Ptbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptbp1
Entrez Gene ID	29497
Full Name	polypyrimidine tract binding protein 1
Synonyms	PTB1,Ptb,Pybp,Pybp1,Pybp2
General protein information	Preferred Names polypyrimidine tract binding protein 1 Names polypyrimidine tract-binding protein 1 heterogeneous nuclear ribonucleoprotein I hnRNP I pyrimidine binding protein 1 pyrimidine binding protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	DNA binding protein; involved in the negative regulation of alternative splicing [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271057.1	NP_001257986.1	polypyrimidine tract-binding protein 1 isoform a
NM_022516.5	NP_071961.2	polypyrimidine tract-binding protein 1 isoform b

Pathways from BioSystems

Homologies

	PTBP1	XP_001092088.2
Danio rerio (zebrafish)	ptbp1b	NP_001116126.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003945	XP_318405.4
Arabidopsis thaliana (thale cress)	PTB3	NP_001077673.1
Xenopus tropicalis (tropical clawed frog)	ptbp1	NP_001011140.1
Homo sapiens (human)	PTBP1	NP_002810.1
Canis lupus familiaris (dog)	PTBP1	XP_542215.2
Caenorhabditis elegans (roundworm)	ptb-1	NP_741041.1
Drosophila melanogaster (fruit fly)	heph	NP_788776.2
Oryza sativa (rice)	Os05g0437300	NP_001055652.1
Pan troglodytes (chimpanzee)	PTBP1	XP_001172084.2
Bos taurus (cattle)	PTBP1	NP_776867.1
Mus musculus (house mouse)	Ptbp1	NP_001070831.1
Rattus norvegicus (Norway rat)	Ptbp1	NP_001257986.1
Gallus gallus (chicken)	PTBP1	NP_001026106.1
Danio rerio (zebrafish)	ptbp1a	NP_001018313.1
Oryza sativa (rice)	Os01g0867800	NP_001044916.1

Gene Ontology
Bibliography

Related articles in PubMed

An evolutionarily conserved long noncoding RNA TUNA controls pluripotency and neural lineage commitment.
Lin N, Chang KY, Li Z, Gates K, Rana ZA, Dang J, Zhang D, Han T, Yang CS, Cunningham TJ, Head SR, Duester G, Dong PD, Rana TM
Molecular cell53(6)1005-19(2014 Mar)

MicroRNA-124 suppresses the transactivation of nuclear factor of activated T cells by targeting multiple genes and inhibits the proliferation of pulmonary artery smooth muscle cells.
Kang K, Peng X, Zhang X, Wang Y, Zhang L, Gao L, Weng T, Zhang H, Ramchandran R, Raj JU, Gou D, Liu L
The Journal of biological chemistry288(35)25414-27(2013 Aug)

A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing.
Zheng S, Damoiseaux R, Chen L, Black DL
Genome research23(6)998-1007(2013 Jun)

MBNL1 and PTB cooperate to repress splicing of Tpm1 exon 3.
Gooding C, Edge C, Lorenz M, Coelho MB, Winters M, Kaminski CF, Cherny D, Eperon IC, Smith CW
Nucleic acids research41(9)4765-82(2013 May)

Alternative splicing: a pivotal step between eukaryotic transcription and translation.
Kornblihtt AR, Schor IE, All? M, Dujardin G, Petrillo E, Mu?oz MJ
Nature reviews. Molecular cell biology14(3)153-65(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that PTB and hnRNP L are positive regulators of Cat-1 mRNA translation via the IRES under stress conditions that cause a global decrease of protein synthesis.
Title: The hnRNA-binding proteins hnRNP L and PTB are required for efficient translation of the Cat-1 arginine/lysine transporter mRNA during amino acid starvation.

PTB1 is a common downstream target of glucose and GLP-1 for the posttranscriptional upregulation of granule biogenesis in beta cells.
Title: cAMP-dependent phosphorylation of PTB1 promotes the expression of insulin secretory granule proteins in beta cells.

analysis of the splicing repressor domain in polypyrimidine tract-binding protein
Title: A splicing repressor domain in polypyrimidine tract-binding protein.

