Wasf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wasf1
Entrez Gene ID	294568
Full Name	WAS protein family, member 1
General protein information	Preferred Names WAS protein family, member 1 Names wiskott-Aldrich syndrome protein family member 1 WASP family 1 WASP family protein member 1 protein WAVE-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256586.3	XP_006256648.1	wiskott-Aldrich syndrome protein family member 1 isoform X1
NM_001025114.1	NP_001020285.1	wiskott-Aldrich syndrome protein family member 1
XM_006256587.3	XP_006256649.1	wiskott-Aldrich syndrome protein family member 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WASF1	XP_005627764.1
Mus musculus (house mouse)	Wasf1	NP_114083.1
Rattus norvegicus (Norway rat)	Wasf1	NP_001020285.1
Pan troglodytes (chimpanzee)	WASF1	NP_001233409.1
Xenopus tropicalis (tropical clawed frog)	wasf1	NP_001096217.1
Gallus gallus (chicken)	WASF1	XP_004940365.1
Bos taurus (cattle)	WASF1	NP_001029189.2
Danio rerio (zebrafish)	si:dkeyp-88f5.5	XP_001335802.5
Homo sapiens (human)	WASF1	NP_001020105.1

Gene Ontology
Bibliography

Related articles in PubMed

Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth.
Xu C, Fu X, Zhu S, Liu JJ
Molecular biology of the cell27(21)3342-3356(2016 Nov)

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Burgers PP, van der Heyden MA, Kok B, Heck AJ, Scholten A
Biochemistry54(1)11-21(2015 Jan)

Structure and control of the actin regulatory WAVE complex.
Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK
Nature468(7323)533-8(2010 Nov)

Rapid signaling of estrogen to WAVE1 and moesin controls neuronal spine formation via the actin cytoskeleton.
Sanchez AM, Flamini MI, Fu XD, Mannella P, Giretti MS, Goglia L, Genazzani AR, Simoncini T
Molecular endocrinology (Baltimore, Md.)23(8)1193-202(2009 Aug)

WAVE1 controls neuronal activity-induced mitochondrial distribution in dendritic spines.
Sung JY, Engmann O, Teylan MA, Nairn AC, Greengard P, Kim Y
Proceedings of the National Academy of Sciences of the United States of America105(8)3112-6(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the control of actin polymerization and branching via moesin or WAVE1 is a key function of estrogen receptor alpha in neurons, which may be particularly relevant for the regulation of dendritic spines
Title: Rapid signaling of estrogen to WAVE1 and moesin controls neuronal spine formation via the actin cytoskeleton.

Together, these data demonstrate a role for WAVE1 in oligodendrocyte morphogenesis and myelination.
Title: WAVE1 is required for oligodendrocyte morphogenesis and normal CNS myelination.

The WAVE1 G-protein regulatory (GPR) peptide inhibited GTPgammaS binding to purified G-protein.A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1.
Title: Identification and characterization of a G-protein regulatory motif in WAVE1.

The following Wasf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wasf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07230	NM_001025114.1 Latest version!	Rattus norvegicus WASP family member 1 (Wasf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa07230	XM_006256586.3 Latest version!	Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa39547	XM_006256587.3 Latest version!	Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wasf1 ( NM_001025114.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020285.1 Rattus norvegicus WASP family member 1 (Wasf1), mRNA.

