Map1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map1b
Entrez Gene ID	29456
Full Name	microtubule-associated protein 1B
Synonyms	Mtap1b
General protein information	Preferred Names microtubule-associated protein 1B Names microtubule-associated protein 1B MAP-1B neuraxin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	binds microtubules and may function as microtubule cross-linkers [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019217.1	NP_062090.1	microtubule-associated protein 1B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP1B	NP_001181849.1
Homo sapiens (human)	MAP1B	NP_005900.2
Mus musculus (house mouse)	Map1b	NP_032660.2
Canis lupus familiaris (dog)	MAP1B	XP_535269.3
Rattus norvegicus (Norway rat)	Map1b	NP_062090.1
	MAP1B	XP_001097124.2

Gene Ontology
Bibliography

Related articles in PubMed

MAP1B Light Chain Modulates Synaptic Transmission via AMPA Receptor Intracellular Trapping.
Palenzuela R, Guti?rrez Y, Draffin JE, Lario A, Benoist M, Esteban JA
The Journal of neuroscience : the official journal of the Society for Neuroscience37(41)9945-9963(2017 Oct)

Dynamic Palmitoylation Targets MAP6 to the Axon to Promote Microtubule Stabilization during Neuronal Polarization.
Tortosa E, Adolfs Y, Fukata M, Pasterkamp RJ, Kapitein LC, Hoogenraad CC
Neuron94(4)809-825.e7(2017 May)

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC
Neuron94(2)347-362.e7(2017 Apr)

Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration.
Liu Y, Lee JW, Ackerman SL
The Journal of neuroscience : the official journal of the Society for Neuroscience35(11)4587-98(2015 Mar)

Interaction of tubby-like protein-1 (Tulp1) and microtubule-associated protein (MAP) 1A and MAP1B in the mouse retina.
Grossman GH, Beight CD, Ebke LA, Pauer GJ, Hagstrom SA
Advances in experimental medicine and biology801511-8(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MAP1B restricts the access of AMPARs to dendritic spines and the postsynaptic membrane, contributing to downregulating synaptic transmission
Title: MAP1B Light Chain Modulates Synaptic Transmission via AMPA Receptor Intracellular Trapping.

MAP1B enhances microtubule assembly rates and axon extension rates in developing neurons by binding to dynamic microtubules.
Title: MAP1B enhances microtubule assembly rates and axon extension rates in developing neurons.

MAP1B interacts with a guanine exchange factor, termed Tiam1, which specifically activates Rac1. a novel function of MAP1B related with the control of small GTPases
Title: The light chain 1 subunit of the microtubule-associated protein 1B (MAP1B) is responsible for Tiam1 binding and Rac1 activation in neuronal cells.

Overexpression of MAP1B 1-126 induced both neurite extension and neuronal death, suggesting that MAP1B 1-126 could be involved in neuronal degeneration and aberrant sprouting.
Title: MAP1B 1-126 interacts with tubulin isoforms and induces neurite outgrowth and neuronal death of cultured cortical neurons.

We suggest a role for Stau2 in the generation and regulation of Map1b mRNA containing granules that are required for mGluR-long-term depression
Title: Staufen 2 regulates mGluR long-term depression and Map1b mRNA distribution in hippocampal neurons.

The following Map1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04060	NM_019217.1 Latest version!	Rattus norvegicus microtubule-associated protein 1B (Map1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$2169.30 $3099.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map1b ( NM_019217.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062090.1 Rattus norvegicus microtubule-associated protein 1B (Map1b), mRNA.

