Glo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Glo1
Entrez Gene ID	294320
Full Name	glyoxalase 1
General protein information	Preferred Names glyoxalase 1 Names lactoylglutathione lyase S-D-lactoylglutathione methylglyoxal lyase aldoketomutase glx I glyoxalase I glyoxylase 1 ketone-aldehyde mutase methylglyoxalase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	predicted to have lactoylglutathione lyase activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_207594.2	NP_997477.1	lactoylglutathione lyase

Pathways from BioSystems

Homologies

	GLO1	XP_001117098.1
Canis lupus familiaris (dog)	GLO1	XP_532129.3
Homo sapiens (human)	GLO1	NP_006699.2
Bos taurus (cattle)	LOC101905254	XP_005208451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012071	XP_320453.4
Eremothecium gossypii	AGOS_ADR286C	NP_984382.1
Magnaporthe oryzae (rice blast fungus)	MGG_11543	XP_003718417.1
Arabidopsis thaliana (thale cress)	AT1G08110	NP_001030995.1
Oryza sativa (rice)	Os05g0295800	NP_001055113.1
Bos taurus (cattle)	GLO1	NP_001076965.1
Drosophila melanogaster (fruit fly)	CG1707	NP_610270.1
Saccharomyces cerevisiae (baker's yeast)	GLO1	NP_013710.1
Kluyveromyces lactis	KLLA0F06226g	XP_455362.1
Neurospora crassa	NCU04815	XP_960441.1
Mus musculus (house mouse)	Glo1	NP_079650.3
Rattus norvegicus (Norway rat)	Glo1	NP_997477.1
Danio rerio (zebrafish)	glo1	NP_998316.1
Pan troglodytes (chimpanzee)	GLO1	XP_001173775.1
Gallus gallus (chicken)	GLO1	XP_419481.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012072	XP_320454.4
Schizosaccharomyces pombe (fission yeast)	glo1	NP_596010.1
Xenopus tropicalis (tropical clawed frog)	glo1	NP_001025545.1

Gene Ontology
Bibliography

Related articles in PubMed

Intracellular Accumulation of Methylglyoxal by Glyoxalase 1 Knock Down Alters Collagen Homoeostasis in L6 Myoblasts.
Stratmann B, Goldstein B, Thornalley PJ, Rabbani N, Tschoepe D
International journal of molecular sciences18(3)(2017 Feb)

Expression of glyoxalase-I is reduced in cirrhotic livers: A possible mechanism in the development of cirrhosis.
Hollenbach M, Thonig A, Pohl S, Ripoll C, Michel M, Zipprich A
PloS one12(2)e0171260(2017)

Glyoxalase-1 overexpression partially prevents diabetes-induced impaired arteriogenesis in a rat hindlimb ligation model.
Brouwers O, Yu L, Niessen P, Slenter J, Jaspers K, Wagenaar A, Post M, Miyata T, Backes W, Stehouwer C, Huijberts M, Schalkwijk C
Glycoconjugate journal33(4)627-30(2016 Aug)

Temporal dynamics of glyoxalase 1 in secondary neuronal injury.
Pieroh P, Koch M, Wagner DC, Boltze J, Ehrlich A, Ghadban C, Hobusch C, Birkenmeier G, Dehghani F
PloS one9(2)e87364(2014)

Impact of GLO1 knock down on GLUT4 trafficking and glucose uptake in L6 myoblasts.
Engelbrecht B, Stratmann B, Hess C, Tschoepe D, Gawlowski T
PloS one8(5)e65195(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased biosynthesis of collagens 1 and 4 was detected at mRNA and protein level following knockdown of glyoxalase 1 in L6 myoblasts.
Title: Intracellular Accumulation of Methylglyoxal by Glyoxalase 1 Knock Down Alters Collagen Homoeostasis in L6 Myoblasts.

These results show the importance of Glo-I as major detoxifying enzyme for methylglyoxal in cirrhosis. The reduced expression of Glo-I in cirrhosis demonstrates a possible explanation for increased inflammatory injury and suggests a "vicious circle" in liver disease.
Title: Expression of glyoxalase-I is reduced in cirrhotic livers: A possible mechanism in the development of cirrhosis.

Diabetes-induced impairment of arteriogenic adaptation can be partially rescued by overexpressing of GLO-I, indicating a role of AGEs in diabetes-induced impaired collateral formation
Title: Glyoxalase-1 overexpression partially prevents diabetes-induced impaired arteriogenesis in a rat hindlimb ligation model.

Data suggest that the high dynamics of glyoxalase 1 expression and distribution following neuronal injury may indicate a role of Glyoxalase 1.
Title: Temporal dynamics of glyoxalase 1 in secondary neuronal injury.

GLO1 knock down augmented GLUT4 level on the cell surface of L6 myoblasts at least in part through reduction of GLUT4 internalization.
Title: Impact of GLO1 knock down on GLUT4 trafficking and glucose uptake in L6 myoblasts.

