Tars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tars
Entrez Gene ID	294810
Full Name	threonyl-tRNA synthetase
General protein information	Preferred Names threonyl-tRNA synthetase Names threonine--tRNA ligase, cytoplasmic thrRS threonyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001006976.1	NP_001006977.1	threonine--tRNA ligase 1, cytoplasmic
XM_006232068.3	XP_006232130.1	threonine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tars	NP_001116258.1
Drosophila melanogaster (fruit fly)	Aats-thr	NP_524839.3
Caenorhabditis elegans (roundworm)	tars-1	NP_001022033.1
Magnaporthe oryzae (rice blast fungus)	MGG_03551	XP_003716364.1
Arabidopsis thaliana (thale cress)	AT5G26830	NP_198035.2
Rattus norvegicus (Norway rat)	Tars	NP_001006977.1
Gallus gallus (chicken)	TARS	NP_001026618.1
Saccharomyces cerevisiae (baker's yeast)	THS1	NP_116578.3
Schizosaccharomyces pombe (fission yeast)	trs1	NP_596364.1
Canis lupus familiaris (dog)	TARS	XP_536509.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008929	XP_319682.4
Eremothecium gossypii	AGOS_ACL039W	NP_983365.1
Neurospora crassa	NCU02380	XP_959801.1
Homo sapiens (human)	TARS	NP_001245367.1
Oryza sativa (rice)	Os08g0295300	NP_001061475.1
Xenopus tropicalis (tropical clawed frog)	tars	NP_001001219.1
Pan troglodytes (chimpanzee)	TARS	XP_003310808.1
Bos taurus (cattle)	TARS	NP_001029542.1
Mus musculus (house mouse)	Tars	NP_149065.2
Kluyveromyces lactis	KLLA0F05918g	XP_455348.1

Gene Ontology
Bibliography

Related articles in PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
Kim JY, Kang YS, Lee JW, Kim HJ, Ahn YH, Park H, Ko YG, Kim S
Proceedings of the National Academy of Sciences of the United States of America99(12)7912-6(2002 Jun)

Threonyl-tRNA synthetase.
Freist W, Gauss DH
Biological chemistry Hoppe-Seyler376(4)213-24(1995 Apr)

Presence of role of the 5SrRNA-L5 protein complex (5SRNP) in the threonyl- and histidyl-tRNA synthetase complex in rat liver cytosol.
Ogata K, Kurahashi A, Nishiyama C, Terao K
Biochimica et biophysica acta1218(3)388-400(1994 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10685	NM_001006976.1 Latest version!	Rattus norvegicus threonyl-tRNA synthetase 1 (Tars1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa24485	XM_006232068.3 Latest version!	Rattus norvegicus threonyl-tRNA synthetase (Tars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tars ( NM_001006976.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001006977.1 Rattus norvegicus threonyl-tRNA synthetase 1 (Tars1), mRNA.