Beta-cell stimulation induces the nucleocytoplasmic translocation of polypyrimidine tract-binding protein (PTB). Activated cytosolic PTB binds and stabilizes mRNAs encoding proteins of secretory granules, thus increasing their translation.
Title: Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis.

identification and characterization of a fourth rodent PTB paralog that is expressed at high levels in a number of smooth muscle tissues, localized to the nucleus, bound to RNA, and able to regulate alternative splicing
Title: A novel polypyrimidine tract-binding protein paralog expressed in smooth muscle cells.

The following Ptbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10121	NM_001271057.1 Latest version!	Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa05818	NM_022516.5 Latest version!	Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptbp1 ( NM_001271057.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257986.1 Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa10121
Clone ID Related Accession (Same CDS sequence)	NM_001271057.1
Accession Version	NM_001271057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	polypyrimidine tract-binding protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB772933.1 and BC061858.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061858.1, X74565.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACGGCA TCGTCCCAGA CATAGCAGTC GGTACAAAGC GGGGATCCGA CGAGCTCTTC 
TCCACGTGTG TCAGCAACGG CCCCTTCATC ATGAGCAGCT CGGCCTCAGC AGCCAACGGA 
AATGATAGCA AGAAGTTCAA AGGTGATAAC AGGAGCACAG GAGTTCCTTC CAGAGTCATC 
CATGTCCGAA AGCTACCTAG TGATGTCACT GAGGGCGAGG TCATCTCCCT GGGGCTGCCC 
TTTGGGAAAG TTACCAACCT CCTTATGCTG AAGGGGAAGA ACCAGGCCTT CATTGAAATG 
AGCACAGAGG AGGCTGCCAA CACTATGGTT AACTACTACA CATCGGTGGC GCCAGTGCTT 
CGTGGACAGC CCATCTACAT CCAGTTCTCC AACCACAAGG AGCTCAAAAC CGACAGCTCG 
CCCAACCAGG CACGTGCCCA GGCAGCCCTG CAGGCTGTGA ACTCCGTACA GTCAGGAAAC 
