxia-induced oxidative stress-related injury to alveolar epithelial type?II cells via the activation of the Sonic hedgehog pathway.
International journal of molecular medicine40(1)209-216(2017 Jul)
Dang HX,Li J,Liu C,Fu Y,Zhou F,Tang L,Li L,Xu F

High frequency stimulation induces sonic hedgehog release from hippocampal neurons.
Scientific reports743865(2017 Mar)
Su Y,Yuan Y,Feng S,Ma S,Wang Y

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Shh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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