The following Glo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Glo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05577	NM_207594.2 Latest version!	Rattus norvegicus glyoxalase 1 (Glo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Glo1 ( NM_207594.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997477.1 Rattus norvegicus glyoxalase 1 (Glo1), mRNA.

Price & Availability↑

CloneID	ORa05577
Clone ID Related Accession (Same CDS sequence)	NM_207594.2
Accession Version	NM_207594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	lactoylglutathione lyase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061570.1. On Aug 31, 2012 this sequence version replaced NM_207594.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGC CACAGCCAGC GTCCAGTGGT CTTACTGATG AGGCCGCCCT CAGTTGCTGC 
TCTGACCCGG ATCCAAGCAC CAAGGATTTT CTACTGCAGC AAACGATGCT GAGAATTAAG 
GACCCTAAGA AGTCCCTGGA TTTTTATACG AGGGTTCTTG GACTGACGCT TCTCCAGAAG 
CTTGACTTCC CTTCTATGAA GTTCTCGCTC TATTTCTTAG CGTACGAGGA TAAGAACGAT 
ATCCCCAAGG ACAAGACGGA GAGGACAGCG TGGGCTTTTT CCAGAAAGGC TACCCTGGAG 
CTCACACACA ACTGGGGCAC TGAAGATGAC GAGACGCAGA GTTACCACAA CGGCAACTCG 
GACCCTCGGG GATTTGGCCA CATTGGGATT GCTGTTCCTG ATGTCTACGA GGCCTGTAAG 
AGATTTGAAG AACTAGGTGT CAAGTTTGTG AAGAAGCCTG ATGATGGGAA AATGAAAGGT 
CTGGCGTTCG TTCAAGATCC TGATGGCTAC TGGATTGAAA TTCTGAATCC TAACAAAATG 
GCAACAATTA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997477.1
CDS	78..632
Translation	MAEPQPASSGLTDEAALSCCSDPDPSTKDFLLQQTMLRIKDPKKSLDFYTRVLGLTLLQKLDFPSMKFSLYFLAYEDKNDIPKDKTERTAWAFSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYEACKRFEELGVKFVKKPDDGKMKGLAFVQDPDGYWIEILNPNKMATII

Target ORF information:

RefSeq Version	NM_207594.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glyoxalase 1 (Glo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207594.2

ORF Insert Sequence:

ATGGCAGAGC CACAGCCAGC GTCCAGTGGT CTTACTGATG AGGCCGCCCT CAGTTGCTGC 
TCTGACCCGG ATCCAAGCAC CAAGGATTTT CTACTGCAGC AAACGATGCT GAGAATTAAG 
GACCCTAAGA AGTCCCTGGA TTTTTATACG AGGGTTCTTG GACTGACGCT TCTCCAGAAG 
CTTGACTTCC CTTCTATGAA GTTCTCGCTC TATTTCTTAG CGTACGAGGA TAAGAACGAT 
ATCCCCAAGG ACAAGACGGA GAGGACAGCG TGGGCTTTTT CCAGAAAGGC TACCCTGGAG 
CTCACACACA ACTGGGGCAC TGAAGATGAC GAGACGCAGA GTTACCACAA CGGCAACTCG 
GACCCTCGGG GATTTGGCCA CATTGGGATT GCTGTTCCTG ATGTCTACGA GGCCTGTAAG 
AGATTTGAAG AACTAGGTGT CAAGTTTGTG AAGAAGCCTG ATGATGGGAA AATGAAAGGT 
CTGGCGTTCG TTCAAGATCC TGATGGCTAC TGGATTGAAA TTCTGAATCC TAACAAAATG 
GCAACAATTA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intracellular Accumulation of Methylglyoxal by Glyoxalase 1 Knock Down Alters Collagen Homoeostasis in L6 Myoblasts.
International journal of molecular sciences18(3)(2017 Feb)
Stratmann B,Goldstein B,Thornalley PJ,Rabbani N,Tschoepe D

Expression of glyoxalase-I is reduced in cirrhotic livers: A possible mechanism in the development of cirrhosis.
PloS one12(2)e0171260(2017)
Hollenbach M,Thonig A,Pohl S,Ripoll C,Michel M,Zipprich A

Glyoxalase-1 overexpression partially prevents diabetes-induced impaired arteriogenesis in a rat hindlimb ligation model.
Glycoconjugate journal33(4)627-30(2016 Aug)
Brouwers O,Yu L,Niessen P,Slenter J,Jaspers K,Wagenaar A,Post M,Miyata T,Backes W,Stehouwer C,Huijberts M,Schalkwijk C

Temporal dynamics of glyoxalase 1 in secondary neuronal injury.
PloS one9(2)e87364(2014)
Pieroh P,Koch M,Wagner DC,Boltze J,Ehrlich A,Ghadban C,Hobusch C,Birkenmeier G,Dehghani F

Impact of GLO1 knock down on GLUT4 trafficking and glucose uptake in L6 myoblasts.
PloS one8(5)e65195(2013)
Engelbrecht B,Stratmann B,Hess C,Tschoepe D,Gawlowski T

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Glo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報