Price & Availability↑

CloneID	ORa04060
Clone ID Related Accession (Same CDS sequence)	NM_019217.1
Accession Version	NM_019217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated protein 1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CB610210.1, X60370.1, BF286433.1, CF977184.1, BG665276.1, CB579120.1, CB583564.1, BG378086.1, CB810012.1, CB584802.1, CA510735.1, CB580055.1, CA510940.1, CO393350.1, CB576609.1, AA925503.1 and CA503854.1. On or before Oct 28, 2007 this sequence version replaced XM_215469.4, XM_001061557.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCG TGGTGGTGGA AGCCACCGAG CCGGAGCCAT CGGGCAGCAT CGGCAACCCG 
GCGGCGACCA CCTCGCCCAG CCTGTCGCAC CGCTTCCTAG ACAGCAAGTT CTACTTGCTG 
GTGGTGGTCG GCGAGACGGT GACCGAAGAG CACCTGAGGC GTGCCATCGG CAACATCGAG 
CTGGGGATCC GATCGTGGGA CACAAACCTG ATCGAGTGCA ACTTGGACCA AGAGCTCAAA 
CTTTTCGTGT CTCGACACTC CGCGAGATTC TCTCCTGAAG TTCCAGGACA AAAGATCCTC 
CATCACCGAA GTGACGTCTT AGAAACTGTA GTTCTGATCA ACCCTTCGGA TGAAGCAGTC 
AGCACCGAGG TGCGTTTGAT GATCACTGAC GCCGCCCGCC ATAAACTGCT GGTGCTCACC 
GGACAGTGCT TTGAGAACAC TGGAGAGCTC ATCCTCCAGT CAGGCTCTTT CTCCTTCCAG 
AACTTCATAG AGATTTTCAC CGACCAAGAG ATTGGGGAGC TCCTAAGCAC CACCCATCCT 
GCCAACAAAG CCAGCCTCAC CCTCTTCTGC CCTGAGGAAG GAGACTGGAA GAACTCCAAC 
CTTGACAGAC ACAATCTCCA AGACTTCATC AACATCAAGC TCAACTCAGC TTCTATCTTG 
CCAGAAATGG AGGGACTTTC TGAGTTCACC GAGTACCTCT CGGAGTCTGT CGAAGTCCCC 
TCCCCCTTTG ACATCCTGGA GCCCCCGACC TCGGGCGGAT TTCTGAAGCT CTCCAAGCCT 
TGTTGTTACA TTTTTCCGGG GGGCCGCGGG GACTCTGCCC TGTTCGCAGT GAACGGATTC 
AACATGCTCA TTAACGGAGG ATCAGAAAGA AAGTCCTGCT TCTGGAAGCT CATTCGGCAC 
TTGGACCGGG TGGACTCCAT CCTGCTCACC CACATTGGGG ATGACAACTT GCCCGGGATC 
AACAGCATGT TGCAACGCAA GATTGCAGAG CTGGAAGAGG AGCGGTCCCA GGGCTCCACC 
AGCAACAGTG ACTGGATGAA AAACCTCATC TCCCCTGACT TGGGGGTTGT GTTTCTCAAT 
GTACCTGAAA ATCTGAAAAA CCCAGAACCC AACATCAAGA TGAAGAGAAG TACAGAAGAA 
GCATGCTTCA CCCTCCAGTA CCTAAACAAA CTGTCCATGA AACCAGAGCC TTTATTTAGA 
AGTGTAGGCA ATGCCATTGA GCCTGTCATC CTGTTCCAAA AAATGGGAGT GGGTAAACTG 
GAGATGTACG TGCTTAACCC AGTCAAAAGC AGCAAGGAAA TGCAGTATTT CATGCAGCAG 
TGGACTGGAA CCAACAAAGA CAAGGCTGAA CTTATCCTGC CCAATGGTCA AGAAGTAGAC 
ATCCCGATTT CCTACCTGAC TTCCGTCTCG TCTTTGATTG TGTGGCACCC AGCCAACCCT 
GCTGAGAAAA TCATCCGGGT TCTGTTTCCT GGAAACAGCA CCCAGTACAA CATCCTAGAA 
GGGCTGGAAA AACTCAAACA TCTAGACTTC CTAAAGCAGC CACTGGCCAC CCAAAAAGAT 
CTCACTGGCC AGGTGTCCAC CCCCCCAGTG AAACAGGTCA AGTTGAAACA GCGGGCTGAC 
AGCCGAGAGA GTCTGAAGCC AGCCACAAAA CCACTTTCCA GTAAATCAGT GAGGAAGGAG 
TCCAAAGAGG AGGCCCCTGA AGCCACAAAA GCCAGCCAAG TGGAAAAAAC ACCCAAAGTT 
GAAAGCAAAG AGAAAGTGAT AGTGAAAAAA GACAAGCCAG GAAAGGTAGA AAGTAAGCCA 
TCGGTGACGG AAAAGGAGGT GCCCAGCAAA GAGGAGCAGT CGCCCGTCAA AGCTGAGGTG 
GCTGAGAAGG CGGCCACGGA GAGCAAACCC AAAGTCACCA AAGACAAAGT GGTAAAAAAG 
GAAATAAAGA CAAAACCCGA AGAAAAGAAA GAGGAGAAGC CCAAGAAGGA AGTGGCTAAA 
AAGGAAGACA AAACTCCCCT CAAGAAAGAC GAGAAGCCCA AAAAGGAAGA GGCGAAGAAG 