Price & Availability↑

CloneID	ORa07230
Clone ID Related Accession (Same CDS sequence)	XM_006256586.3 , NM_001025114.1
Accession Version	NM_001025114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	wiskott-Aldrich syndrome protein family member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091322.1. On Jul 1, 2005 this sequence version replaced XM_215432.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
CAGAAAAATC TAGACCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC ACATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCCGATCTC 
CTCCACAAGC ACATCGAAGT TGCTAACGGC CCAGCCTCTC ATTTTGAGAC AAGGCCGCAG 
ACATACGTGG ACCACATGGA TGGATCTTAC TCACTCTCTG CCTTGCCATT CAGTCAGATG 
AGTGAACTCC TAAGCAGAGC TGAGGAGAGG GTCTTAGTCA GACCCCATGA ACCACCTCCA 
CCTCCACCAA TGCATGCAGC AGGGGATGCA AGACCCACCC CCACCTGTGT CAGCTCTGCT 
GCGGGTTTGA TAGAGAATCG TCCCCAGTCC CCAGCTGCAG GCAGAACACC CGTGTTTGTG 
AGCCCCACGC CCCCACCTCC TCCACCACCT CTCCCGTCTG CCCTGTCCAC CTCCTCACTG 
AGAGCTTCCA TGACCTCAAC TCCTCCCCCA CCGGTGCCTC CCCCGCCTCC TCCTCCAGCC 
CCTGCCTTGC AGGCCCCAGC AGTGCCCCCG CCTCCAGCCC CTCTTCAGAT TGCCCCTGGT 
GTCCTTCACC CAGCTCCTCC TCCAATCGCT CCTCCTCTAG TACAGCCCTC TCCCCCGGTA 
GCCAGAGCCG CCCCAGTGTG TGAGACTGTA CCCGTTCATC CACTCCCACA AGGTGAAGTG 
CAGGGGCTGC CTCCGCCCCC GCCGCCGCCG CCTCTGCCTC CTCCAGGCAT TCGGCCATCA 
TCACCTGTCA CGGTCGCAGC TCTTGCGCAT CCTCCCTCTG GGCTCCACCC AACTCCATCT 
CCTGCCCCTG GTCCCCATGC TCCATTAATG CCTCCGTCCC CACCTTCACA AGTCCTGCCT 
GCCTCTGAGC CAAAGCGCCA TCCTTCCACC CTACCCGTGA TCAGTGACGC GAGGAGTGTG 
CTGCTGGAGG CCATACGGAA AGGCATTCAG CTTCGCAAAG TGGAAGAGCA GCGTGAACAG 
GAAGCAAAGC ATGAGCGGAT TGAAAACGAC GTGGCCACCA TCCTGTCACG CCGCATCGCT 
GTGGAGTACA GCGACTCGGA AGACGACTCG GAGTTCGATG AAGTAGACTG GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020285.1
CDS	72..1751
Translation	MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPPPPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

Target ORF information:

RefSeq Version	NM_001025114.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WASP family member 1 (Wasf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025114.1

ORF Insert Sequence:

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
CAGAAAAATC TAGACCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC ACATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCCGATCTC 
CTCCACAAGC ACATCGAAGT TGCTAACGGC CCAGCCTCTC ATTTTGAGAC AAGGCCGCAG 
ACATACGTGG ACCACATGGA TGGATCTTAC TCACTCTCTG CCTTGCCATT CAGTCAGATG 
AGTGAACTCC TAAGCAGAGC TGAGGAGAGG GTCTTAGTCA GACCCCATGA ACCACCTCCA 
CCTCCACCAA TGCATGCAGC AGGGGATGCA AGACCCACCC CCACCTGTGT CAGCTCTGCT 
GCGGGTTTGA TAGAGAATCG TCCCCAGTCC CCAGCTGCAG GCAGAACACC CGTGTTTGTG 
AGCCCCACGC CCCCACCTCC TCCACCACCT CTCCCGTCTG CCCTGTCCAC CTCCTCACTG 
AGAGCTTCCA TGACCTCAAC TCCTCCCCCA CCGGTGCCTC CCCCGCCTCC TCCTCCAGCC 
CCTGCCTTGC AGGCCCCAGC AGTGCCCCCG CCTCCAGCCC CTCTTCAGAT TGCCCCTGGT 
GTCCTTCACC CAGCTCCTCC TCCAATCGCT CCTCCTCTAG TACAGCCCTC TCCCCCGGTA 
GCCAGAGCCG CCCCAGTGTG TGAGACTGTA CCCGTTCATC CACTCCCACA AGGTGAAGTG 
CAGGGGCTGC CTCCGCCCCC GCCGCCGCCG CCTCTGCCTC CTCCAGGCAT TCGGCCATCA 
TCACCTGTCA CGGTCGCAGC TCTTGCGCAT CCTCCCTCTG GGCTCCACCC AACTCCATCT 
CCTGCCCCTG GTCCCCATGC TCCATTAATG CCTCCGTCCC CACCTTCACA AGTCCTGCCT 
GCCTCTGAGC CAAAGCGCCA TCCTTCCACC CTACCCGTGA TCAGTGACGC GAGGAGTGTG 
CTGCTGGAGG CCATACGGAA AGGCATTCAG CTTCGCAAAG TGGAAGAGCA GCGTGAACAG 
GAAGCAAAGC ATGAGCGGAT TGAAAACGAC GTGGCCACCA TCCTGTCACG CCGCATCGCT 
GTGGAGTACA GCGACTCGGA AGACGACTCG GAGTTCGATG AAGTAGACTG GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wasf1 ( XM_006256586.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256648.1 Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07230
Clone ID Related Accession (Same CDS sequence)	XM_006256586.3 , NM_001025114.1
Accession Version	XM_006256586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
CAGAAAAATC TAGACCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC ACATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCCGATCTC 
CTCCACAAGC ACATCGAAGT TGCTAACGGC CCAGCCTCTC ATTTTGAGAC AAGGCCGCAG 
ACATACGTGG ACCACATGGA TGGATCTTAC TCACTCTCTG CCTTGCCATT CAGTCAGATG 
AGTGAACTCC TAAGCAGAGC TGAGGAGAGG GTCTTAGTCA GACCCCATGA ACCACCTCCA 
CCTCCACCAA TGCATGCAGC AGGGGATGCA AGACCCACCC CCACCTGTGT CAGCTCTGCT 
GCGGGTTTGA TAGAGAATCG TCCCCAGTCC CCAGCTGCAG GCAGAACACC CGTGTTTGTG 
AGCCCCACGC CCCCACCTCC TCCACCACCT CTCCCGTCTG CCCTGTCCAC CTCCTCACTG 
AGAGCTTCCA TGACCTCAAC TCCTCCCCCA CCGGTGCCTC CCCCGCCTCC TCCTCCAGCC 
CCTGCCTTGC AGGCCCCAGC AGTGCCCCCG CCTCCAGCCC CTCTTCAGAT TGCCCCTGGT 
GTCCTTCACC CAGCTCCTCC TCCAATCGCT CCTCCTCTAG TACAGCCCTC TCCCCCGGTA 
GCCAGAGCCG CCCCAGTGTG TGAGACTGTA CCCGTTCATC CACTCCCACA AGGTGAAGTG 
CAGGGGCTGC CTCCGCCCCC GCCGCCGCCG CCTCTGCCTC CTCCAGGCAT TCGGCCATCA 
TCACCTGTCA CGGTCGCAGC TCTTGCGCAT CCTCCCTCTG GGCTCCACCC AACTCCATCT 
CCTGCCCCTG GTCCCCATGC TCCATTAATG CCTCCGTCCC CACCTTCACA AGTCCTGCCT 
GCCTCTGAGC CAAAGCGCCA TCCTTCCACC CTACCCGTGA TCAGTGACGC GAGGAGTGTG 
CTGCTGGAGG CCATACGGAA AGGCATTCAG CTTCGCAAAG TGGAAGAGCA GCGTGAACAG 
GAAGCAAAGC ATGAGCGGAT TGAAAACGAC GTGGCCACCA TCCTGTCACG CCGCATCGCT 
GTGGAGTACA GCGACTCGGA AGACGACTCG GAGTTCGATG AAGTAGACTG GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256648.1
CDS	183..1862
Translation	MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPPPPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

Target ORF information:

RefSeq Version	XM_006256586.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256586.3

ORF Insert Sequence:

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
CAGAAAAATC TAGACCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC ACATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCCGATCTC 
CTCCACAAGC ACATCGAAGT TGCTAACGGC CCAGCCTCTC ATTTTGAGAC AAGGCCGCAG 
ACATACGTGG ACCACATGGA TGGATCTTAC TCACTCTCTG CCTTGCCATT CAGTCAGATG 
AGTGAACTCC TAAGCAGAGC TGAGGAGAGG GTCTTAGTCA GACCCCATGA ACCACCTCCA 
CCTCCACCAA TGCATGCAGC AGGGGATGCA AGACCCACCC CCACCTGTGT CAGCTCTGCT 
GCGGGTTTGA TAGAGAATCG TCCCCAGTCC CCAGCTGCAG GCAGAACACC CGTGTTTGTG 
AGCCCCACGC CCCCACCTCC TCCACCACCT CTCCCGTCTG CCCTGTCCAC CTCCTCACTG 
AGAGCTTCCA TGACCTCAAC TCCTCCCCCA CCGGTGCCTC CCCCGCCTCC TCCTCCAGCC 
CCTGCCTTGC AGGCCCCAGC AGTGCCCCCG CCTCCAGCCC CTCTTCAGAT TGCCCCTGGT 
GTCCTTCACC CAGCTCCTCC TCCAATCGCT CCTCCTCTAG TACAGCCCTC TCCCCCGGTA 
GCCAGAGCCG CCCCAGTGTG TGAGACTGTA CCCGTTCATC CACTCCCACA AGGTGAAGTG 
CAGGGGCTGC CTCCGCCCCC GCCGCCGCCG CCTCTGCCTC CTCCAGGCAT TCGGCCATCA 
TCACCTGTCA CGGTCGCAGC TCTTGCGCAT CCTCCCTCTG GGCTCCACCC AACTCCATCT 
CCTGCCCCTG GTCCCCATGC TCCATTAATG CCTCCGTCCC CACCTTCACA AGTCCTGCCT 
GCCTCTGAGC CAAAGCGCCA TCCTTCCACC CTACCCGTGA TCAGTGACGC GAGGAGTGTG 
CTGCTGGAGG CCATACGGAA AGGCATTCAG CTTCGCAAAG TGGAAGAGCA GCGTGAACAG 
GAAGCAAAGC ATGAGCGGAT TGAAAACGAC GTGGCCACCA TCCTGTCACG CCGCATCGCT 
GTGGAGTACA GCGACTCGGA AGACGACTCG GAGTTCGATG AAGTAGACTG GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wasf1 ( XM_006256587.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256649.1 Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa39547
Clone ID Related Accession (Same CDS sequence)	XM_006256587.3
Accession Version	XM_006256587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
AAAAATCTAG ACCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCACA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC CGATCTCCTC 
CACAAGCACA TCGAAGTTGC TAACGGCCCA GCCTCTCATT TTGAGACAAG GCCGCAGACA 
TACGTGGACC ACATGGATGG ATCTTACTCA CTCTCTGCCT TGCCATTCAG TCAGATGAGT 
GAACTCCTAA GCAGAGCTGA GGAGAGGGTC TTAGTCAGAC CCCATGAACC ACCTCCACCT 
CCACCAATGC ATGCAGCAGG GGATGCAAGA CCCACCCCCA CCTGTGTCAG CTCTGCTGCG 
GGTTTGATAG AGAATCGTCC CCAGTCCCCA GCTGCAGGCA GAACACCCGT GTTTGTGAGC 
CCCACGCCCC CACCTCCTCC ACCACCTCTC CCGTCTGCCC TGTCCACCTC CTCACTGAGA 
GCTTCCATGA CCTCAACTCC TCCCCCACCG GTGCCTCCCC CGCCTCCTCC TCCAGCCCCT 
GCCTTGCAGG CCCCAGCAGT GCCCCCGCCT CCAGCCCCTC TTCAGATTGC CCCTGGTGTC 
CTTCACCCAG CTCCTCCTCC AATCGCTCCT CCTCTAGTAC AGCCCTCTCC CCCGGTAGCC 
AGAGCCGCCC CAGTGTGTGA GACTGTACCC GTTCATCCAC TCCCACAAGG TGAAGTGCAG 
GGGCTGCCTC CGCCCCCGCC GCCGCCGCCT CTGCCTCCTC CAGGCATTCG GCCATCATCA 
CCTGTCACGG TCGCAGCTCT TGCGCATCCT CCCTCTGGGC TCCACCCAAC TCCATCTCCT 
GCCCCTGGTC CCCATGCTCC ATTAATGCCT CCGTCCCCAC CTTCACAAGT CCTGCCTGCC 
TCTGAGCCAA AGCGCCATCC TTCCACCCTA CCCGTGATCA GTGACGCGAG GAGTGTGCTG 
CTGGAGGCCA TACGGAAAGG CATTCAGCTT CGCAAAGTGG AAGAGCAGCG TGAACAGGAA 
GCAAAGCATG AGCGGATTGA AAACGACGTG GCCACCATCC TGTCACGCCG CATCGCTGTG 
GAGTACAGCG ACTCGGAAGA CGACTCGGAG TTCGATGAAG TAGACTGGCT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256649.1
CDS	183..1859
Translation	MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPPPPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

Target ORF information:

RefSeq Version	XM_006256587.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WAS protein family, member 1 (Wasf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256587.3

ORF Insert Sequence:

ATGCCGCTGG TGAAAAGAAA CATCGACCCT AGGCACTTGT GCCACACAGC ACTGCCCAGG 
GGCATTAAGA ACGAGCTGGA ATGTGTGACC AACATCTCCC TGGCAAATAT AATTAGACAA 
CTGAGTAGCC TAAGTAAATA TGCTGAAGAC ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTTAA CTCATTACAA GAGCGCGTGG ACCGATTGTC CGTTAGTGTC 
ACACAGCTTG ATCCTAAAGA AGAAGAACTG TCCTTACAAG ACATAACGAT GAGAAAAGCT 
TTCCGGAGCT CTACAATTCA GGACCAGCAG CTTTTCGACC GCAAGACTTT GCCTATCCCG 
CTGCAGGAAA CGTACGATGT GTGCGAGCAG CCTCCGCCTC TCAATGTCCT CACACCCTAC 
AGGGACGATG GTAAGGAGGG GCTGAAGTTT TATACCAACC CTTCGTATTT CTTTGATCTG 
TGGAAAGAAA AGATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG AAAGCAGAAG 
AAAAATCTAG ACCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCACA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC CGATCTCCTC 
CACAAGCACA TCGAAGTTGC TAACGGCCCA GCCTCTCATT TTGAGACAAG GCCGCAGACA 
TACGTGGACC ACATGGATGG ATCTTACTCA CTCTCTGCCT TGCCATTCAG TCAGATGAGT 
GAACTCCTAA GCAGAGCTGA GGAGAGGGTC TTAGTCAGAC CCCATGAACC ACCTCCACCT 
CCACCAATGC ATGCAGCAGG GGATGCAAGA CCCACCCCCA CCTGTGTCAG CTCTGCTGCG 
GGTTTGATAG AGAATCGTCC CCAGTCCCCA GCTGCAGGCA GAACACCCGT GTTTGTGAGC 
CCCACGCCCC CACCTCCTCC ACCACCTCTC CCGTCTGCCC TGTCCACCTC CTCACTGAGA 
GCTTCCATGA CCTCAACTCC TCCCCCACCG GTGCCTCCCC CGCCTCCTCC TCCAGCCCCT 
GCCTTGCAGG CCCCAGCAGT GCCCCCGCCT CCAGCCCCTC TTCAGATTGC CCCTGGTGTC 
CTTCACCCAG CTCCTCCTCC AATCGCTCCT CCTCTAGTAC AGCCCTCTCC CCCGGTAGCC 
AGAGCCGCCC CAGTGTGTGA GACTGTACCC GTTCATCCAC TCCCACAAGG TGAAGTGCAG 
GGGCTGCCTC CGCCCCCGCC GCCGCCGCCT CTGCCTCCTC CAGGCATTCG GCCATCATCA 
CCTGTCACGG TCGCAGCTCT TGCGCATCCT CCCTCTGGGC TCCACCCAAC TCCATCTCCT 
GCCCCTGGTC CCCATGCTCC ATTAATGCCT CCGTCCCCAC CTTCACAAGT CCTGCCTGCC 
TCTGAGCCAA AGCGCCATCC TTCCACCCTA CCCGTGATCA GTGACGCGAG GAGTGTGCTG 
CTGGAGGCCA TACGGAAAGG CATTCAGCTT CGCAAAGTGG AAGAGCAGCG TGAACAGGAA 
GCAAAGCATG AGCGGATTGA AAACGACGTG GCCACCATCC TGTCACGCCG CATCGCTGTG 
GAGTACAGCG ACTCGGAAGA CGACTCGGAG TTCGATGAAG TAGACTGGCT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth.
Molecular biology of the cell27(21)3342-3356(2016 Nov)
Xu C,Fu X,Zhu S,Liu JJ

A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs.
Biochemistry54(1)11-21(2015 Jan)
Burgers PP,van der Heyden MA,Kok B,Heck AJ,Scholten A

Structure and control of the actin regulatory WAVE complex.
Nature468(7323)533-8(2010 Nov)
Chen Z,Borek D,Padrick SB,Gomez TS,Metlagel Z,Ismail AM,Umetani J,Billadeau DD,Otwinowski Z,Rosen MK

Rapid signaling of estrogen to WAVE1 and moesin controls neuronal spine formation via the actin cytoskeleton.
Molecular endocrinology (Baltimore, Md.)23(8)1193-202(2009 Aug)
Sanchez AM,Flamini MI,Fu XD,Mannella P,Giretti MS,Goglia L,Genazzani AR,Simoncini T

WAVE1 controls neuronal activity-induced mitochondrial distribution in dendritic spines.
Proceedings of the National Academy of Sciences of the United States of America105(8)3112-6(2008 Feb)
Sung JY,Engmann O,Teylan MA,Nairn AC,Greengard P,Kim Y

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Wasf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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