CTGGCCTTGG CAGCCTCTGC TGCTGCCGTG GATGCAGGAA TGGCAATGGC AGGCCAGAGC 
CCAGTGCTCA GGATCATTGT GGAGAACCTT TTCTACCCAG TGACACTGGA CGTGCTGCAC 
CAGATCTTCT CGAAGTTTGG CACAGTCCTG AAGATCATCA CGTTCACCAA GAACAACCAG 
TTCCAGGCGC TGCTGCAGTA TGCTGACCCT GTGAGCGCCC AGCATGCCAA GCTGTCCCTG 
GATGGCCAGA ACATCTACAA TGCCTGCTGC ACGCTGCGCA TCGACTTCTC CAAGCTCACC 
AGTCTCAACG TCAAATACAA CAACGACAAG AGCCGAGACT ACACACGCCC TGACCTGCCC 
TCTGGAGACA GCCAGCCATC ACTGGATCAG ACCATGGCAG CAGCCTTTGG TGCGCCCGGC 
ATAATGTCAG CCTCTCCGTA TGCAGGAGCC GGGTTCCCTC CCACCTTTGC CATCCCTCAG 
GCCGCAGGCC TCTCTGTTCC TAATGTCCAT GGAGCCCTGG CACCCCTGGC CATACCCTCT 
GCTGCTGCTG CTGCTGCTGC AGCCGGCCGC ATTGCCATCC CAGGGCTGGC AGGCGCCGGG 
AATTCCGTCC TTTTGGTCAG CAACTTGAAC CCTGAGAGAG TCACACCCCA AAGCCTCTTT 
ATTCTCTTCG GCGTCTACGG TGATGTGCAG CGGGTGAAGA TCCTGTTCAA CAAGAAGGAG 
AACGCACTTG TGCAGATGGC AGACGGCAGC CAGGCCCAGC TGGCCATGAG CCACCTGAAC 
GGGCACAAGC TGCATGGGAA GTCAGTGCGC ATCACCCTGT CAAAGCACCA GAGTGTGCAG 
CTGCCTCGTG AGGGACAGGA GGACCAGGGC CTGACCAAGG ACTATGGCAG CTCACCGCTG 
CACCGCTTCA AGAAACCTGG CTCCAAGAAC TTTCAGAACA TCTTTCCTCC CTCAGCTACC 
CTGCACCTCT CCAACATCCC GCCCTCTGTG TCAGAGGATG ACCTCAAGAG CCTCTTCTCC 
AGCAATGGCG GTGTGGTCAA AGGCTTCAAG TTCTTCCAGA AGGACCGCAA GATGGCACTG 
ATCCAGATGG GCTCTGTGGA GGAGGCAGTG CAGGCACTAA TTGAGCTGCA CAACCATGAC 
CTGGGCGAGA ACCACCACCT GCGAGTGTCC TTTTCCAAGT CCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257986.1
CDS	65..1735
Translation	MDGIVPDIAVGTKRGSDELFSTCVSNGPFIMSSSASAANGNDSKKFKGDNRSTGVPSRVIHVRKLPSDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMSTEEAANTMVNYYTSVAPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGAPGIMSASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKKENALVQMADGSQAQLAMSHLNGHKLHGKSVRITLSKHQSVQLPREGQEDQGLTKDYGSSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEDDLKSLFSSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIELHNHDLGENHHLRVSFSKSTI