GAGATCAAGA AAGAAATCAA AAAGGAAGAG AAAAAGGAGC TGAAGAAAGA GGTGAAGAAG 
GAAACGCCCC TGAAGGACGC CAAGAAGGAG GTGAAGAAAG ACGAGAAGAA AGAAGTTAAA 
AAGGAAGAGA AGGAACCCAA AAAGGAGATT AAGAAGATCT CCAAGGACAT AAAGAAATCC 
ACTCCTCTGT CAGACACAAA GAAACCGGCT GCATTGAAAC CAAAAGTAGC AAAGAAAGAA 
GAGCCCACCA AGAAGGAGCC TATTGCTGCT GGGAAACTCA AGGACAAGGG GAAGGTCAAA 
GTCATTAAAA AGGAAGGCAA GACCACAGAG GCCGCTGCCA CAGCTGTTGG CACTGCTGCC 
GTGGCTGCAG CAGCCGGAGT AGCGGCCAGC GGTCCTGCCA AAGAACTTGA AGCTGAGCGG 
TCCCTCATGT CGTCCCCTGA GGATCTAACC AAGGACTTTG AGGAGCTAAA GGCTGAGGAG 
ATCGATGTAG CGAAGGACAT CAAGCCTCAG CTGGAGCTCA TTGAAGATGA AGAGAAACTG 
AAGGAAACCG AGCCGGGAGA AGCCTACGTC ATTCAGAAAG AGACGGAAGT CAGCAAAGGT 
TCTGCTGAGT CACCTGATGA AGGGATCACC ACCACTGAGG GGGAAGGGGA GTGCGAGCAA 
ACCCCCGAGG AGCTGGAGCC AGTTGAGAAG CAGGGCGTGG ATGACATCGA GAAGTTCGAG 
GATGAAGGCG CTGGTTTTGA AGAATCCTCA GAGGCCGGAG ACTACGAAGA GAAGGCAGAA 
ACTGAGGAGG CCGAGGAGCC GGAAGAAGAC GGGGAAGACA ATGTGAGCGG GAGCGCCTCG 
AAGCACAGCC CCACAGAAGA CGAAGAAATC GCTAAGGCTG AGGCGGACGT ACACATCAAG 
GAGAAGAGGG AGTCTGTGGC CAGCGGCGAT GACCGGGCCG AAGAAGACAT GGATGAAGCG 
CTTGAGAAAG GAGAAGCTGA ACAGTCTGAG GAGGAGGGTG AGGAGGAGGA GGACAAAGCA 
GAGGACGCCA GAGAGGAAGA CCATGAGCCC GACAAAACTG AGGCTGAAGA TTATGTGATG 
GCTGTGGTTG ACAAGGCCGC GGAGGCCGGA GTCACCGAGG ATCAGTATGA TTTCCTGGGG 
ACACCGGCCA AGCAACCTGG AGTCCAGTCT CCTAGCCGAG AACCCGCGTC TTCAATTCAT 
GATGAGACCC TACCCGGAGG CTCCGAGAGC GAGGCCACTG CTTCAGATGA GGAGAATCGA 
GAAGACCAGC CTGAGGAATT CACTGCTACC TCCGGATATA CTCAGTCCAC CATCGAGATA 
TCTAGTGAGC CGACTCCAAT GGATGAGATG TCCACTCCTC GAGATGTGAT GACCGACGAG 
ACCAACAATG AGGAGACAGA GTCCCCGTCT CAGGAGTTCG TGAACATTAC CAAATACGAG 
TCTTCGCTGT ACTCTCAGGA GTACTCCAAA CCTGTGGTTG CATCATTCAA TGGATTGTCA 
GACGGGTCAA AGACAGACGC CACTGACGGT AGGGATTACA ACGCTTCCGC CTCCACCATA 
TCACCACCTT CGTCCATGGA AGAAGACAAA TTCAGCAAGT CTGCTCTTCG TGACGCTTAC 
CGCCCAGAAG AGACGGACGT GAAAACCGGT GCCGAGTTGG ACATCAAAGA TGTTTCGGAT 
GAGAGACTTA GCCCAGCCAA GAGTCCATCC CTGAGTCCTT CTCCACCATC ACCCATAGAG 
AAGACTCCCC TGGGTGAACG TAGCGTGAAT TTCTCTCTGA CACCCAACGA GATCAAAGCC 
TCTGCAGAGG GAGAGGCAAC AGCAGTAGTG TCCCCCGGAG TGACCCAAGC AGTAGTTGAA 
GAACACTGTG CCAGTCCTGA GGAGAAGACC TTGGAGGTAG TGTCACCGTC TCAGTCTGTG 
ACAGGCAGTG CGGGCCACAC ACCTTACTAC CAATCTCCCA CCGACGAAAA GTCCAGTCAC 
CTACCTACAG AAGTCACTGA GAACGCGCAG GCAGTCCCGG TGAGCTTTGA ATTCACTGAG 
GCCAAAGATG AGAACGAGAG GTCGTCCATC AGCCCCATGG ATGAACCTGT GCCTGACTCA 
GAGTCTCCTA TCGAGAAAGT TCTGTCTCCG TTACGCAGCC CTCCCCTTAT TGGATCCGAG 
TCCGCATATG AAGACTTCCT GAGTGCGGAT GACAAGGCTC TTGGCAGACG TTCAGAAAGC 
CCCTTTGAAG GGAAGAATGG AAAGCAAGGC TTCTCAGACA AAGAAAGCCC AGTTTCTGAC 
CTGACTTCCG ATCTTTACCA AGACAAGCAG GAAGAGAAAA GCGCGGGCTT CATACCGATA 
AAGGAAGACT TTAGTCCAGA AAAGAAAGCC AGCGATGCTG AAATCATGAG TTCTCAATCA 
GCTCTGGCTT TGGATGAAAG GAAACTGGGA GGAGATGGAT CTCCAACGCA AGTAGATGTC 
AGTCAGTTTG GCTCTTTCAA AGAAGACACC AAGATGTCCA TTTCGGAAGG CACCGTTTCA 