Price & Availability↑

CloneID	ORa10685
Clone ID Related Accession (Same CDS sequence)	NM_001006976.1
Accession Version	NM_001006976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Rattus norvegicus(Norway rat)
Product	threonine--tRNA ligase 1, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083914.1. On Nov 22, 2004 this sequence version replaced XM_215506.2. ##Evidence-Data-START## Transcript exon combination :: BC083914.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGTTGAAT 
CCCTGGCCGG AGTACATTAA CACACGCCTT GATATGTACC ACAAGCTCAA AGCAGAGCAT 
GATTCCATCC TGGCTGAAAA GGCAGCAAAA GACAGCAAGC CGATTAAAGT CACCCTGCCC 
GACGGCAAGC AAGTGGATGC TGAGTCCTGG AAAACCACGC CGTATCAGAT TGCATGTGGC 
ATTAGCCAGG GCCTGGCTGA CAACACCGTT GTTGCTAAGG TGAACAAGGT TGTTTGGGAC 
CTGGACCGCC CGCTGGAGAC AGACTGCACT CTGGAGCTGC TCAAGTTTGA GGACGAGGAA 
GCACAGGCAG TATATTGGCA CTCTAGTGCC CACATAATGG GTGAGGCCAT GGAAAGAGTC 
TACGGTGGAT GCTTATGTTA CGGCCCTCCA ATAGAAAACG GCTTCTATTA TGATATGTAC 
CTTGAAGAAG GGGGTGTGTC CAGCAATGAT TTCTCTTCCC TGGAAACTTT GTGTAAGAAA 
ATCATTAAAG AGAAACAAAC TTTTGAAAGA TTGGAAGTTA AAAAGGAAAC ATTACTAGAA 
ATGTTCAAGT ACAACAAGTT CAAGTGCCGG ATATTGAATG AGAAAGTGAA TACTCCGACC 
ACGACTGTCT ATAGATGTGG CCCCTTGATA GATCTCTGCC GTGGTCCTCA CGTCCGACAC 
ACTGGCAAGA TTAAGACTCT GAAAATACAC AAGAATTCCT CCACGTACTG GGAAGGCAAA 
GCGGACATGG AAACGCTCCA GAGGATTTAT GGGATTTCAT TCCCCGACCC CAAACTACTG 
AAAGAGTGGG AGAAGTTCCA AGAGGAAGCC AAGAACCGAG ACCATAGGAA GATTGGGCGG 
GACCAAGAAC TATATTTCTT CCACGAACTC AGCCCTGGAA GTTGCTTTTT CCTGCCAAAA 
GGAGCCTACA TTTATAATAC ACTGATGGAA TTCATCAGGA GTGAATATAG GAAAAGAGGG 
TTCCAGGAGG TCGTCACCCC CAACATCTTC AATAGCCGGC TCTGGATGAC CTCTGGCCAC 
TGGCAGCACT ACAGTGAAAA CATGTTCTCC TTTGAGGTGG AGAAGGAGCA GTTTGCCCTC 
AAGCCCATGA ACTGCCCAGG ACACTGCCTG ATGTTCGATC ATCGGCCGAG GTCCTGGCGA 
GAGCTGCCCC TGCGGTTAGC TGATTTTGGA GTGCTTCACA GGAACGAGCT CTCAGGGGCT 
CTCACTGGAC TCACACGGGT CCGAAGATTC CAGCAGGATG ACGCACACAT ATTCTGTGCC 
ATGGAGCAGA TTGAAGATGA GATCAAAGGT TGTTTGGATT TTCTTCGCAC GGTATATAGT 
GTCTTTGGAT TTTCATTTAA ACTGAATCTT TCTACTCGCC CAGAAAAATT CCTGGGAGAT 
ATTGAAATAT GGAACCAAGC TGAGAAACAA CTTGAAAACA GTTTGAACGA ATTTGGTGAG 
AAGTGGGAGC TAAATCCTGG CGATGGAGCC TTCTATGGTC CAAAGATTGA TATACAGATA 
AAGGACGCTA TTGGCCGCTA CCACCAGTGT GCGACCATCC AGCTGGACTT TCAGTTGCCC 
ATCAGGTTTA ACCTCACTTA CGTCAGCCAT GATGGCGACG ATAAGAAGAG GCCAGTGATC 
GTTCACCGAG CCATCCTGGG GTCAGTGGAG AGAATGATTG CCATCCTCAC GGAAAACTAT 
GGGGGCAAAT GGCCTTTCTG GCTCTCTCCT CGCCAGGTGA TGGTGGTCCC AGTGGGACCG 
ACCTGTGATG AATATGCCCA AAAGGTACGG CAAGAATTCC ACGATGCTAA GTTCATGGTG 
GACATTGACC TGGATCCAGG CTGTACCTTG AATAAGAAGA TCAGAAATGC ACAGTTGGCA 
CAGTATAACT TCATCCTTGT TGTTGGTGAA AAAGAGAAAG CCAGTGGCAC TGTGAACATC 
CGCACGAGAG ACAACAAGGT CCACGGAGAG CGGACTGTGG GGGAAACAGT GGAGCGGCTG 
CAGCAGCTCA AGCAGTTGCG CAGTAAGCAG GCAGAGGAGG AGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006977.1
CDS	51..2138
Translation	MSEEKASSPSGKMDGEKPLNPWPEYINTRLDMYHKLKAEHDSILAEKAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQEFHDAKFMVDIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTVGETVERLQQLKQLRSKQAEEEF