Target ORF information:

RefSeq Version	NM_001271057.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271057.1

ORF Insert Sequence:

ATGGACGGCA TCGTCCCAGA CATAGCAGTC GGTACAAAGC GGGGATCCGA CGAGCTCTTC 
TCCACGTGTG TCAGCAACGG CCCCTTCATC ATGAGCAGCT CGGCCTCAGC AGCCAACGGA 
AATGATAGCA AGAAGTTCAA AGGTGATAAC AGGAGCACAG GAGTTCCTTC CAGAGTCATC 
CATGTCCGAA AGCTACCTAG TGATGTCACT GAGGGCGAGG TCATCTCCCT GGGGCTGCCC 
TTTGGGAAAG TTACCAACCT CCTTATGCTG AAGGGGAAGA ACCAGGCCTT CATTGAAATG 
AGCACAGAGG AGGCTGCCAA CACTATGGTT AACTACTACA CATCGGTGGC GCCAGTGCTT 
CGTGGACAGC CCATCTACAT CCAGTTCTCC AACCACAAGG AGCTCAAAAC CGACAGCTCG 
CCCAACCAGG CACGTGCCCA GGCAGCCCTG CAGGCTGTGA ACTCCGTACA GTCAGGAAAC 
CTGGCCTTGG CAGCCTCTGC TGCTGCCGTG GATGCAGGAA TGGCAATGGC AGGCCAGAGC 
CCAGTGCTCA GGATCATTGT GGAGAACCTT TTCTACCCAG TGACACTGGA CGTGCTGCAC 
CAGATCTTCT CGAAGTTTGG CACAGTCCTG AAGATCATCA CGTTCACCAA GAACAACCAG 
TTCCAGGCGC TGCTGCAGTA TGCTGACCCT GTGAGCGCCC AGCATGCCAA GCTGTCCCTG 
GATGGCCAGA ACATCTACAA TGCCTGCTGC ACGCTGCGCA TCGACTTCTC CAAGCTCACC 
AGTCTCAACG TCAAATACAA CAACGACAAG AGCCGAGACT ACACACGCCC TGACCTGCCC 
TCTGGAGACA GCCAGCCATC ACTGGATCAG ACCATGGCAG CAGCCTTTGG TGCGCCCGGC 
ATAATGTCAG CCTCTCCGTA TGCAGGAGCC GGGTTCCCTC CCACCTTTGC CATCCCTCAG 
GCCGCAGGCC TCTCTGTTCC TAATGTCCAT GGAGCCCTGG CACCCCTGGC CATACCCTCT 
GCTGCTGCTG CTGCTGCTGC AGCCGGCCGC ATTGCCATCC CAGGGCTGGC AGGCGCCGGG 
AATTCCGTCC TTTTGGTCAG CAACTTGAAC CCTGAGAGAG TCACACCCCA AAGCCTCTTT 
ATTCTCTTCG GCGTCTACGG TGATGTGCAG CGGGTGAAGA TCCTGTTCAA CAAGAAGGAG 
AACGCACTTG TGCAGATGGC AGACGGCAGC CAGGCCCAGC TGGCCATGAG CCACCTGAAC 
GGGCACAAGC TGCATGGGAA GTCAGTGCGC ATCACCCTGT CAAAGCACCA GAGTGTGCAG 
CTGCCTCGTG AGGGACAGGA GGACCAGGGC CTGACCAAGG ACTATGGCAG CTCACCGCTG 
CACCGCTTCA AGAAACCTGG CTCCAAGAAC TTTCAGAACA TCTTTCCTCC CTCAGCTACC 
CTGCACCTCT CCAACATCCC GCCCTCTGTG TCAGAGGATG ACCTCAAGAG CCTCTTCTCC 
AGCAATGGCG GTGTGGTCAA AGGCTTCAAG TTCTTCCAGA AGGACCGCAA GATGGCACTG 
ATCCAGATGG GCTCTGTGGA GGAGGCAGTG CAGGCACTAA TTGAGCTGCA CAACCATGAC 
CTGGGCGAGA ACCACCACCT GCGAGTGTCC TTTTCCAAGT CCACCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptbp1 ( NM_022516.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071961.2 Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa05818
Clone ID Related Accession (Same CDS sequence)	NM_022516.5
Accession Version	NM_022516.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Rattus norvegicus(Norway rat)