GACAAGTCCG CCACGCCTGT GGATGAGGGC GTGGCCGAAG ACACCTATTC ACACATGGAA 
GGTGTGGCCT CAGTGTCAAC CGCCTCTGTG GCTACCAGCT CGTTTCCAGA GCCAACCACA 
GATGACGTGT CTCCTTCTCT CCACGCTGAA GTGGGCTCTC CACATTCCAC AGAGGTGGAT 
GACTCCCTGT CGGTGTCGGT GGTGCAAACA CCAACTACTT TCCAGGAAAC AGAAATGTCT 
CCGTCTAAAG AAGAGTGCCC AAGACCAATG TCGATTTCTC CTCCTGACTT CTCCCCTAAG 
ACAGCCAAAT CCAGGACACC AGTTCAAGAT CACCGATCCG AACAGTCTTC AATGTCTATT 
GAATTCGGTC AGGAATCCCC CGAGCATTCT CTTGCTATGG ACTTTAGTCG GCAGTCTCCA 
GACCACCCTA CTGTGGGTGC TGGTATGCTT CACATCACCG AAAATGGGCC AACTGAGGTG 
GACTACAGTC CCTCCGATAT CCAGGACTCT AGTTTGTCAC ATAAGATTCC GCCGACAGAA 
GAGCCATCCT ACACCCAGGA TAATGATCTG TCCGAGCTCA TCTCTGTGTC TCAGGTGGAG 
GCTTCCCCAT CCACCTCTTC TGCTCACACT CCTTCTCAGA TAGCCTCTCC TCTTCAGGAA 
GACACTCTCT CTGATGTCGT TCCTCCCAGA GATATGTCCT TATATGCCTC GCTTGCGTCT 
GAGAAAGTGC AGAGCCTGGA AGGAGAGAAA CTCTCTCCAA AATCCGATAT TTCTCCGCTC 
ACCCCTCGAG AGTCCTCACC TACATATTCA CCTGGCTTTT CAGATTCTAC CTCTGGAGCT 
AAAGAGAGTA CAGCGGCTTA CCAAACCTCC TCTTCCCCAC CAATAGATGC AGCAGCCGCA 
GAGCCCTACG GCTTCCGCTC CTCAATGTTA TTTGATACAA TGCAGCATCA CCTGGCCTTG 
AGTAGAGATT TGACCACATC TAGTGTGGAG AAGGACAATG GAGGGAAGAC ACCCGGTGAC 
TTTAACTATG CCTATCAAAA GCCCGAGAGC ACCACCGAAT CCCCAGATGA AGAAGATTAT 
GACTATGAAT CTCACGAGAA AACCATCCAG GCCCACGATG TGGGTGGTTA CTACTATGAG 
AAGACAGAGA GAACCATAAA ATCCCCATGT GACAGTGGAT ACTCCTATGA GACCATTGAG 
AAGACCACCA AGACCCCAGA AGATGGTGGC TACTCCTGTG AAATTACCGA GAAAACCACT 
CGGACCCCTG AAGAGGGCGG GTACTCGTAT GAGATCAGCG AGAAGACAAC ACGAACCCCT 
GAAGTAAGTG GCTACACCTA TGAGAAGACC GAGAGGTCCA GAAGGCTCCT CGATGACATT 
AGCAATGGCT ACGATGACAC TGAGGATGGT GGCCACACAC TTGGCGACTG TAGCTATTCC 
TACGAAACCA CTGAGAAAAT TACCAGCTTT CCTGAATCTG AAAGCTATTC CTATGAGACA 
ACTACAAAAA CAACACGGAG TCCAGACACC TCTGCATACT GTTACGAGAC CATGGAGAAG 
ATCACCAAGA CCCCACAGGC ATCCACATAC TCCTATGAGA CCTCAGACCG ATGCTACACT 
CCAGAAAGGA AGTCCCCCTC GGAGGCACGC CAGGATGTTG ACTTGTGTCT GGTGTCCTCC 
TGTGAATTCA AGCATCCCAA GACCGAGCTC TCACCTTCCT TCATTAATCC AAACCCTCTC 
GAGTGGTTTG CTGGGGAAGA GCCCACTGAA GAATCTGAGA AGCCTCTCAC TCAGTCTGGA 
GGAGCCCCCC CACCTTCAGG AGGAAAACAA CAGGGCAGAC AATGCGATGA AACTCCACCC 
ACCTCAGTCA GTGAGTCAGC TCCATCCCAG ACGGACTCTG ATGTTCCCCC AGAGACAGAA 
GAGTGCCCCT CCATCACAGC TGATGCCAAC ATTGACTCTG AAGATGAGTC AGAAACCATC 
CCCACAGACA AAACGGTTAC GTACAAACAC ATGGACCCGC CTCCAGCCCC CATGCAAGAC 
CGAAGCCCTT CTCCTCGCCA CCCTGATGTG TCCATGGTGG ATCCAGAGGC CTTGGCTATT 
GAGCAGAACC TAGGCAAGGC TCTGAAAAAG GATCTGAAGG AGAAGGCCAA GACCAAGAAA 
CCAGGCACAA AGACCAAGTC CTCTTCACCT GTCAAAAAGG GTGATGGGAA GTCCAAGCCT 
TCAGCAGCTT CCCCCAAACC AGGAGCCTTG AAGGAATCCT CTGACAAGGT GTCCAGAGTG 
GCTTCTCCCA AGAAGAAAGA GTCTGTGGAG AAAGCTATGA AGACCACCAC CACTCCTGAG 
GTCAAAGCCA CACGAGGGGA AGAGAAGGAC AAGGAAACTA AGAATGCAGC CAATGCTTCT 
GCATCCAAGT CAGTGAAGAC TGCAACAGCA GGACCAGGAA CCACTAAGAC GGCCAAGTCG 
TCCACCGTGC CTCCCGGCCT CCCTGTGTAT TTGGACCTCT GCTATATTCC CAACCACAGC 
AACAGTAAGA ATGTCGATGT TGAGTTTTTC AAGAGAGTGA GGTCATCTTA CTACGTGGTG 