Target ORF information:

RefSeq Version	NM_001006976.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus threonyl-tRNA synthetase 1 (Tars1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006976.1

ORF Insert Sequence:

ATGTCGGAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGTTGAAT 
CCCTGGCCGG AGTACATTAA CACACGCCTT GATATGTACC ACAAGCTCAA AGCAGAGCAT 
GATTCCATCC TGGCTGAAAA GGCAGCAAAA GACAGCAAGC CGATTAAAGT CACCCTGCCC 
GACGGCAAGC AAGTGGATGC TGAGTCCTGG AAAACCACGC CGTATCAGAT TGCATGTGGC 
ATTAGCCAGG GCCTGGCTGA CAACACCGTT GTTGCTAAGG TGAACAAGGT TGTTTGGGAC 
CTGGACCGCC CGCTGGAGAC AGACTGCACT CTGGAGCTGC TCAAGTTTGA GGACGAGGAA 
GCACAGGCAG TATATTGGCA CTCTAGTGCC CACATAATGG GTGAGGCCAT GGAAAGAGTC 
TACGGTGGAT GCTTATGTTA CGGCCCTCCA ATAGAAAACG GCTTCTATTA TGATATGTAC 
CTTGAAGAAG GGGGTGTGTC CAGCAATGAT TTCTCTTCCC TGGAAACTTT GTGTAAGAAA 
ATCATTAAAG AGAAACAAAC TTTTGAAAGA TTGGAAGTTA AAAAGGAAAC ATTACTAGAA 
ATGTTCAAGT ACAACAAGTT CAAGTGCCGG ATATTGAATG AGAAAGTGAA TACTCCGACC 
ACGACTGTCT ATAGATGTGG CCCCTTGATA GATCTCTGCC GTGGTCCTCA CGTCCGACAC 
ACTGGCAAGA TTAAGACTCT GAAAATACAC AAGAATTCCT CCACGTACTG GGAAGGCAAA 
GCGGACATGG AAACGCTCCA GAGGATTTAT GGGATTTCAT TCCCCGACCC CAAACTACTG 
AAAGAGTGGG AGAAGTTCCA AGAGGAAGCC AAGAACCGAG ACCATAGGAA GATTGGGCGG 
GACCAAGAAC TATATTTCTT CCACGAACTC AGCCCTGGAA GTTGCTTTTT CCTGCCAAAA 
GGAGCCTACA TTTATAATAC ACTGATGGAA TTCATCAGGA GTGAATATAG GAAAAGAGGG 
TTCCAGGAGG TCGTCACCCC CAACATCTTC AATAGCCGGC TCTGGATGAC CTCTGGCCAC 
TGGCAGCACT ACAGTGAAAA CATGTTCTCC TTTGAGGTGG AGAAGGAGCA GTTTGCCCTC 
AAGCCCATGA ACTGCCCAGG ACACTGCCTG ATGTTCGATC ATCGGCCGAG GTCCTGGCGA 
GAGCTGCCCC TGCGGTTAGC TGATTTTGGA GTGCTTCACA GGAACGAGCT CTCAGGGGCT 
CTCACTGGAC TCACACGGGT CCGAAGATTC CAGCAGGATG ACGCACACAT ATTCTGTGCC 
ATGGAGCAGA TTGAAGATGA GATCAAAGGT TGTTTGGATT TTCTTCGCAC GGTATATAGT 
GTCTTTGGAT TTTCATTTAA ACTGAATCTT TCTACTCGCC CAGAAAAATT CCTGGGAGAT 
ATTGAAATAT GGAACCAAGC TGAGAAACAA CTTGAAAACA GTTTGAACGA ATTTGGTGAG 