Product	polypyrimidine tract-binding protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB772933.1, FN803753.1, CK365398.1 and BC061858.1. On Aug 31, 2012 this sequence version replaced NM_022516.4. Transcript Variant: This variant (2) lacks a coding exon compared to variant 1. The resulting protein (isoform b) is shorter but has the same N- and C-termini compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X60789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACGGCA TCGTCCCAGA CATAGCAGTC GGTACAAAGC GGGGATCCGA CGAGCTCTTC 
TCCACGTGTG TCAGCAACGG CCCCTTCATC ATGAGCAGCT CGGCCTCAGC AGCCAACGGA 
AATGATAGCA AGAAGTTCAA AGGTGATAAC AGGAGCACAG GAGTTCCTTC CAGAGTCATC 
CATGTCCGAA AGCTACCTAG TGATGTCACT GAGGGCGAGG TCATCTCCCT GGGGCTGCCC 
TTTGGGAAAG TTACCAACCT CCTTATGCTG AAGGGGAAGA ACCAGGCCTT CATTGAAATG 
AGCACAGAGG AGGCTGCCAA CACTATGGTT AACTACTACA CATCGGTGGC GCCAGTGCTT 
CGTGGACAGC CCATCTACAT CCAGTTCTCC AACCACAAGG AGCTCAAAAC CGACAGCTCG 
CCCAACCAGG CACGTGCCCA GGCAGCCCTG CAGGCTGTGA ACTCCGTACA GTCAGGAAAC 
CTGGCCTTGG CAGCCTCTGC TGCTGCCGTG GATGCAGGAA TGGCAATGGC AGGCCAGAGC 
CCAGTGCTCA GGATCATTGT GGAGAACCTT TTCTACCCAG TGACACTGGA CGTGCTGCAC 
CAGATCTTCT CGAAGTTTGG CACAGTCCTG AAGATCATCA CGTTCACCAA GAACAACCAG 
TTCCAGGCGC TGCTGCAGTA TGCTGACCCT GTGAGCGCCC AGCATGCCAA GCTGTCCCTG 
GATGGCCAGA ACATCTACAA TGCCTGCTGC ACGCTGCGCA TCGACTTCTC CAAGCTCACC 
AGTCTCAACG TCAAATACAA CAACGACAAG AGCCGAGACT ACACACGCCC TGACCTGCCC 
TCTGGAGACA GCCAGCCATC ACTGGATCAG ACCATGGCAG CAGCCTTTGG CCTCTCTGTT 
CCTAATGTCC ATGGAGCCCT GGCACCCCTG GCCATACCCT CTGCTGCTGC TGCTGCTGCT 
GCAGCCGGCC GCATTGCCAT CCCAGGGCTG GCAGGCGCCG GGAATTCCGT CCTTTTGGTC 
AGCAACTTGA ACCCTGAGAG AGTCACACCC CAAAGCCTCT TTATTCTCTT CGGCGTCTAC 
GGTGATGTGC AGCGGGTGAA GATCCTGTTC AACAAGAAGG AGAACGCACT TGTGCAGATG 
GCAGACGGCA GCCAGGCCCA GCTGGCCATG AGCCACCTGA ACGGGCACAA GCTGCATGGG 
AAGTCAGTGC GCATCACCCT GTCAAAGCAC CAGAGTGTGC AGCTGCCTCG TGAGGGACAG 
GAGGACCAGG GCCTGACCAA GGACTATGGC AGCTCACCGC TGCACCGCTT CAAGAAACCT 
GGCTCCAAGA ACTTTCAGAA CATCTTTCCT CCCTCAGCTA CCCTGCACCT CTCCAACATC 
CCGCCCTCTG TGTCAGAGGA TGACCTCAAG AGCCTCTTCT CCAGCAATGG CGGTGTGGTC 
AAAGGCTTCA AGTTCTTCCA GAAGGACCGC AAGATGGCAC TGATCCAGAT GGGCTCTGTG 
GAGGAGGCAG TGCAGGCACT AATTGAGCTG CACAACCATG ACCTGGGCGA GAACCACCAC 
CTGCGAGTGT CCTTTTCCAA GTCCACCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071961.2
CDS	65..1657
Translation	MDGIVPDIAVGTKRGSDELFSTCVSNGPFIMSSSASAANGNDSKKFKGDNRSTGVPSRVIHVRKLPSDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMSTEEAANTMVNYYTSVAPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKKENALVQMADGSQAQLAMSHLNGHKLHGKSVRITLSKHQSVQLPREGQEDQGLTKDYGSSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEDDLKSLFSSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIELHNHDLGENHHLRVSFSKSTI