AGTGGGAACG ACCCTGCCGC GGAGGAGCCC AGCCGGGCTG TCCTGGATGC CTTGTTGGAA 
GGGAAGGCTC AGTGGGGAAG CAACATGCAG GTGACTCTGA TCCCAACACA TGACTCTGAG 
GTGATGAGGG AGTGGTACCA GGAGACCCAC GAGAAGCAGC AAGACCTCAA CATCATGGTC 
CTAGCAAGCA GTAGTACAGT GGTCATGCAA GACGAGTCCT TCCCTGCATG CAAGATAGAA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062090.1
CDS	165..7550
Translation	MATVVVEATEPEPSGSIGNPAATTSPSLSHRFLDSKFYLLVVVGETVTEEHLRRAIGNIELGIRSWDTNLIECNLDQELKLFVSRHSARFSPEVPGQKILHHRSDVLETVVLINPSDEAVSTEVRLMITDAARHKLLVLTGQCFENTGELILQSGSFSFQNFIEIFTDQEIGELLSTTHPANKASLTLFCPEEGDWKNSNLDRHNLQDFINIKLNSASILPEMEGLSEFTEYLSESVEVPSPFDILEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGFNMLINGGSERKSCFWKLIRHLDRVDSILLTHIGDDNLPGINSMLQRKIAELEEERSQGSTSNSDWMKNLISPDLGVVFLNVPENLKNPEPNIKMKRSTEEACFTLQYLNKLSMKPEPLFRSVGNAIEPVILFQKMGVGKLEMYVLNPVKSSKEMQYFMQQWTGTNKDKAELILPNGQEVDIPISYLTSVSSLIVWHPANPAEKIIRVLFPGNSTQYNILEGLEKLKHLDFLKQPLATQKDLTGQVSTPPVKQVKLKQRADSRESLKPATKPLSSKSVRKESKEEAPEATKASQVEKTPKVESKEKVIVKKDKPGKVESKPSVTEKEVPSKEEQSPVKAEVAEKAATESKPKVTKDKVVKKEIKTKPEEKKEEKPKKEVAKKEDKTPLKKDEKPKKEEAKKEIKKEIKKEEKKELKKEVKKETPLKDAKKEVKKDEKKEVKKEEKEPKKEIKKISKDIKKSTPLSDTKKPAALKPKVAKKEEPTKKEPIAAGKLKDKGKVKVIKKEGKTTEAAATAVGTAAVAAAAGVAASGPAKELEAERSLMSSPEDLTKDFEELKAEEIDVAKDIKPQLELIEDEEKLKETEPGEAYVIQKETEVSKGSAESPDEGITTTEGEGECEQTPEELEPVEKQGVDDIEKFEDEGAGFEESSEAGDYEEKAETEEAEEPEEDGEDNVSGSASKHSPTEDEEIAKAEADVHIKEKRESVASGDDRAEEDMDEALEKGEAEQSEEEGEEEEDKAEDAREEDHEPDKTEAEDYVMAVVDKAAEAGVTEDQYDFLGTPAKQPGVQSPSREPASSIHDETLPGGSESEATASDEENREDQPEEFTATSGYTQSTIEISSEPTPMDEMSTPRDVMTDETNNEETESPSQEFVNITKYESSLYSQEYSKPVVASFNGLSDGSKTDATDGRDYNASASTISPPSSMEEDKFSKSALRDAYRPEETDVKTGAELDIKDVSDERLSPAKSPSLSPSPPSPIEKTPLGERSVNFSLTPNEIKASAEGEATAVVSPGVTQAVVEEHCASPEEKTLEVVSPSQSVTGSAGHTPYYQSPTDEKSSHLPTEVTENAQAVPVSFEFTEAKDENERSSISPMDEPVPDSESPIEKVLSPLRSPPLIGSESAYEDFLSADDKALGRRSESPFEGKNGKQGFSDKESPVSDLTSDLYQDKQEEKSAGFIPIKEDFSPEKKASDAEIMSSQSALALDERKLGGDGSPTQVDVSQFGSFKEDTKMSISEGTVSDKSATPVDEGVAEDTYSHMEGVASVSTASVATSSFPEPTTDDVSPSLHAEVGSPHSTEVDDSLSVSVVQTPTTFQETEMSPSKEECPRPMSISPPDFSPKTAKSRTPVQDHRSEQSSMSIEFGQESPEHSLAMDFSRQSPDHPTVGAGMLHITENGPTEVDYSPSDIQDSSLSHKIPPTEEPSYTQDNDLSELISVSQVEASPSTSSAHTPSQIASPLQEDTLSDVVPPRDMSLYASLASEKVQSLEGEKLSPKSDISPLTPRESSPTYSPGFSDSTSGAKESTAAYQTSSSPPIDAAAAEPYGFRSSMLFDTMQHHLALSRDLTTSSVEKDNGGKTPGDFNYAYQKPESTTESPDEEDYDYESHEKTIQAHDVGGYYYEKTERTIKSPCDSGYSYETIEKTTKTPEDGGYSCEITEKTTRTPEEGGYSYEISEKTTRTPEVSGYTYEKTERSRRLLDDISNGYDDTEDGGHTLGDCSYSYETTEKITSFPESESYSYETTTKTTRSPDTSAYCYETMEKITKTPQASTYSYETSDRCYTPERKSPSEARQDVDLCLVSSCEFKHPKTELSPSFINPNPLEWFAGEEPTEESEKPLTQSGGAPPPSGGKQQGRQCDETPPTSVSESAPSQTDSDVPPETEECPSITADANIDSEDESETIPTDKTVTYKHMDPPPAPMQDRSPSPRHPDVSMVDPEALAIEQNLGKALKKDLKEKAKTKKPGTKTKSSSPVKKGDGKSKPSAASPKPGALKESSDKVSRVASPKKKESVEKAMKTTTTPEVKATRGEEKDKETKNAANASASKSVKTATAGPGTTKTAKSSTVPPGLPVYLDLCYIPNHSNSKNVDVEFFKRVRSSYYVVSGNDPAAEEPSRAVLDALLEGKAQWGSNMQVTLIPTHDSEVMREWYQETHEKQQDLNIMVLASSSTVVMQDESFPACKIEL