AAGTGGGAGC TAAATCCTGG CGATGGAGCC TTCTATGGTC CAAAGATTGA TATACAGATA 
AAGGACGCTA TTGGCCGCTA CCACCAGTGT GCGACCATCC AGCTGGACTT TCAGTTGCCC 
ATCAGGTTTA ACCTCACTTA CGTCAGCCAT GATGGCGACG ATAAGAAGAG GCCAGTGATC 
GTTCACCGAG CCATCCTGGG GTCAGTGGAG AGAATGATTG CCATCCTCAC GGAAAACTAT 
GGGGGCAAAT GGCCTTTCTG GCTCTCTCCT CGCCAGGTGA TGGTGGTCCC AGTGGGACCG 
ACCTGTGATG AATATGCCCA AAAGGTACGG CAAGAATTCC ACGATGCTAA GTTCATGGTG 
GACATTGACC TGGATCCAGG CTGTACCTTG AATAAGAAGA TCAGAAATGC ACAGTTGGCA 
CAGTATAACT TCATCCTTGT TGTTGGTGAA AAAGAGAAAG CCAGTGGCAC TGTGAACATC 
CGCACGAGAG ACAACAAGGT CCACGGAGAG CGGACTGTGG GGGAAACAGT GGAGCGGCTG 
CAGCAGCTCA AGCAGTTGCG CAGTAAGCAG GCAGAGGAGG AGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tars ( XM_006232068.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232130.1 Rattus norvegicus threonyl-tRNA synthetase (Tars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24485
Clone ID Related Accession (Same CDS sequence)	XM_006232068.3
Accession Version	XM_006232068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	threonine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGGTGGAT 
GCCAGCGAGG AGAAGCGAAA AGAAGGAGGC AAGAAGAGGA ACAAAGATGG AGGTGGAGAC 
GGAGGTCGAG CAGAGTTGAA TCCCTGGCCG GAGTACATTA ACACACGCCT TGATATGTAC 
CACAAGCTCA AAGCAGAGCA TGATTCCATC CTGGCTGAAA AGGCAGCAAA AGACAGCAAG 
CCGATTAAAG TCACCCTGCC CGACGGCAAG CAAGTGGATG CTGAGTCCTG GAAAACCACG 
CCGTATCAGA TTGCATGTGG CATTAGCCAG GGCCTGGCTG ACAACACCGT TGTTGCTAAG 
GTGAACAAGG TTGTTTGGGA CCTGGACCGC CCGCTGGAGA CAGACTGCAC TCTGGAGCTG 
CTCAAGTTTG AGGACGAGGA AGCACAGGCA GTATATTGGC ACTCTAGTGC CCACATAATG 
GGTGAGGCCA TGGAAAGAGT CTACGGTGGA TGCTTATGTT ACGGCCCTCC AATAGAAAAC 
GGCTTCTATT ATGATATGTA CCTTGAAGAA GGGGGTGTGT CCAGCAATGA TTTCTCTTCC 
CTGGAAACTT TGTGTAAGAA AATCATTAAA GAGAAACAAA CTTTTGAAAG ATTGGAAGTT 
AAAAAGGAAA CATTACTAGA AATGTTCAAG TACAACAAGT TCAAGTGCCG GATATTGAAT 