Target ORF information:

RefSeq Version	NM_022516.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022516.5

ORF Insert Sequence:

ATGGACGGCA TCGTCCCAGA CATAGCAGTC GGTACAAAGC GGGGATCCGA CGAGCTCTTC 
TCCACGTGTG TCAGCAACGG CCCCTTCATC ATGAGCAGCT CGGCCTCAGC AGCCAACGGA 
AATGATAGCA AGAAGTTCAA AGGTGATAAC AGGAGCACAG GAGTTCCTTC CAGAGTCATC 
CATGTCCGAA AGCTACCTAG TGATGTCACT GAGGGCGAGG TCATCTCCCT GGGGCTGCCC 
TTTGGGAAAG TTACCAACCT CCTTATGCTG AAGGGGAAGA ACCAGGCCTT CATTGAAATG 
AGCACAGAGG AGGCTGCCAA CACTATGGTT AACTACTACA CATCGGTGGC GCCAGTGCTT 
CGTGGACAGC CCATCTACAT CCAGTTCTCC AACCACAAGG AGCTCAAAAC CGACAGCTCG 
CCCAACCAGG CACGTGCCCA GGCAGCCCTG CAGGCTGTGA ACTCCGTACA GTCAGGAAAC 
CTGGCCTTGG CAGCCTCTGC TGCTGCCGTG GATGCAGGAA TGGCAATGGC AGGCCAGAGC 
CCAGTGCTCA GGATCATTGT GGAGAACCTT TTCTACCCAG TGACACTGGA CGTGCTGCAC 
CAGATCTTCT CGAAGTTTGG CACAGTCCTG AAGATCATCA CGTTCACCAA GAACAACCAG 
TTCCAGGCGC TGCTGCAGTA TGCTGACCCT GTGAGCGCCC AGCATGCCAA GCTGTCCCTG 
GATGGCCAGA ACATCTACAA TGCCTGCTGC ACGCTGCGCA TCGACTTCTC CAAGCTCACC 
AGTCTCAACG TCAAATACAA CAACGACAAG AGCCGAGACT ACACACGCCC TGACCTGCCC 
TCTGGAGACA GCCAGCCATC ACTGGATCAG ACCATGGCAG CAGCCTTTGG CCTCTCTGTT 
CCTAATGTCC ATGGAGCCCT GGCACCCCTG GCCATACCCT CTGCTGCTGC TGCTGCTGCT 
GCAGCCGGCC GCATTGCCAT CCCAGGGCTG GCAGGCGCCG GGAATTCCGT CCTTTTGGTC 
AGCAACTTGA ACCCTGAGAG AGTCACACCC CAAAGCCTCT TTATTCTCTT CGGCGTCTAC 
GGTGATGTGC AGCGGGTGAA GATCCTGTTC AACAAGAAGG AGAACGCACT TGTGCAGATG 
GCAGACGGCA GCCAGGCCCA GCTGGCCATG AGCCACCTGA ACGGGCACAA GCTGCATGGG 
AAGTCAGTGC GCATCACCCT GTCAAAGCAC CAGAGTGTGC AGCTGCCTCG TGAGGGACAG 
GAGGACCAGG GCCTGACCAA GGACTATGGC AGCTCACCGC TGCACCGCTT CAAGAAACCT 
GGCTCCAAGA ACTTTCAGAA CATCTTTCCT CCCTCAGCTA CCCTGCACCT CTCCAACATC 
CCGCCCTCTG TGTCAGAGGA TGACCTCAAG AGCCTCTTCT CCAGCAATGG CGGTGTGGTC 
AAAGGCTTCA AGTTCTTCCA GAAGGACCGC AAGATGGCAC TGATCCAGAT GGGCTCTGTG 
GAGGAGGCAG TGCAGGCACT AATTGAGCTG CACAACCATG ACCTGGGCGA GAACCACCAC 
CTGCGAGTGT CCTTTTCCAA GTCCACCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An evolutionarily conserved long noncoding RNA TUNA controls pluripotency and neural lineage commitment.
Molecular cell53(6)1005-19(2014 Mar)
Lin N,Chang KY,Li Z,Gates K,Rana ZA,Dang J,Zhang D,Han T,Yang CS,Cunningham TJ,Head SR,Duester G,Dong PD,Rana TM

MicroRNA-124 suppresses the transactivation of nuclear factor of activated T cells by targeting multiple genes and inhibits the proliferation of pulmonary artery smooth muscle cells.
The Journal of biological chemistry288(35)25414-27(2013 Aug)
Kang K,Peng X,Zhang X,Wang Y,Zhang L,Gao L,Weng T,Zhang H,Ramchandran R,Raj JU,Gou D,Liu L

A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing.
Genome research23(6)998-1007(2013 Jun)
Zheng S,Damoiseaux R,Chen L,Black DL

MBNL1 and PTB cooperate to repress splicing of Tpm1 exon 3.
Nucleic acids research41(9)4765-82(2013 May)
Gooding C,Edge C,Lorenz M,Coelho MB,Winters M,Kaminski CF,Cherny D,Eperon IC,Smith CW

Alternative splicing: a pivotal step between eukaryotic transcription and translation.
Nature reviews. Molecular cell biology14(3)153-65(2013 Mar)
Kornblihtt AR,Schor IE,All? M,Dujardin G,Petrillo E,Mu?oz MJ

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Ptbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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