Target ORF information:

RefSeq Version	NM_019217.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein 1B (Map1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019217.1

ORF Insert Sequence:

ATGGCGACCG TGGTGGTGGA AGCCACCGAG CCGGAGCCAT CGGGCAGCAT CGGCAACCCG 
GCGGCGACCA CCTCGCCCAG CCTGTCGCAC CGCTTCCTAG ACAGCAAGTT CTACTTGCTG 
GTGGTGGTCG GCGAGACGGT GACCGAAGAG CACCTGAGGC GTGCCATCGG CAACATCGAG 
CTGGGGATCC GATCGTGGGA CACAAACCTG ATCGAGTGCA ACTTGGACCA AGAGCTCAAA 
CTTTTCGTGT CTCGACACTC CGCGAGATTC TCTCCTGAAG TTCCAGGACA AAAGATCCTC 
CATCACCGAA GTGACGTCTT AGAAACTGTA GTTCTGATCA ACCCTTCGGA TGAAGCAGTC 
AGCACCGAGG TGCGTTTGAT GATCACTGAC GCCGCCCGCC ATAAACTGCT GGTGCTCACC 
GGACAGTGCT TTGAGAACAC TGGAGAGCTC ATCCTCCAGT CAGGCTCTTT CTCCTTCCAG 
AACTTCATAG AGATTTTCAC CGACCAAGAG ATTGGGGAGC TCCTAAGCAC CACCCATCCT 
GCCAACAAAG CCAGCCTCAC CCTCTTCTGC CCTGAGGAAG GAGACTGGAA GAACTCCAAC 
CTTGACAGAC ACAATCTCCA AGACTTCATC AACATCAAGC TCAACTCAGC TTCTATCTTG 
CCAGAAATGG AGGGACTTTC TGAGTTCACC GAGTACCTCT CGGAGTCTGT CGAAGTCCCC 
TCCCCCTTTG ACATCCTGGA GCCCCCGACC TCGGGCGGAT TTCTGAAGCT CTCCAAGCCT 
TGTTGTTACA TTTTTCCGGG GGGCCGCGGG GACTCTGCCC TGTTCGCAGT GAACGGATTC 
AACATGCTCA TTAACGGAGG ATCAGAAAGA AAGTCCTGCT TCTGGAAGCT CATTCGGCAC 
TTGGACCGGG TGGACTCCAT CCTGCTCACC CACATTGGGG ATGACAACTT GCCCGGGATC 
AACAGCATGT TGCAACGCAA GATTGCAGAG CTGGAAGAGG AGCGGTCCCA GGGCTCCACC 
AGCAACAGTG ACTGGATGAA AAACCTCATC TCCCCTGACT TGGGGGTTGT GTTTCTCAAT 
GTACCTGAAA ATCTGAAAAA CCCAGAACCC AACATCAAGA TGAAGAGAAG TACAGAAGAA 
GCATGCTTCA CCCTCCAGTA CCTAAACAAA CTGTCCATGA AACCAGAGCC TTTATTTAGA 
AGTGTAGGCA ATGCCATTGA GCCTGTCATC CTGTTCCAAA AAATGGGAGT GGGTAAACTG 
GAGATGTACG TGCTTAACCC AGTCAAAAGC AGCAAGGAAA TGCAGTATTT CATGCAGCAG 
TGGACTGGAA CCAACAAAGA CAAGGCTGAA CTTATCCTGC CCAATGGTCA AGAAGTAGAC 
ATCCCGATTT CCTACCTGAC TTCCGTCTCG TCTTTGATTG TGTGGCACCC AGCCAACCCT 
GCTGAGAAAA TCATCCGGGT TCTGTTTCCT GGAAACAGCA CCCAGTACAA CATCCTAGAA 
GGGCTGGAAA AACTCAAACA TCTAGACTTC CTAAAGCAGC CACTGGCCAC CCAAAAAGAT 
CTCACTGGCC AGGTGTCCAC CCCCCCAGTG AAACAGGTCA AGTTGAAACA GCGGGCTGAC 
AGCCGAGAGA GTCTGAAGCC AGCCACAAAA CCACTTTCCA GTAAATCAGT GAGGAAGGAG 
TCCAAAGAGG AGGCCCCTGA AGCCACAAAA GCCAGCCAAG TGGAAAAAAC ACCCAAAGTT 
GAAAGCAAAG AGAAAGTGAT AGTGAAAAAA GACAAGCCAG GAAAGGTAGA AAGTAAGCCA 
TCGGTGACGG AAAAGGAGGT GCCCAGCAAA GAGGAGCAGT CGCCCGTCAA AGCTGAGGTG 
GCTGAGAAGG CGGCCACGGA GAGCAAACCC AAAGTCACCA AAGACAAAGT GGTAAAAAAG 
GAAATAAAGA CAAAACCCGA AGAAAAGAAA GAGGAGAAGC CCAAGAAGGA AGTGGCTAAA 
AAGGAAGACA AAACTCCCCT CAAGAAAGAC GAGAAGCCCA AAAAGGAAGA GGCGAAGAAG 
GAGATCAAGA AAGAAATCAA AAAGGAAGAG AAAAAGGAGC TGAAGAAAGA GGTGAAGAAG 
GAAACGCCCC TGAAGGACGC CAAGAAGGAG GTGAAGAAAG ACGAGAAGAA AGAAGTTAAA 
AAGGAAGAGA AGGAACCCAA AAAGGAGATT AAGAAGATCT CCAAGGACAT AAAGAAATCC 
ACTCCTCTGT CAGACACAAA GAAACCGGCT GCATTGAAAC CAAAAGTAGC AAAGAAAGAA 
GAGCCCACCA AGAAGGAGCC TATTGCTGCT GGGAAACTCA AGGACAAGGG GAAGGTCAAA 
GTCATTAAAA AGGAAGGCAA GACCACAGAG GCCGCTGCCA CAGCTGTTGG CACTGCTGCC 
GTGGCTGCAG CAGCCGGAGT AGCGGCCAGC GGTCCTGCCA AAGAACTTGA AGCTGAGCGG 
TCCCTCATGT CGTCCCCTGA GGATCTAACC AAGGACTTTG AGGAGCTAAA GGCTGAGGAG 
ATCGATGTAG CGAAGGACAT CAAGCCTCAG CTGGAGCTCA TTGAAGATGA AGAGAAACTG 
AAGGAAACCG AGCCGGGAGA AGCCTACGTC ATTCAGAAAG AGACGGAAGT CAGCAAAGGT 
TCTGCTGAGT CACCTGATGA AGGGATCACC ACCACTGAGG GGGAAGGGGA GTGCGAGCAA 