GAGAAAGTGA ATACTCCGAC CACGACTGTC TATAGATGTG GCCCCTTGAT AGATCTCTGC 
CGTGGTCCTC ACGTCCGACA CACTGGCAAG ATTAAGACTC TGAAAATACA CAAGAATTCC 
TCCACGTACT GGGAAGGCAA AGCGGACATG GAAACGCTCC AGAGGATTTA TGGGATTTCA 
TTCCCCGACC CCAAACTACT GAAAGAGTGG GAGAAGTTCC AAGAGGAAGC CAAGAACCGA 
GACCATAGGA AGATTGGGCG GGACCAAGAA CTATATTTCT TCCACGAACT CAGCCCTGGA 
AGTTGCTTTT TCCTGCCAAA AGGAGCCTAC ATTTATAATA CACTGATGGA ATTCATCAGG 
AGTGAATATA GGAAAAGAGG GTTCCAGGAG GTCGTCACCC CCAACATCTT CAATAGCCGG 
CTCTGGATGA CCTCTGGCCA CTGGCAGCAC TACAGTGAAA ACATGTTCTC CTTTGAGGTG 
GAGAAGGAGC AGTTTGCCCT CAAGCCCATG AACTGCCCAG GACACTGCCT GATGTTCGAT 
CATCGGCCGA GGTCCTGGCG AGAGCTGCCC CTGCGGTTAG CTGATTTTGG AGTGCTTCAC 
AGGAACGAGC TCTCAGGGGC TCTCACTGGA CTCACACGGG TCCGAAGATT CCAGCAGGAT 
GACGCACACA TATTCTGTGC CATGGAGCAG ATTGAAGATG AGATCAAAGG TTGTTTGGAT 
TTTCTTCGCA CGGTATATAG TGTCTTTGGA TTTTCATTTA AACTGAATCT TTCTACTCGC 
CCAGAAAAAT TCCTGGGAGA TATTGAAATA TGGAACCAAG CTGAGAAACA ACTTGAAAAC 
AGTTTGAACG AATTTGGTGA GAAGTGGGAG CTAAATCCTG GCGATGGAGC CTTCTATGGT 
CCAAAGATTG ATATACAGAT AAAGGACGCT ATTGGCCGCT ACCACCAGTG TGCGACCATC 
CAGCTGGACT TTCAGTTGCC CATCAGGTTT AACCTCACTT ACGTCAGCCA TGATGGCGAC 
GATAAGAAGA GGCCAGTGAT CGTTCACCGA GCCATCCTGG GGTCAGTGGA GAGAATGATT 
GCCATCCTCA CGGAAAACTA TGGGGGCAAA TGGCCTTTCT GGCTCTCTCC TCGCCAGGTG 
ATGGTGGTCC CAGTGGGACC GACCTGTGAT GAATATGCCC AAAAGGTACG GCAAGAATTC 
CACGATGCTA AGTTCATGGT GGACATTGAC CTGGATCCAG GCTGTACCTT GAATAAGAAG 
ATCAGAAATG CACAGTTGGC ACAGTATAAC TTCATCCTTG TTGTTGGTGA AAAAGAGAAA 
GCCAGTGGCA CTGTGAACAT CCGCACGAGA GACAACAAGG TCCACGGAGA GCGGACTGTG 
GGGGAAACAG TGGAGCGGCT GCAGCAGCTC AAGCAGTTGC GCAGTAAGCA GGCAGAGGAG 
GAGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232130.1
CDS	128..2296
Translation	MSEEKASSPSGKMDGEKPVDASEEKRKEGGKKRNKDGGGDGGRAELNPWPEYINTRLDMYHKLKAEHDSILAEKAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQEFHDAKFMVDIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTVGETVERLQQLKQLRSKQAEEEF