ACCCCCGAGG AGCTGGAGCC AGTTGAGAAG CAGGGCGTGG ATGACATCGA GAAGTTCGAG 
GATGAAGGCG CTGGTTTTGA AGAATCCTCA GAGGCCGGAG ACTACGAAGA GAAGGCAGAA 
ACTGAGGAGG CCGAGGAGCC GGAAGAAGAC GGGGAAGACA ATGTGAGCGG GAGCGCCTCG 
AAGCACAGCC CCACAGAAGA CGAAGAAATC GCTAAGGCTG AGGCGGACGT ACACATCAAG 
GAGAAGAGGG AGTCTGTGGC CAGCGGCGAT GACCGGGCCG AAGAAGACAT GGATGAAGCG 
CTTGAGAAAG GAGAAGCTGA ACAGTCTGAG GAGGAGGGTG AGGAGGAGGA GGACAAAGCA 
GAGGACGCCA GAGAGGAAGA CCATGAGCCC GACAAAACTG AGGCTGAAGA TTATGTGATG 
GCTGTGGTTG ACAAGGCCGC GGAGGCCGGA GTCACCGAGG ATCAGTATGA TTTCCTGGGG 
ACACCGGCCA AGCAACCTGG AGTCCAGTCT CCTAGCCGAG AACCCGCGTC TTCAATTCAT 
GATGAGACCC TACCCGGAGG CTCCGAGAGC GAGGCCACTG CTTCAGATGA GGAGAATCGA 
GAAGACCAGC CTGAGGAATT CACTGCTACC TCCGGATATA CTCAGTCCAC CATCGAGATA 
TCTAGTGAGC CGACTCCAAT GGATGAGATG TCCACTCCTC GAGATGTGAT GACCGACGAG 
ACCAACAATG AGGAGACAGA GTCCCCGTCT CAGGAGTTCG TGAACATTAC CAAATACGAG 
TCTTCGCTGT ACTCTCAGGA GTACTCCAAA CCTGTGGTTG CATCATTCAA TGGATTGTCA 
GACGGGTCAA AGACAGACGC CACTGACGGT AGGGATTACA ACGCTTCCGC CTCCACCATA 
TCACCACCTT CGTCCATGGA AGAAGACAAA TTCAGCAAGT CTGCTCTTCG TGACGCTTAC 
CGCCCAGAAG AGACGGACGT GAAAACCGGT GCCGAGTTGG ACATCAAAGA TGTTTCGGAT 
GAGAGACTTA GCCCAGCCAA GAGTCCATCC CTGAGTCCTT CTCCACCATC ACCCATAGAG 
AAGACTCCCC TGGGTGAACG TAGCGTGAAT TTCTCTCTGA CACCCAACGA GATCAAAGCC 
TCTGCAGAGG GAGAGGCAAC AGCAGTAGTG TCCCCCGGAG TGACCCAAGC AGTAGTTGAA 
GAACACTGTG CCAGTCCTGA GGAGAAGACC TTGGAGGTAG TGTCACCGTC TCAGTCTGTG 
ACAGGCAGTG CGGGCCACAC ACCTTACTAC CAATCTCCCA CCGACGAAAA GTCCAGTCAC 
CTACCTACAG AAGTCACTGA GAACGCGCAG GCAGTCCCGG TGAGCTTTGA ATTCACTGAG 
GCCAAAGATG AGAACGAGAG GTCGTCCATC AGCCCCATGG ATGAACCTGT GCCTGACTCA 
GAGTCTCCTA TCGAGAAAGT TCTGTCTCCG TTACGCAGCC CTCCCCTTAT TGGATCCGAG 
TCCGCATATG AAGACTTCCT GAGTGCGGAT GACAAGGCTC TTGGCAGACG TTCAGAAAGC 
CCCTTTGAAG GGAAGAATGG AAAGCAAGGC TTCTCAGACA AAGAAAGCCC AGTTTCTGAC 
CTGACTTCCG ATCTTTACCA AGACAAGCAG GAAGAGAAAA GCGCGGGCTT CATACCGATA 
AAGGAAGACT TTAGTCCAGA AAAGAAAGCC AGCGATGCTG AAATCATGAG TTCTCAATCA 
GCTCTGGCTT TGGATGAAAG GAAACTGGGA GGAGATGGAT CTCCAACGCA AGTAGATGTC 
AGTCAGTTTG GCTCTTTCAA AGAAGACACC AAGATGTCCA TTTCGGAAGG CACCGTTTCA 
GACAAGTCCG CCACGCCTGT GGATGAGGGC GTGGCCGAAG ACACCTATTC ACACATGGAA 
GGTGTGGCCT CAGTGTCAAC CGCCTCTGTG GCTACCAGCT CGTTTCCAGA GCCAACCACA 
GATGACGTGT CTCCTTCTCT CCACGCTGAA GTGGGCTCTC CACATTCCAC AGAGGTGGAT 
GACTCCCTGT CGGTGTCGGT GGTGCAAACA CCAACTACTT TCCAGGAAAC AGAAATGTCT 
CCGTCTAAAG AAGAGTGCCC AAGACCAATG TCGATTTCTC CTCCTGACTT CTCCCCTAAG 
ACAGCCAAAT CCAGGACACC AGTTCAAGAT CACCGATCCG AACAGTCTTC AATGTCTATT 
GAATTCGGTC AGGAATCCCC CGAGCATTCT CTTGCTATGG ACTTTAGTCG GCAGTCTCCA 
GACCACCCTA CTGTGGGTGC TGGTATGCTT CACATCACCG AAAATGGGCC AACTGAGGTG 
GACTACAGTC CCTCCGATAT CCAGGACTCT AGTTTGTCAC ATAAGATTCC GCCGACAGAA 
GAGCCATCCT ACACCCAGGA TAATGATCTG TCCGAGCTCA TCTCTGTGTC TCAGGTGGAG 
GCTTCCCCAT CCACCTCTTC TGCTCACACT CCTTCTCAGA TAGCCTCTCC TCTTCAGGAA 
GACACTCTCT CTGATGTCGT TCCTCCCAGA GATATGTCCT TATATGCCTC GCTTGCGTCT 
GAGAAAGTGC AGAGCCTGGA AGGAGAGAAA CTCTCTCCAA AATCCGATAT TTCTCCGCTC 
ACCCCTCGAG AGTCCTCACC TACATATTCA CCTGGCTTTT CAGATTCTAC CTCTGGAGCT 
AAAGAGAGTA CAGCGGCTTA CCAAACCTCC TCTTCCCCAC CAATAGATGC AGCAGCCGCA 
GAGCCCTACG GCTTCCGCTC CTCAATGTTA TTTGATACAA TGCAGCATCA CCTGGCCTTG 
AGTAGAGATT TGACCACATC TAGTGTGGAG AAGGACAATG GAGGGAAGAC ACCCGGTGAC 