Target ORF information:

RefSeq Version	XM_006232068.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus threonyl-tRNA synthetase (Tars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232068.3

ORF Insert Sequence:

ATGTCGGAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGGTGGAT 
GCCAGCGAGG AGAAGCGAAA AGAAGGAGGC AAGAAGAGGA ACAAAGATGG AGGTGGAGAC 
GGAGGTCGAG CAGAGTTGAA TCCCTGGCCG GAGTACATTA ACACACGCCT TGATATGTAC 
CACAAGCTCA AAGCAGAGCA TGATTCCATC CTGGCTGAAA AGGCAGCAAA AGACAGCAAG 
CCGATTAAAG TCACCCTGCC CGACGGCAAG CAAGTGGATG CTGAGTCCTG GAAAACCACG 
CCGTATCAGA TTGCATGTGG CATTAGCCAG GGCCTGGCTG ACAACACCGT TGTTGCTAAG 
GTGAACAAGG TTGTTTGGGA CCTGGACCGC CCGCTGGAGA CAGACTGCAC TCTGGAGCTG 
CTCAAGTTTG AGGACGAGGA AGCACAGGCA GTATATTGGC ACTCTAGTGC CCACATAATG 
GGTGAGGCCA TGGAAAGAGT CTACGGTGGA TGCTTATGTT ACGGCCCTCC AATAGAAAAC 
GGCTTCTATT ATGATATGTA CCTTGAAGAA GGGGGTGTGT CCAGCAATGA TTTCTCTTCC 
CTGGAAACTT TGTGTAAGAA AATCATTAAA GAGAAACAAA CTTTTGAAAG ATTGGAAGTT 
AAAAAGGAAA CATTACTAGA AATGTTCAAG TACAACAAGT TCAAGTGCCG GATATTGAAT 
GAGAAAGTGA ATACTCCGAC CACGACTGTC TATAGATGTG GCCCCTTGAT AGATCTCTGC 
CGTGGTCCTC ACGTCCGACA CACTGGCAAG ATTAAGACTC TGAAAATACA CAAGAATTCC 
TCCACGTACT GGGAAGGCAA AGCGGACATG GAAACGCTCC AGAGGATTTA TGGGATTTCA 
TTCCCCGACC CCAAACTACT GAAAGAGTGG GAGAAGTTCC AAGAGGAAGC CAAGAACCGA 
GACCATAGGA AGATTGGGCG GGACCAAGAA CTATATTTCT TCCACGAACT CAGCCCTGGA 
AGTTGCTTTT TCCTGCCAAA AGGAGCCTAC ATTTATAATA CACTGATGGA ATTCATCAGG 
AGTGAATATA GGAAAAGAGG GTTCCAGGAG GTCGTCACCC CCAACATCTT CAATAGCCGG 
CTCTGGATGA CCTCTGGCCA CTGGCAGCAC TACAGTGAAA ACATGTTCTC CTTTGAGGTG 
GAGAAGGAGC AGTTTGCCCT CAAGCCCATG AACTGCCCAG GACACTGCCT GATGTTCGAT 
CATCGGCCGA GGTCCTGGCG AGAGCTGCCC CTGCGGTTAG CTGATTTTGG AGTGCTTCAC 
AGGAACGAGC TCTCAGGGGC TCTCACTGGA CTCACACGGG TCCGAAGATT CCAGCAGGAT 
GACGCACACA TATTCTGTGC CATGGAGCAG ATTGAAGATG AGATCAAAGG TTGTTTGGAT 
TTTCTTCGCA CGGTATATAG TGTCTTTGGA TTTTCATTTA AACTGAATCT TTCTACTCGC 
CCAGAAAAAT TCCTGGGAGA TATTGAAATA TGGAACCAAG CTGAGAAACA ACTTGAAAAC 
AGTTTGAACG AATTTGGTGA GAAGTGGGAG CTAAATCCTG GCGATGGAGC CTTCTATGGT 
CCAAAGATTG ATATACAGAT AAAGGACGCT ATTGGCCGCT ACCACCAGTG TGCGACCATC 
CAGCTGGACT TTCAGTTGCC CATCAGGTTT AACCTCACTT ACGTCAGCCA TGATGGCGAC 
GATAAGAAGA GGCCAGTGAT CGTTCACCGA GCCATCCTGG GGTCAGTGGA GAGAATGATT 
GCCATCCTCA CGGAAAACTA TGGGGGCAAA TGGCCTTTCT GGCTCTCTCC TCGCCAGGTG 
ATGGTGGTCC CAGTGGGACC GACCTGTGAT GAATATGCCC AAAAGGTACG GCAAGAATTC 
CACGATGCTA AGTTCATGGT GGACATTGAC CTGGATCCAG GCTGTACCTT GAATAAGAAG 
ATCAGAAATG CACAGTTGGC ACAGTATAAC TTCATCCTTG TTGTTGGTGA AAAAGAGAAA 
GCCAGTGGCA CTGTGAACAT CCGCACGAGA GACAACAAGG TCCACGGAGA GCGGACTGTG 
GGGGAAACAG TGGAGCGGCT GCAGCAGCTC AAGCAGTTGC GCAGTAAGCA GGCAGAGGAG 
GAGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
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Threonyl-tRNA synthetase.
Biological chemistry Hoppe-Seyler376(4)213-24(1995 Apr)
Freist W,Gauss DH

Presence of role of the 5SrRNA-L5 protein complex (5SRNP) in the threonyl- and histidyl-tRNA synthetase complex in rat liver cytosol.
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Tars cDNA ORF clone, Rattus norvegicus(Norway rat)

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