TTTAACTATG CCTATCAAAA GCCCGAGAGC ACCACCGAAT CCCCAGATGA AGAAGATTAT 
GACTATGAAT CTCACGAGAA AACCATCCAG GCCCACGATG TGGGTGGTTA CTACTATGAG 
AAGACAGAGA GAACCATAAA ATCCCCATGT GACAGTGGAT ACTCCTATGA GACCATTGAG 
AAGACCACCA AGACCCCAGA AGATGGTGGC TACTCCTGTG AAATTACCGA GAAAACCACT 
CGGACCCCTG AAGAGGGCGG GTACTCGTAT GAGATCAGCG AGAAGACAAC ACGAACCCCT 
GAAGTAAGTG GCTACACCTA TGAGAAGACC GAGAGGTCCA GAAGGCTCCT CGATGACATT 
AGCAATGGCT ACGATGACAC TGAGGATGGT GGCCACACAC TTGGCGACTG TAGCTATTCC 
TACGAAACCA CTGAGAAAAT TACCAGCTTT CCTGAATCTG AAAGCTATTC CTATGAGACA 
ACTACAAAAA CAACACGGAG TCCAGACACC TCTGCATACT GTTACGAGAC CATGGAGAAG 
ATCACCAAGA CCCCACAGGC ATCCACATAC TCCTATGAGA CCTCAGACCG ATGCTACACT 
CCAGAAAGGA AGTCCCCCTC GGAGGCACGC CAGGATGTTG ACTTGTGTCT GGTGTCCTCC 
TGTGAATTCA AGCATCCCAA GACCGAGCTC TCACCTTCCT TCATTAATCC AAACCCTCTC 
GAGTGGTTTG CTGGGGAAGA GCCCACTGAA GAATCTGAGA AGCCTCTCAC TCAGTCTGGA 
GGAGCCCCCC CACCTTCAGG AGGAAAACAA CAGGGCAGAC AATGCGATGA AACTCCACCC 
ACCTCAGTCA GTGAGTCAGC TCCATCCCAG ACGGACTCTG ATGTTCCCCC AGAGACAGAA 
GAGTGCCCCT CCATCACAGC TGATGCCAAC ATTGACTCTG AAGATGAGTC AGAAACCATC 
CCCACAGACA AAACGGTTAC GTACAAACAC ATGGACCCGC CTCCAGCCCC CATGCAAGAC 
CGAAGCCCTT CTCCTCGCCA CCCTGATGTG TCCATGGTGG ATCCAGAGGC CTTGGCTATT 
GAGCAGAACC TAGGCAAGGC TCTGAAAAAG GATCTGAAGG AGAAGGCCAA GACCAAGAAA 
CCAGGCACAA AGACCAAGTC CTCTTCACCT GTCAAAAAGG GTGATGGGAA GTCCAAGCCT 
TCAGCAGCTT CCCCCAAACC AGGAGCCTTG AAGGAATCCT CTGACAAGGT GTCCAGAGTG 
GCTTCTCCCA AGAAGAAAGA GTCTGTGGAG AAAGCTATGA AGACCACCAC CACTCCTGAG 
GTCAAAGCCA CACGAGGGGA AGAGAAGGAC AAGGAAACTA AGAATGCAGC CAATGCTTCT 
GCATCCAAGT CAGTGAAGAC TGCAACAGCA GGACCAGGAA CCACTAAGAC GGCCAAGTCG 
TCCACCGTGC CTCCCGGCCT CCCTGTGTAT TTGGACCTCT GCTATATTCC CAACCACAGC 
AACAGTAAGA ATGTCGATGT TGAGTTTTTC AAGAGAGTGA GGTCATCTTA CTACGTGGTG 
AGTGGGAACG ACCCTGCCGC GGAGGAGCCC AGCCGGGCTG TCCTGGATGC CTTGTTGGAA 
GGGAAGGCTC AGTGGGGAAG CAACATGCAG GTGACTCTGA TCCCAACACA TGACTCTGAG 
GTGATGAGGG AGTGGTACCA GGAGACCCAC GAGAAGCAGC AAGACCTCAA CATCATGGTC 
CTAGCAAGCA GTAGTACAGT GGTCATGCAA GACGAGTCCT TCCCTGCATG CAAGATAGAA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MAP1B Light Chain Modulates Synaptic Transmission via AMPA Receptor Intracellular Trapping.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(41)9945-9963(2017 Oct)
Palenzuela R,Guti?rrez Y,Draffin JE,Lario A,Benoist M,Esteban JA

Dynamic Palmitoylation Targets MAP6 to the Axon to Promote Microtubule Stabilization during Neuronal Polarization.
Neuron94(4)809-825.e7(2017 May)
Tortosa E,Adolfs Y,Fukata M,Pasterkamp RJ,Kapitein LC,Hoogenraad CC

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Neuron94(2)347-362.e7(2017 Apr)
Gumy LF,Katrukha EA,Grigoriev I,Jaarsma D,Kapitein LC,Akhmanova A,Hoogenraad CC

Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(11)4587-98(2015 Mar)
Liu Y,Lee JW,Ackerman SL

Interaction of tubby-like protein-1 (Tulp1) and microtubule-associated protein (MAP) 1A and MAP1B in the mouse retina.
Advances in experimental medicine and biology801511-8(2014)
Grossman GH,Beight CD,Ebke LA,Pauer GJ,Hagstrom SA

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Map1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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